| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,302,665 | T→C | 8.4% | S370G (AGC→GGC) | recB ← | Chi activated ATP‑dependent DNA helicase/dsDNA/ssDNA exonuclease |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,302,665 | 0 | T | C | 8.4% | 269.9 / 11.2 | 95 | S370G (AGC→GGC) | recB | Chi activated ATP‑dependent DNA helicase/dsDNA/ssDNA exonuclease |
| Reads supporting (aligned to +/- strand): ref base T (51/36); new base C (3/5); total (54/41) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.85e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.93e-01 | |||||||||||
ACAGACCGGTTTCTGCGCGATTTTCGCTGATCTGGTAGATGCGCTCGAAAATGCCGTACTGAACCGGGTC‑GGTGTCCTGGAATTCGTCGATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGC‑TCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCACGCCGGCGCTTCTCCACTTCGAAACGCGCCGATACCCAACTGGCGGCGTGCTCGAGCAGCGGGGCATCCGGGCTGTCCAGGGCCTGCAGTTGC > NC_002947/5302518‑5302810 | aCAGACCGGTTTCTGCGCGATTTTCGCTGATCTGGTAGATGCGCTCGAAAATGCCGTACTGAACCGGGTC‑GGTGTCCTGGAATTCGTCGATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGC‑TCGCCGGCCTCGCTg > 2:72038/1‑149 (MQ=255) aCCGGTTTCTGCGCGATTTTCGCTGATCTGGTAGATGCGCTCGAAAATGCCGTACTGAACCGGGTC‑GGTGTCCTGGAATTCGTCGATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGC‑TCGCCGGCCTCGCTGGc > 7:171194/1‑147 (MQ=255) aCCGGTTTCTGCGCGATTTTCGCTGATCTGGTAGATGCGCTCGAAAATGCCGTACTGAACCGGGTC‑GGTGTCCTGGAATTCGTCGATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGC‑TCGCCGGCCTCGCTGGc > 3:9224/1‑147 (MQ=255) ccGGTTTCTGCGCGATTTTCGCTGATCTGGTAGATGCGCTCGAAAATGCCGTACTGAACCGGGTC‑GGTGTCCTGGAATTCGTCGATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCACGCGC‑TCGCCAGCGAGGCCGGCGAg < 2:343150/149‑1 (MQ=255) cGGTTTCTGCGCGATTTTCGCTGATCTGGTAGATGCGCTCGAAAATGCCGTACTGAACCGGGTC‑GGTGTCCTGGAATTCGTCGATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGC‑TCGCCGGCCTCGCTGGCGAgc < 2:313399/149‑1 (MQ=255) cGGTTTCTGCGCGATTTTCGCTGATCTGGTAGATGCGCTCGAAAATGCCGTACTGAACCGGGTC‑GGTGTCCTGGAATTCGTCGATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCACGCGC‑TCGCCAGCGAGGCCGGCGAgc < 7:64906/149‑1 (MQ=255) gTTTCTGCGCGATTTTCGCTGATCTGGTAGATGCGCTCGAAAATGCCGTACTGAACCGGGTC‑GGTGTCCTGGAATTCGTCGATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGC‑TCGCCGGCCTCGCTGGCGAgcgc < 6:248740/149‑1 (MQ=255) tCTGCGCGATTTTCGCTGATCTGGTAGATGCGCTCGAAAATGCCGTACTGAACCGGGTC‑GGTGTCCTGGAATTCGTCGATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCACGCGC‑TCGCCAGCGAGGCCGGCGAgcgcct < 8:4907/148‑3 (MQ=255) tCTGCGCGATTTTCGCTGATCTGGTAGATGCGCTCGAAAATGCCGTACTGAACCGGGTC‑GGTGTCCTGGAATTCGTCGATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCACGCGC‑TCGCCAGCGAGGCCGGCGAgcgcct < 1:367993/148‑3 (MQ=255) gcgcGATTTTCGCTGATCTGGTAGATGCGCTCGAAAATGCCGTACTGAACCGGGTC‑GGTGTCCTGGAATTCGTCGATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGC‑TCGCCAGCGAGGCCGGCGAgcgcct < 5:222273/145‑3 (MQ=255) gcgcGATTTTCGCTGATCTGGTAGATGCGCTCGAAAATGCCGTACTGAACCGGGTC‑GGTGTCCTGGAATTCGTCGATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGC‑TCGCCAGCGAGGCCGGCGAgcgcct > 6:222273/1‑143 (MQ=255) cgcgATTTTCGCTGATCTGGTAGATGCGCTCGAAAATGCCGTACTGAACCGGGTC‑GGTGTCCTGGAATTCGTCGATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGC‑TCGCCGGCCTCGCTGGCGAGCGCGtgctg > 2:109220/1‑148 (MQ=255) cgcgATTTTCGCTGATCTGGTAGATGCGCTCGAAAATGCAGTACTGAACCGGGTC‑GGTGTCCTGGAATTCGTCGATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGC‑TCGCCGGCCTCGCTGGCGAGCGCGtgctg > 7:258391/1‑148 (MQ=255) cgATTTTCGCTGATCTGGTAGATGCGCTCGAAAATGCCGTACTGAACCGGGTC‑GGTGTCCTGGAATTCGTCGATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGC‑TCGCCGGCCTCGCTg < 6:339350/132‑1 (MQ=255) cgATTTTCGCTGATCTGGTAGATGCGCTCGAAAATGCCGTACTGAACCGGGTC‑GGTGTCCTGGAATTCGTCGATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGC‑TCGCCGGCCTCGCTg > 5:339350/1‑132 (MQ=255) ttttCGCTGATCTGGTAGATGCGCTCGAAAATGCCGTACTGAACCGGGTC‑GGTGTCCTGGAATTCGTCGATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGC‑TCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAgc < 4:9224/148‑1 (MQ=255) tttCGCTGATCTGGTAGATGCGCTCGAAAATGCCGTACTGAACCGGGTC‑GGTGTCCTGGAATTCGTCGATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGC‑TCGCCGGCCTCGCTGGCGCGCGCGTGCTGCAAgcg > 4:244002/1‑148 (MQ=255) tttCGCTGATCTGGTAGATGCGCTCGAAAATGCCGTACTGAACCGGGTC‑GGTGTCCTGGAATTCGTCGATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGC‑TCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAgcg < 2:349122/148‑1 (MQ=255) gCTGATCTGGTAGATGCGCTCGAAAATGGCGTACTGAACCGGGTCAGGTGTCCTGGAATTCGTCGATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGC‑TCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCAc > 1:287690/1‑148 (MQ=255) cttgTAGATGCGCTCGAAAATGCCGTACTGAACCTGGTC‑GGTTTCCTGTAATTCGTCGATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCTAGGCGC‑TCGCCTGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGAATg < 8:30520/145‑1 (MQ=255) tGGTAGATGCGCTCGAAAATGCCGTACTGAACCGGGTC‑GGTGTCCTGGAATTCGTCGATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGC‑TCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATgtc > 1:229894/1‑149 (MQ=255) tGGTAGATGCGCTCGAAAATGCCGTACTGAACCGGGTC‑GGTGTCCTGGAATTCGTCGATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGC‑TCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATgtc > 3:139120/1‑149 (MQ=255) gTAGATGCGCTCGAAAATGCTGTACTGAACCGGGTC‑GGTGTCCTGGAATTCGTCGATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGC‑TCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATgtc < 8:258391/147‑1 (MQ=255) gTAGATGCGCTCGAAAATGCCGTACTGAACCGGGTC‑GGTGTCCTGGAATTCGTCGATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGC‑TCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCAt < 8:171194/144‑1 (MQ=255) gTAGATGCGCTCGAAAATGCCGTACTGAACCGGGTC‑GGTGTCCTGGAATTCGTCGATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGC‑TCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATgtcgt > 5:357823/1‑149 (MQ=255) gTAGATGCGCTCGAAAATGCCGTACTGAACCGGGTC‑GGTGTCCTGGAATTCGTCGATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGC‑TCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATgtc < 1:109220/147‑1 (MQ=255) aTGCGCTCGAAAATGCCGTACTGAACCGGGTC‑GGTGTCCTGGAATTCGTCGATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGC‑TCGCCGGCCTCGCTGGCGa > 3:191858/1‑115 (MQ=255) aTGCGCTCGAAAATGCCGTACTGAACCGGGTC‑GGTGTCCTGGAATTCGTCGATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGC‑TCGCCGGCCTCGCTGGCGa < 4:191858/115‑1 (MQ=255) tCGAAAATGCCGTACTGAACCGGGTC‑GGTGTCCTGGAATTCGTCGATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGC‑TCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCAt > 3:194727/1‑149 (MQ=255) tCGAAAATGCCGTACTGAACCGGGTC‑GGTGTCCTGGAATTCGTCGATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGC‑TCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCAt > 5:201037/1‑149 (MQ=255) tCGAAAATGCCGTACTGAACCGGGTC‑GGTGTCCTGGAATTCGTCGATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGC‑TCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCAt > 5:347609/1‑149 (MQ=255) gAAAATGCCGTACTGAACCGGGTC‑GGTGTCCTGGAATTCGTCGATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGC‑TCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCAt < 4:145860/147‑1 (MQ=255) aaTGCCGTACTGAACCGGGTC‑GGTGTCCTGGAATTCGTCGATCAGCGCTACCGGGAACTGCTCACGGATCATGCTGGCCAGGCGCTTCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCt < 1:324431/149‑1 (MQ=255) atcccGTACTCAACCGGGTC‑GGTGTCCTGGAATTCGTCGATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGC‑TCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGc < 3:294206/146‑1 (MQ=255) gCCGTACTGAACCGGGTC‑GGTGTCCTGGAATTCGTCGATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGC‑TCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCa < 4:194727/148‑1 (MQ=255) gTACTGAACCGGGTC‑GGTGTCCGGGAATTCGTCGATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGC‑TCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCACGcc > 4:231716/1‑149 (MQ=255) tGAACCGGGTC‑GGTGTCCTGGAATTCGTCGATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCACGCGC‑TCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCACGCCGgcg > 2:212853/1‑149 (MQ=255) aaCCGGGTC‑GGTGTCCTGGAATTCGTCGATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGC‑TCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCACGCCGGCGCt > 7:161831/1‑149 (MQ=255) gTC‑GGTGTCCTGGAATTCGTCGATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGC‑TCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCACGCCGGCGCTTCTCCa > 3:259578/1‑149 (MQ=255) gtgtCCTGGAATTCGTCGATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGC‑TCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCACGCCGGCGCTTCTCCACTTc > 7:285465/1‑149 (MQ=255) gtgtCCTGGAATTCGTCGATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGC‑TCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCACGCCGGCGCTTCTCCACTTc < 1:72038/149‑1 (MQ=255) gtgtCCTGGAATTCGTCGAACAGCGATACCGGGAACTGCTCACCCATCAGGCTGGCCAGGCGC‑TCGCCGGAAACGCTTGCGAGCGCGTGCTGCAAGCGCACAAGCATGACGACGAAGCCAATTTCTGCACGCCGGCGCTTCTCCAcat > 4:401692/1‑146 (MQ=255) gtCCTGGAATTCGTCGATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGC‑TCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCACGCCGGCGCTTCTCCACTTCg > 6:333589/1‑148 (MQ=255) tGGAATTCGTCGATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGC‑TCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCACGCCGGCGCTTCTCCACTTCGAAAcg > 7:323269/1‑149 (MQ=255) tGGAATTCGTCGATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGC‑TCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCACGCCGGCGCTTCTCCACTTCGAAAcg > 3:35721/1‑149 (MQ=255) tGGAATTCGTCGATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGC‑TCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGAACGCCGGCGCTTCTCCACTTCGAAAcg > 1:147252/1‑149 (MQ=255) gAATTCGTCGATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGC‑TCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCACGCCGGCGCTTCTCCACTTCGAAAcgc < 4:35721/148‑1 (MQ=255) aaTTCGTCGATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGC‑TCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCACGCCGGCGCTTCTCCACTTCGAAACgcgc < 1:212853/149‑1 (MQ=255) aaTTCGTCGATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGC‑TCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCACGCCGGCGCTTCTCCACTTCGAAACgcgc < 6:347609/149‑1 (MQ=255) gtcgATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGC‑TCGCCGGCCTCGCTGGCGAGCGCGtgct < 6:203828/77‑1 (MQ=255) gtcgATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGC‑TCGCCGGCCTCGCTGGCGAGCGCGtgct > 5:203828/1‑77 (MQ=255) gtcgATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGC‑TCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCACGCCGGCGCTTCTCCACTTCGAAACGCGCCGATa > 1:123073/1‑149 (MQ=255) gtcgATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGC‑TCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCACGACGGCGCTTCTCCACTTCGAAACGCGCCGATa > 2:348557/1‑149 (MQ=255) cgATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGC‑TCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCACGCCGGCGCTtctc > 8:3286/1‑127 (MQ=255) cgATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGC‑TCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCACGCCGGCGCTtctc < 7:3286/127‑1 (MQ=255) gATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGC‑TCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCACGCCGGCGCTTCTCCACTTCGAAACGCGCCGATAcc > 5:359927/1‑148 (MQ=255) tCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGC‑TCGCCGGCCTCGCTGGCGAGCGCGtgc > 3:287976/1‑71 (MQ=255) tCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGC‑TCGCCGGCCTCGCTGGCGAGCGCGtgc < 4:287976/71‑1 (MQ=255) tCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGC‑TCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCACGCCGGCGCTTCTCCACTTCGAAACGCGCCGATACCCaa > 6:374818/1‑149 (MQ=255) gcgcTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGC‑TCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCACGCCGGCGCTTCTCCACTTCGAAACGCGCCGATACCCAACTg < 3:231716/149‑1 (MQ=255) cgcTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGC‑TCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCTAAGCCCATTTCTGCACGCCGGCGCTTCTCCACTTCGAAACGCGCCGATACCCAACTgg < 2:229894/149‑1 (MQ=255) cTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGC‑TCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCACGCCGGCGCTTCTCCACTTCGAAACGCGCCGATACCCAACTggcg < 2:287690/149‑1 (MQ=255) ccGGGAACTGCTCACGGATCAGGCTGGCCAGGCGC‑TCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCACGCCGGCGCTTCTCCACTTCGAAACGCGCCGATACCCAACt < 6:180023/142‑1 (MQ=255) ccGGGAACTGCTCACGGATCAGGCTGGCCAGGCGC‑TCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCACGCCGGCGCTTCTCCACTTCGAAACGCGCCGATACCCAACt > 5:180023/1‑142 (MQ=255) ccGGGAACTGCTCACGGATCAGGCTGGCCAGGCGC‑TCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCACGCCGGCGCTTCTCCACTTCGAAACGCGCCGATACCCAACTGgcggcg < 8:161831/149‑1 (MQ=255) cGGGAACTGCTCACGGATCAGGCTGGCCAGGCGC‑TCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCACGCCGGCGCTTCTCCACTTCGAAACGCGCCGATACCCCACTGGCGGCGt > 2:226204/1‑149 (MQ=255) gggAACTGCTCACGGATCAGGCTGGCCAGGCGC‑TCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCACGCCGGCGCTTCTCCACTTCGAAACGCGCCGATACCCAACTGGCGGCGTg > 2:2655/1‑149 (MQ=255) ggAACTGCTCACGGATCAGGCTGGCCAGGCGC‑TCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCACGCCGGCGCTTCTCCACTTCGAAACGCGCCGATACCCAACTGGCGGCGTGc > 7:12131/1‑149 (MQ=255) ggAACTGCTCACGGATCAGGCTGGCCAGGCGC‑TCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCACGCCGGCGCTTCTCCACTTCGAAACGCGCCGATACCCAACTGGCGGCGTGc > 1:366218/1‑149 (MQ=255) gAACTGCTCACGGATCAGGCTGGCCAGGCGC‑TCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATgt > 8:307806/1‑77 (MQ=255) gAACTGCTCACGGATCAGGCTGGCCAGGCGC‑TCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATgt < 7:307806/77‑1 (MQ=255) cTCACGGATCAGGCTGGCCAGGCGC‑TCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCACGCCGGCGCTTCTCCACTTCGAAACGCGCCGATACCCAACTGGCGGCGTGCTCGag > 4:321653/1‑147 (MQ=255) tCACGGATCAGGCTGGCCAGGCGC‑TCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCACGCCGGCGCTTCTCCACTTCGAAACGCGCCGATACCCAACTGGCGGCGTGCTc < 7:250602/143‑1 (MQ=255) tCACGGATCAGGCTGGCCAGGCGC‑TCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCACGCCGGCGCTTCTCCACTTCGAAACGCGCCGATACCCAACTGGCGGCGTGCTc > 8:250602/1‑143 (MQ=255) tCACGGATCAGGCTGGCCAGGCGC‑TCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCACGCCGGCGCTTCTCCACTTCGAAACGCGCCGATACCCAACTGGCGGCGTGCTCGagcag > 3:105910/1‑149 (MQ=255) tCACGGATCAGGCTGGCCAGGCGC‑TCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCACGCCGGCGCTTCTCCACTTCGAAACGCGCCGATACCCAACTGGCGGCGTGCTCGagcag > 7:311971/1‑149 (MQ=255) aCGGATCAGGCTGGCCAGGCGC‑TCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCACGCCGGCGCTTCTCCACTTCGAAACGCGCCGATACCCAACTGGCGGCGTGCTCGAGCAGCg > 6:172838/1‑149 (MQ=255) ggATCAGGCTGGCCAGGCGC‑TCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCACGCCGGCGCTTCTCCACTTCGAAACGCGCCGATACCCAACTGGCGGCGTGCTCGAGCAGCggg < 2:123073/149‑1 (MQ=255) cAGGCTGGCCAGGCGC‑TCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCACGCCGGCGCTTCTCCACTTCGAAACGCGCCGATACCCAACTGGCGGCGTGCTCGAGCAGCGGGGCAt > 3:309396/1‑149 (MQ=255) aGGCTGGCCAGGCGC‑TCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCACGCCGGCGCTTCTCCACTTCGAAACGCGCCGATACCCAACTGGCGGCGTGCTCGAGCAGCGGGGCAt < 2:366218/148‑1 (MQ=255) ggCTGGCCAGGCGC‑TCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCCCGCCGGCGCTTCTCCACTTCGAAACGCGCCGATACCCAACTGGCGGCGTGCTCGAGCAGCGGGGCATcc > 7:82564/1‑149 (MQ=255) gCTGGCCAGGCGC‑TCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCACGCCGGCGCTTCTCCACTTCGAAACGCGCCGATACCCAACTGGCGGCGTGCTCGAGCAGCGGGGCATCCg > 6:418031/1‑149 (MQ=255) gCTGGCCAGGCGC‑TCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCACGCCGGCGCTTCTCCACTTCGAAACGCGCCGATACCCAACTGGCGGCGTGCTCGAGCAGCGGGGCATCCg > 6:298608/1‑149 (MQ=255) cTGGCCAGGCGC‑TCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCACGCCGGCGCTTCTCCACTTCGAAACGCGCCGATACCCAACTGGCGGCGTGCTCGAGCAGCGGGGCATCCgg < 6:359927/149‑1 (MQ=255) ccAGGCGC‑TCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCACGCCGGCGCTTCTCCACTTCGAAACGCGCCGATACCCAACTGGCGGCGTGCTCGAGCAGCGGGGCATCCGGGCTg > 5:199756/1‑149 (MQ=255) acgcgc‑TCGCCAGCGAGGCCGGCGAGCGCGTGCTGCAAGCGCGCGAGCATGTAGTCGAAGCCCATTTCTGCACGCCGGCGCTTCTCCACTTCGAAACGCGCCGATACCCAACTGGAGGCGTGCTCGAGCAGCGGGGCATCCGGGCTggc > 5:109332/3‑147 (MQ=255) ggCGC‑TCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCACGCCGGCGCTTCTCCACTTCGAAACGCGCCGATACCCAACTGGCGGCGTGCTCGAGCAGCGGGGCATCCGGGCTGTcc < 6:357823/149‑1 (MQ=255) cgcgc‑TCGCCAGCGAGGCCGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCACGCCGGCGCTTCTCCACTTCGAAACGCGCCGATACCCAACTGGCGGCGTGCTCGCGCAGCGGGGCATCCGGGCTGTc > 7:342736/2‑148 (MQ=255) gcgc‑TCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCACGCCGGCGCTTCTCCACTTCGAAACGCGCCGATACCCAACTGGCGGCGTGCTCGAGCAGCGGGGCATCCGGGCTGTCCa < 3:225376/149‑1 (MQ=255) c‑TCGCCGGCCTCGCTGGCGAGCGCGTTCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCACGCCGGCGCTTCTCCACTTCGAAACGCGCCGATACCCAACTGGCGGCGTGCTCGAGCAGCGGGGCATCCGGGCTGTCCAggg < 8:12131/149‑1 (MQ=255) tCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCCCGCCGGCGCTTCTCCACTTCGAAACGCGCCGATACCCAACTGGCGGCGTGCTCGAGCAGCGGGGCATCCGGGCTGTCCAGGGc > 7:143746/1‑149 (MQ=255) ccGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCACGCCGGCGCTTCTCCACTTCGAAACGCGCCGATACCCAACTGGCGGCGTGCTCGAGCAGCGGGGCATCCGGGCTGTCCAGGGCCTg > 8:194882/1‑149 (MQ=255) cTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCACGCCGGCGCTTCTCCACTTCGAAACGCGCCGATACCCAACTGGCGGCGTGCTCGAGCAGCGGGGCATCCGGGCTg > 5:390958/1‑133 (MQ=255) cTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCACGCCGGCGCTTCTCCACTTCGAAACGCGCCGATACCCAACTGGCGGCGTGCTCGAGCAGCGGGGCATCCGGGCTg < 6:390958/133‑1 (MQ=255) gCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCACGCCGGCGCTTCTCCACTTCGAAACGCGCCGATACCCAACTGGCGGCGTGCTCGAGCAGCGGGGCATCCGGGCTGTCCAGGGCCTGCAgattc < 4:107718/148‑5 (MQ=255) | ACAGACCGGTTTCTGCGCGATTTTCGCTGATCTGGTAGATGCGCTCGAAAATGCCGTACTGAACCGGGTC‑GGTGTCCTGGAATTCGTCGATCAGCGCTACCGGGAACTGCTCACGGATCAGGCTGGCCAGGCGC‑TCGCCGGCCTCGCTGGCGAGCGCGTGCTGCAAGCGCACGAGCATGTCGTCGAAGCCCATTTCTGCACGCCGGCGCTTCTCCACTTCGAAACGCGCCGATACCCAACTGGCGGCGTGCTCGAGCAGCGGGGCATCCGGGCTGTCCAGGGCCTGCAGTTGC > NC_002947/5302518‑5302810 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |