Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,383,308 A→T 15.9% intergenic (‑28/‑229) pdhR ← / → lldP DNA‑binding transcriptional regulator LldR/L‑lactate permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,383,3080AT15.9% 115.2 / 10.4 50intergenic (‑28/‑229)pdhR/lldPDNA‑binding transcriptional regulator LldR/L‑lactate permease
Reads supporting (aligned to +/- strand):  ref base A (19/23);  new base T (4/4);  total (23/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAGGACGCGCTCGGCCGGCAGCCGCTGCCCCGAGGTCAGCGTGCCTTCCAGAATCATCCCTTCCAGCCGATCGACAATGTCGTCGGACAGGCGCCGTTGGCGTACCTGATCAAAAACCATCACATGCTCTCCACAAACCCCCGGCAAATTTCAGGGGGTGCCTATTCTCGCCGATCCAGGCCACGCAAACACCCATCAAGCCGCGATTTTCCCAGCACATCAGATGCCCGCTCATCGGTACGCGACCAAAGTTTTGCACAGGACAAATTGACACACCCACGGCAGCGCTTTTAAC  >  NC_002947/5383161‑5383455
                                                                                                                                                   |                                                                                                                                                   
cAGGACGCGCTCGGCCGGCAGCCGCTGCCCCGAGGTCAGCGTGCCTTCCAGAATCATCCCTTCCAGCCGATCGACAATGTCGTCGGACAGGCGCCGTTGGCGTACCTGATCAAAAACCATCACATGCTCTCCACAAACCCCCGGCAAAt                                                                                                                                                    >  5:162259/1‑149 (MQ=255)
      gcgcTCGGCCGGCAGCCGCTGCCCCGAGGTCAGCGTGCCTTCCAGAATCATCCCTTCCAGCCGATCGACAATGTCGTCGGACAGGCGCCGTTGGCGTACCTGATCAAAAACCATCACATGCTCTCCACAAACCCCCGGCAAATTTCAgg                                                                                                                                              >  4:202761/1‑149 (MQ=255)
          tCGGCCGGCAGCCGCTGCCCCGAGGTCAGCGTGCCTTCCAGAATCATCCCTTCCAGCCGATCGACAATGTCGTCGGACAGGCGCCGTTGGCGTACCTGATCAAAAACCATCACATGCTCTCCACAAACCCCCTGAAATTTGCCGGGGGt                                                                                                                                          >  3:270915/1‑149 (MQ=255)
            ggccggcAGCCGCTGCCCCGAGGTCAGCGTGCCTTCCAGAATCATCCCTTCCAGCCGATCGACAATGTCGTCGGACAGGCGCCGTTGGCGTACCTGATCAAAAACCATCACATGCTCTCCACAAACCCCCTGAAATTTGCCGGGGGt                                                                                                                                          <  2:390101/147‑1 (MQ=255)
            ggccggcAGCCGCTGCCCCGAGGTCAGCGTGCCTTCCAGAATCATCCCTTCCAGCCGATCGACAATGTCGTCGGACAGGCGCCGTTGGCGTACCTGATCAAAAACCATCACATGCTCTCCACAAACCCCCGGCAAATTTCAGGGGGt                                                                                                                                          <  8:312572/147‑1 (MQ=255)
             gccggcAGCCGCTGCCCCGAGGTCAGCGTGCCTTCCAGAATCATCCCTTCCAGCCGATCGACCATGTCGTCGGACAGGCGCCGTTGGCGTACCTGATCAAAACCCATCACATGCTCTCCACACACCCCCGGCAATTTTCAGGGGGTGcg                                                                                                                                       >  4:221233/1‑148 (MQ=255)
              ccggcAGCCGCTGCCCCGAGGTCAGCGTGCCTTCCAGAATCATCCCTTCCAGCCGATCGACAATGTCGTCGGACAGGCGCCGTTGGCGTACCTGATCAAAAACCATCACATGCTCTCCACAAACCCCCTGAAATTTGCCGGGGGtttgt                                                                                                                                      <  6:84749/149‑5 (MQ=255)
               cggcAGCCGCTGCCCCGAGGTCAGCGTGCCTTCCAGAATCATCCCTTCCAGCCGATCGACAATGTCGTCGGACAGGCGCCGTTGGCGTACCTGATCAAAAACCATCACATGCTCTCCACAAACCCCCTGAAATTTGCCGGGGGtttgtg                                                                                                                                     <  4:270915/149‑6 (MQ=255)
               cggcAGCCGCTGCCCCGAGGTCAGCGTGCCTTCCAGAATCATCCCTTCCAGCCGATCGACAATGTCGTCGGACAGGCGCCGTTGGCGTACCTGAACAAAAACCATCACATGCTCTCCACAAACCCCCTGAAATTTGCCGGGGGtttgtg                                                                                                                                     <  8:290364/149‑6 (MQ=255)
                ggcAGCCGCTGCCCCGAGGTCAGCGTGCCTTCCAGAATCATCCCTTCCAGCCGATCGACAATGTCGTCGGACAGGCGCCGTTGGCGTACCTGATCAAAAACCATCACATGCTCTCCACAAACCCCCGGCAAATTTCAGGGGGTGCCTAt                                                                                                                                    >  2:118834/1‑149 (MQ=255)
                       gCTGCCCCGAGGTCAGCGTGCCTTCCAGAATCATCCCTTCCAGCCGATCGACAATGTCGTCGGACAGGCGCCGTTGGCGTACCTGATCAAAAACCATCACATGCTCTCCACAAACCCCCGGCAAATTTCAGGGGGTGCCTATTCTCGcc                                                                                                                             <  6:219813/149‑1 (MQ=255)
                        cTGCCCCGAGGTCAGCGTGCCTTCCAGAATCATCCCTTCCAGCCGATCGACAATGTCGTCGGACAGGCGCCGTTGGCGTACCTGATCAAAAACCATCACATGCTCTCCACAAACCCCCGGCAAATTTCAGGGGGTGCCTATTCTCGCCg                                                                                                                            <  1:118834/149‑1 (MQ=255)
                              cGAGGTCAGCGTGCCTTCCAGAATCATCCCTTCCAGCCGATCGACAATGTCGTCGGACAGGCGCCGTTGGCGTACCTGATCAAAAACCATCACATGCTCTCCACAAACCCCCGGCAAATTTCAGGGGGTGCCTATTCTCGCCGATCCAg                                                                                                                      <  4:38720/149‑1 (MQ=255)
                               gAGGTCAGCGTGCCTTCCAGAATCATCCCTTCCAGCCGATCGACAATGTCGTCGGACAGGCGCCGTTGGCGTACCTGATCAAAAACCATCACATGCTCTCCACAAACCCCCGGCAAATTTCAGGGGGTGCCTATTCTCGCCGATCCAgg                                                                                                                     >  8:150336/1‑149 (MQ=255)
                                           ccTTCCAGAATCATCCCTTCCAGCCGATCGACAATGTCGTCGGACAGGCGCCGTTGGCGTACCTGATCAAAAACCATCACATGCTCTCCACAAACCCCCGGCAAATTTCAGGGGGTGCCTATTCTCGCCGATCCAGGCCACGCAAaca                                                                                                          <  6:182132/148‑1 (MQ=255)
                                           ccTTCCAGAATCATCCCTTCCAGCCGATCGACAATGTCGTCGGACAGGCGCCGTTGGCGTACCTGATCAAAAACCATCACATGCTCTCCACAAACCCCCGGCAAATTTCAGGGGGTGCCTATTCTCGCCGATCCAGGCCACGCAAaca                                                                                                          <  6:162259/148‑1 (MQ=255)
                                            cTTCCAGAATCATCCCTTCCAGCCGATCGACAATGTCGTCGGACAGGCGCCGTTGGCGTACCTGATCAAAAACCATCACATGCTCTCCACAAACCCCCGGCAAATTTCAGGGGGTGCCTATTCTCGCCGATCCAGGCCACGCAAACAcc                                                                                                        <  7:231633/149‑1 (MQ=255)
                                            cTTCCAGAATCATCCCTTCCAGCCGATCGACAATGTCGTCGGACAGGCGCCGTTGGCGTACCTGATCAAAAACCATCACATGCTCTCCACAAACCCCCGGCAAATTTCAGGGGGTGCCTATTCTCGCCGATCCAGGCCACGCAAACAcc                                                                                                        >  1:326267/1‑149 (MQ=255)
                                               ccAGAATCATCCCTTCCAGCCGATCGACAATGTCGTCGGACAGGCGCCGTTGGCGTACCTGATCAAAAACCATCACATGCTCTCCACAAACCCCCGGCAAATTTCAGGGGGTGCCTATTCTCGCCGATCCAGGCCACGCAAACACCCa                                                                                                      <  1:236588/148‑1 (MQ=255)
                                                       atcCCTTCCAGCCGATCGACAATGTCGTCGGACAGGCGCCGTTGGCGTACCTGATCAAAAACCATCACATGCTCTCCACAAACCCCCGGCAAATTTCAGGGGGTGCCTATTCTCGCCGATCCAGGCCACGCAAACACCCATCAAGCcgc                                                                                             >  1:2087/1‑149 (MQ=255)
                                                       atcCCTTCCAGCCGATCGACAATGTCGTCGGACAGGCGCCGTTGGCGTACCTGATCAAAAACCATCACATGCTCTCCACAAACCCCCGGCAAATTTCAGGGGGTGCCTATTCTCGCCGATCCAGGCCACGCAAACACCCATCAAGCcgc                                                                                             <  5:82345/149‑1 (MQ=255)
                                                        tcCCTTCCAGCCGATCGACAATGTCGTCGGACAGGCGCCGTTGGCGTACCTGATCAAAAACCATCACATGCTCTCCACAAACCCCCGGCAAATTTCAGGGGGTGCCTATTCTCGCCGATCCAGGCCACGCAAACACCCATCAAGCcgc                                                                                             >  1:3049/1‑148 (MQ=255)
                                                              ccAGCCGATCGACAATGTCGTCGGACAGGCGCCGTTGGCGTACCTGATCAAAAACCATCACATGCTCTCCACAAACCCCCGGCAAATTTCAGGGGGTGCCTATTCTCGCCGATCCAGGCCACGCAAACACCCATCAAGCCGCGATTTTc                                                                                      >  5:26231/1‑149 (MQ=255)
                                                               cAGCCGATCGACAATGTCGTCGGACAGGCGCCGTTGGCGTACCTGATCAAAAACCATCACATGCTCTCCACAAACCCCCGGCAAATTTCAGGGGGTGCCTATTCTCGCCGATCCAGGCCACGCAAACACCCATCAAGCCGCGATTTTcc                                                                                     <  5:327794/149‑1 (MQ=255)
                                                                 gCCGATCGACAATGTCGTCGGACAGGCGCCGTTGGCGTACCTGATCAAAAACCATCACATGCTCTCCACAAACCCCCGGCAAATTTCAGGGGGTGCCTATTCTCGCCGATCCAGGCCACGCAAACACCCATCAAGCCGCGATTTTCCCa                                                                                   <  3:148968/149‑1 (MQ=255)
                                                                    gATCGACAATGTCGTCGGACAGGCGCCGTTGGCGTACCTGATCAAAAACCATCACATGCTCTCCACAAACCCCCGGCAAATTTCAGGGGGTGCCTATTCTCGCCGATCCAGGCCACGCAAACACCCATCaa                                                                                                  <  4:221473/131‑1 (MQ=255)
                                                                    gATCGACAATGTCGTCGGACAGGCGCCGTTGGCGTACCTGATCAAAAACCATCACATGCTCTCCACAAACCCCCGGCAAATTTCAGGGGGTGCCTATTCTCGCCGATCCAGGCCACGCAAACACCCATCaa                                                                                                  >  3:221473/1‑131 (MQ=255)
                                                                      tCGACAATGTCGTCGGACAGGCGCCGTTGGCGTACCTGATCAAAAACCATCACATGCTCTCCACAAACCCCCGGCAAATTTCAGGGGGTGCCTATTCTCGcc                                                                                                                             >  5:5729/1‑102 (MQ=255)
                                                                      tCGACAATGTCGTCGGACAGGCGCCGTTGGCGTACCTGATCAAAAACCATCACATGCTCTCCACAAACCCCCGGCAAATTTCAGGGGGTGCCTATTCTCGcc                                                                                                                             <  6:5729/102‑1 (MQ=255)
                                                                        gACAATGTCGTCGGACAGGCGCCGTTGGCGTACCTGATCAAAAACCATCACATGCTCTCCACAAACCCCCGGCAAATTTCAGGGGGTGCCTATTCTCGCCGATCCAGGCCACGCAAACACCCATCAAGCCGCGATTTTCCCAGCACAt                                                                             >  3:22065/1‑148 (MQ=255)
                                                                         aCAATGTCGTCGGACAGGCGCCGTTGGCGTACCTGATCAAAAACCATCACATGCTCTCCACAAACCCCCGGCAAATTTCAGGGGGTGCCTATTCTCGCCGATCCAGGCCACGCAAACACCCATCAAGCCGCGATTTTCCCAGCACATCa                                                                           <  2:3049/149‑1 (MQ=255)
                                                                         aCAATGTCGTCGGACAGGCGCCGGTGGCGTACCTGATCAAAAACCATCACATGCTCTCCACAAACCCCCGGCAAATTTCAGGGGGTGCCTATTCTCGCCGATCCAGGCCACGCAAACACCCATCAAGCCGCGATTTTCCCAGCACATCa                                                                           <  4:22065/149‑1 (MQ=255)
                                                                                 gtcgGACAGGCGCCGTTGGCGTACCTGATCAAAAACCATCACATGCTCTCCACAAACCCCCGGCAATTTTCAGGGGGTGCCTATTCTCGCCGATCCAGGCCACGCAAACCCCCATCAAGCCGCGATTTACCCAGCACATCAGATGCCCg                                                                   >  5:267396/1‑149 (MQ=255)
                                                                                  tcgGACAGGCGCCGTTGGCGTACCTGATCAAAAACCATCACATGCTCTCCACAAACCCCCGGCAAATTTCAGGGGGTGCCTATTCTCGCCGATCCAGGCCACGCAAACACCCATCAAGCCGCGAt                                                                                          <  7:380322/125‑1 (MQ=255)
                                                                                  tcgGACAGGCGCCGTTGGCGTACCTGATCAAAAACCATCACATGCTCTCCACAAACCCCCGGCAAATTTCAGGGGGTGCCTATTCTCGCCGATCCAGGCCACGCAAACACCCATCAAGCCGCGAt                                                                                          >  8:380322/1‑125 (MQ=255)
                                                                                    ggACAGGCGCCGTTGGCGTACCTGATCAAAAACCATCACATGCTCTCCACAAACCCCCGGCAAATTTCAGGGGGTGCCTATTCTCGCCGATCCAGGCCACGCAAACACCCATCAAGCCGCGATTTTCCCAGCACATCAGATGCCCGCt                                                                 >  5:298577/1‑148 (MQ=255)
                                                                                              cGTTGGCGTACCTGATCAAAAACCATCACATGCTCTCCACAAACCCCCGGCAAATTTCAGGGGGTGCCTATTCTCGCCGCTCCAGGCCACGCACACACCCAGCAAGCCGCGATTTTCCCAGCCCTTCAGATGCCGGCTCATCGGTCcgc                                                      >  2:349701/1‑149 (MQ=255)
                                                                                                ttGGCGTACCTGATCAAAAACCATCACATGCTCTCCACAAACCCCCGGCAAATTTCAGGGGGTGCCTATTCTCGCCGATCCAGGCCACGCAAACACCCATCAAGCCGCGATTTTCCCAGCACATCAGATGCCCGCTCATCGGTACGCGa                                                    <  6:26231/149‑1 (MQ=255)
                                                                                                ttGGCGTACCTGATCAAAAACCATCACATGCTCTCCACAAACCCCCGGCAAATTTCAGGGGGTGCCTATTCTCGCCGATCCAGGCCACGCAAACACCCATCAAGCCGCGATTTTCCCAGCACATCAGATGCCCGCTCATCGGTACGCGa                                                    <  7:150336/149‑1 (MQ=255)
                                                                                                     gTACCTGATCAAAAACCATCACATGCTCTCCACAAACCCCCGGCAAATTTCAGGGGGTGCCTATTCTCGCCGATCCAGGCCACGCAAACACCCATCAAGCCGCGATTTTCCCAGCACATCAGATGCCCGCTCATCGGTACGCGACCaaa                                               <  5:226678/149‑1 (MQ=255)
                                                                                                      tACCTGATCAAAAACCATCACATGCTCTCCACAAACCCCCGGCAAATTTCAGGGGGTGCCTATTCTCGCCGATCCAGGCCACGCAAACACCCATCAAGCCGCGATTTTCCCAGCACATCAGATGCCCGCTCATCGGTACGCGACCAAAg                                              <  3:202761/149‑1 (MQ=255)
                                                                                                             tCAAAAACCATCACATGCTCTCCACAAACCCCCGGCAAATTTCAGGGGGTGCCTATTCTCGCCGATCCAGGCCACGCAAACACCCATCAAGCCGCGATTTTCCCAGCACATCAGATGCCCGCTCATCGGTACGCGACCAAAGTTTTGca                                       >  6:24107/1‑149 (MQ=255)
                                                                                                              cAAAAACCATCACATGCTCTCCACAAACCCCCGGCAAATTTCAGGGGGTGCCTATTCTCGCCGATCCAGGCCACGCAAACACCCATCAAGCCGCGATTTTCCCAGCACATc                                                                            <  5:162581/111‑1 (MQ=255)
                                                                                                              cAAAAACCATCACATGCTCTCCACAAACCCCCGGCAAATTTCAGGGGGTGCCTATTCTCGCCGATCCAGGCCACGCAAACACCCATCAAGCCGCGATTTTCCCAGCACATc                                                                            >  6:162581/1‑111 (MQ=255)
                                                                                                                           aTGCTCTCCACAAACCCCCGGCAAATTTCAGGGGGTGCCTATTCTCGCCGATCCAGGCCACGCAAACACCCATCAAGCCGCGATTTTCCCAGCACATCAGATGCCCGCTCATCGGTACGCGACCAAAGTTTTGCACAGGACAAATTGa                          >  8:41424/1‑148 (MQ=255)
                                                                                                                                     caAACCCCCGGCAAATTTCAGGGGGTGCCTATTCTCGCCGATCCAGGCCACGCAAACACCCATCAAGCCGCGATTTTCCCAGCACATCAGATGCCCGCTCATCGGTACGCGACCAAAGTTTTGCACAGGACAAATTGACACACCCACgg               >  3:366153/1‑149 (MQ=255)
                                                                                                                                        aCCCCCTGAAATTTGCCGGGGGTGCCTATTCTCGCCGATCCAGGCCACGCAAACACCCATCAAGCCGCGATTTTCCCAGCACATCAGATGCCCGCTAATCGGTACGCGCCAAAAGTTTTGCACAGGAAAAATTGACACACCCACGGCAg            >  4:64467/1‑149 (MQ=255)
                                                                                                                                             cGGCAAATTTCAGGGGGTGCCTATTCTCGCCGATCCAGGCCACGCAAACACCCATCAAGCCGCGATTTTCCCAGCACATCAGATGCCCGCTCATCGGTACGCGACCAAAGTTTTGCACAGGACAAATTGACACACCCACGGCAGCGCtt       <  2:2087/149‑1 (MQ=255)
                                                                                                                                              ggCAAATTTCAGGGGGTGCCTATTCTCGCCGATCCAGGCCACGCAAACACCCATCAAGCCGCGATTTTCCCAGCACATCAGATGCCCGCTCATCGGTACGCGACCAAAGTTTTGCACAGGACAAATTGACACACCCACGGCAGCGCttt      >  6:40374/1‑149 (MQ=255)
                                                                                                                                               gCAAATTTCAGTGTGTGCCTATTCTCGCCGATCCAGGCCAAGCAAACACCCATCAAGCCGCGATTTTCCCAGCACATCAGATGCCCGCTCATCGGTACGCGACCAAAGTTTTGCACAGGACAAATTGACACACCCACGGCAGCGCtttt     <  2:326267/149‑1 (MQ=255)
                                                                                                                                                  aaTTTCAGGGGGTGCCTATTCTCGCCGATCCAGGCCACGCAAACACCCATCAAGCCGCGATTTTCCCAGCACATCAGATGCCCGCTCATCGGTACGCGACCAAAGTTTTGCACAGGACAAATTGACACACCCACGGCAGCGCTTTTAAc  <  5:40374/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
CAGGACGCGCTCGGCCGGCAGCCGCTGCCCCGAGGTCAGCGTGCCTTCCAGAATCATCCCTTCCAGCCGATCGACAATGTCGTCGGACAGGCGCCGTTGGCGTACCTGATCAAAAACCATCACATGCTCTCCACAAACCCCCGGCAAATTTCAGGGGGTGCCTATTCTCGCCGATCCAGGCCACGCAAACACCCATCAAGCCGCGATTTTCCCAGCACATCAGATGCCCGCTCATCGGTACGCGACCAAAGTTTTGCACAGGACAAATTGACACACCCACGGCAGCGCTTTTAAC  >  NC_002947/5383161‑5383455

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑