Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,515,926	G→C	5.7%	P34A (CCC→GCC)	cbpA ←	curved DNA‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,515,926	0	G	C	5.7%	225.4 / 3.5	70	P34A (CCC→GCC)	cbpA	curved DNA‑binding protein
Reads supporting (aligned to +/- strand):  ref base G (28/38);  new base C (2/2);  total (30/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.79e-01

TGGTGCCTGGAACGGCCGGCCATGCTGGCCGCCGTACTTGCGGATTTCATCGAACTCGGCACGTTTCTGC‑GCGTC‑‑GCCCAGCACCTCATAGGCCTCGTTGGCCTCCTTGAATTTCTCCTCGGCGTCGCGCTCCTTACTGACATCGGGGTG‑ATACTTGCGCGCCAGTTTGCGGTACGCGGCCTTGATCGCCTTTTCGTC‑CGCAGTGGGCTCTACGCCGAGTATCTTGTAATAGTCTTTGAAGTCCATTTATGGATCACCAATGTGAATGTGCATGTTGCAACTGAAGATAGGGGT  >  NC_002947/5515780‑5516073                                                                                                                                                      |                                                                                                                                                      tggtgCCTGGAACGGCCGGCCATGCTGGCCGCCGTACTTGCGGATTTCATCGAACTCGGCACGTTTCTGC‑GCGTC‑‑GCCCAGCACCTCATAGGCCTCGTTGGCCTCCTTGAATTTCTCCTCGGCGTCGCGCTCCTTACTGACATCGGGGt                                                                                                                                                     <  3:41509/149‑1 (MQ=255) tggtgCCTGGAACGGCCGGCCATGCTGGCCGCCGTACTTGCGGATTTCATCGAACTCGGCACGTTTCTGC‑GCGTC‑‑GCCCAGCACCTCATAGGCCTCGTTGGCCTCCTTGAATTTCTCCTCGGCGTCGCGCTCCTTACTGACATCGGGGt                                                                                                                                                     <  7:360475/149‑1 (MQ=255) tggtgCCTGGAACGGCCGGCCATGCTGGCCGCCGTACTTGCGGATTTCATCGAACTCGGCACGTTTCTGC‑GCGTC‑‑GCCCAGCACCTCATAGGCCTCGTTGGCCTCCTTGAATTTCTCCTCGGCGTCGCGCTCCTTACTGACATCGGGGt                                                                                                                                                     <  4:171329/149‑1 (MQ=255)   gtgCCTGGAAAGGCCGGGCATGCTGGCCGCCGTACTTGCGGATTTCATCGAACTCGGCACGTTTGTTCGGGGTCCTCCCCAGCACCTCATAGGCCTCGTTGGCCTCCTTGAATTTCTCCTCGGCGTCGCGCTCCTTACTGACATCggg                                                                                                                                                       <  1:330509/148‑1 (MQ=255)      ccTGGAACGGCCGGCCATGCTGGCCGCCGTACTTGCGGATTTCATCGAACTCGGCACGTTTCTGC‑GCGTC‑‑GCCCAGCACCTCATAGGCCTCGTTGGCCTCCTTGAATTTCTCCTCGGCGTCGCGCTCCTTACTGACATCGGGGTG‑ATAc                                                                                                                                               >  4:358485/1‑149 (MQ=255)          gAACGGCCGGCCATGCTGGCCGCCGTACTTGCGGATTTCATCGAACTCGGCACGTTTCTGC‑GCGTC‑‑GCCCAGCACCTCATAGGCCTCGTTGGCCTCCTTGAATTTCTCCTCGGCGTCGCGCTCCTTACTGACATCGGGGTG‑ATACtt                                                                                                                                             <  4:14113/147‑1 (MQ=255)            aCGGCCGGCCATGCTGGCCGCCGTACTTGCGGATTTCATCGAACTCGGCACGTTTCTGC‑GCGTC‑‑GCCCAGCACCTCATAGGCCTCGTTGGCCTCCTTGAATTTCTCCTCGGCGTCGCGCTCCTTACTGACATCGGGGTG‑ATACTTgcgc                                                                                                                                         <  1:355667/149‑1 (MQ=255)            aCGGCCGGCCATGCTGGCCGCCGTACTTGCGGATTTCATCGAACTCGGCACGTTTCTGC‑GCGTC‑‑GCCCAGCACCTCATAGGCCTCGTTGGCCTCCTTGAATTTCTCCTCGGCGTCGCGCTCCTTACTGACATCGGGGTG‑ATACTTgcgc                                                                                                                                         <  5:23078/149‑1 (MQ=255)                ccggccATGCTGGCCGCCGTACTTGCGGATTTCATCGAACTCGGCACGTTTCTGC‑GCGTC‑‑GCCCAGCACCTCATAGGCCTCGTTGGCCTCCTTGAATTTCTCCTCGGCGTCGCGCTCCTTACTGACATCGGGGTG‑ATACTTgcgcgc                                                                                                                                       >  4:331039/1‑147 (MQ=255)                ccggccATGCTGGCCGCCGTACTTGCGGATTTCATCGAACTCGGCACGTTTCTGC‑GCGTC‑‑GCCCAGCACCTCATAGGCCTCGTTGGCCTCCTTGAATTTCTCCTCGGCGTCGCGCTCCTTACTGACATCGGGGTG‑ATACTTgcgcgc                                                                                                                                       >  5:207073/1‑147 (MQ=255)                      aTGCTGGCCGCCGTACTTGCGGATTTCATCGAACTCGGCACGTTTCTGC‑GCGTC‑‑GCCCAGCACCTCATAGGCCTCGTTGGCCTCCTTGAATTTCTCCTCGGCGTCGCGCTCCTTACTGACATCGGGGTG‑ATACTTGCGCGCCAGTTTGc                                                                                                                               <  6:207073/149‑1 (MQ=255)                       tGCTGGCCGCCGTACTTGCGGATTTCATCGAACTCGGCACGTTTCTGC‑GCGTC‑‑GCCCAGCACCTCATAGGCCTCGTTGGCCTCCTTGAATTTCTCCTCGGCGTCGCGCTCCTTACTGACATCGGGGTG‑ATACTTGCGCGCCAGTTTGCg                                                                                                                              <  3:331039/149‑1 (MQ=255)                       tGCTGGCCGCCGTACTTGCGGATTTCATCGAACTCGGCACGTTTCTGC‑GCGTC‑‑GCCCAGCACCTCATAGGCCTCGTTGGCCTCCTTGAATTTCTCCTCGGCGTCGCGCTCCTTACTGACATCGGGGTG‑ATACTTGCGCGCCAGTTTGCg                                                                                                                              >  3:68455/1‑149 (MQ=255)                          tGGCCGCCGTACTTGCGGATTTCATCGAACTCGGCACGTTTCTGC‑GCGTC‑‑GCCCAGCACCTCATAGGCCTCGTTGGCCTCCTTGAATTTCTCCTCGGCGTCGCGCTCCTTACTGACATCGGGGTG‑ATACTTGCGCGCCAGTTTGCGGTa                                                                                                                           >  3:42615/1‑149 (MQ=255)                            gccgccGTACTTGCGGATTTCATCGAACTCGGCACGTTTCTGC‑GCGTC‑‑GCCCAGCACCTCATAGGCCTCGTTGGCCTCCTTGAATTTCTCCTCGGCGTCGCGCTCCTTACTGACATCGGGGTG‑ATACTTGCGCGCCAGTTTGCGGTAcg                                                                                                                         >  6:199196/1‑149 (MQ=255)                               gccgTACTTGCGGATTTCATCGAACTCGGCACGTTTCTGC‑GCGTC‑‑GCCCAGCACCTCATAGGCCTCGTTGGCCTCCTTGAATTTCTCCTCGGCGTCGCGCTCCTTACTGACATCGGGGTG‑ATACTTGCGCGCCAGTTTGCGGTACGCgg                                                                                                                      <  7:101370/149‑1 (MQ=255)                                ccgTACTTGCGGATTTCATCGAACTCGGCACGTTTCTGC‑GCGTC‑‑GCCCAGCACCTCATAGGCCTCGTTGGCCTCCTTGAATTTCTCCTCGGCGTCGCGCTCCTTACTGACATCGGGGTG‑ATACTTGCGCGCCAGTTTGCGGTACGCGGc                                                                                                                     >  3:354477/1‑149 (MQ=255)                                ccgTACTTGCGGATTTCATCGAACTCGGCACGTTTCTGC‑GCGTC‑‑GCCCAGCACCTCATAGGCCTCGTTGGCCTCCTTGAATTTCTCCTCGGCGTCGCGCTCCTTACTGACATCGGGGTG‑ATACTTGCGCGCCAGTTTGCGGTACGCGGc                                                                                                                     <  7:223774/149‑1 (MQ=255)                                  gTACTTGCGGATTTCATCGAACTCGGCACGTTTCTGC‑GCGTC‑‑GCCCAGCACCTCATAGGCCTCGTTGGCCTCCTTGAATTTCTCCTCGGCGTCGCGCTCCTTACTGACATCGGGGTG‑ATACTTGCGCGCCAGTTTGCGGTACGCGGCCt                                                                                                                   <  3:358485/149‑1 (MQ=255)                                   tACTTGCGGATTTCATCGAACTCGGCACGTTTCTGC‑GCGTC‑‑GCCCAGCACCTCATAGGCCTCGTTGGCCTCCTTGAATTTCTCCTCGGCGTCGCGCTCCTTACTGACATCGGGGTG‑ATACTTCCGCGCCAGTTTGCGGTACGCGGCCtt                                                                                                                  <  1:12297/149‑1 (MQ=255)                                      ttGCGGATTTCATCGAACTCGGCACGTTTCTGC‑GCGTC‑‑GCCCAGCACCTCATAGGCCTCGTTGGCCTCCTTGAATTTCTCCTCGGCGTCGCGCTCCTTACTGACATCGGGGTG‑ATACTTGCGCGCCAGTTTGCGGTACGCGGCCTTGAt                                                                                                               >  1:53195/1‑149 (MQ=255)                                        gCGGATTTCATCGAACTCGGCACGTTTCTGC‑GCGTC‑‑GCCCAGCACCTCATAGGCCTCGTTGGCCTCCTTGAATTTCTCCTCGGCGTCGCGCTCCTTACTGACATCGGGGTG‑ATACTTGCGCGCCAGTTTGCGGTACGCGGCCTTGATc                                                                                                              <  8:222892/148‑1 (MQ=255)                                          ggATTTCATCGAACTCGGCACGTTTCTGC‑GCGTC‑‑GCCCAGCACCTCATAGGCCTCGTTGGCCTCCTTGAATTTCTCCTCGGCGTCGCGCTCCTTACTGACATCGGGGTG‑ATACTTGCGCGCCAGTTTGCGGTACGCGGCCTTGATCGcc                                                                                                           >  2:220469/1‑149 (MQ=255)                                           gATTTCATCGAACTCGGCACGTTTCTGC‑GCGTC‑‑GCCCAGCACCTCATAGGCCTCGTTGGCCTCCTTGAATTTCTCCTCGGCGTCGCGCTCCTTACTGACATCGGGGTG‑ATACTTGCGCGCCAGTTTGCGGTACGCGGCCTTGATCGCCt                                                                                                          >  1:300341/1‑149 (MQ=255)                                            aTTTCATCGAACTCGGCACGTTTCTGC‑GCGTC‑‑GCCCAGCACCTCATAGGCCTCGTTGGCCTCCTTGAATTTCTCCTCGGCGTCGCGCTCCTTACTGACATCGGGGTG‑ATACTTGCGCGCCAGTTTGCGGTACGCGGCCTTGATCGCCtt                                                                                                         <  3:113029/149‑1 (MQ=255)                                            aTTTCATCGAACTCGGCACGTTTCTGC‑GCGTC‑‑GCCCAGCACCTCATAGGCCTCGTTGGCCTCCTTGAATTTCTCCTCGGCGTCGCGCTCCTTACTGACATCGGGGTG‑ATACTTGCGCGCCAGTTTGCGGTACGCGGCCTTGATCGCCtt                                                                                                         <  3:149515/149‑1 (MQ=255)                                               tCATCGAACTCGGCACGTTTCTGC‑GCGTC‑‑GCCCAGCACCTCATAGGCCTCGTTGGCCTCCTTGAATTTCTCCTCGGCGTCGCGCTCCTTACTGACATCGGGGTG‑ATACTTGCGCGCCAGTTTGCGGTACGCGGCCTTGATCGCCtttt                                                                                                       <  4:330508/148‑1 (MQ=255)                                                cATCGAACTCGGCACGTTTCTGC‑GCGTC‑‑GCCCAGCACCTCATAGGCCTCGTTGGCCTCCTTGAATTTCTCCTCGGCGTCGCGCTCCTTACTGACATCGGGGTG‑ATACTTGCGCGCCAGTTTGCGGTACGCGGCCTTGATCGCCTTTTc                                                                                                      <  2:229291/148‑1 (MQ=255)                                                cATCGAACTCGGCACGTTTCTGC‑GCGTC‑‑GCCCAGCACCTCATAGGCCTCGTTGGCCTCCTTGAATTTCTCCTCGGCGTCGCGCTCCTTACTGACATCGGGGTG‑ATACTTGCGCGCCAGTTTGCGGTACGCGGCCTTGATCGCCTTTTCg                                                                                                     >  3:356503/1‑149 (MQ=255)                                                 aTCGAACTCGGCACGTTTCTGC‑GCGTC‑‑GCCCAGCACCTCATAGGCCTCGTTGGCCTCCTTGAATTTCTCCTCGGCGTCGCGCTCCTTACTGACATCGGGGTG‑ATACTTGCGCGCCAGTTTGCGGTACGCGGCCTTGATCGCCTTTTCGt                                                                                                    >  4:365340/1‑149 (MQ=255)                                                  tCGAACTCGGCACGTTTCTGC‑GCGTC‑‑GCCCAGCACCTCATAGGCCTCGTTGGCCTCCTTGAATTTCTCCTCGGCGTCGCGCTCCTTACTGACATCGGGGTG‑ATACTTGCGCGCCAGTTTGCGGTACGCGGCCTTGATCGCCTTTTCGTc                                                                                                   <  4:354477/149‑1 (MQ=255)                                                    gAACTCGGCACGTTTCTGC‑GCGTC‑‑GCCCAGCACCTCATAGGCCTCGTTGGCCTCCTTGAATTTCTCCTCGGCGTCGCGCTCCTTACTGACATCGGGGTGCATACTTGCGCGCCAGTTTGCGGTACGCGGCCTTGATCGCCTTTTCGTc‑c                                                                                                 <  1:95154/149‑1 (MQ=255)                                                    gAACTCGGCACGTTTCTGC‑GCGTC‑‑GCCCAGCACCTCATAGGCCTCGTTGGCCTCCTTGAATTTCTCCTCGGCGTCGCGCTCCTTACTGACATCGGGGTG‑ATACTTGCGCGCCAGTTTGCGGTACGCGGCCTTGATCGCCTTTTCGTc‑c                                                                                                 <  3:334022/148‑1 (MQ=255)                                                    gAACTCGGCACGTTTCTGC‑GCGTC‑‑GCCCAGCACCTCATAGGCCTCGTTGGCCTCCTTGAATTTCTCCTCGGCGTCGCGCTCCTTACTGACATCGGGGTG‑ATACTTGCGCGCCAGTTTGCGGTACGCGGCCTTGATCGCCTTTTCGTC‑Cg                                                                                                <  1:220469/149‑1 (MQ=255)                                                          ggCACGTTTCTGC‑GCGTC‑‑GCCCAGCACCTCATAGGCCTCGTTGGCCTCCTTGAATTTCTCCTCGGCGTCGCGCTCCTTACTGACATCGGGGTG‑ATACTTGCGCGCCAGTTTGCGGTACGCGGCCTTGATCGCCTTTTCGTC‑CGCAGTgg                                                                                          >  6:368651/1‑149 (MQ=255)                                                           gCACGTTTCTGC‑GCGTC‑‑GCCCAGCACCTCATAGGCCTCGTTGGCCTCCTTGAATTTCTCCTCGGCGTCGCGCTCCTTACTGACATCGGGGTG‑ATACTTGCGCGCCAGTTTGCGGTACGCGGCCTTGATCGCCTTTTc                                                                                                      >  8:353810/1‑137 (MQ=255)                                                           gCACGTTTCTGC‑GCGTC‑‑GCCCAGCACCTCATAGGCCTCGTTGGCCTCCTTGAATTTCTCCTCGGCGTCGCGCTCCTTACTGACATCGGGGTG‑ATACTTGCGCGCCAGTTTGCGGTACGCGGCCTTGATCGCCTTTTc                                                                                                      <  7:353810/137‑1 (MQ=255)                                                                tttCTGC‑GCGTC‑‑GCCCAGCACCTCATAGGCCTCGTTGGCCTCCTTGAATTTCTCCTCGGCGTCGCGCTCCTTACTGACATCGGGGTG‑ATACTTGCGCGCCAGTTTGCGGTACGCGGCCTTGATCGCCTTTTCGTC‑CGCAGTGGGCTCTa                                                                                    <  4:42615/149‑1 (MQ=255)                                                                      c‑gcgTC‑‑GCCCAGCACCTCATAGGCCTCGTTGGCCTCCTTGAATTTCTCCTCGGCGTCGCGCTCCTTACTGACATCGGGGTG‑ATACTTGCGCGCCAGTTTGCGGTACGCGGCCTTGATCGCCTTTTCGTC‑CGCAGTGGGCTCTACGc                                                                                 <  8:32480/146‑1 (MQ=255)                                                                      c‑gcgTC‑‑GCCCAGCACCTCATAGGCCTCGTTGGCCTCCTTGAATTTCTCCTCGGCGTCGCGCTCCTTACTGACATCGGGGTG‑ATACTTGCGCGCCAGTTTGCGGTACGCGGCCTTGATCGCCTTTTCGTC‑CGCAGTGGGCTCTACGCCGa                                                                              <  5:199196/149‑1 (MQ=255)                                                                          gTC‑‑GCCCAGCACCTCATAGGCCTCGTTGGCCTCCTTGAATTTCTCCTCGGCGTCGCGCTCCTTACTGACATCGGGGTG‑ATACTTg                                                                                                                                            >  8:96370/1‑85 (MQ=255)                                                                          gTC‑‑GCCCAGCACCTCATAGGCCTCGTTGGCCTCCTTGAATTTCTCCTCGGCGTCGCGCTCCTTACTGACATCGGGGTG‑ATACTTg                                                                                                                                            <  7:96370/85‑1 (MQ=255)                                                                            c‑‑GCCCAGCACCTCATAGGCCTCGTTGGCCTCCTTGAATTTCTCCTCGGCGTCGCGCTCCTTACTGACATGGGGGTG‑ATACTTGCGCGCCAGTTTGCGGTACGCGGCCTTGATCGCCTTTTCGTC‑CGCAGTTGGCTCTACGCCGAGTATc                                                                         <  1:250049/149‑1 (MQ=255)                                                                               gCCCAGCACCTCATAGGCCTCGTTGGCCTCCTTGAATTTCTCCTCGGCGTCGCGCTCCTTACTGACATCGGGGTG‑ATACTTGCGCGCCAGTTTGCGGTACGCGGCCTTGATCGCCTTTTCGTC‑CGCAGTGGGCTCTACGCCGAGTATc                                                                         <  2:300341/148‑1 (MQ=255)                                                                               gCCCAGCACCTCATAGGCCTCGTTGGCCTCCTTGAATTTCTCCTCGGCGTCGCGCTCCTTACTGACATCGGGGTG‑ATACTTGCGCGCCAGTTTGCGGTACGCGGCCTTGATCGCCTTTTCGTC‑CGCAGTGGGCTCTACGCCGAGTATCt                                                                        >  2:80208/1‑149 (MQ=255)                                                                                 ccAGCACCACAAAGGCCACGTTGGCCTCCTTGAATTTCTCCTCGGCGTCGCGCTCCTTACTGACATCGGGGTG‑ATACTTGAGCGCCAGTTTGCGGTACGCGGCCTTGATCGCCTTTTCGTC‑CGCAGTGGGCTCTACGCCGAGTaacatg                                                                      >  8:309854/1‑144 (MQ=255)                                                                                  cAGCACCTCATAGGCCTCGTTGGCCTCCTTGAATTTCTCCTCGGCGTCGCGCTCCTTACTGACATCGGGGTG‑ATACTTGCGCGCCAGTTTGCGGTACGCGGCCTTGATCGCCTTTTCGTC‑CGCAGTGGGCTCTACGCCGAGTATCTTg                                                                      >  2:304177/1‑148 (MQ=255)                                                                                        cTCATAGGCCTCGTTGGCCTCCTTGAATTTCTCCTCGGCGTCGCGCTCCTTACTGACATCGGGGTG‑ATACTTGCGCGCCAGTTTg                                                                                                                                >  5:163734/1‑85 (MQ=255)                                                                                        cTCATAGGCCTCGTTGGCCTCCTTGAATTTCTCCTCGGCGTCGCGCTCCTTACTGACATCGGGGTG‑ATACTTGCGCGCCAGTTTg                                                                                                                                <  6:163734/85‑1 (MQ=255)                                                                                        cTCATAGGCCTCGTTGGCCTCCTTGAATTTCTCCTCGGCGTCGCGCTCCTTACTGACATCGGGGTG‑ATACTTGCGCGCCAGTTTGCGGTACGCGGCCTTGATCGCCCTTTCGTC‑CGCAGTGGGCTCTACGCCGAGTATCTTGTAATAGt                                                               <  4:68455/149‑1 (MQ=255)                                                                                              ggCCTCGTTGGCCTCCTTGAATTTCTCCTCGGCGTCGCGCTCCTTACTGACATCGGGGTG‑ATACTTGCGCGCCAGTTTGCGGTACGCGGCCTTGATCGCCTTTTCGTC‑CGCAGTGGGCTCTACGCCGAGTATCTTGTAATAGTCTTTg                                                          <  6:218183/148‑1 (MQ=255)                                                                                               gCCTCGTTGGCCTCCTTGAATTTCTCCTCGGCGTCGCGCTCCTTACTGACATCGGGGTG‑ATACTTGCGCGCCAGTTTGCGGTACGCGGCCTTGATCGCCTTTTCGTC‑CGCAGTGGGCTCTACGCCGAGTATCTTGTAATAGTCTTTg                                                          >  2:346587/1‑147 (MQ=255)                                                                                                ccTCGTTGGCCTCCTTGAATTTCTCCTCGGCGTCGCGCTCCTTACTGACATCGGGGTG‑ATACTTGCGCGCCAGTTTGCGGTACGCGGCCTTGATCGCCTTTTCGTC‑CGCAGTGGGCTCTACGCCGAGTATCTTGTAATAGTCTTTGAAg                                                       >  7:223528/1‑149 (MQ=255)                                                                                                        gCCTCCTTGAATTTCTCCTCGGCGTCGCGCTCCTTACTGACATCGGGGTG‑ATACTTGCGCGCCAGTTTGCGGTACGCGGCCTTGATCGCCTTTTc                                                                                                      <  3:174364/95‑1 (MQ=255)                                                                                                        gCCTCCTTGAATTTCTCCTCGGCGTCGCGCTCCTTACTGACATCGGGGTG‑ATACTTGCGCGCCAGTTTGCGGTACGCGGCCTTGATCGCCTTTTc                                                                                                      >  4:174364/1‑95 (MQ=255)                                                                                                          ctcctTGAATTTCTCCTCGGCGTCGCGCTCCTTACTGACATCGGGGTG‑ATACTTGCGCGCCAGTTTGCGGTACGCGGCCTTGATCGCCTTTTCGTCTCGCAGTGGGCTCTACGCCGAGTATCTTGTAATAGTCTTTGAAGTCCATTTAt                                              <  1:304177/149‑1 (MQ=255)                                                                                                             ctTGAATTTCTCCTCGGCGTCGCGCTCCTTACTGACATCGGGGTG‑ATACTTGCGCGCCAGTTTGCGGTACGCGGCCTTGATCGCCTTTTCGTC‑CGCAGTGGGCTCTACGCCGAGTATCTTGTAATAGTCTTTGAAGTCCATTTATgg                                            <  8:286308/147‑1 (MQ=255)                                                                                                             ctTGAATTTCTCCTCGGCGTCGCGCTCCTTACTGACATCGGGGTG‑ATACTTGCGCGCCAGTTTGCGGTACGCGGCCTTGATCGCCTTTTCGTC‑CGCAGTGGGCTCTACGCCGAGTATCTTGTAATAGTCTTTGAAGTCCATTTATgg                                            <  1:62810/147‑1 (MQ=255)                                                                                                             ctTGAATTTCTCCTCGGCGTCGCGCTCCTTACTGACATCGGCGTG‑ATACTTGCGCGCCAGTTTGCGGTACGCGGCCTTGATCGCCTTTTCGTC‑CGCAGTGGGCTCTACGCCGAGTATCTTGTAATAGTCTTTGAAGTCCATTTATGGAt                                          >  6:186667/1‑149 (MQ=255)                                                                                                             ctTGAATTTCTCCTCGGCGTCGCGCTCCTTACTGACATCGGCGTG‑ATACTTGCGCGCCAGTTTGCGGTACGCGGCCTTGATCGCCTTTTCGTC‑CGCAGTGGGCTCTACGCCGAGTATCTTGTAATAGTCTTTGAAGTCCATTTATGGAt                                          >  6:180655/1‑149 (MQ=255)                                                                                                                          tcGGCGTCGCGCTCCTTACTGACATCGGGGTG‑ATACTTGCGCGCCAGTTTGCGGTACGCGGCCTTGATCGCCTTTTCGTC‑CGCAGTGGGCTCTACGCCGAGTATCTTGTAATAGTCTTTGAAGTCCATTTATGGATCACCaatgtgaat                             >  2:351625/1‑149 (MQ=255)                                                                                                                            ggCGTCGCGCTCCTTACTGACATCGGGGTG‑ATACTTGCGCGCCAGTTTGCGGTACGCGGCCTTGATCGCCTTTTCGTC‑CGCAGTGGGCTCTACGCCGAGTATCTTGTAATAGTCTTTGAAGTCCATTTATGGATCACCaatgtgaatgt                           <  1:80208/149‑1 (MQ=255)                                                                                                                            ggCGTCGCGCTCCTTACTGACATCGGGGTG‑ATACTTGCGCGCCAGTTTGCGGTACGCGGCCTTGATCGCCTTTTCGTC‑CGCAGTGGGCTCTACGCCGAGTATCTTGTAATAGTCTTTGAAGTCCATTTATGGATCACCaatgtgaat                             <  3:365340/147‑1 (MQ=255)                                                                                                                            ggCGTCGCGCTCCTTACTGACATCGGCGTG‑ATACTTGCGCGCCAGTTTGCGGTACGCGGCCTTGATCGCCTTTTCGTC‑CGCAGTGGGCTCTACGCCGAGTATCTTGTAATAGTCTTTGAAGTCCATTTATGGATCACCaatgtgaat                             <  5:180655/147‑1 (MQ=255)                                                                                                                            ggCGTCGCGCTCCTTACTGACATCGGCGTG‑ATACTTGCGCGCCAGTTTGCGGTACGCGGCCTTGATCGCCTTTTCGTC‑CGCAGTGGGCTCTACGCCGAGTATCTTGTAATAGTCTTTGAAGTCCATTTATGGATCACCaatgtgaat                             <  5:186667/147‑1 (MQ=255)                                                                                                                                   cgcTCCTTACTGACATCGGGGTG‑ATACTTGCGCGCCAGTTTGCGGTACGCGGCCTTGATCGCCTTTTCGTC‑CGCAGTGGGCTCTACGCCGAGTATCTTGTAATAGTCTTTGAAGTCCATTTATGGATCACCAATGTGAATGTGCATGtt                    >  7:267786/1‑149 (MQ=255)                                                                                                                                     cTCCTTACTGACATCGGGGTG‑ATACTTGCGCGCCAGTTTGCGGTACGCGGCCTTGATCGCCTTTTCGTC‑CGCAGTGGGCTCTACGCCGAGTATCTTGTAATAGTCTTTGAAGTCCATTTATGGATCACCAATGTGAATGTGCATGTTGc                  <  4:356503/149‑1 (MQ=255)                                                                                                                                      tCCTTACTGACATCGGGGTG‑ATACTTGCGCGCCAGTTTGCGGTACGCGGCCTTGATCGCCTTTTCGTC‑CGCAGTGGGCTCTACGCCGAGTATCTTGTAATAGTCTTTGAAGTCCATTTATGGATCACCAATGTGAATGTGCATGTTGc                  >  1:63995/1‑148 (MQ=255)                                                                                                                                            cTGACATCGGGGTG‑ATACTTGCGCGCCAGTTTGCGGTACGCGGCCTTGATCGCCTTTTCGTC‑CGCAGTGGGCTCTACGCCGAGTATCTTGTAATAGTCTTTGAAGTCCATTTATGGATCACCAATGTGAATGTGCATGTTGCAACTGaa           >  7:218716/1‑149 (MQ=255)                                                                                                                                               aCATCGGGGTG‑ATACTTGCGCGCCAGTTTGCGGTACGCGGCCTTGATCGCCTTTTCGTC‑CGCAGTGGGCTCTACGCCGAGTATCTTGTAATAGTCTTTGAAGTCCATTTATGGATCACCAATGTGAATGTGCATGTTGCAACTGAAGa         >  6:279363/1‑148 (MQ=255)                                                                                                                                                  tCGGGGTG‑ATACTTGCGCGCCAGTTTGCGGTACGCGGCCTTGATCGCCTTTTCGTC‑CGCAGTGGGCTCTACGCCGAGTATCTTGTAATAGTCTTTGAAGTCCATTTATGGATCACCAATGTGAATGTGCATGTTGCAACTGAAGATAgg     >  3:165161/1‑149 (MQ=255)                                                                                                                                                    ggggTG‑ATACTTGCGCGCCAGTTTGCGGTACGCGGCCTTGATCGCCTTTTCGTC‑CGCAGTGGGCTCTACGCCGAGTATCTTGTAATAGTCTTTGAAGTCCATTTATGGATCACCAATGTGAATGTGCATGTTGCAACTGAAGATAgggg   >  4:33351/1‑149 (MQ=255)                                                                                                                                                    ggggTG‑ATACTTGCGCGCCAGTTTGCGGTACGCGGCCTTGATCGCCTTTTCGTC‑CGCAGTGGGCTCTACGCCGAGTATCTTGTAATAGTCTTTGAAGTCCATTTATGGATCACCAATGTGAATGTGCATGTTGCAACTGAAGATAggg    <  1:351625/148‑1 (MQ=255)                                                                                                                                                    ggggTG‑ATACTTGCGCGCCAGTTTGCGGTACGCGGCCTTGATCGCCTTTTCGTC‑CGCAGTGGGCTCTACGCCGAGTATCTTGTAATAGTCTTTGAAGTCCATTTATGGATCACCAATGTGAATGTGCATGTTGCAACTGAAGATAggg    <  8:223528/148‑1 (MQ=255)                                                                                                                                                    ggggTG‑ATACTTGCGCGCCAGTTTGCGGTACGCGGCCTTGATCGCCTTTTCGTC‑CGCAGTGGGCTCTACGCCGAGTATCTTGTAATAGTCTTTGAAGTCCATTTATGGATCACCAATGTGAATGTGCATGTTGCAACTGAAGATAggg    <  2:19106/148‑1 (MQ=255)                                                                                                                                                     gggTG‑ATACTTGCGCGCCAGTTTGCGGTACGCGGCCTTGATCGCCTTTTCGTC‑CGCAGTGGGCTCTACGCCGAGTATCTTGTAATAGTCTTTGAAGTCCATTTATGGATCACCAATGTGAATGTGCATGTTGCAACTGAAGATAGGGGt  >  2:403836/1‑149 (MQ=255)                                                                                                                                                      |                                                                                                                                                      TGGTGCCTGGAACGGCCGGCCATGCTGGCCGCCGTACTTGCGGATTTCATCGAACTCGGCACGTTTCTGC‑GCGTC‑‑GCCCAGCACCTCATAGGCCTCGTTGGCCTCCTTGAATTTCTCCTCGGCGTCGCGCTCCTTACTGACATCGGGGTG‑ATACTTGCGCGCCAGTTTGCGGTACGCGGCCTTGATCGCCTTTTCGTC‑CGCAGTGGGCTCTACGCCGAGTATCTTGTAATAGTCTTTGAAGTCCATTTATGGATCACCAATGTGAATGTGCATGTTGCAACTGAAGATAGGGGT  >  NC_002947/5515780‑5516073

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑