BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,420,454	C→T	6.1%	P215P (CCG→CCA)	PP_4760 ←	alcohol dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,420,454	0	C	T	6.1%	300.8 / 5.6	98	P215P (CCG→CCA)	PP_4760	alcohol dehydrogenase
Reads supporting (aligned to +/- strand): ref base C (45/47); new base T (3/3); total (48/50)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.91e-01

Only 100 of 101 total aligned reads displayed.

CCCCGGTAAGGATGCCGATCAGCGAAACATGCCCGCCAATGCGCGCAGCAATCATCGACTGCTCCAGCGTCGCCGGGCCGCCCACTTCAATCACATGATCGACACCACGGTTGCCAGTCAGCTCGCGCACCTTCTCACCCCAGGCCGGGGTGCTCTTGTAGTTGATCAGGTGGTCGGCACCCAGGGCCTTCAGGCGCTCCAGCTTGGCATCGCTGGACGAGGTGGCGATCACGGTCGCGCCCGCCAGCTTGGCGAACTGCAGGGCGAAAATCGACACACCGCCGGTACCCTGC  >  NC_002947/5420309‑5420601

ccccGGTAAGGATGCCGATCAGCGAAACATGCCCGCCAATGCGCGCAGCAATCATCGACTGCTCCAGCGTCGCCGGGCCGCCCACTTCAATCACATGATCGACACCACGGTTGCCAGTCAGCTCGCGCACCTTCTCACCCCAGGCCggg                                                                                                                                                  <  4:107241/149‑1 (MQ=255)

  ccGGTAAGGATGCCGATCAGCGAAACATGCCCGCCAATGCGCGCAGCAATCATCGACTGCTCCAGCGTCGCCGGGCCGCCCACTTCAATCACATGATCGACACCACGGTTGCCAGTCAGCTCGCGCACCTTCTCACCCCAGGCCGGGGt                                                                                                                                                <  5:282601/149‑1 (MQ=255)

   cGGTAAGGATGCCGATCAGCGAAACATGCCCGCCAATGCGCGCAGCAATCATCGACTGCTCCAGCGTCGCCGGGCCGCCCACTTCAATCACATGATCGACACCACGGTTGCCAGTCAGCTCGCGCACCTTCTCACCCCGGCCTGGGGTg                                                                                                                                               <  6:213091/149‑1 (MQ=255)

    ggTAAGGATGCCGATCAGCGAAACATGCCCGCCAATGCGCGCAGCAATCATCGACTGCTCCAGCGTCGCCGGGCCGCCCACTTCAATCACATGATCGACACCACGGTTGCCAGTCAGCTCGCGCACCTTCTCACCCCGGCCTGGGGTga                                                                                                                                              <  1:374930/149‑2 (MQ=255)

      tAAGGATGCCGATCAGCGAAACATGCCCGCCAATGCGCGCAGCAATCATCGACTGCTCCAGCGTCGCCGGGCCGCCCACTTCAATCACATGATCGACACCACGGTTGCCAGTCAGCTCGCGCACCTTCTCACCCCAGGCCGGGGTGctc                                                                                                                                            <  6:344483/149‑1 (MQ=255)

         ggATGCCGATCAGCGAAACATGCCCGCCAATGCGCGCAGCAATCATCGACTGCTCCAGCGTCGCCGGGCCGCCCACTTCAATCACATGATCGACACCACGGTTGCCAGTCAGCTCGCGCACCTTCTCACCCCAGGCCGGGGTGCTCTTg                                                                                                                                         >  3:24845/1‑149 (MQ=255)

           aTGCCGATCAGCGAAACATGCCCGCCAATGCGCGCAGCAATCATCGACTGCTCCAGCGTCGCCGGGCCGCCCACTTCAATCACATGATCGACACCACGGTTGCCAGTCAGCTCGCGCACCTTCTCACCCCAGGCCGGGGTGCTCTTGTa                                                                                                                                       <  8:310625/149‑1 (MQ=255)

           aTGCCGATCAGCGAAACATGCCCGCCAATGCGCGCAGCAATCATCGACTGCTCCAGCGTCGCCGGGCCGCCCACTTCAATCACATGATCGACACCACGGTTGCCAGTCAGCTCGCGCACCTTCTCACCCCAGGCCGGGGTGCTCTTGTa                                                                                                                                       <  3:127553/149‑1 (MQ=255)

           aTGCCGATCAGCGAAACATGCCCGCCAATGCGCGCAGCAATCATCGACTGCTCCAGCGTCGCCGGGCCGCCCACTTCAATCACATGATCGACACCACGGTTGCCAGTCAGCTCGCGCACCTTCTCACCCCAGGCCGGGGTGCTCTTGTa                                                                                                                                       >  4:269002/1‑149 (MQ=255)

            tGCCGATCAGCGAAACATGCCCGCCAATGCGCGCAGCAATCATCGACTGCTCCAGCGTCGCCGGGCCGCCCACTTCAATCACATGATCGACACCACGGTTGCCAGTCAGCTCGCGCACCTTCTCACCCCAGGCCGGGGTGCTCTTGTAg                                                                                                                                      <  8:237227/149‑1 (MQ=255)

              ccGATCAGCGAAACATGCCCGCCAATGCGCGCAGCAATCATCGACTGCTCCAGCGTCGCCGGGCCGCCCACTTCAATCACATGATCGACACCACGGTTGCCAGTCAGCTCGCGCACCTTCTCACCCCAGGCCGGGGTGCTCTTGTAGtt                                                                                                                                    <  4:334502/149‑1 (MQ=255)

                gATCAGCGAAACATGCCCGCCAATGCGCGCAGCAATCATCGACTGCTCCAGCGTCGCCGGGCCGCCCACTTCAATCACATGATCGACACCACGGTTGCCAGTCAGCTCGCGCACCTTCTCACCCCAGGCCGGGGTGCTCTTGTAGTTGa                                                                                                                                  <  3:332149/149‑1 (MQ=255)

                 aTCAGCGAAACATGCCCGCCAATGCGCGCAGCAATCATCGACTGCTCCAGCGTCGCCGGGCCGCCCACTTCAATCACATGATCGACACCACGGTTGCCAGTCAGCTCGCGCACCTTCTCACCCCAGGCCGGGGTGCTCtt                                                                                                                                          <  7:206433/140‑1 (MQ=255)

                 aTCAGCGAAACATGCCCGCCAATGCGCGCAGCAATCATCGACTGCTCCAGCGTCGCCGGGCCGCCCACTTCAATCACATGATCGACACCACGGTTGCCAGTCAGCTCGCGCACCTTCTCACCCCAGGCCGGGGTGCTCtt                                                                                                                                          >  8:206433/1‑140 (MQ=255)

                  tCAGCGAAACATGCCCGCCAATGCGCGCAGCAATCATCGACTGCTCCAGCGTCGCCGGGCCGCCCACTTCAATCACATGATCGACACCACGGTTGCCAGTCAGCTCGCGCACCTTCTCACCCCAGGCCGGGGTGCTCTTGTAGTTGATc                                                                                                                                <  2:52619/149‑1 (MQ=255)

                    aGCGAAACATGCCCGCCAATGCGCGCAGCAATCATCGACTGCTCCAGCGTCGCCGGGCCGCCCACTTCAATCACATGATCGACACCACGGTTGCCAGTCAGCTCGCGCACCTTCTCACCCCAGGCCGGGGTGCTCTTGTAGTTGATCa                                                                                                                               <  7:165789/148‑1 (MQ=255)

                    aGCGAAACATGCCCGCCAATGCGCGCAGCAATCATCGACTGCTCCAGCGTCGCCGGGCCGCCCACTTCAATCACATGATCGACACCACGGTTGCCAGTCAGCTCGCGCACCTTCTCACCCCAGGCCGGGGTGCTCTTGTAGTTGATCa                                                                                                                               <  3:45607/148‑1 (MQ=255)

                    aGCGAAACATGCCCGCCAATGCGCGCAGCAATCATCGACTGCTCCAGCGTCGCCGGGCCGCCCACTTCAATCACATGATCGACACCACGGTTGCCAGTCAGCTCGCGCACCTTCTCACCCCAGGCCGGGGTGCTCTTGTAGTTGATCAg                                                                                                                              <  2:10434/149‑1 (MQ=255)

                            aTGCCCGCCAATGCGCGCAGCAATCATCGACTGCTCCAGCGTCGCCGGGCCGCCCACTTCAATCACATGATCGACACCACGGTTGCCAGTCAGCTCGCGCACCTTCTCACCCCAGGCCGGGGTGCTCTTGTAGTTGATCAGGTGGTCgg                                                                                                                      >  5:388273/1‑149 (MQ=255)

                             tGCCCGCCAATGCGCGCAGCAATCATCGACTGCTCCAGCGTCGCCGGGCCGCCCACTTCAATCACATGATCCACACCACGGTTGCCAGTCAGCTCGCGCACCTTCTCACCCCAGGCCGGGGTGCTCTTGTAGTTGATCAGGTGGTCGGc                                                                                                                     <  1:127850/149‑1 (MQ=255)

                                ccGCCAATGCGCGCAGCAATCATCGACTGCTCCAGCGTCGCCGGGCCGCCCACTTCAATCACATGATCGACACCACGGTTGCCAGTCAGCTCGCGCACCTTCTCACCCCAGGCCggg                                                                                                                                                  <  8:114665/117‑1 (MQ=255)

                                ccGCCAATGCGCGCAGCAATCATCGACTGCTCCAGCGTCGCCGGGCCGCCCACTTCAATCACATGATCGACACCACGGTTGCCAGTCAGCTCGCGCACCTTCTCACCCCAGGCCggg                                                                                                                                                  >  7:114665/1‑117 (MQ=255)

                                 cGCCAATGCGCGCAGCAATCATCGACTGCTCCAGCGTCGCCGGGCCGCCCACTTCAATCACATGATCGACACCACAGTTGCCAGTCAGCTCGCGCACCTTCTCACCCCAGGCCGGGGTGCTCTTGTAGTTGATCAGGTGGTCGGCAcc                                                                                                                  >  3:382797/1‑148 (MQ=255)

                                  gCCAATGCGCGCAGCAATCATCGACTGCTCCAGCGTCGCCGGGCCGCCCACTTCAATCACATGATCGACACCACGGTTGCCAGTCAGCTCGCGCACCTTCTCACCCCAGGCCGGGGTGCTCTTGTATTTGATCAGGTGGTCGGCACCCa                                                                                                                <  7:256734/149‑1 (MQ=255)

                                              agcaATCATCGACTGCTCCAGCGTCGCCGGGCCGCCCACTTCAATCACATGATCGACACCACGGTTGCCAGTCAGCTCGCGCACCTTCTCACCCCAGGCCggg                                                                                                                                                  >  8:304498/1‑103 (MQ=255)

                                              agcaATCATCGACTGCTCCAGCGTCGCCGGGCCGCCCACTTCAATCACATGATCGACACCACGGTTGCCAGTCAGCTCGCGCACCTTCTCACCCCAGGCCggg                                                                                                                                                  <  7:304498/103‑1 (MQ=255)

                                              agcaATCATCGACTGCTCCAGCGTCGCCGGGCCGCCCACTTCAATCACATGATCGACACCACGGTTGCCAGTCAGCTCGCGCACCTTCTCACCCCAGGCCGGGGTGCTCTTGTAGTTGATCAGGTGGTCGGCACCCAGGGCCTTCAGgc                                                                                                    <  1:332906/149‑1 (MQ=255)

                                                caATCATCGACTGCTCCAGCGTCGCCGGGCCGCCCACTTCAATCACATGATCGACACCACGGTTGCCAGTCAGCTCGCGCACCTTCTCACCCCAGGCCGGGGTGCTCTTGTAGTTGATCAGGTGGTCGGCACCCAGGGCCTTCAGgcgc                                                                                                  <  6:304087/149‑1 (MQ=255)

                                                      tcGACTGCTCCAGCGTCGCCGGGCCGCCCACTTCAATCACATGATCGACACCACGGTTGCCAGTCAGCTCGCGCACCTTCTCACCCCAGGCCGGGGTGCTCTTGTAGTTGATCAGGTGGTCGGCACCCAGGGCCTTCAGGCGCTCCAGc                                                                                            >  1:188672/1‑149 (MQ=255)

                                                         aCTGCTCCAGCGTCGCCGGGCCGCCCACTTCAATCACATGATCGACACCACGGTTGCCAGTCAGCTCGCGCACCTTCTCACCCCAGGCCGGGGTGCTCTTGTAGTTGATCAGGTGGTCGGCACCCAGGGCCTTCAGGCGCTCCAGCTTg                                                                                         <  5:74011/149‑1 (MQ=255)

                                                             cTCCAGCGTCGCCGGGCCGCCCACTTCAATCACATGATCGACACCACGGTTGCCAGTCAGCTCGCGCACCTTCTCACCCCAGGCCGGGGTGCTCTTGTAGTTGATCAGGTGGTCGGCACCCAGGGCCTTCAGGCGCTCCAGCTTGGCa                                                                                      <  4:402715/148‑1 (MQ=255)

                                                                cAGCGTCGCCGGGCCGCCCACTTCAATCACATGATCGACACCACGGTTGCCAGTCAGCTCGCGCACCTTCTCACCCCAGGCCGGGGTGCTCTTGTAGTTGATCAGGTGGTCGGCACCCAGGGCCTTCAGGCGCTCCAGCTTGGCATCGc                                                                                  <  8:194275/149‑1 (MQ=255)

                                                                 aGCGTCGCCGGGCCGCCCACTTCAATCACATGATCGACACCACGGTTGCCAGTCAGCTCGCGCACCTTCTCACCCCAGGCCGGGGTGCTCTTGTAGTTGATCAGGTGGTCGGCACCCAGGGCCTTCAGGCGCTCCAGCTTGGCATCGCt                                                                                 <  3:269002/149‑1 (MQ=255)

                                                                  gCGTCGCCGGGCCGCCCACTTCAATCACATGATCGACACCACGGTTGCCAGTCAGCTCGCGCACCTTCTCACCCCAGGCCGGGGTGCTCTTGTAGTTGATCAGGTGGTCGGCACCCAGGGCCTTCAGGCGCTCCAGCTTGGCATCGCt                                                                                 >  4:376943/1‑148 (MQ=255)

                                                                  gCGTCGCCGGGCCGCCCACTTCAATCACATGATCGACACCACGGTTGCCAGTCAGCTCGCGCACCTTCTCACCCCAGGCCGGGGTGCTCTTGTAGTTGATCAGGTGGTCGGCACCCAGGGCCTTCAGGCGCTCCAGCTTGGCATCGCt                                                                                 <  3:376943/148‑1 (MQ=255)

                                                                   cGTCGCCGGGCCGCCCACTTCAATCACATGATCGACACCACGGTTTCCAGTCAGCTCGCGCACCTTCTCACCCCAGGCCGGGGTGCTCTTGTAGTTGATCAGGTGGTCGGCACCCAGGGCCTTCAGGCGCTCCAGCTTGGCa                                                                                      >  1:322578/1‑142 (MQ=255)

                                                                   cGTCGCCGGGCCGCCCACTTCAATCACATGATCGACACCACGGTTACCAGTCAGCTCGCGCACCTTCTCACCCCAGGCCGGGGTGCTCTTGTAGTTGATCAGGTGGTCGGCACCCAGGGCCTTCAGGCGCTCCAGCTTGGCa                                                                                      <  2:322578/142‑1 (MQ=255)

                                                                    gTCGCCGGGCCGCCCACTTCAATCACATGATCGACACCACGGTTGCCAGTCAGCTCGCGCACCTTCTCACCCCAGGCCGGGGTGCTCTTGTAGTTGATCAGGTGGTCGGCACCCAGGGCCTTCAGGCGCTCCAGCTTGGCATCGCTGGa                                                                              >  5:137170/1‑149 (MQ=255)

                                                                    gTCGCCGGGCCGCCCACTTCAATCACATGATCGACACCACGGTTGCCAGTCAGCTCGCGCACCTTCTCACCCCAGGCCGGGGTGCCCTTGTAGTTGATCAGGTGGTCGGCACCCAGGGCCTTCAGGCGCTCCAGCTTGGCATCGCTGGa                                                                              >  5:228369/1‑149 (MQ=255)

                                                                    gTCGCCGGGCCGCCCACTTCAATCACATGATCGACACCACGGTTGCCAGTCAGCTCGAGCACCTTCTCACCCCAGGCCGGGGTGCTCTTGTAGTTGATCAGGTGGTCGGCACCCAGTGCCTTCAGGCGCTCCAGCTTGGAATCGCTGGa                                                                              >  2:106534/1‑149 (MQ=255)

                                                                       gCCGGGCCGCCCACTTCAATCACATGATCGACACCACGGTTGCCAGTCAGCTCGCGCACCTTCTCACCCCAGGCCGGGGTGCTCTTGTAGTTGATCAGGTGGTCGGCACCCAGGGCCTTCAGGCGCTCCAGCTTGGCATCGCTGGAc                                                                             >  5:26265/1‑147 (MQ=255)

                                                                         cGGGCCGCCCACTTCAATCACATGATCGACACCACGGTTGCCAGTCAGCTCGCGCACCTTCTCACCCCAGGCCGGGGTGCTCTTGTAGTTGATCAGGTGGTCGGCACCCAGGGCCTTCAGGCGCTCCAGCTTGGCATCGCTGGACGAgg                                                                         >  4:237335/1‑149 (MQ=255)

                                                                          gggCCGCCCACTTCAATCACATGATCGACACCACGGTTGCCAGTCAGCTCGCGCACCTTCTCACCCCAGGCCGGGGTGCTCTTGTAGTTGATCAGGTGGTCGGCACCCAGGGCCTTCAGGCGCTCCAGCTTGGCATCGCTGGACGAGGt                                                                        >  3:160314/1‑149 (MQ=255)

                                                                            gccgccCACTTCAATCACATGATCGACACCACAGTTGCCAGTCAGCTCGCGCACCTTCTCACCCCAGGCCGGGGTGCTCTTGTAGTTGATCAGGTGGTCGGCACCCAGGGCCTTCAGGCGCTCCAGCTTGGCATCGCTGGACGAGGTgg                                                                      <  4:382797/149‑1 (MQ=255)

                                                                              cgccCACTTCAATCACATGATCGACACCACGGTTGCCAGTCAGCTCGCGCACCTTCTCACCCCAGGCCGGGGTGCTCTTGTAGTTGATCAGGTGGTCGGCACCCAGGGCCTTCAGGCGCTCCAGCTTGGCATCGCTGGACGAGGTGGc                                                                     >  1:116814/1‑148 (MQ=255)

                                                                                   aCTTCAATCACATGATCGACACCACGGTTGCCAGTCAGCTCGCGCACCTTCTCACCCCAGGCCGGGGTGCTCTTGTAGTTGATCAGGTGGTCGGCACCCAGGGCCTTCAGGCGCTCCAGCTTGGCATCGCTGGACGAGGTGGCGATCAc                                                               >  4:239021/1‑149 (MQ=255)

                                                                                    cTTCAATCACATGATCGACACCACGGTTGCCAGTCAGCTCGCGCACCTTCTCACCCCAGGCCGGGGTGCTCTTGTAGTTGATCAGGTGGTCGGCACCCAGGGCCTTCAGGCGCTCCAGCTTGGCATCGCTGGACGAGGTGGCGATCACg                                                              <  6:137170/149‑1 (MQ=255)

                                                                                       cAATCACATGATCGACACCACGGTTGCCAGTCAGCTCGCGCACCTTCTCACCCCAGGCCGGGGTGCTCTTGTAGTTGAGCAGGTGGTCGGCACCCAGGGCCTTCAGGCGCTCCAGCTTGGCATCGCTGGACGAGGTGGCGATCACgg                                                             <  3:237335/147‑1 (MQ=255)

                                                                                       cAATCACATGATCGACACCACGGTTGCCAGTCAGCTCGCGCACCTTCTCACCCCAGGCCGGGGTGCTCTTGAAGTTGATCAGGTGGTCGGCACCCAGGGCCTTCAGGCGCTCCAGCTTGGCATCGCTGGACGAGGTGGCGATCACgg                                                             <  3:31689/147‑1 (MQ=255)

                                                                                        aaTCACATGATCGACACCACGGTTGCCAGTCAGCTCGCGCACCTTCTCACCCCAGGCCGGGGTGCTCTTGTAGTTGATCAGGTGGTCGGCACCCAGGGCCTTCAGGCGCTCCAGCTTg                                                                                         >  4:206665/1‑118 (MQ=255)

                                                                                        aaTCACATGATCGACACCACGGTTGCCAGTCAGCTCGCGCACCTTCTCACCCCAGGCCGGGGTGCTCTTGTAGTTGATCAGGTGGTCGGCACCCAGGGCCTTCAGGCGCTCCAGCTTGGCATCGCTGGACGAGGTGGCGATCACGGTc                                                           <  5:202461/148‑1 (MQ=255)

                                                                                         aTCACATGATCGACACCACGGTTGCCAGTCAGCTCGCGCACCTTCTCACCCCAGGCCGGGGTGCTCTTGTAGTTGATCAGGTGGTCGGCACCCAGGGCCTTCAGGCGCTCCAGCTTGGCATCGCTGGACGAGGTGGCGATCACGGTcgc                                                         >  5:23215/1‑149 (MQ=255)

                                                                                              aTGATCGACACCACGGTTGCCAGTCAGCTCGCGCACCTTCTCACCCCAGGCCGGGGTGCTCTTGTAGTTGATCAGGTGGTCGGCACCCAGGGCCTTCAGGCGCTCCAGCTTGGCATCGCTGGACGAGGTGGCGATCACGGTCGcgcccg                                                    >  3:113423/1‑149 (MQ=255)

                                                                                              aTGATCGACACCACGGTTGCCAGTCAGCTCGCGCACCTTCTCACCCCAGGCCGGGGTGCTCTTGTAGTTGATCAGGTGGTCGGCACCCAGGGCCTTCAGGCGCTCCAGCTTGGCATCGCTGGACGAGGTGGCGATCACGGTCGcgcccg                                                    >  3:266843/1‑149 (MQ=255)

                                                                                                  tCGACACCACGGTTGCCAGTCAGCTCGCGCACCTTCTCACCCCAGGCCGGGGTGCTCTTGTAGTTGATCAGGTGGTCGGCACCCAGGGCCTTCAGGCGCTCCAGCTTGGCATCGCTGGACGAGGTGGCGATCACGGTCGcgcccgc                                                   >  1:168416/1‑146 (MQ=255)

                                                                                                  tCGACACCACGGTTGCCAGTCAGCTCGCGCACCTTCTCACCCCAGGCCGGGGTGCTCTTGTAGTTGATCAGGTGGTCGGCACCCAGGGCCTTCAGGCGCTCCAGCTTGGCATCGCTGGACGAGGTGGCGATCACGGTCGcgcccgc                                                   <  2:168416/146‑1 (MQ=255)

                                                                                                  tCGACACCACGGTTGCCAGTCAGCTCGCGCACCTTCTCACCCCAGGCCGGGGTGCTCTTGTAGTTGATCAGGTGGTCGGCACCCAGGGCCTTCAGGCGCTCCAGCTTGGCATCGCTGGACGAGGTGGCGATCACGGTCGCGCCCGCCAg                                                >  2:270879/1‑149 (MQ=255)

                                                                                                      caccacGGTTGCCAGTCAGCTCGCGCACCTTCTCACCCCAGGCCGGGGTGCTCTTGTAGTTGATCAGGTGGTCGGCACCCAGGGCCTTCAGGCGCTCCAGCTTGGCATCGCTGGACGAGGTGGCGATCACGGTCGCGCCCGCCAGCTTg                                            >  7:142298/1‑149 (MQ=255)

                                                                                                      caccacGGTTGCCAGTCAGCTCGCGCACCTTCTCACCCCAGGCCGGGGTGCTCTTGTAGTTGATCAGGTGGTCGGCACCCAGGGCCTTCAGGCGCTCCAGCTTGGCATCGCTGGACGAGGTGGCGATCACGGTCGCGCCCGCCAGCTTg                                            <  3:137972/149‑1 (MQ=255)

                                                                                                       accacGGTTGCCAGTCAGCTCGCGCACCTTCTCACCCCAGGCCGGGGTGCTCTTGTAGTTGATCAGGTGGTCGGCACCCAGGGCCTTCAGGCGCTCCAGCTTGGCATCGCTGGACGAGGTGGCGATCACGGTCGCGCCCGCCAGCTTgg                                           >  1:6825/1‑149 (MQ=255)

                                                                                                         cacGGTTGCCAGTCAGCTCGCGCACCTTCTCACCCCAGGCCGGGGTGCTCTTGTAGTTGATCAGGTGGTCGGCACCCAGGGCCTTCAGGCGCTCCAGCTTGGCATCGCTGGACGAGGTGGCGATCACGGTCGCGCCCGCCAGCTTGGc                                          >  7:106005/1‑148 (MQ=255)

                                                                                                         cacGGTTGCCAGTCAGCTCGCGCACCTTCTCACCCCAGGCCGGGGTGCTCTTGTAGTTGATCAGGTGGTCGGCACCCAGGGCCTTCAGGCGCTCCAGCTTGGCATCGCTGGACGAGGTGGCGATCACGGTCGCGCCCGCCAGCTTGGc                                          >  4:136951/1‑148 (MQ=255)

                                                                                                         cacGGTTGCCAGTCAGCTCGCGCACCTTCTCACCCCAGGCCGGGGTGCTCTTGTAGTTGATCAGGTGGTCGGCACCCAGGGCCTTCAGGCGCTCCAGCTTGGCATCGCTGGACGAGGTGGCGATCACGGTCGCGCCCGCCAGCTTGGc                                          >  3:269369/1‑148 (MQ=255)

                                                                                                                gCCAGTCAGCTCGCGCACCTTCTCACCCCAGGCCGGGGTGCTCTTGTAGTTGATCAGGTGGTCGGCACCCAGGGCCTTCAGGCGCTCCAGCTTGGCATCGCTGGACGAGGTGGCGATCACGGTCGCGCCCGCCAGCTTGGCGAACTGc                                   <  4:266843/148‑1 (MQ=255)

                                                                                                                 ccAGTCAGCTCGCGCACCTTCTCACCCCAGGCCGGGGTGCTCTTGTAGTTGATCAGGTGGTCGGCACCCAGGGCCTTCAGGCGCTCCAGCTTGGCATCGCTGGACGAGGTGGCGATCACGGTCGCGCCCGCCAGCTTGGCGAACTGCAg                                 <  6:388273/149‑1 (MQ=255)

                                                                                                                  cAGTCAGCTCGCGCACCTTCTCACCCCAGGCCGGGGTGCTCTTGTAGTTGATCAGGTGGTCGGCACCCAGGGCCTTCAGGCGCTCCAGCTTGGCATCGCTGGACGAGGTGGCGATCACGGTCGCGCCCGCCAGCTTGGCGAACTGCAgg                                <  6:26265/149‑1 (MQ=255)

                                                                                                                  cAGTCAGCTCGCGCACCTTCTCACCCCAGGCCGGGGTGCTCTTGTAGTTGATCAGGTGGTCGGCACCCAGGGCCTTCAGGCGCTCCAGCTTGGCATCGCTGGACGAGGTGGCGATCACGGTCGCGCCCGCCAGCTTGGCGAACTGCAgg                                >  8:304104/1‑149 (MQ=255)

                                                                                                                   aGTCAGCTCGCGCACCTTCTCACCCCAGGCCGGGGTGCTCTTGTAGTTGATCAGGTGGTCGGCACCCAGGGCCTTCAGGCGCTCCAGCTTGGCATCGCTGGACGAGGTGGCGATCACGGTCGCGCCCGCCAGCTTGGCGAACTGCAggg                               <  3:239021/149‑1 (MQ=255)

                                                                                                                    gTCAGCTCGCGCACCTTCTCACCCCAGGCCGGGGTGCTCTTGTAGTTGATCAGGTGGTCGGCACCCAGGGCCtt                                                                                                         >  3:97537/1‑74 (MQ=255)

                                                                                                                    gTCAGCTCGCGCACCTTCTCACCCCAGGCCGGGGTGCTCTTGTAGTTGATCAGGTGGTCGGCACCCAGGGCCtt                                                                                                         <  4:97537/74‑1 (MQ=255)

                                                                                                                     tCAGCTCGCGCACCTTCTCACCCCAGGCCGGGGTGCTCTTGTAGTTGATCAGGTGGTCGGCACCCAGGGCCTTCAGGCGCTCCAGCTTGGCATCGCTGGACGAGGTGGCGATCACg                                                              >  7:284246/1‑116 (MQ=255)

                                                                                                                     tCAGCTCGCGCACCTTCTCACCCCAGGCCGGGGTGCTCTTGTAGTTGATCAGGTGGTCGGCACCCAGGGCCTTCAGGCGCTCCAGCTTGGCATCGCTGGACGAGGTGGCGATCACg                                                              <  8:284246/116‑1 (MQ=255)

                                                                                                                       aGCTCGCGCACCTTCTCACCCCAGGCCGGGGTGCTCTTGTAGTTGATCAGGTGGTCGGCACCCAGGGCCTTCAGGCGCTCCAGCTTGGCATCGCTGGACGAGGTGGCGATCACGGTCGCGCCCGCCAGCTTGGCGAACTGCAGGGCGa                            >  6:404058/1‑148 (MQ=255)

                                                                                                                         cTCGCGCACCTTCTCACCCCAGGCCGGGGTGCTCTTGTAGTTGATCAGGTGGTCGGCACCCAGGGCCTTCAGGCGCTCCAGCTTGGCATCGCTGGACGAGGTGGCGATCACGGTCGCGCCCGCCAGCTTGGCGAACTGCAGGGCGaaaa                         >  2:71020/1‑149 (MQ=255)

                                                                                                                            gcgcACCTTCTCACCCCAGGCCGGGGTGGTCTTGTAGTTGATCAGGTGGTCGGCACCCAGGGCCTTCAGGCGCTCCAGCTTGGCATCGCTGGACGAGGTGGCGATCACGGTCGCGCCCGCCAGCTTGGCGAACTGCAGGGCGAAAATc                       >  1:230929/1‑148 (MQ=255)

                                                                                                                            gcgcACCTTCTCACCCCAGGCCGGGGTGCTCTTGTAGTTGATCAGGTGGTCGGCACCCAGGGCCTTCAGGCGCTCCAGCTTGGCATCGCTGGACGAGGTGGCGATCACGGTCGCGCCCGCCAGCTTGGCGAACTGCAGGGCGAAAATc                       <  3:136951/148‑1 (MQ=255)

                                                                                                                            gcgcACCTTCTCACCCCAGGCCGGGGTGCTCTTGTAGTTGATCAGGTGGTCGGCACCCAGGGCCTTCAGGCGCTCCAGCTTGGCATCGCTGGACGAGGTGGCGATCACGGTCGCGCCCGCCAGCTTGGCGAACTGCAGGGCGAAAATCg                      <  2:116814/149‑1 (MQ=255)

                                                                                                                             cgcACCTTCTCACCCCAGGCCGGGGTGCTCTTGTAGTTGATCAGGTGGTCGGCACCCAGGGCCTTCAGGCGCTCCAGCTTGGCATCGCTGGACGAGGTGGCGATCACGGTCGCGCCCGCCAGCTTGGCGAACTGCAGGGCGAAAATCGa                     <  8:142298/149‑1 (MQ=255)

                                                                                                                             cgcACCTTCTCACCCCAGGCCGGGGTGCTCTTGTAGTTGATCAGGTGGTCGGCACCCAGGGCCTTCAGGCGCTCCAGCTTGGCATCGCTGGACGAGGTGGCGATCACGGTCGCGCCCGCCAGCTTGGCGAACTGCAGCGCGAAAATCGa                     >  8:29241/1‑149 (MQ=255)

                                                                                                                             cgcACCTTCTCACCCCAGGCCGGGGTGCCCTTGTAGTTGATCAGGTGGTCGGCACCCAGGGCCTTCAGGCGCTCCAGCTTGGCATCGCTGGACGAGGTGGCGATCACGGTCGCGCCCGCCAGCTTGGCGAACTGCAGGGCGAAAATCGa                     <  6:228369/149‑1 (MQ=255)

                                                                                                                              gcACCTTCTCACCCCAGGCCGGGGTGCTCTTGTAGTTGATCAGGTGGTCGGCACCCAGGGCCTTCAGGCGCTCCAGCTTGGCATCGCTGGACGAGGTGGCGATCACGGTCGCGCCCGCCAGCTTGGCGAACTGCAGGGCGAAAATCGa                     >  2:308064/1‑148 (MQ=255)

                                                                                                                              gcACCTTCTCACCCCAGGCCGGGGTGCTCTTGTAGTTGATCAGGTGGTCGGCACCCAGGGCCTTCAGGCGCTCCAGCTTGGCATCGCTGGACGAGGTGGCGATCACGGTCGCGCCCGCCAGCTTGGCGAACTGCAGGGCGAAAATCGa                     <  1:308064/148‑1 (MQ=255)

                                                                                                                                  cTTCTCACCCCAGGCCGGGGTGCTCTTGTAGTTGATCAGGTGGTCGGCACCCAGGGCCTTCAGGCGCTCCAGCTTGGCATCGCTGGACGAGGTGGCGATCACGGTCGCGCCCGCCAGCTTGGCGAACTGCAGGGCGAAAATCGacacac                >  2:72610/1‑149 (MQ=255)

                                                                                                                                    tctcACCCCAGGCCGGGGTGCTCTTGTAGTTGATCAGGTGGTCGGCACCCAGGGCCTTCAGGCGCTCCAGCTTGGCATCGCTGGACGAGGTGGCGATCACGGTCGCGCCCGCCAGCTTGGCGAACTGCAGGGCGAAAATCGACACAccg              >  1:269853/1‑149 (MQ=255)

                                                                                                                                    gagcACCCCGGCCTGGGGTGCTCTTGTAGTTGATCAGGTGGTCGGCACCCAGGGCCTTCAGGCGCTCCAGCTTGGCATCGCTGGACGAGGTGGCGATCACGGTCGCGCCCGCCAGCTTGGCGAACTGCAGGGCGAAAATCGa                     >  4:367787/4‑142 (MQ=255)

                                                                                                                                    gagcACCCCGGCCTGGGGTGCTCTTGTAGTTGATCAGGTGGTCGGCACCCAGGGCCTTCAGGCGCTCCAGCTTGGCATCGCTGGACGAGGTGGCGATCACGGTCGCGCCCGCCAGCTTGGCGAACTGCAGGGCGAAAATCGa                     <  3:367787/139‑1 (MQ=255)

                                                                                                                                    gagcACCCCGGCCTGGGGTGCTCTTGTAGTTGATCAGGTGGTCGGCACCCAGGGCCTTCAGGCGCTCCAGCTTGGCATCGCTGGACGAGGTGGCGATCACGGTCGCGCCCGCCAGCTTGGCGAACTGCAGGGCGAAAATCGACACAccg              >  2:105480/4‑149 (MQ=255)

                                                                                                                                    gagcACCCCGGCCTGGGGTGCTCTTGTAGTTGATCAGGTGGTCGGCACCCAGGGCCTTCAGGCGCTCCAGCTTGGCATCGCTGGACGAGGTGGCGATCACGGTCGCGCCCGCCAGCTTGGCGAACTGCAGGGCGAAAATCGACACAccg              >  3:296365/4‑149 (MQ=255)

                                                                                                                                      tcACCCCAGGCCGGGGTGCTCTTGTAGTTGATCAGGTGGTCGGCACCCAGGGCCTTCAGGCGCTCCAGCTTGGCATCGCTGGACGAGGTGGCGATCACGGTCGCGCCCGCCAGCTTGGCGAACTGCAGGGCGAAAATCGACACAccgcc            >  5:211552/1‑149 (MQ=255)

                                                                                                                                        aCCCCAGGCCGGGGTGCTCTTGTAGTTGATCAGGTGGTCGGCACCCAGGGCCTTCAGGCGCTCCAGCTTGGCATCGCTGGACGAGGTGGCGATCACGGTcgc                                                         <  6:336791/102‑1 (MQ=255)

                                                                                                                                        aCCCCAGGCCGGGGTGCTCTTGTAGTTGATCAGGTGGTCGGCACCCAGGGCCTTCAGGCGCTCCAGCTTGGCATCGCTGGACGAGGTGGCGATCACGGTcgc                                                         >  5:336791/1‑102 (MQ=255)

                                                                                                                                        aCCCCAGGCCGGGGTGCTCTTGTAGTTGATCAGGTGGTCGGCACCCAGGGCCTTCAGGCGCTCCAGCTTGGCATCGCTGGACGAGGTGGCGATCACGGTCGCGCCCGCCAGCTTGGCGAACTGCAGGGCGAAAATCGACACAccgcc            <  2:6825/147‑1 (MQ=255)

                                                                                                                                         ccccAGGCCGGGGTGCTCTTGTAGTTGATCAGGTGGTCGGCACCCAGGGCCTTCAGGCGCTCCAGCTTGGCATCGCTGGACGAGGTGGCGATCACGGTCGCGCCCGCCAGCTTGGCGAACTGCAGGGCGAAAATCGACACACCGCCGGt         <  4:113423/149‑1 (MQ=255)

                                                                                                                                          cccAGGCCGGGGTGCTCTTGTAGTTGATCAGGTGGTCGGCACCCAGGGCCTTCAGGCGCTCCAGCTTGGGATCGCTGGACGAGGTGGCGATCACGGTCGCGCCCGCCAGCTTGGCGAACTGCAGGGCGAAAATCGa                     <  3:136555/136‑1 (MQ=255)

                                                                                                                                          cccAGGCCGGGGTGCTCTTGTAGTTGATCAGGTGGTCGGCACCCAGGGCCTTCAGGCGCTCCAGCTTGGCATCGCTGGACGAGGTGGCGATCACGGTCGCGCCCGCCAGCTTGGCGAACTGCAGGGCGAAAATCGa                     >  4:136555/1‑136 (MQ=255)

                                                                                                                                           ccAGGCCGGGGTGCTCTTGTAGTTGATCAGGTGGTCGGCACCCAGGGCCTTCAGGCGCTCCAGCTTGGCATCGCTGGACGAGGTGGCGATCACGGTCGCGCCCGCCAGCTTGGCGAACTGCAGGGCGAATCTCGACCCAACGCCGGTAc       >  4:10670/1‑149 (MQ=255)

                                                                                                                                            cAGGCCGGGGTGCTCTTGTAGTTGATCAGGTGGTCGGCACCCAGGGCCTTCAGGCGCTCCAGCTTGGCATCGCTGGACGAGGTGGCGATCACGGTCGCGCCCGCCAGCTTGGCGAACTGCAGGGCGAAAATCGACACACCGCCGGTAcc      >  1:401929/1‑149 (MQ=255)

                                                                                                                                                ccGGGGTGCTCTTGTAGTTGATCAGGTGGTCGGCACCCAGGGCCTTCAGGCGCTCCAGCTTGGCATCGCTGGACGAGGTGGCGATCACGGTCGCGCCCGCCAGCTTGGCGAACTGCAGGGCGAAAATCGACACAccgccg           <  3:86856/140‑1 (MQ=255)

                                                                                                                                                ccGGGGTGCTCTTGTAGTTGATCAGGTGGTCGGCACCCAGGGCCTTCAGGCGCTCCAGCTTGGCATCGCTGGACGAGGTGGCGATCACGGTCGCGCCCGCCAGCTTGGCGAACTGCAGGGCGAAAATCGACACAccgccg           >  4:86856/1‑140 (MQ=255)

                                                                                                                                                ccGGGGTGCTCTTCTAGGTGATCAGGTGGTCGGCACCCAGGGCCTTCAGGCGCTCCAGCTTGGCATCGCTGGACGAGGTGGCGATCACGGTCGCGCCCGCCAGCTTGGCGAACTGCAGGGCGAAAATCGACACACCGCCGGTACCCTGc  <  4:24845/149‑1 (MQ=255)

CCCCGGTAAGGATGCCGATCAGCGAAACATGCCCGCCAATGCGCGCAGCAATCATCGACTGCTCCAGCGTCGCCGGGCCGCCCACTTCAATCACATGATCGACACCACGGTTGCCAGTCAGCTCGCGCACCTTCTCACCCCAGGCCGGGGTGCTCTTGTAGTTGATCAGGTGGTCGGCACCCAGGGCCTTCAGGCGCTCCAGCTTGGCATCGCTGGACGAGGTGGCGATCACGGTCGCGCCCGCCAGCTTGGCGAACTGCAGGGCGAAAATCGACACACCGCCGGTACCCTGC  >  NC_002947/5420309‑5420601

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`