| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,459,560 | T→G | 7.8% | intergenic (+18/‑22) | PP_4795 → / → holA | lipoprotein/DNA polymerase III subunit delta |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,459,560 | 0 | T | G | 7.8% | 130.7 / 4.6 | 52 | intergenic (+18/‑22) | PP_4795/holA | lipoprotein/DNA polymerase III subunit delta |
| Reads supporting (aligned to +/- strand): ref base T (19/26); new base G (2/2); total (24/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.93e-01 | |||||||||||
CCCAACTGGATGAGCTGCAGCGTAAAGCAGACGAGCGCGCCCAAGCCGAAGCTGCCGCGCTGGAGGCTGCCCGCCGCCAGCAAGCCGAGACGCCTCAGCAATCGCCACTGGAAGTACCGGGCAACTAAGCCCGCGAGGGGCGCCCTCGGGCGCCCCGCCTGTTCCCCAT‑GAAACT‑CGCCCCCGCCCAACTCAACAAGCACCTGCAAGGCGCCCTGGCACCGGTCTACGTGGTCAGTGGCGACGACCCGCTGCTGTGCCAGGAAGCCGCCGACGCCATCCGCAATG > NC_002947/5459415‑5459699 | cccAACTGGATGAGCTGCAGCGTAAAGCAGACGAGCGCGCCCAAGCCGAAGCTGCCGCGCTGGAGGCTGCCCGCCGCCAGCAAGCCGAGACGCCTCAGCAATCGCCACTGGAAGTACCGGGCAACTAAGCCCGCGAGGGGCGCCCTCgg < 4:266798/149‑1 (MQ=255)cccAACTGGATGAGCTGCAGCGTAAAGCAGACGAGCGCGCCCAAGCCGAAGCTGCCGCGCTGGAGGCTGCCCGCCGCCAGCAAGCCGAGACGCCTCAGCAATCGCCACTGGAAGTACCGGGCAACTAAGCCCGCGAGGGGCGCCCTCgg < 2:332361/149‑1 (MQ=255) ccAACTGGATGAGCTGCAGCGTAAAGCAGACGAGCGCGCCCAAGCCGAAGCTGCCGCGCTGGAGGCTGCCCGCCGCCAGCAAGCCGAGACGCCTCAGCAATCGCCACTGGAAGTACCGGGCAACTAAGCCCGCGAGGGGCGCCCTCggg > 4:363301/1‑149 (MQ=255) aaCTGGATGAGCTGCAGCGTAAAGCAGACGAGCGCGCCCAAGCCGAAGCTGCCGCGCTGGAGGCTGCCCGCCGCCAGCAAGCCGAGACGCCTCAGCAATCGCCACTGGAAGTACCGGGCAACTAAGCCAGCGAGGGGCGCCCTCggg > 3:299519/1‑147 (MQ=255) aCTGGATGAGCTGCAGCGTAAAGCAGACGAGCGCGCCCAAGCCGAAGCTGCCGCGCTGGAGGCTGCCCGCCGCCAGCAAGCCGAGACGCCTCAGCAATCGCCACTGGAAGTACCGGGCAACTAAGCCCGCGAGGGGCGCCCGAGGgcgc < 2:166394/149‑1 (MQ=255) attaGCTTCATCGTCACGCAGACGAGCGCTCCCAAGCCGAAGCTGACGCGCTGGAGGATGCCCGCCGCCAGCAAGCCGAGACGCCTCAGCAATCGCCACTGGAAGTACCGGGCAACTAAGCCCGCGAGGGGCGCCCGAGGGCGcccctc < 4:304710/146‑3 (MQ=255) aTGAGCTGCAGCGTAAAGCAGACGAGCGCGCCCAAGCCGAAGCTGCCGCGCTGGAGGCTGCCCGCCGCCAGCAAGCCGAGACGCCTCAGCAATCGCCACTGGAAGTACCGGGCAACTAAGCCCGCGAGGGGCGCCCTCGGGCGCCCCGc > 7:333588/1‑149 (MQ=255) aGCTGCAGCGTAAAGCAGACGAGCGCGCCCAAGCCGAAGCTGCCGCGCTGGAGGCTGCCCGCCGCCAGCAAGCCGAGACGCCTCAGCAATCGCCACTGGAAGTACCGGGCAACTAAGCCCGCGAGGGGCGCCCTCGGGCGCCCCGCCt < 3:1815/148‑1 (MQ=255) aGCTGCAGCGTAAAGCAGACGAGCGCGCCCAAGCCGAAGCTGCCGCGCTGGAGGCTGCCCGCCGCCAGCAAGCCGAGACGCCTCAGCAATCGCCACTGGAAGTACCGGGCAACTAAGCCCGCGAGGGGCGCCCTCGGGCGCCCCGCCTg < 2:43877/149‑1 (MQ=255) cTGCAGCGTAAAGCAGACGAGCGCGCCCAAGCCGAAGCTGCCGCGCTGGAGGCTGCTCGCCGCCAGCAAGCCGAGACGCCTCAGCAATCGCCACTGGAAGTACCGGGCAACTAAGCCCGCGAGGGGCGCCCTCGGGCGCCCCGCCTGtt > 1:156710/1‑149 (MQ=255) cAGCGTAAAGCAGACGAGCGCGCCCAAGCCGAAGCTGCCGCGCTGGAGGCTGCCCGCCGCCAGCAAGCCGAGACGCCTCAGCAATCGCCACTGGAAGTACCGGGCAACTAAGCCCGCGAGGGGCGCCCTCGGGCGCCCCGCCTGTTc < 8:333588/147‑1 (MQ=255) cGTAAAGCAGACGAGCGCGCCCAAGCCGAAGCTGCCGCGCTGGAGGCTGCCCGCCGCCAGCAAGCCGAGACGCCTCAGCAATCGCCACTGGAAGTACCGGGCAACTAAGCCCGCGAGGGGCGCCCTCGGGCGCCCCGCCTGTTCCCCAt < 8:64944/149‑1 (MQ=255) aCGAGCGCGCCCAAGCCGAAGCTGCCGCGCTGGAGGATGCCCGCCGCCAGCAAGCCGAGACGCCTCAGCAATCGCCACTGGAAGTACCGGGCAACTAAGCCCGCGAGGGGCGCCCACGGGCGCCCCGCCAGTTGCCCAT‑GAAAcacccc > 1:270579/1‑144 (MQ=255) cGAGCGCGCCCAAGCCGAAGCTGCCGCGCTGGAGGCTGCCCGCCGCCAGCAAGCCGAGACGCCTCAGCAATCGCCACTGGAAGTACCGGGCAACTAAGCCCGCGAGGGGCGCCCTCGGGCGCCCCGCCGGGTCCCCAA‑GAAACT‑CGccc > 6:43901/1‑149 (MQ=255) cgcgcCCAAGCCGAAGCTGCCGCGCTGGAGGCTGCTCGCCGCCAGCAAGCCGAGACGCCTCAGCAATCGCCACTGGAAGTACCGGGCAACTAAGCCCGCGAGGGGCGCCCTCGGGCGCCCCGCCTGTTCCCCAT‑GAAACT‑CGCCCCCGc < 2:156710/149‑1 (MQ=255) aaGCCGAAGCTGCCGCGCTGGAGGCTGCCCGCCGCCAGCAAGCCGAGACGCCTCAGCAATCGCCACTGGAAGTACCGGGCAACTAAGCCCGCGAGGGGCGCCCTCGGGCGCCCCGCCTGTTCCCCAT‑GAAACT‑CGCCCCCGCCCAACt > 2:26191/1‑148 (MQ=255) gCCGAAGCTGCCGCGCTGGAGGCTGCCCGCCGCCAGCAAGCCGAGACGCCTCAGCAATCGCCACTGGAAGTACCGGGCAACTAAGCCCGCGAGGGGCGCCCTCGGGCGCCCCGCCTGGTCCCCAA‑TCAACT‑CGCCCCCCCCCAAcgaac > 8:321022/1‑145 (MQ=255) gCCGAAGCTGCCGCGCTGGAGGCTGCCCGCCGCCAGCAAGCCGAGACGCCTCAGCAATCGCCACCGGAAGTACCGGGCAACTAAGCCCGCGAGGGGCGCCCTCGGGCGCCCCGcc > 7:82297/1‑115 (MQ=255) gCCGAAGCTGCCGCGCTGGAGGCTGCCCGCCGCCAGCAAGCCGAGACGCCTCAGCAATCGCCACCGGAAGTACCGGGCAACTAAGCCCGCGAGGGGCGCCCTCGGGCGCCCCGcc < 8:82297/115‑1 (MQ=255) gAAGCTGCCGCGCTGGAGGCTGCCCGCCGCCAGCAAGCCGAGACGCCTCAGCAATCGCCACTGGAAGTACCGGGCAACTAAGCCCGCGAGGGGCGCCCTCGGGCGCCCCGCCTGTTCCCCAT‑GAAACT‑CGCCCCCGCCCAACTcaacaa < 3:165980/149‑1 (MQ=255) gAAGCTGCCGCGCTGGAGGCTGCCCGCCGCCAGCAAGCCGAGACGCCTCAGCAATCGCCACTGGAAGTACCGGGCAACTAAGCCCGCGAGGGGCGCCCTCGGGCGCCCCGCCTGTTCCCCAT‑GAAACT‑CGCCCCCGCCCAACTcaacaa < 7:321022/149‑1 (MQ=255) aGCTGCCGCGCTGGAGGCTGCCCGCCGCCAGCAAGCCGAGACGCCTCAGCAATCGCCACTGGAAGTACCGGGCAACTAAGCCCGCGAGGGGCGCCCTCGGGCGCCCCGCCTGTTCCCCAT‑GAAACT‑CGCCCCCGCCCAACTCAACAAGc < 1:170970/149‑1 (MQ=255) cTGCCGCGCTGGAGGCTGCCCGCCGCCAGCAAGCCGAGACGCCTCAGCAATCGCCACTGGAAGTACCGGGCAACTAAGCCCGCGAGGGGCGCCCTCGGGCGCCCCGCCTGTTCCCCAT‑GAAACT‑CGCCCCCGCCCAACTCAACAAGCAc < 5:92982/149‑1 (MQ=255) cgcTGGAGGCAGCCCGACGACAGCAAGCCCATCGCCCGCAGAAATCTCCCATGGAAGTACCGGGCAACAAAGCCCGCGAGGGGCGCCCTCGGGCGCCCAGCCTGTTCCCCAT‑GAAACT‑CGCCCCCGCCCAACTCAACAATCACCTGCaa < 4:304758/149‑1 (MQ=255) gAGGCGGCCCGCCGCCAGCAAGCCGAGACGCCTCAGCAATCGCCCCTGGATGTACCGGGCAACTAAGCCCGCGAGGGGCGCCCTCGGGCGCCCCGCCTGTTCCCCAT‑GAAACT‑CGCCCCCGCCCAACTCAACAAGCACCTGCAAGgcgc < 2:337209/149‑1 (MQ=255) tGCCCGCCGCCAGCAAGCCGAGACGCCTCAGCAATCGCCACTGGAAGTACCGGGCAACTAAGCCCGCGAGGGGCGCCCTCGGGCGCCCCGCCTGTTACCCAT‑GAAACT‑CGCCCCCGCCCAACTCAACAACCACCTGCAAGGCGCCCTgg > 6:97824/1‑149 (MQ=255) cgccgcCAGCAAGCCGAGACGCCTCAGCAATCGCCACTGGAAGTACCGGGCAACTAAGCCCGCGAGGGGCCCCCACGGGCGCCCCCCCGGTTCACCAA‑GAAAAA‑ACCCCCCGCCCAACAAAACAACGACCAGCAAAGCCCCCAGGccac > 4:166842/1‑146 (MQ=255) aGCCGAGACGCCTCAGCAATCGCCACTGGAAGTACCGGGCAACTAAGCCCGCGAGGGGCGCCCTCGGGCGCCCCGCCTGTTCCCCAT‑GAAACT‑CGCCCCCGCCCAACTCAACAAGCACCTGCAAGGCGCCCTGGCACCGGTCTACGTgg < 5:143775/149‑1 (MQ=255) gagaCGCCTCAGCAATCGCCACTGGAAGTACCGGGCAACTAAGCCCGCGAGGGGCGCCCTCGGGCGCCCCGCCTGTTCCCCAT‑GAAACT‑CGCCCCCGCCCAACTCAACAAGCACCTGCAAGGCGCCCTGGCACCGGTCTACGTGGTCAg < 3:166842/149‑1 (MQ=255) gagaCGCCTCAGCAATCGCCACTGGAAGTACCGGGCAACTAAGCCCGCGAGGGGCGCCCACGGGCGCCCCGCCAGTTGCCCAA‑AAAACTACCCCCCCCCCCAAGACAA‑AACCACCTGCACAGCCCCCAGGCCCCCGGCGACCTGGGCAg > 8:142792/1‑149 (MQ=255) agaCGCCTCAGCAATCGCCACTGGAAGTACCGGGCAACTAAGCCCGCGAGGGGCGCCCTCGGGCGCCCCGCCTGTTCCCCAT‑GAAACT‑CGCCCCCGCCCAACTCAACAAGCACCTGCAAGGCGCCCTGGCACCGGTCTACGTGGTCAGt < 3:363301/149‑1 (MQ=255) taCGCCTCAGCAATCGCCACTGGAAGTACCGGGCAACTAAGCCCGCGAGGGGCGCCCTCGGGCGCCCCGCCTGTTCCCCAT‑GAAACT‑CGCCCCCGCCCAACTCAACAAGCACCTGCAAGGCGCCCTGGCACCGGTCTACGTGGTCAGTg < 7:255591/148‑1 (MQ=255) gCCTCAGCAATCGCCACTGGAAGTACCGGGCAACTAAGCCCGCGAGGGGCGCCCTCGGGCGCCCCGCCTGTTCCCCAT‑GAAACT‑CGCCCCCGCCCAACTCAACAAGCACCTGCAAGGCGCCCTGGCACCGGTCTACGTGGTCa < 5:369905/143‑1 (MQ=255) gCCTCAGCAATCGCCACTGGAAGTACCGGGCAACTAAGCCCGCGAGGGGCGCCCTCGGGCGCCCCGCCTGGTGCCCATGGAAAAA‑CGCCCCC‑CCCAACAAAACAACCACCGGCAAGGCACCCGGGCACCGGGATAAGGGGTCaaagaac > 6:369905/1‑143 (MQ=255) ccTCAGCAATCGCCACTGGAAGTACCGGGCAACTAAGCCCGCGAGGGGCGCCCTCGGGCGCCCCGCCTGTTCCCCAT‑GAAACT‑CGCCCCCGCCCAACTCAACAAGCACCAGCAAGGCGCCCTGGGACCGGGATACGTGGTCAGTGGcg > 8:145726/1‑148 (MQ=255) tCAGCAATCGCCACTGGAAGTACCGGGCAACTAAGCCCGCGAGGGGCGCCCTCGGGCGCCCCGCCTGTTCCCCAT‑GAAACT‑CGCCCCCGCCCAACGCAACCAGCCCCAGCAAGGCGCCCTGGCACCGGTCTACGTGGTCAGTGGcgac > 2:99830/1‑148 (MQ=255) tCAGCAATCGCCACTGGAAGTACCGGGCAACTAAGCCCGCGAGGGGCGCCCTCGGGCGCCCCGCCTGTTCCCCAT‑GAAACT‑CGCCCCCGCCCAACGCAACAAGCACCTGCAAGGCGCCCAGGCCCCGGTCTACGTGGGCAGTGGcgacg > 7:191702/1‑149 (MQ=255) aGCAATCGCCACTGGAAGTACCGGGCAACTAAGCCCGCGAGGGGCGCCCTCGGGCGCCCCGCCTGTTGCCCAT‑GCAACA‑CGCCCCCGCCCAACACAACAAGCACCAGCCAGGCCCCCATGCACCgggcgac > 4:316552/1‑126 (MQ=255) aGCAATCGCCACTGGAAGTACCGGGCAACTAAGCCCGCGAGGGGCGCCCTCGGGCGCCCCGCCTGTTCCCCAT‑GAAACT‑CGCCCCCGCCCAACTCAACAAGCACCTGCAAGGCGCCCTGGCACCGGTCTAc < 3:316552/131‑1 (MQ=255) ccACTGGAAGTACCGGGCAACTAAGCCCGCGAGGGGCGCCCTCGGGCGCCCCGCCTGTTCCCCAA‑GCAACT‑CGCCCCCGCCCCAAGCCACAAGCACCTGCCAGGCGCCCTGGCACCGGTCTTACTGGTCAGTGGCGGCGCCCCgcggct > 5:191696/1‑149 (MQ=255) gAAGTACCGGGCAACTAAGCCCGCGAGGGGCGCCCTCGGGCGCCCCGCCTGTTGCCCCA‑GAAACA‑AGCCCCCGCCCAACACAACAAGCACCAGCAAGGCGCCCAGGCACCGGTGAACCTGGTCAGTGGCGCCCACCCGCGGCTGTGc > 2:153862/1‑147 (MQ=255) gAAGTACCGGGCAACTAAGCCCGCGAGGGGCGCCCTCGGGCGCCCCGCCTGTTCCCCAT‑GAAACT‑CGCCCCCGCCCAACTCAACAAGCACCTGCAAGGCGCCCTGGCACCGGTCTACGTGGTCAGTGGCGACGACCCGCTGCTGTGCCa < 5:43901/149‑1 (MQ=255) ccGGGCAACTAAGCCCGCGAGGGGCGCCCTCGGGCGCCCCGCCTGTTCCCCAT‑GAAACT‑CGCCCCCGCCCAACTCAACAAGCACCTGCAAGGCGCCCTGGCACCGGTCTACGTGGTCAGTGGCGACGACCCGCTGCTGTGCCa < 5:157909/143‑1 (MQ=255) ccGGGCAACTAAGCCCGCGAGGGGCGCCCTCGGGCGCCCCGCCTGTTACCCAT‑GAAACT‑CGCCCCCGCCCAACTAAACAAGCACCTGCAAGGCGCCCTGGCACCGGTCGACGTGGTCACTGGCGACGACCCCCTGCTGTGCCa > 6:157909/1‑143 (MQ=255) gggCAACTACGCCCGCGAGGGGCGCCCTCGGGCGCCCCGCCTGTTCCCCAT‑GAAACT‑CGCCCCCGCCCAACTCAACAAGCACCTGCAAGGCGCCCTGGCACCGGTCTACGTGGTCAGTGGCGACGACCCGCTGCTGTGCCAGGAAgccg < 4:255125/149‑1 (MQ=255) ggCAACTAAGCCCGCGAGGGGCGCCCTCGGGCGCCCCGCCTGTTCCCCAT‑GAAACT‑CGCCCCCGCCCAACTCAACAAGCACCTGCAAGGCGCCCTGGCACCGGTCTACGTgg < 1:196403/112‑1 (MQ=255) cTAAGCCCGCGAGGGGCGCCCTCGGGCGCCCCGCCTGTTCCCCAT‑GAAACT‑CGCCCCCGCCCAACTCAACAGGCACCTGCAAGGCGCCCAGGCACCGGTCTACGTGGGCAGTGGGGACGCCCCGCGGCTGTGCCAGGAAGccgccgccg > 5:352870/1‑149 (MQ=255) ccGCGAGGGGCGCCCTCGGGCGCCCCGCCTGTTCCCCAT‑GAAACT‑CGCCCCCGCCCAACTCAACAAGCACCTGCAAGGCGCCCTGGCACCGGTCTACGTGGTCAGTGGCGACGACCCGCTGCTGTGCCAGGAAgccgcc > 4:321935/1‑139 (MQ=255) ccGCGAGGGGCGCCCTCGGGCGCCCCGCCTGTTCCCCAT‑GAAACT‑CGCCCCCGCCCAACTCAACAAGCACCTGCAAGGCGCCCTGGCACCGGTCTACGTGGTCAGTGGCGACGACCCGCTGCTGTGCCAGGAAgccgcc < 3:321935/139‑1 (MQ=255) ggcggggCGCCCGAGGGCGCCCCTCCTGTTCCCCAT‑GAAACT‑CGCCCCCGCCCAACTCAACAAGCACCTGCAAGGCGCCCTGGCCCCGGTCTACGTGGTCAGTGGCGCCGCCCCGCTGCTGTGCCAGGAAGCCGCCGACGCCATCCGc > 5:319372/4‑148 (MQ=255) cggggCGCCCGAGGGCGCCCCGCCTGTTCCCCAT‑GAAACT‑CGCCCCCGCCCAACTCAACAAGCACCGGCAACGCCCCCTGGCACCGGGCTACCTGGTCAGTGGCGACGACCCCCTGCTGTGCCAGGAAGCCGCCGACCCCAACCGCCAt > 8:52074/2‑149 (MQ=255) ggggCGCCCTCGGGCGCCCCGCCTGTTCCCCAT‑GAAACT‑CGCCCCCGCCCAACTCAACAAGCACCTGCAAGGCGCCCTGGCACCGGTCTACGTGGTCAGTGGCGACGACCCGCTGCTGTGCCAGGAAGCCGCCGACGCCATCCGCAATg < 2:270579/149‑1 (MQ=255) | CCCAACTGGATGAGCTGCAGCGTAAAGCAGACGAGCGCGCCCAAGCCGAAGCTGCCGCGCTGGAGGCTGCCCGCCGCCAGCAAGCCGAGACGCCTCAGCAATCGCCACTGGAAGTACCGGGCAACTAAGCCCGCGAGGGGCGCCCTCGGGCGCCCCGCCTGTTCCCCAT‑GAAACT‑CGCCCCCGCCCAACTCAACAAGCACCTGCAAGGCGCCCTGGCACCGGTCTACGTGGTCAGTGGCGACGACCCGCTGCTGTGCCAGGAAGCCGCCGACGCCATCCGCAATG > NC_002947/5459415‑5459699 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |