Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,613,535	T→A	7.8%	G162G (GGT→GGA)	PP_4933 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,613,535	0	T	A	7.8%	223.5 / 7.5	77	G162G (GGT→GGA)	PP_4933	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (37/34);  new base A (2/4);  total (39/38)
Fisher's exact test for biased strand distribution p-value = 4.31e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01

TACAGGAAGTGGCCGAGGAGTGCCTGCTGGAAACCCCGGAAGGTTGGCTGGGCGGGCGCTTCAACGACACCTGGCTGCCCATCCCCTACGCCGCCGCCCTGCACTACCGCGAAACACCCCACTTCGTGCTGGCTCCGGAGTCCGGTGCC‑GCACGCCCGGTGCATTGCGGCAAACTGATGCTACTGGAGCGCCCACGGGCCTATGTGCACCTGCCCACCGCTTCGCTACAGCTGATCTATGACATGCGCCTGCAAGTGCCCCGGGACGCCAAGAAACTCAGCTTGTTCCACGT  >  NC_002947/5613390‑5613681                                                                                                                                                  |                                                                                                                                                    tACAGGAAGTGGCCGAGGAGTGCCTGCTGGAAACCCCGGAAGGTTGGCTGGGCGGGCGCTTCAACGACACCTGGCTGCCCATCCCCTACGCCGCCGCCCTGCACTACCGCGAAACACCCCACTTCGTGCTGGCTCCGGAGTCCGGTGcc                                                                                                                                                  <  6:26928/149‑1 (MQ=255)   cAGGAAGTGGCCGAGGAGTGCCTGCTGGAAACCCCGGAAGGTTGGCTGGGCGGGCGCTTCAACGACACCTGGCTGCCCATCCCCTACGCCGCCGCCCTGCACTACCGCGAAACACCCCACTTCGTGCTGGCACCGGACTCCGGAGccag                                                                                                                                                <  5:255938/149‑3 (MQ=255)   cAGGAAGTGGCCGAGGAGTGCCTGCTGGAAACCCCGGAAGGTTGGCTGGGCGGGCGCTTCAACGACACCTGGCTGCCCATCCCCTACGCCGCCGCCCTGCACTACCGCGAAACACCCCACTTCGTGCTGGCACCGGACTCCGGAGcca                                                                                                                                                 <  4:282630/148‑2 (MQ=255)    aGGAAGTGGCCGAGGAGTGCCTGCTGGAAACCCCGGAAGGTTGGCTGGGCGGGCGCTTCAACGACACCTGGCTGCCCATCCCCTACGCCGCCGCCCTGCACTACCGCGAAACACCCCACTTCGTGCTGGCTCCGGAGTCCGGTGCC‑GCa                                                                                                                                              >  1:94057/1‑149 (MQ=255)      gAAGTGGCCGAGGAGTGCCTGCTGGAAACCCCGGAAGGTTGGCTGGGCGGGCGCTTCAACGACACCTGGCTGCCCATCCCCTACGCCGCCGCCCTGCACTACCGCGAAACACCCCACTTCGTGCTGGCACCGGACTCCGGAGccag                                                                                                                                                <  4:123768/146‑3 (MQ=255)      gAAGTGGCCGAGGAGTGCCTGCTGGAAACCCCGGAAGGTTGGCTGGGCGGGCGCTTCAACGACACCTGGCTGCCCATCCCCTACGCCGCCGCCCTGCACTACCGCGAAACACCCCACTTCGTGCTGGCACCGGACTCCGGAGccag                                                                                                                                                >  3:123768/1‑144 (MQ=255)        aGTGGCCGAGGAGTGCCTGCTGGAAACCCCGGAAGGTTGGCTGGGCGGGCGCTTCAACGACACCTGGCTGCCCATCCCCTACGCCGCCGCCCTGCATTACCGCGAAACACCCCACTTCGTGCTGGCTCCGGAGTCCGGTGCC‑GCACGcc                                                                                                                                          <  6:419561/149‑1 (MQ=255)        aGTGGCCGAGGAGTGCCTGCTGGAAACCCCGGAAGGTTGGCTGGGCGGGCGCTTCAACGACACCTGGCTGCCCATCCCCTACGCCGCCGCCCTGCACTACCGCGAAACACCCCACTTCGTGC‑GGCACCGGACTCCGGAGCCAGCACgaa                                                                                                                                          <  2:294184/149‑3 (MQ=255)          tGGCCGAGGAGTGCCTGCTGGAAACCCCGGAAGGTTGGCTGGGCGGGCGCTTCAACGACACCTGGCTGCCCATCCCCTACGCCGCCGCCCTGCACTACCGCGAAACACCCCACTTCGTGCTGGCTCCGGAGTCCGGTGCC‑GCACGCCCg                                                                                                                                        >  6:6204/1‑149 (MQ=255)          tGGCCGAGGAGTGCCTGCTGGAAACCCCGGAAGGTTGGCTGGGCGGGCGCTTCAACGACACCTGGCTGCCCATCCCCTACGCCGCCGCCCTGCACTACCGCGAAACACCCCACTTCGTGCTGGCTCCGGAGTCCGGTGCC‑GCACGCCCg                                                                                                                                        <  1:143312/149‑1 (MQ=255)            gCCGAGGAGTGCCTGCTGGAAACCCCGGAAGGTTGGCTGGGCGGGCGCTTCAACGACACCTGGCTGCCCATCCCCTACGCCGCCGCCCTGCACTACCGCGAAACACCCCACTTCGTGCTGGCTCCGGAGTCCGGTGCC‑GCACGCCCGGt                                                                                                                                      >  5:365106/1‑149 (MQ=255)                  gagTGCCTGCTGGAAACCCCGGAAGGTTGGCTGGGCGGGCGCTTCAACGACACCTGGCTGCCCATCCCCTACGCCGCCGCCCTGCACTACCGCGAAACACCCCACTTCGTGCTGGCTCCGGAGTCCGGTGCC‑GCACGCCCGGTGCATTg                                                                                                                                <  4:61056/149‑1 (MQ=255)                     tGCCTGCTGGAAACCCCGGAAGGTTGGCTGGGCGGGCGCTTCAACGACACCTGGCTGCCCATCCCCTACGCCGCCGCCCTGCACTACCGCGAAACACCCCACTTCGTGCTGGCTCCGGAGTCCGGTGCC‑GCACGCCCGGTGCATTGCgg                                                                                                                             >  2:360904/1‑149 (MQ=255)                           ctgGAAACCCCGGAAGGTTGGCTGGGCGGGCGCTTCAACGACACCTGGCTGCCCATCCCCTACGCCGCCGCCCTGCACTACCGCGAAACACCCCACTTCGTGCTGGCTCCGGAGTCCGGTGCC‑GCACGCCCGGTGCATTGCGGCAAACt                                                                                                                       <  6:140114/149‑1 (MQ=255)                           ctcgcacccccGCAAGGTTGGCTGGTCGCTCCCCTCCACGACACCTCTCTGCCCATCCCCTCCCCCGCCGCCCTGCACTACCGCGACACACCCCAGATCGTGCTGGCTCCGGAGTCCGGTGCC‑GCACGCCCGGTGCATTGCGGCaaa                                                                                                                         <  4:304947/140‑1 (MQ=255)                            tgGAAACCCCGGAAGGTTGGCTGGGCGGGCGCTTCAACGACACCTGGCTGCCCATCCCCTACGCCGCCGCCCTGCACTACCGCGAAACACCCCACTTCGTGCTGGCTCCGGAGTCCGGTGCC‑GCACGCCCGGTGCATTGCGGTAAACt                                                                                                                       >  3:297247/1‑148 (MQ=255)                              gAAACCCCGGAAGGTTGGCTGGGCGGGCGCTTCAACGACACCTGGCTGCCCATCCCCTACGCCGCCGCCCTGCACTACCGCGAAACACCCCACTTCGTGCTGGCTCCGGAGTCCGGTGCC‑GCACGCCCGGTGCATTGCGGCAAACTGAt                                                                                                                    >  5:238423/1‑149 (MQ=255)                              gAAACCCCGGAAGGTTGGCTGGGCGGGCGCTTCAACGACACCTGGCTGCCCATCCCCTACGCCGCCGCCCTGCACTACCGCGAAACACCCCACTTCGTGCTGGCTCCGGAGTCCGGTGCC‑GCACGCCCGGTGCATTGCGGCAAACTGAt                                                                                                                    <  1:360904/149‑1 (MQ=255)                               aaaCCCCGGAAGGTTGGCTGGGCGGGCGCTTCAACGACACCTGGCTGCCCATCCCCTACGCCGCCGCCCTGCACTACCGCGAAACACCCCACTTCGTGCTGGCTCCGGAGTCCGGTGCC‑GCACGCCCGGTGCATTGCGGCAAACTGATg                                                                                                                   <  7:239573/149‑1 (MQ=255)                                  ccccGGAAGGTTGGCTGGGCGGGCGCTTCAACGACACCTGGCTGCCCATCCCCTACGCCGCCGCCCTGCACTACCGCGAAACACCCCACTTCGTGCTGGCTCCGGAGTCCGGTGCC‑GCACGCCCGGTGCATTGCGGCAAACTGATGCTa                                                                                                                >  6:135457/1‑149 (MQ=255)                                   cccGGAAGGTTGGCTGGGCGGGCGCTTCAACGACACCTGGCTGCCCATCCCCTACGCCGCCGCCCTGCACTACCGCGAAACACCCCACTTCGTGCTGGCTCCGGAGTCCGGTGCC‑GCACGCCCGGTGCATTGCGGCAAACTGATGCTAc                                                                                                               <  6:365106/149‑1 (MQ=255)                                     cGGAAGGTTGGCTGGGCGGGCGCTTCAACGACACCTGGCTGCCCATCCCCTACGCCGCCGCCCTGCACTACCGCGAAACACCCCACTTCGTGCTGGCTCCGGAGTCCGGTGCC‑GCACGCCCGGTGCATTGCGGCAAACTGATGCTACTg                                                                                                             <  5:6204/149‑1 (MQ=255)                                                  gggcgggcGCTTCAACGACACCTGGCTGCCCATCCCCTACGCCGCCGCCCTGCACTACCGCGAAACACCCCACTTCGTGCTGGCTCCGGAGTCCGGTGCC‑GCACGCCCGGTGCATTGCGGCAAACTGATGCTACTGGAGCGCCCACggg                                                                                                >  6:264068/1‑149 (MQ=255)                                                    gcgggcgCTTCAACGACACCTGGCTGCCCATCCCCTACGCCGCCGCCCTGCACTACCGCGAAACACCCCACTTCGTGCTGGCTCCGGAGTCCGGTGCC‑GCACGCCCGGTGCATTGCGGCAAACTGATGCTACTGGAGCGCCCACGGGcc                                                                                              <  6:238423/149‑1 (MQ=255)                                                     cgggcgCTTCAACGACACCTGGCTGCCCATCCCCTACGCCGCCGCCCTGCACTACCGCGAAACACCCCACTTCGTGCTGGCTCCGGAGTCCGGTGCC‑GCACGCCCGGTGCATTGCGGCAAACTGATGCTACTGGAGCGCCCACGGGCCt                                                                                             <  1:43133/149‑1 (MQ=255)                                                          gcTTCAACGACACCTGGCTGCCCATCCCCTACGCCGCCGCCCTGCACTACCGCGAAACACCCCACTTCGTGCTGGCTCCGGAGTCCGGTGCC‑GCACGCCCGGTGCATTGCGGCAAACTGATGCTACTGGAGCGCCCACGGGCCTAtgtg                                                                                        <  5:135457/149‑1 (MQ=255)                                                            ttCAACGACACCTGGCTGCCCATCCCCTACGCCGCCGCCCTGAACTACCGCGAAACACCCCACTTCGTGCTGGCTCCGGAGTCCGGTGCC‑GCACGCCCGGTGCATTGCGGCAAACTGATGCTACTGGAGCGCCCACGGGCCTATGTGCa                                                                                      >  1:281790/1‑149 (MQ=255)                                                               aaCGACACCTGGCTGCCCATCCCCTACGCCGCCGCCCTGCACTACCGCGAAACACCCCCCTTCGTGCTGGCTCCGGAGTCCGGTGCC‑GCACGCCCGGTGCATTGCGGCAAACTGATGCTACTGGAGCGCCCACGGGCCTATGTGCACCt                                                                                   >  6:21607/1‑149 (MQ=255)                                                                aCGACACCTGGCTGCCCATCCCCTACGCCGCCGCCCTGCACTACCGCGAAACACCCCACTTCGTGCTGGCTCCGGAGTCCGGTGCC‑GCACGCCCGGTGCATTGCGGCAAACTGATGCTACTGGAGCGCCCACGGGCCTATGTGCACCTg                                                                                  >  2:37170/1‑149 (MQ=255)                                                                aCGACACCTGGCTGCCCATCCCCTACGCCGCCGCCCTGCACTACCGCGAAACACCCCACTTCGTGCTGGCTCCGGAGTCCGGTGCC‑GCACGCCCGGTGCATTGCGGCAAACTGATGCTACTGGAGCGCCCACGGCCCTATGTGCACCTg                                                                                  >  8:115106/1‑149 (MQ=255)                                                                 cGACACCTGGCTGCCCATCCCCTACGCCGCCGCCCTGCACTACCGCGAAACACCCCACTTCGTGCTGGCTCCGGAGTCCGGTGCC‑GCACGCCCGGTGCATTGCGGCAAACTGATGCTACTGGAGCGCCCACGGGCCTAt                                                                                           <  1:271494/139‑1 (MQ=255)                                                                 cGACACCTGGCTGCCCATCCCCTACGCCGCCGCCCTGCACTACCGCGAAACACCCCACTTCGTGCTGGCTCCGGAGTCCGGTGCC‑GCACGCCCGGTGCATTGCGGCAAACTGATGCTACTGGAGCGCCCACGGGCCTAt                                                                                           >  2:271494/1‑139 (MQ=255)                                                                       cTGGCTGCCCATCCCCTACGCCGCCGCCCTGCACTACCGCGAAACACCCCACTTCGTGCTGGCTCCGGAGTCCGGTGCC‑GCACGCCCGGTGCATTGCg                                                                                                                              <  8:151394/98‑1 (MQ=255)                                                                       cTGGCTGCCCATCCCCTACGCCGCCGCCCTGCACTACCGCGAAACACCCCACTTCGTGCTGGCTCCGGAGTCCGGTGCC‑GCACGCCCGGTGCATTGCg                                                                                                                              >  7:151394/1‑98 (MQ=255)                                                                          tcTGCGCATCCCCTACGCCGACGCCCTGCACTACCGAGAAAGACCCCACTTCGTGATTGCTCAGGAGTCCGGTGCC‑GCACTCCCGGTGCATGGCGGCAACCTGATGCTACTGGCGCTCCCACGGGCCTATGTGCACCTGCCCACCGCtt                                                                        <  1:151154/148‑1 (MQ=255)                                                                          gCTGCCCATCCCCTACGCCGCCGCCCTGCACTACCGCGAAACACCCCACTTCGTGCTGGCTCCGGAGTCCGGTGCC‑GCACGCCCGGTGCATTGCGGCAAACTGATGCTACTGGAGCGCGCACGGGCCTATGTGCACCTGCCCACCGCtt                                                                        <  1:37170/149‑1 (MQ=255)                                                                               ccATCCCCTACGCCGCCGCCCTGCACTACCGCGAAACACCCCACTTCGTGCTGGCTCCGGAGTCCGGTGCC‑GCACGCCCGGTGCATTGCGGCAAACTGATGCTACTGGAGCGCCCACGGGCCTATGTGCACCTGCCCACCGCTTCg                                                                      >  2:20278/1‑146 (MQ=255)                                                                               ccATCCCCTACGCCGCCGCCCTGCACTACCGCGAAACACCCCACTTCGTGCTGGCTCCGGAGTCCGGTGCC‑GCACGCCCGGTGCATTGCGGCAAACTGATGCTACTGGAGCGCCCACGGGCCTATGTGCACCTGCCCACCGCTTCg                                                                      <  1:20278/146‑1 (MQ=255)                                                                               ccATCCCCTACGCCGCCGCCCTGCACTACCGCGAAACACCCCACTTCGTGCTGGCTCCGGAGTCCGGTGCC‑GCACGCCCGGTGCATTGCGGCAAACTGATGCTACTGGAGCGCCCACGGGCCTATGTGCACCTGCCCACCGCTTCGCTa                                                                   <  7:318909/149‑1 (MQ=255)                                                                                 aTCCCCTACGCCGCCGCCCTGCACTACCGCGAAACACCCCACTTCGTGCTGGCTCCGGAGTCCGGTGCC‑GCACGCCCGGTGCATTGCGGCAAACTGATGCTACTGGAGCGCCCACGGGCCTAtgt                                                                                         <  6:296052/125‑1 (MQ=255)                                                                                 aTCCCCTACGCCGCCGCCCTGCACTACCGCGAAACACCCCACTTCGTGCTGGCTCCGGAGTCCGGTGCC‑GCACGCCCGGTGCATTGCGGCAAACTGATGCTACTGGAGCGCCCACGGGCCTAtgt                                                                                         >  5:296052/1‑125 (MQ=255)                                                                                    cccTACGCCGCCGCCCTGCACTACCGCGAAACACCCCACTTCGTGCTGGCTCCGGAGTCCGGTGCC‑GCACGCCCGGTGCATTGCGGCAAAATGATCATACTGAAGCGCCCACGGGCAAATGTGCACCTGCCCACCCCTTCGCAACAgat                                                              >  8:176918/1‑147 (MQ=255)                                                                                     ccTACGCCGCCGCCCTGCACTACCGCGAAACACCCCACTTCGTGCTGGCTCCGGAGTCCGGTGCC‑GCACGCCCGGTGCATTGCGGCAAACTGATGCTACTGGAGCGCCCACGGGCCTATGTGCACCTGCCCACCGCTTCGCTACAGCt                                                              >  7:169688/1‑148 (MQ=255)                                                                                        aCGCCGCCGCCCTGCACTACCGCGAAACACCCCACTTCGTGCTGGCTCCGGAGTCCGGTGCC‑GCACGCCCGGTGCATTGCGGCAAACTGATGCTACTGGAGCGCCCa                                                                                                    >  4:359489/1‑107 (MQ=255)                                                                                        aCGCCGCCGCCCTGCACTACCGCGAAACACCCCACTTCGTGCTGGCTCCGGAGTCCGGTGCC‑GCACGCCCGGTGCATTGCGGCAAACTGATGCTACTGGAGCGCCCa                                                                                                    <  3:359489/107‑1 (MQ=255)                                                                                        aCGCCGCCGCCCTGCACTACCGCGAAACACCCCACTTCGTGCTGGCTCCGGAGTCCGGTGCC‑GCACGCCCGGTGCATTGCGGCAAACTGATGCTACTGGAGCGCCCACGGGCCTATGTGCACCTGCCCACCGCTTCGCTACAGCTGATc                                                          >  2:212947/1‑149 (MQ=255)                                                                                          gccgccgccCTGCACTACCGCGAAACACCCCACTTCGTGCTGGCTCCGGAGTCCGGTGCC‑GCACGCCCGGTGCATTGCGACAAACTGATGCTACTGGAGCGCCCACGGGCCTATGTGCACCTGCCCACCGCTTCGCTACAGCTGATCTa                                                        >  5:372898/1‑149 (MQ=255)                                                                                            cgccgccCTGCACTACCGCGAAACACCCCACTTCGTGCTGGCTCCGGAGTCCGGTGCC‑GCACGCCCGGTGCATTGCGGCAAACTGATGCTACTGGAGCGCCCACGGGCCTATGTGCACCTGCCCACCGCTTCGCTACAGCTGATCTATg                                                      <  2:281790/149‑1 (MQ=255)                                                                                                  ccTGCACTACCGCGAAACACCCCACTTCGTGCTGGCTCCGGAGTCCGGTGCC‑GCACGCCCGGTGCATTGCGGc                                                                                                                            >  3:276899/1‑73 (MQ=255)                                                                                                  ccTGCACTACCGCGAAACACCCCACTTCGTGCTGGCTCCGGAGTCCGGTGCC‑GCACGCCCGGTGCATTGCGGc                                                                                                                            <  4:276899/73‑1 (MQ=255)                                                                                                  ccTGCACTACCGCGAAACACCCCACTTCGTGCTGGCTCCGGAGTCCGGTGCC‑GCACGCCCGGTGCATTGCGGCAAACTGATGCTACTGGAGCGCCCACGGGCCTATGTGCACCTGCCCACCGCTTCGCTACAGCTGATCTATGACATgc                                                >  7:190269/1‑149 (MQ=255)                                                                                                  ccTGCACTACCGCGAAACACCCCACTTCGTGCTGGCTCCGGAGTCCGGTGCC‑GCACGCCCGGTGCATTGCGGCAAACTGATGCTACTGGAGCGCCCACGGGCCTATGTGCACCTGCCCACCGCTTCGCTACAGCTGATCTATGACATgc                                                >  2:58822/1‑149 (MQ=255)                                                                                                    tGCACTACCGCGAAACACCCCACTTCGTGCTGGCTCCGGAGTCCGGTGCC‑GCACGCCCGGTGCATTGCGGCAAACTGATGCTACTGGAGCGCCCACGGGCCTATGTGCACCTGCCCACCGCTTCGCTACAGCTGATCTATGACATgcgc                                              >  3:64291/1‑149 (MQ=255)                                                                                                      cACTACCGCGAAACACCCCACTTCGTGCTGGCTCCGGAGTCCGGTGCC‑GCACGCCCGGTGCATTGCGGCAAACTGATGCTACTGGAGCGCCCACGGGCCTATGTGCACCTGCCCACCGCTTCGCTACAGCTGATCTATGACATGCGCCt                                            >  5:327048/1‑149 (MQ=255)                                                                                                        cTACCGCGAAACACCCCACTTCGTGCTGGCTCCGGAGTCCGGTGCC‑GCACGCCCGGTGCATTGCGGCAAACTGATGCTACTGGAGCGCCCACGGGCCTATGTGCACCTGCCCACCGCTTCGCTACAGCTGATCTATGACATGCGCCTg                                           >  2:176403/1‑148 (MQ=255)                                                                                                         tACCGCGAAACACCCCACTTCGTGCTGGCTCCGGAGTCCGGTGCC‑GCACGCCCGGTGCATTGCGGCAAACTGATGCTACTGGAGCGCCCACGGGCCTATGTGCACCTGCCCACCGCTTCGCTACAGCTGATCTATGACATGCGCCTGc                                          >  8:112027/1‑148 (MQ=255)                                                                                                           ccGCGAAACACCCCACTTCGTGCTGGCTCCGGAGTCCGGTGCC‑GCACGCCCGGTGCATTGCGGCAAACTGATGCTACTGGAGCGCACACGGGCTTATGTGCACCTGCCCACCGCTTCGCTACAGCTGATCTATGACATGCGCCTGCaa                                        <  5:264068/148‑1 (MQ=255)                                                                                                            cgcgAAACACCCCACTTCGTGCTGGCTCCGGAGTCCGGTGCC‑GCACGCCCGGTGCATTGCGGCAAACTGATGCTACTGGAGCGCCCACGGGCCTATGTGCACCTGCCCACCGCTTCGCTACAGCTGATCTATGACATGCGCCTGCAAGt                                      >  3:188961/1‑149 (MQ=255)                                                                                                                 aaCACCCCACTTCGTGCTGGCTCCGGAGTCCGGTGCC‑GCACGCCCGGTGCATTGCGGCAAACTGATGCTACTGGAGCGCCCACGGGCCTATGTGCACCTGCCCACCGCTTCGCTACAGCTGATCTATGACATGCGCCTGCAAGTGcccc                                 <  4:64291/149‑1 (MQ=255)                                                                                                                     ccgcACTTCGTGCTGGCTCCGGAGTCCGGTGCC‑GCACGCCCGGTGCATTGCGGCAAACTGATGCTACTGGAGCGCCCACGGGCCTATGTGCACCTGCCCACCGCTTCGCTACAGCTGATCTATGACATGCGCCTGCAAGTGCCCCGGGa                             <  7:112027/146‑1 (MQ=255)                                                                                                                       ccACTTCGTGCTGGCTCCGGAGTCCGGTGCC‑GCACGCCCGGTGCATTGCGGCAAACTGATGCTACTGGAGCGCCCACGGGCCTATGTGCACCTGCCCACCGCTTCGCTACAGCTGATCTATGACATGCGCCTGCAAGTGCCCCgg                               >  4:55923/1‑145 (MQ=255)                                                                                                                       ccACTTCGTGCTGGCTCCGGAGTCCGGTGCC‑GCACGCCCGGTGCATTGCGGCAAACTGATGCTACTGGAGCGCCCACGGGCCTATGTGCACCTGCCCACCGCTTCGCTACAGCTGATCTATGACATGCGCCTGCAAGTGCCCCgg                               <  3:55923/145‑1 (MQ=255)                                                                                                                        cACTTCGTGCTGGCTCCGGAGTCCGGTGCC‑GCACGCCCGGTGCATTGCGGCAAACTGATGCTACTGGAGCGCCCACGGGCCTATGTGCACCTGCCCACCGCATCGCTACAGCTGATCTATGACATGCGCCTGCAAGTGCCCCGGGACg                           >  2:256254/1‑148 (MQ=255)                                                                                                                             cGTGCTGGCTCCGGAGTCCGGTGCC‑GCACGCCCGGTGCATTGCGGTAAACTGATGCTACTGGAGCGCCCACGGGCCTATGTGCACCTGCCCACCGCTTCGCTACAGCTGATCTATGACATGCGCCTGCAAGTGCCCCGGGACGCCaa                       <  4:297247/147‑1 (MQ=255)                                                                                                                               tGCTGGCTCCGGAGTCCGGTGCC‑GCACGCCCGGTGCATTGCGGCAAACTGATGCTACTGGAGCGCCCACGGGCCTATGTGCACCTGCCCACCGCTTCGCTACAGCTGATCTATGACATGCGCCTGCAAGTGCCCCGGGACGCCAAGaaa                   <  1:176403/149‑1 (MQ=255)                                                                                                                                 gcggCACCGGACTCCGGAGCCAGCACGCCCGGTGCATTGCGGCAAACTGATGCTACTGGAGCGCCCACGGGCCTATGTGCACCTGCCCACCGCTTCGCTACAGCTGATCTATGACATGCGCCTGCAAGTGCCCCGGGACGCCAAGAAAc                  >  2:274201/3‑149 (MQ=255)                                                                                                                                     cTCCGGAGTCCGGTGCC‑GCACGCCCGGTGCATTGCGACAAACTGATGCTACTGGAGCGCCCACGGGCCTATGTGCACCTGCCCACCGCTTCGCTACAGCTGATCTATGACATGCGCCTGCAAGTGCCCCGGGACGCCAAGAAACTCAGc             <  6:372898/149‑1 (MQ=255)                                                                                                                                      tCCGGAGTCCGGTGCC‑GCACGCCCGGTGCATTGCGGCAAACTGATGCTACTGGAGCGCCCACGGGCCTATGTGCACCTGCCCACCGCTTCGCTACAGCTGATCTATGACATGCGCCTGCAAGTGCCCCGGGACGCCAAGAACCTCAGCt            >  3:322314/1‑149 (MQ=255)                                                                                                                                      tCCGGAGTCCGGTGCC‑GCACGCCCGGTGCATTGCGGCAAACTGATGCTACTGGAGCGCCCACGGGCCTATGTGCACCTGCCCACCGCTTCGCTACAGCTGATCTATGACATGCGCCTGCAAGTGCCCCGGGACGCCAAGAAACTCAGCt            >  5:78535/1‑149 (MQ=255)                                                                                                                                        cGGAGTCCGGTGCC‑GCACGCCCGGTGCATTGCGGCAAACTGATGCTACTGGAGCGCCCACGGGCCTATGTGCACCTGCCCACCGCTTCGCTACAGCTGATCTATGACATGCGCCTGCAAGTGCCCCGGGACGCCAAGAAACTCAGCTTg          >  6:120423/1‑149 (MQ=255)                                                                                                                                        cGGAGTCCGGTGCC‑GCACGCCCGGTGCATTGCGGCAAACTGATGCTACTGGAGCGCCCACGGGCCTATGTGCACCTGCCCACCGCTTCGCTACAGCTGATCTATGACATGCGCCTGCAAGTGCCCCGGGACGCCAAGAAACTCAGCTTg          >  1:160280/1‑149 (MQ=255)                                                                                                                                           aGTCCGGTGCC‑GCACGCCCGGTGCATTGCGGCAAACTGATGCTACTGGAGCGCCCACGGGCCTATGTGCACCTGCCCACCGCTTCGCTACAGCTGATCTATGACATGCGCCTGCAAGTGCCCCGGGACGCCAAGAAACTCAGCTTGTTc       <  2:94057/149‑1 (MQ=255)                                                                                                                                             tCCGGTGCC‑GCACGCCCGGTGCATTGCGGCAAACTGATGCTACTGGAGCGCCCACGGGCCTATGTGCACCTGCCCACCGCTTCGCTACAGCTGATCTATGACATGCGCCTGCAAGTGCCCCGGGACGCCAAGAAACTCAGCTTGTTCCa     <  7:176918/149‑1 (MQ=255)                                                                                                                                               cGGTGCC‑GCACGCCCGGTGCATTGCGGCAAACTGATGCTACTGGAGCGCCCACGGGCCTATGTGCACCTGCCCACCGCTTCGCTACAGCTGATCTATGACATGCGCCTGCAAGTGCCCCGGGACGCCAAGAAACTCAGCTTGTTCCAc    <  6:327048/148‑1 (MQ=255)                                                                                                                                               cGGTGCC‑GCACGCCCGGTGCATTGCGGCAAACTGATGCTACTGGAGCGCCCACGGGCCTATGTGCACCTGCCCACCGCTTCGCTACAGCTGATCTATGACATGCGCCTGCAAGTGCCCCGGGACGCCAAGAAACTCAGCTTGTTCCACg   >  2:344745/1‑149 (MQ=255)                                                                                                                                                ggTGCC‑GCACGCCCGGTGCATTGCGGCAAACTGATGCTACTGGAGCGCCCACGGGCCTATGTGCACCTGCCCACCGCTTCGCTACAGCTGATCTATGACATGCGCCTGCAAGTGCCCCGGGACGCCAAGAAACTCAGCTTGTTCCACGt  <  5:120423/149‑1 (MQ=255)                                                                                                                                                ggTGCC‑GCACGCCCGGTGCATTGCGGCAAACTGATGCTACTGGAGCGCCCACGGGCCTATGTGCACCTGCCCACCGCTTCGCTACAGCTGATCTATGACATGCGCCTGCAAGTGCCCCGGGACGCCAAGAAACTCAGCTTGTTCCACGt  >  8:309494/1‑149 (MQ=255)                                                                                                                                                  |                                                                                                                                                    TACAGGAAGTGGCCGAGGAGTGCCTGCTGGAAACCCCGGAAGGTTGGCTGGGCGGGCGCTTCAACGACACCTGGCTGCCCATCCCCTACGCCGCCGCCCTGCACTACCGCGAAACACCCCACTTCGTGCTGGCTCCGGAGTCCGGTGCC‑GCACGCCCGGTGCATTGCGGCAAACTGATGCTACTGGAGCGCCCACGGGCCTATGTGCACCTGCCCACCGCTTCGCTACAGCTGATCTATGACATGCGCCTGCAAGTGCCCCGGGACGCCAAGAAACTCAGCTTGTTCCACGT  >  NC_002947/5613390‑5613681

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑