Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,651,374 T→G 5.1% intergenic (‑182/+31) PP_4959 ← / ← fba diguanylate cyclase/fructose‑bisphosphate aldolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,651,3740TG 5.1% 249.6 / 4.3 79intergenic (‑182/+31)PP_4959/fbadiguanylate cyclase/fructose‑bisphosphate aldolase
Reads supporting (aligned to +/- strand):  ref base T (34/40);  new base G (2/2);  total (37/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.70e-01

CGGTTGGATCCATTAGTACTCTAGACCTGAGGCTGCAACACGCAATGCAATCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCAGAGAGATCGGCTTGATCTTCGAGGCATTGCCCGCGGTGCCGAAGGCTTCGTAACGCGCGAT  >  NC_002947/5651227‑5651521
                                                                                                                                                   |                                                                                                                                                   
cGGTTGGATCCATTAGTACTCTAGACCTGAGGCTGCAACACGCAATGCAATCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCAt                                                                                                                                                     <  7:108457/148‑1 (MQ=255)
 ggTTGGATCCATTAGTACTCTAGACCTGAGGCTGCAACACGCAATGCAATCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCg                                                                                                                                                   <  3:60751/149‑1 (MQ=255)
   ttGGATCCATTAGTACTCTAGACCTGAGGCTGCAACACGCAATGCAATCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCt                                                                                                                                                 >  4:269831/1‑149 (MQ=255)
     ggATCCATTAGTACTCTAGACCTGAGGCTGCAACACGCAATGCAATCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTg                                                                                                                                                <  3:292470/148‑1 (MQ=255)
      gATCCATTAGTACTCTAGACCTGAGGCTGCAACACGCAATGCAATCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGc                                                                                                                                               <  2:345787/148‑1 (MQ=255)
      gATCCATTAGTACTCTAGACCTGAGGCTGCAACACGCAATGCAATCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCAATAAAAAAACCCGCAACgatccg                                                                                                                                              >  6:17149/1‑144 (MQ=255)
      gATCCATTAGTACTCTAGACCTGAGGCTGCAACACGCAATCCAATCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGc                                                                                                                                               <  5:353881/148‑1 (MQ=255)
        tCCATTAGTACTCTAGACCTGAGGCTGCAACACGCAATGCAATCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCgt                                                                                                                                             <  4:227811/148‑2 (MQ=255)
              aGTACTCTAGACCTGAGGCTGCAACACGCAATGCAATCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGttttt                                                                                                                                       <  5:134720/148‑1 (MQ=255)
               gTACTCTAGACCTGAGGCTGCAACACGCAATGCAATCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCa                                                                                                                                     <  1:275760/149‑1 (MQ=255)
               gTACTCTAGACCTGAGGCTGCAACACGCAATGCAATCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCa                                                                                                                                     <  2:298461/149‑1 (MQ=255)
                 aCTCTAGACCTGAGGCTTCAACACGCAATGCAATCATAACGATATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAg                                                                                                                                    <  4:39071/148‑1 (MQ=255)
                  ctctAGACCTGAGGCTGCAACACGCAATGCAATCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCAGCGATGCGGGtttttttat                                                                                                                                   <  4:278018/148‑5 (MQ=255)
                  ctctAGACCTGAGGCTGCAACACGCAATGCAATCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCAGCGATGCGGGtttttttat                                                                                                                                   <  2:77925/148‑5 (MQ=255)
                   tctAGACCTGAGGCTGCAACACGCAATGCAATCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGt                                                                                                                                 >  2:40142/1‑149 (MQ=255)
                   tctAGACCTGAGGCTGCAACACGCAATGCAATCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGt                                                                                                                                 >  6:254815/1‑149 (MQ=255)
                     tAGACCTGAGGCTGCAACACGCAATGCAATCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTa                                                                                                                               >  8:365138/1‑149 (MQ=255)
                     tAGACCTGAGGCTGCAACACGCAATGCAATCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGAACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCa                                                                                                                                     >  3:240173/1‑143 (MQ=255)
                     tAGACCTGAGGCTGCAACACGCAATGCAATCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGAACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCa                                                                                                                                     <  4:240173/143‑1 (MQ=255)
                          cTGAGGCTGCAACACGCAATGCAATCAGAACGCTATCAATGATCAAACTGTATGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGt                                                                                                                          >  2:120414/1‑149 (MQ=255)
                                     acacGCAATGCAATCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGG‑TTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGAc                                                                                                              >  8:127918/1‑149 (MQ=255)
                                      cacGCAATGCAATCAGAAAGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTTCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGAc                                                                                                              <  6:266559/149‑1 (MQ=255)
                                       acGCAATGCAATCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGAcc                                                                                                             <  4:123007/149‑1 (MQ=255)
                                         gcaatgcaatCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCTGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTc                                                                                                                         <  5:202806/135‑1 (MQ=255)
                                         gcaatgcaatCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTc                                                                                                                         >  6:202806/1‑135 (MQ=255)
                                         gcaatgcaatCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCtt                                                                                                           >  1:61324/1‑149 (MQ=255)
                                           aatgcaatCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTgg                                                                                                         >  6:305777/1‑149 (MQ=255)
                                               caatCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTggcggc                                                                                                     <  2:2501/149‑1 (MQ=255)
                                                      aaCGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCa                                                                                              >  1:26711/1‑149 (MQ=255)
                                                      aaCGCTATCAACGATCAAACTGTACGGCAGCCGACTAACGGGCGGGCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCa                                                                                              <  8:351277/149‑1 (MQ=255)
                                                         gCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGtt                                                                                                <  7:299356/144‑1 (MQ=255)
                                                         gCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGtt                                                                                                >  8:299356/1‑144 (MQ=255)
                                                             tCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCa                                                                                       <  6:31728/149‑1 (MQ=255)
                                                             tCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCa                                                                                       <  2:70765/149‑1 (MQ=255)
                                                               aaTGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCa                                                                                                   >  4:99292/1‑135 (MQ=255)
                                                               aaTGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCa                                                                                                   <  3:99292/135‑1 (MQ=255)
                                                               aaTGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGtt                                                                                                >  5:296042/1‑138 (MQ=255)
                                                               aaTGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGtt                                                                                                <  6:296042/138‑1 (MQ=255)
                                                                aTGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATc                                                                                                                     >  8:63014/1‑116 (MQ=255)
                                                                aTGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATc                                                                                                                     <  7:63014/116‑1 (MQ=255)
                                                                    tCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTggcggc                                                                                                     <  3:236861/128‑1 (MQ=255)
                                                                    tCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTggcggc                                                                                                     >  4:236861/1‑128 (MQ=255)
                                                                    tCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAgcg                                                                                >  7:295741/1‑149 (MQ=255)
                                                                      aaaCTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAgcgc                                                                               <  7:249833/148‑1 (MQ=255)
                                                                      aaaCTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAgcgc                                                                               <  2:26711/148‑1 (MQ=255)
                                                                       aaCTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTg                                                                             <  4:95520/149‑1 (MQ=255)
                                                                            tACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACa                                                                        >  4:126862/1‑149 (MQ=255)
                                                                            tACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACa                                                                        >  3:74635/1‑149 (MQ=255)
                                                                             aCGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACAt                                                                       >  6:281801/1‑149 (MQ=255)
                                                                              cGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGG‑TTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATg                                                                      <  7:127918/148‑1 (MQ=255)
                                                                                gTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACAt                                                                       <  8:99996/146‑1 (MQ=255)
                                                                                gTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACAt                                                                       >  7:99996/1‑146 (MQ=255)
                                                                                       aCTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCaga                                                             <  7:365138/149‑1 (MQ=255)
                                                                                         tAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCagaga                                                           <  3:269831/149‑1 (MQ=255)
                                                                                                 ggtcTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCAGAGAGATCGGCt                                                   <  1:40142/149‑1 (MQ=255)
                                                                                                   tcggCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGTGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCAGAGAGATCGGCTTg                                                 <  2:300657/146‑1 (MQ=255)
                                                                                                        cGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCAGAGAGATCGGCTTGATCtt                                            <  3:126862/149‑1 (MQ=255)
                                                                                                         ggtaTTATGTACATGTTTGCATCTTCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCAGAGAGATCGGCTTGATCTTc                                           <  5:17149/146‑1 (MQ=255)
                                                                                                          gAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCAGAGAGATCGGCTTGATCTTc                                           >  2:299443/1‑148 (MQ=255)
                                                                                                            aTTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCAGAGAGATCGGCTTGATCTTCGAg                                        <  8:295741/149‑1 (MQ=255)
                                                                                                             ttATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCAGAGAGATCGGCTTGATCTTCGAgg                                       >  1:374894/1‑149 (MQ=255)
                                                                                                                     aGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCAGAGAGATCGGCTTGATCTTCGAGGCATTGccc                               >  5:197451/1‑149 (MQ=255)
                                                                                                                       gTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCAGAGAGATCGGCTTGATCTTCGAGGCATTGCCcgc                             >  7:115097/1‑149 (MQ=255)
                                                                                                                       gTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCAGAGAGATCGGCTTGATCTTCGAGGCATTGCCcgc                             <  8:115097/149‑1 (MQ=255)
                                                                                                                          tcattatcCAATAAAAAAACCCGCATCGCTGCGGTTTTTTAAGGGTTACCTTTCTATCATTTTCCCTTGGCGGCCATTTCACCTATCAGGTAGCGCTGGTACATGCCTTCCAGAGAGATCGGCTTGATCTTCGAGGCATTGCCCGCgg                           <  1:120414/142‑1 (MQ=255)
                                                                                                                             aTCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCAGAGAGATCGGCTTGATCTTCGAGGCATTGCCCGCGGTGcc                       >  5:416650/1‑149 (MQ=255)
                                                                                                                                  ccATAAAAACAGCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTCGTACATGCCTTCCAGAGAGATCGGCTTGATCTTCGAGGCATTGCCCGCGGTGCCGAAg                   <  8:161794/148‑1 (MQ=255)
                                                                                                                                  ccATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACGTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCAGAGAGATCGGCTTGATCTTCGAGGCATTGCCCGCGGTGCCGAAgg                  >  8:307107/1‑149 (MQ=255)
                                                                                                                                  ccATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCa                                                               >  5:207175/1‑104 (MQ=255)
                                                                                                                                  ccATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCa                                                               <  6:207175/104‑1 (MQ=255)
                                                                                                                                  ccATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCAGAGAGATCGGCtt                                                  <  7:348526/117‑1 (MQ=255)
                                                                                                                                  ccATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCAGAGAGATCGGCtt                                                  >  8:348526/1‑117 (MQ=255)
                                                                                                                                    aTAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCAGAGAGATCGGCTTGATCTTCGAGGCATTGCCCGCGGTGCCGAAGGCt                >  4:398551/1‑149 (MQ=255)
                                                                                                                                      tgaaaaaCCCGCAGCGATGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCAGAGAGATCGGCTTGATCTTCGAGGCATTGCCCGCGGTGCCGAAGGCTTc              >  3:59128/3‑149 (MQ=255)
                                                                                                                                       aaaaaaCCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCAGAGAGATCGGCTTGATCTTCGAGGCATTGCCCGCGGTGCCGAAGGCTTCg             >  7:77188/1‑149 (MQ=255)
                                                                                                                                       aaaaaaCCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCAGAGAGATCGGCTTGATCTTCGAGGCATTGCCCGCGGTGCCGAAGGCTTCg             >  4:1817/1‑149 (MQ=255)
                                                                                                                                       aaaaaaCCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCAGAGAGATCGGCTTGATCTTCGAGGCATTGCCCGCGGTGCCGAAGGCTTCg             >  8:70920/1‑149 (MQ=255)
                                                                                                                                          aaaCCCGCAGCGATGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCAGAGAGATCGGCTTGATCTTCGAGGCATTGCCCGCGGTGCCGAAGGCTTCGTaa          >  3:306102/1‑149 (MQ=255)
                                                                                                                                             cccGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCAGAGAGATCGGCTTGATCTTCGAGGCATTGCCCGCGGTGCCGAAGGCTTCGTAAcgc       >  7:280755/1‑149 (MQ=255)
                                                                                                                                                  aTCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCAGAGAGATCGGCTTGATCTTCGAGGCATTGCCCGCGGTGCCGAAGGCTTCGTAACGCGCGAt  <  5:305777/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
CGGTTGGATCCATTAGTACTCTAGACCTGAGGCTGCAACACGCAATGCAATCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCAGAGAGATCGGCTTGATCTTCGAGGCATTGCCCGCGGTGCCGAAGGCTTCGTAACGCGCGAT  >  NC_002947/5651227‑5651521

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑