Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,623,986	G→A	9.1%	D215D (GAC→GAT)	PP_4942 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,623,986	0	G	A	9.1%	213.0 / 8.7	78	D215D (GAC→GAT)	PP_4942	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (37/32);  new base A (3/4);  total (40/38)
Fisher's exact test for biased strand distribution p-value = 7.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.39e-01

CGGCCTGGGTATTGAGGAAGCTCAGCACGCTGCCTGGGCTGAAGGCCTTGGTTTCAGGGTCGACGCCGCCATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTCGGCACCGGTGGCCGGGAACCCCCAGCGGCGCAGGAACAGGTCGCAATACATGCTGGCGATTTCCTGGGCACTGAAGTCGCT  >  NC_002947/5623844‑5624132                                                                                                                                                |                                                                                                                                                    atgcCTGGGTATTGAGGAATCTCAGCTGTCTTCCTGGTCTGAAGGCAGTGGTTGCAGGGGAGACGCCGACATTGATGTACTCTCCTCTGATAAACTCCTGCGGTTAGAGGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CTTCaagc                                                                                                                                               <  4:384534/147‑1 (MQ=255)   gcCTGGGTATTGAGGAAGCTCAGCACGCTGCCTGGGCTGAAGGCCTTGGTTTCAGGGTCGACGCCGCCATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGACGGTAAGCCGATTGC‑CATCcagctg                                                                                                                                             <  6:160599/149‑3 (MQ=255)   gcCTGGGTATTGAGGAAGCTCAGCACGCTGCCTGGGCTGAAGGCCTTGGTTTCAGGGTCGACGCCGCCATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGACGGTAAGCCGATTGC‑CATCcagctg                                                                                                                                             <  6:288933/149‑3 (MQ=255)    ccTGGGTATTGAGGAAGCTCAGCACGCTGCCTGGGCTGAAGGCCTTGGTTTCAGGGTCGACGCCGCCATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTACATGTCcagcac                                                                                                                                             >  6:27478/1‑148 (MQ=255)    ccTGGGTATTGAGGAAGCTCAGCACGCTGCCTGGGCTGAAGGCCTTGGTTTCAGGGTCGACGCCGCCATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGACGGTAAGCCGATTGC‑CATCcagctgg                                                                                                                                            <  6:264573/149‑4 (MQ=255)         gTATTGAGGAAGCTCAGCACGCTGCCTGGGCTGAAGGCCTTGGTTTCAGGGTCGACGCCGCCATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAacac                                                                                                                                       >  4:174290/1‑149 (MQ=255)          tATTGAGGAAGCTCAGCACGCTGCCTGGGCTGAAGGCCTTGGTTTCAGGGTCGACGCCGCCATTGATGTACTCGACGCTGATACACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACAcc                                                                                                                                      >  8:235949/1‑149 (MQ=255)            ttGAGTAAGCTCCGCACGCTGCCTGGGCTGAAGGCCTTGGTTTCAGGGTCGACGCCGCCATGGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGa                                                                                                                                    <  8:334436/149‑1 (MQ=255)               aGGAAGCTCAGCACGCTGCCTGGGCTGAAGGCCTTGGTTTCAGGGTCGACGCCGCCATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGcc                                                                                                                                 >  4:87032/1‑149 (MQ=255)               aGGAAGCTCAGCACGCTGCCTGGGCTGAAGGCCTTGGTTTCAGGGTCGACGCCGCCATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGcc                                                                                                                                 >  4:92528/1‑149 (MQ=255)                   aGCTCAGCACGCTGCCTGGGCTGAAGGCCTTGGTTTCAGGGTCGACGCCGCCATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGAt                                                                                                                              >  2:287566/1‑148 (MQ=255)                    gCTCAGCACGCTGCCTGGGCTGAAGGCCTTGGTTTCAGGGTCGACGCCGCCATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATca                                                                                                                            <  5:367705/149‑1 (MQ=255)                      tCAGCACGCTGCCTGGGCTGAAGGCCTTGGTTTCAGGGTCGACGCCGCCATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATcag                                                                                                                           >  6:388252/1‑148 (MQ=255)                       cAGCACGCTGCCTGGGCTGAAGGCCTTGGTTTCAGGGTCGACGCCGCCATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATcag                                                                                                                           >  1:316239/1‑147 (MQ=255)                       cAGCACGCTGCCTGGGCTGAAGGCCTTGGTTTCAGGGTCGACGCCGCCATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATcag                                                                                                                           >  1:27703/1‑147 (MQ=255)                                 ccTGGGCTGAAGGCCTTGGTTTCAGGGTCGACGCCGCCATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGc                                                                                                                 <  3:174290/147‑1 (MQ=255)                                 ccTGGGCTGAAGGCCTTGGTTTCAGGGTCGACGCCGCCATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAg                                                                                                               <  3:185144/149‑1 (MQ=255)                                       cTGAAGGCCTTGGTTTCAGGGTCGACGCCGCCATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTc                                                                                                        <  2:316239/149‑1 (MQ=255)                                       cTGAAGGCCTTGGTTTCAGGGTCGACGCCGCCATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTc                                                                                                        >  8:186584/1‑149 (MQ=255)                                          aaGGCCTTGTTTTCTGTGTCGAGTCCGGCATTTATGTACTCGTCGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAG‑CGTCCAGCATCAACACCGAGCCGATca                                                                                                                            <  5:220745/127‑1 (MQ=255)                                          aaGGCCTTGGTTTCAGGGTCGACGCCGCCATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCaa                                                                                                     <  3:92528/149‑1 (MQ=255)                                          aaGGCCTTGGTTTCAGGGTCGACGCCGCCATTGATGTACTCGACGCTGATCCACTCAGGCGCTTCGACGCGGTACACCAGCTGGATGGCAAACGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATca                                                                                                                            >  6:220745/1‑127 (MQ=255)                                           aGGCCTTGGTTTCAGGGTCGACGCCGCCATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCaa                                                                                                     <  3:87032/148‑1 (MQ=255)                                           aGGCCTTGGTTTCAGGGTCGACGCCGCCATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAac                                                                                                    <  5:388252/149‑1 (MQ=255)                                               cTTGGTTTCAGGGTCGACGCCGCCATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAAcaca                                                                                                 >  8:150455/1‑148 (MQ=255)                                               cTTGGTTTCAGGGTCGACGCCGCCATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACAt                                                                                                >  6:92238/1‑149 (MQ=255)                                               cTTGGTTTCAGGGTCGACGCCGCCATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACAt                                                                                                <  7:150455/149‑1 (MQ=255)                                                ttGGTTTCAGGGTCGACGCCGCCATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATc                                                                                               >  1:213904/1‑149 (MQ=255)                                                  ggTTTCAGGGTCGACGCCGCCATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCgg                                                                                             >  6:300550/1‑149 (MQ=255)                                                    tttCAGGGTCGACGCCGCCATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGc                                                                                            >  2:314918/1‑148 (MQ=255)                                                     ttCAGGGTCGACGCCGCCATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAac                                                                                                    <  2:184714/139‑1 (MQ=255)                                                     ttCAGGGTCGACGCCGCCATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAac                                                                                                    >  1:184714/1‑139 (MQ=255)                                                          ggTCGACGCCGCCATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACCCATCGGCCATACGc                                                                                     >  2:315196/1‑149 (MQ=255)                                                          ggTCGACGCCGCCATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGc                                                                                     >  3:399996/1‑149 (MQ=255)                                                             cGACGCCGCCATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGAAGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTCg                                                                                  >  1:4128/1‑149 (MQ=255)                                                              gACGCCGCCATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTCgg                                                                                 <  4:399996/149‑1 (MQ=255)                                                              gACGCCGCCATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTCg                                                                                  <  7:186584/148‑1 (MQ=255)                                                                    gccATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTCGGCACCgg                                                                           >  2:390428/1‑149 (MQ=255)                                                                               aCTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTc                                                                                                        <  8:81328/109‑1 (MQ=255)                                                                               aCTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTc                                                                                                        >  7:81328/1‑109 (MQ=255)                                                                                cTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTCGGCACCGGTGGCCGGGAccc                                                               >  4:143911/1‑149 (MQ=255)                                                                                    aCGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTCGGCACCGGTGGCCGGGAACCCCCa                                                           <  1:196672/149‑1 (MQ=255)                                                                                     cGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CTTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTCGGCACCGGTGGCCGGGAACCCCCAg                                                          <  7:235949/149‑1 (MQ=255)                                                                                     cGCTGATCCACTCCGGCGCTTCGAAGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTCGGCACCGGTGGCCGGGAACCCCCAg                                                          <  8:143131/149‑1 (MQ=255)                                                                                      gCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTCGGCACCGGTGGCCGGGAACCCCCAgc                                                         >  4:258489/1‑149 (MQ=255)                                                                                         gATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTCGGCACCGGTGGCCGGGAACCCCCAgcggc                                                      >  6:173853/1‑149 (MQ=255)                                                                                             cACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGCTGCTCCAACACATCGGCCATACGCTCGGCACCGGTGGCCGGGAACCCCCAGCGgcgc                                                    <  1:314918/148‑1 (MQ=255)                                                                                             cACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTCGGCACCGGTGGCCGGGAACCCCCAGCGGCGCAg                                                  >  6:346924/1‑149 (MQ=255)                                                                                              aCTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTCGGCACCGGTGGCCGGGAACCCCCAGCGGCGCAgg                                                 <  5:92238/149‑1 (MQ=255)                                                                                              aCTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTCGGCACCGGTGGCCGGGAACCCCCAGCGGCGCAg                                                  >  1:86480/1‑148 (MQ=255)                                                                                                  cGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTCGGCACCGGTGGCCg                                                                     >  4:306399/1‑125 (MQ=255)                                                                                                  cGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTCGGCACCGGTGGCCg                                                                     <  3:306399/125‑1 (MQ=255)                                                                                                   ggCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTCGGCACCGGTGGCCGGGAACCCCCAGCGGCGCAGGAACAg                                            <  2:27703/149‑1 (MQ=255)                                                                                                    gcgcTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTCGGCACCGGTGGCCGGGAACCCCCAGCGGCGCAGGAACAgg                                           <  5:300550/149‑1 (MQ=255)                                                                                                        ttCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTCGGCACCGGTGGCCGGGAACCCCCAGCGGCGCAGGAACAGGTCg                                        >  3:239297/1‑148 (MQ=255)                                                                                                           gACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCTACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTCGGCACCGGAGGCCGGGAACCCCCAGCGGCGCAGGAACAGGTCGCAAt                                    <  3:226216/149‑1 (MQ=255)                                                                                                           gACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTCGGCACCGGTGGCCGGGAACCCCCAGCGGCGCAGGAACAGGTCGCAAt                                    <  2:213904/149‑1 (MQ=255)                                                                                                           gACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTCCGCACCGGTGGCCGGGAACCCCCAGCGGCGCAGGAACAGGTCGCat                                     <  1:287566/148‑2 (MQ=255)                                                                                                               cgGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTCGGCACCGGTGGCCGGGAACCCCCAGCGGCGCAGGAACAGGTCGCAATACAt                                <  2:86480/149‑1 (MQ=255)                                                                                                                ggTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTCGGCACCGGTGGCCGGGAACCCCCAGCGGCGCAGGAACAGGTCGCAATACATg                               >  1:239520/1‑149 (MQ=255)                                                                                                                 gTAGACCAGCTGGATGGCAATCCGCTTAC‑CGTCCAGCAGGAACACCGAGCCGATCAGCAGCTCTCGCAGGC‑GCTCCAACACATCGGCCATACGCTCGGCACCGGTGGCCGGGAACCCCCAGCGGCGCAGGAACAGGTCGCAATACATGc                              <  2:101117/149‑1 (MQ=255)                                                                                                                   gttgctgCTGGACGGTAAGCCGATTGC‑CATCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTCGGCACCGGTGGCCGGGAACCCCCAGCGGCGCAGGAACAGGTCGCAATACATGCTg                            >  5:167847/7‑149 (MQ=38)                                                                                                                     acCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTCGGCACCGGTGGCCGGGAACCCCCAGCGGCGCa                                                   >  2:229116/1‑124 (MQ=255)                                                                                                                     acCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTCGGCACCGGTGGCCGGGAACCCCCAGCGGCGCAGGAACAGGTCGCAATACATGCTGGc                          >  3:99406/1‑149 (MQ=255)                                                                                                                        tgCTGGACGGTAAGCCGATTGC‑CATCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTCGGCACCGGTGGCCGGGAACCCCCAGCGGCGCAGGAACAGGTc                                         <  7:279653/130‑1 (MQ=255)                                                                                                                        tgCTGGACGGTAAGCCGATTGC‑CATCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTCGGCACCGGTGGCCGGGAACCCCCAGCGGCGCAGGAACAGGTc                                         >  8:279653/2‑131 (MQ=255)                                                                                                                        tgCTGGACGGTAAGCCGATTGC‑CATCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTCGGCACCGGTGGCCGGGAACCCCCAGCGGCGCAGGAACAGGTCGCAATACATGCTGGCGAt                       >  7:71859/2‑149 (MQ=255)                                                                                                                         gCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTCGGCACCGGTGGCCGGGAACCCCCAGCGGCGCAGGAACAGGTCGCAATACATGCTGGCGAtt                      <  5:173853/149‑1 (MQ=255)                                                                                                                         gCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTCGGCACCGGTGGCCGGGAACCCCCAGCGGCGCAGGAACAGGTCGCAATACATGCTGGCGAtt                      >  8:192671/1‑149 (MQ=255)                                                                                                                             gATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTCGGCACCGGTGGCCGGGAACCCCCAGCGGCGCAGGAACAGGTCGCAATACATGCTGGCGATTTCCt                  >  8:338060/1‑149 (MQ=255)                                                                                                                                ggCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTc                                                                                   <  5:330751/81‑1 (MQ=255)                                                                                                                                ggCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTc                                                                                   >  6:330751/1‑81 (MQ=255)                                                                                                                                 gCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTCGGCACCGGTGGCCGGGAACCCCCAGCGGCGCAGGAACAGGTCGCAATACATGCTGGCGATTTCCTggg               <  4:239297/148‑1 (MQ=255)                                                                                                                                 gCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTCGGCACCGGTGGCCGGGAACCCCCAGCGGCGCAGGAACAGGTCGCAATACATGCTGGCGATTTCCTGGGc              >  2:179111/1‑149 (MQ=255)                                                                                                                                        gCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTCGGCACCGGTGGCCGGGAACCCCCCGCGGCGCAGGAACAGGTCGCAATACATGCTGGCGATTTCCTGGGCACTGAAg       >  2:258848/1‑149 (MQ=255)                                                                                                                                          ttAC‑CGTCCAGCAGCAAGACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATATGGTCGGCACCGGTGGCCGGGAACCCCCAGCGGCGCAGGAACAGGTCg                                        <  6:38144/114‑1 (MQ=255)                                                                                                                                             c‑cGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTCGGCACCGGTGGCCGGGAACCCCCAGCGGCGCAGGAACAGGTCGCAATACATGCTGGCGATTTCCTGGGCACTGAAGTCGc   <  4:99406/148‑1 (MQ=255)                                                                                                                                             c‑cGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTCGGCACCGGTGGCCGGGAACCCCCAGCGGCGCAGGAACAGGTCGCAATACATGCTGGCGATTTCCTGGGCACTGAAGTCGCt  <  2:239520/149‑1 (MQ=255)                                                                                                                                                |                                                                                                                                                    CGGCCTGGGTATTGAGGAAGCTCAGCACGCTGCCTGGGCTGAAGGCCTTGGTTTCAGGGTCGACGCCGCCATTGATGTACTCGACGCTGATCCACTCCGGCGCTTCGACGCGGTACACCAGCTGGATGGCAATCGGCTTAC‑CGTCCAGCAGCAACACCGAGCCGATCAGCAGCTCACGCAGGC‑GCTCCAACACATCGGCCATACGCTCGGCACCGGTGGCCGGGAACCCCCAGCGGCGCAGGAACAGGTCGCAATACATGCTGGCGATTTCCTGGGCACTGAAGTCGCT  >  NC_002947/5623844‑5624132

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑