Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,339,889 C→T 6.1% L373L (CTG→TTG)  pcnB → poly(A) polymerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,339,8890CT 6.1% 248.0 / 3.2 82L373L (CTG→TTG) pcnBpoly(A) polymerase
Reads supporting (aligned to +/- strand):  ref base C (43/34);  new base T (2/3);  total (45/37)
Fisher's exact test for biased strand distribution p-value = 6.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.13e-01

TGTGCTGCACCTGCAGAACCAGGGCGTGCCGCCGATCCCGGCCATGAACGGTGCGGCCCACGACCTGATCGCCGAGCAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACCGACGAACTGGGCCAGTGGTGGACCGATTACCAGGACGCAAACGA  >  NC_002947/5339741‑5340037
                                                                                                                                                    |                                                                                                                                                    
tgtgCTGCACCTGCAGAACCAGGGCGTGCCGCCGATCCCGGCCATGAACGGTGCGGCCCACGACCTGATCGCCGAGCAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGcc                                                                                                                                                      <  6:241877/149‑1 (MQ=255)
  tgctgcACCTGCAGAACCAGGGCGTGCCGCCGATCCCGGCCATGAACGGTGCGGCCCACGACCTGATCGCCGAGCAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCt                                                                                                                                                     <  6:349697/148‑1 (MQ=255)
              aGAACCAGGGCGTGCCGCCGATCCCGGCCATGAACGGTGCGGCCCACGACCTGATCGCCGAGCAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGc                                                                                                                                        <  3:304634/149‑1 (MQ=255)
              aGAACCAGGGCGTGCCGCCGATCCCGGCCATGAACGGTGCGGCCCACGACCTGATCGCCGAGCAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGc                                                                                                                                        >  8:300294/1‑149 (MQ=255)
                 aCCAGGGCGTGCCGCCGATCCCGGCCATGAACGGTGCGGCCCACGACCTGATCGCCGAGCAGTGCGCGCGCATAGCCATCCCCGAGCGGATTACCCTGCAGATCCGCCAGAACTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGAgg                                                                                                                                      >  5:355164/1‑148 (MQ=255)
                   cAGGGCGTGCCGCCGATCCCGGCCATGAACGGTGCGGCCCACGACCTGATCGCCGAGCAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCTTGCCGCTGCGGCGCGGCag                                                                                                                                   <  1:276688/149‑2 (MQ=255)
                     gggCGTGCCGCCGATCCCGGCCATGAACGGTGCGGCCCACGACCTGATCGCCGAGCAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCTGCGGCGCGGCAGgc                                                                                                                                 <  3:291811/149‑1 (MQ=255)
                     gggCGTGCCGCCGATCCCGGCCATGAACGGTGCGGCCCACGACCTGATCGCCGAGCAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAgc                                                                                                                                 <  3:391619/149‑1 (MQ=255)
                     gggCGTGCCGCCGATCCCGGCCATGAACGGTGCGGCCCACGACCTGATCGCCGAGCAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAgc                                                                                                                                 >  2:113298/1‑149 (MQ=255)
                       gCGTGCCGCCGATCCCGGCCATGAACGGTGCGGCCCACGACCTGATCGCCGAGCAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAgcgc                                                                                                                               <  6:323636/149‑1 (MQ=255)
                         ggcccgccgATCCCGGCCATGAACGGTGCGGCCCACGACCTGATCGCCGAGCAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCTTGCCGCTGCGGCGCGGCAGgcgctc                                                                                                                             <  1:274297/146‑3 (MQ=255)
                                cgATCCCGGCCATGAACGGTGCGGCCCACGACCTGATCGCCGAGCAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACAtg                                                                                                                      <  3:79670/149‑1 (MQ=255)
                                     ccGGCCATGAACGGTGCGGCCCACGACCTGATCGCCGAGCAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATgctgct                                                                                                                 >  6:152627/1‑149 (MQ=255)
                                     ccGGCCATGAACGGTGCGGCCCAAGACCTGATCGCCGAGCAGTGCGCGAGCATTGCCATCCCAAAGCGTTTTACACTGCCGATCCGCGAGATCTGTGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACAtgctgc                                                                                                                  <  2:273672/148‑1 (MQ=255)
                                      cGGCCATGAACGGTGCGGCCCACGACCTGATCGCCGAGCAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTgccgcgccg                                                                                                                                            <  1:220788/121‑1 (MQ=255)
                                      cGGCCATGAACGGTGCGGCCCACGACCTGATCGCCGAGCAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTgccgcgccg                                                                                                                                            >  2:220788/1‑121 (MQ=255)
                                      cGGCCATGAACGGTGCGGCCCACGACCTGATCGCCGAGCAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGctgctg                                                                                                                >  5:358052/1‑149 (MQ=255)
                                       ggCCATGAACGGTGCGGCCCACGACCTGATCGCCGAGCAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGAGCCGCAGCGGCAAGCGCGCCGACATGctgctg                                                                                                                >  4:332054/1‑148 (MQ=255)
                                           aTGAACGGTGCGGCCCACGACCTGATCGCCGAGCAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACAtgctgc                                                                                                                  <  6:412723/142‑1 (MQ=255)
                                           aTGAACGGTGCGGCCCACGACCTGATCGCCGAGCAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGAGCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACAtgctgc                                                                                                                  >  5:412723/1‑142 (MQ=255)
                                             gAACGGTGCGGCCCACGACCTGATCGCCGAGCAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAAcc                                                                                                         >  8:259407/1‑149 (MQ=255)
                                             gAACGGTGCGGCCCACGACCTGATCGCCGAGCAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCATGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCAGAAGGACAAcc                                                                                                         >  8:375100/1‑149 (MQ=255)
                                                   tGCGGCCCACGACCTGATCGCCGAGCAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGtt                                                                                                   >  7:362609/1‑149 (MQ=255)
                                                          cACGACCTGATCGCCGAGCAGTGCGCGCGCATTGCCATCCCCACGCGTTTTACCCTGCCTATCCGCGAGATCTGGGACATGCATGAGCGCCTGCCTCGCCGCAGCGGCAAGCGCGCCGAAATGCTTCTGGACAACCCGCGTTTCCgcgc                                                                                            <  8:369232/149‑1 (MQ=255)
                                                             gACCTGATCGCCGAGCAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCgg                                                                                         >  5:143428/1‑149 (MQ=255)
                                                             gACCTGATCGCCGAGCAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCgg                                                                                         >  1:361336/1‑149 (MQ=255)
                                                                cTGATCGCCGAGCAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTa                                                                                      <  2:267468/149‑1 (MQ=255)
                                                                  gATCGCCGAGCAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTAc                                                                                     >  2:357032/1‑148 (MQ=255)
                                                                   aTCGCCGAGCAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTAc                                                                                     <  1:113298/147‑1 (MQ=255)
                                                                   aTCGCCGAGCAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGa                                                                                   >  5:128797/1‑149 (MQ=255)
                                                                       ccGAGCAGTGCGCGCGCATTGCCATCCCCAAGCGATTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTc                                                                               >  1:246503/1‑149 (MQ=255)
                                                                        cGAGCAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTcc                                                                              <  8:362609/149‑1 (MQ=255)
                                                                         gAGCAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCct                                                                             <  3:332054/149‑1 (MQ=255)
                                                                         gAGCAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCct                                                                             >  7:145798/1‑149 (MQ=255)
                                                                           gCAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCctgc                                                                           >  3:273955/1‑149 (MQ=255)
                                                                            cAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCTCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGTACAACCCTCGTTTCCGCGCCGGTTACGACTTCctgct                                                                          <  7:375100/149‑1 (MQ=255)
                                                                               tGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCctgctgct                                                                       <  1:357032/149‑1 (MQ=255)
                                                                                 cgcgcgcATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCtgctgctgc                                                                     <  7:300294/149‑1 (MQ=255)
                                                                                    gcgcATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGt                                                                   >  6:363738/1‑148 (MQ=255)
                                                                                      gcATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGaa                                                                <  6:38158/149‑1 (MQ=255)
                                                                                        aTTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAg                                                              >  3:368274/1‑149 (MQ=255)
                                                                                         ttGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAgc                                                             >  4:347618/1‑149 (MQ=255)
                                                                                           gCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAgcgc                                                           >  8:200464/1‑149 (MQ=255)
                                                                                            ccATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGtt                                                                                       <  2:298663/120‑1 (MQ=255)
                                                                                            ccATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAAGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGtt                                                                                       >  1:298663/1‑120 (MQ=255)
                                                                                               tCCCCAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCggg                                                       <  6:128797/149‑1 (MQ=255)
                                                                                                 cccAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCgggg                                                      >  7:204437/1‑148 (MQ=255)
                                                                                                  ccAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACACCCCGAGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGCAAGCGCCgggg                                                      >  7:339245/1‑147 (MQ=255)
                                                                                                   cAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCcgc                                                                                              >  8:251288/1‑106 (MQ=255)
                                                                                                    aaGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGgaaga                                                  <  5:48689/149‑1 (MQ=255)
                                                                                                         ttttACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAAcc                                              >  5:163779/1‑148 (MQ=255)
                                                                                                         ttttACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACcg                                             <  2:109284/149‑1 (MQ=255)
                                                                                                             aCCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGTCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCgg                                                        >  6:155704/1‑134 (MQ=255)
                                                                                                             aCCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGTCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCgg                                                        <  5:155704/134‑1 (MQ=255)
                                                                                                             aCCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCgg                                                        <  3:223039/134‑1 (MQ=255)
                                                                                                             aCCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCgg                                                        >  4:223039/1‑134 (MQ=255)
                                                                                                             aCCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACcgacg                                          >  8:182477/1‑148 (MQ=255)
                                                                                                              cccTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCtgctgctgc                                                                     >  3:218063/1‑120 (MQ=255)
                                                                                                              cccTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCtgctgctgc                                                                     <  4:218063/120‑1 (MQ=255)
                                                                                                                 tGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACCGACGAACTg                                     >  3:292152/1‑149 (MQ=255)
                                                                                                                   ccGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACCGACGAACTggg                                   >  6:219711/1‑149 (MQ=255)
                                                                                                                   ccGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACCGACGAACTgg                                    <  5:152627/148‑1 (MQ=255)
                                                                                                                       tCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACCGACGAACTGGGCCAg                               >  5:346233/1‑149 (MQ=255)
                                                                                                                        ccGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGCAATGCTTCTGGACAACCCTCGTTTCCGAGCCGGTTACGACTTACTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACCGAAGAACTGGGCCact                              >  3:33157/1‑147 (MQ=255)
                                                                                                                         cgcgAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACCGACGAACTGGGCCAgtg                             >  1:345609/1‑149 (MQ=255)
                                                                                                                          gcgAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACCGCCGAACTGGGCCAgtgg                            >  8:377966/1‑149 (MQ=255)
                                                                                                                          gcgAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACCGACGAACTGGGCCAgtgg                            >  3:102141/1‑149 (MQ=255)
                                                                                                                           cgAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACCGACGAACTGGGCCAgtggt                           <  4:102141/149‑1 (MQ=255)
                                                                                                                             agaTCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACCGACGAACTGGGCCAGtggtgg                         <  7:259407/149‑1 (MQ=255)
                                                                                                                              gaTCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACCGACGAACTGGGCCAGTGGTGGa                        >  6:335147/1‑149 (MQ=255)
                                                                                                                              gaTCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGG‑TACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACCGACGAACTGGGCCAgtggtg                          >  1:200223/1‑146 (MQ=255)
                                                                                                                              gaTCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGG‑TACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACCGACGAACTGGGCCAgtggtg                          <  2:200223/146‑1 (MQ=255)
                                                                                                                                    ggACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACCGACGAACTGGGCCAGTGGTGGACCGAt                   <  5:335147/148‑1 (MQ=255)
                                                                                                                                      aCATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACCGACGAACTGGGCCAgtggtg                          <  8:238593/139‑1 (MQ=255)
                                                                                                                                      aCATGAAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACCGACGAACTGGGCCAgtggtg                          >  7:238593/1‑139 (MQ=255)
                                                                                                                                       cATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACCGACGAACTGGGCCAGTGGTGGACCGATTAcc               <  4:273955/149‑1 (MQ=255)
                                                                                                                                         tGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACCGACGAACTGGGCCAGTGGTGGACCGATTACCAg             <  4:292152/149‑1 (MQ=255)
                                                                                                                                          gCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGTAGAAACCGACGAACTGGGCCAGTGGTGGACCGATTACCAgg            >  5:351593/1‑149 (MQ=255)
                                                                                                                                            aGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACCGACGAACTGGGCCAGTGGTGGACCGATTACCAGCAc          >  8:108783/1‑149 (MQ=255)
                                                                                                                                             ggcgcgcTTGCCGCTGCGGCGCGGCAGGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTTCGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACCGACGAACTGGGCCAGTGGTGGACCGATTACCAGGACg         >  2:127065/4‑149 (MQ=255)
                                                                                                                                              gcgcgcTTGCCGCTGCGGCGCGGCAGGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACCGACGAACTGGGCCAGTGGTGGACCGATTa                 >  2:33462/3‑140 (MQ=255)
                                                                                                                                              gcgcgcTTGCCGCTGCGGCGCGGCAGGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACCGACGAACTGGGCCAGTGGTGGACCGATTa                 <  1:33462/138‑1 (MQ=255)
                                                                                                                                                  gcCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACCGACGAACTGGGCCAGTGGTGGACCGATTACCAGGACGCAAAc    <  2:361336/149‑1 (MQ=255)
                                                                                                                                                    cTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACCGACGAACTGGGCCAGTGGTGGACCGATTACCAGGACGCAAACGa  >  4:136173/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
TGTGCTGCACCTGCAGAACCAGGGCGTGCCGCCGATCCCGGCCATGAACGGTGCGGCCCACGACCTGATCGCCGAGCAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACCGACGAACTGGGCCAGTGGTGGACCGATTACCAGGACGCAAACGA  >  NC_002947/5339741‑5340037

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑