Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,440,368	G→A	8.6%	A153V (GCG→GTG)	amn ←	AMP nucleosidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,440,368	0	G	A	8.6%	163.4 / 4.2	58	A153V (GCG→GTG)	amn	AMP nucleosidase
Reads supporting (aligned to +/- strand):  ref base G (31/22);  new base A (2/3);  total (33/25)
Fisher's exact test for biased strand distribution p-value = 6.43e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.06e-01

ACGGCTGTACATGGCGCCAGTCGCTGCCGGTGTAGTGCACCAGGCGGCGTAGCGAGAAGTCGACGCGCGCGGCATCGAACAACGCCAGTGGCATGATTTCAGCACGGTCCCAGTCGTACAGGCCATCAGCGATGTTGTCGGTGGCCGCCGACAGGTCGGTGCTGGGGAACACCCGCGCCAGCTGCGCAGCGGTAATGCCGCTACCGGCCAGCTCGTCGCCCTGGTCGACCACATAGGGGTAAGGGATATTCTGCTCGCTGACGCCGACTTCTACCGTAACGGTGTAGTCATGCA  >  NC_002947/5440222‑5440515                                                                                                                                                   |                                                                                                                                                    aCGGCTGTACATGGCGCCAGTCGCTGCCGGTGTAGTGCACCAGGCGGCGTAGCGAGAAGTCGACGCGCGCGGCATCGAACAACGCCAGTGGCATGATTTCAGCACGGTCCCAGTCGTACAGGCCATCAGCGATGTTGTCGGTGgccgcc                                                                                                                                                   >  2:193970/1‑149 (MQ=255)        tACATGGCGCCAGTCGCTGCCGGTGTAGTGCACCAGGCGGCGTAGCGAGAAGTCGACGCGCGCGGCATCGAACAACGCCAGTGGCATGATTTCAGCACGGTCCCAGTCGTACAGGCCATCAGCGATGTTGTCGGCGGCCACCGACaaca                                                                                                                                            <  1:404292/149‑4 (MQ=255)          cATGGCGCCAGTCGCTGCCGGTGTAGTGCACCAGGCGGCGTAGCGAGAAGTCGACGCGCGCGGCATCGAACAACGCCAGGGGCATGATTTCAGCACGGTCCCAGTCGTACAGGCCATCAGCGATGTTGTCGGCGGCCACCGACaacatc                                                                                                                                          <  4:16158/149‑6 (MQ=255)                gcCAGTCGCTGCCGGTGTAGTGCACCAGGCGGCGTAGCGAGAAGTCGACGCGCGCGGCATCGAACAACGCCAGTGGCATGATTTCAGCACGGTCCCAGTCGTACAGGCCATCAGCGATGTTGTCGGTGGCCGCCGACAGGTCGGTGCTg                                                                                                                                    >  4:128536/1‑149 (MQ=255)                 ccAGTCGCTGCCGGTGTAGTGCACCAGGCGGCGTAGCGAGAAGTCGACGCGCGCGGCATCGAACAACGCCAGTGGCATGATTTCAGCACGGTCCCAGTCGTACAGGCCATCAGCGATGTTGTCGGTGGCCGCCGACAGGTCGGTGCTgg                                                                                                                                   <  3:235001/149‑1 (MQ=255)                  cAGTCGCTGCCGGTGTAGTGCACCAGGCGGCGTAGCGAGAAGTCGACGCGCGCGGCATCGAACAACGCCAGTGGCATGATTTCAGCACGGTCCCAGTCGTACAGGCCATCAGCGATGTTGTCGGTGGCCGCCGACAGGTCGGTGCTggg                                                                                                                                  <  5:272096/149‑1 (MQ=255)                           ccGGTGTAGTGCACCAGGCGGCGTAGCGAGAAGTCGACGCGCGCGGCATCGAACAACGCCAGTGGCATGATTTCAGCACGGTCCCAGTCGTACAGGCCATCAGCGATGTTGTCGGTGGCCGCCGACAGGTCGGAGCTGGGGAACACCcg                                                                                                                         >  3:301220/1‑149 (MQ=255)                                   gTGCACCAGGCGGCGTAGCGAGAAGTCGACGCGCGCGGCATCGAACAACGCCAGTGGCATGATTTCAGCACGGTCCCAGTCGTACAGGCCATCAGCGATGTTGTCGGTGGCCGCCGACAGGTCGGTGCTGGGGAACACCCGCGCCAGCt                                                                                                                 >  8:307839/1‑149 (MQ=255)                                   gTGCACCAGGCGGCGTAGCGAGAAGTCGACGCGCGCGGCATCGAACAACGCCAGTGGCATGATTTCAGCACGGTCCCAGTCGTACAGGCCATCAGCGATGTTGTCGGTGGCCGCCGACAGGTCGGTGCTGGGGAACACCCGCGCCAGCt                                                                                                                 >  3:369893/1‑149 (MQ=255)                                    tGCACCAGGCGGCGTAGCGAGAAGTCGACGCGCGCGGCATCGAACAACGCCAGTGGCATGATTTCAGCACGGTCCCAGTCGTACAGGCCATCAGCGATGTTGTCGGTGGCCGCCGACAGGTCGGTGCTGGGGAACACCCGCGCCAGCTg                                                                                                                >  2:248383/1‑149 (MQ=255)                                        ccAGGCGGCGTAGCGAGAAGTCGACGCGCGCGGCATCGAACAACGCCAGTGGCATGATTTCAGCACGGTCCCAGTCGTACAGGCCATCAGCGATGTTGTCGGTGGCCGCCGACAGGTCGGTGCTGGGGAACACCCGCGCCAGCTgcgc                                                                                                             >  1:64754/1‑148 (MQ=255)                                        ccAGGCGGCGTAGCGAGAAGTCGACGCGCGCGGCATCGAACAACGCCAGTGGCATGATTTCAGCACGGTCCCAGTCGTACAGGCCATCAGCGATGTTGTCGGTGGCCGCCGACAGGTCGGTGCTGGGGAACACCCGCGCCAGCTgcgc                                                                                                             <  2:64754/148‑1 (MQ=255)                                            gcggcgTAGCGAGAAGTCGACGCGCGCGGCATCGAACAACGCCAGTGGCATGATTTCAGCACGGTCCCAGTCGTACAGGCCATCAGCGATGTTGTCGGTGGCCGCCGACAGGTCGGTGCTGGGGAACACCcgc                                                                                                                        <  5:138927/133‑1 (MQ=255)                                            gcggcgTAGCGAGAAGTCGACGCGCGCGGCATCGAACAACGCCAGTGGCATGATTTCAGCACGGTCCCAGTCGTACAGGCCATCAGCGATGTTGTCGGTGGCCGCCGACAGGTCGGTGCTGGGGAACACCcgc                                                                                                                        >  6:138927/1‑133 (MQ=255)                                                  tAGCGAGAAGTCGACGCGCGCGGCATCGAACAACGCCAGTGGCATGATTTCAGCACGGTCCCAGTCGTACAGGCCATCAGCGATGTTGTCGGTGGCCGCCGACAGGTCGGTGCTGGGGAACACCCGCGCCAGCTGCGCAGCGGTAATGc                                                                                                  >  3:104224/1‑149 (MQ=255)                                                      gagaAGTCGACGCGCGCGGCATCGAACAACGCCAGTGGCATGATTTCAGCACGGTCCCAGTCGTACAGGCCATCAGCGATGTTGTCGGTGGCCGCCGACAGGTCGGTGCTGGGGAACAcc                                                                                                                           >  7:46219/1‑120 (MQ=255)                                                      gagaAGTCGACGCGCGCGGCATCGAACAACGCCAGTGGCATGATTTCAGCACGGTCCCAGTCGTACAGGCCATCAGCGATGTTGTCGGTGGCCGCCGACAGGTCGGTGCTGGGGAACAcc                                                                                                                           <  8:46219/120‑1 (MQ=255)                                                          aGTCGACGCGCGCGGCATCGAACAACGCCAGTGGCATGATTTCAGCACGGTCCCAGTCGTACAGGCCATCAGCGATGTTGTCGGTGGCCGCCGACAGGTCGGTGCTGGGGAACACCCGCGCCAGCTGCGCAGCGGTAATGCCGCTACCg                                                                                          <  2:347197/149‑1 (MQ=255)                                                                   gcgcgGCATCGAACAACGCCAGTGGCATGATTTCAGCACGGTCCCAGTCGTACAGGCCATCAGCGATGTTGTCGGTGGCCGCCGACAGGTCGGTGCTGGGGAACACCCGCGCCAGCTGCGCAGCGGTAATGCCGCTACCGGCCAGCtcg                                                                                 >  4:143051/1‑149 (MQ=255)                                                                   gcgcgGCATCGAACAACGCCAGTGGCATGATTTCAGCACGGTCCCAGTCGTACAGGCCATCAGCGATGTTGTCGGTGGCCGCCGACAGGTCGGTGCTGGGGAACACCCGCGCCAGCTGCGCAGCGGTAATGCCGCTACCGGCCAGCtcg                                                                                 <  7:307839/149‑1 (MQ=255)                                                                              aacaacGCCAGTGGCATGATTTCAGCACGGTCCCAGTCGTACAGGCCATCAGCGATGTTGTCGGTGGCCGCCGACAGGTCGGTGCTGGGGAACACCCGCGCCAGCTGCGCAGCGGTAATGCCGCTACCGGCCAGCTCGTCGCCCTGGTc                                                                      >  7:36797/1‑149 (MQ=255)                                                                                   cGCCAGTGGCATGATTTCAGCACGGTCCCAGTCGTACAGGCCATCAGCGATGTTGTCGGTGGCCGCCGACAGGTCGGTGCTGGGGAACACCCGCGCCAGCTGCGCAGCGGTAATGCCGCTACCGGCCAGCTCGTCGCCCTGGTCGGCca                                                                 <  3:143051/149‑1 (MQ=255)                                                                                     ccAGTGGCATGATTTCAGCACGGTCCCAGTCGTACAGGCCATCAGCGATGTTGTCGGTGGCCGCCGACAGGTCGGTGCTGGGGAACACCCGCGCCAGCTGCGCAGCGGTAATGCCGCTACCGGCCAGCTCGTCGCCCTGGTCGAcc                                                                  >  2:52700/1‑146 (MQ=255)                                                                                     ccAGTGGCATGATTTCAGCACGGTCCCAGTCGTACAGGCCATCAGCGATGTTGTCGGTGGCCGCCGACAGGTCGGTGCTGGGGAACACCCGCGCCAGCTGCGCAGCGGTAATGCCGCTACCGGCCAGCTCGTCGCCCTGGTCGAcc                                                                  <  1:52700/146‑1 (MQ=255)                                                                                       aGTGGCATGATTTCAGCACGGTCCCAGTCGTACAGGCCATCAGCGATGTTGTCGGTGGCCGCCGACAGGTCGGTGCTGGGGAACACCCGCGCCAGCTGCGCAGCGGTAATGCCGCTACCGGCCAGCTCGTCGACCAGGGCGAcgagctg                                                             <  1:193970/149‑7 (MQ=255)                                                                                            cATGATTTCAGCACGGTCCCAGTCGTACAGGCCATCAGCGATGTTGTCGGTGGCCGCCGACAGGTCGGTGCTGGGGAACACCCGCGCCAGCTGCGCAGCGGTAATGCCGCTACCGGCCAGCTCGTCGCCCTGGTCGACCACATAgggg                                                         >  6:386015/1‑148 (MQ=255)                                                                                             aTGATTTCAGCACGGTCCCAGTCGTACAGGCCATCAGCGATGTTGTCGGTGGCCGCCGACAGGTCGGTGCTGGGGAACACCCGCGCCAGCTGCGCAGCGGTAATGCCGCTACCGGCCAGCTCGTCGCCCTGGTCGACCACATAGGGGt                                                        >  4:326477/1‑148 (MQ=255)                                                                                              tGATTTCAGCACGGTCCCAGTCGTACAGGCCATCAGCGATGTTGTCGGTGGCCGCCGACAGGTCGGTGCTGGGGAACACCCGCGCCAGCTGCGCAGCGGTAATGCCGCTACCGGCCAGCTCGTCGCCCTGGTCGACCACATAGGGGt                                                        >  6:284490/1‑147 (MQ=255)                                                                                               gATTTCAGCACGGTCCCAGTCGTACAGGCCATCAGCGATGTTGTCGGTGGCCGCCGACAGGTCGGTGCTGGGGAACACCCGCGCCAGCTGCGCAGCGGTAATGCCGCTACCGGCCAGCTCGACGCCCTGGTCGACCACATAGACGTAAg                                                     >  8:278089/1‑149 (MQ=255)                                                                                                      gCACGGTCCCAGTCGTACAGGCCATCAGCGATGTTGTCGGTGGCCGCCGACAGGTCGGTGCTGGGGAACACCCGCGCCAGCTGCGCAGCGGTAATGCCGCTACCGGCCAGCTCGTCGCCCTGGTCGACCACATAGGGGTAAGGGATAtt                                              <  3:166074/149‑1 (MQ=255)                                                                                                       cACGGTCCCAGTCGTACAGGCCATCAGCGATGTTGTCGGTGGCCGGCGACAGGTCGGTGCTGGGGAACACCCGCGCCAGCTGCGCAGCGGTAATGCCGCTACCGGCCAGCTCGTCGCCCTGGTCGACCACATAGGGGTAAGGGATATTc                                             >  1:285698/1‑149 (MQ=255)                                                                                                        aCGGTCCCAGTCGTACAGGCCATCAGCGATGTTGTCGGTGGCCGCCGACAGGTCGGTGCTGGGGAACACCCGCGCCAGCTGCGCAGCGGTAATGCCGCTACCGGCCAGCTCGTCGCCCTGGTCGACCACATAGGGGTAAGGGATATTCt                                            <  1:248383/149‑1 (MQ=255)                                                                                                            tCCCAGTCGTACAGGCCATCAGCGATGTTGTCGGTGGCCGCCGACAGGTCGGTGCTGGGGAACACCCGCGCCAGCTGCGCAGCGGTAATGCCGCTACCGGCCAGCTCGTCGCCCTGGTCGACCACATAGGGGTAAGGGATATTCTGccc                                        >  5:165357/1‑147 (MQ=255)                                                                                                            tCCCAGTCGTACAGGCCATCAGCGATGTTGTCGGTGGCCGCCGACAGGTCGGTGCTGGGGAACACCCGCGCCAGCTGCGCAGCGGTAATGCCGCTACCGGCCAGCTCGTCGCCCTGGTCGACCACATAGGGGTAAGGGATATTCTGCTc                                        >  7:158177/1‑149 (MQ=255)                                                                                                             cccAGTCGTACAGGCCATCAGCGATGTTGTCGGTGGCCGCCGACAGGTCGGTGCTGGGGAACACCCGCGCCAGCTGCGCAGCGGTAATGCCGCTACCGGCCAGCTCGTCGCCCTGGTCGACCACATAGGGGTAAGGGATATTCTGCTCg                                       >  5:418236/1‑149 (MQ=255)                                                                                                              ccAGTCGTACAGGCCATCAGCGATGTTGTCGGTGGCCGCCGACAGGTCGGTGCTGGGGAACACCCGCGCCAGCTGCGCAGCGGTAATGCCGCTACCGGCCAGCTCGTCGCCCTGGTCGACca                                                                 <  5:263931/122‑1 (MQ=255)                                                                                                              ccAGTCGTACAGGCCATCAGCGATGTTGTCGGTGGCCGCCGACAGGTCGGTGCTGGGGAACACCCGCGCCAGCTGCGCAGCGGTAATGCCGCTACCGGCCAGCTCGTCGCCCTGGTCGACca                                                                 >  6:263931/1‑122 (MQ=255)                                                                                                              ccAGTCGTACAGGCCATCAGCGATGTTGTCGGTGGCCGCCGACAGGTCGGTGCTGGGGAACACCCGCGCCAGCTGCGCAGCGGTAATGCCGCTACCGGCCAGCTCGTCGCCCTGGTCGACCACATAGGGGTAAGGGATATTCTGCTCGc                                      >  4:212538/1‑149 (MQ=255)                                                                                                                     tACAGGCCATCAGCGATGTTGTCGGTGGCCGCCGACAGGTCGGTGCTGGGGAACACCCGCGCCAGCTGCGCAGCGGTAATGCCGCTACCGGCCAGCTCGTCGCCCTGGTCGACCACATAGGGGTAAGGGATATTCTGCTCGCTGACGcc                               >  1:270999/1‑149 (MQ=255)                                                                                                                       cAGGCCATCAGCGATGTTGTCGGTGGCCGCCGACAGGTCGGTGCTGGGGAACACCCGCGCCAGCTGCGCAGCGGTAATGCCGCTACCGGCCAGCTCGTCGCCCTGGTCGACCACATAGGGGTAAGGGATATTCTGCTCGCTGACGCCGa                             <  3:99887/149‑1 (MQ=255)                                                                                                                          gCCATCAGCGATGTTGTCGGTGGCCGCCGACAGGTCGGTGCTGGGGAACACCCGCGCCAGCTGCGCAGCGGTAATGCCGCTACCGGCCAGCTCGTCGCCCTGGTCGACCACATAGGGGTAAGGGATATTCTGCTCGCTGACGCCGACtt                          >  5:205154/1‑149 (MQ=255)                                                                                                                            cATCAGCGATGTTGTCGGTGGCCGCCGACAGGTCGGTGCTGGGGAACACCCGCGCCAGCTGCGCAGCGGTAATGCCGCTACCGGCCAGCTCGTCGCCCTGGTCGACCACATAGGGGTAAGGGATATTCTGCTCGCTGACGCCGACTTCt                        <  8:158177/149‑1 (MQ=255)                                                                                                                             aTCAGCGATGTTGTCGGTGGCCGCCGACAGGTCGGTGCTGGGGAACACCCGCGCCAGCTGCGCAGCGGTAATGCCGCTACCGGCCAGCTCGTCGCCCTGGTCGACCACATAGGGGTAAGGGATATTCTGCTc                                        >  6:227829/1‑132 (MQ=255)                                                                                                                             aTCAGCGATGTTGTCGGTGGCCGCCGACAGGTCGGTGCTGGGGAACACCCGCGCCAGCTGCGCAGCGGTAATGCCGCTACCGGCCAGCTCGTCGCCCTGGTCGACCACATAGGGGTAAGGGATATTCTGCTc                                        <  5:227829/132‑1 (MQ=255)                                                                                                                              tCAGCGATGTTGTCGGTGGCCGCCGACAGGTCGGTGCTCGGGAACACCCGCGCCAGCTGCGCAGCGGTAATGCCGCAACCGGCCAGCTCGTCGCCCTGGTCGACCACATAGGGGTAAGGGATATTCTGCTCGCTGACGCCGACTTCTAc                      >  8:127693/1‑149 (MQ=255)                                                                                                                                aGCGATGTTGTCGGTGGCCGCCGACAGGTCGGTGCTGGGGAACACCCGCGCCAGCTGCGCAGCGGTAATGCCGCTACCGGCCAGCTCGTCGCCCTGGTCGACCACATAGGGGTAAGGGATATTCTGCTCGCTGACGCCGACTTCTAcc                     <  3:326477/148‑1 (MQ=255)                                                                                                                                aGCGATGTTGTCGGTGGCCGCCGACAGGTCGGTGCTGGGGAACACCCGCGCCAGCTGCGCAGCGGTAATGCCGCTACCGGCCAGCTCGTCGCCCTGGTCGACCACATAGGGGTAAGGGATATTCTGCTCGCTGACGCCGACTTCTAcc                     <  3:212538/148‑1 (MQ=255)                                                                                                                                   gATGTTGTCGGTGGCCGCCGACAGGTCGGTGCTGGGGAACACCCGCGCCAGCTGCGCAGCGGTAATGCCGCTACCGGCCAGCTCGTCGCCCTGGTCGACCACATAGGGGTAAGGGATATTCTGCTCGCTGACGCCGACTTCTACCGTaa                 <  3:128536/149‑1 (MQ=255)                                                                                                                                   cgacctgtCGGCGGCCACCGACAGGTCGGTGCTGGGGAACACCCGCGCCAGCTGCGCAGCGGTAATGCCGCTACCGGCCAGCTCGTCGCCCTGGTCGACCACATAGGGGTAAGGGATATTCTGCTCGCTGACGCCGACTTCTAc                      >  5:358013/6‑144 (MQ=255)                                                                                                                                   cgacctgtCGGCGGCCACCGACAGGTCGGTGCTGGGGAACACCCGCGCCAGCTGCGCAGCGGTAATGCCGCTACCGGCCAGCTCGTCGCCCTGGTCGACCACATAGGGGTAAGGGATATTCTGCTCGCTGACGCCGACTTCTAc                      <  6:358013/139‑1 (MQ=255)                                                                                                                                     tgttgtCGGTGGCCGCCGACAGGTCGGTGCTGGGGAACACCCGCGCCAGCTGCGCAGCGGTAATGCCGCTACCGGCCAGCTCGTCGCCCTGGTCGACCACATAGGGGTAAGGGATATTCTGCTCGCTGACGCCGACTTCtaccgtaccg               >  1:385983/1‑149 (MQ=255)                                                                                                                                     tgttgtCGGTGGCCGCCGACAGGTCGGTGCTGGGGAACACCCGCGCCAGCTGCGCAGCGGTAATGCCGCTACCGGCCAGCTCGTCGCCCTGGTCGACCACATAGGGGTAAGGGATATTCTGCTCGCTGACGCCGACTTCTACCGTAACg               <  2:270999/149‑1 (MQ=255)                                                                                                                                     acctgtCGGCGGCCACCGACAGGTCGGTGCTGGGGAACACCCGCGCCAGCTGCGCAGCGGTAATGCCGCTACCGGCCAGCTCGTCGCCCTGGTCGACCACATAGGGGTAAGGGATATTCTGCTCGCTGACGCCGACTTCTACCGTAACg               >  2:3518/4‑149 (MQ=255)                                                                                                                                          tCGGTGGCCGCCGACAGGTCGGTGCTGGGGAACACCCGCGCCAGCTGCGCAGCGGTAATGCCGCTACCGGCCAGCTCGTCGCCCTGGTCGACCACATAggg                                                          >  1:254665/1‑101 (MQ=255)                                                                                                                                          tCGGTGGCCGCCGACAGGTCGGTGCTGGGGAACACCCGCGCCAGCTGCGCAGCGGTAATGCCGCTACCGGCCAGCTCGTCGCCCTGGTCGACCACATAggg                                                          <  2:254665/101‑1 (MQ=255)                                                                                                                                          tCGGTGGCCGCCGACAGGTCGGTGCTGGGGAACACCCGCGCCAGCTGCGCAGCGGTAATGCCGCTACCGGCCAGCTCGTCGCCCTGGTCGACCACATAGGGGTAAGGGATATTCTGCTCGCTGACGCCGACTTCTACCGTAACGgtg            >  7:297319/1‑147 (MQ=255)                                                                                                                                             gTGGCCGCCGACAGGTCGGTGCTGGGGAACACCCGCGCCAGCTGCGCAGCGGTACTGCCGCTACCGGCCGGCTCGTCGCCGTGGTCGACCAAATAGGGGTAAGGGATATTCTGCTCGCTGACGCCGACTTCTTCCGTAACGGTGTAGt        >  5:4033/1‑148 (MQ=255)                                                                                                                                             gTGGCCGCCGACAGGTCGGTGCTGGGGAACACCCGCGCCAGCTGCGCAGCGGTAATGCCGCTACCGGCCAGCTCGTCGCCCTGGTCGACCACATAGGGGTAAGGGATATTCTGCTCGCTGACGc                                <  4:253193/124‑1 (MQ=255)                                                                                                                                             gTGGCCGCCGACAGGTCGGTGCTGGGGAACACCCGCGCCAGCTGCGCAGCGGTAATGCCGCTACCGGCCAGCTCGTCGCCCTGGTCGACCACATAGGGGTAAGGGATATTCTGCTCGCTGACGc                                >  3:253193/1‑124 (MQ=255)                                                                                                                                                  cgccgACAGGTCGGTGCTGGGGAACACCCGCGCCAGCTGCGCAGCGGTAATGCCGCTACCGGCCAGCTCGTCGCCCTGGTCGACCACATAGGGGTAAGGGATATTCTGCTCGCTGACGCCGACTTCTACCGTAACGGTGTAGTCATGCa  >  2:256307/1‑149 (MQ=255)                                                                                                                                                   |                                                                                                                                                    ACGGCTGTACATGGCGCCAGTCGCTGCCGGTGTAGTGCACCAGGCGGCGTAGCGAGAAGTCGACGCGCGCGGCATCGAACAACGCCAGTGGCATGATTTCAGCACGGTCCCAGTCGTACAGGCCATCAGCGATGTTGTCGGTGGCCGCCGACAGGTCGGTGCTGGGGAACACCCGCGCCAGCTGCGCAGCGGTAATGCCGCTACCGGCCAGCTCGTCGCCCTGGTCGACCACATAGGGGTAAGGGATATTCTGCTCGCTGACGCCGACTTCTACCGTAACGGTGTAGTCATGCA  >  NC_002947/5440222‑5440515

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑