Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,623,980	C→A	8.3%	K217N (AAG→AAT)	PP_4942 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,623,980	0	C	A	8.3%	254.3 / 9.3	80	K217N (AAG→AAT)	PP_4942	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (40/33);  new base A (3/4);  total (43/37)
Fisher's exact test for biased strand distribution p-value = 6.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.01e-01

GTCTTCCCAGGCGGCCTGGGTATTGAGGAAGCTCAGCACGCTGCCTGGGCTGAAGGCCTTGGTTTCAGGGTCGACGCCGCCATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTCGGCACCGGTGGCCGGGAACCCCCAGCGGCGCAGGAACAGGTCGCAATACATGCTGGCGATTTCCTGGGCACTGAAG  >  NC_002947/5623833‑5624127                                                                                                                                                    |                                                                                                                                                      gTCTTCCCAGGCGGCCTGGGTATTGAGGAAGCTCAGCACGCTGCCTGGGCTGAAGGCCTTGGTTTCAGGGTCGACGCCGCCATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCt                                                                                                                                                      <  6:178149/149‑1 (MQ=255)    tacccAGGCCGCCTGGGTATTGAGGAAGCTCAGCACGCTGCCTGGGCTGAAGGCCTTGGTTTCAGGGTCGACGCAGCCATTGAAGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCACCTGGATGGCAATCGGCTTAc                                                                                                                                                   >  1:101117/3‑149 (MQ=255)     tCCCAGGAGGCCTGGGTATTGAGGAAGCTCAGCACGATCCCTGGGCTGAAGGCCTTGGTTTCAGGGACGACGCCGCCATTCATGTACTCGACGCTGATCCACTCCGGCGCTACGACGCGGTACACAAGCTGCATGGCAATAGGCTTAca                                                                                                                                                  >  3:384534/1‑148 (MQ=255)            atgcCTGGGTATTGAGGAATCTCAGCTGTCTTCCTGGTCTGAAGGCAGTGGTTGCAGGGGAGACGCCGACATTGATGTACTCTCCTCTGATAAACTCCTGCGGTTAGAGGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CTTCaagc                                                                                                                                          <  4:384534/147‑1 (MQ=255)              gcCTGGGTATTGAGGAAGCTCAGCACGCTGCCTGGGCTGAAGGCCTTGGTTTCAGGGTCGACGCCGCCATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGACGGTAAGCCGATTGC‑CATCcagctg                                                                                                                                        <  6:160599/149‑3 (MQ=255)              gcCTGGGTATTGAGGAAGCTCAGCACGCTGCCTGGGCTGAAGGCCTTGGTTTCAGGGTCGACGCCGCCATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGACGGTAAGCCGATTGC‑CATCcagctg                                                                                                                                        <  6:288933/149‑3 (MQ=255)               ccTGGGTATTGAGGAAGCTCAGCACGCTGCCTGGGCTGAAGGCCTTGGTTTCAGGGTCGACGCCGCCATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTACATGTCcagcac                                                                                                                                        >  6:27478/1‑148 (MQ=255)               ccTGGGTATTGAGGAAGCTCAGCACGCTGCCTGGGCTGAAGGCCTTGGTTTCAGGGTCGACGCCGCCATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGACGGTAAGCCGATTGC‑CATCcagctgg                                                                                                                                       <  6:264573/149‑4 (MQ=255)                    gTATTGAGGAAGCTCAGCACGCTGCCTGGGCTGAAGGCCTTGGTTTCAGGGTCGACGCCGCCATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAacac                                                                                                                                  >  4:174290/1‑149 (MQ=255)                     tATTGAGGAAGCTCAGCACGCTGCCTGGGCTGAAGGCCTTGGTTTCAGGGTCGACGCCGCCATTGATGTACTCGACGCTGATACACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACAcc                                                                                                                                 >  8:235949/1‑149 (MQ=255)                       ttGAGTAAGCTCCGCACGCTGCCTGGGCTGAAGGCCTTGGTTTCAGGGTCGACGCCGCCATGGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGa                                                                                                                               <  8:334436/149‑1 (MQ=255)                          aGGAAGCTCAGCACGCTGCCTGGGCTGAAGGCCTTGGTTTCAGGGTCGACGCCGCCATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTa                                                                                                                                                    >  4:168760/1‑126 (MQ=255)                          aGGAAGCTCAGCACGCTGCCTGGGCTGAAGGCCTTGGTTTCAGGGTCGACGCCGCCATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTa                                                                                                                                                    <  3:168760/126‑1 (MQ=255)                          aGGAAGCTCAGCACGCTGCCTGGGCTGAAGGCCTTGGTTTCAGGGTCGACGCCGCCATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGcc                                                                                                                            >  4:92528/1‑149 (MQ=255)                          aGGAAGCTCAGCACGCTGCCTGGGCTGAAGGCCTTGGTTTCAGGGTCGACGCCGCCATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGcc                                                                                                                            >  4:87032/1‑149 (MQ=255)                              aGCTCAGCACGCTGCCTGGGCTGAAGGCCTTGGTTTCAGGGTCGACGCCGCCATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGAt                                                                                                                         >  2:287566/1‑148 (MQ=255)                               gCTCAGCACGCTGCCTGGGCTGAAGGCCTTGGTTTCAGGGTCGACGCCGCCATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATca                                                                                                                       <  5:367705/149‑1 (MQ=255)                                 tCAGCACGCTGCCTGGGCTGAAGGCCTTGGTTTCAGGGTCGACGCCGCCATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATcag                                                                                                                      >  6:388252/1‑148 (MQ=255)                                  cAGCACGCTGCCTGGGCTGAAGGCCTTGGTTTCAGGGTCGACGCCGCCATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATcag                                                                                                                      >  1:316239/1‑147 (MQ=255)                                  cAGCACGCTGCCTGGGCTGAAGGCCTTGGTTTCAGGGTCGACGCCGCCATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATcag                                                                                                                      >  1:27703/1‑147 (MQ=255)                                            ccTGGGCTGAAGGCCTTGGTTTCAGGGTCGACGCCGCCATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGc                                                                                                            <  3:174290/147‑1 (MQ=255)                                            ccTGGGCTGAAGGCCTTGGTTTCAGGGTCGACGCCGCCATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAg                                                                                                          <  3:185144/149‑1 (MQ=255)                                                  cTGAAGGCCTTGGTTTCAGGGTCGACGCCGCCATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTc                                                                                                   >  8:186584/1‑149 (MQ=255)                                                  cTGAAGGCCTTGGTTTCAGGGTCGACGCCGCCATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTc                                                                                                   <  2:316239/149‑1 (MQ=255)                                                     aaGGCCTTGTTTTCTGTGTCGAGTCCGGCATTTATGTACTCGTCGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAG‑CGTCCAGCATCAACACCGAGCCGATca                                                                                                                       <  5:220745/127‑1 (MQ=255)                                                     aaGGCCTTGGTTTCAGGGTCGACGCCGCCATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCaa                                                                                                <  3:92528/149‑1 (MQ=255)                                                     aaGGCCTTGGTTTCAGGGTCGACGCCGCCATTGATGTACTCGACGCTGATCCACTCAGGCGCTTCGACGCGGTACACCAGCTGGATGGCAAACGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATca                                                                                                                       >  6:220745/1‑127 (MQ=255)                                                      aGGCCTTGGTTTCAGGGTCGACGCCGCCATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCaa                                                                                                <  3:87032/148‑1 (MQ=255)                                                      aGGCCTTGGTTTCAGGGTCGACGCCGCCATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAac                                                                                               <  5:388252/149‑1 (MQ=255)                                                          cTTGGTTTCAGGGTCGACGCCGCCATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAAcaca                                                                                            >  8:150455/1‑148 (MQ=255)                                                          cTTGGTTTCAGGGTCGACGCCGCCATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACAt                                                                                           >  6:92238/1‑149 (MQ=255)                                                          cTTGGTTTCAGGGTCGACGCCGCCATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACAt                                                                                           <  7:150455/149‑1 (MQ=255)                                                           ttGGTTTCAGGGTCGACGCCGCCATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATc                                                                                          >  1:213904/1‑149 (MQ=255)                                                             ggTTTCAGGGTCGACGCCGCCATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCgg                                                                                        >  6:300550/1‑149 (MQ=255)                                                               tttCAGGGTCGACGCCGCCATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGc                                                                                       >  2:314918/1‑148 (MQ=255)                                                                ttCAGGGTCGACGCCGCCATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAac                                                                                               >  1:184714/1‑139 (MQ=255)                                                                ttCAGGGTCGACGCCGCCATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAac                                                                                               <  2:184714/139‑1 (MQ=255)                                                                     ggTCGACGCCGCCATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACCCATCGGCCATACGc                                                                                >  2:315196/1‑149 (MQ=255)                                                                     ggTCGACGCCGCCATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGc                                                                                >  3:399996/1‑149 (MQ=255)                                                                        cGACGCCGCCATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGAAGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTCg                                                                             >  1:4128/1‑149 (MQ=255)                                                                         gACGCCGCCATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTCgg                                                                            <  4:399996/149‑1 (MQ=255)                                                                         gACGCCGCCATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTCg                                                                             <  7:186584/148‑1 (MQ=255)                                                                               gccATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTCGGCACCgg                                                                      >  2:390428/1‑149 (MQ=255)                                                                                          aCTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTc                                                                                                   >  7:81328/1‑109 (MQ=255)                                                                                          aCTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTc                                                                                                   <  8:81328/109‑1 (MQ=255)                                                                                           cTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTCGGCACCGGTGGCCGGGAccc                                                          >  4:143911/1‑149 (MQ=255)                                                                                               aCGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTCGGCACCGGTGGCCGGGAACCCCCa                                                      <  1:196672/149‑1 (MQ=255)                                                                                                cGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CTTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTCGGCACCGGTGGCCGGGAACCCCCAg                                                     <  7:235949/149‑1 (MQ=255)                                                                                                cGCTGATCCACTCCGGCGCTTCGAAGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTCGGCACCGGTGGCCGGGAACCCCCAg                                                     <  8:143131/149‑1 (MQ=255)                                                                                                 gCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTCGGCACCGGTGGCCGGGAACCCCCAgc                                                    >  4:258489/1‑149 (MQ=255)                                                                                                    gATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTCGGCACCGGTGGCCGGGAACCCCCAgcggc                                                 >  6:173853/1‑149 (MQ=255)                                                                                                        cACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGCTGCTCCAACACATCGGCCATACGCTCGGCACCGGTGGCCGGGAACCCCCAGCGgcgc                                               <  1:314918/148‑1 (MQ=255)                                                                                                        cACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTCGGCACCGGTGGCCGGGAACCCCCAGCGGCGCAg                                             >  6:346924/1‑149 (MQ=255)                                                                                                         aCTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTCGGCACCGGTGGCCGGGAACCCCCAGCGGCGCAgg                                            <  5:92238/149‑1 (MQ=255)                                                                                                         aCTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTCGGCACCGGTGGCCGGGAACCCCCAGCGGCGCAg                                             >  1:86480/1‑148 (MQ=255)                                                                                                             cGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTCGGCACCGGTGGCCg                                                                >  4:306399/1‑125 (MQ=255)                                                                                                             cGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTCGGCACCGGTGGCCg                                                                <  3:306399/125‑1 (MQ=255)                                                                                                              ggCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTCGGCACCGGTGGCCGGGAACCCCCAGCGGCGCAGGAACAg                                       <  2:27703/149‑1 (MQ=255)                                                                                                               gcgcTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTCGGCACCGGTGGCCGGGAACCCCCAGCGGCGCAGGAACAgg                                      <  5:300550/149‑1 (MQ=255)                                                                                                                   ttCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTCGGCACCGGTGGCCGGGAACCCCCAGCGGCGCAGGAACAGGTCg                                   >  3:239297/1‑148 (MQ=255)                                                                                                                      gACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCTACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTCGGCACCGGAGGCCGGGAACCCCCAGCGGCGCAGGAACAGGTCGCAAt                               <  3:226216/149‑1 (MQ=255)                                                                                                                      gACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTCGGCACCGGTGGCCGGGAACCCCCAGCGGCGCAGGAACAGGTCGCAAt                               <  2:213904/149‑1 (MQ=255)                                                                                                                      gACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTCCGCACCGGTGGCCGGGAACCCCCAGCGGCGCAGGAACAGGTCGCat                                <  1:287566/148‑2 (MQ=255)                                                                                                                          cgGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTCGGCACCGGTGGCCGGGAACCCCCAGCGGCGCAGGAACAGGTCGCAATACAt                           <  2:86480/149‑1 (MQ=255)                                                                                                                           ggTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTCGGCACCGGTGGCCGGGAACCCCCAGCGGCGCAGGAACAGGTCGCAATACATg                          >  1:239520/1‑149 (MQ=255)                                                                                                                            gTAGACCAGCTGGATGGCAATCCGCTTAC‑CGTCCAGCAGGAACACCGAGCCGATCAGCAGCTCTCGCAGGC‑GCTCCAACACATCGGCCATACGCTCGGCACCGGTGGCCGGGAACCCCCAGCGGCGCAGGAACAGGTCGCAATACATGc                         <  2:101117/149‑1 (MQ=255)                                                                                                                              gttgctgCTGGACGGTAAGCCGATTGC‑CATCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTCGGCACCGGTGGCCGGGAACCCCCAGCGGCGCAGGAACAGGTCGCAATACATGCTg                       >  5:167847/7‑149 (MQ=38)                                                                                                                                acCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTCGGCACCGGTGGCCGGGAACCCCCAGCGGCGCa                                              >  2:229116/1‑124 (MQ=255)                                                                                                                                acCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTCGGCACCGGTGGCCGGGAACCCCCAGCGGCGCAGGAACAGGTCGCAATACATGCTGGc                     >  3:99406/1‑149 (MQ=255)                                                                                                                                   tgCTGGACGGTAAGCCGATTGC‑CATCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTCGGCACCGGTGGCCGGGAACCCCCAGCGGCGCAGGAACAGGTc                                    <  7:279653/130‑1 (MQ=255)                                                                                                                                   tgCTGGACGGTAAGCCGATTGC‑CATCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTCGGCACCGGTGGCCGGGAACCCCCAGCGGCGCAGGAACAGGTc                                    >  8:279653/2‑131 (MQ=255)                                                                                                                                   tgCTGGACGGTAAGCCGATTGC‑CATCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTCGGCACCGGTGGCCGGGAACCCCCAGCGGCGCAGGAACAGGTCGCAATACATGCTGGCGAt                  >  7:71859/2‑149 (MQ=255)                                                                                                                                    gCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTCGGCACCGGTGGCCGGGAACCCCCAGCGGCGCAGGAACAGGTCGCAATACATGCTGGCGAtt                 <  5:173853/149‑1 (MQ=255)                                                                                                                                    gCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTCGGCACCGGTGGCCGGGAACCCCCAGCGGCGCAGGAACAGGTCGCAATACATGCTGGCGAtt                 >  8:192671/1‑149 (MQ=255)                                                                                                                                        gATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTCGGCACCGGTGGCCGGGAACCCCCAGCGGCGCAGGAACAGGTCGCAATACATGCTGGCGATTTCCt             >  8:338060/1‑149 (MQ=255)                                                                                                                                           ggCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTc                                                                              <  5:330751/81‑1 (MQ=255)                                                                                                                                           ggCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTc                                                                              >  6:330751/1‑81 (MQ=255)                                                                                                                                            gCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTCGGCACCGGTGGCCGGGAACCCCCAGCGGCGCAGGAACAGGTCGCAATACATGCTGGCGATTTCCTggg          <  4:239297/148‑1 (MQ=255)                                                                                                                                            gCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTCGGCACCGGTGGCCGGGAACCCCCAGCGGCGCAGGAACAGGTCGCAATACATGCTGGCGATTTCCTGGGc         >  2:179111/1‑149 (MQ=255)                                                                                                                                                   gCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTCGGCACCGGTGGCCGGGAACCCCCCGCGGCGCAGGAACAGGTCGCAATACATGCTGGCGATTTCCTGGGCACTGAAg  >  2:258848/1‑149 (MQ=255)                                                                                                                                                    |                                                                                                                                                      GTCTTCCCAGGCGGCCTGGGTATTGAGGAAGCTCAGCACGCTGCCTGGGCTGAAGGCCTTGGTTTCAGGGTCGACGCCGCCATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTCGGCACCGGTGGCCGGGAACCCCCAGCGGCGCAGGAACAGGTCGCAATACATGCTGGCGATTTCCTGGGCACTGAAG  >  NC_002947/5623833‑5624127

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑