| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,655,636 | C→A | 27.2% | intergenic (‑91/+61) | epd ← / ← tktA | D‑erythrose 4‑phosphate dehydrogenase/transketolase A |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,655,636 | 0 | C | A | 27.2% | 54.8 / 19.2 | 33 | intergenic (‑91/+61) | epd/tktA | D‑erythrose 4‑phosphate dehydrogenase/transketolase A |
| Reads supporting (aligned to +/- strand): ref base C (11/13); new base A (5/4); total (16/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.08e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.02e-01 | |||||||||||
GATACGGCCATAACCGTTGAGTGCAACTTTGTAGGGACGGTGGGGCATTCGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGG‑‑‑‑CATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAATG‑AAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCGAGGATGTTCTCCAGGGTGAAGCCAAACTCTTCGAACAATGCCGAAGCCGGCGCC > NC_002947/5655498‑5655781 | gaaaCGGCCATAAGCGTTGAGGCCAACTTTGTATGGACGGTGGGGCATTCGTGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGG‑‑‑‑CATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCTACCTCtgtg < 8:175445/146‑1 (MQ=255) aTACGGCCATAACCGTTGAGTGCAACTTTGTAGGGACGGTGGGGCATTCGGGTCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGG‑‑‑‑CATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTgg < 6:201673/149‑1 (MQ=255) gCCATAACCGTTGAGTGCAACTTTGTAGGGACGGTGGGGCATTCGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTCGCCTTCATTCGCGGGCAAGCCCGCTCCCACAGAGGTTGCATGAAACC‑CTGTggg < 5:237384/149‑1 (MQ=21) ccATACCCGTGGCCTGCAACTTGGTCGGGACGGTGGGGCATTCGGGGCTCACGCGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGG‑‑‑‑CATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCgg < 4:360793/149‑1 (MQ=38) ccATAACCGTTGAGTGCAACTTTGTAGGGACGGTGGGGCATTCGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGG‑‑‑‑CATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCgg < 8:380716/149‑1 (MQ=255) cATAACCGTTGAGTGCAACTTTGTAGGGACGGTGGGGCATTCGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGG‑‑‑‑CATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCggg < 3:166342/149‑1 (MQ=255) attaCCGTTGAGTGCAACTTTGTAGGGACTGTGGGTCATTTTTGTCTCACGAGACAGTTCAATTGTAGTCAGCCTGCGGAATTTGCGGTGTGG‑‑‑‑CATTAGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCtgt < 4:301999/136‑1 (MQ=255) aTAACCGTTGAGTGCAACTTTGTAGGGACGGTGGGGCATTCGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGG‑‑‑‑CATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCtgt > 3:301999/1‑139 (MQ=255) aTAACCGTTGAGTGCAACTTTGTAGGGACGGTGGGGCATTCGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGG‑‑‑‑CATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGc < 7:56443/149‑1 (MQ=255) aCCGTTGAGTGCAACTTTGTAGGGACGGTGGGGCATTCGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGG‑‑‑‑CATTCGCGGGCAAGCCCGCTCCCACAG‑GGTATCATGCAACCTCTGTGGGAGCGGGCtt < 1:12280/148‑1 (MQ=255) cAACTTTGTAGGGACGGTGGGGCATTCGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGG‑‑‑‑CATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGccc < 1:342270/141‑1 (MQ=255) cAACTTTGTAGGGACGGTGGGGCATTCGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGG‑‑‑‑CATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGccc > 2:342270/1‑141 (MQ=255) aaCTTTGTAGGGACGGTGGGGCATTCGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGG‑‑‑‑CATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACctct < 6:46603/122‑1 (MQ=255) aaCTTTGTAGGGACGGTGGGGCATTCGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGG‑‑‑‑CATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACctct > 5:46603/1‑122 (MQ=255) gTAGGGACGGTGGGGCATTCGGGCCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGGGG‑‑‑‑CATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAATG‑AAGGCGa < 3:225679/148‑1 (MQ=255) aGGGACGGTGGGGCATTCGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGG‑‑‑‑CATTCGCGGGCAAGCCCGCTCCCACAGAGGTTGCATGAAACC‑CTGTGGGAGc > 3:361512/1‑122 (MQ=25) aGGGACGGTGGGGCATTCGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGG‑‑‑‑CATTCGCGGGCAAGCCCGCTCCCACAGAGGTTGCATGAAACC‑CTGTGGGAGc < 4:361512/122‑1 (MQ=25) gCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAATG‑AAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCGAGGATGTTCTCCAGGGTGAAGCCAAACTCTTCg > 5:54545/1‑148 (MQ=255) cccGCTCCCACAGAGGTTGCATGAAACC‑CTGTGGGAGCGGGCTTGCCCGCGAATG‑AAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCGAGGATGTTCTCCAgg > 5:216240/1‑129 (MQ=25) cccGCTCCCACAGAGGTTGCATGAAACC‑CTGTGGGAGCGGGCTTGCCCGCGAATG‑AAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCGAGGATGTTCTCCAgg < 6:216240/129‑1 (MQ=25) cccGCTCCCACAGAGGTTGCATGAAACC‑CTGTGGGAGCGGGCTTGCCCGCGAATG‑AAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCGAGGATGTTCTCCAGGGTGaa > 1:37281/1‑134 (MQ=37) cccGCTCCCACAGAGGTTGCATGAAACC‑CTGTGGGAGCGGGCTTGCCCGCGAATG‑AAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCGAGGATGTTCTCCAGGGTGaa < 2:37281/134‑1 (MQ=37) tCCCACAGAGGTTGCATGAAACC‑CTGTGGGAGCGGGCTTGCCCGCGAATG‑AAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCGAGGATGTTCTCCAGGGTGAAGCCAAACTCTTCGAACAATg > 7:177619/1‑149 (MQ=38) tCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAATG‑AAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCGAGGATGTTCTCCAGGGTGAAGCCAAACTCTTCGAACAATg > 3:181991/1‑149 (MQ=255) cacagaggTTGCATGAAACC‑CTGTGGGAGCGGGCTTGCCCGCGAATGCCACACCGCAAATTCCGCAGATCTTTAAATCAGTCTTCCAGCAGATCTTCGGCAGTACCGAGGATGTTATCCAGGGTGAAGCCAAACTCTTCGAACAATGcc > 7:197100/7‑149 (MQ=17) cacaG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAATG‑AAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCGAGGATGTTCTCCAGGGTGAAGCCAAACTCTTCGAACAATGcc > 6:370376/1‑148 (MQ=255) acaG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAATG‑AAGGCGACACCGCCAGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCGAGGATGTTCTCCAGGGTGAAGCCAAACTCTTCGAACAATGCCg > 4:314560/1‑148 (MQ=255) tttCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAATG‑AAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCGAGGATGTTCTCCAGGGTGAAGCCAAACTCTTCGAACAATGCCGAAGCCgg > 7:121760/1‑149 (MQ=255) cATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAATG‑AAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCGAGGATGTTCTCCAGGGTGAAGCCAAACTCTTCGAACAATGCCGAAGCCGgcgc > 1:241219/1‑149 (MQ=255) aTGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAATG‑AAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCg > 2:109558/1‑95 (MQ=255) aTGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAATG‑AAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCg < 1:109558/95‑1 (MQ=255) aTGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAATG‑AAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCGAGGATGTTCTCCAGGGTGAAGCCAAACTCTTCGAACAATGCCGAAGCCGgcgc < 3:314560/148‑1 (MQ=255) aTGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAATG‑AAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCGAGGATGTTCTCCAGGGTGAAGCCAAACTCTTCGAACAATGCCGAAGCCGGCGcc > 1:350659/1‑149 (MQ=255) | GATACGGCCATAACCGTTGAGTGCAACTTTGTAGGGACGGTGGGGCATTCGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGG‑‑‑‑CATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAATG‑AAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCGAGGATGTTCTCCAGGGTGAAGCCAAACTCTTCGAACAATGCCGAAGCCGGCGCC > NC_002947/5655498‑5655781 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |