| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,485,168 | C→T | 20.0% | intergenic (+74/‑73) | purH → / → purD | bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase/phosphoribosylamine‑‑glycine ligase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,485,168 | 0 | C | T | 20.0% | 51.6 / 6.3 | 25 | intergenic (+74/‑73) | purH/purD | bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase/phosphoribosylamine‑‑glycine ligase |
| Reads supporting (aligned to +/- strand): ref base C (13/7); new base T (2/3); total (15/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.58e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TCGCTGCTGCCGACGAGGCCGGCATCGCGATGGTCTTCACTGGCATGCGCCACTTCCGCCACTAATTACGCGGATCCCGAGGCAAGTCGAGATC‑CTGTGGGAGCGGGCTTGCCCGCGAATACGATGGTGGATTCACCGCCGCATTCGCGGGCAAGCCCGCTCCCACAGTGTTCGGCGCAAGCCACGGGATCACTTGAATCGAGGTTTTGACATGAAAGTTTTGATCATCGGCAGCGGTGGCCGTGAGCACGCCCTGGCCTGGAAAGTCGCCCAGGACCCACGCGT > NC_002947/5485030‑5485314 | tCGCTGCTGCCGACGAGGCCGGCATCGCGATGGTCTTCACTGGCATGCGCCACTTCCGCCACTAATTACGCGGATCCCGTGGCTTGCGCCGAACACTGTGGGAGCGGGCTTGCCCGCGAATGCGGCGGTGAATCCACCATCGTATTcgc < 3:372847/149‑1 (MQ=14) gctgctGCCGACGAGGCCGGCATCGCGATGGTCTTCACTGGCATGCGCCACTTCCGCCACTAATTACGCGGATCCCGAGGCAAGTTGAGATC‑CTGTGGGAGCGGGCTTGCCCGCGAATGCGGCGGTGAATCCACCATCGTATTCGCgg < 6:285029/148‑1 (MQ=25) gctgctGCCGACGAGGCCGGCATCGCGATGGTCTTCACTGGCATGCGCCACTTCCGCCACTAATTACGCGGATCCCGAGGCAAGTCGAGATC‑CTGTGGGAGCGGGCTTGCCCGCGAATGCGGCGGTGAATCCACCATCGTATTCGCgg < 3:10703/148‑1 (MQ=37) gctgctGCCGACGAGGCCGGCATCGCGATGGTCTTCACTGGCATGCGCCACTTCCGCCACTAATTACGCGGATCCCGAGGCAAGTCGAGATC‑CTGTGGGAGCGGGCTTGCCCGCGAATACGATGGTGGATTCACCGCCGCATTCGCgg < 2:242928/148‑1 (MQ=255) gctgctGCCGACGAGGCCGGCATCGCGATGGTCTTCACTGGCATGCGCCACTTCCGCCACTAATTACGCGGATCCCGAGGCAAGTCGAGATC‑CTGTGGGAGCGGGCTTGCCCGCGAATACGATGGTGGATTCACCGCCGCATTCGCgg < 6:258263/148‑1 (MQ=255) ctgctgCCGACGAGGCCGGCATCGCGATGGTCTTCACTGGCATGCGCCACTTCCGCCACTAATTACGCGGATCCCGAGGCAAGTCGAGAGC‑CTGTGGGAGCGGGCTTGCCCGCGAATACGATGGTGGATTCACCGCCGCATTCGCGGGc < 1:8377/149‑1 (MQ=255) ggCTTGCCCGCGAATACGATGGTGGATTCACCGCCGCATTCGCGGGCAAGCCCGCTCCCACAGTGTTCGGCGCAAGCCACGGGATCACTTGAATCGAGGTTTTGACATGAAAGTTTTGATCATCGGCAGCGGTGGCCGTGAGCACGccc > 4:80301/1‑149 (MQ=255) ttGCCCGCGAATACGATGGTGGATTCACCGCCGCATTCGCGGGCAAGCCCGCTCCCACAGTGTTCGGCGCAAGCCACGGGATCACTTGAATCGAGGTTTTGACATGAAAGttt > 5:111911/1‑113 (MQ=255) ttGCCCGCGAATACGATGGTGGATTCACCGCCGCATTCGCGGGCAAGCCCGCTCCCACAGTGTTCGGCGCAAGCCACGGGATCACTTGAATCGAGGTTTTGACATGAAAGttt < 6:111911/113‑1 (MQ=255) ccGCGAATGCGGCGGTGAATCCACCATCGTATTCGCGGGCAAGCCCGCTCCCACAGTGTTCGGCGCAAGCCACGGGATCACTTGAATCGAGGTTTTGACATGAAAGTTTTGATCATCGGCAGCGGTGGCCGTGAGCACGCcctggcctg > 6:409081/1‑149 (MQ=37) ccGCGAATGCGGCGGTGAATCCACCATCGTATTCGCGGGCAAGCCCGCTCCCACAGTGTTCGGCGCAAGCCACGGGATCACTTGAATCGAGGTTTTGACATGAAAGGTTTGATCATCGGCAGCGGTAGCCGTGAGCACGCcctggcctg > 4:76769/1‑149 (MQ=25) ccGCGAATACGATGGTGGATTCACCGCCGCATTCGCGGGCAAGCCCGCTCCCACAGTGTTCGGCGCAAGCCACGGGATCTCTTGAATCGAGGTTTTGACATGAAAGTTTTGATCATCGGCAGCGGTGGCCGTGAGCACGCcctggcctg > 4:232609/1‑149 (MQ=255) ccGCGAATACGATGGTGGATTCACCGCCGCATTCGCGGGCAAGCCCGCTCCCACAGTGTTCGGCGCAAGCCACGGGATCACTTGAATCGAGGTTTTGACATGAAAGTTTTGATCATCGGCAGCGGTGGCCGTGAGCACGCcctggcctg > 8:370374/1‑149 (MQ=255) ccGCGAATACGATGGTGGATTCACCGCCGCATTCGCGGGCAAGCCCGCTCCCACAGTGTTCGGCGCAAGCCACGGGATCACTTGAATCGAGGTTTTGACATGAAAGTTTTGATCATCGGCAGCGGTGGCCGTGAGCACGCcctggcctg > 6:92006/1‑149 (MQ=255) ccGCGAATACGATGGTGGATTCACCGCCGCATTCGCGGGCAAGCCCGCTCCCACAGTGTTCGGCGCAAGCCACGGGATCACTTGAATAGAGGTTTTGACATGAAAGTTTTGATCATCGGCAGCGGTGGCCGTGAGCACGCcctggcctg > 7:255683/1‑149 (MQ=255) gAATACGATGGTGGATTCACCGCCGCATTCGCGGGCAAGCCCGCTCCCACAGTGTTCGGCGCAAGCCACGGGATCACTTGAATCGAGGTTTTGACATGAAAGTTTTGATCATCGGCAGCGGTGGCCGTGAGCACGCCCTGGCCTGGaaa < 7:370374/149‑1 (MQ=255) gATGGTGGATTCACCGCCGCATTCGCGGGCAAGCCCGCTCCCACAGTGTTCGGCGCAAGCCACGGGATCACTTGAATCGAGGTTTTGACATGAAAGTTTTGATCATCGGCAGCGGTGGCCGTGAGCACGCCCTGGCCTGGAAAGTCGcc > 5:296438/1‑149 (MQ=255) gATGGTGGATTCACCGCCGCATTCGCGGGCAAGCCCGCTCCCACAGTGTTCGGCGCAAGCCACGGGATCACTTGAATCGAGGTTTTGACATGAAAGTTTTGATCATCGGCAGCGGTGGCCGTGAGCACGCCCTGGCCTGGAAAGTCGcc > 5:219697/1‑149 (MQ=255) gATGGTGGATTCACCGCCGCATTCGCGGGCAAGCCCGCTCCCACAGTGTTCGGCGCAAGCCACGGGATCACTTGAATCGAGGTTTTGACATGAAAGTTTTGATCATCGGCAGCGGTGGCCGTGAGCACGCCCTGGCCTGGAAAGTCGcc > 5:149334/1‑149 (MQ=255) gATGGTGGATTCACCGCCGCATTCGCGGGCAAGCCCGCTCCCACAGTGTTCGGCGCAAGCCACGGGATCACTTGAATCGAGGTTTTGACATGAAAGTTTTGATCATCGGCAGCGGTGGCCGTGAGCACGCCCTGGCCTGGAAAGTCGc > 7:246119/1‑148 (MQ=255) aTGGTGGATTCACCGCCGCATTCGCGGGCAAGCCCGCTCCCACAGTGTTCGGCGCAAGCCACGGGATCACTTGAATCGAGGTTTTGACATGAAAGTTTTGATCATCGGCAGCGGTGGCCGTGAGCACGCCCTGGCCTGGAAAGTCGcc > 2:286876/1‑148 (MQ=255) tggATTCACCGCCGCATTCGCGGGCAAGCCCGCTCCCACAGTGTTCGGCGCAAGCCACGGGATCACTTGAATCGAGGTTTTGACATGAAAGTTTTGATCATCGGCAGCGGTGGCCGTGAGCACGCcctggcct > 6:27662/1‑133 (MQ=255) tggATTCACCGCCGCATTCGCGGGCAAGCCCGCTCCCACAGTGTTCGGCGCAAGCCACGGGATCACTTGAATCGAGGTTTTGACATGAAAGTTTTGATCATCGGCAGCGGTGGCCGTGAGCACGCcctggcct < 5:27662/133‑1 (MQ=255) tggATTCACCGCCGCATTCGCGGGCAAGCCCGCTCCCACAGTGTTCGGCGCAAGCCACGGGATCACTTGAATCGAGGTTTTGACATGAAAGTTTTGATCATCGGCAGCGGTGGCCGTGAGCACGCCCTGGCCTGGAAAGTCGCCCAGGa < 6:296438/149‑1 (MQ=255) aCCGCCGCATTCGCGGGCAAGCCCGCTCCCACAGTGTTCGGCGCAAGCCACGGGATCACTTGAATCGAGGTTTTGACATGAAAGTTTTGATCATCGGCAGCGGTGGCCGTGAGCACGCCCTGGCCTGGAAAGTCGCCCAGGACCCAcgc > 7:151368/1‑149 (MQ=255) cgccgcATTCGCGGGCAAGCCCGCTCCCACAGTGTTCGGCGCAAGCCACGGGATCACTTGAATCGAGGTTTTGACATGAAAGTTTTGATCATCGGCAGCGGTGGCCGTGAGCACGCCCTGGCCTGGAAAGTCGCCCAGGAc > 5:43686/1‑141 (MQ=255) cgccgcATTCGCGGGCAAGCCCGCTCCCACAGTGTTCGGCGCAAGCCACGGGATCACTTGAATCGAGGTTTTGACATGAAAGTTTTGATCATCGGCAGCGGTGGCCGTGAGCACGCCCTGGCCTGGAAAGTCGCCCAGGAc < 6:43686/141‑1 (MQ=255) cgccgcATTCGCGGGCAAGCCCGCTCCCACAGTGTTCGGCGCAAGCCACGGGATCACTTGAATCGAGGTTTTGACATGAAAGTTTTGATCATCGGCAGCGGTGGCCGTGAGCACGCCCTGGCCTGGAAAGTCGCCCAGGACCCACGCGt < 5:92006/149‑1 (MQ=255) | TCGCTGCTGCCGACGAGGCCGGCATCGCGATGGTCTTCACTGGCATGCGCCACTTCCGCCACTAATTACGCGGATCCCGAGGCAAGTCGAGATC‑CTGTGGGAGCGGGCTTGCCCGCGAATACGATGGTGGATTCACCGCCGCATTCGCGGGCAAGCCCGCTCCCACAGTGTTCGGCGCAAGCCACGGGATCACTTGAATCGAGGTTTTGACATGAAAGTTTTGATCATCGGCAGCGGTGGCCGTGAGCACGCCCTGGCCTGGAAAGTCGCCCAGGACCCACGCGT > NC_002947/5485030‑5485314 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |