Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,339,900 C→G 7.4% R376R (CGC→CGG)  pcnB → poly(A) polymerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,339,9000CG 7.4% 247.5 / 8.6 81R376R (CGC→CGG) pcnBpoly(A) polymerase
Reads supporting (aligned to +/- strand):  ref base C (44/31);  new base G (2/4);  total (46/35)
Fisher's exact test for biased strand distribution p-value = 3.95e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.91e-01

AGAACCAGGGCGTGCCGCCGATCCCGGCCATGAACGGTGCGGCCCACGACCTGATCGCCGAGCAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACCGACGAACTGGGCCAGTGGTGGACCGATTACCAGGACGCAAACGACAGCGAGCGCC  >  NC_002947/5339755‑5340048
                                                                                                                                                 |                                                                                                                                                    
aGAACCAGGGCGTGCCGCCGATCCCGGCCATGAACGGTGCGGCCCACGACCTGATCGCCGAGCAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGc                                                                                                                                                   <  3:304634/149‑1 (MQ=255)
aGAACCAGGGCGTGCCGCCGATCCCGGCCATGAACGGTGCGGCCCACGACCTGATCGCCGAGCAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGc                                                                                                                                                   >  8:300294/1‑149 (MQ=255)
   aCCAGGGCGTGCCGCCGATCCCGGCCATGAACGGTGCGGCCCACGACCTGATCGCCGAGCAGTGCGCGCGCATAGCCATCCCCGAGCGGATTACCCTGCAGATCCGCCAGAACTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGAgg                                                                                                                                                 >  5:355164/1‑148 (MQ=255)
     cAGGGCGTGCCGCCGATCCCGGCCATGAACGGTGCGGCCCACGACCTGATCGCCGAGCAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCTTGCCGCTGCGGCGCGGCag                                                                                                                                              <  1:276688/149‑2 (MQ=255)
       gggCGTGCCGCCGATCCCGGCCATGAACGGTGCGGCCCACGACCTGATCGCCGAGCAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCTGCGGCGCGGCAGgc                                                                                                                                            <  3:291811/149‑1 (MQ=255)
       gggCGTGCCGCCGATCCCGGCCATGAACGGTGCGGCCCACGACCTGATCGCCGAGCAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAgc                                                                                                                                            <  3:391619/149‑1 (MQ=255)
       gggCGTGCCGCCGATCCCGGCCATGAACGGTGCGGCCCACGACCTGATCGCCGAGCAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAgc                                                                                                                                            >  2:113298/1‑149 (MQ=255)
         gCGTGCCGCCGATCCCGGCCATGAACGGTGCGGCCCACGACCTGATCGCCGAGCAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAgcgc                                                                                                                                          <  6:323636/149‑1 (MQ=255)
           ggcccgccgATCCCGGCCATGAACGGTGCGGCCCACGACCTGATCGCCGAGCAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCTTGCCGCTGCGGCGCGGCAGgcgctc                                                                                                                                        <  1:274297/146‑3 (MQ=255)
                  cgATCCCGGCCATGAACGGTGCGGCCCACGACCTGATCGCCGAGCAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACAtg                                                                                                                                 <  3:79670/149‑1 (MQ=255)
                       ccGGCCATGAACGGTGCGGCCCACGACCTGATCGCCGAGCAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATgctgct                                                                                                                            >  6:152627/1‑149 (MQ=255)
                       ccGGCCATGAACGGTGCGGCCCAAGACCTGATCGCCGAGCAGTGCGCGAGCATTGCCATCCCAAAGCGTTTTACACTGCCGATCCGCGAGATCTGTGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACAtgctgc                                                                                                                             <  2:273672/148‑1 (MQ=255)
                        cGGCCATGAACGGTGCGGCCCACGACCTGATCGCCGAGCAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGctgctg                                                                                                                           >  5:358052/1‑149 (MQ=255)
                         ggCCATGAACGGTGCGGCCCACGACCTGATCGCCGAGCAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGAGCCGCAGCGGCAAGCGCGCCGACATGctgctg                                                                                                                           >  4:332054/1‑148 (MQ=255)
                             aTGAACGGTGCGGCCCACGACCTGATCGCCGAGCAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACAtgctgc                                                                                                                             <  6:412723/142‑1 (MQ=255)
                             aTGAACGGTGCGGCCCACGACCTGATCGCCGAGCAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGAGCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACAtgctgc                                                                                                                             >  5:412723/1‑142 (MQ=255)
                               gAACGGTGCGGCCCACGACCTGATCGCCGAGCAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAAcc                                                                                                                    >  8:259407/1‑149 (MQ=255)
                               gAACGGTGCGGCCCACGACCTGATCGCCGAGCAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCATGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCAGAAGGACAAcc                                                                                                                    >  8:375100/1‑149 (MQ=255)
                                     tGCGGCCCACGACCTGATCGCCGAGCAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGtt                                                                                                              >  7:362609/1‑149 (MQ=255)
                                            cACGACCTGATCGCCGAGCAGTGCGCGCGCATTGCCATCCCCACGCGTTTTACCCTGCCTATCCGCGAGATCTGGGACATGCATGAGCGCCTGCCTCGCCGCAGCGGCAAGCGCGCCGAAATGCTTCTGGACAACCCGCGTTTCCgcgc                                                                                                       <  8:369232/149‑1 (MQ=255)
                                               gACCTGATCGCCGAGCAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCgg                                                                                                    >  1:361336/1‑149 (MQ=255)
                                               gACCTGATCGCCGAGCAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCgg                                                                                                    >  5:143428/1‑149 (MQ=255)
                                                  cTGATCGCCGAGCAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTa                                                                                                 <  2:267468/149‑1 (MQ=255)
                                                    gATCGCCGAGCAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTAc                                                                                                >  2:357032/1‑148 (MQ=255)
                                                     aTCGCCGAGCAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTAc                                                                                                <  1:113298/147‑1 (MQ=255)
                                                     aTCGCCGAGCAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGa                                                                                              >  5:128797/1‑149 (MQ=255)
                                                         ccGAGCAGTGCGCGCGCATTGCCATCCCCAAGCGATTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTc                                                                                          >  1:246503/1‑149 (MQ=255)
                                                          cGAGCAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTcc                                                                                         <  8:362609/149‑1 (MQ=255)
                                                           gAGCAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCct                                                                                        <  3:332054/149‑1 (MQ=255)
                                                           gAGCAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCct                                                                                        >  7:145798/1‑149 (MQ=255)
                                                             gCAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCctgc                                                                                      >  3:273955/1‑149 (MQ=255)
                                                              cAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCTCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGTACAACCCTCGTTTCCGCGCCGGTTACGACTTCctgct                                                                                     <  7:375100/149‑1 (MQ=255)
                                                                 tGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCctgctgct                                                                                  <  1:357032/149‑1 (MQ=255)
                                                                   cgcgcgcATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCtgctgctgc                                                                                <  7:300294/149‑1 (MQ=255)
                                                                      gcgcATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGt                                                                              >  6:363738/1‑148 (MQ=255)
                                                                        gcATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGaa                                                                           <  6:38158/149‑1 (MQ=255)
                                                                          aTTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAg                                                                         >  3:368274/1‑149 (MQ=255)
                                                                           ttGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAgc                                                                        >  4:347618/1‑149 (MQ=255)
                                                                             gCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAgcgc                                                                      >  8:200464/1‑149 (MQ=255)
                                                                              ccATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGtt                                                                                                  <  2:298663/120‑1 (MQ=255)
                                                                              ccATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAAGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGtt                                                                                                  >  1:298663/1‑120 (MQ=255)
                                                                                 tCCCCAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCggg                                                                  <  6:128797/149‑1 (MQ=255)
                                                                                   cccAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCgggg                                                                 >  7:204437/1‑148 (MQ=255)
                                                                                    ccAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACACCCCGAGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGCAAGCGCCgggg                                                                 >  7:339245/1‑147 (MQ=255)
                                                                                     cAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCcgc                                                                                                         >  8:251288/1‑106 (MQ=255)
                                                                                      aaGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGgaaga                                                             <  5:48689/149‑1 (MQ=255)
                                                                                           ttttACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAAcc                                                         >  5:163779/1‑148 (MQ=255)
                                                                                           ttttACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACcg                                                        <  2:109284/149‑1 (MQ=255)
                                                                                               aCCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGTCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCgg                                                                   <  5:155704/134‑1 (MQ=255)
                                                                                               aCCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGTCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCgg                                                                   >  6:155704/1‑134 (MQ=255)
                                                                                               aCCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCgg                                                                   >  4:223039/1‑134 (MQ=255)
                                                                                               aCCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCgg                                                                   <  3:223039/134‑1 (MQ=255)
                                                                                               aCCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACcgacg                                                     >  8:182477/1‑148 (MQ=255)
                                                                                                cccTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCtgctgctgc                                                                                <  4:218063/120‑1 (MQ=255)
                                                                                                cccTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCtgctgctgc                                                                                >  3:218063/1‑120 (MQ=255)
                                                                                                   tGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACCGACGAACTg                                                >  3:292152/1‑149 (MQ=255)
                                                                                                     ccGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACCGACGAACTggg                                              >  6:219711/1‑149 (MQ=255)
                                                                                                     ccGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACCGACGAACTgg                                               <  5:152627/148‑1 (MQ=255)
                                                                                                         tCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACCGACGAACTGGGCCAg                                          >  5:346233/1‑149 (MQ=255)
                                                                                                          ccGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGCAATGCTTCTGGACAACCCTCGTTTCCGAGCCGGTTACGACTTACTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACCGAAGAACTGGGCCact                                         >  3:33157/1‑147 (MQ=255)
                                                                                                           cgcgAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACCGACGAACTGGGCCAgtg                                        >  1:345609/1‑149 (MQ=255)
                                                                                                            gcgAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACCGCCGAACTGGGCCAgtgg                                       >  8:377966/1‑149 (MQ=255)
                                                                                                            gcgAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACCGACGAACTGGGCCAgtgg                                       >  3:102141/1‑149 (MQ=255)
                                                                                                             cgAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACCGACGAACTGGGCCAgtggt                                      <  4:102141/149‑1 (MQ=255)
                                                                                                               agaTCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACCGACGAACTGGGCCAGtggtgg                                    <  7:259407/149‑1 (MQ=255)
                                                                                                                gaTCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACCGACGAACTGGGCCAGTGGTGGa                                   >  6:335147/1‑149 (MQ=255)
                                                                                                                gaTCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGG‑TACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACCGACGAACTGGGCCAgtggtg                                     >  1:200223/1‑146 (MQ=255)
                                                                                                                gaTCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGG‑TACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACCGACGAACTGGGCCAgtggtg                                     <  2:200223/146‑1 (MQ=255)
                                                                                                                      ggACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACCGACGAACTGGGCCAGTGGTGGACCGAt                              <  5:335147/148‑1 (MQ=255)
                                                                                                                        aCATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACCGACGAACTGGGCCAgtggtg                                     <  8:238593/139‑1 (MQ=255)
                                                                                                                        aCATGAAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACCGACGAACTGGGCCAgtggtg                                     >  7:238593/1‑139 (MQ=255)
                                                                                                                         cATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACCGACGAACTGGGCCAGTGGTGGACCGATTAcc                          <  4:273955/149‑1 (MQ=255)
                                                                                                                           tGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACCGACGAACTGGGCCAGTGGTGGACCGATTACCAg                        <  4:292152/149‑1 (MQ=255)
                                                                                                                            gCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGTAGAAACCGACGAACTGGGCCAGTGGTGGACCGATTACCAgg                       >  5:351593/1‑149 (MQ=255)
                                                                                                                              aGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACCGACGAACTGGGCCAGTGGTGGACCGATTACCAGCAc                     >  8:108783/1‑149 (MQ=255)
                                                                                                                               ggcgcgcTTGCCGCTGCGGCGCGGCAGGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTTCGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACCGACGAACTGGGCCAGTGGTGGACCGATTACCAGGACg                    >  2:127065/4‑149 (MQ=255)
                                                                                                                                gcgcgcTTGCCGCTGCGGCGCGGCAGGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACCGACGAACTGGGCCAGTGGTGGACCGATTa                            >  2:33462/3‑140 (MQ=255)
                                                                                                                                gcgcgcTTGCCGCTGCGGCGCGGCAGGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACCGACGAACTGGGCCAGTGGTGGACCGATTa                            <  1:33462/138‑1 (MQ=255)
                                                                                                                                    gcCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACCGACGAACTGGGCCAGTGGTGGACCGATTACCAGGACGCAAAc               <  2:361336/149‑1 (MQ=255)
                                                                                                                                      cTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACCGACGAACTGGGCCAGTGGTGGACCGATTACCAGGACGCAAACGa             >  4:136173/1‑149 (MQ=255)
                                                                                                                                       tGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAGCCCGCGTTTCCGCGCCGGTTATGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACCGACGAACTGGGCCAGTGGTGGACCGATTACCAGGACGCAAACGa             >  3:89347/1‑148 (MQ=255)
                                                                                                                                          cgcgccgcAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACCGACGAACTGGGCCAGTGGTGGACCGATTACCAGGACGCAAACGACagc         <  6:346233/149‑1 (MQ=255)
                                                                                                                                           gcgccgcAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACCGACGAACTGGGCCAGTGGTGGACCGATTACCAGGACGCAAACGACagc         >  2:314603/1‑148 (MQ=255)
                                                                                                                                            cgccgcAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACCGACGAACTGGGCCAGTGGTGGACCGATTACCAGGACGCAAACGACagcga       <  2:345609/149‑1 (MQ=255)
                                                                                                                                                gcAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACCGACGAACTGGGCCAGTGGTGGACCGATTACCAGGACGCAAACGACAGCGAgcgc   >  2:184404/1‑149 (MQ=255)
                                                                                                                                                 cAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACCGACGAACTGGGCCTGTGGTGGACCGATTACCAGGACGCAAACGACAGCGAGcgcc  >  5:352187/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
AGAACCAGGGCGTGCCGCCGATCCCGGCCATGAACGGTGCGGCCCACGACCTGATCGCCGAGCAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACCGACGAACTGGGCCAGTGGTGGACCGATTACCAGGACGCAAACGACAGCGAGCGCC  >  NC_002947/5339755‑5340048

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑