| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 461,030 | C→G | 5.1% | V479L (GTG→CTG) | pqqF ← | coenzyme PQQ synthesis protein F |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 461,030 | 0 | C | G | 5.1% | 257.9 / 3.2 | 79 | V479L (GTG→CTG) | pqqF | coenzyme PQQ synthesis protein F |
| Reads supporting (aligned to +/- strand): ref base C (39/36); new base G (2/2); total (41/38) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.67e-01 | |||||||||||
GGCAGGCTACCCCGTACCGCGTCTGGCAGTTGCTTGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCC‑‑CCGG‑CACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACGTTCCTGCAGTGGCGCCAGGGCCTGTTCCAGCACACGCTGTAACGGCTGGCGCATAGGTGA > NC_002947/460885‑461174 | ggCAGGCTACCCCGTACCGCGTCTGGCAGTTGCTTGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCActgc > 6:54171/1‑149 (MQ=255) aGGCTACCCCGTACCGCGTCTGGCAGTTGCTTGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCActgctgc > 1:111921/1‑149 (MQ=255) aGGCTACCCCGTACCGCGTCTGGCAGTTGCTTGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCActgctgc < 6:6858/149‑1 (MQ=255) aCCCCGTACCGCGTCTGGCAGTTGCTTGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCTgg > 8:31289/1‑147 (MQ=255) ccccGTACCGCGTCTGGCAGTTGCTTGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCTGGc < 6:281613/147‑1 (MQ=255) ccccGTACCGCGTCTGGCAGTTGCTTGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAg < 3:63192/149‑1 (MQ=255) ccccGTACCGCGTCTGGCAGTTGCTTGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAg < 2:111921/149‑1 (MQ=255) ccccGTACCGCGTCTGGCAGTTGCTTGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAACCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCTGGc < 1:15267/147‑1 (MQ=255) cccGTACCGCGTCTGGCAGTTGCTTGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAgg > 4:359054/1‑149 (MQ=255) cccGTACCGCGTCTGGCAGTTGCTTGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAgg > 3:39102/1‑149 (MQ=255) cccGTACCGCGTCTGGCAGTTGCTTGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCAGCAGCAGTGCTGCGGGCAgt < 4:307971/149‑2 (MQ=255) cGTACCGCGTCTGGCAGTTGCTTGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAGGcc > 2:178619/1‑149 (MQ=255) aCCGCGTCTGGCAGTTGCTTGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAGGCCGgc > 3:287013/1‑149 (MQ=255) cgcgTCTGGCAGTTGCTTGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAGGCCGgcg > 8:131797/1‑148 (MQ=255) cgTCTGGCAGTTGCTTGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAGGCCGGCGCAg < 5:54171/149‑1 (MQ=255) tCTGGCAGTTGCTTGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTg > 7:215757/1‑149 (MQ=255) cTGGCAGTTGCTTGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTg > 7:336388/1‑148 (MQ=255) ttGCTTGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGc > 8:266863/1‑148 (MQ=255) gCTTGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCa < 7:129579/148‑1 (MQ=255) gCTTGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAg > 6:213624/1‑149 (MQ=255) cTTGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGcagta > 1:370518/1‑145 (MQ=255) cTTGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAg < 7:131797/148‑1 (MQ=255) gAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTAtt > 3:175063/1‑149 (MQ=255) gCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTcc > 8:338059/1‑149 (MQ=255) cAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTcc‑‑c < 4:175063/149‑1 (MQ=255) aGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACt > 1:226193/1‑136 (MQ=255) aGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACt < 2:226193/136‑1 (MQ=255) ggCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCActgctg < 5:99842/110‑1 (MQ=255) ggCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCActgctg > 6:99842/1‑110 (MQ=255) ggCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCC‑‑CCgg > 5:66382/1‑149 (MQ=255) cccTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCC‑‑CCGG‑Ca < 2:372486/149‑1 (MQ=255) ccTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGc > 7:185189/1‑127 (MQ=255) ccTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGc < 8:185189/127‑1 (MQ=255) ccTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCC‑‑CCGG‑CAc > 1:197162/1‑149 (MQ=255) gATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCC‑‑CCGG‑CACTGCTGTACt < 7:338059/149‑1 (MQ=255) aTCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCC‑‑CCGG‑CACTGCTGTACTg < 7:266863/149‑1 (MQ=255) cAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCCGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCC‑‑CCGG‑CActg > 4:272768/1‑139 (MQ=255) cAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCCGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCC‑‑CCGG‑CActg < 3:272768/139‑1 (MQ=255) aGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCC‑‑CCGG‑CACTGCTGTACTGCa < 8:215757/148‑1 (MQ=255) aGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCC‑‑CCGG‑CACTGCTGTACTGCa < 4:287013/148‑1 (MQ=255) aGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAGGCCAGCGCAGTGCAACTGCCAGTATTCC‑‑CCGG‑CACTGCTGTACTGCa < 6:90317/148‑1 (MQ=255) ggCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCC‑‑CCGG‑CACTGCTGTACTGCAGtt > 8:116536/1‑149 (MQ=255) cGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCC‑‑CCGG‑CACTGCTGTACTGCAGTTGCa < 2:197162/149‑1 (MQ=255) gggggCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCC‑‑CCGG‑CACTGCTGTACTGCAGTTGCAc < 7:31289/149‑1 (MQ=255) ggggCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCC‑‑CCGG‑CACTGCTGTACTGCAGTTGCACg > 8:43552/1‑149 (MQ=255) ggggCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCC‑‑CCGG‑CACTGCAGTACAGCAGtgccggg < 8:336388/149‑7 (MQ=255) gggCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCC‑‑CCGG‑CACTGCTGTACTGCAGTTGCACg > 2:107396/1‑148 (MQ=255) gCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCC‑‑CCGG‑CACTGCTGTACTGCAGTTGCACGGAg < 6:66382/149‑1 (MQ=255) cGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCC‑‑CCGG‑CACTGCTGTACTGCAGTTGCACGGAGGCGCGGt > 2:324887/1‑149 (MQ=255) gTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCC‑‑CCGG‑CACTGCTGTACTGCAGTTGCACGGAGGCGCGGt > 6:82845/1‑148 (MQ=255) gCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCC‑‑CCGG‑CACTGCTGTACTGCAGTTGCACGGAGGCGCGGTcac < 6:354278/149‑1 (MQ=255) gCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCC‑‑CCGG‑CACTGCTGTACTGCAGTTGCACGGAGGCGCGGTca > 6:100717/1‑148 (MQ=255) cAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCC‑‑CCGG‑CACTGCTGTACTGCAGTTGCACGGAGGCGCGGTcac > 6:128159/1‑148 (MQ=255) cAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCC‑‑CCGG‑CACTGCTGTACTGCAGTTGCACGGAGGCGCGGTcac < 4:39102/148‑1 (MQ=255) gCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCC‑‑CCGG‑CACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACGt > 3:84952/1‑149 (MQ=255) gCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCC‑‑CCGG‑CACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACGt > 7:380160/1‑149 (MQ=255) agccagcAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCC‑‑CCGG‑CACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACGTTc < 7:43552/149‑1 (MQ=255) agccagcAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCC‑‑CCGG‑CACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACGTTc < 5:34929/149‑1 (MQ=255) cagcAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCC‑‑CCGG‑CACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACGTTCCTg < 5:82845/149‑1 (MQ=255) gTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCC‑‑CCGG‑CACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACGTTCCTGCAGTGGCGCCAgg > 3:346952/1‑149 (MQ=255) gcgtGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCC‑‑CCGG‑CACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACGTTCCTGCAGTGGCGCCAGGGc < 3:304542/145‑1 (MQ=255) gCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCC‑‑CCGG‑CACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACGTTCCTGCAGTGGCGCCAGGGc < 6:188815/148‑1 (MQ=255) gCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCC‑‑CCGG‑CACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACGTTCCTGCAGTGGCGCCAGGGc < 5:128159/148‑1 (MQ=255) aGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCC‑‑CCGG‑CACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACGTTCCTGCAGTGGCGCCAGGGCCTGTTc < 5:100717/149‑1 (MQ=255) gCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCC‑‑CCGG‑CACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACGTTCCTGCAGTGGCGCCAGGGCCTGTTcc < 5:213624/149‑1 (MQ=255) aTCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCC‑‑CCGG‑CACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACGTTCCTGCAGTGGCGCCAGGGCCTGTTCCAg < 1:178619/149‑1 (MQ=255) tCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCC‑‑CCGG‑CACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACGTTCCTGCAGTGGCGCCAGGGCCTGTTCCAGc < 8:36800/149‑1 (MQ=255) tCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCC‑‑CCGG‑CACTGCAGTACTGCAGTTGCACGGAGGCGCGGTCACAACGTTCCTGCAGTGGCGCCAGGGCCTGTTCCAGc > 3:192714/1‑149 (MQ=255) tCAAGGCCAAGGCTTGTTCCACTGCCAGCAGCACTGCAGCTGGCAGGCCCGCGCACTCCAACAGCCAGTAATCC‑‑CCCG‑CACTGCTGAACAGCAGTAGCACGGAGGCGCGGTCACAACGTTCCTCCAGTGGCGCCACGGCCTGTTCCAg > 1:258654/1‑148 (MQ=255) aaggccaaggcTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCC‑‑CCGG‑CACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACGTTCCTGCAGTGGCGCCAGGGCCTGTTCCAGca > 6:53629/1‑148 (MQ=255) caaggcTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCCTGGAGGACAATGGTTTACGGCAGTTGCCCGGAGGCGCGGACAAAACTTGCGGGCAGTGCCCCCAGGGCATGTTccgccaccc > 1:125880/1‑142 (MQ=255) caaggcTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCC‑‑CCGG‑CACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACGTTCCTGCAGTGGCGCCAGGGCCTGTTCCAGCACACGCt > 3:27327/1‑149 (MQ=255) caaggcTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCC‑‑CCGG‑CACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACGTTCCTGCAGTGGCGCCAGGGCCTGTTCCAGCACACGCt > 6:412677/1‑149 (MQ=255) caaggcTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCC‑‑CCGG‑CACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACGTTCCTGCAGT‑GCGCCAGGGCCTGTTCCAGCACACGCTg > 6:94290/1‑149 (MQ=255) aaggcTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCC‑‑CCGG‑CACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACGTTCCTGCAGTGGCGCCAGGGCCTGTTCCAGCACACGCTg > 1:249113/1‑149 (MQ=255) gcTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCC‑‑CCGG‑CACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACGTTCCTGCAGTGGCGCCAGGGCCTGTTCCAGCACACGCTGTaa < 4:346952/149‑1 (MQ=255) cTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCC‑‑CCGG‑CACTGCTGTACTGCAGTTGCACGGAGGCGCAGTCACAACGTTCCTGCAGTGGCGCCAGGGCCTGTTCCAGCACACGCTGTAAc > 7:101691/1‑149 (MQ=255) cccGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCC‑‑CCGG‑CACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACGTTCCTGCAGTGGCGCCAGGGCCTGTTCCAGCACACGCTGTAACGGCTGGCGCAt < 2:249113/148‑1 (MQ=255) cccGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTAC‑‑CCGG‑CACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACGTTCCTGCAGTGGCGCCAGGGCCTGTTCCAGCACACGCTGTAACGGCTGgcg > 4:225993/1‑145 (MQ=255) ccagcagcaGTGCTGCGGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCC‑‑CCGG‑CACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACGTTCCTGCAGTGGCGCCAGGGCCTGTTCCAGCACACGCTGTAACGGCTGGCGCa > 5:295987/4‑147 (MQ=255) ccagcagcaGTGCTGCGGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCC‑‑CCGG‑CACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACGTTCCTGCAGTGGCGCCAGGGCCTGTTCCAGCACACGCTGTAACGGCTGGCGCa > 5:377325/4‑147 (MQ=255) ccGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCC‑‑CCGG‑CActgctg > 5:196436/1‑61 (MQ=255) ccGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCC‑‑CCGG‑CActgctg < 6:196436/61‑1 (MQ=255) agcaGTGCTGCGGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCC‑‑CCGG‑CACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACGTTCCTGCAGTGGCGCCAGGGCCTGTTCCAGCACACGCTGTAACTGCTGGCGCATAGGTGa < 6:377325/149‑1 (MQ=255) | GGCAGGCTACCCCGTACCGCGTCTGGCAGTTGCTTGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCC‑‑CCGG‑CACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACGTTCCTGCAGTGGCGCCAGGGCCTGTTCCAGCACACGCTGTAACGGCTGGCGCATAGGTGA > NC_002947/460885‑461174 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |