Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,208,043 A→C 5.1% N222K (AAT→AAG)  PP_4589 ← D‑isomer specific 2‑hydroxyacid dehydrogenase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,208,0430AC 5.1% 252.4 / 3.6 79N222K (AAT→AAG) PP_4589D‑isomer specific 2‑hydroxyacid dehydrogenase family protein
Reads supporting (aligned to +/- strand):  ref base A (37/38);  new base C (2/2);  total (39/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01

GTCAGCAGCAAGCCCCAGGCGGTCCAGAACGGGTGTTGCTTGGGCAACGGCTCCTGGCGGCACACATCGATCACAGCACCGGCCAGATGACCCTCTTTCAACGCCTCGACCAGGTCGGCATCGACCACCGCCACACCACGCCCGGCATTGATGAACAAGGCCGAGGGCTGGAAGCATTTGAACAGCGCGGCGTCATACAGGTCATGAGTCGCCGGGGTATCCGGCAGCAGGTTCAGCACGTAGTCGACCTGGCCAACCAAGCGCGGCAGGTCGGCCAGCGCCGCCACCTCGACG  >  NC_002947/5207897‑5208190
                                                                                                                                                  |                                                                                                                                                   
gTCAGCAGCAAGCCCCAGGCGGTCCAGAACGGGTGTTGCTTGGGCAACGGCTCCTGGCGGCACACATCGATCACAGCACCGGCCAGATGACCCTCTTTCAACGCCTCTACCAGGTCGGCATAGAACACCGCCACAACACGACCGGCAtt                                                                                                                                                   >  2:278656/1‑149 (MQ=255)
  cagcagCAAGCCCCAGGCGGTCCAGAACGGGTGTTGCTTGGGCAACGGCTCCTGGCGGCACACATCGATCACAGCACCGGCCAGATGACCCTCTTTCAACGCCTCGACCAGGTCGGCATCGACCACCGCCACACCACGCCCGGCCTtgt                                                                                                                                                 >  2:76376/1‑148 (MQ=255)
   agcagcAAGCCCCAGGCGGTCCAGAACGGGTGTTGCTTGGGCAACGGCTCCTGGCGGCACACATCGATCACAGCACCGGCCAGATGACCCTCTTTCAACGCCTCGACCAGGTCGGCATCGACCACCGCCACACCACGCCCGGCATtgat                                                                                                                                                <  6:332939/149‑1 (MQ=255)
   agcagcAAGCCCCAGGCGGTCCAGAACGGGTGTTGCTTGGGCAACGGCTCCTGGCGGCACACATCGATCACAGCACCGGCCAGATGACCCTCTTTCAACGCCTCGACCAGGTCGGCATCGACCACCGCCACACCACGCCCGGCATtgat                                                                                                                                                >  7:234790/1‑149 (MQ=255)
     cagcaAGCCCCAGGCGGTCCAGAACGGGTGTTGCTTGGGCAACGGCTCCTGGCGGCACACATCGATCACAGCACCGGCCAGATGACCCTCTTTCAACGCCTCGACCAGGTCGGCATCGACCACCGCCACACCACGCCCGGCATtgatg                                                                                                                                               >  3:45599/1‑148 (MQ=255)
       gcaAGCCCCAGGCGGTCCAGAACGGGTGTTGCTTGGGCAACGGCTCCTGGCGGCACACATCGATCACAGCACCGGCCAGATGACCCTCTTTCAACGCCTCGACCAGGTCGGCATCGACCACCGCCACACCACGCCCGGCATTGATGaa                                                                                                                                             >  7:75981/1‑148 (MQ=255)
       gcaAGCCCCAGGCGGTCCAGAACGGGTGTTGCTTGGGCAACGGCTCCTGGCGGCACACATCGATCACAGCACCGGCCAGATGACCCTCTTTCAACGCCTCGACCAGGTCGGCATAGACCACCGCCCCACCACGCCCGGCATTGATGaa                                                                                                                                             >  1:173015/1‑148 (MQ=255)
        caAGCCCCAGGCGGTCCAGAACGGGTGTTGCTTGGGCAACGGCTCCTGGCGGCACACATCGATCACAGCACCGGCCAGATGACCCTCTTTCAACGCCTCGACCAGGTCGGCATCGACCACCGCCACACCACGCCCGGCATTGATGAAc                                                                                                                                            >  7:267664/1‑148 (MQ=255)
        caAGCCCCAGGCGGTCCAGAACGGGTGTTGCTTGGGCAACGGCTCCTGGCGGCACACATCGATCACAGCACCGGCCAGATGACCCTCTTTCAACGCCTCGACCAGGTCGGCATCGACCACCGCCACACCACGCCCGGCATTGATGAACa                                                                                                                                           <  7:111862/149‑1 (MQ=255)
            ccccAGGCGGTCCAGAACGGGTGTTGCTTGGGCAACGGCTCCTGGCGGCACACATCGATCACAGCACCGGCCAGATGACCCTCTTTCAACGCCTCGACCAGGTCGGCATCGACCACCGCCACACCACGCCCGGCATTGATGAACAAGGc                                                                                                                                       >  2:286449/1‑149 (MQ=255)
              ccAGGCGGTCCAGAACGGGTGTTGCTTGGGCAACGGCTCCTGGCGGCACACATCGATCACAGCACCGGCCAGATGACCCTCTTTCAACGCCTCGACCAGGTCGGCATCGACCACCGCCACACCACGCCCGGCATTGATGAACAAGGcc                                                                                                                                      >  3:12557/1‑148 (MQ=255)
                aGGCGGTCCAGAACGGGTGTTGCTTGGGCAACGGCTCCTGGCGGCACACATCGATCACAGCACCGGCCAGATGACCCTCTTTCAACGCCTCGACCAGGTCGGCATCGACCACCGCCACACCACGCCCGGCCTTGTTCATCAATGCCggg                                                                                                                                   <  1:76376/149‑3 (MQ=255)
                   cGGGCCAGAACGGGTGTTGCTTGGGCAACGGCTCCTGGCGGCACACATCGATCACAGCACCGGCCAGATGACCCTCTTTCAACGCCTCGACCAGGTCGGCATCGACCACCGCCACACCACGCCCGGCATTGATGAACAAGGCCGAGGGc                                                                                                                                <  6:292194/149‑1 (MQ=255)
                     gTCCAGAACGGGTGTTGCTTGGGCAACGTCTCCTGGCGGCACACATCGATCACAGCACCGTCCAGATGACCCTCTTTCAACGCCTCGACCAGGTCGGCATCGACCACCGCCACACCACGCCCGGCATTGATGAACAAGGCCGAGGGCTg                                                                                                                              <  1:278656/149‑1 (MQ=255)
                     gTCCAGAACGGGTGTTGCTTGGGCAACGGCTCCTGGCGGCACACATCGATCACAGCACCGGCCAGATGACCCTCTTTCAACGCCTCGACCAGGTCGGCATCGACCACCGCCACACCACGCCCGGCCTTGTTCATCAATGCCgg                                                                                                                                    >  6:338322/1‑142 (MQ=255)
                     gTCCAGAACGGGTGTTGCTTGGGCAACGGCTCCTGGCGGCACACATCGATCACAGCACCGGCCAGATGACCCTCTTTCAACGCCTCGACCAGGTCGGCATCGACCACCGCCACACCACGCCCGGCCTTGTTCATCAATGCCgg                                                                                                                                    <  5:338322/143‑2 (MQ=255)
                        cAGAACGGGTGTTGCTTGGGCAACGGCTCCTGGCGGCACACATCGATCACAGCACCGGCCAGATGACCCTCTTTCAACGCCTCGACCAGGTCGGCATCGACCACCGCCACACCACGCCCGGCATTGATGAACAAGGCCGAGGGCTGGaa                                                                                                                           <  6:232056/149‑1 (MQ=255)
                          gAACGGGTGTTGCTTGGGCAACGGCTCCTGGCGGCACACATCGATCACAGCACCGGCCAGATGACCCTCTTTCAACGCCTCGACCAGGTCGGCATCGACCACCGCCACACCACGCCCGGCATTGATGAACAAGGCCGAGGGCTGGAAGc                                                                                                                         <  1:292499/149‑1 (MQ=255)
                              gggTGTTGCTTGGGCAACGGCTCCTGGCGGCACACATCGATCACAGCACCGGCCAGATGACCCTCTTTCAACGCCTCGACCAGGTCGGCATCGACCACCGCCACACCACGCCCGGCATTGATGAACAAGGCCGAGGGCTGGAAGCAttt                                                                                                                     <  5:143578/149‑1 (MQ=255)
                               ggTGTTGCTTGGGCAACGGCTCCTGGCGGCACACATCGATCACAGCACCGGCCAGATGACCCTCTTTCAACGCCTCGACCAGGTCGGCATCGACCACCGCCACACCACGCCCGGCATTGATGAACAAGGCCGAGGGCTGGAAGCATTTg                                                                                                                    <  7:274777/149‑1 (MQ=255)
                                     gCTTGGGCAACGGCTCCTGGCGGCACACATCGATCACAGCACCGGCCAGATGACCCTCTTTCAACGCCTCGACCAGGTCGGCATCGACCACCGCCACACCACGCCCGGCATTGATGAACAAGGCCGAGGGCTGGAAGCATTTGAACAgc                                                                                                              >  1:399736/1‑149 (MQ=255)
                                      cTTGGGCAACGGCTCCTGGCGGCACACATCGATCACAGCACCGGCCAGATGACCCTCTTTCAACGCCTCGACCAGGTCGGCATCGACCACCGCCACACCACGCCCGGCATTGATGAACAAGGCCGAGGGCTGGAAGCATTTGAACAgcg                                                                                                             >  5:112458/1‑149 (MQ=255)
                                         gggCAACGGCTCCTGGCGGCACACATCGATCACAGCACCGGCCAGATGACCCTCTTTCAACGCCTCGACCAGGTCGGCATCGACCACCGCCACACCACGCCCGGCATTGATGAACAAGGCCGAGGGCTGGAAGCATTTg                                                                                                                    <  5:50168/139‑1 (MQ=255)
                                         gggCAACGGCTCCTGGCGGCACACATCGATCACAGCACCGGCCAGATGACCCTCTTTCAACGCCTCGACCAGGTCGGCATCGACCACCGCCACACCACGCCCGGCATTGATGAACAAGGCCGAGGGCTGGAAGCATTTg                                                                                                                    >  6:50168/1‑139 (MQ=255)
                                          ggCAACGGCTCCTGGCGGCACACATCGATCACAGCACCGGCCAGATGACCCTCTTTCAACGCCTCGACCAGGTCGGCATCGACCACCGCCACACCACGCCCGGCATTGATGAACAAGGCCGAGGGCTGGAAGCATTTGTACAGCgcggc                                                                                                         <  8:75981/149‑1 (MQ=255)
                                           gCAACGGCTCCTGGCGGCACACATCGATCACAGCACCGGCCAGATGACCCTCTTTCAACGCCTCGACCAGGTCGGCATCGACCACCGCCACACCACGCCCGGCATTGATGAACAAGGCCGAGGGCTGGAAGCATTTGAACAGCgcggcg                                                                                                        <  2:49055/149‑1 (MQ=255)
                                             aaCGGCTCCTGGCGGCACACATCGATCACAGCACCGGCCAGATGACCCTCTTTCAACGCCTCGACCAGGTCGGCATCGACCACCGCCACACCACGCCCGGCATTGATGAACAAGGCCGAGGGCTGGAAGCATTTGAACAGCGCGGCGTc                                                                                                      <  2:399736/149‑1 (MQ=255)
                                                gtcTCCTGGCGGCACACATCGATCACAGCACCGGCCAGATGACCCTCTTTCAACTCCTATACGAGTTCGGCATCTACCAGCTCCACACCACGCCCGGCATTGATGAACAAGGCCGAGGGCTGGAAGCATTTGAACAGCGCGGCGTCATa                                                                                                   <  8:320887/147‑1 (MQ=255)
                                                          ggcACACATCGATCACAGCACCGGCCAGATGACCCTCTTTCAACGCCTCGACCAGGTCGGCATCGACCACCGCCACACCACGCCCGGCATTGATGAACAAGGCCGAGGGCTGGAAGCATTTGAACAGCGCGGCGTCATACAGGTCATGa                                                                                         <  8:234790/149‑1 (MQ=255)
                                                          ggcACACATCGATCACAGCACCGGCCAGATGACCCTCTTTCAACGCCTCGACCAGGTCGGCATCGACCACCGCCACACCACGCCCGGCATTGATGAACAAGGCCGAGGGCTGGAAGCATTTGAACAGCGCGGCGTCATACAGGTCATGa                                                                                         <  4:45599/149‑1 (MQ=255)
                                                             acacaTCGATCACAGCACCGGCCAGATGACCCTCTTTCAACGCCTCGACCAGGTCGGCATCGACCACCGCCACACCACGCCCGGCATTGATGAACAAGGCCGAGGGCTGGAAGCATTTGAACAGCGCGGCGTCATACAGGTCATGAGTc                                                                                      >  7:20901/1‑149 (MQ=255)
                                                                caTCGATCACAGCACCGGCCAGATGACCCTCTTTCAACGCCTCGACCAGGTCGGCATCGACCACCGCCACACCACGCCCGGCATTGATGAACAAGGCCGAGGGCTGGAAGCATTTGAACAGCGCGGCGTCATACAGGTCAt                                                                                           <  4:143748/141‑1 (MQ=255)
                                                                caTCGATCACAGCACCGGCCAGATGACCCTCTTTCAACGCCTCGACCAGGTCGGCATCGACCACCGCCACACCACGCCCGGCATTGATGAACAAGGCCGAGGGCTGGAAGCATTTGAACAGCGCGGCGTCATACAGGTCAt                                                                                           >  3:143748/1‑141 (MQ=255)
                                                                 aTCGATCACAGCACCGGCCAGATGACCCTCTTTCAACGCCTCGACCAGGTCGGCATCGACCACCGCCACACCACGCCCGGCATTGATGAACAAGGCCGAGGGCTGGAAGCATTTGAACAGCGCGGCGTCATACAGGTCATGAGTCGCCg                                                                                  >  4:154475/1‑149 (MQ=255)
                                                                  tCGATCACAGCACCGGCCAGATGACCCTCTTTCAACGCCTGGACCAGGTCGGCATCGACGACCGCCAGACCACGCCCGGCATTGATGAACAAGGCCGAGGGCTGGAATCATTTGAACAGCGCGTCGTCATACAGTTCATGAGTCGCCgg                                                                                 <  4:71033/149‑1 (MQ=255)
                                                                    gATCACAGCACCGGCCAGATGACCCTCTTTCAACGCCTCGACCAGGTCGGCATCGACCACCGCCACACCACGCCCGGCATTGATGAACAAGGCCGAGGGCTGGAAGCATTTGAACAGCGCGGCGTCATACAGGTCATGAGTCGCCgggg                                                                               >  6:378839/1‑149 (MQ=255)
                                                                     aTCACAGCACCGGCCAGATGACCCTCTTTCAACGCCTCGACCAGGTCGGCATCGACCACCGCCACACCACGCCCGGCATTGATGAACAAGGCCGAGGGCTGGAAGCATTTGAACAGCGCGGCGTCATACAGGTCATGAGTCGCCgggg                                                                               >  2:62262/1‑148 (MQ=255)
                                                                      tCACAGCACCGGCCAGATGACCCTCTTTCAACGCCTCGACCAGGTCGGCATCGACCACCGCCACACCACGCCCGGCATTGATGAACAAGGCCGAGGGCTGGAAGCATTTGAACAGCGCGGCGTCATACAGGTCATGAGTCGCCGGGGTa                                                                             <  3:154475/149‑1 (MQ=255)
                                                                            cACCGGCCAGATGACCCTCTTTCAACGCCTCGACCAGGTCGGCATCGACCACCGCCACACCACGCCCGGCATTGATGAACAAGGCCGAGGGCTGGAAGCATTTGAACAGCGCGGCGTCATACAGGTCATGAGTCGCCGGGGTATCCgg                                                                        >  1:302156/1‑148 (MQ=255)
                                                                               cGGCCAGATGACCCTCTTTCAACGCCTCGACCAGGTCGGCATCGACCACCGCCACACCACGCCCGGCATTGATGAACAAGGCCGAGGGCTGGAAGCATTTGAACAGCGCGGCGTCATACAGGTCATGAGTCGCCGGGGTATCCGgcag                                                                     >  8:237349/1‑148 (MQ=255)
                                                                                 gCCAGATGACCCTCTTTCAACGCCTCGACCAGGTCGGCATCGACCACCGCCACACCACGCCCGGCATTGATGAACAAGGCCGAGTGCTGGAAGCATTTGAACAGCGCGGCGTCATACAGGTCATGAGTCGCCGGGGTATCCGGcagcag                                                                  <  3:392444/149‑1 (MQ=255)
                                                                                 gCCAGATGACCCTCTTTCAACGCCTCGACCAGGTCGGCATCGACCACCGCCACACCACGCCCGGCATTGATGAACAAGGCCGAGGGCTGGAAGCATTTGAACAGCGCGGCGTCATACAGGTCATGAGTCGCCGGGGTATCCGGcagcag                                                                  <  3:365493/149‑1 (MQ=255)
                                                                                  ccAGATGACCCTCTTTCAACGCCTCGACCAGGTCGGCATCGACCACCGCCACACCACGCCCGGCATTGATGAACAAGGCCGAGGGCTGGAAGCATTTGAACAGCGCGGCGTCATACAGGTCATGAGTCGCCGGGGTATCCGGCAGCAgg                                                                 <  5:12954/149‑1 (MQ=255)
                                                                                    aGATGACCCTCTTTCAACGCCTCGACCAGGTCGGCATCGACCACCGCCACACCACGCCCGGCATTGATGAACAAGGCCGAGGGCTGGAAGCATTTGAACAGCGCGGCGTCATACAGGTCATGAGTCGCCGGGGTATCCGGCAGCAGGtt                                                               >  4:218252/1‑149 (MQ=255)
                                                                                      aTGACCCTCTTTCAACGCCTCGACCAGGTCGGCATCGACCACCGCCACACCACGCCCGGCATTGATGAACAAGGCCGAGGGCTGGAAGCATTTGAACAGCGCGGCGTCATACAGGTCATGAGTCGCCGGGGTATCCGGCAGCAGGtt                                                               >  6:29503/1‑147 (MQ=255)
                                                                                      aTGACCCTCTTTCAACGCCTCGACCAGGTCGGCATCGACCACCGCCACACCACGCCCGGCATTGATGAACAAGGCCGAGGGCTGGAAGCATTTGAACAGCGCGGCGTCATACAGGTCATGAGTCGCCGGGGTATCCGGCAGCAGGTTCa                                                             <  8:78175/149‑1 (MQ=255)
                                                                                        gACCCTCTTTCAACGCCTCGACCAGGTCGGCATCGACCACCGCCACACCACGCCCGGCATTGATGAACAAGGCCGAGGGCTGGAAGCATTTGAACAGCGCGGCGTCATACAGGTCATGAGTCGCCGGGGTATCCGGCAGCAGGTTCAg                                                            >  8:206630/1‑148 (MQ=255)
                                                                                        gACCCTCTTTCAACGCCTCGACCAGGTCGGCATCGACCACCGCCACACCACGCCCGGCATTGATGAACAAGGCCGAGGGCTGGAAGCATTTGAACAGCGCGGCGTCATACAGGTCATGAGTCGCCGGGGTATCCGGCAGCAGGTTCAGc                                                           >  4:351039/1‑149 (MQ=255)
                                                                                           ccTCTTTCAACGCCTCGACCAGGTCGGCATCGACCACCGCCACACCACGCCCGGCATTGATGAACAAGGCCGAGGGCTGGAAGCATTTGAACAGCGCGGCGTCATACAGGTCATGAGTCGCCGGGGTATCCGGCAGCAGGTTCAGCACg                                                        >  4:197246/1‑149 (MQ=255)
                                                                                            ctctTTCAACGCCTCGACCAGGTCGGCATCGACCACCGCCACACCACGCCCGGCATTGATGAACAAGGCCGAGGGCTGGAAGCATTTGAACAGCGCGGCGTc                                                                                                      <  8:63382/102‑1 (MQ=255)
                                                                                            ctctTTCAACGCCTCGACCAGGTCGGCATCGACCACCGCCACACCACGCCCGGCATTGATGAACAAGGCCGAGGGCTGGAAGCATTTGAACAGCGCGGCGTc                                                                                                      >  7:63382/1‑102 (MQ=255)
                                                                                               tttCAACGCCTCGACCAGGTCGGCATCGACCACCGCCACACCACGCCCGGCATTGATGAACAAGGCCGAGGGCTGGAAGCATTTGAACAGCGCGGCGTCATACAGGTCATGAGTCGCCGGGGTATCCGGCAGCAGGTTCAGCACGTAGt                                                    >  8:118974/1‑149 (MQ=255)
                                                                                                  cAACGCCTCGACCAGGTCGGCATCGACCACCGCCACACCACGCCCGGCATTGATGAACAAGGCCGAGGGCTGGAAGCATTTGAACAGCGCGGCGTCATACAGGTCATGAGTCGCCGGGGTATCCGGCAGCAGGTTCAGCACGTAGTCGa                                                 >  6:317144/1‑149 (MQ=255)
                                                                                                   aaCGCCTCGACCAGGTCGGCATCGACCACCGCCACACCACGCCCGGCATTTATGAACAAGGCCGAGGGCTGGAAGCATTTGAACAGCGCGGCGTCATACAGGTCATGAGTCGCCGGGGTATCCGGCAGCAGGTTCAGCACGTAGTCGa                                                 <  8:69180/148‑1 (MQ=255)
                                                                                                    aCGCCTCGACCAGGTCGGCATCGACCACCGCCACACCACGCCCGGCATTGATGAACAAGGCCGAGGGCTGGAAGCATTTGAACAGCGCGGCGTCATACAGGTCATGAGTCGCCGGGGTATCCGGCAGCAGGTTCAGCACGTAGTCGAcc                                               >  4:144396/1‑149 (MQ=255)
                                                                                                           gACCAGGTCGGCATCGACCACCGCCACACCACGCCCGGCATTGATGAACAAGGCCGAGGGCTGGAAGCATTTGAACAGCGCGGCGTCATACAGGTCATGAGTCGCCGGGGTATCCGGCAGCAGGTTCAGCACGTAGTCGACCTGGccaa                                        >  1:255258/1‑149 (MQ=255)
                                                                                                            aCCAGGTCGGCATAGACCACCGCCACACCACGCCCGGCATTGATGAACAAGGCCGAGGGCTGGAAGCATTTGAACAGCGCGGCGTCATACAGGTCATGAGTCGCCGGGGTATCCGGCAGCAGGTTCAGCACGTAGTCGACCTGGccaac                                       >  7:195086/1‑149 (MQ=255)
                                                                                                             ccAGGTCGGCATCGACCACCGCCACACCACGCCCGGCATTGATGAACAAGGCCGAGGGCTGGAAGCATTTGAACAGcgcg                                                                                                           <  1:246155/80‑1 (MQ=255)
                                                                                                             ccAGGTCGGCATCGACCACCGCCACACCACGCCCGGCATTGATGAACAAGGCCGAGGGCTGGAAGCATTTGAACAGcgcg                                                                                                           >  2:246155/1‑80 (MQ=255)
                                                                                                             ccAGGTCGGCATCGACCACCGCCACACCACGCCCGGCATTGATGAACAAGGCCGAGGGCTGGAAGCATTTGAACAGCGCGGCGTCATACAGGTCATGAGTCGCCGGGGTATCCGGCAGCAGGTTCAGCACGTAGTCGACCTGGccaacc                                      <  5:29503/149‑1 (MQ=255)
                                                                                                                    ggCATCGACCACCGCCACACCACGCCCGGCATTGATGAACAAGGCCGAGGGCTGGAAGCATTTGAACAGCGCGGCGTCATACAGGTCATGAGTCGCCGGGGTATCCGGCAGCAGGTTCAGCACGTAGTCGACCTGGCCAACCAAgcgc                                <  7:118974/148‑1 (MQ=255)
                                                                                                                     gCATCGACCACCGCCACACCACGCCCGGCATTGATGAACAAGGCCGAGGGCTGGAAGCATTAGAACAGCGCGGCGTCATACAGGTCAGGAGTCGCCGGGGTATCCGGCAGCAGGGTCAGCACGTAGTCGACCTGGCCACCAAAGcgcgc                              >  5:283121/1‑148 (MQ=255)
                                                                                                                       aTCGACCACCGCCACACCACGCCCGGCATTGATGAACAAGGCCGAGGGCTGGAAGCATTTGAACAGCGCGGCGTCATACAGGTCATGAGTCGCCGGGGTATCCGGCAGCAGGTTCAGCACGTAGTCGACCTGGCCAACCAAGCGCgg                              >  8:74102/1‑147 (MQ=255)
                                                                                                                          gACCACCGCCACACCACGCCCGGCATTGATGAACAAGGCCGAGGGCTGGAAGCATTTGAACAGCGCGGCGTCATACAGGTCATGAGTCGCCGGGGTATCCGGCAGCAGGTTCAGCACGTAGTCGACCTGGCCAACCAAGCGCGGCAgg                          <  6:112458/148‑1 (MQ=255)
                                                                                                                          gACCACCGCCACACCACGCCAGGCATTGATGAACAAGGCCGAGGGCTGGAAGCATTTGAACAGCGCGGCGTCATACAGGTCATGAGTCGCCGGGGTATCCGGCAGCAGGTTCAGCACGTAGTCGACCTGGCCAACCAAGCGCGGCAGGt                         >  6:251854/1‑149 (MQ=255)
                                                                                                                           accaccGCCACACCACGCCCGGCATTGATGAACAAGGCCGAGGGCTGGAAGCATTTGAACAGCGCGGCGTCATACAGGTCATGAGTCGCCGGGGTATCCGGCAGCAGGTTCAGCACGTAGTCGACCTGGCCAACCAAGCGCGGCAGGTc                        >  2:298060/1‑149 (MQ=255)
                                                                                                                            ccaccGCCACACCACGCCCGGCATTGATGAACAAGGCCGAGGGCTGGAAGCATTTGAACAGCGCGGCGTCATACAGGTCATGAGTCGCCGGGGTATCCGGCAGCAGGTTCAGCACGTAGTCGACCTGGCCAACCAAGCGCGGCAGGTCg                       >  4:245421/1‑149 (MQ=255)
                                                                                                                            ccaccGCCACACCACGCCCGGCATTGATGAACAAGGCCGAGGGCTGGAAGCATTTGAACAGCGCGGCGTCATACAGGTCATGAGTCGCCGGGGTATCCGGCAGCAGGTTCAGCACGTAGTCGACCTGGCCAACCAAGCGCGGCAGGTCg                       <  3:197246/149‑1 (MQ=255)
                                                                                                                              accGCCACACCACGCCCGGCATTGATGAACAAGGCCGAGGGCTGGAAGCATTTGAACAGCGCGGCGTCATACAGGTCATGAGTCGCCGGGGTATCCGGCAGCAGGTTCAGCACGTAGTCGACCTGGCCAACCAAGCGCGGCAGGTCGGc                     <  1:62262/149‑1 (MQ=255)
                                                                                                                               ccGCCACACCACGCCCGGCATTGATGAACAAGGCCGAGGGCTGGAAGCATTTGAACAGCGCGGCGTCATACAGGTCATGAGTCGCCGGGGTATCCGGCAGCAGGTTCAGCACGTAGTCGACCTGGCCAACCAAGCGCGGCAGGTCGGcc                    >  7:374585/1‑149 (MQ=255)
                                                                                                                                 gCCACACCACGCCCGGCATTGATGAACAAGGCCGAGGGCTGGAAGCATTTGAACAGCGCGGCGTCATACAGGTCATGAGTCGCCGGGGTATCCGGCAGCAGGTTCAGCACGTAGTCGACCTGGCCAACCAAGCGCGGCAGGTCGGCCAg                  >  5:225347/1‑149 (MQ=255)
                                                                                                                                     caccacGCCCGGCATTGATGAACAAGGCCGAGGGCTGGAAGCATTTGAACAGCGCGGCGTCATACAGGTCATGAGTCGCCGGGGTATCCGGCAGCAGGTTCAGCACGTAGTCGACCTGGCCAACCAAGCGCGGCAGGTCGGCCAGcgcc              <  7:226868/149‑1 (MQ=255)
                                                                                                                                       ccacGCCCGGCATTGATGAACAAGGCCGAGGGCTGGAAGCATTTGAACAGCGCGGCGTCATACAGGTCATGAGTCGCCGGGGTATCCGGCAGCAGGTTCAGCACGTAGTCGACCTGGCCAACCAAGCGCGGCAGGTCGGCCAGcgccgc            <  7:237349/149‑1 (MQ=255)
                                                                                                                                       ccacGCCCGGCATTGAAGAACAAGGCCGAGGGCTGGAAGCATTTGAACAGCGCGGCGTCATACAGCTCATGAGTCGCCGGGGTATCCGGCAGCAGGTTCAGCACGTAGTCGACCTGGCCAACCAAGCGCGGCAGGTCGGCCAGcgccgc            <  5:52050/149‑1 (MQ=255)
                                                                                                                                         acGCCCGGCATTGATGAACAAGGCCGAGGGCTGGAAGCATTTGAACAGCGCGGCGTCATACAGGTCATGAGTCGCCGGGGTATCCGGCAGCAGGTTCAGCACGTAGTCGACCTGGCCAACCAAGCGCGGCAGGTCGGCCAGCGCCGCCa          <  5:317144/149‑1 (MQ=255)
                                                                                                                                          cGCCCGGCATTGATGAACAAGGCCGAGGGCTGGAAGCATTTGAACAGCGCGGCGTCATACAGGTCATGAGTCGCCGGGGTATCCGGCAGCAGGTTCAGCACGTAGTCGACCTGGCCAACCAAGCGCGGCAGGTCGGCCAGCGCCGCCAc         <  8:375517/149‑1 (MQ=255)
                                                                                                                                             ccGGCATTGCTGCACAAGGCCGAGGGCTGGAAGCATTTGAACAGCGCGGCGTCATACAGGTCATGAGTCGCCGGGGTATCCGGCAGCAGGTTCAGCACGTAGTCGACCTGGCCAACCAAGCGCGGCAGGTCGGCCAGCGCCGCCACCTc      <  4:330063/149‑1 (MQ=255)
                                                                                                                                              cGGCATTGATGAACAAGGCCGAGGGCTGGAAGCATTTGAACAGCGCGGCGTCATACAGGTCATGAGTCGCCGGGGTATCCGGCAGCAGGTTCAGCACGTAGTCCACCTGGCCAACCAAGCGCGGCAGGTCGGCCAGCGCCGCCACCTcg     <  1:286449/149‑1 (MQ=255)
                                                                                                                                               ggCATTGATGAACAAGGCCGAGGGCTGGAAGCATTTGAACAGCGCGGCGTCATACAGGTCATGAGTCGCCGGGGTATCCGGCAGCAGGTTCAGCACGTAGTCGACCTGGCCAACCAAGCGCGGCAGGTCGGCCAGCGCCGCCACCTcga    <  6:225347/149‑1 (MQ=255)
                                                                                                                                                  aTTGATGAACAAGGCCGAGGGCTGGAAGCATTTGAACAGCGCGGCGTCATACAGGTCATGAGTCGCCGGGGTATCCGGCAGCAGGTTCAGCACGTAGTCGACCTGGCCAACCAAGCGCGGCAGGTCGGCCAGCGCCGCCACCTcgacg  >  5:419538/1‑148 (MQ=255)
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GTCAGCAGCAAGCCCCAGGCGGTCCAGAACGGGTGTTGCTTGGGCAACGGCTCCTGGCGGCACACATCGATCACAGCACCGGCCAGATGACCCTCTTTCAACGCCTCGACCAGGTCGGCATCGACCACCGCCACACCACGCCCGGCATTGATGAACAAGGCCGAGGGCTGGAAGCATTTGAACAGCGCGGCGTCATACAGGTCATGAGTCGCCGGGGTATCCGGCAGCAGGTTCAGCACGTAGTCGACCTGGCCAACCAAGCGCGGCAGGTCGGCCAGCGCCGCCACCTCGACG  >  NC_002947/5207897‑5208190

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑