Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,582,813 T→A 6.5% H178L (CAC→CTC)  PP_4911 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,582,8130TA 6.5% 255.3 / 5.4 88H178L (CAC→CTC) PP_4911hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (47/35);  new base A (4/2);  total (51/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.37e-01

ACCTGAGTCGGTGCAGCGGGCTTTTTCGGTGCCTCTTCGGCAATCGCCGGCTGGTTGGCCAGCGGCTTCACCGCCGTGCTCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGCTGGTTCTTGCCAGAATCCAGGCGTGTGATCGACAACAGCACTTGCACGTCCGGGCTG  >  NC_002947/5582666‑5582956
                                                                                                                                                   |                                                                                                                                               
aCCTGAGTCGGTGCAGCGGGCTTTTTCGGTGCCTCTTCGGCAATCGCCGGCTGGTTGGCCAGCGGCTTCACCGCCGTGCTCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGtg                                                                                                                                                >  2:409091/1‑149 (MQ=255)
  cTGAGTCGGTGCAGCGGGCTTTTTCGGTGCCTCTTCGGCAATCGCCGGCTGGTTGGCCAGCGGCTTCACCGCCGTGCTCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGtgct                                                                                                                                              >  7:224204/1‑149 (MQ=255)
     aGTCGGTGCAGCGGGCTTTTTCGGTGCCTCTTCGGCAATCGCCGGCTGGTTGGCCAGCGGCTTCACCGCCGTGCTCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGtgctg                                                                                                                                             <  5:401994/147‑1 (MQ=255)
          gTGCAGCGGGCTTTTTCGGTGCCTCTTCGGCAATCGCCGGCTGGTTGGCCAGCGGCTTCACCGCCGTGCTCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTc                                                                                                                                       >  1:83651/1‑148 (MQ=255)
             cAGCGGGCTTTTTCGGTGCCTCTTCGGCAATCGCCGGCTGGTTGGCCAGCGGCTTCACCGCCGTGCTCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAgg                                                                                                                                   <  5:391813/149‑1 (MQ=255)
             cAGCGGGCTTTTTCGGTGCCTCTTCGGCAATCGCCGGCTGGTTGGCCAGCGGCTTCACCGCCGTGCTCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAg                                                                                                                                    <  6:141136/148‑1 (MQ=255)
                cGGGCTTTTTCGGTGCCTCTTCGGCAATCGCCGGCTGGTTGGCCAGCGGCTTCACCGCCGTGCTCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGtg                                                                                                                                                <  6:68506/133‑1 (MQ=255)
                cGGGCTTTTTCGGTGCCTCTTCGGCAATCGCCGGCTGGTTGGCCAGCGGCTTCACCGCCGTGCTCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGtg                                                                                                                                                >  5:68506/1‑133 (MQ=255)
                cGGGCTTTTTCGGTGCCTCTTCGGCAATCGCCGGCTGGTTGGCCAGCGGCTTCACCGCCGTGCTCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTgg                                                                                                                                <  5:399522/149‑1 (MQ=255)
                cGGGCTTTTTCGGTGCCTCTTCGGCAATCGCCGGCTGGTTGGCCAGCGGCTTCACCGCCGTGCTCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTg                                                                                                                                 >  7:333337/1‑148 (MQ=255)
                   gCTTTTTCGGTGCCTCTTCGGCAATCGCCGGCTGGTTGGCCAGCGGCTTCACCGCCGTGCTCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGgat                                                                                                                              >  6:71778/1‑148 (MQ=255)
                    cTTTTTCGGTGCCTCTTCGGCATTGGCCGGGTGGTTGGCCAGCGGCTTCACCGCCGTGCTCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGgatga                                                                                                                            <  8:333337/149‑1 (MQ=255)
                     tttttCGGTGCCTCTTCGGCAATCGCCGGCTGGTTGGCCAGCGGCTTCACCGCCGTGCTCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGCCTCACTGCCCTCGTGCTGCTGCTCGAGGTgactac                                                                                                                            >  5:234203/1‑143 (MQ=255)
                     tttttCGGTGCCTCTTCGGCAATCGCCGGCTGGTTGGCCAGCGGCTTCACCGCCGTGCTCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGgatga                                                                                                                            >  3:214417/1‑148 (MQ=255)
                     tttttCGGTGCCTCTTCGGCAATCGCCGGCTGGTTGGCCAGCGGCTTCACCGCCGTGCTCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGgatga                                                                                                                            >  6:416682/1‑148 (MQ=255)
                       tttCGGTGCCTCTTCGGCAATCGCCGGCTGGTTGGCCAGCGGCTTCACCGCCGTGCTCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATa                                                                                                                          <  1:409091/148‑1 (MQ=255)
                          cGGTGCCTCTTCGGCAATCGCCGGCTGATTGGCCAGCGGCTTCACCGCCGTGCTCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGt                                                                                                                      >  1:133284/1‑149 (MQ=255)
                           ggTGCCTCTTCGGCAATCGCCGGCTGGTTGGCCAGCGGCTTCACCGCCGTGCTCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGtt                                                                                                                     <  2:40281/149‑1 (MQ=255)
                           ggTGCCTCTTCGGCAATCGCCGGCTGGTTGGCCAGCGGCTTCACCGCCGTGCTCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGtt                                                                                                                     <  1:273620/149‑1 (MQ=255)
                            gTGCCTCTTCGGCAATCGCCGGCTGGTTGGCCAGCGGCTTCACCGCCGTGCTCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATCCGGTTg                                                                                                                    >  2:141625/1‑149 (MQ=255)
                            gTGCCTCTTCGGCAATCGCCGGCTGGTTGGCCAGCGGCTTCACCGCCGTGCTCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTg                                                                                                                    >  7:292641/1‑149 (MQ=255)
                            gTGCCTCTTCGGCAATCGCCGGCTGGTTGGCCAGCGGCTTCACCGCCGTGCTCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGAGCAGCAGCACGAgg                                                                                                                                   <  5:137707/134‑1 (MQ=255)
                            gTGCCTCTTCGGCAATCGCCGGCTGGTTGGCCAGCGGCTTCACCGCCGTGCTCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGAGCAGCAGCACGAgg                                                                                                                                   >  6:137707/1‑134 (MQ=255)
                                ctctTCGGCAATCGCCGGCTGGTTGGCCAGCGGCTTCACCGCCGTGCTCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCAc                                                                                                                <  1:244189/149‑1 (MQ=255)
                                 tctTCGGCAATCGCCGGCTGGTTGGCCAGCGGCTTCACCGCCGTGCTCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACg                                                                                                               >  5:334517/1‑149 (MQ=255)
                                   ttCGGCAATCGCCGGCTGGTTGGCCAGCGGCTTCACCGCCGTGCTCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGca                                                                                                             >  5:63064/1‑149 (MQ=255)
                                      ggCAATCGCCGGCTGGTTGGCCAGCGGCTTCACCGCCGTGCTCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTc                                                                                                                  <  7:214656/141‑1 (MQ=255)
                                      ggCAATCGCCGGCTGGTTGGCCAGCGGCTTCACCGCCGTGCTCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTc                                                                                                                  >  8:214656/1‑141 (MQ=255)
                                       gCAATCGCCGGCTGGTTGGCCAGCGGCTTCACCGCCGTGCTCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATg                                                                                                         >  8:193046/1‑149 (MQ=255)
                                         aaTCGCCGGCTGGTTGGCCAGCGGCTTCACCGCCGTGCTCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAgg                                                                                                                                   <  3:36593/121‑1 (MQ=255)
                                         aaTCGCCGGCTGGTTGGCCAGCGGCTTCACCGCCGTGCTCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAgg                                                                                                                                   >  4:36593/1‑121 (MQ=255)
                                         aaTCGCCGGCTGGTTGGCCAGCGGCTTCACCGCCGTGCTCAGCTGTTCGGCCAGCTTATGCAACAGTTGGCCCTGAGACGGCACCGGAGACGTCTCACTGCCCTCGTGCGGCGTCTCGAGGTGGATGCTACGCTTGTAACGcccaggca                                                                                                       >  4:310322/1‑145 (MQ=255)
                                              ccGGCTGGTTGGCCAGCGGCTTCACCGCCGTGCTCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATa                                                                                                                          >  4:225264/1‑125 (MQ=255)
                                              ccGGCTGGTTGGCCAGCGGCTTCACCGCCGTGCTCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATa                                                                                                                          <  3:225264/125‑1 (MQ=255)
                                                ggCTGGTTGGCCAGCGGCTTCACCGCCGTGCTCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCAcggcg                                                                                                <  2:83651/149‑1 (MQ=255)
                                                 gCTGGTTGGCCAGCGGCTTCACCGCCGTGCTCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCAcggcgg                                                                                               <  5:71778/149‑1 (MQ=255)
                                                     gTTGGCCAGCGGCTTCACCGCCGTGCTCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTgg                                                                                                                                >  8:151449/1‑112 (MQ=255)
                                                     gTTGGCCAGCGGCTTCACCGCCGTGCTCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTgg                                                                                                                                <  7:151449/112‑1 (MQ=255)
                                                         gCCAGCGGCTTCACCGCCGTGCTCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCAcggcg                                                                                                >  4:65286/1‑140 (MQ=255)
                                                         gCCAGCGGCTTCACCGCCGTGCTCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCAcggcg                                                                                                <  3:65286/140‑1 (MQ=255)
                                                          ccAGCGGCTTCACCGCCGTGCTCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGa                                                                                      >  1:299146/1‑149 (MQ=255)
                                                          ccAGCGGCTTCACCGCCGTGCTCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGa                                                                                      >  1:206353/1‑149 (MQ=255)
                                                                gCTTCACCGCCGTGCTCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCAc                                                                                >  8:117713/1‑149 (MQ=255)
                                                                      ccgccgTGCTCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCa                                                                          >  1:114502/1‑149 (MQ=255)
                                                                       cgccgTGCTCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCAcg                                                                                                   >  8:84056/1‑123 (MQ=255)
                                                                       cgccgTGCTCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCAcg                                                                                                   <  7:84056/123‑1 (MQ=255)
                                                                            tGCTCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCAc                                                                                                    <  3:324568/117‑1 (MQ=255)
                                                                            tGCTCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCAc                                                                                                    >  4:324568/1‑117 (MQ=255)
                                                                               tCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCATGAGCCTGCACATGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCGCGGCGGTCAAGCAGACCCCACTGGCCATCCAGGATg                                                                 >  6:47365/1‑149 (MQ=255)
                                                                                  gCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGcc                                                              <  5:416682/149‑1 (MQ=255)
                                                                                     tcagggCCAACTGTTGCAGAAGCTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGc                                                           >  5:276174/5‑149 (MQ=255)
                                                                                     gTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGc                                                           >  3:201915/1‑149 (MQ=255)
                                                                                        cGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGc                                                           >  3:71309/1‑146 (MQ=255)
                                                                                        cGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGc                                                           <  4:71309/146‑1 (MQ=255)
                                                                                        cGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGCTgg                                                        <  4:214417/149‑1 (MQ=255)
                                                                                         ggCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGCTGGt                                                       <  8:292641/149‑1 (MQ=255)
                                                                                          gCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGCTGGtt                                                      >  1:220548/1‑149 (MQ=255)
                                                                                          gCCAGCTTCTGCAACAGTTGGCCATGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGCTGGtt                                                      <  8:224204/149‑1 (MQ=255)
                                                                                              gCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGCTGGTTCTTg                                                  <  4:201915/149‑1 (MQ=255)
                                                                                                  cTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGCTGGTTCTTGCCa                                               <  2:299146/148‑1 (MQ=255)
                                                                                                    gCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATgg                                                                >  5:118870/1‑129 (MQ=255)
                                                                                                    gCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATgg                                                                <  6:118870/129‑1 (MQ=255)
                                                                                                    gCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGCTGGTTCTTGCCAGaa                                            >  3:232476/1‑149 (MQ=255)
                                                                                                     cAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGCTGGTTCTTGCCAGAAt                                           >  8:231438/1‑149 (MQ=255)
                                                                                                           ctGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCGCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGCTGGTTCTTGCCAGAATCCAGGc                                     <  6:276174/148‑1 (MQ=255)
                                                                                                              gCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGCTGGTTCTTGCCAGAATCCAGGCgt                                   <  3:56433/148‑1 (MQ=255)
                                                                                                               cccTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGCTGGTTCTTGCCAGAATCCAGGCgtgt                                 <  6:148523/149‑1 (MQ=255)
                                                                                                                ccTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGCTGGTTCTTGCCAGAATCCAGGCgtgt                                 >  6:352273/1‑148 (MQ=255)
                                                                                                                 cTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGCTGGTTCTTGCCAGAATCCAGGCGTGTGa                               >  7:40845/1‑149 (MQ=255)
                                                                                                                      ccTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGCTGGTTCTTGCCAGAATCAAGGCGTGTGATCGa                           >  5:133873/1‑148 (MQ=255)
                                                                                                                          cACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGCTGGTTCTTGCCAGAATCCAGGCGTGTGATCGacaaca                      <  7:117713/149‑1 (MQ=255)
                                                                                                                             cTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGCTGGTTCTTGCCAGAATCCAGGCGTGTGATCGACAACAGCa                   >  6:232072/1‑149 (MQ=255)
                                                                                                                                cgacgaCTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGCTGGTTCTTGCCAGAATCCAGGCGTGTGATCGACAACAGCACt                 <  8:40845/148‑1 (MQ=255)
                                                                                                                                  acgacTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGCTGGTTCTTGCCAGAATCCAGGCGTGTGATCGACAACAGCACTTGc              <  6:63064/149‑1 (MQ=255)
                                                                                                                                      cTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGCTGGTTCTTGCCAGAATCCAGGCGTGTGATCGACAACAGCACTTGCACGt          >  5:157403/1‑149 (MQ=255)
                                                                                                                                       tCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGCCGCCACTGGGCATCCAGGATGGCCGGCTCGTTCTTGCCAGAATCCAGGCGTGTGATCGACAACAGCACTTGCACGTc         >  3:80493/1‑149 (MQ=255)
                                                                                                                                       tCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGCTGGTTCTTGCCAGAATCCAGGCGTGTGATCGa                           <  5:278233/131‑1 (MQ=255)
                                                                                                                                       tCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGCTGGTTCTTGCCAGAATCCAGGCGTGTGATCGa                           >  6:278233/1‑131 (MQ=255)
                                                                                                                                       tCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGCTGGTTCTTGCCAGAATCCAGGCGTGTGATCGACAACAGCACTTGCACGTc         >  1:383914/1‑149 (MQ=255)
                                                                                                                                         aCTGCCCTCGTGCTGCTGCTCGAGGTGTATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGCTGGTTCTTGCCAGAATCCAGGCGTGTGATCGACAACAGCACtt                <  6:267254/140‑1 (MQ=255)
                                                                                                                                         aCTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGc                                                           <  6:133838/97‑1 (MQ=255)
                                                                                                                                         aCTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGc                                                           >  5:133838/1‑97 (MQ=255)
                                                                                                                                         aCTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGCTGGTTCTTGCCAGAATCCAGGCGTGTGATCGACAACAGCa                   <  6:37084/137‑1 (MQ=255)
                                                                                                                                         aCTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGCTGGTTCTTGCCAGAATCCAGGCGTGTGATCGACAACAGCa                   >  5:37084/1‑137 (MQ=255)
                                                                                                                                         aCTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGCTGGTTCTTGCCAGAATCCAGGCGTGTGATCGACAACAGCACtt                >  5:267254/1‑140 (MQ=255)
                                                                                                                                          cTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGCTGGTTCTTGCCAGAATCCAGGCGTGTGATCGACAACAGCACTTGCACGTCCgg      >  4:557/1‑149 (MQ=255)
                                                                                                                                           tGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGCTGGTTCTTGCCAGAATCCAGGCGTGTGATCGACAACAGCACTTGCACGTCCggg     >  6:406249/1‑149 (MQ=255)
                                                                                                                                           ccaccTCGAGCAGCAGCACGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGCTGGTTCTTGCCAGAATCCAGGCGTGTGATCGACAACAGCACTTGc              <  6:92744/137‑1 (MQ=255)
                                                                                                                                           ccaccTCGAGCAGCAGCACGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGCTGGTTCTTGCCAGAATCCAGGCGTGTGATCGACAACAGCACTTGc              >  5:92744/4‑140 (MQ=255)
                                                                                                                                            caccTCGAGCAGCAGCACGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGCTGGTTCTTGCCAGAATCCAGGCGTGAGATCGACAACAGCACTTGCACGTCCGGGc    >  3:125849/3‑149 (MQ=255)
                                                                                                                                             cccTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACAT‑GCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGCTGGTTCTTGCCAGAATCCAGGCGTGTGATCGACAACAGCACTTGCACGTCCGGGCt   >  8:35843/1‑148 (MQ=255)
                                                                                                                                              ccTCGAGCAGCAGCACGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGCTGGTTCTTGCCAGAATCCAGGCGTGTGATCGACAACAGCACTTGCACGTCCGGGCt   >  6:25046/1‑148 (MQ=255)
                                                                                                                                               cTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGCTGGTTCTTGCCAGAATCCAGGCGTGTGATCGACAACAGCACTTGCACGTCCGGGCTg  >  2:322206/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                               
ACCTGAGTCGGTGCAGCGGGCTTTTTCGGTGCCTCTTCGGCAATCGCCGGCTGGTTGGCCAGCGGCTTCACCGCCGTGCTCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGCTGGTTCTTGCCAGAATCCAGGCGTGTGATCGACAACAGCACTTGCACGTCCGGGCTG  >  NC_002947/5582666‑5582956

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑