Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,582,822 T→A 6.8% E175V (GAG→GTG)  PP_4911 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,582,8220TA 6.8% 245.6 / 7.4 88E175V (GAG→GTG) PP_4911hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (47/34);  new base A (4/2);  total (51/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.22e-01

GTGCAGCGGGCTTTTTCGGTGCCTCTTCGGCAATCGCCGGCTGGTTGGCCAGCGGCTTCACCGCCGTGCTCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGCTGGTTCTTGCCAGAATCCAGGCGTGTGATCGACAACAGCACTTGCACGTCCGGGCTGAAGCCTGCGGTC  >  NC_002947/5582676‑5582968
                                                                                                                                                  |                                                                                                                                                  
gTGCAGCGGGCTTTTTCGGTGCCTCTTCGGCAATCGCCGGCTGGTTGGCCAGCGGCTTCACCGCCGTGCTCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTc                                                                                                                                                   >  1:83651/1‑148 (MQ=255)
   cAGCGGGCTTTTTCGGTGCCTCTTCGGCAATCGCCGGCTGGTTGGCCAGCGGCTTCACCGCCGTGCTCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAgg                                                                                                                                               <  5:391813/149‑1 (MQ=255)
   cAGCGGGCTTTTTCGGTGCCTCTTCGGCAATCGCCGGCTGGTTGGCCAGCGGCTTCACCGCCGTGCTCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAg                                                                                                                                                <  6:141136/148‑1 (MQ=255)
      cGGGCTTTTTCGGTGCCTCTTCGGCAATCGCCGGCTGGTTGGCCAGCGGCTTCACCGCCGTGCTCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTgg                                                                                                                                            <  5:399522/149‑1 (MQ=255)
      cGGGCTTTTTCGGTGCCTCTTCGGCAATCGCCGGCTGGTTGGCCAGCGGCTTCACCGCCGTGCTCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTg                                                                                                                                             >  7:333337/1‑148 (MQ=255)
         gCTTTTTCGGTGCCTCTTCGGCAATCGCCGGCTGGTTGGCCAGCGGCTTCACCGCCGTGCTCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGgat                                                                                                                                          >  6:71778/1‑148 (MQ=255)
          cTTTTTCGGTGCCTCTTCGGCATTGGCCGGGTGGTTGGCCAGCGGCTTCACCGCCGTGCTCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGgatga                                                                                                                                        <  8:333337/149‑1 (MQ=255)
           tttttCGGTGCCTCTTCGGCAATCGCCGGCTGGTTGGCCAGCGGCTTCACCGCCGTGCTCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGCCTCACTGCCCTCGTGCTGCTGCTCGAGGTgactac                                                                                                                                        >  5:234203/1‑143 (MQ=255)
           tttttCGGTGCCTCTTCGGCAATCGCCGGCTGGTTGGCCAGCGGCTTCACCGCCGTGCTCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGgatga                                                                                                                                        >  6:416682/1‑148 (MQ=255)
           tttttCGGTGCCTCTTCGGCAATCGCCGGCTGGTTGGCCAGCGGCTTCACCGCCGTGCTCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGgatga                                                                                                                                        >  3:214417/1‑148 (MQ=255)
             tttCGGTGCCTCTTCGGCAATCGCCGGCTGGTTGGCCAGCGGCTTCACCGCCGTGCTCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATa                                                                                                                                      <  1:409091/148‑1 (MQ=255)
                cGGTGCCTCTTCGGCAATCGCCGGCTGATTGGCCAGCGGCTTCACCGCCGTGCTCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGt                                                                                                                                  >  1:133284/1‑149 (MQ=255)
                 ggTGCCTCTTCGGCAATCGCCGGCTGGTTGGCCAGCGGCTTCACCGCCGTGCTCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGtt                                                                                                                                 <  2:40281/149‑1 (MQ=255)
                 ggTGCCTCTTCGGCAATCGCCGGCTGGTTGGCCAGCGGCTTCACCGCCGTGCTCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGtt                                                                                                                                 <  1:273620/149‑1 (MQ=255)
                  gTGCCTCTTCGGCAATCGCCGGCTGGTTGGCCAGCGGCTTCACCGCCGTGCTCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATCCGGTTg                                                                                                                                >  2:141625/1‑149 (MQ=255)
                  gTGCCTCTTCGGCAATCGCCGGCTGGTTGGCCAGCGGCTTCACCGCCGTGCTCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTg                                                                                                                                >  7:292641/1‑149 (MQ=255)
                  gTGCCTCTTCGGCAATCGCCGGCTGGTTGGCCAGCGGCTTCACCGCCGTGCTCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGAGCAGCAGCACGAgg                                                                                                                                               >  6:137707/1‑134 (MQ=255)
                  gTGCCTCTTCGGCAATCGCCGGCTGGTTGGCCAGCGGCTTCACCGCCGTGCTCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGAGCAGCAGCACGAgg                                                                                                                                               <  5:137707/134‑1 (MQ=255)
                      ctctTCGGCAATCGCCGGCTGGTTGGCCAGCGGCTTCACCGCCGTGCTCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCAc                                                                                                                            <  1:244189/149‑1 (MQ=255)
                       tctTCGGCAATCGCCGGCTGGTTGGCCAGCGGCTTCACCGCCGTGCTCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACg                                                                                                                           >  5:334517/1‑149 (MQ=255)
                         ttCGGCAATCGCCGGCTGGTTGGCCAGCGGCTTCACCGCCGTGCTCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGca                                                                                                                         >  5:63064/1‑149 (MQ=255)
                            ggCAATCGCCGGCTGGTTGGCCAGCGGCTTCACCGCCGTGCTCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTc                                                                                                                              >  8:214656/1‑141 (MQ=255)
                            ggCAATCGCCGGCTGGTTGGCCAGCGGCTTCACCGCCGTGCTCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTc                                                                                                                              <  7:214656/141‑1 (MQ=255)
                             gCAATCGCCGGCTGGTTGGCCAGCGGCTTCACCGCCGTGCTCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATg                                                                                                                     >  8:193046/1‑149 (MQ=255)
                               aaTCGCCGGCTGGTTGGCCAGCGGCTTCACCGCCGTGCTCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAgg                                                                                                                                               <  3:36593/121‑1 (MQ=255)
                               aaTCGCCGGCTGGTTGGCCAGCGGCTTCACCGCCGTGCTCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAgg                                                                                                                                               >  4:36593/1‑121 (MQ=255)
                               aaTCGCCGGCTGGTTGGCCAGCGGCTTCACCGCCGTGCTCAGCTGTTCGGCCAGCTTATGCAACAGTTGGCCCTGAGACGGCACCGGAGACGTCTCACTGCCCTCGTGCGGCGTCTCGAGGTGGATGCTACGCTTGTAACGcccaggca                                                                                                                   >  4:310322/1‑145 (MQ=255)
                                    ccGGCTGGTTGGCCAGCGGCTTCACCGCCGTGCTCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATa                                                                                                                                      <  3:225264/125‑1 (MQ=255)
                                    ccGGCTGGTTGGCCAGCGGCTTCACCGCCGTGCTCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATa                                                                                                                                      >  4:225264/1‑125 (MQ=255)
                                      ggCTGGTTGGCCAGCGGCTTCACCGCCGTGCTCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCAcggcg                                                                                                            <  2:83651/149‑1 (MQ=255)
                                       gCTGGTTGGCCAGCGGCTTCACCGCCGTGCTCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCAcggcgg                                                                                                           <  5:71778/149‑1 (MQ=255)
                                           gTTGGCCAGCGGCTTCACCGCCGTGCTCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTgg                                                                                                                                            >  8:151449/1‑112 (MQ=255)
                                           gTTGGCCAGCGGCTTCACCGCCGTGCTCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTgg                                                                                                                                            <  7:151449/112‑1 (MQ=255)
                                               gCCAGCGGCTTCACCGCCGTGCTCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCAcggcg                                                                                                            <  3:65286/140‑1 (MQ=255)
                                               gCCAGCGGCTTCACCGCCGTGCTCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCAcggcg                                                                                                            >  4:65286/1‑140 (MQ=255)
                                                ccAGCGGCTTCACCGCCGTGCTCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGa                                                                                                  >  1:299146/1‑149 (MQ=255)
                                                ccAGCGGCTTCACCGCCGTGCTCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGa                                                                                                  >  1:206353/1‑149 (MQ=255)
                                                      gCTTCACCGCCGTGCTCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCAc                                                                                            >  8:117713/1‑149 (MQ=255)
                                                            ccgccgTGCTCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCa                                                                                      >  1:114502/1‑149 (MQ=255)
                                                             cgccgTGCTCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCAcg                                                                                                               <  7:84056/123‑1 (MQ=255)
                                                             cgccgTGCTCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCAcg                                                                                                               >  8:84056/1‑123 (MQ=255)
                                                                  tGCTCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCAc                                                                                                                <  3:324568/117‑1 (MQ=255)
                                                                  tGCTCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCAc                                                                                                                >  4:324568/1‑117 (MQ=255)
                                                                     tCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCATGAGCCTGCACATGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCGCGGCGGTCAAGCAGACCCCACTGGCCATCCAGGATg                                                                             >  6:47365/1‑149 (MQ=255)
                                                                        gCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGcc                                                                          <  5:416682/149‑1 (MQ=255)
                                                                           tcagggCCAACTGTTGCAGAAGCTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGc                                                                       >  5:276174/5‑149 (MQ=255)
                                                                           gTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGc                                                                       >  3:201915/1‑149 (MQ=255)
                                                                              cGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGc                                                                       >  3:71309/1‑146 (MQ=255)
                                                                              cGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGc                                                                       <  4:71309/146‑1 (MQ=255)
                                                                              cGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGCTgg                                                                    <  4:214417/149‑1 (MQ=255)
                                                                               ggCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGCTGGt                                                                   <  8:292641/149‑1 (MQ=255)
                                                                                gCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGCTGGtt                                                                  >  1:220548/1‑149 (MQ=255)
                                                                                gCCAGCTTCTGCAACAGTTGGCCATGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGCTGGtt                                                                  <  8:224204/149‑1 (MQ=255)
                                                                                    gCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGCTGGTTCTTg                                                              <  4:201915/149‑1 (MQ=255)
                                                                                        cTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGCTGGTTCTTGCCa                                                           <  2:299146/148‑1 (MQ=255)
                                                                                          gCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATgg                                                                            >  5:118870/1‑129 (MQ=255)
                                                                                          gCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATgg                                                                            <  6:118870/129‑1 (MQ=255)
                                                                                          gCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGCTGGTTCTTGCCAGaa                                                        >  3:232476/1‑149 (MQ=255)
                                                                                           cAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGCTGGTTCTTGCCAGAAt                                                       >  8:231438/1‑149 (MQ=255)
                                                                                                 ctGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCGCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGCTGGTTCTTGCCAGAATCCAGGc                                                 <  6:276174/148‑1 (MQ=255)
                                                                                                    gCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGCTGGTTCTTGCCAGAATCCAGGCgt                                               <  3:56433/148‑1 (MQ=255)
                                                                                                     cccTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGCTGGTTCTTGCCAGAATCCAGGCgtgt                                             <  6:148523/149‑1 (MQ=255)
                                                                                                      ccTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGCTGGTTCTTGCCAGAATCCAGGCgtgt                                             >  6:352273/1‑148 (MQ=255)
                                                                                                       cTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGCTGGTTCTTGCCAGAATCCAGGCGTGTGa                                           >  7:40845/1‑149 (MQ=255)
                                                                                                            ccTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGCTGGTTCTTGCCAGAATCAAGGCGTGTGATCGa                                       >  5:133873/1‑148 (MQ=255)
                                                                                                                cACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGCTGGTTCTTGCCAGAATCCAGGCGTGTGATCGacaaca                                  <  7:117713/149‑1 (MQ=255)
                                                                                                                   cTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGCTGGTTCTTGCCAGAATCCAGGCGTGTGATCGACAACAGCa                               >  6:232072/1‑149 (MQ=255)
                                                                                                                      cgacgaCTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGCTGGTTCTTGCCAGAATCCAGGCGTGTGATCGACAACAGCACt                             <  8:40845/148‑1 (MQ=255)
                                                                                                                        acgacTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGCTGGTTCTTGCCAGAATCCAGGCGTGTGATCGACAACAGCACTTGc                          <  6:63064/149‑1 (MQ=255)
                                                                                                                            cTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGCTGGTTCTTGCCAGAATCCAGGCGTGTGATCGACAACAGCACTTGCACGt                      >  5:157403/1‑149 (MQ=255)
                                                                                                                             tCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGCCGCCACTGGGCATCCAGGATGGCCGGCTCGTTCTTGCCAGAATCCAGGCGTGTGATCGACAACAGCACTTGCACGTc                     >  3:80493/1‑149 (MQ=255)
                                                                                                                             tCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGCTGGTTCTTGCCAGAATCCAGGCGTGTGATCGa                                       >  6:278233/1‑131 (MQ=255)
                                                                                                                             tCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGCTGGTTCTTGCCAGAATCCAGGCGTGTGATCGa                                       <  5:278233/131‑1 (MQ=255)
                                                                                                                             tCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGCTGGTTCTTGCCAGAATCCAGGCGTGTGATCGACAACAGCACTTGCACGTc                     >  1:383914/1‑149 (MQ=255)
                                                                                                                               aCTGCCCTCGTGCTGCTGCTCGAGGTGTATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGCTGGTTCTTGCCAGAATCCAGGCGTGTGATCGACAACAGCACtt                            <  6:267254/140‑1 (MQ=255)
                                                                                                                               aCTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGc                                                                       >  5:133838/1‑97 (MQ=255)
                                                                                                                               aCTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGc                                                                       <  6:133838/97‑1 (MQ=255)
                                                                                                                               aCTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGCTGGTTCTTGCCAGAATCCAGGCGTGTGATCGACAACAGCa                               >  5:37084/1‑137 (MQ=255)
                                                                                                                               aCTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGCTGGTTCTTGCCAGAATCCAGGCGTGTGATCGACAACAGCa                               <  6:37084/137‑1 (MQ=255)
                                                                                                                               aCTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGCTGGTTCTTGCCAGAATCCAGGCGTGTGATCGACAACAGCACtt                            >  5:267254/1‑140 (MQ=255)
                                                                                                                                cTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGCTGGTTCTTGCCAGAATCCAGGCGTGTGATCGACAACAGCACTTGCACGTCCgg                  >  4:557/1‑149 (MQ=255)
                                                                                                                                 tGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGCTGGTTCTTGCCAGAATCCAGGCGTGTGATCGACAACAGCACTTGCACGTCCggg                 >  6:406249/1‑149 (MQ=255)
                                                                                                                                 ccaccTCGAGCAGCAGCACGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGCTGGTTCTTGCCAGAATCCAGGCGTGTGATCGACAACAGCACTTGc                          <  6:92744/137‑1 (MQ=255)
                                                                                                                                 ccaccTCGAGCAGCAGCACGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGCTGGTTCTTGCCAGAATCCAGGCGTGTGATCGACAACAGCACTTGc                          >  5:92744/4‑140 (MQ=255)
                                                                                                                                  caccTCGAGCAGCAGCACGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGCTGGTTCTTGCCAGAATCCAGGCGTGAGATCGACAACAGCACTTGCACGTCCGGGc                >  3:125849/3‑149 (MQ=255)
                                                                                                                                   cccTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACAT‑GCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGCTGGTTCTTGCCAGAATCCAGGCGTGTGATCGACAACAGCACTTGCACGTCCGGGCt               >  8:35843/1‑148 (MQ=255)
                                                                                                                                    ccTCGAGCAGCAGCACGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGCTGGTTCTTGCCAGAATCCAGGCGTGTGATCGACAACAGCACTTGCACGTCCGGGCt               >  6:25046/1‑148 (MQ=255)
                                                                                                                                     cTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGCTGGTTCTTGCCAGAATCCAGGCGTGTGATCGACAACAGCACTTGCACGTCCGGGCTg              >  2:322206/1‑148 (MQ=255)
                                                                                                                                              ctgctCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGCTGGTTCTTGCCAGAATCCAGGCGTGTGATCGACAACAGCACTTGCACGTCCGGGCTGAAGCCTGCgg    >  7:22722/1‑149 (MQ=255)
                                                                                                                                              ctgctCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGCTGGTTCTTGCCAGAATCCAGGCGTGTGATCGACAACAGCACTTGCACGTCCGGGCTGAAGCCTGCg     <  7:231438/148‑1 (MQ=255)
                                                                                                                                              ctgctCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGCTGGTTCTTGCCAGAATCCAGGCGTGTGATCGACAACAGCACTTGCACGTCCGGGCTGAAGCCTGCg     <  1:141625/148‑1 (MQ=255)
                                                                                                                                              ctgctCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGCTGGTTCTCGCCAGAATCCAGGCGTGTGATCGACAACAGCACTTGCACGTCCGGGCTGAAGCCTGCgg    >  3:173311/1‑149 (MQ=255)
                                                                                                                                                 ctCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGCTGGTTCTTGCCAGAATCCAGGCGTGTGATCGACAACAGCACTTGCACGTCCGGGCTGAAGCCTGCGGTc  <  2:220548/148‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
GTGCAGCGGGCTTTTTCGGTGCCTCTTCGGCAATCGCCGGCTGGTTGGCCAGCGGCTTCACCGCCGTGCTCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGCTGGTTCTTGCCAGAATCCAGGCGTGTGATCGACAACAGCACTTGCACGTCCGGGCTGAAGCCTGCGGTC  >  NC_002947/5582676‑5582968

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑