Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 79,895 C→A 5.5% P217Q (CCG→CAG)  PP_0069 → rossmann fold nucleotide‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00294779,8950CA 5.5% 233.1 / 3.0 72P217Q (CCG→CAG) PP_0069rossmann fold nucleotide‑binding protein
Reads supporting (aligned to +/- strand):  ref base C (27/41);  new base A (2/2);  total (29/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.21e-01

GGGCCGCGTTGCAGGCTGGCGGCGGCACGATTGCCGTGCTCGGTACGGGCTTGCAAAAACTTTATCCACAGCGCCATCGGGACCTTGCGCAGGCGATGATCGACAACGGCAGTG‑CGCTGGTTTCCGAGTATCCCCTGGATGCCGGGCCGCTGCCTGGCAACTTCCCCCGGCGCAACCGCATCATCAGTGGGCTGTCGCTCGGTGTGCTGGTGGTCGAGGCAAGCCTCGCCAGCGGCTCGCTGATCACGGCGCGTCTGGCGGCGGAACAAGGCCGCGAGGTGTACGCCATTC  >  NC_002947/79748‑80038
                                                                                                                                                    |                                                                                                                                               
gggCCGCGTTGCAGGCTGGCGGCGGCACGATTGCCGTGCTCGGTACGGGCTTGCAAAAACTTTATCCACAGCGCCATCGGGACCTTGCGCAGGCGATGATCGACAACGGCAGTG‑CGCTGGTTTCCGAGTATCCCCTGGATGCCGGGCCg                                                                                                                                                >  4:263718/1‑149 (MQ=255)
 ggCCGCGTTGCAGGCTGGCGGCGGCACGATTGCCGTGCTCGGTACGGGCTTGCAAAAACTTTATCCACAGCGCCATCGGGACCTTGCGCAGGCGATGATCGACAACGGCAGTG‑CGCTGGTTTCCGAGTATCCCCTGGATGCCAGGCAGc                                                                                                                                               >  7:275066/1‑149 (MQ=255)
    cgcgTTGCAGGCTGGCGGCGGCACGATTGCCGTGCTCGGTACGGGCTTGCAAAAACTTTATCCACAGCGCCATCGGGACCTTGCGCAGGCGATGATCGACAACGGCAGTG‑CGCTGGTTTCCGAGTATCCCCTGGATGCCAGGCAGCGGc                                                                                                                                            >  3:316869/1‑149 (MQ=255)
      ggTTGCAGGCTGGCGGATGCACGATTGCCGTGCTCGGTACGGGATTGCAAAAACTTTATCCACAGCGCCATCGGGACGTTTCGCAGGATATTATCGACAAAGGCAGTT‑CTCTGGTTTCCGAGTATCCCGTGGATGCCGGGCCGCTTCCt                                                                                                                                          <  2:232194/148‑1 (MQ=255)
      cgTTGCAGGCTGGCGGCGGCACGATTGCCGTGCTCGGTACGGGCTTGCAAAAACTTTATCCACAGCGCCATCGGGACCTTGCGCAGGCGATGATCGACAACGGCAGTG‑CGCTGGTTTCCGAGTATCCCCTGGATGCCGGGCCGCTGCCt                                                                                                                                          <  2:3048/149‑1 (MQ=255)
           cAGGCTGGCGGCGGCACGATTGCCGTGCTCGGTACGGGCTTGCAAAAACTTTATCCACAGCGCCATCGGGACCTTGCGCAGGCGATGATCGACAACGGCAGTG‑CGCTGGTTTCCGAGTATCCCCTGGATGCCGGGCCGCTGCCTGGCaa                                                                                                                                     <  3:87435/149‑1 (MQ=255)
            aGGCTGGCGGCGGCACGATTGCCGTGCTCGGTACGGGCTTGCAAAAACTTTATCCACAGCGCCATCGGGACCTTGCGCAGGCGATGATCGACAACGGCAGTG‑CGCTGGTTTCCGAGTATCCCCTGGATGCCGGGCCGCTGCCTGGCAAc                                                                                                                                    >  4:61788/1‑149 (MQ=255)
             ggCTGGCGGCGGCACGATTGCCGTGCTCGGTACGGGCTTGCAAAAACTTTATCCACAGCGCCATCGGGACCTTGCGCAGGCGATGATCGACAACGGCAGTG‑CGCTGGTTTCCGAGTATCCCCTGGATGCCAGGCAGCGGCCCGGCatcc                                                                                                                                   <  8:275066/149‑4 (MQ=255)
              gCTGGCGGCGGCACGATTGCCGTGCTCGGTACGGGCTTGCAAAAACTTTATCCACAGCGCCATCGGGACCTTGCGCAGGCGATGATCGACAACGGCAGTG‑CGCTGGTTTCCGAGTATCCCCTGGATGCCGGGCCGCTGCCTGGCAACtt                                                                                                                                  >  3:226584/1‑149 (MQ=255)
              gCTGGCGGCGGCACGATTGCCGTGCTCGGTACGGGCTTGCAAAAACTTTATCCACAGCGCCATCGGGACCTTGCGCAGGCGATGATCGACAACGGCAGTG‑CGCTGGTTTCCGAGTATCCCCTGGATGCCAGGCAGCGGCCCGGCatcca                                                                                                                                  <  4:316869/149‑5 (MQ=255)
                      cggcACGATTGCCGTGCTCGGTACGGGCTTGCAAAAACTTTATCCACAGCGCCATCGGGACCTTGCGCAGGCGATGATCGACAACGGCAGTG‑CGCTGGTTTCCGAGTATCCCCTGGATGCCGGGCCGCTGCCTGGCAACTTCCCCCGgc                                                                                                                          <  4:226584/149‑1 (MQ=255)
                      cggcACGATTGCCGTGCTCGGTACGGGCTTGCAAAAACTTTATCCACAGCGCCATCGGGACCTTGCGCAGGCGATGATCGACAACGGCAGTG‑CGCTGGTTTCCGAGTATCCCCTGGATGCCGGGCCGCTGCCTGGCAACTTCCCCCGgc                                                                                                                          <  1:37856/149‑1 (MQ=255)
                      cggcACGATTGCCGTGCTCGGTACGGGCTTGCAAAAACTTTATCCACAGCGCCATCGGGACCTTGCGCAGGCGATGATCGACAACGGCAGTG‑CGCTGGTTTCCGAGTATCCCCTGGATGCCGGGCCGCTGCCTGGCAACTTCCCCCGgc                                                                                                                          <  3:250753/149‑1 (MQ=255)
                      cggcACGATTGCCGTGCTCGGTACGGGCTTGCAAAAACTTTATCCACAGCGCCATCGGGACCTTGCGCAGGCGATGATCGACAACGGCAGTG‑CGCTGGTTTCCGAGTATCCCCTGGATGCCGGGCCGCTGCCTGGCAACTTCCCCCGgc                                                                                                                          <  6:175952/149‑1 (MQ=255)
                       ggcACGATTGCCGTGCTCGGTACGGGCTTGCAAAAACTTTATCCACAGCGCCATCGGGACCTTGCGCAGGCGATGATCGACAACGGCAGTG‑CGCTGGTTTCCGAGTATCCCCTGGATGCCGGGCCGCTGCCTGGCAACTTCCCCCGgcg                                                                                                                         >  3:180192/1‑149 (MQ=255)
                         cACGATTGCCGTGCTCGGTACGGGCTTGCAAAAACTTTATCCACAGCGCCATCGGGACCTTGCGCAGGCGATGATCGACAACGGCAGTG‑CGCTGGTTTCCGAGTATCCCCTGGATGCCGGGCCGCTGCCTGGCAACTTCCCCCGgcgc                                                                                                                        >  8:222018/1‑148 (MQ=255)
                                gCCGTGCTCGGTACGGGCTTGCAAAAACTTTATCCACAGCGCCATAGGGACCTAGCGCAGGCGATGATCGACAAAGGCAGTG‑CGCTGGTTTCCGAGTATCCACAGGATGACGGGCCGCTGCCTGGCAACTTCACCCGGCGCAACCgga                                                                                                                 >  7:385287/1‑146 (MQ=255)
                                 ccGTGCTCGGTACGGGCTTGCAAAAACTTTATCCACAGCGCCATCGGGACCTTGCGCAGGCGATGATCGACAACGGCAGTG‑CGCTGGTTTCCGAGTATCCCCTGGATGCCGGGCCGCTGCCTGGCAACTTCCCCCGGCGCAACCGcatc                                                                                                               <  2:103890/149‑1 (MQ=255)
                                   gTGCTCGGTACGGGCTTGCAAAAACTTTATCCACAGCGCCATCGGGACCTTGCGCAGGCGATGATCGACAACGGCAGTG‑CGCTGGTTTCCGAGTATCCCCTGGATGCCGGGCCGCTGCCTGGCAACTTCCCCCGGCGCAACCGcatcat                                                                                                             <  7:222018/149‑1 (MQ=255)
                                    tGCTCGGTACGGGCTTGCAAAAACTTTATCCACAGCGCCATCGGGACCTTGCGCAGGCGATGATCGACAACGGCAGTG‑CGCTGGTTTCCGAGTATCCCCTGGATGCCGGGCCGCTGCCTGGCAACTTCCCCCGGCGCAACCGCatcatc                                                                                                            <  3:61349/149‑1 (MQ=255)
                                         ggTACGGGCTTGCAAAAACTTTATCCACAGCGCCATCGGGACCTTGCGCAGGCGATGATCGACAACGGCAGTG‑CGCTGGTTTCCGAGTATCCCCTGGATGCCGGGCCGCTGCCTGGCAACTTCCCCCGGCGCAACCGCATCATCAGTgg                                                                                                       <  4:196867/149‑1 (MQ=255)
                                         ggTACGGGCTTGCAAAAACTTTATCCACAGCGCCATCGGGACCTTGCGCAGGCGATGATCGACAACGGCAGTG‑CGCTGGTTTCCGAGTATCCCCTGGATGCCGGGCCGCTGCCTGGCAACTTCCCCCGGCGCAACCGCATCATCAGTg                                                                                                        <  5:418313/148‑1 (MQ=255)
                                         ggTACGGGCTTGCAAAAACTTTATCCACAGCGCCATCGGGACCTTGCGCAGGCGATGATCGACAACGGCAGTG‑CGCTGGTTTCCGAGTATCCCCTGGATGCCGGGCCGCTGCCTGGCAACTTCCCCCGGCGCAACCGCATCATCAGTg                                                                                                        <  3:261442/148‑1 (MQ=255)
                                          gTACGGGCTTGCAAAAACTTTATCCACAGCGCCATCGGGACCTTGCGCAGGCGATGATCGACAACGGCAGTG‑CGCTGGTTTCCGAGTATCCCCTGGATGCCGGGCCGCTGCCTGGCAACTTCCCCCGGCGCAACCGCATCATCAGTggg                                                                                                      <  6:9401/149‑1 (MQ=255)
                                          gTACGGGCTTGCAAAAACTTTATCCACAGCGCCATCGGGACCTTGCGCAGGCGATGATCGACAACGGCAGTG‑CGCTGGTTTCCGAGTATCCCCTGGATGCCGGGCCGCTGCCTGGCAACTTCCCCCGGCGCAACCGCATCATCAGTggg                                                                                                      <  3:263718/149‑1 (MQ=255)
                                               ggCTTGCAAAAACTTTATCCACAGCGCCATCGGGACCTTGCGCAGGCGATGATCGACAACGGCAGTG‑CGCTGGTTTCCGAGTATCCCCTGGATGCCGGGCCGCTGCCTGGCAACTTCCCCCGGCGCAACCg                                                                                                                   >  3:143856/1‑131 (MQ=255)
                                               ggCTTGCAAAAACTTTATCCACAGCGCCATCGGGACCTTGCGCAGGCGATGATCGACAACGGCAGTG‑CGCTGGTTTCCGAGTATCCCCTGGATGCCGGGCCGCTGCCTGGCAACTTCCCCCGGCGCAACCg                                                                                                                   <  4:143856/131‑1 (MQ=255)
                                                     cAAAAACTTTATCCACAGCGCCATCGGGACCTTGCGCAGGCGATGATCGACAACGGCAGTG‑CGCTGGTTTCCGAGTATCCCCTGGATGCCGGGCCGCTGCCTGGCAACTTCCCCCGGCGCAACCGCATCATCAGTGGGCTGTCGCTCgg                                                                                           >  5:360276/1‑149 (MQ=255)
                                                      aaaaaCTTTATCCACAGCGCCATCGGGACCTTGCGCAGGCGATGATCGACAACGGCAGTG‑CGCTGGTTTCCGAGTATCCCCTGGATGCCGGGCCGCTGCCTGGCAACTTCCCCCGGCGCAACCGCATCATCCGTGGGCTGTCGCTCGgt                                                                                          >  7:292072/1‑149 (MQ=255)
                                                      aaaaaCTTTATCCACAGCGCCATCGGGACCTTGCGCAGGCGATGATCGACAACGGCAGTG‑CGCTGGTTTCCGAGTATCCCCTGGATGCCGGGCCGCTGCCTGGCAACTTCCCCCGGCGCAACCGCATCATCAGTGGGCTGTCGCTCGgt                                                                                          >  3:149323/1‑149 (MQ=255)
                                                      aaaaaCTTTATCCACAGCGCCATCGGGACCTTGCGCAGGCGATGATCGACAACGGCAGTG‑CGCTGGTTTCCGAGTATCCCCTGGATGCCGGGCCGCTGCCTGGCAACTTCCCCCGGCGCAACCGCATCATCAGTGGGCTGTCGCTCGgt                                                                                          >  1:160515/1‑149 (MQ=255)
                                                      aaaaaCTTTATCCACAGCGCCATCGGGACCTTGCGCAGGCGATGATCGACAACGGCAGTG‑CGCTGGTTTCCGAGTATCCCCTGGATGCCGGGCCGCTGCCTGGCAACTTCCCCCGGCGCAACCGCATCATCAGTGGGCTGTCGCTCGgt                                                                                          <  5:371575/149‑1 (MQ=255)
                                                          aCTTTATCCACAGCGCCATCGGGACCTTGCGCAGGCGATGATCGACAACGGCAGTG‑CGCTGGTTTCCGAGTATCCCCTGGATGCCGGGCCGCTGCCTGGCAACTTCCCCCGGCGCAACCGCATCATCAGTGGGCTGTCGCTCGGTGTGc                                                                                      <  6:175354/149‑1 (MQ=255)
                                                            tttATCCACAGCGCCATCGGGACCTTGCGCAGGCGATGATCGACAACGGCAGTGTCGCTGGTTTCCGATTATCCCATGGATGCCGGGCCGCTGCCTGGCAACTTCCCCCGGCGCAACCGCATCAACAGTGGGCTGTCCCACGGAGTGCt                                                                                     >  1:105621/1‑149 (MQ=255)
                                                            tttATCCACAGCGCCATCGGGACCTTGCGCAGGCGATGATCGACAACGGCAGTG‑CGCTGGTTTCCGAGTATCCCCTGGATGCCGGGCCGCTGCCTGGCAACTTCCCCCGGCGCAACCGCATCATCAGTGGGCTGTCGCTCGGTGTGCtg                                                                                    <  4:180192/149‑1 (MQ=255)
                                                            tttATCCACAGCGCCATCGGGACCTTGCGCAGGCGATGATCGACAACGGCAGTG‑CGCTGGTTTCCGAGTATCCCCTGGATGCCGGGCCGCTGCCTGGCAACTTCCCCCGGCGCAACCGCATCATCAGTGGGCTGTCGCTCGGTGTGCtg                                                                                    >  1:404078/1‑149 (MQ=255)
                                                              tATCCACAGCGCCAGCGGGCCCTTGCGCAGGCGCTGATCGACAACGGGCGTG‑CGCTGGTTTCCGAGGATCCCCTGGCTGCCGGGCCGCTGCCTGGCAACTTCCCCCGGCGCAACCGCATCATCAGTGGGCTGTCGCTCGGTGTGCtg                                                                                    <  5:391736/147‑1 (MQ=255)
                                                               aTCCACAGCGCCATCGGGACCTTGCGCAGGCGATGATCGACAACGGCAGTG‑CGCTGGTTTCCGAGTATCCCCTGGATGCCGGGCCGCTGCCTGGCAACTTCCCCCGGCGCAACCGCATCATCAGTGGGCTGTCGCTCGGTGTGCtggt                                                                                  <  4:148032/148‑1 (MQ=255)
                                                                tCCACAGCGCCATCGGGACCTTGCGCAGGCGATGATCGACAACGGCAGTG‑CGCTGGTTTCCGAGTATCCCCTGGATGCCGGGCCGCTGCCTGGCAACTTCCCCCGGCGCAACCGCATCATCTGTGGGCTGTCGCTCGGTGTGCtggtgg                                                                                <  8:292072/149‑1 (MQ=255)
                                                                tCCACAGCGCCATCGGGACCTTGCGCAGGCGATGATCGACAACGGCAGTG‑CGCTGGTTTCCGAGTATCCCCTGGATGCCGGGCCGCTGCCTGGCAACTTCCCCCGGCGCAACCGCATCATCAGTGGGCTGTCGCTCGGTGTGCtggtgg                                                                                <  1:15097/149‑1 (MQ=255)
                                                                   acaGCGCCATCGGGACCTTGCGCAGGCGATGATCGACAACGGCAGTG‑CGCTGGTTTCCGAGTATCCCCTGGATGCCGGGCCGCTGc                                                                                                                                            <  7:341234/86‑1 (MQ=255)
                                                                   acaGCGCCATCGGGACCTTGCGCAGGCGATGATCGACAACGGCAGTG‑CGCTGGTTTCCGAGTATCCCCTGGATGCCGGGCCGCTGc                                                                                                                                            >  8:341234/1‑86 (MQ=255)
                                                                   acaGCGCCATCGGGACCTTGCGCAGGCGATGATCGACAACGGCAGTG‑CGCTGGTTTCCGAGTATCCCCTGGATGCCGGGCCGCTGCCTGGCAACTTCCCCCGGCGCAACCGCATCATCAGTGGGCTGTCGCTCGGTGTGCTGGTGGTc                                                                              >  5:79404/1‑148 (MQ=255)
                                                                    caGCGCCATCGGGACCTTGCGCAGGCGATGATCGACAACGGCAGTG‑CGCTGGTTTCCGAGTATCCCCTGGATGCCGGGCCGCTGCCTGGCAACTTCCCCCGGCGCAACCGCATCATCAGTGGGCTGTCGCTCGGTGTGCTGGTGGTCGa                                                                            <  4:149323/149‑1 (MQ=255)
                                                                     aGCGCCATCGGGACCTTGCGCAGGCGATGATCGACAACGGCAGTG‑CGCTGGTTTCCGAGTATCCCCTGGATGCCGGGCCGCTGCCTGGCAACTTCCCCCGGCGCAACCGCATCATCAGTGGGCTGTCGCTCGGTGTGCTGGTGGTCGAg                                                                           <  2:134682/149‑1 (MQ=255)
                                                                     aGCGCCATCGGGACCTTGCGCAGGCGATGATCGACAACGGCAGTG‑CGCTGGTTTCCGAGTATCCCCTGGATGCCGGGCCGCTGCCTGGCAACTTCCCCCGGCGCAACCGCATCATCAGTGGGCTGTCGCTCGGTGTGCTGGTGGTCGAg                                                                           <  6:310944/149‑1 (MQ=255)
                                                                        gcCATCGGGACCTTGCGCAGGCGATGATCGACAACGGCAGTG‑CGCTGGTTTCCGAGTATCCCCTGGATGCCGGGCCGCTGCCTGGCAACTTCCCCCGGCGCAACCGCATCATCAGTGGGCTGTCGCTCGGTGTGCTGGTGG‑CGAGGctt                                                                       <  6:360276/149‑3 (MQ=255)
                                                                              gggACCTTGCGCAGGCGATGATCGACAACGGCAGTG‑CGCTGGTTTCCGAGTATCCCCTGGATGCCGGGCCGCTGCCTGGCAACTTCCCCCGGCGCAACCGCATCATCAGTGGGCTGTCGCTCGGTGTGCTGGTGGTCGAGGCAAGCCTc                                                                  <  3:197225/149‑1 (MQ=255)
                                                                              gggACCTTGCGCAGGCGATGATCGACAACGGCAGTG‑CGCTGGTTTCCGAGTATCCCCTGGATGCCGGGCCGCTGCCTGGCAACTTCCCCCGGCGCAACCGCATCATCAGTGGGCTGTCGCTCGGTGTGCTGGTGGTCGAGGCAAGCCTc                                                                  <  1:380306/149‑1 (MQ=255)
                                                                                  ccTTGCGCAGGCGATGATCGACAACGGCAGTG‑CGCTGGTTTCCGAGTATCCCCTGGATGCCGGGCCGCTGCCTGGCAACTTCCCCCGGCGCAACCGCATCATCAGTGGGCTGTCGCTCGGTGTGCTGGTGGTCGAGGCAAGCCTCGc                                                                >  8:234318/1‑147 (MQ=255)
                                                                                   cTTGCGCAGGCGATGATCGACAACGGCAGTG‑CGCTGGTTTCCGAGTATCCCCTGGATGCCGGGCCGCTGCCTGGCAACTTCCCCCGGCGCAACCGCATCATCAGTGGGCTGTCGCTCGGTGTGCTGGTGGTCGAGGCAAGCCTCGCCAg                                                             <  3:61788/149‑1 (MQ=255)
                                                                                   cTTGCGCAGGCGATGATCGACAACGGCAGTG‑CGCTGGTTTCCGAGTATCCCCTGGATGCCGGGCCGCTGCCTGGCAACTTCCCCCGGCGCAACCGCATCATCAGTGGGCTGTCGCTCGGTGTGCTGGTGGTCGAGGCAAGCCTCGCCAg                                                             <  2:105621/149‑1 (MQ=255)
                                                                                      gcgcAGGCGATGATCGACAACGGCAGTG‑CGCTGGTTTCCGAGTATCCCCTGGATGCCGGGCCGCTGCCTGGCAACTTCCCCCGGCGCAACCGCATCATCAGTGGGCTGTCGCTCGGTGTGCTGGTGGTCGAGGCAAGCCTCGCCAGCgg                                                          >  5:405603/1‑149 (MQ=255)
                                                                                                 gatCGACAACGGCAGTG‑CGCTGGTTTCCGAGTATCCCCTGGATGCCGGGCCGCTGCCTGGCAACTTCCCCCGGCGCAACCGCATCATCAGTGGGCTGTCGCTCGGTGTGCTGGTGGTCGAGGCAAGCCTCGCCAGCGGCTCGCTGATCa                                               >  6:111879/1‑149 (MQ=255)
                                                                                                   tcgtaaaCGCCAGTG‑CGCTGTTATCCGAGTATCCCCTGGATGCCGGGCCGCTGCCTTGCAACTTCCCCCGGCGCAACCCCATCATCAGTGGGCTGTCGCTCGGTGTGCTGGTGGTCGAGGCAATCCTCGCCAGCGGCTCGCTGATCACg                                             <  8:365418/144‑1 (MQ=255)
                                                                                                   tCGACAACGGCAGTG‑CGCTGGTTTCCGAGTATCCCCTGGATGCCGGGCCGCTGCCTGGCAACTTCCCCCGGCGCAACCGCATCATCAGTGGGCTGTCGCTCGGTGTGCTGGTGGTCGAGGCAAGCCTCGCCAGCGGCTCGCTGATCACg                                             >  2:357394/1‑149 (MQ=255)
                                                                                                     gACAACGGCAGTG‑CGCTGGTTTCCGAGTATCCCCTGGATGCCGGGCCGCTGCCTGGCAACTTCCCCCGGCGCAACCGCATCATCAGTGGGCTGTCGCTCGGTGTGCTGGTGGTCGAGGCAAGCCTCGCCAGCGGCTCGCTGATCACGgc                                           <  6:79404/149‑1 (MQ=255)
                                                                                                     gACAACGGCAGTG‑CGCTGGTTTACGAGTATCCCCTGGATGCCGGGCCGCTGCCTGGCAACTTCCCCCGGCGCAACCGCATCATCAGTGGGCTGTCGCTCGGTGTGCTGGTGGTCGAGGCAAGCCTCGCCAGCGGCTCGCTGATCACgg                                            <  6:405603/148‑1 (MQ=255)
                                                                                                                      tGGTTTCCGAGTATCGCCTGGATGCCGGGTCGCTGGCTGTCAACTTCCCACGGCGCAACCGGATATTCAGTGGGCTGTCGCTCGGTGTGCTGGTGGTCGATGCAAGCGTCGCCAGCGGGTCTCTGATGACGGCTCGTCTGGCGGCGGaa                           <  8:385287/149‑1 (MQ=255)
                                                                                                                        gTTTCCGAGTATCCCCTGGATGCCGGGCCGCTGCCTGGCAACTTCCCCCGGCGCAACCGCATCATCAGTGGGCTGTCGCTCGGTGTGCTGGTGGTCGAGGCAAGCCTCGCCAGCGGCTCGCTGATCACGGCGCGTCTGGCGGCGGaa                           <  5:111879/147‑1 (MQ=255)
                                                                                                                         tttCCGAGTATCCCCTGGATGCCGGGCCGCTGCCTGGCAACTTCCCCCGGCGCAACCGCATCATCAGTGGGCTGTCGCTCGGTGTGCTGGTGGTCGAGGCAAGCCTCGCCAGCGGCTCGCTGATCACGGCGCGTCTGGCGGCGGAACaa                        <  2:404078/149‑1 (MQ=255)
                                                                                                                          ttCCGAGTATCCCCTGGATGCCGGGCCGCTGCCTGGCAACTTCCCCCGGCGCAACCGCATCATCAGTGGGCTGTCGCTCGGTGTGCTGGTGGTCGAGGCAAGCCTCGCCAGCGGCTCGCTGATCACGGCGCGTCTGGCGGCGGAACAAg                       >  2:257568/1‑149 (MQ=255)
                                                                                                                          ttCCGAGTATCCCCTGGATGCCGGGCCGCTGCCTGGCAACTTCCCCCGGCGCAACCGCATCATCAGTGGGCTGTCGCTCGGTGTGCTGGTGGGCGAGGctt                                                                       >  2:267109/1‑99 (MQ=255)
                                                                                                                          ttCCGAGTATCCCCTGGATGCCGGGCCGCTGCCTGGCAACTTCCCCCGGCGCAACCGCATCATCAGTGGGCTGTCGCTCGGTGTGCTGGTGGGCGAGGctt                                                                       <  1:267109/101‑3 (MQ=255)
                                                                                                                           tCCGAGTATCCCCTGGATGCCGGGCCGCTGCCTGGCAACTTCCCCCGGCGCAACCGCATCATCAGTGGGCTGTCGCTCGGTGTGCTGGTGGTCGAGGCAAGCCTCGCCAGCGGCTCGCTGATCACGGCGCGTCTGGCGGCGGAACAAg                       <  7:234318/148‑1 (MQ=255)
                                                                                                                           tCCGAGTATCCCCTGGATGCCGGGCCGCTGCCTGGCAACTTCCCCCGGCGCAACCGCATCATCAGTGGGCTGTCGCTCGGTGTGCTGGTGGTCGAGGCAAGCCTCGCCAGCGGCTCGCTGATCACGGCGCGTCTGGCGGCGGAACAAg                       <  1:357394/148‑1 (MQ=255)
                                                                                                                           tCCGAGTATCCCCTGGATGCCGGGCCGCTGCCTGGCAACTTCACCCGGCGCAACCGCATCATCAGTGGGCTGTCGCTCGGTGTGCTGGTGGTCGAGGCAAGCCTCGCCAGCGGCTCGCTGATCACGGCGCGTCTGGCGGCGGAACAAgg                      >  6:174504/1‑149 (MQ=255)
                                                                                                                                    ccccTGGATGCCGGGCCGCTGCCTGGCAACTTCCCCCGGCGCAACCGCATCATCAGTGGGCTGTCGCTCGGTGTGCTGGTGGTCGAGGCAAGCCTCGCCAGCGGCTCGCTGATCACGGCGCGTCTGGCGGCGGAACAAGGCCGCGAGgt             >  3:377194/1‑149 (MQ=255)
                                                                                                                                      ccTGGATGCCGGGCCGCTGCCTGGCAACTTCCCCCGGCGCAACCGCATCATCAGTGGGCTGTCGCTCGGTGTGCTGGTGGTCGAGGCAAGCCTCGCCAGCGGCTCGCTGATCACGGCGCGTCTGGCGGCGGAACAAGGCCGCGAGgtg            >  6:398781/1‑148 (MQ=255)
                                                                                                                                       cTGGATGCCGGGCCGCTGCCTGGCAACTTCCCCCGGCGCAACCGCATCATCAGTGGGCTGTCGCTCGGTGTGCTGGTGGTCGAGGCAAGCCTCGCCAGCGGCTCGCTGATCACGGCGCGTCTGGCGGCGGAACAAGGCCGCGAGGTGTa          >  2:18964/1‑149 (MQ=255)
                                                                                                                                        tGGATGCCGGGCCGCTGCCTGGCAACTTCCCCCGGCGCAACCGCATCATCAGTGGGCTGTCGCTCGGTGTGCTGGTGGTCGAGGCAAGCCTCGCCAGCGGCTCGCTGATCACGGCGCGTCTGGCGGCGGAACAAGGCCGCGAGGTGTAc         >  8:195797/1‑149 (MQ=255)
                                                                                                                                               cGGGCCGCTGCCTGGCAACTTCCCCCGGCGCAACCGCATCATCAGTGGGCTGTCGCTCGGTGTGCTGGTGGTCGAGGCAAGCCTCGCCAGCGGCTCGCTGATCACGGCGCGTCTGGCGGCGGAACAAGGCCGCGAGGTGTACGCCATTc  >  4:132244/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                               
GGGCCGCGTTGCAGGCTGGCGGCGGCACGATTGCCGTGCTCGGTACGGGCTTGCAAAAACTTTATCCACAGCGCCATCGGGACCTTGCGCAGGCGATGATCGACAACGGCAGTG‑CGCTGGTTTCCGAGTATCCCCTGGATGCCGGGCCGCTGCCTGGCAACTTCCCCCGGCGCAACCGCATCATCAGTGGGCTGTCGCTCGGTGTGCTGGTGGTCGAGGCAAGCCTCGCCAGCGGCTCGCTGATCACGGCGCGTCTGGCGGCGGAACAAGGCCGCGAGGTGTACGCCATTC  >  NC_002947/79748‑80038

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑