Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,339,908	A→G	5.8%	K379R (AAG→AGG)	pcnB →	poly(A) polymerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,339,908	0	A	G	5.8%	259.7 / 4.6	86	K379R (AAG→AGG)	pcnB	poly(A) polymerase
Reads supporting (aligned to +/- strand):  ref base A (48/33);  new base G (2/3);  total (50/36)
Fisher's exact test for biased strand distribution p-value = 6.45e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.55e-01

GGGCGTGCCGCCGATCCCGGCCATGAACGGTGCGGCCCACGACCTGATCGCCGAGCAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACCGACGAACTGGGCCAGTGGTGGACCGATTACCAGGACGCAAACGACAGCGAGCGCCGCGAG  >  NC_002947/5339762‑5340053                                                                                                                                                   |                                                                                                                                                  gggCGTGCCGCCGATCCCGGCCATGAACGGTGCGGCCCACGACCTGATCGCCGAGCAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCTGCGGCGCGGCAGgc                                                                                                                                                 <  3:291811/149‑1 (MQ=255) gggCGTGCCGCCGATCCCGGCCATGAACGGTGCGGCCCACGACCTGATCGCCGAGCAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAgc                                                                                                                                                 <  3:391619/149‑1 (MQ=255) gggCGTGCCGCCGATCCCGGCCATGAACGGTGCGGCCCACGACCTGATCGCCGAGCAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAgc                                                                                                                                                 >  2:113298/1‑149 (MQ=255)   gCGTGCCGCCGATCCCGGCCATGAACGGTGCGGCCCACGACCTGATCGCCGAGCAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAgcgc                                                                                                                                               <  6:323636/149‑1 (MQ=255)     ggcccgccgATCCCGGCCATGAACGGTGCGGCCCACGACCTGATCGCCGAGCAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCTTGCCGCTGCGGCGCGGCAGgcgctc                                                                                                                                             <  1:274297/146‑3 (MQ=255)            cgATCCCGGCCATGAACGGTGCGGCCCACGACCTGATCGCCGAGCAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACAtg                                                                                                                                      <  3:79670/149‑1 (MQ=255)                 ccGGCCATGAACGGTGCGGCCCACGACCTGATCGCCGAGCAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATgctgct                                                                                                                                 >  6:152627/1‑149 (MQ=255)                 ccGGCCATGAACGGTGCGGCCCAAGACCTGATCGCCGAGCAGTGCGCGAGCATTGCCATCCCAAAGCGTTTTACACTGCCGATCCGCGAGATCTGTGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACAtgctgc                                                                                                                                  <  2:273672/148‑1 (MQ=255)                  cGGCCATGAACGGTGCGGCCCACGACCTGATCGCCGAGCAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGctgctg                                                                                                                                >  5:358052/1‑149 (MQ=255)                   ggCCATGAACGGTGCGGCCCACGACCTGATCGCCGAGCAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGAGCCGCAGCGGCAAGCGCGCCGACATGctgctg                                                                                                                                >  4:332054/1‑148 (MQ=255)                       aTGAACGGTGCGGCCCACGACCTGATCGCCGAGCAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACAtgctgc                                                                                                                                  <  6:412723/142‑1 (MQ=255)                       aTGAACGGTGCGGCCCACGACCTGATCGCCGAGCAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGAGCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACAtgctgc                                                                                                                                  >  5:412723/1‑142 (MQ=255)                         gAACGGTGCGGCCCACGACCTGATCGCCGAGCAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAAcc                                                                                                                         >  8:259407/1‑149 (MQ=255)                         gAACGGTGCGGCCCACGACCTGATCGCCGAGCAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCATGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCAGAAGGACAAcc                                                                                                                         >  8:375100/1‑149 (MQ=255)                               tGCGGCCCACGACCTGATCGCCGAGCAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGtt                                                                                                                   >  7:362609/1‑149 (MQ=255)                                      cACGACCTGATCGCCGAGCAGTGCGCGCGCATTGCCATCCCCACGCGTTTTACCCTGCCTATCCGCGAGATCTGGGACATGCATGAGCGCCTGCCTCGCCGCAGCGGCAAGCGCGCCGAAATGCTTCTGGACAACCCGCGTTTCCgcgc                                                                                                            <  8:369232/149‑1 (MQ=255)                                         gACCTGATCGCCGAGCAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCgg                                                                                                         >  5:143428/1‑149 (MQ=255)                                         gACCTGATCGCCGAGCAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCgg                                                                                                         >  1:361336/1‑149 (MQ=255)                                            cTGATCGCCGAGCAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTa                                                                                                      <  2:267468/149‑1 (MQ=255)                                              gATCGCCGAGCAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTAc                                                                                                     >  2:357032/1‑148 (MQ=255)                                               aTCGCCGAGCAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTAc                                                                                                     <  1:113298/147‑1 (MQ=255)                                               aTCGCCGAGCAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGa                                                                                                   >  5:128797/1‑149 (MQ=255)                                                   ccGAGCAGTGCGCGCGCATTGCCATCCCCAAGCGATTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTc                                                                                               >  1:246503/1‑149 (MQ=255)                                                    cGAGCAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTcc                                                                                              <  8:362609/149‑1 (MQ=255)                                                     gAGCAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCct                                                                                             >  7:145798/1‑149 (MQ=255)                                                     gAGCAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCct                                                                                             <  3:332054/149‑1 (MQ=255)                                                       gCAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCctgc                                                                                           >  3:273955/1‑149 (MQ=255)                                                        cAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCTCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGTACAACCCTCGTTTCCGCGCCGGTTACGACTTCctgct                                                                                          <  7:375100/149‑1 (MQ=255)                                                           tGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCctgctgct                                                                                       <  1:357032/149‑1 (MQ=255)                                                             cgcgcgcATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCtgctgctgc                                                                                     <  7:300294/149‑1 (MQ=255)                                                                gcgcATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGt                                                                                   >  6:363738/1‑148 (MQ=255)                                                                  gcATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGaa                                                                                <  6:38158/149‑1 (MQ=255)                                                                    aTTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAg                                                                              >  3:368274/1‑149 (MQ=255)                                                                     ttGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAgc                                                                             >  4:347618/1‑149 (MQ=255)                                                                       gCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAgcgc                                                                           >  8:200464/1‑149 (MQ=255)                                                                        ccATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGtt                                                                                                       <  2:298663/120‑1 (MQ=255)                                                                        ccATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAAGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGtt                                                                                                       >  1:298663/1‑120 (MQ=255)                                                                           tCCCCAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCggg                                                                       <  6:128797/149‑1 (MQ=255)                                                                             cccAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCgggg                                                                      >  7:204437/1‑148 (MQ=255)                                                                              ccAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACACCCCGAGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGCAAGCGCCgggg                                                                      >  7:339245/1‑147 (MQ=255)                                                                               cAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCcgc                                                                                                              >  8:251288/1‑106 (MQ=255)                                                                                aaGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGgaaga                                                                  <  5:48689/149‑1 (MQ=255)                                                                                     ttttACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAAcc                                                              >  5:163779/1‑148 (MQ=255)                                                                                     ttttACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACcg                                                             <  2:109284/149‑1 (MQ=255)                                                                                         aCCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGTCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCgg                                                                        >  6:155704/1‑134 (MQ=255)                                                                                         aCCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGTCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCgg                                                                        <  5:155704/134‑1 (MQ=255)                                                                                         aCCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCgg                                                                        >  4:223039/1‑134 (MQ=255)                                                                                         aCCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCgg                                                                        <  3:223039/134‑1 (MQ=255)                                                                                         aCCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACcgacg                                                          >  8:182477/1‑148 (MQ=255)                                                                                          cccTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCtgctgctgc                                                                                     <  4:218063/120‑1 (MQ=255)                                                                                          cccTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCtgctgctgc                                                                                     >  3:218063/1‑120 (MQ=255)                                                                                             tGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACCGACGAACTg                                                     >  3:292152/1‑149 (MQ=255)                                                                                               ccGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACCGACGAACTggg                                                   >  6:219711/1‑149 (MQ=255)                                                                                               ccGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACCGACGAACTgg                                                    <  5:152627/148‑1 (MQ=255)                                                                                                   tCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACCGACGAACTGGGCCAg                                               >  5:346233/1‑149 (MQ=255)                                                                                                    ccGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGCAATGCTTCTGGACAACCCTCGTTTCCGAGCCGGTTACGACTTACTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACCGAAGAACTGGGCCact                                              >  3:33157/1‑147 (MQ=255)                                                                                                     cgcgAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACCGACGAACTGGGCCAgtg                                             >  1:345609/1‑149 (MQ=255)                                                                                                      gcgAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACCGCCGAACTGGGCCAgtgg                                            >  8:377966/1‑149 (MQ=255)                                                                                                      gcgAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACCGACGAACTGGGCCAgtgg                                            >  3:102141/1‑149 (MQ=255)                                                                                                       cgAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACCGACGAACTGGGCCAgtggt                                           <  4:102141/149‑1 (MQ=255)                                                                                                         agaTCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACCGACGAACTGGGCCAGtggtgg                                         <  7:259407/149‑1 (MQ=255)                                                                                                          gaTCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACCGACGAACTGGGCCAGTGGTGGa                                        >  6:335147/1‑149 (MQ=255)                                                                                                          gaTCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGG‑TACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACCGACGAACTGGGCCAgtggtg                                          >  1:200223/1‑146 (MQ=255)                                                                                                          gaTCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGG‑TACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACCGACGAACTGGGCCAgtggtg                                          <  2:200223/146‑1 (MQ=255)                                                                                                                ggACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACCGACGAACTGGGCCAGTGGTGGACCGAt                                   <  5:335147/148‑1 (MQ=255)                                                                                                                  aCATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACCGACGAACTGGGCCAgtggtg                                          <  8:238593/139‑1 (MQ=255)                                                                                                                  aCATGAAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACCGACGAACTGGGCCAgtggtg                                          >  7:238593/1‑139 (MQ=255)                                                                                                                   cATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACCGACGAACTGGGCCAGTGGTGGACCGATTAcc                               <  4:273955/149‑1 (MQ=255)                                                                                                                     tGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACCGACGAACTGGGCCAGTGGTGGACCGATTACCAg                             <  4:292152/149‑1 (MQ=255)                                                                                                                      gCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGTAGAAACCGACGAACTGGGCCAGTGGTGGACCGATTACCAgg                            >  5:351593/1‑149 (MQ=255)                                                                                                                        aGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACCGACGAACTGGGCCAGTGGTGGACCGATTACCAGCAc                          >  8:108783/1‑149 (MQ=255)                                                                                                                         ggcgcgcTTGCCGCTGCGGCGCGGCAGGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTTCGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACCGACGAACTGGGCCAGTGGTGGACCGATTACCAGGACg                         >  2:127065/4‑149 (MQ=255)                                                                                                                          gcgcgcTTGCCGCTGCGGCGCGGCAGGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACCGACGAACTGGGCCAGTGGTGGACCGATTa                                 >  2:33462/3‑140 (MQ=255)                                                                                                                          gcgcgcTTGCCGCTGCGGCGCGGCAGGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACCGACGAACTGGGCCAGTGGTGGACCGATTa                                 <  1:33462/138‑1 (MQ=255)                                                                                                                              gcCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACCGACGAACTGGGCCAGTGGTGGACCGATTACCAGGACGCAAAc                    <  2:361336/149‑1 (MQ=255)                                                                                                                                cTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACCGACGAACTGGGCCAGTGGTGGACCGATTACCAGGACGCAAACGa                  >  4:136173/1‑149 (MQ=255)                                                                                                                                 tGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAGCCCGCGTTTCCGCGCCGGTTATGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACCGACGAACTGGGCCAGTGGTGGACCGATTACCAGGACGCAAACGa                  >  3:89347/1‑148 (MQ=255)                                                                                                                                    cgcgccgcAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACCGACGAACTGGGCCAGTGGTGGACCGATTACCAGGACGCAAACGACagc              <  6:346233/149‑1 (MQ=255)                                                                                                                                     gcgccgcAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACCGACGAACTGGGCCAGTGGTGGACCGATTACCAGGACGCAAACGACagc              >  2:314603/1‑148 (MQ=255)                                                                                                                                      cgccgcAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACCGACGAACTGGGCCAGTGGTGGACCGATTACCAGGACGCAAACGACagcga            <  2:345609/149‑1 (MQ=255)                                                                                                                                          gcAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACCGACGAACTGGGCCAGTGGTGGACCGATTACCAGGACGCAAACGACAGCGAgcgc        >  2:184404/1‑149 (MQ=255)                                                                                                                                           cAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACCGACGAACTGGGCCTGTGGTGGACCGATTACCAGGACGCAAACGACAGCGAGcgcc       >  5:352187/1‑149 (MQ=255)                                                                                                                                            aGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACCGACGAACTGGGCCAGTGGTGGACCGATTACCAGGACGCAAACGCCAGCGAGcgccg      >  8:188094/1‑149 (MQ=255)                                                                                                                                            aGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACCGACGAACTGGGCCAGTGGTGGACCGATTACCAGGACGCAAACGACAGCGAGcgccg      >  3:47127/1‑149 (MQ=255)                                                                                                                                                gCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACCGACGAACTGGGCCAGTGGTGGACCGATTACCAGGACGCAAACGACAGCGAGcgccgc     >  2:291968/1‑146 (MQ=255)                                                                                                                                                gCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCGGCTGCGTGAAAGCGCCGGGGAAGAAACCGACGAACTGGGCCAGTGGTGGACCGATTACCAGGACGCAAACGACAGCGAGCGCCGCgag  <  8:145798/149‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                  GGGCGTGCCGCCGATCCCGGCCATGAACGGTGCGGCCCACGACCTGATCGCCGAGCAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACCGACGAACTGGGCCAGTGGTGGACCGATTACCAGGACGCAAACGACAGCGAGCGCCGCGAG  >  NC_002947/5339762‑5340053

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑