Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 431,362 C→T 5.9% V318I (GTT→ATT)  PP_0354 ← CBS domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947431,3620CT 5.9% 201.5 / 2.1 68V318I (GTT→ATT) PP_0354CBS domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base C (34/30);  new base T (2/2);  total (36/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.97e-01

GGGTCGCCACGTTCGGCCAGGGCCAGTTCGATGACCCGGCACACGGTATGGTCGTTGAGCAAGGTAATGATCTGGGTGATCTGCGTCGACGATGCGCCATGGGCCAGCATGCGTTCTACAAGCTGGCTGATTTCGCCGCGCAGCGAAACCAGCGTATCCAGGCGTGGCGCATGGCGTATGGTGCGCGCCAGGTGAACCAGATCGACCCGTTGCAGGGAGAACAGGTCGCGCTCGGAAACCACGCCGCACAGGCGTCCGTTTTCCACCAGGCAGACATGGGCAATATGCCGCTCGGT  >  NC_002947/431214‑431509
                                                                                                                                                    |                                                                                                                                                   
gggTCGCCACGTTCGGCCAGGGCCAGTTCGATGACCCGGCACACGGTATGGTCGTTGAGCAAGGTAATGATCTGGGTGATCTGCGTCGACGATGCGCCATGGGCCAGCATGCGTTCTACAAGCTGGCTGATTTCGCCGCGCAGCGAAAc                                                                                                                                                     <  6:104514/149‑1 (MQ=255)
     gCCACGTTCGGCCAGGGCCAGTTCGATGACCCGGCACACGGTATGGTCGTTGAGCAAGGTAATGATCTGGGTGATCTGCGTCGACGATGCGCCATGGGCCAGCATGCGTTCTACAAGCTGGCTGGTTTCGCTGCGCGGCGAAATCAGcc                                                                                                                                                <  2:46195/149‑2 (MQ=255)
     gCCACGTTCGGCCAGGGCCAGTTCGATGACCCGGCACACGGTATGGTCGTTGAGCAAGGTAATGATCTGGGTGATCTGCGTCGACGATGCGCCATGGGCCAGCATGCGTTCTACAAGCTGGCTGATTTCGCCGCGCAGCGAAACCa                                                                                                                                                   <  6:47812/146‑1 (MQ=255)
     gCCACGTTCGGCCAGGGCCAGTTCGATGACCCGGCACACGGTATGGTCGTTGAGCAAGGTAATGATCTGGGTGATCTGCGTCGACGATGCGCCATGGGCCAGCATGCGTTCTACAAGCTGGCTGATTTCGCCGCGCAGCGAAACCa                                                                                                                                                   >  5:47812/1‑146 (MQ=255)
     gCCACGTTCGGCCAGGGCCAGTTCGATGACCCGGCACACGGTATGGTCGTTGAGCAAGGTAATGATCTGGGTGATCTGCGTCGACGATGCGCCATGGGCCAGCATGCGTTCTACAAGCTGGCTGATTTCGCCGCGCAGCGAAACCAGc                                                                                                                                                 <  4:175079/148‑1 (MQ=255)
      ccACGTTCGGCCAGGGCCAGTTCGATGACCCGGCACACGGTATGGTCGTTGAGCAAGGTAATGATCTGGGTGATCTGCGTCGACGATGCGCCATGGGCCAGCATGCGTTCTACAAGCTGGCTGATTTCGCCGCGCAGCGAAACCAGCg                                                                                                                                                >  1:42779/1‑148 (MQ=255)
      ccACGTTCGGCCAGGGCCAGTTCGATGACCCGGCACACGGTATGGTCGTTGAGCAAGGTAATGATCTGGGTGATCTGCGTCGACGATGCGCCATGGGCCAGAATGCGTTCTACAAGCTGGCTGATTTCGCCGCGCAGCGAAACAAGCg                                                                                                                                                >  5:401471/1‑148 (MQ=255)
       cACGTTCGGCCAGGGCCAGTTCGATGACCCGGCACACGGTATGGTCGTTGAGCAAGGTAATGATCTGGGTGATCTGCGTCGACGATGCGCCATGGGCCAGCATGCGTTCTACAAGCTGGCTGGTTTCGCTGCGCGGCGAAATCAGc                                                                                                                                                 <  4:80637/146‑1 (MQ=255)
       cACGTTCGGCCAGGGCCAGTTCGATGACCCGGCACACGGTATGGTCGTTGAGCAAGGTAATGATCTGGGTGATCTGCGTCGACGATGCGCCATGGGCCAGCATGCGTTCTACAAGCTGGCTGGTTTCGCTGCGCGGCGAAATCAGc                                                                                                                                                 >  3:80637/1‑146 (MQ=255)
             cGGCCAGGGCCAGTTCGATGACCGGGCACACGGTATGGTCGTTGAGCAAGGTAATGATCTGGGTGATATGCGTCGACCATGCGACATGGGCCAGCATGCGTTCTAGAAGCTGGCTGATTTCGCCGCGCAGCGAAACCAGCGTATCCAg                                                                                                                                         <  6:86081/148‑1 (MQ=255)
               gccagggccagTTCGATGACCCGGCACACGGTATGGTCGTTGAGCAAGGTAATGATCTGGGTGATCTGCGTCGACGATGCGCCATGGGCCAGCATGCGTTCTACAAGCTGGCTGATTTCGCCGCGCAGCGAAACCAGCGTATCCAGGCg                                                                                                                                      >  1:217812/1‑149 (MQ=255)
                ccagggccagTTCGATGACCCGGCACACGGTATGGTCGTTGAGCAAGGTAATGATCTGGGTGATCTGCGTCGACGATGCGCCATGGGCCAGCATGCGTTCTACAAGCTGGCTGATTTCGCCGCGCAGCGAAACCAGCGTATCCAGGCGt                                                                                                                                     >  3:334941/1‑149 (MQ=255)
                ccagggccagTTCGATGACCCGGCACACGGTATGGTCGTTGAGCAAGGTAATGATCTGGGTGATCTGCGTCGACGATGCGCCATGGGCCAGCATGCGTTCTACAAGCTGGCTGATTTCGCCGCGCAGCGAAACCAGCGTATCCAGGCGt                                                                                                                                     >  3:58623/1‑149 (MQ=255)
                 cagggccagTTCGATGACCCGGCACACGGTATGGTCGTTGAGCAAGGTAATGATCTGGGTGATCTGCGTCGACGATGCGCCATGGGCCAGCATGCGTTCTACAAGCTGGCTGATTTCGCCGCGCAGCGAAACCAGc                                                                                                                                                 <  3:174004/136‑1 (MQ=255)
                 cagggccagTTCGATGACCCGGCACACGGTATGGTCGTTGAGCAAGGTAATGATCTGGGTGATCTGCGTCGACGATGCGCCATGGGCCAGCATGCGTTCTACAAGCTGGCTGATTTCGCCGCGCAGCGAAACCAGc                                                                                                                                                 >  4:174004/1‑136 (MQ=255)
                 cagggccagTTCGATGACCCGGCACACGGTATGGTCGTTGAGCAAGGTAATGATCTGGGTGATCTGCGTCGACGATGCGCCATGGGCCAGCATGCGTTCTACAAGCTGGCTGATTTCGCCGCGCAGCGAAACCAGCGTATCCAGGCGTg                                                                                                                                    >  6:402220/1‑149 (MQ=255)
                  aggtcaagTTCGATGACCCGGCACCCGGTATGGGCGTTGACCAAGGTAATGATCTGGGGGATCTGCGTCGACGATGCGCCATGGGCCAGCATGCGTTCTACAAGCTGGCTGATTTCGCCGCGCAGCGAAACCAGCGTATCCAGGCGTgg                                                                                                                                   <  8:366014/143‑1 (MQ=255)
                     gccagTTCGATGACCCGGCACACGGTATGGTCGTTGAGCAACGTAATGATCTGGGTGCTCTGCGTCGACGATGCGCCATGGGCCAGCATGCGTTCTACAAGCTGGCTGATTTCGCCGCGCAGCGAAACCAGCGTATCCAGGCGTGgcgc                                                                                                                                <  8:181183/149‑1 (MQ=255)
                      ccagTTCGATGACCCGGCACACGGTATGGTCGTTGAGCAAGGTAATGATCTGGGTGATCTGCGTCGACGATGCGCCATGGGCCAGCATGCGTTCTACAAGCTGGCTGATTTCGCCGCGCAGCGAAACCAGCGTATCCAGGCGTGGCGCa                                                                                                                               >  1:304198/1‑149 (MQ=255)
                      ccagTTCGATGACCCGGCACACGGTATGGTCGTTGAGCAAGGTAATGATCTGGGTGATCTGCGTCGACGATGCGCCATGGGCCAGCATGCGTTCTACAAGCTGGATGATTTCGCCGCGCAGCGAAACCAGCGTATCCAGGCGTGGCGCa                                                                                                                               >  6:42652/1‑149 (MQ=255)
                       cagTTCGATGACCCGGCACACGGTATGGTCGTTGAGCAAGGTAATGATCTGGGTGATCTGCGTCGACGATGCGCCATGGGCCAGCATGCGTTCTACAAGCTGGCTGATTTCGCCGCGCAGCGAAAc                                                                                                                                                     >  5:263275/1‑126 (MQ=255)
                       cagTTCGATGACCCGGCACACGGTATGGTCGTTGAGCAAGGTAATGATCTGGGTGATCTGCGTCGACGATGCGCCATGGGCCAGCATGCGTTCTACAAGCTGGCTGATTTCGCCGCGCAGCGAAAc                                                                                                                                                     <  6:263275/126‑1 (MQ=255)
                          ttCGATGACCCGGCACACGGTATGGTCGTTGAGCAAGGTAATGATCTGGGTGATCTGCGTCGACGATGCGCCATGGGCCAGCATGCGTTCTACAAGCTGGCTGATTTCGCCGCGCAGCGAAACCAGCGTATCCAGGCGTGGCGCATGGc                                                                                                                           >  1:144262/1‑149 (MQ=255)
                              aTGACCCGGCACACGGTATGGTCGTTGAGCAAGGTAATGATCTGGGTGATCTGCGTCGACGATGCGCCATGGGCCAGCATGCGTTCTACAAGCTGGCTGATTTCGCCGCGCAGCGAAACCAGCGTATCCAGGCGTGGCGCATGGCGTAt                                                                                                                       >  7:367788/1‑149 (MQ=255)
                                     ggCACACGGTATGGTCGTTGAGCAAGGTAATGATCTGGGTGATCTGCGTCGACGATGCGCCATGGGCCAGCATGCGTTCTACAAGCTGGCTGATTTCGCCGCGCAGCGAAACCa                                                                                                                                                   >  8:140609/1‑114 (MQ=255)
                                     ggCACACGGTATGGTCGTTGAGCAAGGTAATGATCTGGGTGATCTGCGTCGACGATGCGCCATGGGCCAGCATGCGTTCTACAAGCTGGCTGATTTCGCCGCGCAGCGAAACCa                                                                                                                                                   <  7:140609/114‑1 (MQ=255)
                                           cGGTATGGTCGTTGAGCAAGGTAATGATCTGGGTGATCTGCGTCGACGATGCGCCATGGGCCAGCATGCGTTCTACAAGCTGGCTGATTTCGCCGCGCAGCGAAACCAGCGTATCCAGGCGTGGCGCATGGCGTATGGTGCGCGCCAgg                                                                                                          <  2:42779/149‑1 (MQ=255)
                                            ggTATGGTCGTTGAGCAAGGTAATGATCTGGGTGATCTGCGTCGACGATGCGCCATGGGCCAGCATGCGTTCTACAAGCTGGCTGATTTCGCCGCGCAGCGAAACCAGCGTATCCAGGCGTGGCGCATGGCGTATGGTGCGCGCCAGGt                                                                                                         <  5:410597/149‑1 (MQ=255)
                                              tATGGTCGTTGAGCAAGGTAATGATCTGGGTGATCTGCGTCGACGATGCGCCATGGGCCAGCATGCGTTCTACAAGCTGGCTGATTTCGCCGCGCAGCGAAACCAGCGTATCCAGGCGTGGCGCATGGCGTATGGTGCGCGCCAGGTg                                                                                                        >  7:262914/1‑148 (MQ=255)
                                                  gTCGTTGAGCAAGGTAATGATCTGGGTGATCTGCGTCGACGATGCGCCATGGGCCAGCATGCGTTCTACAAGCTGGCTGATTTCGCCGCGCAGCGAAACCAGCGTATCCAGGCGTGGCGCATGGCGTATGGTGCGCGCCAGGTGAAcc                                                                                                    <  8:262914/148‑1 (MQ=255)
                                                     gTTGAGCAAGGTAATGATCTGGGTGATCTGCGTCGACGATGCGCCATGGGCCAGCATGCGTTCTACAAGCTGGCTGATTTCGCCGCGCAGCGAAACCAGCGTATCCAGGCGTGGCGCATGGCGTATGGTGCGCGCCAGGTGAACCAGa                                                                                                 <  1:261435/148‑1 (MQ=255)
                                                      gtcaGCAAGGTAATGATCTGGGTGCTCTGCGTCGACGATGCGCCATGGGCCAGCATGCGGTCTACAAGCTGGCTGATTTCGCCGCGCAGCGAAACCAGCGTATCCAGGCGTGGCGCATGGCTTATGGTGCGCGCCAGGTGAACCAGATc                                                                                               <  3:50621/146‑1 (MQ=255)
                                                         aGCAAGGTAATGATCTGGGTGATCTGCGTCGACGATGCGCCATGGGCCAGCATGCGTTCTACAAGCTGGCTGATTTCGCCGCGCAGCGAAACCAGCGTATCCAGGCGTGGCGCATGGCGTATGGTGCGCGCCAGGTGAACCAGATCGAc                                                                                            <  3:52460/149‑1 (MQ=255)
                                                          gCAAGGTAATGATCTGGGTGATCTGCGTCGACGATGCGCCATGGGCCAGCATGCGTTCTACAAGCTGGCTGATTTCGCCGCGCAGCGAAACCAGCGTATCCAGGCGTGGCGCATGGCGTATGGTGCGCGCCAGGTGAACCAGATCGAc                                                                                            <  4:334941/148‑1 (MQ=255)
                                                              ggTAATGATCTGGGTGATCTGCGTCGACGATGCGCCATGGGCCAGCATGCGTTCTACAAGCTGGCTGATTTCGCCGCGCAGCGAAACCAGCGTATCCAGGCGTGGCGCATGGCGTATGGTGCGCGCCAGGTGAACCAGATCGACCCGtt                                                                                       <  8:367788/149‑1 (MQ=255)
                                                                tAATGATCTGGGTGATCTGCGTCGACGATGCGCCATGGGCCAGCATGCGTTCTACAAGCTGGCTGATTTCGCCGCGCAGCGAAACCAGCGTATCCAGGCGTGGCGCATGGCGTATGGTGCGCGCCAGGTGAACCAGATCGACCCGTTGc                                                                                     <  5:351606/149‑1 (MQ=255)
                                                                  aTGATCTGGGTGATCTGCGTCGACGATGCGCCATGGGCCAGCATGCGTTCTACAAGCTGGCTGATTTCGCCGCGCAGCGAAACCAGCGTATCCAGGCGTGGCGCATGGCGTATGGTGCGCGCCAGGTGAACCAGATCGACCCGTTGCAg                                                                                   >  1:312118/1‑149 (MQ=255)
                                                                  aTGATCTGGGTGATCTGCGTCGACGATGCGCCATGGGCCAGCATGCGTTCTACAAGCTGGCTGATTTCGCCGCGCAGCGAAACCAGCGTATCCAGGCGTGGCGCATGGCGTATGGTGCGCGCCAGGTGAACCAGATCGACCCGTTGCAg                                                                                   >  5:57661/1‑149 (MQ=255)
                                                                      tCTGGGTGATGTGCGTCGACGATGCGCCATGGGCCAGCATGCGTTCTACAAGCTGGCTGATTTCGCCGCGCAGCGAAACCAGCGTATCCAGGCGTGGCGCATGGCGTATGGTGCGCGCCAGGTGAACCAGATCGACCCGTTGCAGGga                                                                                <  4:255256/148‑1 (MQ=255)
                                                                          ggTGATCTGCGTCGACGATGCGCCATGGGCCAGCATGCGTTCTACAAGCTGGCTGATTTCGCCGCGCAGCGAAACCAGCGTATCCAGGCGTGGCGCATGGCGTATGGTGCGCGCCAGGTGAACCAGATCGACCCGTTGCAGGga                                                                                <  2:217812/144‑1 (MQ=255)
                                                                           gTGATCTGCGTCGACGATGCGCCATGGGCCAGCATGCGTTCTACAAGCTGGCTGATTTCGCCGCGCAGCGAAACCAGCGTATCCAGGCGTGGCGCATGGCGTATGGTGCGCGCCAGGTGAACCAGATCGACCCGTTGCAGGGAGaa                                                                             >  3:336693/1‑146 (MQ=255)
                                                                           gTGATCTGCGTCGACGATGCGCCATGGGCCAGCATGCGTTCTACAAGCTGGCTGATTTCGCCGCGCAGCGAAACCAGCGTATCCAGGCGTGGCGCATGGCGTATGGTGCGCGCCAGGTGAACCAGATCGACCCGTTGCAGGGAGAACa                                                                           <  4:336693/148‑1 (MQ=255)
                                                                             gATCTGCGTCGACGATGCGCCATGGGCCAGCATGCGTTCTACAAGCTGGCTGATTTCGCCGCGCAGCGAAACCAGCGTATCCAGGCGTGGCGCATGGCGTATGGTGCGCGCCAGGTGAACCAGATCGACCCGTTGCAGGGAGAACAGGt                                                                        >  2:180262/1‑149 (MQ=255)
                                                                                   cGTCGACGATGCGCCATGGGCCAGCATGCGTTCTACAAGCTGGCTGATTTCGCCGCGCAGCGAAACCAGCGTATCCAGGCGTGGCGCATGGCGTATGGTGCGCGCCAGGTGAACCAGATCGACCCGTTGCAGGGAGAACAGGTCgcgc                                                                   <  6:57661/148‑1 (MQ=255)
                                                                                     tCGACGATGCGCCATGGGCCAGCATGCGTTCTACAAGCTGGCTGATTTCGCCGCGCAGCGAAACCAGCGTATCCAGGCGTGGCGCATGGCGTATGGTGCGCGCCAGGTGAAc                                                                                                     <  7:282641/112‑1 (MQ=255)
                                                                                     tCGACGATGCGCCATGGGCCAGCATGCGTTCTACAAGCTGGCTGATTTCGCCGCGCAGCGAAACCAGCGTATCCAGGCGTGGCGCATGGCGTATGGTGCGCGCCAGGTGAAc                                                                                                     >  8:282641/1‑112 (MQ=255)
                                                                                       gacgaTGCGCCATGGGCCAGCATGCGTTCTACAAGCTGGCTGATTTCGCCGCGCAGCGAAACCAGCGTATCCAGGCGTGGCGCATGGCGTATGGTGCGCGCCAGGTGAACCAGATCGACCCGTTGCAGGGAGAACAGGTCGCGCTCgg                                                               <  5:402220/148‑1 (MQ=255)
                                                                                         cgaTGCGCCATGGGCCAGCATGCGTTCTACAAGCTGGCTGATTTCGCCGCGCAGCGAAACCAGCGTATCCAGGCGTGGCGCATGGCGTATGGTGCGCGCCAGGTGAACCAGATCGa                                                                                             >  6:391256/1‑116 (MQ=255)
                                                                                         cgaTGCGCCATGGGCCAGCATGCGTTCTACAAGCTGGCTGATTTCGCCGCGCAGCGAAACCAGCGTATCCAGGCGTGGCGCATGGCGTATGGTGCGCGCCAGGTGAACCAGATCGa                                                                                             <  5:391256/116‑1 (MQ=255)
                                                                                             gcgcCATGGGCCAGCATGCGTTCTACAAGCTGGCTGATTTCGCCGCGCAGCGAAACCAGCGTATCCAGGCGTGGCGCATGGCGTATGGTGCGCGCCAGGTGAACCAGATCGACCCGTTGCAGGGAGAACAGGTCGCGCTCGGAAACCAc                                                        >  6:413378/1‑149 (MQ=255)
                                                                                              cgcCATGGGCCAGCATGCGTTCTACAAGCTGGCTGATTTCGCCGCGCAGCGAAACCAGCGTATCCAGGCGTGGCGCATGGCGTATGGTGCGCGCCAGGTGAACCAGATCGACCCGTTGCAGGGAGAACAGGTCGCGCTCGGAAACCAcg                                                       >  7:288200/1‑149 (MQ=255)
                                                                                               gcCATGGGCCAGCATGCGTTCTACTAGCTGGCTGATTTCGCCTGGCAGCGAAACGAGCGTATCCAGGCGTGGCGCATGGCGTATGGTGCGCGCCAGGTGAACCAGATCGACCCGTTGCAGGGAGAACAGGTCGCGCTCGGAAACCAcgc                                                      <  6:401471/149‑1 (MQ=255)
                                                                                               gcCATGGGCCAGCATGCGTTCTACAAGCTGGCTGATTTCGCCGCGCAGCGAAACCAGCGTATCCAGGCGTGGCGCATGGCGTATGGTGCGCGCCAGGTGAACCAGATCGACCCGTTGCAGGGAGAACAGGTCGCGCTCGGAACCCAcgc                                                      >  3:302476/1‑149 (MQ=255)
                                                                                                           cATGCGTTCTACAAGCTGGCTGATTTCGCCGCGCAGCGAAACCAGCGTATCCAGGCGTGGCGCATGGCGTATGGTGCGCGCCAGGTGAACCAGATCGACCCGTTGCAGGGAGAACAGGTCGCGCTCGGAAACCACGCCGCACAGGCGTc                                          >  6:251564/1‑149 (MQ=255)
                                                                                                               cGTTCTACAAGCTGGCTGATTTCGCCGCGCAGCGAAACCAGCGTATCCAGGCGTGGCGCATGGCGTATGGTGCGCGCCAGGTGAACCAGATCGACCCGTTGCAGGGAGAACAGGTCGCGCTCGGAAACCACGCCGCACAGGCGTCCGtt                                      >  4:223407/1‑149 (MQ=255)
                                                                                                                gTTCTACAAGCTGGCTGATTTCGCCGCGCAGCGAAACCAGCGTATCCAGGCGTGGCGCATGGCGTATGGTGCGCGCCAGGTGAACCAGATCGACCCGTTGCAGGGAGAACAGGTCGCGCTCGGAAACCACGCCGCACAGGCGTCCGttt                                     >  1:389149/1‑149 (MQ=255)
                                                                                                                gTTCTACAAGCTGGATGATTTCGCCGCGCAGCGAAACCAGCGTATCCAGGCGTGGCGCATGGCGTATGGTGCGCGCCAGGTGAACCAGATCGACCCGTTGCAGGGAGAACAGGTCGCGCTCGGAAACCACGCCGCACAGGCGTCCGttt                                     <  5:42652/149‑1 (MQ=255)
                                                                                                                 ttCTACAAGCTGGCTGATTTCGCCGCGCAGCGAAACCAGCGTATCCAGGCGTGGCGCATGGCGTATGGTGCGCGCCAGGTGAACCAGATCGACCCGTTGCAGGGAGAACAGGTCGCGCTCGGAAACCACGCCGCACAGGCGTCCGttt                                     >  2:5468/1‑148 (MQ=255)
                                                                                                                    tACAAGCTGGCTGATTTCGCCGCGCAGCGAAACCAGCGTATCCAGGCGTGGCGCATGGCGTATGGTGCGCGCCAGGTGAACCAGATCGACCCGTTGCAGGGAGAACAGGTCGCGCTCGGAAACCACGCCGCACAGGCGTCCGTTTTcca                                 >  5:329428/1‑149 (MQ=255)
                                                                                                                      cAAGCTGGCTGATTTCGCCGCGCAGCGAAACCAGCGTATCCAGGCGTGGCGCATGGCGTATGGTGCGCGCAAGGTGAACCAGATCGACCCGTTGCAGGGAGAACAGGTCGCGCTCGGAAACCACGCCGCACAGGCGTCCGTTTTccacc                               <  2:312118/149‑1 (MQ=255)
                                                                                                                             gctgGTTTCGCTGCGCGGCGAAATCAGCGTATCCAGGCGTGGCGCATGGCGTATGGTGCGCGCCAGGTGAACCAGATCGACCCGTTGCAGGGAGAACAGGTCGCGCTCGGAAACCACGCCGCACAGGCGTCCGTTTTCCACCAGGCAGa                        >  3:257436/1‑149 (MQ=255)
                                                                                                                              ctgATTTCGCCGCGCAGCGAAACCAGCGTATCCAGGCGTGGCGCATGGCGTATGGTGCGCGCCAGGTGAACCAGATCGACCCGTTGCAGGGAGAACAGGTCGCGCTCGGAAACCACGCCGCACAGGCGTCCGTTTTCCACCAGGCAGa                        >  4:327505/1‑148 (MQ=255)
                                                                                                                                gATTTCGCCGCGCAGCGAAACCAGCGTATCCAGGCGTGGCGCATGGCGTATGGTGCGCGCCAGGTGAACCAGATCGACCCGTTGCAGGGAGAACAGGTCGCGCTCGGAAACCACGCCGCACAGGCGTCCGTTTTCCACCAGGCAGACAt                     >  2:236239/1‑149 (MQ=255)
                                                                                                                                 aTTTCGCCGCGCAGCGAAACCAGCGTATCCAGGCGTGGCGCATGGCGTATGGTGCGCGCCAGGTGAACCAGATCGACCCGTTGCAGGGAGAACAGGTCGCGCTCGGAAACCACGCCGCACAGGCGTCCGTTTTCCACCAGGCAGACATg                    <  4:302476/149‑1 (MQ=255)
                                                                                                                                   ttCGCCGCGCAGCGAAACCAGCGTATCCAGGCGTGGCGCATGGCGTATGGTGCGCGCCAGGTGAACCAGATCGACCCGTTGCAGGGAGAACAGGTCGCGCTCGGAAACCACGCCGCACAGGCGTCCGTTTTCCACCAGGCAGACATgg                   <  2:144262/148‑1 (MQ=255)
                                                                                                                                      gccgcGCAGCGAAACCAGCGTATCCAGGCGTGGCGCATGGCGTATGGTGCGCGCCAGGTGAACCAGATCGACCCGTTGCAGGGAGAACAGGTCGCGCTCGGAAACCACGCCGCACAGGCGTCCGTTTTc                                   >  4:305285/1‑129 (MQ=255)
                                                                                                                                      gccgcGCAGCGAAACCAGCGTATCCAGGCGTGGCGCATGGCGTATGGTGCGCGCCAGGTGAACCAGATCGACCCGTTGCAGGGAGAACAGGTCGCGCTCGGAAACCACGCCGCACAGGCGTCCGTTTTc                                   <  3:305285/129‑1 (MQ=255)
                                                                                                                                         gcgcAGCGAAACCAGCGTATCCAGGCGTGGCGCATGGCGTATGGTGCGCGCCAGGTGAACCAGATCGACCCGTTGCAGGGAGAACAGGTCGCGCTCGGAAACCACGCCGCACAGGCGTCCGTTTTCCACCAGGCAGACATGGGCAatat            >  2:290756/1‑149 (MQ=255)
                                                                                                                                                 aaaCCAGCGTATCCAGGCGTGGCGCATGGCGTATGGTGCGCGCCAGGTGAACCAGCTCGACCCGTTGCAGGGAGAACAGGTCGCGCTCGGAAACCACGCCGCACAGGCGTCCGTTTTCCACCAGGCAGACATGGGCAATATGCCGCTCg    >  8:187160/1‑149 (MQ=255)
                                                                                                                                                 aaaCCAGCGTATCCAGGCGTGGCGCATGGCGTATGGTGCGCGCCAGGTGAACCAGATCGACCCGTTGCAGGGAGAACAGGTCGCGCTCGGAACCCACGCCGCACAGGCGTCCGTTTTCCACCAGGCAGACATGGGCAATATGCCGCTCg    >  8:166280/1‑149 (MQ=255)
                                                                                                                                                   aCCAGCGTATCCAGGCGTGGCGCATGGCGTATGGTGCGCGCCAGGTGAACCAGATCGACCCGTTGCAGGGAGAACAGGTCGCGCTCGGAAACCACGCCGCACAGGCGTCCGTTTTCCACCAGGCAGACATGGGCAATATGCCGCTCGGt  <  8:288200/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GGGTCGCCACGTTCGGCCAGGGCCAGTTCGATGACCCGGCACACGGTATGGTCGTTGAGCAAGGTAATGATCTGGGTGATCTGCGTCGACGATGCGCCATGGGCCAGCATGCGTTCTACAAGCTGGCTGATTTCGCCGCGCAGCGAAACCAGCGTATCCAGGCGTGGCGCATGGCGTATGGTGCGCGCCAGGTGAACCAGATCGACCCGTTGCAGGGAGAACAGGTCGCGCTCGGAAACCACGCCGCACAGGCGTCCGTTTTCCACCAGGCAGACATGGGCAATATGCCGCTCGGT  >  NC_002947/431214‑431509

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑