| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,362,215 | C→G | 5.3% | V49L (GTG→CTG) | tpiA ← | triose phosphate isomerase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,362,215 | 0 | C | G | 5.3% | 240.3 / 3.4 | 75 | V49L (GTG→CTG) | tpiA | triose phosphate isomerase |
| Reads supporting (aligned to +/- strand): ref base C (34/37); new base G (2/2); total (36/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.28e-01 | |||||||||||
CGCTCCGAATGGCCAATCAACACCAACTTGCAACCTGCTTCAACCAGCTGCTCGGGAGCAACTTCCCCGGTCAGCGCACCCTGTTCGGGTTGTACAGCAGAATTCTGTGCGCCGACAGTAATTTCTTTACCTGCCAGGCCATCAATCACCTGATTGATGAACAAGGCCGGTGGAAATACG‑GCGACTTCCACTCCGCTCGGCAAGGCGAGATTACTCAAGCCTTTGGTCAGCTCAGCGACGCTAGCGCGGGTACCGTGCATTTTCCAGTTACCAGCTACCATAGGGCGACGCATGCT > NC_002947/5362067‑5362362 | cGCTCCGAATGGCCAATCAACACCAACTTGCAACCTGCTTCAACCAGCTGCTCGGGAGCAACTTCCCCGGTCAGCGCACCCTGTTCGGGTTGTACAGCAGAATTCTGTGCGCCGACAGTAATTTCTTTACCTGCCAGGCCATCAATCAc < 1:341310/149‑1 (MQ=255) cGAATGGCCAATCAACACCAACTTGCATCCTTCTTCAACCAGCTGCTCGGGAGCAACTTCCCCGGTCAGCGCACCCTGTTCGGGTTGTACAGCAGAATTCTGTGCGCCGACAGTAATTTCTTTACCTGCCAGGCCATCAATCAGGtgat < 6:308506/149‑1 (MQ=255) gAATGGCCAATCAACACCAACTTGCAACCTGCTTCAACCAGCTGCTCGGGAGCAACTTCCCCGGTCAGCGCACCCTGTTCGGGTTGTACAGCAGAATTCTGTGCGCCGACAGTAATTTCTTTACCTGCCAGGCCATCAATCACCtgatt < 3:296162/149‑1 (MQ=255) tGGCCAATCAACACCAACTTGCAACCTGCTTCAACCAGCTGCTCGGGAGCAACTTCCCCGGTCAGCGCACCCTGTTCGGGTTGTACAGCAGAATTCTGTGCGCCGACAGTAATTTCTTTACCTGCCAGGCCATCAATCACCtgattgat < 4:112043/149‑1 (MQ=255) gCCAATCAACACCAACTTGCAACCTGCTTCAACCAGCTGCTCGGGAGCAACTTCCCCGGTCAGCGCACCCTGTTCGGGTTGTACAGCAGAATTCTGTGCGCCGACAGTAATTTCTTTACCTGCCAGGCCATCAATCAGGTGATtgatg < 1:184882/148‑1 (MQ=255) ccAATCAACACCAACTTGCAACCTGCTTCAACCAGCTGCTCGGGAGCAACTTCCCCGGTCAGCGCACCCTGTTCGGGTTGTACAGCAGAATTCTGTGCGCCGACAGTAATTTCTTTACCTGCCAGGCCATCAATCACCTGATTGATGaa < 2:37803/149‑1 (MQ=255) cAATCAACACCAACTTGCAACCTGCTTCAACCAGCTGCTCGGGAGCAACTTCCCCGGTCAGCGCACCCTCTTCGGGTTGTACAGCAGAATTCTGTGCGCCGACAGTAATTTCTTTACCTGCCAGGCCATCAATCACCTGATTGATGaa > 3:295720/1‑148 (MQ=255) aTCAACACCAACTTGCAACCTGCTTCAACCAGCTGCTCGGGAGCAACTTCCCCGGTCAGCGCACCCTGTTCGGGTTGTACAGCAGAATTCTGTGCGCCGACAGTAATTTCTTTACCTGCCAGGCCATCAATCACCTGATTGATGAACaa < 4:295720/149‑1 (MQ=255) acacCAACTTGCAACCTGCTTCAACCAGCTGCTCGGGAGCAACTTCCCCGGTCAGCGCACCCTGTTCGGGTTGTACAGCAGAATTCTGTGCGCCGACAGTAATTTCTTTACCTGCCAGGCCATCAATCACCTGATTGATGAACAAGGcc > 8:110718/1‑149 (MQ=255) acacCAACTTGCAACCTGCTTCAACCAGCTGCTCGGGAGCAACTTCCCCGGTCAGCGCACCCTGTTCGGGTTGTACAGCAGAATTCTGTGCGCCGACAGTAATTTCTTTACCTGCCAGGCCATCAATCACCTGATTGATGAACAAGGcc > 7:22792/1‑149 (MQ=255) cacCAACTTGCAACCTGCTTCAACCAGCTGCTCGGGAGCAACTTCCCCGGTCAGCGCACCCTGTTCGGGTTGTACAGCAGAATTCTGTGCGCCGACAGTAATTTCTTTACCTGCCAGGCCATCAATCACCTGATTGATGAACAAGGCCg > 4:64524/1‑149 (MQ=255) aCTTGCAACCTGCTTCAACCAGCTGCTCGGGAGCAACTTCCCCGGTCAGCGCACCCTGTTCGGGTTGTACAGCAGAATTCTGTGCGCCGACAGTAATTTCTTTACCTGCCAGGCCATCAATCACCTGATTGATGAACAAGGCCGGTgg > 4:188554/1‑148 (MQ=255) aCTTGCAACCTGCTTCAACCAGCTGCTCGGGAGCAACTTCCCCGGTCAGCGCACCCTGTTCGGGTTGTACAGCAGAATTCTGTGCGCCGACAGTAATTTCTTTACCTGCCAGGCCATCAATCACCTGATTGATGAACAAGGCCGGTgg > 8:120648/1‑148 (MQ=255) aaCCTGCTTCAACCAGCTGCTCGGGAGCAACTTCCCCGGTCAGCGCACCCTGTTCGGGTTGTACAGCAGAATTCTGTGCGCCGACAGTAATTTCTTTACCTGCCAGGCCATCAATCACCTGATTGATGAACAAGGCCGGTGGAAATACg > 2:332880/1‑149 (MQ=255) cTGCTTCAACCAGCTGCTCGGGAGCAACTTCCCCGGTCAGCGCACCCTGTTCGGGTTGTACAGCAGAATTCTGTGCGCCGACAGTAATTTCTTTACCTGCCAGGCCATCAATCACCTGATTGATGAACAAGGCCGGTGGAAATACG‑Gc > 1:179298/1‑148 (MQ=255) ttCAACCAGCTGCTCGGGAGCAACTTCCCCGGTCAGCGCACCCTGTTCGGGTTGTACAGCAGAATTCTGTGCGCCGACAGTAATTTCTTTACCTGCCAGGCCATCAATCACCTGATTGATGAACAAGGCCGGTGGAAATACG‑GCGACtt > 8:55223/1‑149 (MQ=255) ttCAACCAGCTGCTCGGGAGCAACTTCCCCGGTCAGCGCACCCTGTTCGGGTTGTACAGCAGAATTCTGTGCGCCGACAGTAATTTCTTTACCTGCCAGGCCATCAATCACCTGATTGATGAACAAGGCCGGTGGAAATACG‑GCGACtt > 5:333418/1‑149 (MQ=255) ccAGCTGCTCGGGAGCAACTTCCCCGGTCAGCGCACCCTGTTCGGGTTGTACAGCAGAATTCTGTGCGCCGACAGTAATTTCTTTACCTGCCAGGCCATCAATCACCTGATTGATGAACAAGGCCGGTGG‑AATACG‑GCGACTTCCACTc > 4:376847/1‑149 (MQ=255) cAGCTGCTCGGGAGCAACTTCCCCGGTCAGCGCACCCTGTTCGGGTTGTACAGCAGAATTCTGTGCGCCGACAGTAATTTCTTTACCTGCCAGGCCATCAATCACCTGATTGATGAACAAGGCCGGTGGAAATACG‑GCGACTTCCACTc < 3:188554/149‑1 (MQ=255) gctgctCGGGAGCAACTTCCCCGGTCAGCGCACCCTGTTCGGGTTGTACAGCAGAATTCTGTGCGCCGACAGTAATTTCTTTACCTGCCAGGCCATCAATCACCTGATTGATGAACAAGGCCGGTGGAAATACG‑GCGACTTCCACTCCg > 3:227688/1‑149 (MQ=255) tgctCGGGAGCAACTTCCCCGGTCAGCGCACCCTGTTCGGGTTGTACAGCAGAATTCTGTGCGCCGACAGTAATTTCTTTACCTGCCAGGCCATCAATCACCTGATTGATGAACAAGGCCGGTGGAAATACG‑GCGACTTCCACTCCGCt > 4:191359/1‑149 (MQ=255) agctCGGGAGCAACTTCCCCGGTCAGCGCACCCTGTTCGGGTTGTACAGCAGAATTCTGTGCGCCGACAGTAATTTCTTTACCTGCCAGGCCATCAATCACCTGATTGATGAACAAGGCCGGTGGAAATACG‑GCGACTTCCACTCCGCt < 2:94768/148‑1 (MQ=255) gAGCAACTTCCCCGGTCAGCGCACCCTGTTCGGGTTGTACAGCAGAATTCTGTGCGCCGACAGTAATTTCTTTACCTGCCAGGCCATCAATCACCTGATTGATGAACAAGGCCGGTGGAAATACG‑GCGACTTCCACTCCGCTCGGCAAg > 4:401185/1‑149 (MQ=255) aGCAACTTCCCCGGTCAGCGCACCCTGTTCGGGTTGTACAGCAGAATTCTGTGCGCCGACAGTAATTTCTTTACCTGCCAGGCCATCAATCACCTGATTGATGAACAAGGCCGGTGGAAATACGTGCGACTTCCACTCCGCTCGGCAAg < 7:55223/149‑1 (MQ=255) aGCAACTTCCCCGGTCAGCGCACCCTGTTCGGGTTGTACAGCAGAATTCTGTGCGCCGACAGTAATTTCTTTACCTGCCAGGCCATCAATCACCTGATTGATGAACAAGGCCGGTGGAAATACG‑GCGACTTCCACTCCGCTCGGCAAgg > 8:365562/1‑149 (MQ=255) aaCTTCCCCGGTCAGCGCACCCTGTTCGGGTTGTACAGCAGAATTCTGTGCGCCGACAGTAATTTCTTTACCTGCCAGGCCATCAATCACCTGATTGATGAACAAGGCCGGTGG‑AATACG‑GCGACTTCCACTCCGCTCGGCAAGGCgag < 3:376847/149‑1 (MQ=255) cTTCCCCGGTCAGCGCACCCTGTTCGGGTTGTACAGCAGAATTCTGTGCGCCGACAGTAATTTCTTTACCTGCCAGGCCATCAATCACCTGATTGATGAACAAGGCCGGTGGAAATACG‑GCGACTTCCACTCCGCTCGGCAAGGc > 1:238209/1‑145 (MQ=255) ttCCCCGGTCAGCGCACCCTGTTCGGGTTGTACAGCAGAATTCTGTGCGCCGACAGTAATTTCTTTACCTGCCAGGCCATCAATCACCTGATTGATGAACAAGGCCGGTGGAAATACG‑GCGACTTCCACTCCGCTCGGCAAGGCGAGAt > 2:356230/1‑149 (MQ=255) cccGGTCAGCGCACCCTGTTCGGGTTGTACAGCAGAATTCTGTGCGCCGACAGTAATTTCTTTACCTGCCAGGCCATCAATCACCTGATTGATGAACAAGGCCGGTGGAAATACG‑GCGACTTCCa > 8:204688/1‑125 (MQ=255) cccGGTCAGCGCACCCTGTTCGGGTTGTACAGCAGAATTCTGTGCGCCGACAGTAATTTCTTTACCTGCCAGGCCATCAATCACCTGATTGATGAACAAGGCCGGTGGAAATACG‑GCGACTTCCa < 7:204688/125‑1 (MQ=255) cccGGTCAGCGCACCCTGTTCGGGTTGTACAGCAGAATTCTGTGCGCCGACAGTAATTTCTTTACCTGCCAGGCCATCAATCACCTGATTGATGAACAAGGCCGGTGGAAATACG‑GCGACTTCCACTCCGCTCGGCAAGGCGAGATTa < 7:276886/148‑1 (MQ=255) cGGTCAGCGCACCCTGTTCGGGTTGTACAGCAGAATTCTGTGCGCCGACAGTAATTTCTTTACCTGCCAGGCCATCAATCACCTGATTGATGAACAAGGCCGGTGGAAATACG‑GCGACTTCCACTCCGCTCGGCAAGGCGAGATTACt > 4:72131/1‑148 (MQ=255) ttccgcgcACGCTGTTCGGGGTGTACAGAAGAATTCTGTGCGCCGACAGTAATTTCTTTACCTGCCAGGCCATCAATCACCTGATTGATGAACAAGGGCGGTGGAAATACG‑GCGACTTCCACTCCGCTCGGCAAGGCGAGATTACTCaa < 4:326720/145‑1 (MQ=255) aGCGCACCCTGTTCGGGTTGTACAGCAGAATTCTGTGCGCCGACAGTAATTTCTTTACCTGCCAGGCCATCAATCACCTGATTGATGAACAAGGCCGGTGGAAATACG‑GCGACTTCCACTCCGCTCGGCAAGGCGAGATTACTCAAGc < 6:21702/148‑1 (MQ=255) gcgcACCCTGTTCGGGTTGTACAGCAGAATTCTGTGCGCCGACAGTAATTTCTTTACCTGCCAGGCCATCAATCACCTGATTGATGAACAAGGCCGGTGGAAATACG‑GCGACTTCCACTCCGCTCGGCAAGGCGAGATTACTCAAGCCt < 7:365562/149‑1 (MQ=255) gcACCCTGTTCGGGTTGTACAGCAGAATTCTGTGCGCCGACAGTAATTTCTTTACCTGCCAGGCCATCAATCACCTGATTGATGAACAAGGCCGGTGGAAATACG‑GCGACTTCCACTCCGCTCGGCAAGGCGAGATTACTCAAGCCttt < 6:293291/149‑1 (MQ=255) cACCCTGTTCGGGTTGTACAGCAGAATTCTGTGCGCCGACAGTAATTTCTTTACCTGCCAGGCCATCAATCACCTGATTGATGAACAAGGCCGGTGGAAATACG‑GCGACTTCCACTCCGCTCGGCAAGGCGAGATTACTCAAGCCTTTg < 3:64524/149‑1 (MQ=255) cACCCTGTTCGGGTTGTACAGCAGAATTCTGTGCGCCGACAGTAATTTCTTTACCTGCCAGGCCATCAATCACCTGATTGATGAACAAGGCCGGTGGAAATACG‑GCGACTTCCACTCCGCTCGGCAAGGCGAGATTACTCAAGCCTTTg > 2:55968/1‑149 (MQ=255) ccTGTTCGGGTTGTACAGCAGAATTCTGTGCGCCGACAGTAATTTCTTTACCTGCCAGGCCATCAATCACCTGATTGATGAACAAGGCCGGTGGAAATACG‑GCGACTTCCACTCCGCTCGGCAAGGCGAGATTACTCAAGCCTTTg < 3:57703/146‑1 (MQ=255) ccTGTTCGGGTTGTACAGCAGAATTCTGTGCGCCGACAGTAATTTCTTTACCTGCCAGGCCATCAATCACCTGATTGATGAACAAGGCCGGTGGAAATACG‑GCGACTTCCACTCCGCTCGGCAAGGCGAGATTACTCAAGCCTTTg > 4:57703/1‑146 (MQ=255) ccTGTTCGGGTTGTACAGCAGAATTCTGTGCGCCGACAGTAATTTCTTTACCTGCCAGGCCATCAATCACCTGATTGATGAACAAGGCCGGTGGAAATACG‑GCGACTTCCACTCCGCTCGGCAAGGCGAGATTACTCAAGCCTTTGGt > 6:166412/1‑148 (MQ=255) ttCGGGTTGTACAGCAGAATTCTGTGCGCCGACAGTAATTTCTTTACCTGCCAGGCCATCAATCACCTGATTGATGAACAAGGCCGGTGGAAATACG‑GCGACTTCCACTCCGCTCGGCAAGGCGAGATTACTCAAGCCTTTGGtcag < 7:120648/147‑1 (MQ=255) ttCGGGTTGTACAGCAGAATTCTGTGCGCCGACAGTAATTTCTTTACCTGCCACGCCATCAATCACCTGATTGATGAACAAGGCCGGTGGAAATACG‑GCGACTTCCACTCCGCTCGGCAAGGCGAGATTACTCAAGCCTTTGGtcagct < 1:332880/149‑1 (MQ=255) gggTTGTACAGCAGAATTCTGTGCGCCGACAGTAATTTCTTTACCTGCCAGGCCATCAATCACCTGATTGATGAACAAGGCCGGTGGAAATACG‑GCGACTTCCACTCCGCTCGGCAAGGCGAGATTACTCAAGCCTTTGGtcagctca < 4:227688/148‑1 (MQ=255) gcagAATTCTGTGCGCCGACAGTAATTTCTTTACCTGCCAGGCCATCAATCACCTGATTGATGAACAAGGCCGGTGGAAATACG‑GCGACTTCCACTCCGCTCGGCAAGGCGAGATTACTCAAGCCTTTGGTCAGCTCAGCGACGCTAgc > 5:343527/1‑149 (MQ=255) cagAATTCTGTGCGCCGACAGTAATTTCTTTACCTGCCAGGCCATCAATCACCTGATTGATGAACAAGGCCGGTGGAAATACG‑GCGACTTCCACTCCGCTCGGCAAGGCGAGATTACTCAAGCCTTTGGTCAGCTCAGCGACGCTAgcg > 4:15212/1‑149 (MQ=255) cagAATTCTGTGCGCCGACAGTAATTTCTTTACCTGCCAGGCCATCAATCACCTGATTGATGAACAAGGCCGGTGGAAATACG‑GCGACTTCCACTCCGCTCGGCAAGGCGAGATTACTCAAGCCTTTGGTCAGCTCAGCGACGCTAgcg > 6:269506/1‑149 (MQ=255) agAATTCTGTGCGCCGACAGTAATTTCTTTACCTGCCAGGCCATCAATCACCTGATTGATGAACAAGGCCGGTGGAAATACG‑GCGACTTCCACTCCGCTCGGCAAGGCGAGATTACTCAAGCCTTTGGTCAGCTCAGCGACGCTAgcgc < 1:356230/149‑1 (MQ=255) tCTGTGCGCCGACAGTAATTTCTTTACCTGCCAGGCCATCAATCACCTGATTGATGAACAAGGCCGGTGGAAATACG‑GCGACTTCCACTCCGCTCGGCAAGGCGAGATTACTCAAGCCTTTGGTCAGCTCAGCGACGCTAGCGCGGGTa > 1:138594/1‑149 (MQ=255) tgCGCCGACAGTAATTTCTTTACCTGCCAGGCCATCAATCACCTGATTGATGAACAAGGCCGGTGGAAATACG‑GCGACTTCCACTCCGCTCGGCAAGGCGAGATTACTCAAGCCTTTGGTCAGCTCAGCGACGCTAGCGCGGGTACCGt > 6:224022/1‑149 (MQ=255) aCAGTAATTTCTTTACCTGCCAGGCCATCAATCACCTGATTGATGAACAAGGCCGGTGGAAATACG‑GCGACTTCCACTCCGCTCGGCAAGGCGAGATTACTCAAGCCTTTGGTCAGCTCAGCGACGCTAGCGCGGGTACCGTGCAtttt > 7:12810/1‑149 (MQ=255) cAGTAATTTCTTTACCTGCCAGGCCATCAATCACCTGATTGATGAACAAGGCCGGTGGAAATACG‑GCGACTTCCACTCCGCTCGGCAAGGCGAGATTACTCAAGCCTTTGGTCAGCTCAGCGACGCTAGCGCGGGTACCGTGCATTTTc < 2:179298/149‑1 (MQ=255) gTAATTTCTTTACCTGCCAGGCCATCAATCACCTGATTGATGAACAAGGCCGGTGGAAATACG‑GCGACTTCCACTCCGCTCGGCAAGGCGAGATTACTCAAGCCTTTGGTCAGCTCAGCGACGCTAGCGCGGGTACCGTGCATTTTCCa < 5:224022/149‑1 (MQ=255) gTAATTTCTTTACCTGCCAGGCCATCAATCACCTGATTGATGAACAAGGCCGGTGGAAATACG‑GCGACTTCCACTCCGCTCGGCAAGGCGAGATTACTCAAGCCTTTGGTCAGCTCAGCGACGCTAGCGCGGGTACCGTGCATTTTCCa < 3:72131/149‑1 (MQ=255) gTAATTTCTTTACCTGCCAGGCCATCAATCACCTGATTGATGAACAAGGCCGGTGGAAATACG‑GCGACTTCCACTCCGCTCGGCAAGGCGAGATTACTCAAGCCTTTGGTCAGCTCAGCGACGCTAGCGCGGGTACCGTGCATTTTCCa < 3:15212/149‑1 (MQ=255) gTAATTTCTTTACCTGCCAGGCCATCAATCACCTGATTGATGAACAAGGCCGGTGGAAATACG‑GCGACTTCCACTCCGCTCGGCAAGGCGAGATTACTCAAGCCTTTGGTCAGCTCAGCGACGCTAGCGCGGGTACCGTGCATTTTCCa < 1:55968/149‑1 (MQ=255) gTAATTTCTTTACCTGCCAGGCCATCAATCACCTGATTGATGAACAAGGCCGGTGGAAATACG‑GCGACTTCCACTCCGCTCGGCAAGGCGAGATTACTCAAGCCTTTGGTCAGCTCAGCGACGCTAGCGCGGGTACCGTGCATTTTCCa < 7:110718/149‑1 (MQ=255) tAATTTCTTTACCTGCCAGGCCATCAATCACCTGATTGATGAACAAGGCCGGTGGAAATACG‑GCGACTTCCACTCCGCTCGGCAAGGCGAGATTACTCAAGCCTTTGGTCAGCTCAGCGACGCTAGCGCGGGTACCGTGCATTTTCCAg > 6:287932/1‑149 (MQ=255) tACCTGCCAGGCCATCAATCACCTGATTGATGAACAAGGCCGGTGGAAATACG‑GCGACTTCCACTCCGCTCGGCAAGGCGAGATTACTCAAGCCTTTGGTCAGCTCAGCGACGCTAGCGCGGGTACCGTGCATTTTCCAGTTACCAGc > 2:379302/1‑148 (MQ=255) ccTGCCAGGCCATCAATCACCTGATTGATGAACAAGGCCGGTGGAAATACG‑GCGACTTCCACTCCGCTCGGCAAGGCGAGATTACTCAAGCCTTTGGTCAGCTCAGCGACGCTAGCGCGGGTACCGTGCATTTTCCAGTTACCAGCTAc < 8:22792/149‑1 (MQ=255) gCCAGGCCATCAATCACCTGATTGATGAACAAGGCCGGTGGAAATACG‑GCGACTTCCACTCCGCTCGGCAAGGCGAGATTACTCAAGCCTTTGGTCAGCTCAGCGACGCTAGCGCGGGTACCGTGCATTTTCCAGTTACCAGc > 1:15163/1‑143 (MQ=255) gCCAGGCCATCAATCACCTGATTGATGAACAAGGCCGGTGGAAATACG‑GCGACTTCCACTCCGCTCGGCAAGGCGAGATTACTCAAGCCTTTGGTCAGCTCAGCGACGCTAGCGCGGGTACCGTGCATTTTCCAGTTACCAGc < 2:15163/143‑1 (MQ=255) gCCAGGCCATCAATCACCTGATTGATGAACAAGGCCGGTGGAAATACG‑GCGACTTCCACTCCGCTCGGCAAGGCGAGATTACTCAAGCCTTTGGTCAGCTCAGCGACGCTAGCGCGGGTACCGTGCATTTTCCAGTTACCAGCTACCa > 5:75994/1‑148 (MQ=255) gCCAGGCCATCAATCACCTGATTGATGAACAAGGCCGGTGGAAATACG‑GCGACTTCCACTCCGCTCGGCAAGGCGAGATTACTCAAGCCTTTGGTCAGCTCAGCGACGCTAGCGCGGGTACCGTGCATTTTCCAGTTACCAGCTACCa > 2:356693/1‑148 (MQ=255) cttgttcATCAATCAGGTGATTGATGAACAAGGCCGGTGGAAATACG‑GCGACTTCCACTCCGCTCGGCAAGGCGAGATTACTCAAGCCTTTGGTCAGCTCAGCGACGCTAGCGCGGGTACCGTGCATTTTCCAGTTACCAGCTACCa > 7:55709/7‑147 (MQ=255) cAGGCCATCAATCACCTCATTGATGAACAAGGCCGGTGGAAATACG‑GCGACTTCCACTCCGCTCGGCAAGGCGAGATTACTCAAGCCTTTGGGCAGCTCAGCGACGCTAGCGCGGGTACCGTGCATTTTCCAGTTACCAGCTACCATAg < 4:52131/149‑1 (MQ=255) ggCCATCAATCACCTGATTGATGAACAAGGCCGGTGGAAATACG‑GCGACTTCCACTCCGCTCGGCAAGGCGAGATTACTCAAGCCTTTGGTCAGCTCAGCGACGCTAGCGCGGGTACCGTGCATTTTCCAGTTACCAGCTACCATAggg < 5:287932/149‑1 (MQ=255) gCCATCAATCACCTGATTGATGAACAAGGCCGGTGGAAATACG‑GCGACTTCCACTCCGCTCGGCAAGGCGAGATTACTCAAGCCTTTGGTCAGCTCAGCGACGCTAGCGCGGGTACCGTGCATTTTCCAGTTACCAGCTACCATAGGGc < 5:166412/149‑1 (MQ=255) gCCATCAATCACCTGATTGATGAACAAGGCCGGTGGAAATACG‑GCGACTTCCACTCCGCTCGGCAAGGCGAGATTACTCAAGCCTTTGGTCAGCTCAGCGACGCTAGCGCGGGTACCGTGCATTTTCCAGTTACCAGCTACCATAGGGc < 2:138594/149‑1 (MQ=255) tcATCAATCAGGTGATTGATGAACAAGGCCGGTGGAAATACG‑GCGACTTCCACTCCGCTCGGCAAGGCGAGATTACTCAAGCCTTTGGTCAGCTCAGCGACGCTAGCGCGGGTACCGTGCATTTTCCAGTTACGAGCTACCATAGGGc > 4:387657/2‑148 (MQ=255) atcaatcaCCTGATTGATGAACAAGGCCGGTGGAAATACG‑GCGACTTCCACTCCGCTCGGCAAGGCGAGATTACTCAAGCCTTTGGTCAGCTCAGCGACGCTAGCGCGGGTACCGTGCATTTTCCAGTTACCAGCTACCATAgg > 7:202392/1‑144 (MQ=255) atcaatcaCCTGATTGATGAACAAGGCCGGTGGAAATACG‑GCGACTTCCACTCCGCTCGGCAAGGCGAGATTACTCAAGCCTTTGGTCAGCTCAGCGACGCTAGCGCGGGTACCGTGCATTTTCCAGTTACCAGCTACCATAgg < 8:202392/144‑1 (MQ=255) atcaatcaCCTGATTGATGAACAAGGCCGGTGGAAATACG‑GCGACTTCCACTCCGCTCGGCAAGGCGAGATTACTCAAGCCTTTGGTCAGCTCAGCGACGCTAGCGCGGGTACCGTGCATTTTCCAGTTACCAGCTACCATAGGGCGAc < 3:401185/149‑1 (MQ=255) tcaCCTGATTGATGAACAAGGCCGGTGGAAATACG‑GCGACTTCCACTCCGCTCGGCAAGGCGAGATTACTCAAGCCTTTGGTCAGCTCAGCGACGCTAGCGCGGGTACCGTGCATTTTCCAGTTACCAGCTACCATAGGGCGACGCATg < 2:238209/149‑1 (MQ=255) aCCTGATTGATGAACAAGGCCGGTGGAAATACG‑GCGACTTCCACTCCGCTCGGCAAGGCGAGATTACTCAAGCCTTTGGTCAGCTCAGCGACGCTAGCGCGGGTACCGTGCATTTTCCAGTTACCAGCTACCATAGGGCGACGCATGc < 6:75994/148‑1 (MQ=255) aCCTGATTGATGAACAAGGCCGGTGGAAATACG‑GCGACTTCCACTCCGCTCGGCAAGGCGAGATTACTCAAGCCTTTGGTCAGCTCAGCGACGCTAGCGCGGGTACCGTGCATTTTCCAGTTACCAGCTACCATAGGGCGACGCATGCt < 8:12810/149‑1 (MQ=255) | CGCTCCGAATGGCCAATCAACACCAACTTGCAACCTGCTTCAACCAGCTGCTCGGGAGCAACTTCCCCGGTCAGCGCACCCTGTTCGGGTTGTACAGCAGAATTCTGTGCGCCGACAGTAATTTCTTTACCTGCCAGGCCATCAATCACCTGATTGATGAACAAGGCCGGTGGAAATACG‑GCGACTTCCACTCCGCTCGGCAAGGCGAGATTACTCAAGCCTTTGGTCAGCTCAGCGACGCTAGCGCGGGTACCGTGCATTTTCCAGTTACCAGCTACCATAGGGCGACGCATGCT > NC_002947/5362067‑5362362 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |