Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,343,615 C→G 9.5% I285M (ATC→ATG)  pgi‑II → glucose 6‑phosphate isomerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,343,6150CG 9.5% 128.7 / 5.4 51I285M (ATC→ATG) pgi‑IIglucose 6‑phosphate isomerase
Reads supporting (aligned to +/- strand):  ref base C (30/16);  new base G (3/2);  total (33/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GAAGCCGAGCTGTACCGCCACTTCATTGCCGTATCCAGCAACAAGGCCGCTGCTGTAGCGTTCGGTATCCGCGAAGAAAACATCTTCCCAATGTGGGACTGGGTGGGCGGGCGCTACTCGCTGTGGTCGGCCATCGGCCTGCCGATCGCCCTGGCCATCGGCACTGCCAACTTCAAGGAACTGCTGTCCGGCGCCTACACCATGGACCAGCACTTCCAGACCGCACCGTTCGACAAGAACATGCCGGTGCTACTGGCCCTGCTGGGCGTGTGGTATGGCAACTTCTGG  >  NC_002947/5343469‑5343756
                                                                                                                                                  |                                                                                                                                             
gAAGCCGAGCTGTACCGCCACTTCATTGCCGTATCCAGCAACAAGGCCGCTGCTGTAGCGTTCGGTATCCGCGAAGAAAACATCTTCCCAATGTGGGACTGGGTGGGCGGGCGCTACTCGCTGTGGTCGGCCATCGGCCTGCCGATCGc                                                                                                                                             >  8:43186/1‑149 (MQ=34)
 aaGCCGAGCTGTACCGCCACTTCATTGCCGTATCCAGCAACAAGGCCGCTGCTGTAGCGTTCGGTATCCGCGAAGAAAACATCTTCCCAATGTGGGACTGGGTGGGCGGGCGCTACTCGCTGTGGTCGGCCATCGGCCTGCCGATCGcc                                                                                                                                            >  3:236524/1‑149 (MQ=34)
   gCCGAGCTGTACCGCCACTTCATTGCCGTATCCAGCAACAAGGCCGCTGCTGTAGCGTTCGGTATCCGCGAAGAAAACATCTTCCCAATGTGGGACTGGGTGGGCGGGCGCTACTCGCTGTGGTCGGCCATCGGCCTGCCGATCGCCCt                                                                                                                                          >  5:302753/1‑149 (MQ=34)
           gTACCGCCACTTCATTGCCGTATCCAGCAACAAGGCCGCTGCTGTAGCGTTCGGTATCCGCGAAGAAAACATCTTCCCAATGTGGGACTGGGTGGGCGGGCGCTACTCGCTGTGGTCGGCCATCGGCCTGCCGATCGCCCTGGCCATCg                                                                                                                                  >  3:289704/1‑149 (MQ=34)
              ccGCCACTTCATTGCCGTATCCAGCAACAAGGCCGCTGCTGTAGCGTTCGGTATCCGCGAAGAAAACATCTTCCCAATGTGGGACTGGGTGGGCGGGCGCTACTCGCTGTGGTCGGCCATCGGCCTGCCGATCGCCCTGGCCATCGGCa                                                                                                                               >  1:197757/1‑149 (MQ=34)
              ccGCCACTTCATTGCCGTATCCAGCAACAAGGCCGCTGCTGTAGCGTTCGGTATCCGCGAAGAAAACATCTTCCCAATGTGGGACTGGGTGGGCGGGCGCTACTCGCTGTGGTCGGCCATCGGCCTGCCGATCGCCCTGGCCATCGGCa                                                                                                                               >  1:34427/1‑149 (MQ=34)
                    cTTCATTGCCGTATCCAGCAACAAGGCCGCTGCTGTAGCGTTCGGTATCCGCGAAGAAAACATCTTCCCAATGTGGGACTGGGTGGGCGGGCGCTACTCGCTGTGGTCGGCCATCGGCCTGCCGATCGCCCTGGCCATCGGCACTGcc                                                                                                                          >  8:126443/1‑148 (MQ=34)
                     ttCATTGCCGTATCCAGCAACAAGGCCGCTGCTGTAGCGTTCGGTATCCGCGAAGAAAACATCTTCCCAATGTGGGACTGGGTGGGCGGGCGCTACTCGCTGTGGTCGGCCATCGGCCTGCCGATCGCCCTGGCCATCGGCACTGCCaa                                                                                                                        >  1:346282/1‑149 (MQ=34)
                                     gCAACAAGGCCGCTGCTGTAGCGTTCGGTATCCGCGAAGAAAACATCTTCCCAATGTGGGACTGGGTGGGCGGGCGCTACTCGCTGTGGTCGGCCATCGGCCTGCCGATCGCCCTGGCCATCGGCACTGCCAACTTCAAGGAActgctg                                                                                                        >  7:144049/1‑149 (MQ=34)
                                             gCCGCTGCTGTAGCGTTCGGTATCCGCGAAGAAAACATCTTCCCAATGTGGGACTGGGTGGGCGGGCGCTACTCGCTGTGGTCGGCCATCGGCCTGCCGATCGCCCTGGCCATCGGCACTGCCAACTTCAAGGAACTGCTGTCCGgcgc                                                                                                >  5:255831/1‑149 (MQ=37)
                                              ccGCTGGTGTCGGGTTGGGATCCCGCTAACAAAACACTTCCCCATTGGGGGAGGGGGGGGGGGGGCGCTACTCGCTGTGGTCGGCCATCGGCCTGCCGATCGCCCTGGCCATCGGCACTGCCAACTTCAAGGAACTGCTGTCCGGCGcc                                                                                               <  4:289704/149‑1 (MQ=11)
                                                   ggtttAGCGTGCGTTAACCGCAAATAAAACATCTTCCCATTGGGGGACGGGGGGGGCGGCCGCTACTCGCTGTGGTCGGCCATCGGCCTGCCGATCGCCCTGGCCATCGGCACTGCCAACTTCAAGGAACTGCTGTCCGGCGCCTacac                                                                                          <  7:43186/145‑1 (MQ=11)
                                                     tgTAGCGTTCGGTATCCGCGAAGAAAACATCTTCCCAATGTGGGACTGGGTGGGCGGGCGCTACTCGCTGTGGTCGGCCATCGGCCTGCCGATCGCCCTGGCCATCGGCACTGCCAACTTCAAGGAACTGCTGTCCGGCGCCTACACCa                                                                                        >  6:96001/1‑149 (MQ=37)
                                                           gTTCGTTACCCGCTAACAAAAAATCTCCCAATTGTGGGAGTGGGTGGGCGGGCGCTACTCGCTGTGGTCGGCCATCGGCCTGCCGATCGCCCTGGCCATCGGCACTGCCAACTTCAAGGAACTGCTGTCCGGCGCCTACACCATGGAcc                                                                                  <  2:34427/149‑1 (MQ=11)
                                                                     cgcgAAGAAAACATCTTCCCAATGTGGGACTGGGTGGGCGGGCGCTACTCGCTGTGGTCGGCCATCGGCCTGCCGATCGCCCTGGCCATCGGCACTGCCAACTTCAAGGAACTGCTGTCCGGCGCCTACACCATGGACCAGCACTTc                                                                          >  6:357016/1‑147 (MQ=37)
                                                                           gaaAACATCTTCCCAATGAGGGACTGGGTGGGCGGGCGCTACTCGCTGTGGACGGCCATCGGCCTGCCGATCGCCCAGGCCATCGGCACTGCCAACTTCAAGGAACTCCTGTCCGGCGCCTACACAATGGACCAGCAATTc                                                                          >  5:353344/1‑141 (MQ=11)
                                                                              aaCATCTTCCCAATGTGGGACTGGGTGGGCGGGCGCTACTCGCTGTGGTCGGCCATCGGCCTGCCGATCGCCCTGGCCATCGGCACTGCCAACTTCAAGGAACTGCTGTCCGGCGCCTACACCATGGACCAGCACTTCCAGACCGCAcc                                                               >  8:137636/1‑149 (MQ=37)
                                                                              aaCATCTTCCCAATGTGGGACTGGGTGGGCGGGCGCTACTCGCTGTGGTCGGCCATCGGCCTGCCGATCGCCCTGGCCATCGGCACTGCCAACTTCAAGGAACTGCTGTCCGGCGCCTACACCATGGACCAGCACTTCCAGACCGCAcc                                                               >  8:246432/1‑149 (MQ=37)
                                                                                 aTCTTCCCAATGTGGGACTGGGTGGGCGGGCGCTACTCGCTGTGGTCGGCCATCGGCCTGCCGATCGCCCTGGCCATCGGCACTGCCAACTTCAAGGAACTGCTGTCCGGCGCCTACACCATGGACCAGCACTTCCAGACCGCACCGtt                                                            >  3:313556/1‑149 (MQ=37)
                                                                                          aTGTGGGACTGGGTGGGCGGGCGCTACTCGCTGTGGTCGGCCATCGGCCTGCCGATCGCCCTGGCCATCGGCACTGCCAACTTCAAGGAACTGCTGTCCGGCGCCTACACCATGGACCAGCACTTCCAGACCGCACCGTTCGACaa                                                      >  7:384620/1‑146 (MQ=34)
                                                                                           tgtgGGACTGGGTGGGCGGGCGCTACTCGCTGTGGTCGGCCATCGGCCTGCCGATCGCCCTGGCCATCGGCACTGCCAACTTCAAGGAACTGCTGTCCGGCGCCTACACCATGGACCAGCACTTCCAGACCGCACCGTTCGACAAGaa                                                   >  1:398438/1‑148 (MQ=34)
                                                                                              cggcgggggggggCGGCCGCTACTCGCTGTGGTCGGCCATCGGCCTGCCGATCGCCCTGGCCATCGGCACTGCCAACTTCAAGGAACTGCTGTCCGGCGCCTACACCATGGACCAGCACTTCCAGACCGCACCGTTCGACAAGAACATg                                               <  4:228482/143‑1 (MQ=255)
                                                                                               ggACTGGGTGGGCGGGCGCTACTCGCTGTGGTCGGCCATCGGCCTGCCGATCGCCCTGGCCATCGGCACTGCCAACTTCAAGGAACTGCTGTCCGGCGCCTACACCATGGACCAGCTCTTCCAGACCGCACCGTTCGACAAGAACATGc                                              >  7:321162/1‑149 (MQ=21)
                                                                                               ggACTGGGTGGGCGGGCGCTACTCGCTGTGGTCGGCCATCGGCCTGCCGATCGCCCTGGCCATCGGCACTGCCAACTTCAAGGAACTGCTGTCCGGCGCCTACACCATGGACCAGCACTTCCAGACCGCCCCGTTCGACAAGAACATGc                                              >  8:136068/1‑149 (MQ=21)
                                                                                                 aCTGGGTGGGCGGGCGCTACTCGCTGTGGTCGGCCATCGGCCTGCCGATCGCCCTGGCCATCGGCACTGCCAACTTCAAGGAACTGCTGTCCGGCGCCTACACCATGGACCAGCACTTCCAGACCGCACCGTTCGACAAGAACATGCCg                                            <  5:357016/149‑1 (MQ=34)
                                                                                                  tggggtgggGGGGCGCTACTCGCTGTGGTCGGCCATCGGCCTGCCGATCGCCCTGGCCATCGGCACTGCCAACTTCAAGGAACTGCTGTCCGGCGCCTACACCATGGACCAGCACTTCCAGACCGCACCGTTCGACAAGAACATGCCg                                            <  4:313556/146‑1 (MQ=17)
                                                                                                     ggtgggCGGGCGCTACTCGCTGTGGTCGGCCATCGGCCTGCCGATCGCCCTGGCCATCGGCACTGCCAACTTCAAGGAACTGCTGTCCGGCGCCTACACCATGGACCAGCACTTCCAGACCGCACCGTTCGACAAGAACATGCCGGTGc                                        >  8:241042/1‑149 (MQ=34)
                                                                                                       tgggCGGGCGCTACTCGCTGTGGTCGGCCATCGGCCTGCCGATCGCCCTGGCCATCGGCACTGCCAACTTCAAGGAACTGCTGTCCGGCGCCTACACCATGGACCAGCACTTCCAGACCGCACCGTTCGACAAGAACATGCCGGTGCTa                                      >  2:166321/1‑149 (MQ=34)
                                                                                                        gggcggccGGTACTCGCTGTGGTCGGCGATCGGCCTGCCGATCGCCCTGGCCATCGGGACTGGCATCTTCAAGGAACTGCTGTCCGGCGCCTACACCATGGACCAGCACTTCCAGACGGCACCGTTCGACAAGAACATGCCGGTGCTAc                                     <  8:144049/149‑1 (MQ=11)
                                                                                                             ggcgCTACTCGCTGTGGTCGGCCATCGGCCTGCCGATCGCCCTGGCCATCGGCACTGCCAACTTCAAGGAACTGCTGTCCGGCGCCTACACCATGGACCAGCACTTCCAGACCGCACCGTTCGGCAAGAACATGCCGGTGCTACTGGcc                                <  1:228875/149‑1 (MQ=17)
                                                                                                                  tACTCGCTGTGGTCGGCCATCGGCCTGCCGATCGCCCTGGCCATCGGCACTGCCAACTTCAAGGAACTGCTGTCCGGCGCCTACACCATGGACCAGCACTTCCAGACCGCACCGTTCGACAAGAACATGCCGGTGCTACTGGCCctgct                           >  3:51770/1‑149 (MQ=32)
                                                                                                                    cTCGCTGTGGTCGGCCATCGGCCTGCCGATCGCCCTGGCCATCGGCACTGCCAACTTCAAGGAACTGCTGTCCGGCGCCTACACCATGGACCAGCACTTCCAGACCGCACCGTTCGACAAGAACATGCCGGTGCTACTGGcatgttc                           <  1:166321/147‑7 (MQ=17)
                                                                                                                     tCGCTGTGGTCGGCCATCGGCCTGCCGATCGCCCTGGCCATCGGCACTGCCAACTTCAAGGAACTGCTGTCCGGCGCCTACACCATGGACCAGCACTTCCAGACCGCACCGTTCGACAAGAACATGCCGGTGCTACTGGCCCTGCTggg                        >  2:354958/1‑149 (MQ=32)
                                                                                                                       gCTGTGGTCGGCCATCGGCCTGCCGATCGCCCTGGCCATCGGCACTGCCAACTTCAAGGAACTGCTGTCCGGCGCCTACACCATGGACCAGCACTTCCAGACCGCACCGTTCGACAAGAACATGCCGGTGCTACTGGc                                 <  1:119932/138‑1 (MQ=32)
                                                                                                                       gCTGTGGTCGGCCATCGGCCTGCCGATCGCCCTGGCCATCGGCACTGCCAACTTCAAGGAACTGCTGTCCGGCGCCTACACCATGGACCAGCACTTCCAGACCGCACCGTTCGACAAGAACATGCCGGTGCTACTGGc                                 >  2:119932/1‑138 (MQ=32)
                                                                                                                       gCTGTGGTCGGCCATCGGCCTGCCGATCGCCCTGGCCATCGGCACTGCCAACTTCAAGGAACTGCTGTCCGGCGCCTACACCATGGACCAGCACTTCCAGACCGCACCGTTCGACAAGAACATGCCGGTGCTACTGGCCCTGCTGGGCg                      >  3:109468/1‑149 (MQ=32)
                                                                                                                           tgGTCGGCCATCGGCCTGCCGATCGCCCTGGCCATCGGCACTGCCAACTTCAAGGAACTGCTGTCCGGCGCCTACACCATGGACCAGCACTTCCAGACCGCACCGTTCGACAAGAACATGCCGGTGCTACTGGCCCTGCTGGGCgtgt                   <  5:96001/148‑1 (MQ=32)
                                                                                                                           tgGTCGGCCATCGGCCTGCCGATCGCCCTGGCCATCGGCACTGCCAACTTCAAGGAACTGCTGTCCGGCGCCTACACCATGGACCAGCACTTCCAGACCGCACCGTTCGACAAGAACATGCCGGTGCTACTGGCCCTGCTGGGCgtgt                   <  7:137636/148‑1 (MQ=32)
                                                                                                                             cagggCGATCGGCAGGCCGATGGCCCTGGCCATCGGCACTGCCAACTTCAAGGAACTGCTGTCCGGCGCCTACACCATGGACCAGCACTTCCAGACCGCACCGTTCGACAAGAACATGCCGGTGCTACTGGCCCTGCTGGGCGTGTgg                 >  7:83519/4‑148 (MQ=11)
                                                                                                                                ggccgatggCCTGCCGATCGCCCTGGCCATCGGCACTGCCAACTTCAAGGAACTGCTGTCCGGCGCCTACACCATGGACCAGCACTTCCAGACCGCACCGTTCGACAAGAACATGCCGGTGCTACTGGCCCTGCTGGGCGTGTGGTATg             <  8:349691/142‑1 (MQ=17)
                                                                                                                                ggCCATCGGCCTGCCGATCGCCCTGGCCATCGGCACTGCCAACTTCAAGGAACTGCTGTCCGGCGCCTACACCATGGACCAGCACTTCCAGACCGCACCGTTCGACAAGAACATGCCGGTGCTACTGGCCCTGCTGGGCGtgtg                  >  3:147358/1‑144 (MQ=32)
                                                                                                                                ggCCATCGGCCTGCCGATCGCCCTGGCCATCGGCACTGCCAACTTCAAGGAACTGCTGTCCGGCGCCTACACCATGGACCAGCACTTCCAGACCGCACCGTTCGACAAGAACATGCCGGTGCTACTGGCCCTGCTGGGCGtgtg                  <  4:147358/144‑1 (MQ=32)
                                                                                                                                  ccATCGGCCTGCCGATCGCCCTGGCCATCGGCACTGCCAACTTCAAGGAACTGCTGTCCGGCGCCTACACCATGGACCAGCACTTCCAGACCGCACCGTTCGACAAGAACATGCCGGTGCTACTGGCCCTGCTGGGCGTGTGGTATgg            >  2:172347/1‑148 (MQ=32)
                                                                                                                                  ccATCGGCCTGCCGATCGCCCTGGCCATCGGCACTGCCAACTTCAAGGAACTGCTGTCCGGCGCCTACACCATGGACCAGCACTTCCAGACCGCACCGTTCGACAAGAACATGCCGGTGCTACTGGCCCTGCTGGGCGTGTGGTATGGc           <  2:346282/149‑1 (MQ=32)
                                                                                                                                   cATCGGCCTGCCGATCGCCCTGGCCATCGGCACTGCCAACTTCAAGGAACTGCTGTCCGGCGCCTACACCATGGACCAGCACTTCCAGACCGCACCGTTCGACAAGAACATGCCGGTGCTACTGGCCCTGCTGGGCGTGTGGTATGGc           >  1:353996/1‑148 (MQ=32)
                                                                                                                                           tGCCGATGGCCAGGGCGATCGGCACTGCCAACTTCAAGGAACTGCTGTCCGGCGCCTACACCATGGACCAGCACTTCCAGACCGCACCGTTCGACAAGAACATGCCGGTGCTACTGGCCCTGCTGGGCGTGTGGTATg             <  3:340343/138‑1 (MQ=255)
                                                                                                                                           tGCCGATGGCCAGGGCGATCGGCACTGCCAACTTCAAGGAACTGCTGTCCGGCGCCTACACCATGGACCAGCACTTCCAGACCGCACCGTTCGACAAGAACATGCCGGTGCTACTGGCCCTGCTGGGCGTGTGGTATg             <  3:346180/138‑1 (MQ=255)
                                                                                                                                           tGCCGATGGCCAGGGCGATCGGCACTGCCAACTTCAAGGAACTGCTGTCCGGCGCCTACACCATGGACCAGCACTTCCAGACCGCACCGTTCGACAAGAACATGCCGGTGCTACTGGCCCTGCTGGGCGTGTGGTATg             >  4:346180/1‑138 (MQ=255)
                                                                                                                                           tGCCGATGGCCAGGGCGATCGGCACTGCCAACTTCAAGGAACTGCTGTCCGGCGCCTACACCATGGACCAGCACTTCCAGACCGCACCGTTCGACAAGAACATGCCGGTGCTACTGGCCCTGCTGGGCGTGTGGTATg             >  4:340343/1‑138 (MQ=255)
                                                                                                                                           tGCCGATCGCCCTGGCCATCGGCACTGCCAACTTCAAGGAACTGCTGTCCGGCGCCTACACCATGGACCAGCACTTCCAGACCGCACCGTTCGACAAGAACATGCCGGTGCTACTGGCCCTGCTGGGCGTGTGGTATGGCAACTTCTgg  >  4:143749/1‑149 (MQ=32)
                                                                                                                                             ccGATCGCCCTGGCCATCGGCACTGCCAACTTCAAGGAACTGCTGTCCGGCGCCTACACCATGGACCAGCACTTCCAGACCGCACCGTTCGACAAGAACATGCCGGTGCTACTGGCCCTGCTGGGCGTGTGGTATGGCAACTTCTgg  <  4:109468/147‑1 (MQ=32)
                                                                                                                                                  |                                                                                                                                             
GAAGCCGAGCTGTACCGCCACTTCATTGCCGTATCCAGCAACAAGGCCGCTGCTGTAGCGTTCGGTATCCGCGAAGAAAACATCTTCCCAATGTGGGACTGGGTGGGCGGGCGCTACTCGCTGTGGTCGGCCATCGGCCTGCCGATCGCCCTGGCCATCGGCACTGCCAACTTCAAGGAACTGCTGTCCGGCGCCTACACCATGGACCAGCACTTCCAGACCGCACCGTTCGACAAGAACATGCCGGTGCTACTGGCCCTGCTGGGCGTGTGGTATGGCAACTTCTGG  >  NC_002947/5343469‑5343756

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑