Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,640,100	G→C	6.8%	G398R (GGC→CGC) ‡	PP_4950 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,640,100	0	G	C	6.8%	219.4 / 5.0	69	G398R (GGC→CGC) ‡	PP_4950	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (32/32);  new base C (3/2);  total (35/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGTACCTGAACTACTCAGACCTGCCAGCGGCGCGCATGACCGGGCTGACCCGCTTCGCCACTTTCTGGGTAGAAGACGGGCAGATCCAGGGGCCGGTGAGTACCATGCGCTTCGATGACAGCTTGTACAGCTTGCTGGGCAGCCAGCTGGAGGACCTGACCCAGGAGCGCGAGATGATCCTGTCGACCAGTACTTATGGACAGCGCAGTACCGGGTCGAGTCATTTGCCCGGGGCACTGGTCAAAGGCCTGACCCTGACATTGTGACTGGACTTGCCGGCCCTTT  >  NC_002947/5639963‑5640247                                                                                                                                          |                                                                                                                                                    ggTACCTGAACTACTCAGACCTGCCAGCGGCGCGCATGACCGGGCTGACCCGCTTCGCCACTTTCTGGGTAGAAGACGGGCAGATCCAGGGGCCGGTGAGTACCATGCGCTTCGATGACAGCTTGTACAGCTTGCTGGGCAGCCAGCTg                                                                                                                                          >  7:40436/1‑149 (MQ=255)  gTACCTGAACTACTCAGACCTGCCAGCGGCGCGCATGACCGGGCTGACCCGCTTCGCCACTTTCTGGGTAGAAGACGGGCAGATCCAGGGGCCGGTGAGTACCATGCGCTTCGATGACAGCTTGTACAGCTTGCTGGGCAGCCAGCTg                                                                                                                                          <  3:217700/148‑1 (MQ=255)     ccTGAACTACTCAGACCTGCCAGCGGCGCGCATGACCGGGCTGACCCGCTTCGCCACTTTCTGGGTAGAAGACGGGCAGATCCAGGGGCCGGTGAGTACCATGCGCTTCGATGACAGCTTGTACAGCTTGCTGGGCAGCCAGCTggag                                                                                                                                       <  3:308622/148‑1 (MQ=255)       tGAACTACTCAGACCTGCCAGCGGCGCGCATGACCGGGCTGACCCGCTTCGCCAATTTCTGGGTAGAAGACGGGCAGATCCAGGGGCCGGTGAGTACCATGCGCTTCGATGACAGCTTGTACAGCTTGCTGGGCAGCCAGCTGGAGGAc                                                                                                                                    >  1:310815/1‑149 (MQ=255)         aaCTACTCAGACCTGCCAGCGGCGCGCATGACCGGGCTGACCCGCTTCGCCACTTTCTGGGTAGAAGACGGGCAGATCCAGGGGCCGGTGAGTACCATGCGCTTCGATGACAGCTTGTACAGCTTGCTGGGCAGCCAGCTGGAGGAc                                                                                                                                    <  2:319246/147‑1 (MQ=255)         aaCTACTCAGACCTGCCAGCGGCGCGCATGACCGGGCTGACCCGCTTCGCCACTTTCTGGGTAGAAGACGGGCAGATCCAGGGGCCGGTGAGTACCATGCGCTTCGATGACAGCTTGTACAGCTTGCTGGGCAGCCAGCTGGAGGACCt                                                                                                                                  >  4:252894/1‑149 (MQ=255)           ctactCAGACCTGCCAGCGGCGCGCATGACCGGGCTGACCCGCTTCGCCACTTTCTGGGTAGAAGACGGGCAGATCCAGGGGCCGGTGAGTACCATGCGCTTCGATGACAGCTTGTACAGCTTGCTGGGCAGCCAGCTGGAGGACCTGa                                                                                                                                <  4:196916/149‑1 (MQ=255)              ctCAGACCTGCCAGCGGCGCGCATGACCGGGCTGACCCGCTTCGCCACTTTCTGGGTAGAAGACGGGCAGATCCAGGGGCCGGTGAGTACCATGCGCTTCGATGACAGCTTGTACAGCTTGCTGGGCAGCCAGCTGGAGGACCTGAccc                                                                                                                             <  6:266204/149‑1 (MQ=255)              ctCAGACCTGCCAGCGGCGCGCATGACCGGGCTGACCCGCTTCGCCACTTTCTGGGTAGAAGACGGGCAGATCCAGGGGCCGGTGAGTACCATGCGCTTCGATGACAGCTTGTACAGCTTGCTGGGCAGCCAGCTGGAGGACCTGAcc                                                                                                                              >  6:180066/1‑148 (MQ=255)              ctCAGACCTGCCAGCGGCGCGCATGACCGGGCTGACCCGCTTCGCCACTTTCTGGGTAGAAGACGGGCAGATCCAGGGGCCGGTGAGTACCATGCGCTTCGATGACAGCTTGTACAGCTTGCTGGGCAGCCAGCTGGAGGACCTGAcc                                                                                                                              >  6:324537/1‑148 (MQ=255)               tCAGACCTGCCAGCGGCGCGCATGACCGGGCTGACCCGCTTCGCCACTTTCTGGGTAGAAGACGGGCAGATCCAGGGGCCGGTGAGTACCATGCGCTTCGATGACAGCTTGTACAGCTTGCTgg                                                                                                                                                     >  2:140451/1‑124 (MQ=255)               tCAGACCTGCCAGCGGCGCGCATGACCGGGCTGACCCGCTTCGCCACTTTCTGGGTAGAAGACGGGCAGATCCAGGGGCCGGTGAGTACCATGCGCTTCGATGACAGCTTGTACAGCTTGCTgg                                                                                                                                                     <  1:140451/124‑1 (MQ=255)                 aGACCTGCCAGCGGCGCGCATGACCGGGCTGACCCGCTTCGCCACTTTCTGGGTAGAAGACGGGCAGATCCAGGGGCCGGTGAGTACCATGCGCTTCGATGACAGCTTGTACAGCTTGCTGGGCAGCCAGCTGGAGGACCTGACCCAg                                                                                                                           >  1:233311/1‑148 (MQ=255)                    ccTGCCAGCGGCGCGCATGACCGGGCTGACCCGCTTCGCCACTTTCTGGGTAGAAGACGGGCAGATCCAGGGGCCGGTGAGTACCATGCGCTTCGATGACAGCTTGTACAGCTTGCTGGGCAGCCAGCTGGAGGACCTGACCCAGGAgc                                                                                                                       >  8:146322/1‑149 (MQ=255)                        ccAGCGGCGCGCATGACCGGGCTGACCCGCTTCGCCACTTTCTGGGTAGAAGACGGGCAGATCCAGGGGCCGGTGAGTACCATGCGCTTCGATGACAGCTTGTACAGCTTGCTGGGCAGCCAGCTGGAGGACCTGACCCAGGAgcgc                                                                                                                     >  3:334479/1‑147 (MQ=255)                               cgcgcATGACCGGGCTGACCCGCTTCGCCACTTTCTGGGTAGAAGACGGGCAGATCCAGGGGCCGGTGAGTACCATGCGCTTCGATGACAGCTTGTACAGCTTGCTGGGCAGCCAGCTGGAGGACCTGACCCAGGAGCGCGAGATGATc                                                                                                            <  5:147307/149‑1 (MQ=255)                                   cATGACCGGGCTGACCCGCTTCGCCACTTTCTGGGTAGAAGACGGGCAGATCCAGGGGCCGGTGAGTACCATGCGCTTCGATGACAGCTTGTACAGCTTGCTGGGCAGCCAGCTGGAGGACCTGACCCAGGAGCGCGAGATGATCCTGt                                                                                                        <  2:233311/149‑1 (MQ=255)                                      gACCGGGCTGACCCGCTTCGCCACTTTCTGGGTAGAAGACGGGCAGATCCAGGGGCCGGTGAGTACCATGCGCTTCGATGACAGCTTGTACAGCTTGCTGGGCAGCCAGCTGGAGGACCTGACCCAGGAGCGCGAGATGATc                                                                                                            >  8:98418/1‑142 (MQ=255)                                      gACCGGGCTGACCCGCTTCGCCACTTTCTGGGTAGAAGACGGGCAGATCCAGGGGCCGGTGAGTACCATGCGCTTCGATGACAGCTTGTACAGCTTGCTGGGCAGCCAGCTGGAGGACCTGACCCAGGAGCGCGAGATGATc                                                                                                            <  7:98418/142‑1 (MQ=255)                                       aCCGGGCTGACCCGCTTCGCCACTTTCTGGGTAGAAGACGGGCAGATCCAGGGGCCGGTGAGTACCATGCGCTTCGATGACAGCTTGTACAGCTTGCTGGGCAGCCAGCTGGAGGACCTGACCCAGGAGCGCgaga                                                                                                                 >  2:82744/1‑136 (MQ=255)                                       aCCGGGCTGACCCGCTTCGCCACTTTCTGGGTAGAAGACGGGCAGATCCAGGGGCCGGTGAGTACCATGCGCTTCGATGACAGCTTGTACAGCTTGCTGGGCAGCCAGCTGGAGGACCTGACCCAGGAGCGCGAGATGATCCTGTCGAc                                                                                                    <  3:252894/149‑1 (MQ=255)                                       aCCGGGCTGACCCGCTTCGCCACTTTCTGGGTAGAAGACGGGCAGATCCAGGGGCCGGTGAGTACCATGCGCTTCGATGACAGCTTGTACAGCTTGCTGGGCAGCCAGCTGGAGGACCTGACCCAGGAGCGCGAGATGATCCTGTCGAc                                                                                                    <  7:146322/149‑1 (MQ=255)                                       aCCGGGCTGACCCGCTTCGCCACTTTCTGGGTAGAAGACGGGCAGATCCAGGGGCCGGTGAGTACCATGCGCTTCGATGACAGCTTGTACACCTTGCTGGGCAGCCAGCTGGAGGACCTGACCCAGGAGCGCgaga                                                                                                                 <  1:82744/136‑1 (MQ=255)                                           ggCTGACCCGCTTCGCCACTTTCTGGGTAGAAGACGGGCAGATCCAGGGGCCGGTGAGTACCATGCGCTTCGATGACAGCTTGTACAGCTTGCTGGGCAGCCAGCTGGAGGACCTGACCCAGGAGCGCGAGATGATCCTGTCGACCAg                                                                                                 >  2:1204/1‑148 (MQ=255)                                                  ccGCTTCGCCACTTTCTGGGTAGAAGACGGGCAGATCCAGGGGCCGGTGAGTACCTTGCGCTTCGATGACAGCTTGTACAGCTTGCTGGGCAGCCAGCTGGAGGACCTGACCCAGGCGCTCGAGATGATCCTGTCGACCAGTACTTATg                                                                                         >  4:267048/1‑149 (MQ=255)                                                    gCTTCGCCACTTTCTGGGTAGAAGACGGGCAGATCCAGGGGCCGGTGAGTACCATGCGCTTCGATGACAGCTTGTACAGCTTGCTGGGCAGCCAGCTGGAGGAc                                                                                                                                    >  2:367032/1‑104 (MQ=255)                                                    gCTTCGCCACTTTCTGGGTAGAAGACGGGCAGATCCAGGGGCCGGTGAGTACCATGCGCTTCGATGACAGCTTGTACAGCTTGCTGGGCAGCCAGCTGGAGGAc                                                                                                                                    <  1:367032/104‑1 (MQ=255)                                                      ttCGCCACTTTCTGGGTAGAAGACGGGAAGATCCAGGGGCCGGTGAGTACCATGCGCTTCGATGACAGCTTGTACAGCTTGCTGGGCAGCCAGCTGGAGGACCTGACCCAGGAGCGCGAGATGATCCTGTCGACCAGTACTTATGGACa                                                                                     <  3:29736/149‑1 (MQ=255)                                                         tccACTTTCAGGGTAGAAGACGGGCAGATCCAGGGGCCGGTGAGTACCATGAGCTTCGATGACAGCTAGTACAGCTTGATTCGCAGCGAGCTGGAGGACCTGACCCAGGAGCGCGAGAAGATCCTGTCGAGCAGTACATATGGACcgcg                                                                                  >  8:154311/2‑149 (MQ=255)                                                         gCCACTTTCTGGGTAGAAGACGGGCAGATCCAGGGGCCGGTGAGTACCATGCGCTTCGATGACAGCTTGTACAGCTTGCTGGGCAGCCAGCTGGAGGACCTGACCCAGGAGCGCGAGATGATc                                                                                                            >  8:241710/1‑123 (MQ=255)                                                         gCCACTTTCTGGGTAGAAGACGGGCAGATCCAGGGGCCGGTGAGTACCATGCGCTTCGATGACAGCTTGTACAGCTTGCTGGGCAGCCAGCTGGAGGACCTGACCCAGGAGCGCGAGATGATc                                                                                                            <  7:241710/123‑1 (MQ=255)                                                         gCCACTTTCTGGGTAGAAGACGGGCAGATCCAGGGGCCGGTGAGTACCATGCGCTTCGATGACAGCTTGTACAGCTTGCTGGGCAGCCAGCTGGAGGACCTGACCCAGGAGCGCGAGATGATCCTGTCGACCAGTACTTATGGACAgca                                                                                  >  8:187652/1‑148 (MQ=255)                                                          ccACTTTCTGGGTAGAAGACGGGCAGATCCAGGGGCCGGTGAGTACCATGCGCTTCGATGACAGCTTGTACAGCTTGCTGGGCAGCCAGCTGGAGGACCTGACCCAGGAGCGCGAGATGATCCTGTCGACCAGTACTTATGGACAgcg                                                                                  >  3:357042/1‑148 (MQ=255)                                                                tCTGGGTAGAAGACGGGCAGATCCAGGGGCCGGTGAGTACCATGCGCTTCGATGACAGCTTGTACAGCTTGCTGGGCAGCCAGCTGGAGGACCTGACCCAGGAGCGCGAGATGATCCTGTCGACCAGTACTTATGGACAGCGCAGTAcc                                                                           >  8:42451/1‑149 (MQ=255)                                                                 cTGGGTAGAAGACGGGCAGATCCAGGGGCCGGTGAGTACCATGCGCTTCGATGACAGCTTGTACAGCTTGCTGGGCAGCCAGCTGGAGGACCTGACCCAGGAGCGCGAGATGATCCTGTCGACCAGTACTTATGGACAGCGCAGTAcc                                                                           <  2:310815/148‑1 (MQ=255)                                                                    ggTAGAAGACGGGCAGATCCAGGGGCCGGTGAGTACCATGCGCTTCGATGACAGCTTGTACAGCTTGCTGGGCAGCCAGCTGGAGGACCTGACCCAGGAGCGCGAGATGATCCTGTCGACCAGTACTTATGGACAGCGCAGTACCGGGt                                                                       >  7:182790/1‑149 (MQ=255)                                                                        gaagaCGGGCAGATCCAGGGGCCGGTGAGTACCATGCGCTTCGATGACAGCTTGTACAGCTTGCTGGGCAGCCAGCTGGAGGACCTGACCCAGGAGCGCGAGATGATCCTGTCGACCAGTACTTATGGACAGCGCAGTACCGGGTCGa                                                                    <  1:1204/148‑1 (MQ=255)                                                                              gggCAGATCCAGGGGCCGGTGAGTACCATGCGCTTCGATGACAGCTTGTACAGCTTGCTGGGCAGCCAGCTGGAGGACCTGACCCAGGAGCGCGAGATGATCCTGTCGACCAGTACTTATGGACAGCGCAGTACCGGGTCGAGTCAttt                                                             >  1:63493/1‑149 (MQ=255)                                                                                      ccAGGGGCCGGTGAGTACCATGCGCTTCGATGACAGCTTGTACAGCTTGCTGGGCAGCCAGCTGGAGGACCTGACCCAGGAGCGCGAGATGATCCTGTCGACCAGTACTTATGGACAGCGCAGTACCGGGTCGAGTCATTTGCCCgggg                                                     >  3:15546/1‑149 (MQ=255)                                                                                      ccAGGGGCCGGTGAGTACCATGCGCTTCGATGACAGCTTGTACAGCTTGCTGGGCAGCCAGCTGGAGGACCTGACCCAGGAGCGCGAGATGATCCTGTCGACCAGTACTTATGGACAGCGCAGTACCGGGTCGAGTCATTTGCCCggg                                                      <  2:63493/148‑1 (MQ=255)                                                                                       cAGGGGCCGGTGAGTACCATGCGCTTCGATGACAGCTTGTACAGCTTGCTGGGCAGCCAGCTGGAGGACCTGACCCAGGAGCGCGAGATGATCCTGTCGACCAGTACTTATGGACAGCGCAGTACCGGGTCGAGTCATTTGCCCgggg                                                     <  5:180066/148‑1 (MQ=255)                                                                                        aGGGGCCGGTGAGTACCATGCGCTTCGATGACAGCTTGTACAGCTTGCTGGGCAGCCAGCTGGAGGACCTGACCCAGGAGCGCGAGATGATCCTGTCGACCAGTACTTATGGACAGCGCAGTACCGGGTCGAGTCATTTGCCCGGGGCa                                                   >  5:76425/1‑149 (MQ=255)                                                                                                   aGTACCATGCGCTTCGATGACAGCTTGTACAGCTTGCTGGGCAGCCAGCTGGAGGACCTGACCCAGGAGCGCGAGATGATCCTGTCGACCAGTACTTATGGACAGCGCAGTACCGGGTCGAGTCATTTGCCCGGGGCACTGGTCAAAgg                                        >  2:328125/1‑149 (MQ=255)                                                                                                   aGTACCATGCGCTTCGATGACAGCTTGTACAGCTTGCTGGGCAGCCAGCTGGAGGACCTGACCCAGGAGCGCGAGATGATCCTGTCGACCAGTACTTATGGACAGCGCAGTACCGGGTCGAGTCATTTGCCCGGGGCACTGGTCAAAg                                         <  4:357042/148‑1 (MQ=255)                                                                                                   aGTACCATGCGCTTCGATGACAGCTTGTACAGCTTGCTGGGCAGCCAGCTGGAGGACCTGACCCAGGAGCGCGAGATGATCCTGTCGACCAGTACTTATGGACAGCGCAGTACCGGGTCGAGTCATTTGCCCGGGGCACTGGTCAAAg                                         <  8:40436/148‑1 (MQ=255)                                                                                                    gTACCATGCGCTTCGATGACAGCTTGTACAGCTTGCTGGGCAGCCAGCTGGAGGACCTGACCCAGGAGCGCGAGATGATCCTGTCGACCAGTACTTATGGACAGCGCAGTACCGGGTCGAGTCATTTGCCCGGGGCACTGGTCAAAGGc                                       <  4:334479/149‑1 (MQ=255)                                                                                                       ccATGCGCTTCGATGACAGCTTGTACAGCTTGCTGGGCAGCCAGCTGGAGGACCTGACCCAGGAGCGCGAGATGATCCTGTCGACCAGTACTTATGGACAGCGCAGTACCGGGTCGAGTCATTTGCCCGGGGCACTGGTCAAAGGcct                                     >  2:57963/1‑148 (MQ=255)                                                                                                          tGCGCTTCGATGACAGCTTGTACAGCTTGCTGGGCAGCCAGCTGGAGGACCTGACCCAGGAGCGCGAGATGATCCTGTCGACCAGTACTTATGGACAGCGCAGTACCGGGTCGAGTCATTTGCCCGGGGCACTGGTCAAAGGcctgacc                                 <  7:42451/149‑1 (MQ=255)                                                                                                                tCGATGACAGCTTGTACAGCTTGCTGGGCAGCCAGCTGGAGGACCTGACCCAGGAGCGCGAGATGATCCTGTCGACCAGTACTTATGGACAGCGCAGTACCgg                                                                         >  1:153001/1‑103 (MQ=255)                                                                                                                tCGATGACAGCTTGTACAGCTTGCTGGGCAGCCAGCTGGAGGACCTGACCCAGGAGCGCGAGATGATCCTGTCGACCAGTACTTATGGACAGCGCAGTACCgg                                                                         <  2:153001/103‑1 (MQ=255)                                                                                                                   aTGACAGCTTGTACAGCTTGCTGGGCAGCCAGCTGGAGGACCTGACCCAGGAGCGCGAGATGATCCTGTCGACCAGTACTTATGGACAGCGCAGTACCGGGTCGAGTCATTTGCCCGGGGCACTGGTCAAAGGCCTGACCCTGACATtg                        <  1:328125/149‑1 (MQ=255)                                                                                                                    tGACAGCTTGTACAGCTTGCTGGGCAGCCAGCTGGAGGACCTGACCCAGGAGCGCGAGATGATCCTGTCGACCAGTACTTATGGACAGCGCAGTACCGGGTCGAGTCATTTGCCCGGGGCACTGGTCAAAGGCCTGACCCTGACATtgt                       <  6:76425/149‑1 (MQ=255)                                                                                                                      aCAGCTTGTACAGCTTGCTGGGCAGCCAGCTGGAGGACCTGACCCAGGAGCGCGAGATGATCCTGTCGACCAGTACTTATGGACAGCGCAGTACCGGGTCGAGTCTTTTGCCCGGGGCACTGGTCAAAGGCCTGACCCTGACATTGTGa                     >  7:72911/1‑149 (MQ=255)                                                                                                                              tccAGCTGGCTGCCCAGCAAGCTGGAGGACCTGACCCAGGAGCGCGAGATGATCCTGTCGACCAGTACTTATGGACAGCGCAGTACCGGGTCGAGTCATTTGCCCGGGGCACTGGTCAAAGGcctgaccct                               <  8:317353/129‑1 (MQ=255)                                                                                                                              tccAGCTGGCTGCCCAGCAAGCTGGAGGACCTGACCCAGGAGCGCGAGATGATCCTGTCGACCAGTACTTATGGACAGCGCAGTACCGGGTCGAGTCATTTGCCCGGGGCACTGGTCAAAGGcctgaccct                               >  7:137182/3‑131 (MQ=255)                                                                                                                              tccAGCTGGCTGCCCAGCAAGCTGGAGGACCTGACCCAGGAGCGCGAGATGATCCTGTCGACCAGTACTTATGGACAGCGCAGTACCGGGTCGAGTCATTTGCCCGGGGCACTGGTCAAAGGcctgaccct                               >  7:317353/3‑131 (MQ=255)                                                                                                                              tccAGCTGGCTGCCCAGCAAGCTGGAGGACCTGACCCAGGAGCGCGAGATGATCCTGTCGACCAGTACTTATGGACAGCGCAGTACCGGGTCGAGTCATTTGCCCGGGGCACTGGTCAAAGGcctgaccct                               <  8:137182/129‑1 (MQ=255)                                                                                                                                cAGCTTGCTGGGCAGCCAGCTGGAGGACCTGACCCAGGAGCGCGAGATGATCCTGTCGACCAGTACTTATGGACAGCGCAGTACCGGGTCGAGTCATTTGCCCGGGGCACTGGTCAAAGGCCTGACCCTGACATTGTGACTGGACTTGc           >  7:16427/1‑149 (MQ=255)                                                                                                                                 aGCTTGCTGGGCAGCCAGCTGGAGGACCTGACCCAGGAGCGCGAGATGATCCt                                                                                                          <  6:79518/53‑1 (MQ=255)                                                                                                                                 aGCTTGCTGGGCAGCCAGCTGGAGGACCTGACCCAGGAGCGCGAGATGATCCt                                                                                                          >  5:79518/1‑53 (MQ=255)                                                                                                                                 aGCTTGCTGGGCAGCCAGCTGGAGGACCTGACCCAGGAGCGCGAGATGATCCTGTCGACCAGTACTTATGGACAGCGCAGTACCGGGTCGAGTCATTTGCCCGGGGCACTGGTCAAAGGCCTGACCCTGACATTGTGACTGGACTTGcc          >  7:362547/1‑149 (MQ=255)                                                                                                                                   cTTGCTGGGCAGCCAGCTGGAGGACCTGACCCAGGAGCGCGAGATGATCCTGTCGACCAGTACTTATGGACAGCGCAGTACCGGGTCGAGTCATTTGCCCGGGGCACTGGTCAAAGGCCTGACCCTGACATTGTGACTGGACTTGCCgg        <  8:72911/149‑1 (MQ=255)                                                                                                                                   cTTGCTGGGCAGCCAGCTGGAGGACCTGACCCAGGAGCGCGAGATGATCCTGTCGACCAGTACTTATGGACAGCGCAGTACCGGGTCGAGTCATTTGCCCGGGGCACTGGTCAAAGGCCTGACCCTGAAATTGTGACTGGACTTGCCg         <  8:362547/148‑1 (MQ=255)                                                                                                                                    ttGCTGGGCAGCCAGCTGGAGGACCTGACCCAGGAGCGCGAGATGATCCTGTCGACCAGTACTTATGGACAGCGCAGTACCGGGTCGAGTCATTTGCCCGGGGCACTGGTCAAAg                                         >  5:97915/1‑115 (MQ=255)                                                                                                                                    ttGCTGGGCAGCCAGCTGGAGGACCTGACCCAGGAGCGCGAGATGATCCTGTCGACCAGTACTTATGGACAGCGCAGTACCGGGTCGAGTCATTTGCCCGGGGCACTGGTCAAAg                                         <  6:97915/115‑1 (MQ=255)                                                                                                                                    ttGCTGGGCAGCCAGCTGGAGGACCTGACCCAGGAGCGCGAGATGATCCTGTCGACCAGTACTTATGGACAGCGCAGTACCGGGTCGAGTCATTTGCCCGGGGCACTGGTCAAAGGCCTGACCCTGACATTGTGACTGGACTTGCCGGc       >  5:218853/1‑149 (MQ=255)                                                                                                                                      gCTGGGCAGCCAGCTGGAGGACCTGACCCAGGAGCGCGAGATGATCCTGTCGACCAGTACTTATGGACAGCGCAGTACCGGGTCGAGTCATTTGCCCGGGGCACTGGTCAAAGGCCTGACCCTGACATTGTGACTGGACTTGCCGGcc      <  4:15546/148‑1 (MQ=255)                                                                                                                                       cTGGGCAGCCAGCTGGAGGACCTGACCCAGGAGCGCGAGATGATCCTGTCGACCAGTACTTATGGACAGCGCAGTACCGGGTCGAGTCATTTGCCCGGGGCACTGGTCaaa                                          <  7:376004/111‑1 (MQ=255)                                                                                                                                       cTGGGCAGCCAGCTGGAGGACCTGACCCAGGAGCGCGAGATGATCCTGTCGACCAGTACTTATGGACAGCGCAGTACCGGGTCGAGTCATTTGCCCGGGGCACTGGTCaaa                                          >  8:376004/1‑111 (MQ=255)                                                                                                                                        tGGGCAGCCAGCTGGAGGACCTGACCCAGGAGCGCGAGATGATCCTGTCGACCAGTACTTATGGACAGCGCAGTACCGGGTCGAGTCATTTGCCCGGGGCACTGGTCAAAGGcctgac                                  <  8:140557/118‑1 (MQ=255)                                                                                                                                        tGGGCAGCCAGCTGGAGGACCTGACCCAGGAGCGCGAGATGATCCTGTCGACCAGTACTTATGGACAGCGCAGTACCGGGTCGAGTCATTTGCCCGGGGCACTGGTCAAAGGcctgac                                  >  7:140557/1‑118 (MQ=255)                                                                                                                                         gggCAGCCAGCTGGAGGACCTGACCCAGGAGCGCGAGATGATCCTGTCGACCAGTACTTATGGACAGCGCAGTACCGGGTCGAGTCATTTGCCCGGGGCACTGGTCAAAGGCCTGACCCTGACATTGTGACTGGACTTGCCGGCCCttt  >  5:329722/1‑149 (MQ=255)                                                                                                                                          ggCAGCCAGCTGGAGGACCTGACCCAGGAGCGCGAGATGATCCTGTCGACCAGTACTTATGGACAGCGCAGTACCGGGTCGAGTCATTTGCCCGGGGCACTGGTCAAAGGCCTGACCCTGACATtg                        <  6:325318/126‑1 (MQ=255)                                                                                                                                          ggCAGCCAGCTGGAGGACCTGACCCAGGAGCGCGAGATGATCCTGTCGACCAGTACTTATGGACAGCGCAGTACCGGGTCGAGTCATTTGCCCGGGGCACTGGTCAAAGGCCTGACCCTGACATtg                        >  5:325318/1‑126 (MQ=255)                                                                                                                                          |                                                                                                                                                    GGTACCTGAACTACTCAGACCTGCCAGCGGCGCGCATGACCGGGCTGACCCGCTTCGCCACTTTCTGGGTAGAAGACGGGCAGATCCAGGGGCCGGTGAGTACCATGCGCTTCGATGACAGCTTGTACAGCTTGCTGGGCAGCCAGCTGGAGGACCTGACCCAGGAGCGCGAGATGATCCTGTCGACCAGTACTTATGGACAGCGCAGTACCGGGTCGAGTCATTTGCCCGGGGCACTGGTCAAAGGCCTGACCCTGACATTGTGACTGGACTTGCCGGCCCTTT  >  NC_002947/5639963‑5640247

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑