Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,570,177 T→G 7.7% A24A (GCA→GCC) ‡ nnrD ← ADP‑dependent (S)‑NAD(P)H‑hydrate dehydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,570,1770TG 7.7% 178.0 / 4.2 62A24A (GCA→GCC) ‡nnrDADP‑dependent (S)‑NAD(P)H‑hydrate dehydratase
Reads supporting (aligned to +/- strand):  ref base T (27/30);  new base G (2/3);  total (29/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTGCTCGGGCCGGGTTGCCACGCTGACCAGCCCGGCGCCGCAACGCAGGGCGGCTTCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCG‑CCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAA‑CCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATTATACGCACCTGAGCCCGTATTGCCTTGCCCATCCATGTCCGCCTC  >  NC_002947/5570030‑5570324
                                                                                                                                                    |                                                                                                                                                    
gTGCTCGGGCCGGGTTGCCACGCTGACCAGCCCGGCGCCGCAACGCAGGGCGGCTTCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCG‑CCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCtgccgtg                                                                                                                                                     >  7:121260/1‑149 (MQ=255)
   cTCGGGCCGGGTTGCCACGCTGACCAGCCCGGCGCCGCAACGCAGGGCGGCTTCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCtgccgtgcc                                                                                                                                                   >  7:50119/1‑149 (MQ=255)
         ccGGGTTGCCACGCTGACCAGCCCGGCGCCGCAACGCAGGGCGGCTTCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCG‑CCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGc                                                                                                                                            >  7:259049/1‑149 (MQ=255)
         ccGGGTTGCCACGCTGACCAGCCCGGCGCCGCAACGCAGGGCGGCTTCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCG‑CCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGGCACGGCAGGc                                                                                                                                            <  1:373257/149‑1 (MQ=255)
                  cACGCTGACCAGCCCGGCGCCGCAACGCAGGGCGGCTTCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCG‑CCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCgg                                                                                                                                                 >  4:349982/1‑135 (MQ=255)
                  cACGCTGACCAGCCCGGCGCCGCAACGCAGGGCGGCTTCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCG‑CCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCgg                                                                                                                                                 <  3:349982/135‑1 (MQ=255)
                      cTGACCAGCCCGGCGCCGCAACGCAGGGCGGCTTCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCG‑CCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAA‑CCGTCAc                                                                                                                              <  6:350412/149‑1 (MQ=255)
                             gCCCGGCGCCGCAACGCAGGGCGGCTTCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCG‑CCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTCCCGTGCCGGCAGGCGGGCAA‑CCGTCACGctgctg                                                                                                                       >  7:135418/1‑149 (MQ=255)
                             gCCCGGCGCCGCAACGCAGGGCGGCTTCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCG‑CCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCAGCCGTGCCGGCAGGCGGGCAA‑CCGTCACgctgct                                                                                                                        >  2:393956/1‑148 (MQ=255)
                              cccGGCGCCGCAACGCAGGGCGGCTTCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCG‑CCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAA‑CCGTCACgctgct                                                                                                                        >  3:338727/1‑147 (MQ=255)
                                       gcAACGCAGGGCGGCTTCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCG‑CCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAA‑CCGTCACGCTGCTGAGCAATtgtg                                                                                                             <  8:108369/149‑1 (MQ=255)
                                       gcAACGCAGGGCGGCTTCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCG‑CCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAA‑CCGTCACGCTGCCGAGCAATtgtg                                                                                                             >  7:130885/1‑149 (MQ=255)
                                        cAACGCAGGGCGGCTTCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCG‑CCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAA‑CCGTCACGCTGCTGAGCAATTGTgg                                                                                                            <  8:121260/149‑1 (MQ=255)
                                           cGCAGGGCGGCTTCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAA‑CCGTCACGCTGCTGAGCAATTGTgggg                                                                                                          <  8:50119/149‑1 (MQ=255)
                                                    gcTTCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCG‑CCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAA‑CCGTCACGCTGCTGAGCAATTGTGGGGCATTACTggg                                                                                                <  1:149334/149‑1 (MQ=255)
                                                    gcTTCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCG‑CCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAA‑CCGTCACGCTGCTGAGCAATTGTGGGGCATTACTggg                                                                                                >  7:305297/1‑149 (MQ=255)
                                                    gcTTCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCG‑CCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAA‑CCGTCACGCTGCCGAGCAATTGTGGGGCATTACTggg                                                                                                <  8:130885/149‑1 (MQ=255)
                                                     cTTCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCG‑CCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCAGCCGTGCCGGCAGGCGGGCAA‑CCGTCACGCTGCTGAGCAATTGTGGGGCATTACTgggg                                                                                               <  1:393956/149‑1 (MQ=255)
                                                      ttCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCG‑CCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAA‑CCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGgt                                                                                              >  7:94013/1‑149 (MQ=255)
                                                           cACTGAGCAACACAGCGCCTCCGGTACCCAGGTCG‑CCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCgg                                                                                                                                          <  5:48200/101‑1 (MQ=255)
                                                           cACTGAGCAACACAGCGCCTCCGGTACCCAGGTCG‑CCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCgg                                                                                                                                          >  6:48200/1‑101 (MQ=255)
                                                                  cAACACAGCGCCTCCGGTACCCAGGTCG‑CCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAA‑CCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCg                                                                                  >  5:395412/1‑149 (MQ=255)
                                                                   aaCACAGCGCCTCCGGTACCCAGGTCG‑CCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAA‑CCGTCACGCTGCTGAGCAATTGTGGGGCATTACTggg                                                                                                >  4:315093/1‑134 (MQ=255)
                                                                   aaCACAGCGCCTCCGGTACCCAGGTCG‑CCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAA‑CCGTCACGCTGCTGAGCAATTGTGGGGCATTACTggg                                                                                                <  3:315093/134‑1 (MQ=255)
                                                                     cacaGCGCCTCCGGTACCCAGGTCG‑CCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAA‑CCGTCACgctg                                                                                                                          >  4:94394/1‑106 (MQ=255)
                                                                     cacaGCGCCTCCGGTACCCAGGTCG‑CCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAA‑CCGTCACgctg                                                                                                                          <  3:94394/106‑1 (MQ=255)
                                                                     cacaGCGCCTCCGGTACCCAGGTCG‑CCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAA‑CCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCa                                                                               >  1:379089/1‑149 (MQ=255)
                                                                     cacaGCGCCTCCGGTACCCAGGTCG‑CCCCCGACCACCAGTACATGGCCAAAGTCGCCTATGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAA‑CCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCa                                                                               >  7:344563/1‑149 (MQ=255)
                                                                        aGCGCCTCCGGTACCCAGGTCG‑CCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAA‑CCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATgg                                                                            <  8:88782/149‑1 (MQ=255)
                                                                        aGCGCCTCCGGTACCCAGGTCG‑CCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAA‑CCGTCACGCTGCTGAGCAATTGTGGGGAATTACTGGGGTGTTTGGTCTGCGGCATgg                                                                            >  8:2188/1‑149 (MQ=255)
                                                                                cGGTACCCAGGTCG‑CCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAA‑CCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATggg                                                                           <  5:336311/142‑1 (MQ=255)
                                                                                cGGTACCCAGGTCG‑CCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAA‑CCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATggg                                                                           >  6:336311/1‑142 (MQ=255)
                                                                                cGGTACCCAGGTCG‑CCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAA‑CCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGAGGTCaaa                                                                     <  1:402923/148‑1 (MQ=255)
                                                                                      ccAGGTCG‑CCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAA‑CCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGc                                                                  >  6:122018/1‑145 (MQ=255)
                                                                                      ccAGGTCG‑CCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAA‑CCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGc                                                                  <  5:122018/145‑1 (MQ=255)
                                                                                      ccAGGTCG‑CCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAA‑CCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTcc                                                               >  6:376878/1‑148 (MQ=255)
                                                                                             g‑CCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAA‑CCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCt                                                        <  8:94013/148‑1 (MQ=255)
                                                                                             g‑CCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAA‑CCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCt                                                        <  3:65129/148‑1 (MQ=255)
                                                                                                  ccGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAA‑CCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTcc                                                               <  3:2646/137‑1 (MQ=255)
                                                                                                  ccGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAA‑CCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTcc                                                               >  4:2646/1‑137 (MQ=255)
                                                                                                    gACCACCAGTACATGGCGAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAA‑CCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGaa                                                 <  6:395412/149‑1 (MQ=255)
                                                                                                        accaGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAA‑CCGTCACGCTGCTGAGCAATTGTGGGGAATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATtat                                             <  7:2188/149‑1 (MQ=255)
                                                                                                         ccaGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAA‑CCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATtata                                            <  4:338727/149‑1 (MQ=255)
                                                                                                         ccaGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAA‑CCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATtata                                            >  6:9107/1‑149 (MQ=255)
                                                                                                         ccaGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAA‑CCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATtata                                            >  3:287811/1‑149 (MQ=255)
                                                                                                          caGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAA‑CCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATTATAc                                           >  3:208801/1‑149 (MQ=255)
                                                                                                                   gCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAA‑CCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATtatagatcggg                                     >  7:13788/1‑139 (MQ=255)
                                                                                                                   gCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAA‑CCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATta                                              <  8:13788/137‑1 (MQ=255)
                                                                                                                     cAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAA‑CCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCg                                                                                  >  6:135648/1‑99 (MQ=255)
                                                                                                                     cAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAA‑CCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCg                                                                                  <  5:135648/99‑1 (MQ=255)
                                                                                                                            gCCTTTGTGCGCCTGCGCCTGCCGGGCCGGCTTGCTGGCAA‑CCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTCTTTCGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATTATACGCACCTCAGCCCGTAttg                         <  6:248429/149‑1 (MQ=255)
                                                                                                                                   tgGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAA‑CCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATTATACGCACCTGAGCCCGTAttgccttgc                   <  8:259049/148‑1 (MQ=255)
                                                                                                                                   tgGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAA‑CCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATTATACGCACCTGAGCCCGTAtt                          <  8:214603/141‑1 (MQ=255)
                                                                                                                                   tgGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAA‑CCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATTATACGCACCTGAGCCCGTAtt                          >  7:214603/1‑141 (MQ=255)
                                                                                                                                      gCATGCGCCTGCCGTGCCGGCAGGCGGGCAA‑CCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATTATACGCACCTGAGCCCGTATTGCCTTGCccat               <  5:376878/149‑1 (MQ=255)
                                                                                                                                        aTGCGCCTGCCGTGCCGGCAGGCGGGCAA‑CCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGTCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATTATACGCACCTGAGCCCGTATTGCCTTGCccatcc             <  4:208801/149‑1 (MQ=255)
                                                                                                                                        aTGCGCCTGCCGTGCCGGCAGGCGGGCAA‑CCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATTATACGCACCTGAGCCCGTATTGCCTTGCccatcc             <  5:9107/149‑1 (MQ=255)
                                                                                                                                        aTGCGCCTGCCGTGCCGGCAGGCGGGCAA‑CCGGCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAACGGCTCCAATGTCTGGCAGAATTATACGCACCTGAGCCCGTATTGCCTTGCccatcc             <  5:87410/149‑1 (MQ=255)
                                                                                                                                          gcgcCTGCCGTGCCGGCAGGCGGGCAATCCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCAGGGGGTCAAAGGCTCCAATGTCTGGCAGAATTATACGCACCTGAGCCCGTATTGCCTTGCccatcca            >  1:218379/1‑149 (MQ=255)
                                                                                                                                          gcgcCTGCCGTGCCGGCAGGCGGGCAA‑CCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAg                                                   <  6:91070/109‑1 (MQ=255)
                                                                                                                                          gcgcCTGCCGTGCCGGCAGGCGGGCAA‑CCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAg                                                   >  5:91070/1‑109 (MQ=255)
                                                                                                                                          ccgcCTGCCGGCACGGCAGGCGGGCAA‑CCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATTATACGCACCTGAGCCCGTATTGCCTTGCccatccat           <  4:325886/148‑1 (MQ=255)
                                                                                                                                          ccgcCTGCCGGCACGGCAGGCGGGCAA‑CCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATTATACGCACCTGAGCCCGTATTGCCTTGCccatccat           >  3:325886/2‑149 (MQ=255)
                                                                                                                                           cgcCTGCCGGCACGGCAGGCGGGCAA‑CCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATTATACGCACCTGAGCCCGTATTGCCTTGCCCATCCATg          >  3:4860/1‑149 (MQ=255)
                                                                                                                                                 ccgtgccgGCAGGCGGGCAA‑CCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATTATACGCACCTGAGCCCGTATTGCCTTGCCCATCCATGTCCGcc    <  2:379089/149‑1 (MQ=255)
                                                                                                                                                   gtgccgGCAGGCGGGCAA‑CCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATTATACGCACCTGAGCCCGTATTGCCTTGCCCATCCATGTCCGCctc  >  4:27227/1‑149 (MQ=255)
                                                                                                                                                    tgccgGCAGGCGGGCAA‑CCGTCACGCTGCTGCGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATTATACGCACCTGAGCCCGTATTGCCTTGCCCATCCATGTCCGCctc  >  4:129019/1‑148 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GTGCTCGGGCCGGGTTGCCACGCTGACCAGCCCGGCGCCGCAACGCAGGGCGGCTTCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCG‑CCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAA‑CCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATTATACGCACCTGAGCCCGTATTGCCTTGCCCATCCATGTCCGCCTC  >  NC_002947/5570030‑5570324

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑