Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,640,106 C→A 5.4% Q400K (CAG→AAG)  PP_4950 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,640,1060CA 5.4% 240.4 / 3.0 74Q400K (CAG→AAG) PP_4950hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (35/34);  new base A (2/2);  total (38/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.79e-01

GGTACCTGAACTACTCAGACCTGCCAGCGGCGCGCATGACCGGGCTGACCCGCTTCGCCACTTTCTGGGTAGAAGACGGGCAGATCCAGGGGCCGGTGAGTACCATGCGCTTCGATGACAGCTTGTACAGCTTGCTGGGCAGCCAGCTGGAGGACCTGACCCAGGAGCGCGAGATGATCCTGTCGACCAGTACTTATGGACAGCGCAGTACCGGGTCGAGTCATTTGCCCGGGGCACTGGTCAAAGGCCTGACCCTGACATTGTGACTGGACTTGCCGGCCCTTTCGCGGC  >  NC_002947/5639963‑5640253
                                                                                                                                               |                                                                                                                                                   
ggTACCTGAACTACTCAGACCTGCCAGCGGCGCGCATGACCGGGCTGACCCGCTTCGCCACTTTCTGGGTAGAAGACGGGCAGATCCAGGGGCCGGTGAGTACCATGCGCTTCGATGACAGCTTGTACAGCTTGCTGGGCAGCCAGCTg                                                                                                                                                >  7:40436/1‑149 (MQ=255)
 gTACCTGAACTACTCAGACCTGCCAGCGGCGCGCATGACCGGGCTGACCCGCTTCGCCACTTTCTGGGTAGAAGACGGGCAGATCCAGGGGCCGGTGAGTACCATGCGCTTCGATGACAGCTTGTACAGCTTGCTGGGCAGCCAGCTg                                                                                                                                                <  3:217700/148‑1 (MQ=255)
    ccTGAACTACTCAGACCTGCCAGCGGCGCGCATGACCGGGCTGACCCGCTTCGCCACTTTCTGGGTAGAAGACGGGCAGATCCAGGGGCCGGTGAGTACCATGCGCTTCGATGACAGCTTGTACAGCTTGCTGGGCAGCCAGCTggag                                                                                                                                             <  3:308622/148‑1 (MQ=255)
      tGAACTACTCAGACCTGCCAGCGGCGCGCATGACCGGGCTGACCCGCTTCGCCAATTTCTGGGTAGAAGACGGGCAGATCCAGGGGCCGGTGAGTACCATGCGCTTCGATGACAGCTTGTACAGCTTGCTGGGCAGCCAGCTGGAGGAc                                                                                                                                          >  1:310815/1‑149 (MQ=255)
        aaCTACTCAGACCTGCCAGCGGCGCGCATGACCGGGCTGACCCGCTTCGCCACTTTCTGGGTAGAAGACGGGCAGATCCAGGGGCCGGTGAGTACCATGCGCTTCGATGACAGCTTGTACAGCTTGCTGGGCAGCCAGCTGGAGGAc                                                                                                                                          <  2:319246/147‑1 (MQ=255)
        aaCTACTCAGACCTGCCAGCGGCGCGCATGACCGGGCTGACCCGCTTCGCCACTTTCTGGGTAGAAGACGGGCAGATCCAGGGGCCGGTGAGTACCATGCGCTTCGATGACAGCTTGTACAGCTTGCTGGGCAGCCAGCTGGAGGACCt                                                                                                                                        >  4:252894/1‑149 (MQ=255)
          ctactCAGACCTGCCAGCGGCGCGCATGACCGGGCTGACCCGCTTCGCCACTTTCTGGGTAGAAGACGGGCAGATCCAGGGGCCGGTGAGTACCATGCGCTTCGATGACAGCTTGTACAGCTTGCTGGGCAGCCAGCTGGAGGACCTGa                                                                                                                                      <  4:196916/149‑1 (MQ=255)
             ctCAGACCTGCCAGCGGCGCGCATGACCGGGCTGACCCGCTTCGCCACTTTCTGGGTAGAAGACGGGCAGATCCAGGGGCCGGTGAGTACCATGCGCTTCGATGACAGCTTGTACAGCTTGCTGGGCAGCCAGCTGGAGGACCTGAccc                                                                                                                                   <  6:266204/149‑1 (MQ=255)
             ctCAGACCTGCCAGCGGCGCGCATGACCGGGCTGACCCGCTTCGCCACTTTCTGGGTAGAAGACGGGCAGATCCAGGGGCCGGTGAGTACCATGCGCTTCGATGACAGCTTGTACAGCTTGCTGGGCAGCCAGCTGGAGGACCTGAcc                                                                                                                                    >  6:180066/1‑148 (MQ=255)
             ctCAGACCTGCCAGCGGCGCGCATGACCGGGCTGACCCGCTTCGCCACTTTCTGGGTAGAAGACGGGCAGATCCAGGGGCCGGTGAGTACCATGCGCTTCGATGACAGCTTGTACAGCTTGCTGGGCAGCCAGCTGGAGGACCTGAcc                                                                                                                                    >  6:324537/1‑148 (MQ=255)
                aGACCTGCCAGCGGCGCGCATGACCGGGCTGACCCGCTTCGCCACTTTCTGGGTAGAAGACGGGCAGATCCAGGGGCCGGTGAGTACCATGCGCTTCGATGACAGCTTGTACAGCTTGCTGGGCAGCCAGCTGGAGGACCTGACCCAg                                                                                                                                 >  1:233311/1‑148 (MQ=255)
                   ccTGCCAGCGGCGCGCATGACCGGGCTGACCCGCTTCGCCACTTTCTGGGTAGAAGACGGGCAGATCCAGGGGCCGGTGAGTACCATGCGCTTCGATGACAGCTTGTACAGCTTGCTGGGCAGCCAGCTGGAGGACCTGACCCAGGAgc                                                                                                                             >  8:146322/1‑149 (MQ=255)
                       ccAGCGGCGCGCATGACCGGGCTGACCCGCTTCGCCACTTTCTGGGTAGAAGACGGGCAGATCCAGGGGCCGGTGAGTACCATGCGCTTCGATGACAGCTTGTACAGCTTGCTGGGCAGCCAGCTGGAGGACCTGACCCAGGAgcgc                                                                                                                           >  3:334479/1‑147 (MQ=255)
                              cgcgcATGACCGGGCTGACCCGCTTCGCCACTTTCTGGGTAGAAGACGGGCAGATCCAGGGGCCGGTGAGTACCATGCGCTTCGATGACAGCTTGTACAGCTTGCTGGGCAGCCAGCTGGAGGACCTGACCCAGGAGCGCGAGATGATc                                                                                                                  <  5:147307/149‑1 (MQ=255)
                                  cATGACCGGGCTGACCCGCTTCGCCACTTTCTGGGTAGAAGACGGGCAGATCCAGGGGCCGGTGAGTACCATGCGCTTCGATGACAGCTTGTACAGCTTGCTGGGCAGCCAGCTGGAGGACCTGACCCAGGAGCGCGAGATGATCCTGt                                                                                                              <  2:233311/149‑1 (MQ=255)
                                     gACCGGGCTGACCCGCTTCGCCACTTTCTGGGTAGAAGACGGGCAGATCCAGGGGCCGGTGAGTACCATGCGCTTCGATGACAGCTTGTACAGCTTGCTGGGCAGCCAGCTGGAGGACCTGACCCAGGAGCGCGAGATGATc                                                                                                                  >  8:98418/1‑142 (MQ=255)
                                     gACCGGGCTGACCCGCTTCGCCACTTTCTGGGTAGAAGACGGGCAGATCCAGGGGCCGGTGAGTACCATGCGCTTCGATGACAGCTTGTACAGCTTGCTGGGCAGCCAGCTGGAGGACCTGACCCAGGAGCGCGAGATGATc                                                                                                                  <  7:98418/142‑1 (MQ=255)
                                      aCCGGGCTGACCCGCTTCGCCACTTTCTGGGTAGAAGACGGGCAGATCCAGGGGCCGGTGAGTACCATGCGCTTCGATGACAGCTTGTACAGCTTGCTGGGCAGCCAGCTGGAGGACCTGACCCAGGAGCGCgaga                                                                                                                       >  2:82744/1‑136 (MQ=255)
                                      aCCGGGCTGACCCGCTTCGCCACTTTCTGGGTAGAAGACGGGCAGATCCAGGGGCCGGTGAGTACCATGCGCTTCGATGACAGCTTGTACAGCTTGCTGGGCAGCCAGCTGGAGGACCTGACCCAGGAGCGCGAGATGATCCTGTCGAc                                                                                                          <  3:252894/149‑1 (MQ=255)
                                      aCCGGGCTGACCCGCTTCGCCACTTTCTGGGTAGAAGACGGGCAGATCCAGGGGCCGGTGAGTACCATGCGCTTCGATGACAGCTTGTACAGCTTGCTGGGCAGCCAGCTGGAGGACCTGACCCAGGAGCGCGAGATGATCCTGTCGAc                                                                                                          <  7:146322/149‑1 (MQ=255)
                                      aCCGGGCTGACCCGCTTCGCCACTTTCTGGGTAGAAGACGGGCAGATCCAGGGGCCGGTGAGTACCATGCGCTTCGATGACAGCTTGTACACCTTGCTGGGCAGCCAGCTGGAGGACCTGACCCAGGAGCGCgaga                                                                                                                       <  1:82744/136‑1 (MQ=255)
                                          ggCTGACCCGCTTCGCCACTTTCTGGGTAGAAGACGGGCAGATCCAGGGGCCGGTGAGTACCATGCGCTTCGATGACAGCTTGTACAGCTTGCTGGGCAGCCAGCTGGAGGACCTGACCCAGGAGCGCGAGATGATCCTGTCGACCAg                                                                                                       >  2:1204/1‑148 (MQ=255)
                                                 ccGCTTCGCCACTTTCTGGGTAGAAGACGGGCAGATCCAGGGGCCGGTGAGTACCTTGCGCTTCGATGACAGCTTGTACAGCTTGCTGGGCAGCCAGCTGGAGGACCTGACCCAGGCGCTCGAGATGATCCTGTCGACCAGTACTTATg                                                                                               >  4:267048/1‑149 (MQ=255)
                                                   gCTTCGCCACTTTCTGGGTAGAAGACGGGCAGATCCAGGGGCCGGTGAGTACCATGCGCTTCGATGACAGCTTGTACAGCTTGCTGGGCAGCCAGCTGGAGGAc                                                                                                                                          >  2:367032/1‑104 (MQ=255)
                                                   gCTTCGCCACTTTCTGGGTAGAAGACGGGCAGATCCAGGGGCCGGTGAGTACCATGCGCTTCGATGACAGCTTGTACAGCTTGCTGGGCAGCCAGCTGGAGGAc                                                                                                                                          <  1:367032/104‑1 (MQ=255)
                                                     ttCGCCACTTTCTGGGTAGAAGACGGGAAGATCCAGGGGCCGGTGAGTACCATGCGCTTCGATGACAGCTTGTACAGCTTGCTGGGCAGCCAGCTGGAGGACCTGACCCAGGAGCGCGAGATGATCCTGTCGACCAGTACTTATGGACa                                                                                           <  3:29736/149‑1 (MQ=255)
                                                        tccACTTTCAGGGTAGAAGACGGGCAGATCCAGGGGCCGGTGAGTACCATGAGCTTCGATGACAGCTAGTACAGCTTGATTCGCAGCGAGCTGGAGGACCTGACCCAGGAGCGCGAGAAGATCCTGTCGAGCAGTACATATGGACcgcg                                                                                        >  8:154311/2‑149 (MQ=255)
                                                        gCCACTTTCTGGGTAGAAGACGGGCAGATCCAGGGGCCGGTGAGTACCATGCGCTTCGATGACAGCTTGTACAGCTTGCTGGGCAGCCAGCTGGAGGACCTGACCCAGGAGCGCGAGATGATc                                                                                                                  >  8:241710/1‑123 (MQ=255)
                                                        gCCACTTTCTGGGTAGAAGACGGGCAGATCCAGGGGCCGGTGAGTACCATGCGCTTCGATGACAGCTTGTACAGCTTGCTGGGCAGCCAGCTGGAGGACCTGACCCAGGAGCGCGAGATGATc                                                                                                                  <  7:241710/123‑1 (MQ=255)
                                                        gCCACTTTCTGGGTAGAAGACGGGCAGATCCAGGGGCCGGTGAGTACCATGCGCTTCGATGACAGCTTGTACAGCTTGCTGGGCAGCCAGCTGGAGGACCTGACCCAGGAGCGCGAGATGATCCTGTCGACCAGTACTTATGGACAgca                                                                                        >  8:187652/1‑148 (MQ=255)
                                                         ccACTTTCTGGGTAGAAGACGGGCAGATCCAGGGGCCGGTGAGTACCATGCGCTTCGATGACAGCTTGTACAGCTTGCTGGGCAGCCAGCTGGAGGACCTGACCCAGGAGCGCGAGATGATCCTGTCGACCAGTACTTATGGACAgcg                                                                                        >  3:357042/1‑148 (MQ=255)
                                                               tCTGGGTAGAAGACGGGCAGATCCAGGGGCCGGTGAGTACCATGCGCTTCGATGACAGCTTGTACAGCTTGCTGGGCAGCCAGCTGGAGGACCTGACCCAGGAGCGCGAGATGATCCTGTCGACCAGTACTTATGGACAGCGCAGTAcc                                                                                 >  8:42451/1‑149 (MQ=255)
                                                                cTGGGTAGAAGACGGGCAGATCCAGGGGCCGGTGAGTACCATGCGCTTCGATGACAGCTTGTACAGCTTGCTGGGCAGCCAGCTGGAGGACCTGACCCAGGAGCGCGAGATGATCCTGTCGACCAGTACTTATGGACAGCGCAGTAcc                                                                                 <  2:310815/148‑1 (MQ=255)
                                                                   ggTAGAAGACGGGCAGATCCAGGGGCCGGTGAGTACCATGCGCTTCGATGACAGCTTGTACAGCTTGCTGGGCAGCCAGCTGGAGGACCTGACCCAGGAGCGCGAGATGATCCTGTCGACCAGTACTTATGGACAGCGCAGTACCGGGt                                                                             >  7:182790/1‑149 (MQ=255)
                                                                       gaagaCGGGCAGATCCAGGGGCCGGTGAGTACCATGCGCTTCGATGACAGCTTGTACAGCTTGCTGGGCAGCCAGCTGGAGGACCTGACCCAGGAGCGCGAGATGATCCTGTCGACCAGTACTTATGGACAGCGCAGTACCGGGTCGa                                                                          <  1:1204/148‑1 (MQ=255)
                                                                             gggCAGATCCAGGGGCCGGTGAGTACCATGCGCTTCGATGACAGCTTGTACAGCTTGCTGGGCAGCCAGCTGGAGGACCTGACCCAGGAGCGCGAGATGATCCTGTCGACCAGTACTTATGGACAGCGCAGTACCGGGTCGAGTCAttt                                                                   >  1:63493/1‑149 (MQ=255)
                                                                                     ccAGGGGCCGGTGAGTACCATGCGCTTCGATGACAGCTTGTACAGCTTGCTGGGCAGCCAGCTGGAGGACCTGACCCAGGAGCGCGAGATGATCCTGTCGACCAGTACTTATGGACAGCGCAGTACCGGGTCGAGTCATTTGCCCgggg                                                           >  3:15546/1‑149 (MQ=255)
                                                                                     ccAGGGGCCGGTGAGTACCATGCGCTTCGATGACAGCTTGTACAGCTTGCTGGGCAGCCAGCTGGAGGACCTGACCCAGGAGCGCGAGATGATCCTGTCGACCAGTACTTATGGACAGCGCAGTACCGGGTCGAGTCATTTGCCCggg                                                            <  2:63493/148‑1 (MQ=255)
                                                                                      cAGGGGCCGGTGAGTACCATGCGCTTCGATGACAGCTTGTACAGCTTGCTGGGCAGCCAGCTGGAGGACCTGACCCAGGAGCGCGAGATGATCCTGTCGACCAGTACTTATGGACAGCGCAGTACCGGGTCGAGTCATTTGCCCgggg                                                           <  5:180066/148‑1 (MQ=255)
                                                                                       aGGGGCCGGTGAGTACCATGCGCTTCGATGACAGCTTGTACAGCTTGCTGGGCAGCCAGCTGGAGGACCTGACCCAGGAGCGCGAGATGATCCTGTCGACCAGTACTTATGGACAGCGCAGTACCGGGTCGAGTCATTTGCCCGGGGCa                                                         >  5:76425/1‑149 (MQ=255)
                                                                                                  aGTACCATGCGCTTCGATGACAGCTTGTACAGCTTGCTGGGCAGCCAGCTGGAGGACCTGACCCAGGAGCGCGAGATGATCCTGTCGACCAGTACTTATGGACAGCGCAGTACCGGGTCGAGTCATTTGCCCGGGGCACTGGTCAAAgg                                              >  2:328125/1‑149 (MQ=255)
                                                                                                  aGTACCATGCGCTTCGATGACAGCTTGTACAGCTTGCTGGGCAGCCAGCTGGAGGACCTGACCCAGGAGCGCGAGATGATCCTGTCGACCAGTACTTATGGACAGCGCAGTACCGGGTCGAGTCATTTGCCCGGGGCACTGGTCAAAg                                               <  4:357042/148‑1 (MQ=255)
                                                                                                  aGTACCATGCGCTTCGATGACAGCTTGTACAGCTTGCTGGGCAGCCAGCTGGAGGACCTGACCCAGGAGCGCGAGATGATCCTGTCGACCAGTACTTATGGACAGCGCAGTACCGGGTCGAGTCATTTGCCCGGGGCACTGGTCAAAg                                               <  8:40436/148‑1 (MQ=255)
                                                                                                   gTACCATGCGCTTCGATGACAGCTTGTACAGCTTGCTGGGCAGCCAGCTGGAGGACCTGACCCAGGAGCGCGAGATGATCCTGTCGACCAGTACTTATGGACAGCGCAGTACCGGGTCGAGTCATTTGCCCGGGGCACTGGTCAAAGGc                                             <  4:334479/149‑1 (MQ=255)
                                                                                                      ccATGCGCTTCGATGACAGCTTGTACAGCTTGCTGGGCAGCCAGCTGGAGGACCTGACCCAGGAGCGCGAGATGATCCTGTCGACCAGTACTTATGGACAGCGCAGTACCGGGTCGAGTCATTTGCCCGGGGCACTGGTCAAAGGcct                                           >  2:57963/1‑148 (MQ=255)
                                                                                                         tGCGCTTCGATGACAGCTTGTACAGCTTGCTGGGCAGCCAGCTGGAGGACCTGACCCAGGAGCGCGAGATGATCCTGTCGACCAGTACTTATGGACAGCGCAGTACCGGGTCGAGTCATTTGCCCGGGGCACTGGTCAAAGGcctgacc                                       <  7:42451/149‑1 (MQ=255)
                                                                                                               tCGATGACAGCTTGTACAGCTTGCTGGGCAGCCAGCTGGAGGACCTGACCCAGGAGCGCGAGATGATCCTGTCGACCAGTACTTATGGACAGCGCAGTACCgg                                                                               >  1:153001/1‑103 (MQ=255)
                                                                                                               tCGATGACAGCTTGTACAGCTTGCTGGGCAGCCAGCTGGAGGACCTGACCCAGGAGCGCGAGATGATCCTGTCGACCAGTACTTATGGACAGCGCAGTACCgg                                                                               <  2:153001/103‑1 (MQ=255)
                                                                                                                  aTGACAGCTTGTACAGCTTGCTGGGCAGCCAGCTGGAGGACCTGACCCAGGAGCGCGAGATGATCCTGTCGACCAGTACTTATGGACAGCGCAGTACCGGGTCGAGTCATTTGCCCGGGGCACTGGTCAAAGGCCTGACCCTGACATtg                              <  1:328125/149‑1 (MQ=255)
                                                                                                                   tGACAGCTTGTACAGCTTGCTGGGCAGCCAGCTGGAGGACCTGACCCAGGAGCGCGAGATGATCCTGTCGACCAGTACTTATGGACAGCGCAGTACCGGGTCGAGTCATTTGCCCGGGGCACTGGTCAAAGGCCTGACCCTGACATtgt                             <  6:76425/149‑1 (MQ=255)
                                                                                                                     aCAGCTTGTACAGCTTGCTGGGCAGCCAGCTGGAGGACCTGACCCAGGAGCGCGAGATGATCCTGTCGACCAGTACTTATGGACAGCGCAGTACCGGGTCGAGTCTTTTGCCCGGGGCACTGGTCAAAGGCCTGACCCTGACATTGTGa                           >  7:72911/1‑149 (MQ=255)
                                                                                                                             tccAGCTGGCTGCCCAGCAAGCTGGAGGACCTGACCCAGGAGCGCGAGATGATCCTGTCGACCAGTACTTATGGACAGCGCAGTACCGGGTCGAGTCATTTGCCCGGGGCACTGGTCAAAGGcctgaccct                                     >  7:137182/3‑131 (MQ=255)
                                                                                                                             tccAGCTGGCTGCCCAGCAAGCTGGAGGACCTGACCCAGGAGCGCGAGATGATCCTGTCGACCAGTACTTATGGACAGCGCAGTACCGGGTCGAGTCATTTGCCCGGGGCACTGGTCAAAGGcctgaccct                                     <  8:137182/129‑1 (MQ=255)
                                                                                                                             tccAGCTGGCTGCCCAGCAAGCTGGAGGACCTGACCCAGGAGCGCGAGATGATCCTGTCGACCAGTACTTATGGACAGCGCAGTACCGGGTCGAGTCATTTGCCCGGGGCACTGGTCAAAGGcctgaccct                                     <  8:317353/129‑1 (MQ=255)
                                                                                                                             tccAGCTGGCTGCCCAGCAAGCTGGAGGACCTGACCCAGGAGCGCGAGATGATCCTGTCGACCAGTACTTATGGACAGCGCAGTACCGGGTCGAGTCATTTGCCCGGGGCACTGGTCAAAGGcctgaccct                                     >  7:317353/3‑131 (MQ=255)
                                                                                                                               cAGCTTGCTGGGCAGCCAGCTGGAGGACCTGACCCAGGAGCGCGAGATGATCCTGTCGACCAGTACTTATGGACAGCGCAGTACCGGGTCGAGTCATTTGCCCGGGGCACTGGTCAAAGGCCTGACCCTGACATTGTGACTGGACTTGc                 >  7:16427/1‑149 (MQ=255)
                                                                                                                                aGCTTGCTGGGCAGCCAGCTGGAGGACCTGACCCAGGAGCGCGAGATGATCCt                                                                                                                >  5:79518/1‑53 (MQ=255)
                                                                                                                                aGCTTGCTGGGCAGCCAGCTGGAGGACCTGACCCAGGAGCGCGAGATGATCCt                                                                                                                <  6:79518/53‑1 (MQ=255)
                                                                                                                                aGCTTGCTGGGCAGCCAGCTGGAGGACCTGACCCAGGAGCGCGAGATGATCCTGTCGACCAGTACTTATGGACAGCGCAGTACCGGGTCGAGTCATTTGCCCGGGGCACTGGTCAAAGGCCTGACCCTGACATTGTGACTGGACTTGcc                >  7:362547/1‑149 (MQ=255)
                                                                                                                                  cTTGCTGGGCAGCCAGCTGGAGGACCTGACCCAGGAGCGCGAGATGATCCTGTCGACCAGTACTTATGGACAGCGCAGTACCGGGTCGAGTCATTTGCCCGGGGCACTGGTCAAAGGCCTGACCCTGACATTGTGACTGGACTTGCCgg              <  8:72911/149‑1 (MQ=255)
                                                                                                                                  cTTGCTGGGCAGCCAGCTGGAGGACCTGACCCAGGAGCGCGAGATGATCCTGTCGACCAGTACTTATGGACAGCGCAGTACCGGGTCGAGTCATTTGCCCGGGGCACTGGTCAAAGGCCTGACCCTGAAATTGTGACTGGACTTGCCg               <  8:362547/148‑1 (MQ=255)
                                                                                                                                   ttGCTGGGCAGCCAGCTGGAGGACCTGACCCAGGAGCGCGAGATGATCCTGTCGACCAGTACTTATGGACAGCGCAGTACCGGGTCGAGTCATTTGCCCGGGGCACTGGTCAAAg                                               <  6:97915/115‑1 (MQ=255)
                                                                                                                                   ttGCTGGGCAGCCAGCTGGAGGACCTGACCCAGGAGCGCGAGATGATCCTGTCGACCAGTACTTATGGACAGCGCAGTACCGGGTCGAGTCATTTGCCCGGGGCACTGGTCAAAg                                               >  5:97915/1‑115 (MQ=255)
                                                                                                                                   ttGCTGGGCAGCCAGCTGGAGGACCTGACCCAGGAGCGCGAGATGATCCTGTCGACCAGTACTTATGGACAGCGCAGTACCGGGTCGAGTCATTTGCCCGGGGCACTGGTCAAAGGCCTGACCCTGACATTGTGACTGGACTTGCCGGc             >  5:218853/1‑149 (MQ=255)
                                                                                                                                     gCTGGGCAGCCAGCTGGAGGACCTGACCCAGGAGCGCGAGATGATCCTGTCGACCAGTACTTATGGACAGCGCAGTACCGGGTCGAGTCATTTGCCCGGGGCACTGGTCAAAGGCCTGACCCTGACATTGTGACTGGACTTGCCGGcc            <  4:15546/148‑1 (MQ=255)
                                                                                                                                      cTGGGCAGCCAGCTGGAGGACCTGACCCAGGAGCGCGAGATGATCCTGTCGACCAGTACTTATGGACAGCGCAGTACCGGGTCGAGTCATTTGCCCGGGGCACTGGTCaaa                                                >  8:376004/1‑111 (MQ=255)
                                                                                                                                      cTGGGCAGCCAGCTGGAGGACCTGACCCAGGAGCGCGAGATGATCCTGTCGACCAGTACTTATGGACAGCGCAGTACCGGGTCGAGTCATTTGCCCGGGGCACTGGTCaaa                                                <  7:376004/111‑1 (MQ=255)
                                                                                                                                       tGGGCAGCCAGCTGGAGGACCTGACCCAGGAGCGCGAGATGATCCTGTCGACCAGTACTTATGGACAGCGCAGTACCGGGTCGAGTCATTTGCCCGGGGCACTGGTCAAAGGcctgac                                        >  7:140557/1‑118 (MQ=255)
                                                                                                                                       tGGGCAGCCAGCTGGAGGACCTGACCCAGGAGCGCGAGATGATCCTGTCGACCAGTACTTATGGACAGCGCAGTACCGGGTCGAGTCATTTGCCCGGGGCACTGGTCAAAGGcctgac                                        <  8:140557/118‑1 (MQ=255)
                                                                                                                                        gggCAGCCAGCTGGAGGACCTGACCCAGGAGCGCGAGATGATCCTGTCGACCAGTACTTATGGACAGCGCAGTACCGGGTCGAGTCATTTGCCCGGGGCACTGGTCAAAGGCCTGACCCTGACATTGTGACTGGACTTGCCGGCCCttt        >  5:329722/1‑149 (MQ=255)
                                                                                                                                         ggCAGCCAGCTGGAGGACCTGACCCAGGAGCGCGAGATGATCCTGTCGACCAGTACTTATGGACAGCGCAGTACCGGGTCGAGTCATTTGCCCGGGGCACTGGTCAAAGGCCTGACCCTGACATtg                              >  5:325318/1‑126 (MQ=255)
                                                                                                                                         ggCAGCCAGCTGGAGGACCTGACCCAGGAGCGCGAGATGATCCTGTCGACCAGTACTTATGGACAGCGCAGTACCGGGTCGAGTCATTTGCCCGGGGCACTGGTCAAAGGCCTGACCCTGACATtg                              <  6:325318/126‑1 (MQ=255)
                                                                                                                                          gCAGCCAGCTGGAGGACCTGACCCAGGAGCGCGAGATGATCCTGTCGACCAGTACTTATGGACAGCGCAGTACCGGGTCGAGTCATTTGCCCGGGGCACTGGTCAAAGGCCTGACCCTGACATtgt                             <  6:43626/126‑1 (MQ=255)
                                                                                                                                          gCAGCCAGCTGGAGGACCTGACCCAGGAGCGCGAGATGATCCTGTCGACCAGTACTTATGGACAGCGCAGTACCGGGTCGAGTCATTTGCCCGGGGCACTGGTCAAAGGCCTGACCCTGACATtgt                             >  5:43626/1‑126 (MQ=255)
                                                                                                                                             gccagcTGGAGGACCTGACCCAGGAGCGCGAGATGATCCTGTCGACCAGTACTTATGGACAGCACAGTACCGGGTCGAGTCATTTGCCCGGGGCACTGGTCAAAGGCCTGACCCTGACATTGTGACTGGACTTGCCGGCCCTTTCGCgg   <  7:187652/149‑1 (MQ=255)
                                                                                                                                               cagcTGGAGGACCTGACCCAGGAGCGCGAGATGATCCTGTCGACCAGTACTTATGGACAGCGCAGTACCGGGTCGAGTCATTTGCCCGGGGCACTGGTCAAAGGCCTGACCCTGACATTGTGACTGGACTTGCCGGCCCTTTCGCGGc  >  3:124726/1‑148 (MQ=255)
                                                                                                                                               |                                                                                                                                                   
GGTACCTGAACTACTCAGACCTGCCAGCGGCGCGCATGACCGGGCTGACCCGCTTCGCCACTTTCTGGGTAGAAGACGGGCAGATCCAGGGGCCGGTGAGTACCATGCGCTTCGATGACAGCTTGTACAGCTTGCTGGGCAGCCAGCTGGAGGACCTGACCCAGGAGCGCGAGATGATCCTGTCGACCAGTACTTATGGACAGCGCAGTACCGGGTCGAGTCATTTGCCCGGGGCACTGGTCAAAGGCCTGACCCTGACATTGTGACTGGACTTGCCGGCCCTTTCGCGGC  >  NC_002947/5639963‑5640253

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑