Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,182,992 A→T 10.1% F248I (TTC→ATC)  PP_4563 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,182,9920AT10.1% 211.9 / 13.3 79F248I (TTC→ATC) PP_4563hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (35/36);  new base T (4/4);  total (39/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.21e-01

CGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTGAACAGCTTGCTGATCAACGCAGGGTCACTCTTGTCGCACACCCCCAACACCCCGCCTTCGGCTTCCAGGCGCGCCATGATCTGCTCTTGCAGGGTGTCCGCCAGGGCGGCGCCTACCGGCTGCAGGCTCAGTGAAATCTTGCCGTCCT  >  NC_002947/5182844‑5183138
                                                                                                                                                    |                                                                                                                                                  
cGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTGaa                                                                                                                                                    <  2:247655/149‑1 (MQ=255)
 gTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTGaa                                                                                                                                                    >  3:39567/1‑148 (MQ=255)
  tCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTGAACa                                                                                                                                                  <  8:167368/149‑1 (MQ=255)
    cGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTGAACAGc                                                                                                                                                >  4:120335/1‑149 (MQ=255)
       gTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTGAACAGCTTg                                                                                                                                             >  1:298174/1‑149 (MQ=255)
         gcgcagcagcTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTGAACGGCTTGCt                                                                                                                                           <  3:43285/145‑1 (MQ=255)
          tcgcgcagcTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTGAACAGCTTGCTg                                                                                                                                          <  6:416803/145‑1 (MQ=255)
             agcagcTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTGAACAGCTTGCTGAt                                                                                                                                        >  7:140167/1‑148 (MQ=255)
                agcTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTGATCAGCAAGCTGTTCAAc                                                                                                                                    <  8:266350/149‑1 (MQ=255)
                agcTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTGATCAGCAAGCTGTTCAAc                                                                                                                                    <  6:281606/149‑1 (MQ=255)
                  cTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTGAACAGCTTGCTGATCAACg                                                                                                                                   >  7:161399/1‑148 (MQ=255)
                     gACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTGATCAGCAAGCTGTTCAACgtca                                                                                                                                <  3:82308/148‑4 (MQ=255)
                     gACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATCACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTGAACAGCTTGCTGATCAACGCa                                                                                                                                 <  8:264172/147‑1 (MQ=255)
                       cTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTGAACAGCTTGCTGATCAACGCAGGGt                                                                                                                             >  3:276089/1‑149 (MQ=255)
                        ttCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTTAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTGAACAGCTTGCTGATCAACGCAGGGt                                                                                                                             >  2:401749/1‑148 (MQ=255)
                        ttCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTGaa                                                                                                                                                    <  3:352471/125‑1 (MQ=255)
                        ttCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTGaa                                                                                                                                                    >  4:352471/1‑125 (MQ=255)
                        ttCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTGAACAGCTTGCTGATCAACGCAGGGt                                                                                                                             >  7:273473/1‑148 (MQ=255)
                        ttCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTGAACAGCTTGCTGATCAACGCAGGGt                                                                                                                             >  5:232586/1‑148 (MQ=255)
                           cGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTGAACAGCTTGCTGATCAACGCAGGGTCAct                                                                                                                         <  6:819/149‑1 (MQ=255)
                            gCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTGATCAGCAAGCTGTTCaa                                                                                                                                     >  8:222423/1‑136 (MQ=255)
                            gCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTGATCAGCAAGCTGTTCaa                                                                                                                                     <  7:222423/136‑1 (MQ=255)
                            gCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTGAACAGCTTGCTGATCAACGCAGGGTCActc                                                                                                                        <  6:19108/149‑1 (MQ=255)
                            gCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTGAACAGCTTGCTGATCAACGCAGGGTCActc                                                                                                                        <  6:13123/149‑1 (MQ=255)
                            gCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTGAACAGCTTGCTGATCAACGCAGGGTCActc                                                                                                                        <  5:161198/149‑1 (MQ=255)
                              aaCTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTGAACAGCTTGCTGATCAACGCAGGGTCACTCtt                                                                                                                      <  4:39567/149‑1 (MQ=255)
                                cTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTGAACAGCTTGCTGATCAACGCAGGGTCACTCTTGt                                                                                                                    <  6:244245/149‑1 (MQ=255)
                                 ttCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTGAACAGCTTGCTGATCAACGCAGGGTCACTCTTGTc                                                                                                                   >  8:135163/1‑149 (MQ=255)
                                  tCGTACAGGCGTAAGGCCTTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTGAACAGCTTGCTGATCAACGCAGGGTCACTCTTGTc                                                                                                                   <  8:140167/148‑1 (MQ=255)
                                        aGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTGAACAGCTTGCTGATCAACGCAGGGTCACTCTTGTCGCACAcc                                                                                                            >  8:258272/1‑149 (MQ=255)
                                          gCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTGAACAGCTTGCTGATCAACGCAGGGTCACTCTTGTc                                                                                                                   >  5:25984/1‑140 (MQ=255)
                                          gCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTGAACAGCTTGCTGATCAACGCAGGGTCACTCTTGTc                                                                                                                   <  6:25984/140‑1 (MQ=255)
                                           cGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTGAACAGCTTGCTGATCAACGCAggg                                                                                                                              <  5:294529/128‑1 (MQ=255)
                                           cGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTGAACAGCTTGCTGATCAACGCAggg                                                                                                                              >  6:294529/1‑128 (MQ=255)
                                            gTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTGAACa                                                                                                                                                  <  5:197035/107‑1 (MQ=255)
                                            gTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTGAACa                                                                                                                                                  >  6:197035/1‑107 (MQ=255)
                                                ggCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTGAACAGCTTGCTGATCAACGCAGGGTCACTCTTGTCGCACACCCCCAacac                                                                                                    <  4:276089/149‑1 (MQ=255)
                                                ggCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTGAACAGCTTGCTGATCAACGCAGGGTCACTCTTGTCGCACACCCCCAacac                                                                                                    >  7:271340/1‑149 (MQ=255)
                                                       aGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTGAACAGCTTGCTGATCAACGCAGGGTCACTCTTGTCGCACACCCCCAACACCCCGcc                                                                                              <  7:135163/148‑1 (MQ=255)
                                                       aGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTGAACAGCTTGCTGATCAACGCAGGGTCACTCTTGTCGCACACCCCCAACACCCCGCCt                                                                                             >  6:181316/1‑149 (MQ=255)
                                                         gCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTGAACAGCTTGCTGATCAACGCAGGGTCACTCTTGTCGCCCACCCCCAACACCCCGCCTTc                                                                                           >  5:329327/1‑149 (MQ=255)
                                                              ctcGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTGAACAGCTTGCTGATCAACGCAGGGTCACTCTTGTCGCACACCCCCAACACACCGCCTTCGGCtt                                                                                      >  2:237877/1‑149 (MQ=255)
                                                               tcGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTGAACAGCTTGCTGATCAACGCAGGGTCACTCTTGTCGCACACCCCCAACACCCCGCCTTCGGCTTc                                                                                     >  5:35673/1‑149 (MQ=255)
                                                                 gATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTGAACAGCTTGCTGATCAACGCAGGGTCACTCTTGTCGCACACCCCCAACAcccc                                                                                                 >  5:210118/1‑135 (MQ=255)
                                                                 gATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTGAACAGCTTGCTGATCAACGCAGGGTCACTCTTGTCGCACACCCCCAACAcccc                                                                                                 <  6:210118/135‑1 (MQ=255)
                                                                   cctgATCGTCATCCATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTGAACAGCTTGCTGATCAACGCAGGGTCACTCTTGTCGCACACCCCCAACACCCCGCCTTCGGCTTCCAgg                                                                                 >  8:92478/4‑149 (MQ=255)
                                                                     cGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTGAACAGCTTGCTGATCAACGCAGGGTCACTCTTGTCGCACACCCCCAACACCCCGCCTTCGGCTTCCAGgc                                                                                <  3:120335/148‑1 (MQ=255)
                                                                      gATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTGAACAGCTTGCTGATCAACGCAGGGTCACTCTTGTCGCACACCCCCAACACCCCGCCTTCGGCTTCCAGgcgc                                                                              <  3:340507/149‑1 (MQ=255)
                                                                      gATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTGAACAGCTTGCTGATCAACGCAGGGTCACTCTTGTCGCACACCCCCAACACCCCGCCTTCGGCTTCCAGgcgc                                                                              >  4:340507/1‑149 (MQ=255)
                                                                        tCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTGAACAGCTTGCTGATCAACGCAGGGTCACTCTTGTCGCACACCCCCAACACCCCGCCTTCGGCTTCCAGgcgcgc                                                                            <  3:48896/149‑1 (MQ=255)
                                                                                     cGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTGAACAGCTTGCTGATCAACGCAGGGTCACTCTTGTCGCACACCCCCAACACCCCGCCTTCGGCTTCCAGGCGCGCCATGATCTGctct                                                               <  8:273473/149‑1 (MQ=255)
                                                                                      gATCAGGACCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTGAACAGCTTGCTGATCAACGCAGGGTCACTCTTGTCGCACACCCCCAACACCCCGCCTTCGGCTTCCAGGCGCGCCATGATCTGCTCtt                                                              >  8:352026/1‑149 (MQ=255)
                                                                                          aGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTGAACAGCTTGCTGATCAACGCAGGGTCACTCTTGTCGCACACCCCCAACACCCCGCCTTCGGCTTCCAGGCGCGCCATGATCTGCTCTTGCAg                                                          <  6:232586/149‑1 (MQ=255)
                                                                                           ggCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTGAACAGCTTGCTGATCAACGCAGGGTCACTCTTGTCGCACACCCCCAACACCCCGCCTTCGGCTTCCAGGCGCGCCATGATCTGCTCTTGCAgg                                                         <  6:385145/149‑1 (MQ=255)
                                                                                           ggCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTGAACAGCTTGCTGATCAACGCAGGGTCACTCTTGTCGCACACCCCCAACACCCCGCCTTCGGCTTCCAGGCGCGCCATGATCTGCTCTTGCAgg                                                         <  4:25611/149‑1 (MQ=255)
                                                                                              ccTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTGAACAGCTTGCTGATCAACGCAGGGTCACTCTTGTCGCACACCCCCAACACCCCGCCTTCGGCTTCCAGGCGCGCCATGATCTGCTCTTGCAGGgtg                                                      >  6:14615/1‑149 (MQ=255)
                                                                                                  cTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTGAACAGCTTGCTGATCAACGCAGGGTCACTCTTGTCGCACACCCCCAACACCCCGCCTTCGGCTTCCAGGCGCGCCATGATCTGCTCTTGCAGGGTGTcc                                                   <  5:14615/148‑1 (MQ=255)
                                                                                                  cTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTGAACAGCTTGCTGATCAACGCAGGGTCACTCTTGTCGCACACCCCCAACACCCCGCCTTCGGCTTCCAGGCGCGCCATGATCTGCTCTTGCAGGGTGTcc                                                   <  8:161399/148‑1 (MQ=255)
                                                                                                     gAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTGAACAGCTTGCTGATCAACGCAGGGTCACTCTTGTCGCACACCCCCAACACCCCGCCTTCGGCTTCCAGGCGCGCCATGATCTGCTCTTGCAGGGTGTCCGc                                                 <  5:181316/147‑1 (MQ=255)
                                                                                                      aacaacCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTGAACAGCTTGCTGATCAACGCAGGGTCACTCTTGTCGCACACCCCCAACACCCCGCCTTCGGCTTCCAGGCGCGCCATGATCTGCTCTTGCAGGGTGTCCGCCa                                               <  7:258272/148‑1 (MQ=255)
                                                                                                      aacaacCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTGAACAGCTTGCTGATCAACGCAGGGTCACTCTTGTCGCACACCCCCAACACCCCGCCTTCGGCTTCCAGGCGCGCCATGATCTGCTCTTGCAGGGTGTCCGCCa                                               <  2:298174/148‑1 (MQ=255)
                                                                                                      aacaacCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTGAACAGCTTGCTGATCAACGCAGGGTCACTCTTGTCGCACACCCCCAACACCCCGCCTTCGGCTTCCAGGCGCGCCATGATCTGCTCTTGCAGGGTGTCCGCCAg                                              >  6:88878/1‑149 (MQ=255)
                                                                                                           cccGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTGAACAGCTTGCTGATCAACGCAGGCTCACTCTTGTAGCACACCCCCAACCCCCCGCCTTCGGCTTCCAGGCGCGCCATGATCTGCTCATGCAgg                                                         >  7:376280/1‑133 (MQ=255)
                                                                                                                 aaTGGCCTTCTTGAAGTTGCCCTTGCTGACGTTGAACAGCTTGCTGATCAACGCAGGGTCACTCTTGTCGCACACCCCCAACACCCCGCCTTCGGCTTCCAGGCGCGCCATGATCTGCTCTTGCAGGGTGTCCGCCAGGGCGGCGCCTa                                   <  6:35673/149‑1 (MQ=255)
                                                                                                                       cttcttGAAGTTGCCCTTGCTGACGTTGAACAGCTTGCTGATCAACGCAGGGTCACTCTTGTCGCACACCCCCAACACCCCGCCTTCGGCTTCCAGGCGCGCCATGATCTGCTCTTGCAgg                                                         <  3:103244/121‑1 (MQ=255)
                                                                                                                        ttcttGAAGTTGCCCTTGCTGACGTTGAACAGCTTGCTGATCAACGCAGGGTCACTCTTGTCGCACACCCCCAACACCCCGCCTTCGGCTTCCAGGCGCGCCATGATCTGCTCTTGCAGGGTGTCCGCCAGGGCGGCGCCTACCGGCTg                            >  8:232187/1‑149 (MQ=255)
                                                                                                                             gAAGTTGCCCTTGCTGACGTTGAACAGCTTGCTGATCAACGCAGGGTCACTCTTGTCGCACACCCCCAACACCCCGCCTTCGGCTTCCAGGCGCGCCATGATCTGCTCTTGCAGGGTGTCCGCCAGGGCGGCGCCTACCGGCTGCAGGc                       >  5:387299/1‑149 (MQ=255)
                                                                                                                             gAAGTTGCCCTTGCTGACGTTGAACAGCTTGCTGATCAACGCAGGGTCACTCTTGTCGCACACCCCCAACACCCCGCCTTCGGCTTCCAGGCGCGCCATGATCTGCTCTTGCAGGGTGTCCGCCAGGGCGGCGCCTACCGGCTGCAGGc                       >  1:188698/1‑149 (MQ=255)
                                                                                                                               aGTTGCCCTTGCTGACGTTGAACAGCTTGCTGATCAACGCAGGGTCACTCTTGTCGCACACCCCCAACACCCCGCCTTCGGCTTCCAGGCGCGCCATGATCTGCTCTTGCAGGGTGTCCGCCAGGGCGGCGCCTACCGGCTGCAGGCt                      <  4:84452/148‑1 (MQ=255)
                                                                                                                                      cTTGCTGACGTTGAACAGCTTGCTGATCAACGCAGGGTCACTCTTGTCGCACACCCCCAACACCCCGCCTTCGGCTTCCAGGCGCGCCATGATCTGCTCTTGCAGGGTGTCCGCCAGGGCGGCGCCTACCGGCTGCAGGCTCAGTGaaa              <  2:125721/149‑1 (MQ=255)
                                                                                                                                         gCTGACGTTGAACAGCTTGCTGATCAACGCAGGGTCACTCTTGTCGCACACACGCCACACCCCGCCTTCGGCTTCCAGGCGCGCCATGATCTGCTCTTGCAGGGTGTCCGCCAGGGCGGCGCCTACCGGCTGCAGGCTCAGTGAAATCt           >  1:103877/1‑149 (MQ=255)
                                                                                                                                           tGACGTTGAACAGCTTGCTGATCAACGCTGGGTCACTCTTGTCGCACACCCCCAACACCCCGCCTTCGGCTTCCAGGCGCGCCATGATCTGCTCTTGCAGGGTGTCCGCCAGGGCGGCGCCTACCGGCTGCAGGCTCAGTGAAATCTTg         >  1:229656/1‑149 (MQ=255)
                                                                                                                                           tGACGTTGAACAGCTTGCTGATCAACGCAGGGTCACTCTTGTCGCACACCCCCAACACCCCGCCTTCGGCTTCCAGGCGCGCCATGATCTGCTCTTGCAGGGTGTCCGCCAGGGCGGCGCCTACCGGCTGCAGGCTCAGTGaa               <  3:382052/143‑1 (MQ=255)
                                                                                                                                           tGACGTTGAACAGCTTGCTGATCAACGCAGGGTCACTCTTGTCGCACACCCCCAACACCCCGCCTTCGGCTTCCAGGCGCGCCATGATCTGCTCTTGCAGGGTGTCCGCCAGGGCGGCGCCTACCGGCTGCAGGCTCAGTGaa               >  4:382052/1‑143 (MQ=255)
                                                                                                                                           tGACGTTGAACAGCTTGCTGATCAACGCAGGGTCACTCTTGTCGCACACCCCCAACACCCCGCCTTCGGCTTCCAGGCGCGCCATGATCTGCTCTTGCAGGGTGTCCGCCAGGGCGGCGCCTACCGGCTGCAGGCTCAGTGAAATCTTg         >  4:124767/1‑149 (MQ=255)
                                                                                                                                           ctgcGTTGATCAGCAAGCTGTTCAACGCAGGGTCACTCTTGTCGCACCCCCCCAACACCCCGCCTTCGGCTTCCAGGCGCGCCATGATCTGCTCTTGCAGGGTGTCCGCCAGGGCGGCGCCTACCGGCTGCCGGCTCAGTGAAATCTTg         >  6:81120/4‑149 (MQ=255)
                                                                                                                                           ctgcGTTGATCAGCAAGCTGTTCAACGCAGGGTCACTCTTGTCGCACACCCCCAACACCCCGCCTTCGGCTTCCAGGCGCGCCATGATCTGCTCTTGCAGGGTGTCCGCCAGGGCGGCGCCTACCGGCTGCAGGCTCAGTGAAATCTTg         >  8:334817/4‑149 (MQ=255)
                                                                                                                                            tgcGTTGATCAGCAAGCTGTTCAACGCAGGGTCACTCTTGTCGCACACCCCCAACACCCCGCCTTCGGCTTCCAGGCGCGCCATGATCTGCTCTTGCAGGGTGTCCGCCAGGGCGGCGCCTACCGGCTGCAGGCTCAGTGAAATCTTGc        >  7:133106/3‑149 (MQ=255)
                                                                                                                                             aCGTTGAACAGCTTGCTGATCAACGCAGGGTCACTCTTGTCGCACACCCCCAACACCCCGCCTTCGGCTTCCAGGCGCGCCATGATCTGCTCTTGCAGGGTGTCCGCCAGGGCGGCGCCTACCGGCTGCAGGCTCAGTGAAATCTTGcc       >  8:125674/1‑149 (MQ=255)
                                                                                                                                             aCGTTGAACAGCTTGCTGATCAACGCAGGGTCACTCTTGTCGCACACCCCCAACACCCCGCCTTCGGCTTCCAGGCGCGCCATGATCTGCTCTTGCAGGGTGTCCGCCAGGGCGGCGCCTACCGGCTGCAGGCTCAGTGAAATCTTGcc       >  4:321790/1‑149 (MQ=255)
                                                                                                                                                ttGAACAGCTTGCTGATCAACGCAGGGTCACTCTTGTCGCACACCCCCAACACCCCGCCTTCGGCTTCCAGGCGCGCCATGATCTGCTCTTGCAGGGTGTCCGCCAGGGCGGCGCCTACCGGCTGCAGGCTCAGTGAAATCTTGCCGTc    <  6:329327/149‑1 (MQ=255)
                                                                                                                                                ttGAACAGCTTGCTGATCAACGCAGGGTCACTCTTGTCGCACACCCCCAACACCCCGCCTTCGGCTTCCAGGCGCGCCATGATCTGCTCTTGCAGGGTGTCCGCCAGGGCGGCGCCTACCGGCTGCAGGCTCAGTGAAATCTTGCCGTc    <  8:271340/149‑1 (MQ=255)
                                                                                                                                                  gAACAGCTTGCTGATCAACGCAGGGTCACTCTTGTCGCACACCCCCAACACCCCGCCTTCGGCTTCCAGGCGCGCCATGATCTGCTCTTGCAGGGTGTCCGCCAGGGCGGCGCCTACCGGCTGCAGGCTCAGTGAAATCTTGCCGTCCt  >  4:389811/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
CGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTGAACAGCTTGCTGATCAACGCAGGGTCACTCTTGTCGCACACCCCCAACACCCCGCCTTCGGCTTCCAGGCGCGCCATGATCTGCTCTTGCAGGGTGTCCGCCAGGGCGGCGCCTACCGGCTGCAGGCTCAGTGAAATCTTGCCGTCCT  >  NC_002947/5182844‑5183138

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑