Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,184,117	C→T	5.5%	A85V (GCC→GTC)	PP_4564 →	lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,184,117	0	C	T	5.5%	274.7 / 2.8	89	A85V (GCC→GTC)	PP_4564	lipoprotein
Reads supporting (aligned to +/- strand):  ref base C (42/42);  new base T (2/3);  total (44/45)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTGCCGTGGTCGCTGAGCGACTACCTGGACATGAACCGCGAGCAAAGGGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAG‑GTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCGGCATGAGCGACAACCAGGTAGCGCA  >  NC_002947/5183969‑5184252                                                                                                                                                     |                                                                                                                                         gTGCCGTGGTCGCTGAGCGACTACCTGGACATGAACCGCGAGCAAAGGGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGc                                                                                                                                          >  8:176475/1‑149 (MQ=255)    ccGTGGTCGCTGAGCGACTACCTGGACATGAACCGCGAGCAAAGGGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCcga                                                                                                                                       <  8:217261/149‑1 (MQ=255)    ccGTGGTCGCTGAGCGACTACCTGGACATGAACCGCGAGCAAAGGGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCcga                                                                                                                                       <  2:171264/149‑1 (MQ=255)     cGTGGTCGCTGAGCGACTACCTGGACATGAACCGCGAGCAAAGGGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCcgac                                                                                                                                      <  3:294790/149‑1 (MQ=255)       tGGTCGCTGAGCGACTACCTGGACATGAACCGCGAGCAAAGGGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCcgacga                                                                                                                                    <  6:167504/149‑1 (MQ=255)         gTCGCTGAGCGACTACCTGGACATGAACCGCGAGCAAAGGGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCgacgac                                                                                                                                   <  1:169759/148‑1 (MQ=255)          tCGCTGAGCGACTACCTGGACATGAACCGCGAGCAAAGGGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCa                                                                                                                                 <  7:77770/149‑1 (MQ=255)            gCTGAGCGACTACCTGGACATGAACCGCGAGCAAAGGGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACCTGGTCGTCGGCGACCatg                                                                                                                               >  4:206918/1‑147 (MQ=255)                aGCGACTACCTGGACATGAACCGCGAGCAAAGGGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACCTGGTCGTCGGCGACCAT‑GTc                                                                                                                            <  3:144075/147‑1 (MQ=255)                aGCGACTACCTGGACATGAACCGCGAGCAAAGGGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAG‑GTCAc                                                                                                                          <  5:138899/149‑1 (MQ=255)                   gACTACCTGGACATGAACCGCGAGCAAAGGGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACCTGGTCGTCGGCGACCAT‑GTCAcgta                                                                                                                       <  3:206918/149‑4 (MQ=255)                    aCTACCTGGACATGAACCGCGAGCAAAGGGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAG‑GTCACCGAc                                                                                                                      <  5:352510/149‑1 (MQ=255)                         cTGGACATGAACCGCGAGCAAAGGGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAG‑GTCACCGACCAGGc                                                                                                                 >  1:62015/1‑149 (MQ=255)                         cTGGACATGAACCGCGAGCAAAGGGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAG‑GTCACCGACCAGGc                                                                                                                 >  8:207037/1‑149 (MQ=255)                         cTGGACATGAACCGCGAGCAAAGGGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAG‑GTCACCGACCAGGc                                                                                                                 >  6:213805/1‑149 (MQ=255)                          tGGACATGAACCGCGAGCAAAGGGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAG‑GTCACCGACCAGGcc                                                                                                                >  7:140320/1‑149 (MQ=255)                             aCATGAACCGCGAGCAAAGGGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAG‑GTCACCGACCAGGCCCTg                                                                                                             <  4:79421/149‑1 (MQ=255)                              aattaaCCGCGATCAAATGGCGCTTCTGGTTTTTCGGTTTCGCTTGCACCTTGCCTGGCATTGCAATACTCAACTGCCCGGTTACCTCTATTTGCTCGACCGGTTACGTTACATGGTCGCCGACTTCCAG‑GTCACCGACCAGGCCCTgc                                                                                                            <  7:255418/145‑1 (MQ=255)                               aTGAACCGCGAGCAAAGGGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAG‑GTCACCGACCAGGCCCTgca                                                                                                           <  7:207037/149‑1 (MQ=255)                                 gAACCGCGAGCAAAGGGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAG‑GTCACCGACCAGGCCCTgcag                                                                                                          >  4:86714/1‑148 (MQ=255)                                 gAACCGCGAGCAAAGGGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAG‑GTCACCGACCAGGCCCTgcag                                                                                                          >  3:352814/1‑148 (MQ=255)                                  aaCCGCGAGCAAAGGGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAG‑GTCACCGACCAGGCCCTgcagc                                                                                                         >  6:379839/1‑148 (MQ=255)                                   aCCGCGAGCAAAGGGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAG‑GTCACCGACCAGGCCCTgcagca                                                                                                        >  5:374583/1‑148 (MQ=255)                                   aCCGCGAGCAAAGGGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAG‑GTCACCGACCAGGCCCTGCAGCaa                                                                                                       <  6:308627/149‑1 (MQ=255)                                   aCCGCGAGCAAAGGGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCACCGACGACCAG‑GTCACCGACCAGGCCCTgcagca                                                                                                        >  2:202942/1‑148 (MQ=255)                                    ccGCGAGCAAAGGGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAG‑GTCACCGACCAGGCCCTgcagca                                                                                                        >  3:137094/1‑147 (MQ=255)                                    ccGCGAGCAAAGGGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAG‑GTCACCGACCAGGCCCTccagca                                                                                                        >  3:359495/1‑147 (MQ=255)                                    ccGCGAGCAAAGGGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAG‑GTCACCGACCAGGCCCTGCAGCaaa                                                                                                      <  6:31712/149‑1 (MQ=255)                                    ccGCGAGCAAAGGGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCGCGACCGGCTACGGGACATGGTCGCCGACGACCAG‑GTCACCGACCAGGCCCTGCAGCaaa                                                                                                      <  8:140320/149‑1 (MQ=255)                                     cgcgAGCAAAGGGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAG‑GTCAcc                                                                                                                         <  6:113300/129‑1 (MQ=255)                                     cgcgAGCAAAGGGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAG‑GTCAcc                                                                                                                         >  5:113300/1‑129 (MQ=255)                                     cgcgAGCAAAGGGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAG‑GTCACCGACCAGGCCCTGCAGCAAAg                                                                                                     >  4:249500/1‑149 (MQ=255)                                     cgcgAGCAAAGGGCGCTGCTGGATGATCGGTAGCGCGAGCACCTCGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCACGACCCGCTACGTGACAACGTCCCCGACGACCAG‑GTCACCGACCAGGCCCTGCAGCAAAg                                                                                                     >  3:331042/1‑149 (MQ=255)                                            aaaGGGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAG‑GTCACCGACCAGGCCCTGCAGCAAAGCACCcgc                                                                                              >  4:295671/1‑149 (MQ=255)                                             aaGGGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAG‑GTCACCGACCAGGCCCTGCAGCAAAGCACCcgc                                                                                              >  4:353561/1‑148 (MQ=255)                                               gggCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAG‑GTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAg                                                                                           >  2:318834/1‑149 (MQ=255)                                               gggCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAG‑GTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAg                                                                                           <  7:84710/149‑1 (MQ=255)                                                       ctgGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAG‑GTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGa                                                                                   <  6:177329/149‑1 (MQ=255)                                                          gatgatCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAG‑GTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAgg                                                                                 >  4:197969/1‑148 (MQ=255)                                                              atCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAG‑GTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAAtt                                                                            <  8:247753/149‑1 (MQ=255)                                                              atCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAG‑GTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAAt                                                                             <  5:379839/148‑1 (MQ=255)                                                                  gTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTGAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAG‑GTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTc                                                                        <  4:331042/149‑1 (MQ=255)                                                                       gcgAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAG‑GTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTg                                                                   >  2:125096/1‑149 (MQ=255)                                                                         gAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAG‑GTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGc                                                                 >  7:361216/1‑149 (MQ=255)                                                                            cACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAG‑GTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGa                                                              <  4:352814/149‑1 (MQ=255)                                                                               ctggcctggcATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAG‑GTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAgg                                                            >  3:188069/1‑148 (MQ=255)                                                                                   cctggcATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAG‑GTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATc                                                       <  4:359495/149‑1 (MQ=255)                                                                                   cctggcATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAG‑GTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATc                                                       <  6:374583/149‑1 (MQ=255)                                                                                    ctggcATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAG‑GTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCa                                                      >  1:57404/1‑149 (MQ=255)                                                                                      ggcATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAG‑GTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCAcc                                                    >  5:11774/1‑149 (MQ=255)                                                                                       gcATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAG‑GTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCAcc                                                    <  2:62015/148‑1 (MQ=255)                                                                                           tGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAG‑GTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTc                                               >  2:130548/1‑149 (MQ=255)                                                                                             cAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAG‑GTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCgg                                             <  5:213805/149‑1 (MQ=255)                                                                                             cAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAG‑GTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCg                                              <  3:249500/148‑1 (MQ=255)                                                                                             cAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAG‑GTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCg                                              <  4:137094/148‑1 (MQ=255)                                                                                                 aCTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAG‑GTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCAc                                         >  5:333878/1‑149 (MQ=255)                                                                                                  cTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAG‑GTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCAcc                                        <  3:197969/149‑1 (MQ=255)                                                                                                  cTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAG‑GTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCAcc                                        >  5:180688/1‑149 (MQ=255)                                                                                                   tCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAG‑GTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAg                                                                                  >  1:56529/1‑106 (MQ=255)                                                                                                   tCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAG‑GTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAg                                                                                  <  2:56529/106‑1 (MQ=255)                                                                                                   tCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAG‑GTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCAcc                                        >  2:205950/1‑148 (MQ=255)                                                                                                     aaCTGCCCGGCTACCTCGTCTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAG‑GTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCg                                       <  8:361216/147‑1 (MQ=255)                                                                                                     aaCTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAG‑GTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGaa                                     >  3:151900/1‑149 (MQ=255)                                                                                                          cccGGCTACCTCGACTGACTCGACCGGCTACGTGACATGGTCGCCGACGACCAG‑GTCACCGACCAGGCCCTGCCGCAACGCACCCGCGAGGTCCGGGAGGCAAGTGGTCGATTGGCCGAGGCAATCCCCCCTTCGGCCCCCGAActgct                                >  4:80478/1‑149 (MQ=255)                                                                                                             ggCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAG‑GTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAAt                                                        >  4:378233/1‑122 (MQ=255)                                                                                                             ggCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAG‑GTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAAt                                                        <  3:378233/122‑1 (MQ=255)                                                                                                             ggCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAG‑GTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTgcg                             >  1:20350/1‑149 (MQ=255)                                                                                                                  ccTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAG‑GTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCgg                          <  6:180688/147‑1 (MQ=255)                                                                                                                  ccTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAG‑GTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCGGc                         <  3:86714/148‑1 (MQ=255)                                                                                                                  ccTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAG‑GTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCGGCa                        <  4:73691/149‑1 (MQ=255)                                                                                                                   cTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGCGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCGGCa                        >  1:288351/1‑149 (MQ=255)                                                                                                                   cTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAG‑GTCAc                                                                                                                          <  6:321201/50‑1 (MQ=255)                                                                                                                   cTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAG‑GTCAc                                                                                                                          >  5:321201/1‑50 (MQ=255)                                                                                                                   cTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAG‑GTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCGGCAt                       >  5:67771/1‑149 (MQ=255)                                                                                                                     cGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAG‑GTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCGGCAt                       >  1:184620/1‑147 (MQ=255)                                                                                                                      gACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAG‑GTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCGGCATGAg                    <  2:57404/149‑1 (MQ=255)                                                                                                                      gACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAG‑GTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCGGCATGAg                    <  2:112800/149‑1 (MQ=255)                                                                                                                       aCTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAG‑GTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCGGCATGAGc                   <  6:11774/149‑1 (MQ=255)                                                                                                                         tGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAG‑GTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCGGCATGAGCGa                 >  8:143748/1‑149 (MQ=255)                                                                                                                           gCTCGACCGGCTACGTGACATGGTCGCCGAGGACCAG‑GTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCGGCATGAGCGAc                >  1:37819/1‑148 (MQ=255)                                                                                                                             tCGACCGGCTACGTGACATGGTCGCCGACGACCAG‑GTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCGGc                         <  4:157320/137‑1 (MQ=255)                                                                                                                             tCGACCGGCTACGTGACATGGTCGCCGACGACCAG‑GTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCGGc                         >  3:157320/1‑137 (MQ=255)                                                                                                                             tCGACCGGCTACGTGACATGGTCGCCGACGACCAG‑GTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCGGCATGAGCGACAAc             >  4:274849/1‑149 (MQ=255)                                                                                                                              cGACCGGCTACGTGACATGGTCGCCGACGACCAG‑GTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCGGCATGAGCGACAAc             >  6:170139/1‑148 (MQ=255)                                                                                                                                     cTACGTTACATGGTCGCCGACGACCAG‑GTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTCGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCGGCATGAGCGACAACCAGGTAgc     <  1:125096/149‑1 (MQ=255)                                                                                                                                        cGTGACATGGTCGCCGACGACCAG‑GTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCGGCATGAGCGACAACCAGGTAgcg    >  6:6610/1‑147 (MQ=255)                                                                                                                                        cGTGACATGGTCGCCGACGACCAG‑GTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCGGCATGAGCGACAACCAGGTAgcg    <  4:151900/147‑1 (MQ=255)                                                                                                                                        cGTGACATGGTCGCCGACGACCAG‑GTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCGGCATGAGCGACAACCAGGTAGCGCa  <  3:353561/149‑1 (MQ=255)                                                                                                                                         gTGACCTGGTCGTCGGCGACCAT‑GTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGcgcg                           >  1:87863/1‑123 (MQ=255)                                                                                                                                         gTGACCTGGTCGTCGGCGACCAT‑GTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGcgcg                           <  2:87863/123‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                         GTGCCGTGGTCGCTGAGCGACTACCTGGACATGAACCGCGAGCAAAGGGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAG‑GTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCGGCATGAGCGACAACCAGGTAGCGCA  >  NC_002947/5183969‑5184252

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑