Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,322,154 C→T 6.1% S366S (TCG→TCA)  mrcB ← transglycosylase/transpeptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,322,1540CT 6.1% 197.3 / 2.1 65S366S (TCG→TCA) mrcBtransglycosylase/transpeptidase
Reads supporting (aligned to +/- strand):  ref base C (32/29);  new base T (2/2);  total (34/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01

GCTTGAAGGTCTCGCTCATCGAGGTTTCGGCCTTCATCTGCAGGATAGGGTCGAAGCTGGTAAAGATGCGCAGGCCTTCTTCGGTCAAGTCCTCGTCGCGGTAATCCTGGCGCAACTGTCGCTTGACCAGGTCGAGGAAGGCTGGGAACGAGCTGTCGGCCAGGCTGCCACGCTTGGTCACGCCCAGCGGCATTTTCTTCGCCGCATCGACTTCTGCCTGGCTGGCCACGCCCTGCTCGGCCACCAGGTCGAGCACCAGGTTGCGGCGAGCCAGGGCCCGCTCGGGGTGACGCCGCG  >  NC_002947/5322006‑5322302
                                                                                                                                                    |                                                                                                                                                    
gcTTGAAGGTCTCGCTCATCGAGGTTTCGGCCTTCATCTGCAGGATAGGGTCGAAGCTGGTAAAGATGCGCCGGCCTTCTTCGGTCTAGTCCTCGTCGCGGTATTGCTGGCGCAACTGTCGCTTGACCAGGTCGAGGAAGGCTGGGAAc                                                                                                                                                      <  4:333686/149‑1 (MQ=255)
  ttGAAGGTCTCGCTCATCGAGGTTTCGGCCTTCATCTGCAGGATAGGGTCGAAGCTGGTAAAGATGCGCAGGCCTTCTTCGGTCAAGTCCTCGTCGCGGTAATCCTGGCGCAACTGTCGCTTGACCAGGTCGAGGAAGGCTGGGAAc                                                                                                                                                      >  8:44581/1‑147 (MQ=255)
    gAAGGTCTCGCTCATCGAGGTTTCGGCCTTCATCTGCAGGATAGGGTCGAAGCTGGTAAAGATGCGCAGGCCTTCTTCGGTCAAGTCCTCGTCGCGGTAATCCTGGCGCAACTGTCGCTTGACCAGGTCGAGGAAGGCTGGGAACGAGc                                                                                                                                                  >  1:316444/1‑149 (MQ=255)
          ctcGCTCATCGAGGTTTCGGCCTTCATCTGCAGGATAGGGTCGAAGCTGGTAAAGATGCGCAGGCCTTCTTCGGTCAAGTCCTCGTCGCGGTAATCCTGGCGCAACTGTCGCTTGACCAGGTCGAGGAAGGCTGGGAACGAGCTGTCgg                                                                                                                                            >  3:65710/1‑149 (MQ=255)
           tcGCTCATCGAGGTTTCGGCCTTCATCTGCAGGATAGGGTCGAAGCTGGTAAAGATGCGCAGGCCTTCTTCGGTCAAGTCCTCGTCGCGGTAATCCTGGCGCAACTGTCGCTTGACCAGGTCGAGGAAGGCTGGGAACGAGCTGTCGGc                                                                                                                                           >  2:9392/1‑149 (MQ=255)
             gCTCATCGAGGTTTCGGCCTTCATCTGCAGGATAGGGTCGAAGCTGGTAAAGATGCGCAGGCCTTCTTCGGTCAAGTCCTCGTCGCGGTAATCCTGGCGCAACTGTCGCTTGACCAGGTCGAGGAAGGCTGGGAACGAGCTGTCGGc                                                                                                                                           >  3:274586/1‑147 (MQ=255)
                 aTCGAGGTTTCGGCCTTCATCTGCAGGATAGGGTCGAAGCTGGTAAAGATGCGCAGGCCTTCTTCGGTCAAGTCCTCGTCGCGGTAATCCTGGCGCAACTGTCGCTTGACCAGGTCGAGGAAGGCTGGGAACGAGCTGTCGGCCAgg                                                                                                                                       <  8:32813/147‑1 (MQ=255)
                  tCGAGGTTTCGGCCTTCATCTGCAGGATAGGGTCGAAGCTGGTAAAGATGCGCAGGCCTTCTTCGGTCAAGTCCTCGTCGCGGTAATCCTGGCGCAACTGTCGCTTGACCAGGTCGAGGAAGGCTGGGAACGAGCTGTCGGCCAGGCTg                                                                                                                                    >  1:303838/1‑149 (MQ=255)
                   cGAGGTTTCGGCCTTCATCTGCAGGATAGGGTCGAAGCTGGTAAAGATGCGCAGGCCTTCTTCGGTCAAGTCCTCGTCGCGGTAATCCTGGCGCAACTGTCGCTTGACCAGGTCGAGGAAGGCTGGGAACGAGCTGTCGGCCAGGCTGc                                                                                                                                   >  2:179732/1‑149 (MQ=255)
                    gAGGTTTCGGCCTTCATCTGCAGGATAGGGTCGAAGCTGGTAAAGATGCGCAGGCCTTCTTCGGTCAAGTCCTCGTCGCGGTAATCCTGGCGCAACTGTCGCTTGACCAGGTCGAGGAAGGCTGGGAACGAGCTGTCGGCCAGGCTGc                                                                                                                                   <  4:99504/148‑1 (MQ=255)
                     aGGTTTCGGCCTTCATCTGCAGGATAGGGTCGAAGCTGGTAAAGATGCGCAGGCCTTCTTCGGTCAAGTCCTCGTCGCGGTAATCCTGGCGCAACTGTCGCTTGACCAGGTCGAGGAAGGCTGGGAACGAGCTGTCGGCCAGGCTGCCa                                                                                                                                 >  3:353135/1‑149 (MQ=255)
                           cGGCCTTCATCTGCAGGATAGGGTCGAAGCTGGTAAAGATGCGCAGGCCTTCTTCGGTCAAGTCCTCGTCGCGGTAATCCTGGCGCAACTGTCGCTTGACCAGGTCGAGGAAGGCTGGGAACGAGCTGTCGGCCAGGCTGCCACGCtt                                                                                                                            <  2:303838/148‑1 (MQ=255)
                            ggCCTTCATCTGCAGGATAGGGTCGAAGCTGGTAAAGATGCGCAGGCCTTCTTCGGTCAAGTCCTCGTCGCGGTAATCCTGGCGCAACTGTCGCTTGACCAGGTCGAGGAAGGCTGGGAACGAGCTGTCGGCCAGGCTGCCACGCTTgg                                                                                                                          <  1:9392/149‑1 (MQ=255)
                            ggCCTTCATCTGCAGGATAGGGTCGAAGCTGGTAAAGATGCGCAGGCCTTCTTCGGTCAAGTCCTCGTCGCGGTAATCCTGGCGCAACTGTCGCTTGACCAGGTCGAGGAAGGCTGGGAACGAGCTGTCGGCCAGGCTGCCACGCTTgg                                                                                                                          <  3:122355/149‑1 (MQ=255)
                            ggCCTTCATCTGCAGGATAGGGTCGAAGCTGGTAAAGATGCGCAGGCCTTCTTCGGTCAAGTCCTCGTCGCGGTAATCCTGGCGCAACTGTCGCTTGACCAGGTCGAGGAAGGCTGGGAACGAGCTGTCGGCCAGGCTGCCACGCTTg                                                                                                                           <  2:206342/148‑1 (MQ=255)
                                   aTCTGCAGGATAGGGTCGAAGCTGGTAAAGATGCGCAGGCCTTCTTCGGTCAAGTCCTCGTCGCGGTAATCCTGGCGCAACTGTCGCTTGACCAGGTCGAGGAAGGCTGGGAACGAGCTGTCGGCCAGGCTGCCACGCTTGGTCACg                                                                                                                     <  2:225775/147‑1 (MQ=255)
                                   aTCTGCAGGATAGGGTCGAAGCTGGTAAAGATGCGCAGGCCTTCTTCGGTCAAGTCCTCGTCGCGGTAATCCTGGCGCAACTGTCGCTTGACCAGGTCGAGGAAGGCTGGGAACGAGCTGTCGGCCAGGCTGCCACGCTTGGTCACg                                                                                                                     >  1:225775/1‑147 (MQ=255)
                                        cAGGATAGGGTCGAAGCTGGTAAAGATGCGCAGGCCTTCTTCGGTCAAGTCCTCGTCGCGGTAATCCTGGCGCAACTGTCGCTTGACCAGGTCGAGGAAGGCTGGGAACGAGCTGTCGGCCAGGCTGCCACGCTTGGTCACGCCCa                                                                                                                 <  1:39215/146‑1 (MQ=255)
                                             tAGGGTCGAAGCTGGTAAAGATGCGCAGGCCTTCTTCGGTCAAGTCCTCGTCGCGGTAATCCTGGCGCAACTGTCGCTTGACCAGGTCGAGGAAGGCTGGGAACGAGCTGTCGGCCAGGCTGCCACGCTTGGTCACGCCCAGCGGCAtt                                                                                                         >  1:73923/1‑149 (MQ=255)
                                             tAGGGTCGAAGCTGGTAAAGATGCGCAGGCCTTCTTCGGTCAAGTCCTCGTCGCGGTAATCCTGGCGCAACTGTCGCTTGACCAGGTCGAGGAAGGCTGGGAACGAGCTGTCGGCCAGGCTGCCACGCTTGGTCACGCCCAGCGGCAtt                                                                                                         <  2:73923/149‑1 (MQ=255)
                                               gggTCGAAGCTGGTAAAGATGCGCAGGCCTTCTTCGGTCAAGTCCTCGTCGCGGTAATCCTGGCGCAACTGTCGCTTGACCAGGTCGAGGAAGGCTGGGAACGAGCTGTCGGCCAGGCTGCCACGCTTGGTCACGCCCAGCGGCAttt                                                                                                        <  1:45632/148‑1 (MQ=255)
                                                ggTCGAAGCTGGTAAAGATGCGCAGGCCTTCTTCGGTCAAGTCCTCGTCGCGGTAATCCTGGCGCAACTGTCGCTTGACCAGGTCGAGGAAGGCTGGGAACGAGCTGTCGGCCAGGCTGCCACGCTTGGTCACGCCCAGCGGCATTttc                                                                                                      >  8:193861/1‑149 (MQ=255)
                                                 gTCGAAGCTGGTAAAGATGCGCAGGCCTTCTTCGGTCAAGTCCTCGTCGCGGTAATCCTGGCGCAACTGTCGCTTGACCAGGTCGAGGAAGGCTGGGAACGAGCTGTCGGCCAGGCTGCCACGCTTGGTCACGCCCAGCGGCATTttct                                                                                                     >  8:347558/1‑149 (MQ=255)
                                                    gAAGCTGGTAAAGATGCGCAGGCCTTCTTCGGTCAAGTCCTCGTCGCGGTAATCCTGGCGCAACTGTCGCTTGACCAGGTCGAGGAAGGCTGGGAACGAGCTGTCGGCCAGGCTGCCACGCTTGGTCACGCCCAGCGGCATTttcttc                                                                                                   <  6:395616/148‑1 (MQ=255)
                                                    gAAGCTGGTAAAGATGCGCAGGCCTTCTTCGGTCAAGTCCTCGTCGCGGTAATCCTGGCGCAACTGTCGCTTGACCAGGTCGAGGAAGGCTGGGAACGAGCTGTCGGCCAGGCTGCCACGCTTGGTCACGCCCAGCGGCATTttcttc                                                                                                   <  4:274586/148‑1 (MQ=255)
                                                    gAAGCTGGTAAAGATGCGCAGGCCTTCTTCGGTCAAGTCCTCGTCGCGGTAATCCTGGCGCAACTGTCGCTTGACCAGGTCGAGGAAGGCTGGGAACGAGCTGTCGGCCAGGCTGCCACGCTTGGTCACGCCCAGCGGCATTttcttc                                                                                                   <  6:389874/148‑1 (MQ=255)
                                                    gAAGCTGGTAAAGATGCGCAGGCCTTCTTCGGTCAAGTCCTCGTCGCGGTAATCCTGGCGCAACTGTCGCTTGACCAGGTCGAGGAAGGCTGGGAACGAGCTGTCGGCCAGGCTGCCACGCTTGGTCACGCCCAGCGGCATTttcttc                                                                                                   <  1:206658/148‑1 (MQ=255)
                                                    gAAGCTGGTAAAGATGCGCAGGCCTTCTTCGGTCAAGTCCTCGTCGCGGTAATCCTGGCGCAACTGTCGCTTGACCAGGTCGAGGAAGGCTGGGAACGAGCTGTCGGCCAGGCTGCCACGCTTGGTCACGCCCAGCGGCATTTTCTTcg                                                                                                  >  3:30996/1‑149 (MQ=255)
                                                       gCTGGTAAAGATGCGCAGGCCTTCTTCGGTCAAGTCCTCGTCGCGGTAATCCTGGCGCAACTGTCGCTTGACCAGGTCGAGGAAGGCTGGGAACGAGCTGTCGGCCAGGCTGCCACGCTTGGTCACGCCCAGCGGCATTTTCTTcgccg                                                                                               <  4:65710/149‑1 (MQ=255)
                                                           gTAAAGATGCGCAGGCCTTCTTCGGTCAAGTCCTCGTCGCGGTAATCCTGGCGCAACTGTCGCTTGACCAGGTCGAGGAAGGCTGGGAACGAGCTGTCGGCCAGGCTGCCACGCTTGGTCACGCCCAGCGGTATTTTCTTCGCCGCAt                                                                                            <  4:201284/148‑1 (MQ=255)
                                                           gTAAAGATGCGCAGGCCTTCTTCGGTCAAGTCCTCGTCGCGGTAATCCTGGCGCAACTGTCGCTTGACCAGGTCGAGGAAGGCTGGGAACGAGCTGTCGGCCAGGCTGCCACGCTTGGTCACGCCCAGCGGCATTTTCTTCGCCGCATc                                                                                           >  4:181671/1‑149 (MQ=255)
                                                           gTAAAGATGCGCAGGCCTTCTTCGGTCAAGTCCTCGTCGCGGTAATCCTGGCGCAACTGTCGCTTGACCAGGTCGAGGAAGGCTGGGAACGAGCTGTCGGCCAGGCTGCCACGCTTGGTCACGCCCAGCGGCATTTTCTTCGCCGCATc                                                                                           >  4:175455/1‑149 (MQ=255)
                                                                  tGCGCAGGCCTTCTTCGGTCAAGTCCTCGTCGCGGTAATCCTGGCGCAACTGTCGCTTGACCAGGTCGAGGAAGGCTGGGAACGAGCTGTCGGCCAGGCTGCCACGCTTGGTCACGCCCAGCGGCATTTTCTTCGCCGCATCGACTTCt                                                                                    <  8:17554/149‑1 (MQ=255)
                                                                       aGGCCTTCTTCGGTCAAGTCCTGGTCGCGGTAATCCTGGCGCAAGTGTCGCTTGACCAGGTCGAGGAAGGCTGGGAACGAGGTGTCGGCCAGGCTGCCACGCTTGGTCACGCCCAGCGGCATTTTCTTCGCCGCATCGACTTCTGCctg                                                                               <  3:226461/149‑1 (MQ=255)
                                                                       aGGCCTTCTTCGGTCAAGTCCTCGTCGCGGTAATCCTGGCGCAACTGTCGCTTGCCCAGGTCGAGGAAGGCTGGGAACGAGCTGTCGGCCAGGCTGCCACGCATGGTCACGCCCAGCGGCATTTTATTCGCCGCATAGACTTCGGCctg                                                                               >  2:92869/1‑149 (MQ=255)
                                                                            ttcttcGGTCAAGTCCTCGTCGCGGTAATCCTGGCGCAACTGTCGCTTGACCAGGTCGAGGAAGGCTGGGAACGAGCTGTCGGCCAGGCTGCCACGCTTGGTCACGCCCAGCGGCATTTTCTTCGCCGCATCGACTTCTGCctggctgg                                                                          >  6:64913/1‑149 (MQ=255)
                                                                              cttcGGTCAAGTCCTCGTCGCGGTAATCCTGGCGCAACTGTCGCTTGACCAGGTCGAGGAAGGCTGGGAACGAGCTGTCGGCCAGGCTGCCACGCTTGGTCACGCCCAGCGGCATTTTCTTCGCCGCATCGACTTCTGCCtggctggc                                                                         <  5:64913/148‑1 (MQ=255)
                                                                                 cGGTCAAGTCCTCGTCGCGGTAATCCTGGCGCAACTGTCGCTTGACCAGGTCGAGGAAGGCTGGGAACGAGCTGTCGGCCAGGCTGCCACGCTTGGTCACGCCCAGCGGCATTTTCTTCGCCGCATCGACTTCTGCCTGGCTGGcc                                                                        <  4:353135/146‑1 (MQ=255)
                                                                                    tCAGGTCCTCGTCGCGGTAATCCTGGCGCAACTGTCGCTTGACCAGGTCGAGGAAGGCTGGGAACGAGCTGTCGGCCAGGCTGCCACGCTTGGTCACGCCCAGCGGCATTTTCTTcgccgc                                                                                              >  3:238381/1‑121 (MQ=255)
                                                                                    tCAAGTCCTCGTCGCGGTAATCCTGGCGCAACTGTCGCTTGACCAGGTCGAGGAAGGCTGGGAACGAGCTGTCGGCCAGGCTGCCACGCTTGGTCACGCCCAGCGGCATTTTCTTcgccgc                                                                                              <  4:238381/121‑1 (MQ=255)
                                                                                            tcgtagCGGTAAACCTGGCGCAACTCTCGCTTGACAAGGTCGAGGAAGGCAGGGAACGAGCTGTCCGCCAGGCTGCCACGCTTGGTCACACCCATCGGCATTTTCTTCTCCTCATAGACTTCTGCCTGCCTGGCCACGCCCTGcaccgc                                                          >  4:138434/1‑144 (MQ=255)
                                                                                                 gcgGTAATCCTGGCGCAACTGTCGCTTGACCAGGTCGAGGAAGGCTGGGAACGAGCTGTCGGCCAGGCTGCCACGCTTGGTCACGCCCAGCGGCATTTTCTTCGCCGCATCGACTTCTGCCTGGCTGGCCACGCCCTGCTCGGccacca                                                     <  7:347558/149‑1 (MQ=255)
                                                                                                          cTGGCGCAACTGTCGCTTGACCAGGTCGAGGAAGGCTGGGAACGAGCTGTCGGCCAGGCTGCCACGCTTGGTCACGCCCAGCGGCATTTTCTTCGCCGCATCGACTTCTGCCTGGCTGGCCACGCCCTGCTCGGCCACCAGGTCGAGc                                             <  4:30996/148‑1 (MQ=255)
                                                                                                             gcgcAACTGTCGCTTGACCAGGTCGAGGAAGGCTGGGAATGAGCTGTCGGCCAGGCTGCCACGCTTGGTCACGCCCAGCGGCATTTTCTTCGCCGCATCGACTTCTGCCTGGCTGGCCACGCCCTGCTCGGCCACCAGGTCGAGCAc                                           >  4:32455/1‑147 (MQ=255)
                                                                                                             gcgcAACTGTCGCTTGACCAGGTCGAGGAAGGCTGGGAATGAGCTGTCGGCCAGGCTGCCACGCTTGGTCACGCCCAGCGGCATTTTCTTCGCCGCATCGACTTCTGCCTGGCTGGCCACGCCCTGCTCGGCCACCAGGTCGAGCAc                                           >  4:27100/1‑147 (MQ=255)
                                                                                                             gcgcAACTGTCGCTTGACCAGGTCGAGGAAGGCTGGGAACGAGCTGTCGGCCAGGCTGCCACGCTTGGTCACGCCCAGCGGCATTTTCTTCGCCGCATCGACTTCTGCCTGGCTGGCCACGCCCTGCTCGGCCACCAGGTCGAGCAcc                                          <  7:44581/148‑1 (MQ=255)
                                                                                                                   cTGTCGCTTGACCAGGTCGAGGAAGGCTGGGAACGAGCTGTCGGCCAGGCTGCCACGCTTGGTCACGCCCAGCGGCATTTTCTTCGCCGCATCGACTTCTGCCTGGCTGGCCACGCCCTGCTCGGCCACCAGGTCGAGCACCAGGTTgc                                   >  7:258816/1‑149 (MQ=255)
                                                                                                                      tCGCTTGACCAGGTCGAGGAAGGCTGGGAATGAGCTGTCGGCCAGGCTGCCACGCTTGGTCACGCCCAGCGGCATTTTCTTCGCCGCATCGACTTCTGCCTGGCTGGCCACGCCCTGCTCGGCCACCAGGTCGAGCACCAGGTTgcg                                  <  3:27100/147‑1 (MQ=255)
                                                                                                                      tCGCTTGACCAGGTCGAGGAAGGCTGGGAATGAGCTGTCGGCCAGGCTGCCACGCTTGGTCACGCCCAGCGGCATTTTCTTCGCCGCATCGACTTCTGCCTGGCTGGCCACGCCCTGCTCGGCCACCAGGTCGAGCACCAGGTTgcg                                  <  3:32455/147‑1 (MQ=255)
                                                                                                                        gCTTGACCAGGTCGAGGAAGGCTGGGAACGAGCTGTCGGCCAGGCTGCCACGCTTGGTCACGCCCAGCGGCATTTTCTTCGCCGCATCGACTTCTGCCTGGCTGGCCACGCCCTGCTCGGCCACCAGGTCGAGCACCAGGTTgcggc                                >  8:64156/1‑147 (MQ=255)
                                                                                                                         cTTGACCAGGTCGATGAAGGCTGGGAACGAGCTGTCGGCCAGGCTGCCACGCTTGGTCACGCCCAGCGGCATTTTCTTCGCCGCATCGACTTCTGCCTGGCTGGCCACGCCCTGCTCGGCCACCAGGTCGAGCACCAGGTTGCGGCGAg                             >  5:228193/1‑149 (MQ=255)
                                                                                                                          ttGACCAGGTCGAGGAAGGCTGGGAACGAGCTGTCGGCCAGGCTGCCACGCTTGGTCACGCCCAGCGGCATTTTCTTCGCCGCATCGACTTCTGCCTGGCTGGCCACGCCCTGCTCGGCCACCAGGTCGAGCACCAGGTTGCGGCGAGc                            >  2:262494/1‑149 (MQ=255)
                                                                                                                              ccAGGTCGAGGAAGGCTGGGAACGAGCTGTCGGCCAGGCTGCCACGCTTGGTCACGCCCAGCGGCATTTTCTTCGCCGCATCGACTTCTGCCTGGCTGGCCACGCCCTGCTCGGCCACCAGGTCGAGCACCAGGTTGCGGCGAGCCTgg                        >  5:112034/1‑149 (MQ=255)
                                                                                                                              ccAGGTCGAGGAAGGCTGGGAACGAGCTGTCGGCCAGGCTGCCACGCTTGGTCACGCCCAGCGGCATTTTCTTCGCCGCATCGACTTCTGCCTGGCTGGCCACGCCCTGCTCGGCCACCAGGTCGAGCACCAGGTTGCGGCGAGCCAgg                        <  1:92869/149‑1 (MQ=255)
                                                                                                                                         aaGGCTGGGAACGAGCTGTCGGCCAGGCTGCCACGCTTGGTCACGCCCAGCGGCATTTTCTTCGCCGCATCGACTTCTGCCTGGCTGGCCACGCCCTGCTCGGCCACCAGGTCGAGCACCAGGTTGCGGCGAGCCAGGGCCCGCTCggg             <  7:55684/149‑1 (MQ=255)
                                                                                                                                           ggCTGGGAACGAGCTGTCGGCCAGGCTGCCACGCTTGGTCACGCCCAGCGGCATTTTCTTCGCCGCATCGACTTCTGCCTGGCTGGCCACGCCCTGCTCGGCCACCAGGTCGAGCACCAGGTTGCGGCGAGCCAGGGCCCGCTCGGGGt           >  5:397769/1‑149 (MQ=255)
                                                                                                                                           ggCTGGGAACGAGCTGTCGGCCAGGCTGCCACGCTTGGTCACGCCCAGCGGCATTTTCTTCGCCGCATCGACTTCTGCCTGGCTGGCCACGCCCTGCTCGGCCACCAGGTCGAGCACCAGGTTGCGGCGAGCCAGGGCCCGCTCGGGGt           <  5:362676/149‑1 (MQ=255)
                                                                                                                                           ggCTGGGAACGAGCGGGCGGCCAGGCTGCCACGCTTGGTCACGCCCAGCGGCATTTTCTTCGCCGCATCGACTTCTGCCTGGCTGGCCACGCCCTGCTCGGCCACCAGGTCGAGCACCAGGTTGCGGCGAGCCAGGGCCCGCTCGGGGt           <  2:249375/149‑1 (MQ=255)
                                                                                                                                             cTGGGAACGAGCTGTCGGCCAGGCTGCCACGCTTGGTCACGCCCAGCGGCATTTTCTTCGCCGCATCGACTTCTGCCTGGCTGGCCACGCCCTGCTCGGCCACCAGGTCGAGCACCAGGTTGCGGCGAGCCAGGGCCCGCTCGGGGTGa         >  5:215681/1‑149 (MQ=255)
                                                                                                                                             cTGGGAACGAGCTGTCGGCCAGGCTGCCACGCTTGGTCACGCCCAGCGGCATTTTCTTCGCCGCATCGACTTCTGCCTGGCTGGCCACGCCCTGCTCGGCCACCAGGTCGAGCACCAGGTTGCGGCGAGCCAGGGCCCGCTCGGGGTGa         >  5:221239/1‑149 (MQ=255)
                                                                                                                                             cTGGGAACGAGCTGTCGGCCAGGCTGCCACGCTTGGTCACGCCCAGCGGCATTTTCTTCGCCGCATCGACTTCTGCCTGGCTGGCCACGCCCTGCTCGGCCACCAGGTCGAGCACCAGGTTGCGGCGAGCCAGGGCCCGCTCGGGGTGa         >  1:169444/1‑149 (MQ=255)
                                                                                                                                             cTGGGAACCAGCTGTCCGCCAGCCTCCCACGCTACGACACGCCCAGCGCCAATTTCTTCGCCCCATCGACTTCAGCCTGGCAGGCCACCCCCTGCTCGGCCACCAGCTCGAGCACCACGTTGCGGCGAGCCAGGGCCCGCTcccggaga         >  2:238000/1‑142 (MQ=255)
                                                                                                                                              tGGGAACGAGCTGTCGGCCAGGCTGCCACGCTTGGTCACGCCCAGCGGCATTTTCTTCGCCGCATCGACTTCTGCCTGGCTGGCCACGCCCTGCTCGGCCACCAGGTCGAGCACCAGGTTGCGGCGAGCCAGGGCCCGCTCGGGGTGAc        >  8:308913/1‑149 (MQ=255)
                                                                                                                                                ggAACGAGCTGTCGGCCAGGCTGCCACGCTTGGTCACGCCCAGCGGCATTTTCTTCGCCGCATCGACTTCTGCCTGGCTGGCCACGCCCTGCTCGGCCACCAGGTCGAGCACCAGGTTGCGGCGAGCCAGGGCCCGCTCGGGGTGAc        <  1:238000/147‑1 (MQ=255)
                                                                                                                                                 gAACGAGCTGTCGGCCAGGCTGCCACGCTTGGTCACGCCCAGCGGCATTTTCTTCGCCGCATCGACTTCTGCCTGGCTGGCCACGCCCTGCTCGGCCACCAGGTCGAGCACCAGGTTGCGGCGAGCCAGGGCCCGCTCGGGGTGAcgcc     >  2:277487/1‑149 (MQ=255)
                                                                                                                                                 gAACGAGCTGTCGGCCAGGCTGCCACGCTTGGTCACGCCCAGCGGCATTTTCTTCGCCGCATCGACTTCTGCCTGGCTGGCCACGCCCTGCTCGGCCACCAGGTCGAGCACCAGGTTGCGGCGAGCCAGGGCCCGCTCGGGGTGAcgcc     >  8:194466/1‑149 (MQ=255)
                                                                                                                                                  aaCGAGCTGTCGGCCAGGCTGCCACGCTTGGTCACGCCCAGCGGCATTTTCTTCGCCGCATCGACTTCTGCCTGGCTGGCCACGCCCTGCTCGGCCACCAGGTCGAGCACCAGGTTGCGGCGAGCCAGGGCCCGCTCGGGGTGAcgccg    >  3:244245/1‑149 (MQ=255)
                                                                                                                                                    cGAGCTGTCGGCCAGGCTGCCACGCTTGGTCACGCCCAGCGGCATTTTCTTCGCCGCATCGACTTCTGCCTGGCTGGCCACGCCCTGCTCGGCCACCAGGTCGAGCACCAGGTTGCGGCGAGCCAGGGCCCGCTCGGGGTGACGCcgcg  <  1:277487/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GCTTGAAGGTCTCGCTCATCGAGGTTTCGGCCTTCATCTGCAGGATAGGGTCGAAGCTGGTAAAGATGCGCAGGCCTTCTTCGGTCAAGTCCTCGTCGCGGTAATCCTGGCGCAACTGTCGCTTGACCAGGTCGAGGAAGGCTGGGAACGAGCTGTCGGCCAGGCTGCCACGCTTGGTCACGCCCAGCGGCATTTTCTTCGCCGCATCGACTTCTGCCTGGCTGGCCACGCCCTGCTCGGCCACCAGGTCGAGCACCAGGTTGCGGCGAGCCAGGGCCCGCTCGGGGTGACGCCGCG  >  NC_002947/5322006‑5322302

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑