| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,343,619 | C→A | 8.2% | L287M (CTG→ATG) | pgi‑II → | glucose 6‑phosphate isomerase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,343,619 | 0 | C | A | 8.2% | 117.5 / 4.4 | 49 | L287M (CTG→ATG) | pgi‑II | glucose 6‑phosphate isomerase |
| Reads supporting (aligned to +/- strand): ref base C (29/16); new base A (2/2); total (31/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.18e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.45e-01 | |||||||||||
GCCGAGCTGTACCGCCACTTCATTGCCGTATCCAGCAACAAGGCCGCTGCTGTAGCGTTCGGTATCCGCGAAGAAAACATCTTCCCAATGTGGGACTGGGTGGGCGGGCGCTACTCGCTGTGGTCGGCCATCGGCCTGCCGATCGCCCTGGCCATCGGCACTGCCAACTTCAAGGAACTGCTGTCCGGCGCCTACACCATGGACCAGCACTTCCAGACCGCACCGTTCGACAAGAACATGCCGGTGCTACTGGCCCTGCTGGGCGTGTGGTATGGCAACTTCTGGGACGCAAACA > NC_002947/5343472‑5343766 | gCCGAGCTGTACCGCCACTTCATTGCCGTATCCAGCAACAAGGCCGCTGCTGTAGCGTTCGGTATCCGCGAAGAAAACATCTTCCCAATGTGGGACTGGGTGGGCGGGCGCTACTCGCTGTGGTCGGCCATCGGCCTGCCGATCGCCCt > 5:302753/1‑149 (MQ=34) gTACCGCCACTTCATTGCCGTATCCAGCAACAAGGCCGCTGCTGTAGCGTTCGGTATCCGCGAAGAAAACATCTTCCCAATGTGGGACTGGGTGGGCGGGCGCTACTCGCTGTGGTCGGCCATCGGCCTGCCGATCGCCCTGGCCATCg > 3:289704/1‑149 (MQ=34) ccGCCACTTCATTGCCGTATCCAGCAACAAGGCCGCTGCTGTAGCGTTCGGTATCCGCGAAGAAAACATCTTCCCAATGTGGGACTGGGTGGGCGGGCGCTACTCGCTGTGGTCGGCCATCGGCCTGCCGATCGCCCTGGCCATCGGCa > 1:197757/1‑149 (MQ=34) ccGCCACTTCATTGCCGTATCCAGCAACAAGGCCGCTGCTGTAGCGTTCGGTATCCGCGAAGAAAACATCTTCCCAATGTGGGACTGGGTGGGCGGGCGCTACTCGCTGTGGTCGGCCATCGGCCTGCCGATCGCCCTGGCCATCGGCa > 1:34427/1‑149 (MQ=34) cTTCATTGCCGTATCCAGCAACAAGGCCGCTGCTGTAGCGTTCGGTATCCGCGAAGAAAACATCTTCCCAATGTGGGACTGGGTGGGCGGGCGCTACTCGCTGTGGTCGGCCATCGGCCTGCCGATCGCCCTGGCCATCGGCACTGcc > 8:126443/1‑148 (MQ=34) ttCATTGCCGTATCCAGCAACAAGGCCGCTGCTGTAGCGTTCGGTATCCGCGAAGAAAACATCTTCCCAATGTGGGACTGGGTGGGCGGGCGCTACTCGCTGTGGTCGGCCATCGGCCTGCCGATCGCCCTGGCCATCGGCACTGCCaa > 1:346282/1‑149 (MQ=34) gCAACAAGGCCGCTGCTGTAGCGTTCGGTATCCGCGAAGAAAACATCTTCCCAATGTGGGACTGGGTGGGCGGGCGCTACTCGCTGTGGTCGGCCATCGGCCTGCCGATCGCCCTGGCCATCGGCACTGCCAACTTCAAGGAActgctg > 7:144049/1‑149 (MQ=34) gCCGCTGCTGTAGCGTTCGGTATCCGCGAAGAAAACATCTTCCCAATGTGGGACTGGGTGGGCGGGCGCTACTCGCTGTGGTCGGCCATCGGCCTGCCGATCGCCCTGGCCATCGGCACTGCCAACTTCAAGGAACTGCTGTCCGgcgc > 5:255831/1‑149 (MQ=37) ccGCTGGTGTCGGGTTGGGATCCCGCTAACAAAACACTTCCCCATTGGGGGAGGGGGGGGGGGGGCGCTACTCGCTGTGGTCGGCCATCGGCCTGCCGATCGCCCTGGCCATCGGCACTGCCAACTTCAAGGAACTGCTGTCCGGCGcc < 4:289704/149‑1 (MQ=11) ggtttAGCGTGCGTTAACCGCAAATAAAACATCTTCCCATTGGGGGACGGGGGGGGCGGCCGCTACTCGCTGTGGTCGGCCATCGGCCTGCCGATCGCCCTGGCCATCGGCACTGCCAACTTCAAGGAACTGCTGTCCGGCGCCTacac < 7:43186/145‑1 (MQ=11) tgTAGCGTTCGGTATCCGCGAAGAAAACATCTTCCCAATGTGGGACTGGGTGGGCGGGCGCTACTCGCTGTGGTCGGCCATCGGCCTGCCGATCGCCCTGGCCATCGGCACTGCCAACTTCAAGGAACTGCTGTCCGGCGCCTACACCa > 6:96001/1‑149 (MQ=37) gTTCGTTACCCGCTAACAAAAAATCTCCCAATTGTGGGAGTGGGTGGGCGGGCGCTACTCGCTGTGGTCGGCCATCGGCCTGCCGATCGCCCTGGCCATCGGCACTGCCAACTTCAAGGAACTGCTGTCCGGCGCCTACACCATGGAcc < 2:34427/149‑1 (MQ=11) cgcgAAGAAAACATCTTCCCAATGTGGGACTGGGTGGGCGGGCGCTACTCGCTGTGGTCGGCCATCGGCCTGCCGATCGCCCTGGCCATCGGCACTGCCAACTTCAAGGAACTGCTGTCCGGCGCCTACACCATGGACCAGCACTTc > 6:357016/1‑147 (MQ=37) gaaAACATCTTCCCAATGAGGGACTGGGTGGGCGGGCGCTACTCGCTGTGGACGGCCATCGGCCTGCCGATCGCCCAGGCCATCGGCACTGCCAACTTCAAGGAACTCCTGTCCGGCGCCTACACAATGGACCAGCAATTc > 5:353344/1‑141 (MQ=11) aaCATCTTCCCAATGTGGGACTGGGTGGGCGGGCGCTACTCGCTGTGGTCGGCCATCGGCCTGCCGATCGCCCTGGCCATCGGCACTGCCAACTTCAAGGAACTGCTGTCCGGCGCCTACACCATGGACCAGCACTTCCAGACCGCAcc > 8:246432/1‑149 (MQ=37) aaCATCTTCCCAATGTGGGACTGGGTGGGCGGGCGCTACTCGCTGTGGTCGGCCATCGGCCTGCCGATCGCCCTGGCCATCGGCACTGCCAACTTCAAGGAACTGCTGTCCGGCGCCTACACCATGGACCAGCACTTCCAGACCGCAcc > 8:137636/1‑149 (MQ=37) aTCTTCCCAATGTGGGACTGGGTGGGCGGGCGCTACTCGCTGTGGTCGGCCATCGGCCTGCCGATCGCCCTGGCCATCGGCACTGCCAACTTCAAGGAACTGCTGTCCGGCGCCTACACCATGGACCAGCACTTCCAGACCGCACCGtt > 3:313556/1‑149 (MQ=37) aTGTGGGACTGGGTGGGCGGGCGCTACTCGCTGTGGTCGGCCATCGGCCTGCCGATCGCCCTGGCCATCGGCACTGCCAACTTCAAGGAACTGCTGTCCGGCGCCTACACCATGGACCAGCACTTCCAGACCGCACCGTTCGACaa > 7:384620/1‑146 (MQ=34) tgtgGGACTGGGTGGGCGGGCGCTACTCGCTGTGGTCGGCCATCGGCCTGCCGATCGCCCTGGCCATCGGCACTGCCAACTTCAAGGAACTGCTGTCCGGCGCCTACACCATGGACCAGCACTTCCAGACCGCACCGTTCGACAAGaa > 1:398438/1‑148 (MQ=34) cggcgggggggggCGGCCGCTACTCGCTGTGGTCGGCCATCGGCCTGCCGATCGCCCTGGCCATCGGCACTGCCAACTTCAAGGAACTGCTGTCCGGCGCCTACACCATGGACCAGCACTTCCAGACCGCACCGTTCGACAAGAACATg < 4:228482/143‑1 (MQ=255) ggACTGGGTGGGCGGGCGCTACTCGCTGTGGTCGGCCATCGGCCTGCCGATCGCCCTGGCCATCGGCACTGCCAACTTCAAGGAACTGCTGTCCGGCGCCTACACCATGGACCAGCTCTTCCAGACCGCACCGTTCGACAAGAACATGc > 7:321162/1‑149 (MQ=21) ggACTGGGTGGGCGGGCGCTACTCGCTGTGGTCGGCCATCGGCCTGCCGATCGCCCTGGCCATCGGCACTGCCAACTTCAAGGAACTGCTGTCCGGCGCCTACACCATGGACCAGCACTTCCAGACCGCCCCGTTCGACAAGAACATGc > 8:136068/1‑149 (MQ=21) aCTGGGTGGGCGGGCGCTACTCGCTGTGGTCGGCCATCGGCCTGCCGATCGCCCTGGCCATCGGCACTGCCAACTTCAAGGAACTGCTGTCCGGCGCCTACACCATGGACCAGCACTTCCAGACCGCACCGTTCGACAAGAACATGCCg < 5:357016/149‑1 (MQ=34) tggggtgggGGGGCGCTACTCGCTGTGGTCGGCCATCGGCCTGCCGATCGCCCTGGCCATCGGCACTGCCAACTTCAAGGAACTGCTGTCCGGCGCCTACACCATGGACCAGCACTTCCAGACCGCACCGTTCGACAAGAACATGCCg < 4:313556/146‑1 (MQ=17) ggtgggCGGGCGCTACTCGCTGTGGTCGGCCATCGGCCTGCCGATCGCCCTGGCCATCGGCACTGCCAACTTCAAGGAACTGCTGTCCGGCGCCTACACCATGGACCAGCACTTCCAGACCGCACCGTTCGACAAGAACATGCCGGTGc > 8:241042/1‑149 (MQ=34) tgggCGGGCGCTACTCGCTGTGGTCGGCCATCGGCCTGCCGATCGCCCTGGCCATCGGCACTGCCAACTTCAAGGAACTGCTGTCCGGCGCCTACACCATGGACCAGCACTTCCAGACCGCACCGTTCGACAAGAACATGCCGGTGCTa > 2:166321/1‑149 (MQ=34) gggcggccGGTACTCGCTGTGGTCGGCGATCGGCCTGCCGATCGCCCTGGCCATCGGGACTGGCATCTTCAAGGAACTGCTGTCCGGCGCCTACACCATGGACCAGCACTTCCAGACGGCACCGTTCGACAAGAACATGCCGGTGCTAc < 8:144049/149‑1 (MQ=11) ggcgCTACTCGCTGTGGTCGGCCATCGGCCTGCCGATCGCCCTGGCCATCGGCACTGCCAACTTCAAGGAACTGCTGTCCGGCGCCTACACCATGGACCAGCACTTCCAGACCGCACCGTTCGGCAAGAACATGCCGGTGCTACTGGcc < 1:228875/149‑1 (MQ=17) tACTCGCTGTGGTCGGCCATCGGCCTGCCGATCGCCCTGGCCATCGGCACTGCCAACTTCAAGGAACTGCTGTCCGGCGCCTACACCATGGACCAGCACTTCCAGACCGCACCGTTCGACAAGAACATGCCGGTGCTACTGGCCctgct > 3:51770/1‑149 (MQ=32) cTCGCTGTGGTCGGCCATCGGCCTGCCGATCGCCCTGGCCATCGGCACTGCCAACTTCAAGGAACTGCTGTCCGGCGCCTACACCATGGACCAGCACTTCCAGACCGCACCGTTCGACAAGAACATGCCGGTGCTACTGGcatgttc < 1:166321/147‑7 (MQ=17) tCGCTGTGGTCGGCCATCGGCCTGCCGATCGCCCTGGCCATCGGCACTGCCAACTTCAAGGAACTGCTGTCCGGCGCCTACACCATGGACCAGCACTTCCAGACCGCACCGTTCGACAAGAACATGCCGGTGCTACTGGCCCTGCTggg > 2:354958/1‑149 (MQ=32) gCTGTGGTCGGCCATCGGCCTGCCGATCGCCCTGGCCATCGGCACTGCCAACTTCAAGGAACTGCTGTCCGGCGCCTACACCATGGACCAGCACTTCCAGACCGCACCGTTCGACAAGAACATGCCGGTGCTACTGGc < 1:119932/138‑1 (MQ=32) gCTGTGGTCGGCCATCGGCCTGCCGATCGCCCTGGCCATCGGCACTGCCAACTTCAAGGAACTGCTGTCCGGCGCCTACACCATGGACCAGCACTTCCAGACCGCACCGTTCGACAAGAACATGCCGGTGCTACTGGc > 2:119932/1‑138 (MQ=32) gCTGTGGTCGGCCATCGGCCTGCCGATCGCCCTGGCCATCGGCACTGCCAACTTCAAGGAACTGCTGTCCGGCGCCTACACCATGGACCAGCACTTCCAGACCGCACCGTTCGACAAGAACATGCCGGTGCTACTGGCCCTGCTGGGCg > 3:109468/1‑149 (MQ=32) tgGTCGGCCATCGGCCTGCCGATCGCCCTGGCCATCGGCACTGCCAACTTCAAGGAACTGCTGTCCGGCGCCTACACCATGGACCAGCACTTCCAGACCGCACCGTTCGACAAGAACATGCCGGTGCTACTGGCCCTGCTGGGCgtgt < 5:96001/148‑1 (MQ=32) tgGTCGGCCATCGGCCTGCCGATCGCCCTGGCCATCGGCACTGCCAACTTCAAGGAACTGCTGTCCGGCGCCTACACCATGGACCAGCACTTCCAGACCGCACCGTTCGACAAGAACATGCCGGTGCTACTGGCCCTGCTGGGCgtgt < 7:137636/148‑1 (MQ=32) cagggCGATCGGCAGGCCGATGGCCCTGGCCATCGGCACTGCCAACTTCAAGGAACTGCTGTCCGGCGCCTACACCATGGACCAGCACTTCCAGACCGCACCGTTCGACAAGAACATGCCGGTGCTACTGGCCCTGCTGGGCGTGTgg > 7:83519/4‑148 (MQ=11) ggccgatggCCTGCCGATCGCCCTGGCCATCGGCACTGCCAACTTCAAGGAACTGCTGTCCGGCGCCTACACCATGGACCAGCACTTCCAGACCGCACCGTTCGACAAGAACATGCCGGTGCTACTGGCCCTGCTGGGCGTGTGGTATg < 8:349691/142‑1 (MQ=17) ggCCATCGGCCTGCCGATCGCCCTGGCCATCGGCACTGCCAACTTCAAGGAACTGCTGTCCGGCGCCTACACCATGGACCAGCACTTCCAGACCGCACCGTTCGACAAGAACATGCCGGTGCTACTGGCCCTGCTGGGCGtgtg > 3:147358/1‑144 (MQ=32) ggCCATCGGCCTGCCGATCGCCCTGGCCATCGGCACTGCCAACTTCAAGGAACTGCTGTCCGGCGCCTACACCATGGACCAGCACTTCCAGACCGCACCGTTCGACAAGAACATGCCGGTGCTACTGGCCCTGCTGGGCGtgtg < 4:147358/144‑1 (MQ=32) ccATCGGCCTGCCGATCGCCCTGGCCATCGGCACTGCCAACTTCAAGGAACTGCTGTCCGGCGCCTACACCATGGACCAGCACTTCCAGACCGCACCGTTCGACAAGAACATGCCGGTGCTACTGGCCCTGCTGGGCGTGTGGTATgg > 2:172347/1‑148 (MQ=32) ccATCGGCCTGCCGATCGCCCTGGCCATCGGCACTGCCAACTTCAAGGAACTGCTGTCCGGCGCCTACACCATGGACCAGCACTTCCAGACCGCACCGTTCGACAAGAACATGCCGGTGCTACTGGCCCTGCTGGGCGTGTGGTATGGc < 2:346282/149‑1 (MQ=32) cATCGGCCTGCCGATCGCCCTGGCCATCGGCACTGCCAACTTCAAGGAACTGCTGTCCGGCGCCTACACCATGGACCAGCACTTCCAGACCGCACCGTTCGACAAGAACATGCCGGTGCTACTGGCCCTGCTGGGCGTGTGGTATGGc > 1:353996/1‑148 (MQ=32) tGCCGATGGCCAGGGCGATCGGCACTGCCAACTTCAAGGAACTGCTGTCCGGCGCCTACACCATGGACCAGCACTTCCAGACCGCACCGTTCGACAAGAACATGCCGGTGCTACTGGCCCTGCTGGGCGTGTGGTATg < 3:346180/138‑1 (MQ=255) tGCCGATGGCCAGGGCGATCGGCACTGCCAACTTCAAGGAACTGCTGTCCGGCGCCTACACCATGGACCAGCACTTCCAGACCGCACCGTTCGACAAGAACATGCCGGTGCTACTGGCCCTGCTGGGCGTGTGGTATg < 3:340343/138‑1 (MQ=255) tGCCGATGGCCAGGGCGATCGGCACTGCCAACTTCAAGGAACTGCTGTCCGGCGCCTACACCATGGACCAGCACTTCCAGACCGCACCGTTCGACAAGAACATGCCGGTGCTACTGGCCCTGCTGGGCGTGTGGTATg > 4:346180/1‑138 (MQ=255) tGCCGATGGCCAGGGCGATCGGCACTGCCAACTTCAAGGAACTGCTGTCCGGCGCCTACACCATGGACCAGCACTTCCAGACCGCACCGTTCGACAAGAACATGCCGGTGCTACTGGCCCTGCTGGGCGTGTGGTATg > 4:340343/1‑138 (MQ=255) tGCCGATCGCCCTGGCCATCGGCACTGCCAACTTCAAGGAACTGCTGTCCGGCGCCTACACCATGGACCAGCACTTCCAGACCGCACCGTTCGACAAGAACATGCCGGTGCTACTGGCCCTGCTGGGCGTGTGGTATGGCAACTTCTgg > 4:143749/1‑149 (MQ=32) ccGATCGCCCTGGCCATCGGCACTGCCAACTTCAAGGAACTGCTGTCCGGCGCCTACACCATGGACCAGCACTTCCAGACCGCACCGTTCGACAAGAACATGCCGGTGCTACTGGCCCTGCTGGGCGTGTGGTATGGCAACTTCTgg < 4:109468/147‑1 (MQ=32) cccTGGCCATCGGCACTGCCAACTTCAAGGAACTGCTGTCCGGCGCCTACACCATGGACCAGCACTTCCAGACCGCACCGTTCGACAACAACATGCCGGTGCTACTGGCCCTGCTGGGCGTGTGGTATGGCAACTTCTGGGACGCaaa > 8:105221/1‑148 (MQ=17) ccTGGCCATCGGCACTGCCAACTTCAAGGAACTGCTGTCCGGCGCCTACACCATGGACCAGCACTTCCAGACCGCACCGTTCGACAAGAACATGCCGGTGCTACTGGCCCTGCTGGGCGTGTGGTATGGCAACTTCTGGGACGCaaa > 6:119321/1‑147 (MQ=32) ccTGGCCATCGGCACTGCCAACTTCAAGGAACTGCTGTCCGGCGCCTACACCATGGACCAGCACTTCCAGACCGCACCGTTCGACAAGAACATGCCGGTGCTACTGGCCCTGCTGGGCGTGTGGTATGGCAACTTCTGGGACGCaaa > 5:354736/1‑147 (MQ=32) ccTGGCCATCGGCACTGCCAACTTCAAGGAACTGCTGTCCGGCGCCTACACCATGGACCAGCACTTCCAGACCGCACCGTTCGACAAGAACATGCCGGTGCTACTGGCCCTGCTGGGCGTGTGGTATGGCAACTTCTGGGACGCAAACa > 7:95041/1‑149 (MQ=32) | GCCGAGCTGTACCGCCACTTCATTGCCGTATCCAGCAACAAGGCCGCTGCTGTAGCGTTCGGTATCCGCGAAGAAAACATCTTCCCAATGTGGGACTGGGTGGGCGGGCGCTACTCGCTGTGGTCGGCCATCGGCCTGCCGATCGCCCTGGCCATCGGCACTGCCAACTTCAAGGAACTGCTGTCCGGCGCCTACACCATGGACCAGCACTTCCAGACCGCACCGTTCGACAAGAACATGCCGGTGCTACTGGCCCTGCTGGGCGTGTGGTATGGCAACTTCTGGGACGCAAACA > NC_002947/5343472‑5343766 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |