Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,623,983	A→G	8.9%	G216G (GGT→GGC)	PP_4942 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,623,983	0	A	G	8.9%	208.9 / 9.0	78	G216G (GGT→GGC)	PP_4942	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (39/32);  new base G (3/4);  total (42/36)
Fisher's exact test for biased strand distribution p-value = 6.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.77e-01

TTCCCAGGCGGCCTGGGTATTGAGGAAGCTCAGCACGCTGCCTGGGCTGAAGGCCTTGGTTTCAGGGTCGACGCCGCCATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTCGGCACCGGTGGCCGGGAACCCCCAGCGGCGCAGGAACAGGTCGCAATACATGCTGGCGATTTCCTGGGCACTGAAG  >  NC_002947/5623836‑5624127                                                                                                                                                    |                                                                                                                                                   tacccAGGCCGCCTGGGTATTGAGGAAGCTCAGCACGCTGCCTGGGCTGAAGGCCTTGGTTTCAGGGTCGACGCAGCCATTGAAGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCACCTGGATGGCAATCGGCTTAc                                                                                                                                                   >  1:101117/3‑149 (MQ=255)  tCCCAGGAGGCCTGGGTATTGAGGAAGCTCAGCACGATCCCTGGGCTGAAGGCCTTGGTTTCAGGGACGACGCCGCCATTCATGTACTCGACGCTGATCCACTCCGGCGCTACGACGCGGTACACAAGCTGCATGGCAATAGGCTTAca                                                                                                                                                  >  3:384534/1‑148 (MQ=255)         atgcCTGGGTATTGAGGAATCTCAGCTGTCTTCCTGGTCTGAAGGCAGTGGTTGCAGGGGAGACGCCGACATTGATGTACTCTCCTCTGATAAACTCCTGCGGTTAGAGGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CTTCaagc                                                                                                                                          <  4:384534/147‑1 (MQ=255)           gcCTGGGTATTGAGGAAGCTCAGCACGCTGCCTGGGCTGAAGGCCTTGGTTTCAGGGTCGACGCCGCCATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGACGGTAAGCCGATTGC‑CATCcagctg                                                                                                                                        <  6:160599/149‑3 (MQ=255)           gcCTGGGTATTGAGGAAGCTCAGCACGCTGCCTGGGCTGAAGGCCTTGGTTTCAGGGTCGACGCCGCCATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGACGGTAAGCCGATTGC‑CATCcagctg                                                                                                                                        <  6:288933/149‑3 (MQ=255)            ccTGGGTATTGAGGAAGCTCAGCACGCTGCCTGGGCTGAAGGCCTTGGTTTCAGGGTCGACGCCGCCATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTACATGTCcagcac                                                                                                                                        >  6:27478/1‑148 (MQ=255)            ccTGGGTATTGAGGAAGCTCAGCACGCTGCCTGGGCTGAAGGCCTTGGTTTCAGGGTCGACGCCGCCATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGACGGTAAGCCGATTGC‑CATCcagctgg                                                                                                                                       <  6:264573/149‑4 (MQ=255)                 gTATTGAGGAAGCTCAGCACGCTGCCTGGGCTGAAGGCCTTGGTTTCAGGGTCGACGCCGCCATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAacac                                                                                                                                  >  4:174290/1‑149 (MQ=255)                  tATTGAGGAAGCTCAGCACGCTGCCTGGGCTGAAGGCCTTGGTTTCAGGGTCGACGCCGCCATTGATGTACTCGACGCTGATACACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACAcc                                                                                                                                 >  8:235949/1‑149 (MQ=255)                    ttGAGTAAGCTCCGCACGCTGCCTGGGCTGAAGGCCTTGGTTTCAGGGTCGACGCCGCCATGGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGa                                                                                                                               <  8:334436/149‑1 (MQ=255)                       aGGAAGCTCAGCACGCTGCCTGGGCTGAAGGCCTTGGTTTCAGGGTCGACGCCGCCATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTa                                                                                                                                                    <  3:168760/126‑1 (MQ=255)                       aGGAAGCTCAGCACGCTGCCTGGGCTGAAGGCCTTGGTTTCAGGGTCGACGCCGCCATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTa                                                                                                                                                    >  4:168760/1‑126 (MQ=255)                       aGGAAGCTCAGCACGCTGCCTGGGCTGAAGGCCTTGGTTTCAGGGTCGACGCCGCCATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGcc                                                                                                                            >  4:87032/1‑149 (MQ=255)                       aGGAAGCTCAGCACGCTGCCTGGGCTGAAGGCCTTGGTTTCAGGGTCGACGCCGCCATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGcc                                                                                                                            >  4:92528/1‑149 (MQ=255)                           aGCTCAGCACGCTGCCTGGGCTGAAGGCCTTGGTTTCAGGGTCGACGCCGCCATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGAt                                                                                                                         >  2:287566/1‑148 (MQ=255)                            gCTCAGCACGCTGCCTGGGCTGAAGGCCTTGGTTTCAGGGTCGACGCCGCCATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATca                                                                                                                       <  5:367705/149‑1 (MQ=255)                              tCAGCACGCTGCCTGGGCTGAAGGCCTTGGTTTCAGGGTCGACGCCGCCATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATcag                                                                                                                      >  6:388252/1‑148 (MQ=255)                               cAGCACGCTGCCTGGGCTGAAGGCCTTGGTTTCAGGGTCGACGCCGCCATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATcag                                                                                                                      >  1:27703/1‑147 (MQ=255)                               cAGCACGCTGCCTGGGCTGAAGGCCTTGGTTTCAGGGTCGACGCCGCCATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATcag                                                                                                                      >  1:316239/1‑147 (MQ=255)                                         ccTGGGCTGAAGGCCTTGGTTTCAGGGTCGACGCCGCCATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGc                                                                                                            <  3:174290/147‑1 (MQ=255)                                         ccTGGGCTGAAGGCCTTGGTTTCAGGGTCGACGCCGCCATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAg                                                                                                          <  3:185144/149‑1 (MQ=255)                                               cTGAAGGCCTTGGTTTCAGGGTCGACGCCGCCATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTc                                                                                                   <  2:316239/149‑1 (MQ=255)                                               cTGAAGGCCTTGGTTTCAGGGTCGACGCCGCCATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTc                                                                                                   >  8:186584/1‑149 (MQ=255)                                                  aaGGCCTTGTTTTCTGTGTCGAGTCCGGCATTTATGTACTCGTCGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAG‑CGTCCAGCATCAACACCGAGCCGATca                                                                                                                       <  5:220745/127‑1 (MQ=255)                                                  aaGGCCTTGGTTTCAGGGTCGACGCCGCCATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCaa                                                                                                <  3:92528/149‑1 (MQ=255)                                                  aaGGCCTTGGTTTCAGGGTCGACGCCGCCATTGATGTACTCGACGCTGATCCACTCAGGCGCTTCGACGCGGTACACCAGCTGGATGGCAAACGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATca                                                                                                                       >  6:220745/1‑127 (MQ=255)                                                   aGGCCTTGGTTTCAGGGTCGACGCCGCCATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCaa                                                                                                <  3:87032/148‑1 (MQ=255)                                                   aGGCCTTGGTTTCAGGGTCGACGCCGCCATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAac                                                                                               <  5:388252/149‑1 (MQ=255)                                                       cTTGGTTTCAGGGTCGACGCCGCCATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAAcaca                                                                                            >  8:150455/1‑148 (MQ=255)                                                       cTTGGTTTCAGGGTCGACGCCGCCATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACAt                                                                                           <  7:150455/149‑1 (MQ=255)                                                       cTTGGTTTCAGGGTCGACGCCGCCATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACAt                                                                                           >  6:92238/1‑149 (MQ=255)                                                        ttGGTTTCAGGGTCGACGCCGCCATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATc                                                                                          >  1:213904/1‑149 (MQ=255)                                                          ggTTTCAGGGTCGACGCCGCCATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCgg                                                                                        >  6:300550/1‑149 (MQ=255)                                                            tttCAGGGTCGACGCCGCCATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGc                                                                                       >  2:314918/1‑148 (MQ=255)                                                             ttCAGGGTCGACGCCGCCATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAac                                                                                               >  1:184714/1‑139 (MQ=255)                                                             ttCAGGGTCGACGCCGCCATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAac                                                                                               <  2:184714/139‑1 (MQ=255)                                                                  ggTCGACGCCGCCATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACCCATCGGCCATACGc                                                                                >  2:315196/1‑149 (MQ=255)                                                                  ggTCGACGCCGCCATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGc                                                                                >  3:399996/1‑149 (MQ=255)                                                                     cGACGCCGCCATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGAAGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTCg                                                                             >  1:4128/1‑149 (MQ=255)                                                                      gACGCCGCCATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTCgg                                                                            <  4:399996/149‑1 (MQ=255)                                                                      gACGCCGCCATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTCg                                                                             <  7:186584/148‑1 (MQ=255)                                                                            gccATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTCGGCACCgg                                                                      >  2:390428/1‑149 (MQ=255)                                                                                       aCTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTc                                                                                                   >  7:81328/1‑109 (MQ=255)                                                                                       aCTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTc                                                                                                   <  8:81328/109‑1 (MQ=255)                                                                                        cTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTCGGCACCGGTGGCCGGGAccc                                                          >  4:143911/1‑149 (MQ=255)                                                                                            aCGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTCGGCACCGGTGGCCGGGAACCCCCa                                                      <  1:196672/149‑1 (MQ=255)                                                                                             cGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CTTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTCGGCACCGGTGGCCGGGAACCCCCAg                                                     <  7:235949/149‑1 (MQ=255)                                                                                             cGCTGATCCACTCCGGCGCTTCGAAGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTCGGCACCGGTGGCCGGGAACCCCCAg                                                     <  8:143131/149‑1 (MQ=255)                                                                                              gCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTCGGCACCGGTGGCCGGGAACCCCCAgc                                                    >  4:258489/1‑149 (MQ=255)                                                                                                 gATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTCGGCACCGGTGGCCGGGAACCCCCAgcggc                                                 >  6:173853/1‑149 (MQ=255)                                                                                                     cACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGCTGCTCCAACACATCGGCCATACGCTCGGCACCGGTGGCCGGGAACCCCCAGCGgcgc                                               <  1:314918/148‑1 (MQ=255)                                                                                                     cACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTCGGCACCGGTGGCCGGGAACCCCCAGCGGCGCAg                                             >  6:346924/1‑149 (MQ=255)                                                                                                      aCTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTCGGCACCGGTGGCCGGGAACCCCCAGCGGCGCAgg                                            <  5:92238/149‑1 (MQ=255)                                                                                                      aCTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTCGGCACCGGTGGCCGGGAACCCCCAGCGGCGCAg                                             >  1:86480/1‑148 (MQ=255)                                                                                                          cGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTCGGCACCGGTGGCCg                                                                >  4:306399/1‑125 (MQ=255)                                                                                                          cGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTCGGCACCGGTGGCCg                                                                <  3:306399/125‑1 (MQ=255)                                                                                                           ggCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTCGGCACCGGTGGCCGGGAACCCCCAGCGGCGCAGGAACAg                                       <  2:27703/149‑1 (MQ=255)                                                                                                            gcgcTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTCGGCACCGGTGGCCGGGAACCCCCAGCGGCGCAGGAACAgg                                      <  5:300550/149‑1 (MQ=255)                                                                                                                ttCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTCGGCACCGGTGGCCGGGAACCCCCAGCGGCGCAGGAACAGGTCg                                   >  3:239297/1‑148 (MQ=255)                                                                                                                   gACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCTACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTCGGCACCGGAGGCCGGGAACCCCCAGCGGCGCAGGAACAGGTCGCAAt                               <  3:226216/149‑1 (MQ=255)                                                                                                                   gACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTCGGCACCGGTGGCCGGGAACCCCCAGCGGCGCAGGAACAGGTCGCAAt                               <  2:213904/149‑1 (MQ=255)                                                                                                                   gACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTCCGCACCGGTGGCCGGGAACCCCCAGCGGCGCAGGAACAGGTCGCat                                <  1:287566/148‑2 (MQ=255)                                                                                                                       cgGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTCGGCACCGGTGGCCGGGAACCCCCAGCGGCGCAGGAACAGGTCGCAATACAt                           <  2:86480/149‑1 (MQ=255)                                                                                                                        ggTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTCGGCACCGGTGGCCGGGAACCCCCAGCGGCGCAGGAACAGGTCGCAATACATg                          >  1:239520/1‑149 (MQ=255)                                                                                                                         gTAGACCAGCTGGATGGCAATCCGCTTAC‑CGTCCAGCAGGAACACCGAGCCGATCAGCAGCTCTCGCAGGC‑GCTCCAACACATCGGCCATACGCTCGGCACCGGTGGCCGGGAACCCCCAGCGGCGCAGGAACAGGTCGCAATACATGc                         <  2:101117/149‑1 (MQ=255)                                                                                                                           gttgctgCTGGACGGTAAGCCGATTGC‑CATCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTCGGCACCGGTGGCCGGGAACCCCCAGCGGCGCAGGAACAGGTCGCAATACATGCTg                       >  5:167847/7‑149 (MQ=38)                                                                                                                             acCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTCGGCACCGGTGGCCGGGAACCCCCAGCGGCGCa                                              >  2:229116/1‑124 (MQ=255)                                                                                                                             acCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTCGGCACCGGTGGCCGGGAACCCCCAGCGGCGCAGGAACAGGTCGCAATACATGCTGGc                     >  3:99406/1‑149 (MQ=255)                                                                                                                                tgCTGGACGGTAAGCCGATTGC‑CATCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTCGGCACCGGTGGCCGGGAACCCCCAGCGGCGCAGGAACAGGTc                                    <  7:279653/130‑1 (MQ=255)                                                                                                                                tgCTGGACGGTAAGCCGATTGC‑CATCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTCGGCACCGGTGGCCGGGAACCCCCAGCGGCGCAGGAACAGGTc                                    >  8:279653/2‑131 (MQ=255)                                                                                                                                tgCTGGACGGTAAGCCGATTGC‑CATCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTCGGCACCGGTGGCCGGGAACCCCCAGCGGCGCAGGAACAGGTCGCAATACATGCTGGCGAt                  >  7:71859/2‑149 (MQ=255)                                                                                                                                 gCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTCGGCACCGGTGGCCGGGAACCCCCAGCGGCGCAGGAACAGGTCGCAATACATGCTGGCGAtt                 >  8:192671/1‑149 (MQ=255)                                                                                                                                 gCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTCGGCACCGGTGGCCGGGAACCCCCAGCGGCGCAGGAACAGGTCGCAATACATGCTGGCGAtt                 <  5:173853/149‑1 (MQ=255)                                                                                                                                     gATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTCGGCACCGGTGGCCGGGAACCCCCAGCGGCGCAGGAACAGGTCGCAATACATGCTGGCGATTTCCt             >  8:338060/1‑149 (MQ=255)                                                                                                                                        ggCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTc                                                                              >  6:330751/1‑81 (MQ=255)                                                                                                                                        ggCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTc                                                                              <  5:330751/81‑1 (MQ=255)                                                                                                                                         gCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTCGGCACCGGTGGCCGGGAACCCCCAGCGGCGCAGGAACAGGTCGCAATACATGCTGGCGATTTCCTggg          <  4:239297/148‑1 (MQ=255)                                                                                                                                         gCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTCGGCACCGGTGGCCGGGAACCCCCAGCGGCGCAGGAACAGGTCGCAATACATGCTGGCGATTTCCTGGGc         >  2:179111/1‑149 (MQ=255)                                                                                                                                                gCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTCGGCACCGGTGGCCGGGAACCCCCCGCGGCGCAGGAACAGGTCGCAATACATGCTGGCGATTTCCTGGGCACTGAAg  >  2:258848/1‑149 (MQ=255)                                                                                                                                                  ttAC‑CGTCCAGCAGCAAGACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATATGGTCGGCACCGGTGGCCGGGAACCCCCAGCGGCGCAGGAACAGGTCg                                   <  6:38144/114‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                   TTCCCAGGCGGCCTGGGTATTGAGGAAGCTCAGCACGCTGCCTGGGCTGAAGGCCTTGGTTTCAGGGTCGACGCCGCCATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTCGGCACCGGTGGCCGGGAACCCCCAGCGGCGCAGGAACAGGTCGCAATACATGCTGGCGATTTCCTGGGCACTGAAG  >  NC_002947/5623836‑5624127

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑