Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,623,970	T→C	9.0%	I221V (ATC→GTC)	PP_4942 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,623,970	0	T	C	9.0%	209.3 / 9.7	78	I221V (ATC→GTC)	PP_4942	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (36/34);  new base C (3/4);  total (40/38)
Fisher's exact test for biased strand distribution p-value = 7.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.10e-01

GTGACCGGGCGTCTTCCCAGGCGGCCTGGGTATTGAGGAAGCTCAGCACGCTGCCTGGGCTGAAGGCCTTGGTTTCAGGGTCGACGCCGCCATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTCGGCACCGGTGGCCGGGAACCCCCAGCGGCGCAGGAACAGGTCGCAATACATGCTGGCGATTTCCT  >  NC_002947/5623823‑5624116                                                                                                                                                    |                                                                                                                                                     gTGACCGGGCGTCTTCCCAGGCGGCCTGGGTATTGAGGAAGCTCAGCACGCTGCCTGGGCTGAAGGCCTTGGTTTCAGGGTCGACGCCGCCATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATg                                                                                                                                                     <  3:231295/149‑1 (MQ=255) gTGACCGGGCGTCTTCCCAGGCGGCCTGGGTATTGAGGAAGCTCAGCACGCTGCCTGGGCTGAAGGCCTTGGTTTCAGGGTCGACGCCGCCATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATg                                                                                                                                                     <  1:220197/149‑1 (MQ=255)      cGGGCGTCTTCCCATGCGGCCTGGGTATTGAGGAAGCTCAGCACGCTGCCTGGGCTGAAGGCCTTGGTTTCAGGGTCGACGCCGCCATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAAt                                                                                                                                                <  4:117051/149‑1 (MQ=255)           gTCTTCCCAGGCGGCCTGGGTATTGAGGAAGCTCAGCACGCTGCCTGGGCTGAAGGCCTTGGTTTCAGGGTCGACGCCGCCATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCt                                                                                                                                           <  6:178149/149‑1 (MQ=255)              tacccAGGCCGCCTGGGTATTGAGGAAGCTCAGCACGCTGCCTGGGCTGAAGGCCTTGGTTTCAGGGTCGACGCAGCCATTGAAGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCACCTGGATGGCAATCGGCTTAc                                                                                                                                        >  1:101117/3‑149 (MQ=255)               tCCCAGGAGGCCTGGGTATTGAGGAAGCTCAGCACGATCCCTGGGCTGAAGGCCTTGGTTTCAGGGACGACGCCGCCATTCATGTACTCGACGCTGATCCACTCCGGCGCTACGACGCGGTACACAAGCTGCATGGCAATAGGCTTAca                                                                                                                                       >  3:384534/1‑148 (MQ=255)                      atgcCTGGGTATTGAGGAATCTCAGCTGTCTTCCTGGTCTGAAGGCAGTGGTTGCAGGGGAGACGCCGACATTGATGTACTCTCCTCTGATAAACTCCTGCGGTTAGAGGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CTTCaagc                                                                                                                               <  4:384534/147‑1 (MQ=255)                        gcCTGGGTATTGAGGAAGCTCAGCACGCTGCCTGGGCTGAAGGCCTTGGTTTCAGGGTCGACGCCGCCATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGACGGTAAGCCGATTGC‑CATCcagctg                                                                                                                             <  6:288933/149‑3 (MQ=255)                        gcCTGGGTATTGAGGAAGCTCAGCACGCTGCCTGGGCTGAAGGCCTTGGTTTCAGGGTCGACGCCGCCATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGACGGTAAGCCGATTGC‑CATCcagctg                                                                                                                             <  6:160599/149‑3 (MQ=255)                         ccTGGGTATTGAGGAAGCTCAGCACGCTGCCTGGGCTGAAGGCCTTGGTTTCAGGGTCGACGCCGCCATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTACATGTCcagcac                                                                                                                             >  6:27478/1‑148 (MQ=255)                         ccTGGGTATTGAGGAAGCTCAGCACGCTGCCTGGGCTGAAGGCCTTGGTTTCAGGGTCGACGCCGCCATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGACGGTAAGCCGATTGC‑CATCcagctgg                                                                                                                            <  6:264573/149‑4 (MQ=255)                              gTATTGAGGAAGCTCAGCACGCTGCCTGGGCTGAAGGCCTTGGTTTCAGGGTCGACGCCGCCATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAacac                                                                                                                       >  4:174290/1‑149 (MQ=255)                               tATTGAGGAAGCTCAGCACGCTGCCTGGGCTGAAGGCCTTGGTTTCAGGGTCGACGCCGCCATTGATGTACTCGACGCTGATACACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACAcc                                                                                                                      >  8:235949/1‑149 (MQ=255)                                 ttGAGTAAGCTCCGCACGCTGCCTGGGCTGAAGGCCTTGGTTTCAGGGTCGACGCCGCCATGGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGa                                                                                                                    <  8:334436/149‑1 (MQ=255)                                    aGGAAGCTCAGCACGCTGCCTGGGCTGAAGGCCTTGGTTTCAGGGTCGACGCCGCCATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTa                                                                                                                                         >  4:168760/1‑126 (MQ=255)                                    aGGAAGCTCAGCACGCTGCCTGGGCTGAAGGCCTTGGTTTCAGGGTCGACGCCGCCATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTa                                                                                                                                         <  3:168760/126‑1 (MQ=255)                                    aGGAAGCTCAGCACGCTGCCTGGGCTGAAGGCCTTGGTTTCAGGGTCGACGCCGCCATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGcc                                                                                                                 >  4:87032/1‑149 (MQ=255)                                    aGGAAGCTCAGCACGCTGCCTGGGCTGAAGGCCTTGGTTTCAGGGTCGACGCCGCCATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGcc                                                                                                                 >  4:92528/1‑149 (MQ=255)                                        aGCTCAGCACGCTGCCTGGGCTGAAGGCCTTGGTTTCAGGGTCGACGCCGCCATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGAt                                                                                                              >  2:287566/1‑148 (MQ=255)                                         gCTCAGCACGCTGCCTGGGCTGAAGGCCTTGGTTTCAGGGTCGACGCCGCCATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATca                                                                                                            <  5:367705/149‑1 (MQ=255)                                           tCAGCACGCTGCCTGGGCTGAAGGCCTTGGTTTCAGGGTCGACGCCGCCATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATcag                                                                                                           >  6:388252/1‑148 (MQ=255)                                            cAGCACGCTGCCTGGGCTGAAGGCCTTGGTTTCAGGGTCGACGCCGCCATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATcag                                                                                                           >  1:316239/1‑147 (MQ=255)                                            cAGCACGCTGCCTGGGCTGAAGGCCTTGGTTTCAGGGTCGACGCCGCCATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATcag                                                                                                           >  1:27703/1‑147 (MQ=255)                                                      ccTGGGCTGAAGGCCTTGGTTTCAGGGTCGACGCCGCCATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGc                                                                                                 <  3:174290/147‑1 (MQ=255)                                                      ccTGGGCTGAAGGCCTTGGTTTCAGGGTCGACGCCGCCATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAg                                                                                               <  3:185144/149‑1 (MQ=255)                                                            cTGAAGGCCTTGGTTTCAGGGTCGACGCCGCCATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTc                                                                                        >  8:186584/1‑149 (MQ=255)                                                            cTGAAGGCCTTGGTTTCAGGGTCGACGCCGCCATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTc                                                                                        <  2:316239/149‑1 (MQ=255)                                                               aaGGCCTTGTTTTCTGTGTCGAGTCCGGCATTTATGTACTCGTCGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAG‑CGTCCAGCATCAACACCGAGCCGATca                                                                                                            <  5:220745/127‑1 (MQ=255)                                                               aaGGCCTTGGTTTCAGGGTCGACGCCGCCATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCaa                                                                                     <  3:92528/149‑1 (MQ=255)                                                               aaGGCCTTGGTTTCAGGGTCGACGCCGCCATTGATGTACTCGACGCTGATCCACTCAGGCGCTTCGACGCGGTACACCAGCTGGATGGCAAACGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATca                                                                                                            >  6:220745/1‑127 (MQ=255)                                                                aGGCCTTGGTTTCAGGGTCGACGCCGCCATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCaa                                                                                     <  3:87032/148‑1 (MQ=255)                                                                aGGCCTTGGTTTCAGGGTCGACGCCGCCATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAac                                                                                    <  5:388252/149‑1 (MQ=255)                                                                    cTTGGTTTCAGGGTCGACGCCGCCATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAAcaca                                                                                 >  8:150455/1‑148 (MQ=255)                                                                    cTTGGTTTCAGGGTCGACGCCGCCATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACAt                                                                                <  7:150455/149‑1 (MQ=255)                                                                    cTTGGTTTCAGGGTCGACGCCGCCATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACAt                                                                                >  6:92238/1‑149 (MQ=255)                                                                     ttGGTTTCAGGGTCGACGCCGCCATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATc                                                                               >  1:213904/1‑149 (MQ=255)                                                                       ggTTTCAGGGTCGACGCCGCCATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCgg                                                                             >  6:300550/1‑149 (MQ=255)                                                                         tttCAGGGTCGACGCCGCCATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGc                                                                            >  2:314918/1‑148 (MQ=255)                                                                          ttCAGGGTCGACGCCGCCATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAac                                                                                    <  2:184714/139‑1 (MQ=255)                                                                          ttCAGGGTCGACGCCGCCATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAac                                                                                    >  1:184714/1‑139 (MQ=255)                                                                               ggTCGACGCCGCCATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACCCATCGGCCATACGc                                                                     >  2:315196/1‑149 (MQ=255)                                                                               ggTCGACGCCGCCATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGc                                                                     >  3:399996/1‑149 (MQ=255)                                                                                  cGACGCCGCCATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGAAGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTCg                                                                  >  1:4128/1‑149 (MQ=255)                                                                                   gACGCCGCCATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTCgg                                                                 <  4:399996/149‑1 (MQ=255)                                                                                   gACGCCGCCATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTCg                                                                  <  7:186584/148‑1 (MQ=255)                                                                                         gccATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTCGGCACCgg                                                           >  2:390428/1‑149 (MQ=255)                                                                                                    aCTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTc                                                                                        >  7:81328/1‑109 (MQ=255)                                                                                                    aCTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTc                                                                                        <  8:81328/109‑1 (MQ=255)                                                                                                     cTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTCGGCACCGGTGGCCGGGAccc                                               >  4:143911/1‑149 (MQ=255)                                                                                                         aCGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTCGGCACCGGTGGCCGGGAACCCCCa                                           <  1:196672/149‑1 (MQ=255)                                                                                                          cGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CTTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTCGGCACCGGTGGCCGGGAACCCCCAg                                          <  7:235949/149‑1 (MQ=255)                                                                                                          cGCTGATCCACTCCGGCGCTTCGAAGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTCGGCACCGGTGGCCGGGAACCCCCAg                                          <  8:143131/149‑1 (MQ=255)                                                                                                           gCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTCGGCACCGGTGGCCGGGAACCCCCAgc                                         >  4:258489/1‑149 (MQ=255)                                                                                                              gATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTCGGCACCGGTGGCCGGGAACCCCCAgcggc                                      >  6:173853/1‑149 (MQ=255)                                                                                                                  cACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGCTGCTCCAACACATCGGCCATACGCTCGGCACCGGTGGCCGGGAACCCCCAGCGgcgc                                    <  1:314918/148‑1 (MQ=255)                                                                                                                  cACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTCGGCACCGGTGGCCGGGAACCCCCAGCGGCGCAg                                  >  6:346924/1‑149 (MQ=255)                                                                                                                   aCTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTCGGCACCGGTGGCCGGGAACCCCCAGCGGCGCAgg                                 <  5:92238/149‑1 (MQ=255)                                                                                                                   aCTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTCGGCACCGGTGGCCGGGAACCCCCAGCGGCGCAg                                  >  1:86480/1‑148 (MQ=255)                                                                                                                       cGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTCGGCACCGGTGGCCg                                                     <  3:306399/125‑1 (MQ=255)                                                                                                                       cGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTCGGCACCGGTGGCCg                                                     >  4:306399/1‑125 (MQ=255)                                                                                                                        ggCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTCGGCACCGGTGGCCGGGAACCCCCAGCGGCGCAGGAACAg                            <  2:27703/149‑1 (MQ=255)                                                                                                                         gcgcTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTCGGCACCGGTGGCCGGGAACCCCCAGCGGCGCAGGAACAgg                           <  5:300550/149‑1 (MQ=255)                                                                                                                             ttCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTCGGCACCGGTGGCCGGGAACCCCCAGCGGCGCAGGAACAGGTCg                        >  3:239297/1‑148 (MQ=255)                                                                                                                                gACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCTACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTCGGCACCGGAGGCCGGGAACCCCCAGCGGCGCAGGAACAGGTCGCAAt                    <  3:226216/149‑1 (MQ=255)                                                                                                                                gACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTCGGCACCGGTGGCCGGGAACCCCCAGCGGCGCAGGAACAGGTCGCAAt                    <  2:213904/149‑1 (MQ=255)                                                                                                                                gACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTCCGCACCGGTGGCCGGGAACCCCCAGCGGCGCAGGAACAGGTCGCat                     <  1:287566/148‑2 (MQ=255)                                                                                                                                    cgGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTCGGCACCGGTGGCCGGGAACCCCCAGCGGCGCAGGAACAGGTCGCAATACAt                <  2:86480/149‑1 (MQ=255)                                                                                                                                     ggTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTCGGCACCGGTGGCCGGGAACCCCCAGCGGCGCAGGAACAGGTCGCAATACATg               >  1:239520/1‑149 (MQ=255)                                                                                                                                      gTAGACCAGCTGGATGGCAATCCGCTTAC‑CGTCCAGCAGGAACACCGAGCCGATCAGCAGCTCTCGCAGGC‑GCTCCAACACATCGGCCATACGCTCGGCACCGGTGGCCGGGAACCCCCAGCGGCGCAGGAACAGGTCGCAATACATGc              <  2:101117/149‑1 (MQ=255)                                                                                                                                        gttgctgCTGGACGGTAAGCCGATTGC‑CATCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTCGGCACCGGTGGCCGGGAACCCCCAGCGGCGCAGGAACAGGTCGCAATACATGCTg            >  5:167847/7‑149 (MQ=38)                                                                                                                                          acCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTCGGCACCGGTGGCCGGGAACCCCCAGCGGCGCa                                   >  2:229116/1‑124 (MQ=255)                                                                                                                                          acCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTCGGCACCGGTGGCCGGGAACCCCCAGCGGCGCAGGAACAGGTCGCAATACATGCTGGc          >  3:99406/1‑149 (MQ=255)                                                                                                                                             tgCTGGACGGTAAGCCGATTGC‑CATCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTCGGCACCGGTGGCCGGGAACCCCCAGCGGCGCAGGAACAGGTc                         <  7:279653/130‑1 (MQ=255)                                                                                                                                             tgCTGGACGGTAAGCCGATTGC‑CATCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTCGGCACCGGTGGCCGGGAACCCCCAGCGGCGCAGGAACAGGTc                         >  8:279653/2‑131 (MQ=255)                                                                                                                                             tgCTGGACGGTAAGCCGATTGC‑CATCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTCGGCACCGGTGGCCGGGAACCCCCAGCGGCGCAGGAACAGGTCGCAATACATGCTGGCGAt       >  7:71859/2‑149 (MQ=255)                                                                                                                                              gCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTCGGCACCGGTGGCCGGGAACCCCCAGCGGCGCAGGAACAGGTCGCAATACATGCTGGCGAtt      >  8:192671/1‑149 (MQ=255)                                                                                                                                              gCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTCGGCACCGGTGGCCGGGAACCCCCAGCGGCGCAGGAACAGGTCGCAATACATGCTGGCGAtt      <  5:173853/149‑1 (MQ=255)                                                                                                                                                  gATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTCGGCACCGGTGGCCGGGAACCCCCAGCGGCGCAGGAACAGGTCGCAATACATGCTGGCGATTTCCt  >  8:338060/1‑149 (MQ=255)                                                                                                                                                    |                                                                                                                                                     GTGACCGGGCGTCTTCCCAGGCGGCCTGGGTATTGAGGAAGCTCAGCACGCTGCCTGGGCTGAAGGCCTTGGTTTCAGGGTCGACGCCGCCATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTCGGCACCGGTGGCCGGGAACCCCCAGCGGCGCAGGAACAGGTCGCAATACATGCTGGCGATTTCCT  >  NC_002947/5623823‑5624116

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑