Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	421,161	T→A	5.6%	*929C (TGA→TGT)	PP_0348 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	421,161	0	T	A	5.6%	260.0 / 3.6	89	*929C (TGA→TGT)	PP_0348	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (42/41);  new base A (3/2);  total (45/44)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.21e-01

CCAGCACGCCACCCAGCTCAGCTTCGCCCCGGCCTATGCCCGTGACCGGGCACGGCTGCT‑GCGCAACTGGCCCACTGGCAGTGCCTTGCATCAGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAGCCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCAGCCACTGGCCGTTTTCGTGGCGCAGCACCGCCCACGGCACCACCCCGGCGTTGCTGGCAACGGCAATCTCCAAG  >  NC_002947/421014‑421308                                                                                                                                                     |                                                                                                                                                     ccAGCACGCCACCCAGCTCAGCTTCGCCCCGGCCTATGCCCGTGACCGGGCACGGCTGCT‑GCGCAACTGGCCCACTGGCAGTGCCTTGCATCAGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGt                                                                                                                                                      <  4:31014/148‑1 (MQ=255)  cAGCACGCCACCCAGCTCAGCTTCGCCCCGGCCTATGCCCGTGACCGGGCACGGCTGCT‑GCGCAACTGGCCCACTGGCAGTGCCTTGCATCAGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGt                                                                                                                                                      >  3:308280/1‑147 (MQ=255)  cAGCACGCCACCCAGCTCAGCTTCGCCCCGGCCTATGCCCGTGACCGGGCACGGCTGCT‑GCGCAACTGGCCCACTGGCAGTGCCTTGCATCAGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGt                                                                                                                                                      >  6:241028/1‑147 (MQ=255)  cAGCACGCCACCCAGCTCAGCTTCGCCCCGGCCTATGCCCGTGACCGGGCACGGCTGCT‑GCGCAACTGGCCCACTGGCAGTGCCTTGCATCAGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACGGAGGCCGCCGGCTCCGCTGTcc                                                                                                                                                    >  5:287811/1‑148 (MQ=255)    gCACGCCACCCATCTCAGCTTCGCCCCGGCCTATGCCCGTGACCGGGCACGGCTGCT‑GCGCAACTGGCCCACTGGCAGTGCCTTGCATCAGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGt                                                                                                                                                  <  7:104956/149‑1 (MQ=255)      aCGCCACCCAGCTCAGCTTCGCCCCGGCCTATGCCCGTGACCGGGCACGGCTGCT‑GCGCAACTGGCCCACTGGCAGTGCCTTGCATCAGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTgg                                                                                                                                                >  1:382836/1‑149 (MQ=255)           aCCCAGCTCAGCTTCGCCCCGGCCTATGCCCGTGACCGGGCACGGCTGCT‑GCGCAACTGGCCCACTGGCAGTGCCTTGCATCAGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCcgg                                                                                                                                          >  4:195993/1‑149 (MQ=255)           aCCCAGCTCAGCTTCGCCCCGGCCTATGCCCGTGACCGGGCACGGCTGCT‑GCGCAACTGGCCCACTGGCAGTGCCTTGCATCAGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCcgg                                                                                                                                          <  4:287004/149‑1 (MQ=255)           aCCCAGCTCAGCTTCGCCCCGGCCTATGCCCGTGACCGGGCACGGCTGCT‑GCGCAACTGGCCCACTGGCAGTGCCTTGCATCAGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCcgg                                                                                                                                          <  4:323608/149‑1 (MQ=255)             ccAGCTCAGCTTCGCCCCGGCCTATGCCCGTGACCGGGCACGGCTGCT‑GCGCAACTGGCCCACTGGCAGTGCCTTGCATCAGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCcggcg                                                                                                                                        <  5:386832/149‑1 (MQ=255)             ccAGCTCAGCTTCGCCCCGGCCTATGCCCGTGACCGGGCACGGCTGCT‑GCGCAACTGGCCCACTGGCAGTGCCTTGCATCAGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCcggcg                                                                                                                                        >  3:9183/1‑149 (MQ=255)              cagctcagctTCGCCCCGGCCTATGCCCGTGACCGGGCACGGCTGCT‑GCGCAACTGGCCCACTGGCAGTGCCTTGCATCAGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCcggcgg                                                                                                                                       >  6:303554/1‑149 (MQ=255)               agctcagctTCGCCCCGGCCTATGCCCGTGACCGGGCACGGCTGCT‑GCGCAACTGGCCCACTGGCAGTGCCTTGCATCAGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCggt                                                                                                                                      >  6:236610/1‑149 (MQ=255)                  tcagctTCGCCCCGGCCTATGCCCGTGACCGGGCACGGCTGCTGGCGCAACTGGCCCACTGGCAGTGCCTTGCATCAGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTg                                                                                                                                                 >  1:282557/1‑137 (MQ=255)                  tcagctTCGCCCCGGCCTATGCCCGTGACCGGGCACGGCTGCT‑GCGCAACTGGCCCACTGGCAGTGCCTTGCATCAGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTg                                                                                                                                                 <  2:282557/136‑1 (MQ=255)                        tCGCCCCGGCCTATGCCCGTGACCGGGCACGGCTGCT‑GCGCAACTGGCCCACTGGCAGTGCCTTGCATCAGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCt                                                                                                                             >  1:77074/1‑149 (MQ=255)                         cGCCCCGGCCTATGCCCGTGACCGGGCACGGCTGCT‑GCGCAACTGGCCCACTGGCAGTGCCTTGCATCAGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCt                                                                                                                             <  1:78767/148‑1 (MQ=255)                            cccGGCCTATGCCCGTGACCGGGCACGGCTGCT‑GCGCAACTGGCCCACTGGCAGTGCCTTGCATCAGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTgg                                                                                                                           >  3:254762/1‑147 (MQ=255)                            cccGGCCTATGCCCGTGACCGGGCACGGCTGCT‑GCGCAACTGGCCCACTGGCAGTGCCTTGCATCAGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCa                                                                                                                         <  1:136468/149‑1 (MQ=255)                              cGGCCTATGCCCGTGACCGGGCACGGCTGCT‑GCGCAACTGGCCCACTGGCAGTGCCTTGCATCAGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAgt                                                                                                                       <  4:308280/149‑1 (MQ=255)                              cGGCCTATGCCCGTGACCGGGCACGGCTGCT‑GCGCAACTGGCCCACTGGCAGTGCCTTGCATCAGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAgt                                                                                                                       <  7:327416/149‑1 (MQ=255)                                 ccTATGCCCGTGACCGGGCACGGCTGCT‑GCGCAACTGGCCCACTGGCAGTGCCTTGCATCAGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTc                                                                                                                    <  4:156667/149‑1 (MQ=255)                                 ccTATGCCCGTGACCGGGCACGGCTGCT‑GCGCAACTGGCCCACTGGCAGTGCCTTGCATCAGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTc                                                                                                                    >  1:153577/1‑149 (MQ=255)                                     tGCCCGTGACCGGGCACGGCTGCT‑GCGCATCTGGCCCACTGGCAGTGCCTTGCATCAGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAGc                                                                                                                 <  1:391529/148‑1 (MQ=255)                                       cccGTGACCGGGCACGGCTGCT‑GCGCAACTGGCCCACTGGCAGTGCCTTGCATCAGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAGCCAg                                                                                                              >  8:315922/1‑149 (MQ=255)                                       cccGTGACCGGGCACGGCTGCT‑GCGCAACTGGCCCACTGGCAGTGCCTTGCATCAGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGC‑CCACTGACAGCGGAGCcggcg                                                                                                                                        >  6:205702/1‑123 (MQ=255)                                       cccGTGACCGGGCACGGCTGCT‑GCGCAACTGGCCCACTGGCAGTGCCTTGCATCAGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGC‑CCACTGACAGCGGAGCcggcg                                                                                                                                        <  5:205702/123‑1 (MQ=255)                                           tGACCGGGCACGGCTGCT‑GCGCAACTGGCCCACTGGCAGTGCCTTGCATCAGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAGCCAGTCAc                                                                                                          <  7:83425/149‑1 (MQ=255)                                             aCCGGGCACGGCTGCT‑GCGCAACTGGCCCACTGGCAGTGCCTTGCATCAGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAGCCAGTCAct                                                                                                         <  7:350464/148‑1 (MQ=255)                                              ccGGGCACGGCTGCT‑GCGCAACTGGCCCACTGGCAGTGCCTTGCATCAGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAGCCAGTCActgc                                                                                                       >  5:253826/1‑149 (MQ=255)                                               cGGGCACGGCTGCT‑GCGCAACTGGCCCACTGGCAGTGCCTTGCATCAGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAGCCAGTCActgct                                                                                                      <  8:157746/149‑1 (MQ=255)                                                   cACGGCTGCT‑GCGCAACTGGCCCACTGGCAGTGCCTTGCATCAGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAGCCAGTCACTGCTgcgc                                                                                                  <  2:153577/149‑1 (MQ=255)                                                    aCGGCTGCT‑GCGCAACTGGCCCACTGGCAGTGCCTTGCATCAGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGAGCTGCCAGGGACAGCCAGGCACTGATGcccc                                                                                                 >  6:84785/1‑149 (MQ=255)                                                    aCGGCTGCT‑GCGCAACTGGCCCACTGGCAGTGCCTTGCATCAGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGACCGCCGGC‑CCACTGACAGCGGAGCcggcggc                                                                                                                                      <  3:138122/112‑2 (MQ=37)                                                    aCGGCTGCT‑GCGCAACTGGCCCACTGGCAGTGCCTTGCATCAGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGACCGCCGGC‑CCACTGACAGCGGAGCcggcggc                                                                                                                                      >  4:138122/1‑111 (MQ=37)                                                           ct‑gcGCAACTGGCCCACTGGCAGTGCCTTGCATCAGCGGCGGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGGCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAGCCAGTCACTGCTGCGCcctgcct                                                                                           <  2:339426/148‑1 (MQ=255)                                                            t‑gcGCAACTGGCCCACTGGCAGTGCCTTGCATCAGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAGCCAGTCACTGCTGCGCCCTGCCTgg                                                                                         >  1:246130/1‑149 (MQ=255)                                                              gcgcAACTGGCCCACTGGCAGTGCCTTGCATCAGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAGCCAGTCACTGCTGCGCCCTGCCTGGc                                                                                        <  7:315922/149‑1 (MQ=255)                                                                  aaCTGGCCCACTGGCAGTGCCTTGCATCAGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAGCCAGTCACTGCTGCGCCCTGCCTGGccagt                                                                                    <  2:146830/149‑5 (MQ=255)                                                                    cTGGCCCACTGGCAGTGCCTTGCAGCAGCGGCTGGACCGGCTGTTGCCAAAACTGGGCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAGCCAGTCACTGCTGCGCCCTGCCTGGCGAAcgc                                                                                  <  4:254762/149‑1 (MQ=255)                                                                     tGGCCCACTGGCAGTGCCTTGCATCAGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAGCCAGTCACTGCTGCGCCCTGCCTGGCGAAcgcg                                                                                 >  4:15158/1‑149 (MQ=255)                                                                         ccACTGGCAGTGCCTTGCATCAGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAGCCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTAt                                                                              >  4:124728/1‑148 (MQ=255)                                                                          cACTGGCAGTGCCTTGCATCAGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAGCCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCa                                                                            <  4:9183/149‑1 (MQ=255)                                                                            cTGGCAGTGCCTTGCATCAGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAGCCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCAGc                                                                          >  2:183738/1‑149 (MQ=255)                                                                                cAGTGCCTTGCATCAGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAGCCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCAGCCACt                                                                      >  1:258337/1‑149 (MQ=255)                                                                                 aGTGCCTTGCATCAGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGACTGGCTGACActgcca                                                                                                     >  6:378783/1‑115 (MQ=255)                                                                                 aGTGCCTTGCATCAGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGACTGGCTGACActgcca                                                                                                     <  5:378783/117‑3 (MQ=255)                                                                                     ccTTGCATCAGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAGCCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCAGCCACTGGCCg                                                                 >  1:361754/1‑149 (MQ=255)                                                                                         gCATCAGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCGGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAGCCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCAGCCACTGGCCGttt                                                              <  7:136073/148‑1 (MQ=255)                                                                                         gCATCAGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAGCCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCAGCCACTGGCCGtttt                                                             >  1:79882/1‑149 (MQ=255)                                                                                           aTCAGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAGCCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCAGCCACTGGCCGTTTTCg                                                           >  1:370411/1‑149 (MQ=255)                                                                                            tCAGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAGCCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCAGCCACTGGCCGTTTTCGt                                                          >  2:151802/1‑149 (MQ=255)                                                                                            tCAGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAGCCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCAGCCACTGGCCGTTTTCGt                                                          >  3:136506/1‑149 (MQ=255)                                                                                              aGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAGCCAGTCACTGCTGCGCCCTGCCTTGCGAACGCGTATCAGCCACTGGCCGTTTTCGTgg                                                        <  2:387909/149‑1 (MQ=255)                                                                                               gCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAGCCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCAGCCACTGGCCGTTTTCGTGgc                                                       <  1:183738/149‑1 (MQ=255)                                                                                               gCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAGCCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCAGCCACTGGCCGTTTTCGTGgc                                                       >  8:141195/1‑149 (MQ=255)                                                                                                    tGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAGCCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCAGCCACTGGCCGTTTTCGTGGCgcagc                                                  >  2:387058/1‑149 (MQ=255)                                                                                                      gACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAGCCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCAGCCACTGGCCGTTTTCGTGGCgcagca                                                 <  1:20803/148‑1 (MQ=255)                                                                                                        ccGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAGCCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCAGCCACTGGcc                                                                  <  4:84711/129‑1 (MQ=255)                                                                                                        ccGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAGCCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCAGCCACTGGcc                                                                  >  3:84711/1‑129 (MQ=255)                                                                                                        ccGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAGCCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCAGCCACTGGCCGTTTTCGTGGCGCAGCAcca                                              <  3:360487/149‑2 (MQ=255)                                                                                                          ggCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAGCCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCAGCCACTGGCCGTTTTCGTGGCGCAGCACCGcc                                            <  3:124728/149‑1 (MQ=255)                                                                                                                 gCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAGCCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCAGCCACTGGCCGTTTTCGTGGCGCAGCACCGCCCACGGca                                     >  4:362826/1‑149 (MQ=255)                                                                                                                 gCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAGCCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCAGCCACTGGCCGTTTTCGTGGCGCAGCACCGCCCACGGca                                     >  3:194557/1‑149 (MQ=255)                                                                                                                  ccAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAGCCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCAGCCACTGGCCGTTTTCGTGGCGCAGCACCGCCCACGGcac                                    >  8:111531/1‑149 (MQ=255)                                                                                                                  ccAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAGCCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCAGCCACTGGCCGTTTTCGTGGCGCAGCACCGCCCACGGcac                                    <  2:382836/149‑1 (MQ=255)                                                                                                                   cAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAGCCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCAGCCACTGGCCGTTTTCGTGGCGCAGCACCGCCCACGGcacc                                   >  8:53296/1‑149 (MQ=255)                                                                                                                   cAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAGCCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCAGCCACTGGCCGTTTTCGTGGCGCAGCACCGCCCACGGcacc                                   >  2:41561/1‑149 (MQ=255)                                                                                                                   cAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAGCCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCAGCCACTGGCCGTTTTCGTGGCGCAGCACCGCCCACGGcac                                    >  4:147304/1‑148 (MQ=255)                                                                                                                    aaaaCTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAGCCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCAGCCACTGGCCGTTTTCGTGGCGCAGCACCGCCCACGGcacca                                  >  8:128097/1‑149 (MQ=255)                                                                                                                    aaaaCTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAGCCAGTCACTGCTGCGCCCTGCCTGACGAACGCGTATCAGCCACTGGCCGTTTTCGTGGCGCAGCACCGCCCACGGcacca                                  >  6:415376/1‑149 (MQ=255)                                                                                                                           gTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAGCCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCAGCCACTGGCCGTTTTCGTGGCGCAGCACCGCCCACGGCACCACcgcg                             <  6:287811/147‑1 (MQ=255)                                                                                                                           gTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAGCCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCAGCCACTGGCCGTTTTCGTGGCGCAGCACCGCCCACGGCACCACCCCGGc                           <  3:362826/149‑1 (MQ=255)                                                                                                                            tCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAGCCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCAGCCACTGGCCGTTTTCGTGGCGCAGCACCGCCCACGGCACCACCCCGGCg                          >  5:360153/1‑149 (MQ=255)                                                                                                                              gACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAGCCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCAGCCACTGGCCGTTTTCGTGGCGCAGCACCGCCCACGGCACCACCCCGGCGt                         <  2:258337/148‑1 (MQ=255)                                                                                                                              gACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCACTGTCAGCCAGTCACTGCTGCGCCCTGCCTGACGAACGCGTATCAGCCACTGGCCGTTTTCGTGGCGCAGCACCGCCCACGGCACCACCCCGGCGt                         <  5:415376/148‑1 (MQ=255)                                                                                                                               aCTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAGCCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCAGCCACTGGCCGTTTTCGTGGCGCAGCACCGCCCACGGCACCACCCCGGCGTTg                       <  5:303554/149‑1 (MQ=255)                                                                                                                                cTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAGCCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCAGCCACTGGCCGTTTTCGTGGCGCAGCACCGCCCACGGCACCACCCCGGCGTTGc                      <  3:269327/149‑1 (MQ=255)                                                                                                                                 tGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAGCCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCAGCCACTGGCCGTTTTCGTGGCGCAGCACCGCCCACGGCACCACCCCGGCGTTGCt                     >  8:41074/1‑149 (MQ=255)                                                                                                                                   aGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAGCCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCAGCCACTGGCCGTTTTCGTGGCGCAGCACCGCCCACGGCACCACCCCGGCGTTGCt                     <  2:77074/147‑1 (MQ=255)                                                                                                                                   aGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAGCCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCAGCCACTGGCCGTTTTCGTGGCGCAGCACCGCCCACGGCACCACCCCGGCGTTGCTg                    <  2:246130/148‑1 (MQ=255)                                                                                                                                   aGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAGCCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCAGCCACTGGCCGTTTTCGTGGCGCAGCACCGCCCACGGCACCACCCCGGCGTTGCTg                    <  5:236610/148‑1 (MQ=255)                                                                                                                                    ggCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAGCCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCAGCCACTGGCCGTTTTCGTGGCGCAGCACCGCCCACGGCACCACCCCGGCGTTGCTGGc                  <  7:128097/149‑1 (MQ=255)                                                                                                                                    caccgccgGC‑CCCCTGACAGCGGAGCCGGCGGTGGTTGATCTGGCAGTGTCAGCCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCAGCCACTGGCCGTTTTCGTGGCGCAGCACCGCCCACGGCACCACCCCGGCGTTGCTgg                   >  1:257650/3‑148 (MQ=255)                                                                                                                                       cgccgGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAGCCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCAGCCACTGGCCGTTTTCGTGGCGCAGCACCGCCCACGGCACCACCCCGGCGTTGCTGGCAAc               >  5:335999/1‑149 (MQ=255)                                                                                                                                       cgccgGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAGCCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCAGCCACTGGCCGTTTTCGTGGCGCAGCACCGCCCACGGCACCACCCCGGCGTTGCTGGCAAc               <  6:253826/149‑1 (MQ=255)                                                                                                                                              tCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAGCCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCAGCCACTGGCCGTTTTCGTGGCGCAGCACCGCCCACGGCACCACCCCGGCGTTGCTGGCAACgg             <  7:182834/144‑1 (MQ=255)                                                                                                                                              tCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAGCCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCAGCCACTGGCCGTTTTCGTGGCGCAGCACCGCCCACGGCACCACCCCGGCGTTGCTGGCAACgg             >  8:182834/1‑144 (MQ=255)                                                                                                                                              tCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAGCCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCAGCCACTGGCCGTTTTCGTGGCGCAGCACCGCCCACGGCACCACCCCGGCGTTGCTGGCAACGGCAAtc        >  2:315229/1‑149 (MQ=255)                                                                                                                                                cGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAGCCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCAGCCACTGGCCGTTTTCGTGGCGCAGCACCGCCCACGGCACCACCCCGGCGTTGCTGGCAACGGCAAtctc      >  7:152724/1‑149 (MQ=255)                                                                                                                                                cGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAGCCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCAGCCACTGGCCGTTTTCGTGGCGCAGCACCGCCCACGGCACCACCCCGGCGTTGCTGGCAACGGCAAtctc      >  6:15682/1‑149 (MQ=255)                                                                                                                                                   tGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAGCCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCAGCCACTGGCCGTTTTCGTGGCGCAGCACCGCCCACGGCACCACCCCGGCGTTGCTGGCAACGGCAATCTCCaa   <  2:370411/149‑1 (MQ=255)                                                                                                                                                     tCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAGCCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCAGCCACTGGCCGTTTTCGTGGCGCAGCACCGCCTACGGCACCACCCCGGCGTTGCTGGCAACGGCAATCTCCAAg  <  2:79882/148‑1 (MQ=255)                                                                                                                                                     tCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAGCCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCAGCCACTGGCCGTTTTCGTGGCGCAGCACCGCCCACGGCACCACCCCGGCGTTGCTGGCAACGGCAATCTCCaa   <  5:241028/147‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                     CCAGCACGCCACCCAGCTCAGCTTCGCCCCGGCCTATGCCCGTGACCGGGCACGGCTGCT‑GCGCAACTGGCCCACTGGCAGTGCCTTGCATCAGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAGCCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCAGCCACTGGCCGTTTTCGTGGCGCAGCACCGCCCACGGCACCACCCCGGCGTTGCTGGCAACGGCAATCTCCAAG  >  NC_002947/421014‑421308

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑