Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,669,965 C→G 9.2% L148V (CTG→GTG)  metF → 5,10‑methylenetetrahydrofolate reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,669,9650CG 9.2% 258.2 / 13.0 87L148V (CTG→GTG) metF5,10‑methylenetetrahydrofolate reductase
Reads supporting (aligned to +/- strand):  ref base C (36/43);  new base G (4/4);  total (40/47)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.00e-01

GCTGGCCGAGTACAAGGCAGCCGGCATCAAACGCATTGTCGCGCTGCGTGGTGACCTGCCGTCGGGCATGGGCATGGCCAGTGGTGAACTGCGCTATGCCAGTGACCTGGTCGAGTTCATCCGCGAGGAGACCGCTGATCACTTCCACCTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGGCGCGCAACTTC‑GAGGCGGATCTGGCCAACTTTGTGCACAAGGTCAAGGCCGGTGCCGACAGCGCCATCACCCAGTACTTCTTCAACGCCGACAGCTACTTCTACTTCGTC  >  NC_002947/5669817‑5670111
                                                                                                                                                    |                                                                                                                                                   
gctgGCCGAGTACAAGGCAGCCGGCATCAAACGCATTGTCGCGCTGCGTGGTGACCTGCCGTCGGGCATGGGCATGGCCAGTGGTGAACTGCGCTATGCCAGTGACCTGGTCGAGTTCATCCGCGAGGAGACCGCTGATCACTTCCAcc                                                                                                                                                     >  1:390366/1‑149 (MQ=255)
 ctgGCCGAGTACAAGGCAGGCGGCATCAAACGGATTGTCGCGCTGCGTGGTGACCTGCCGTCGGGCATGGGCATGGCCAGTGGTGAACTGCGCTATGCCAGTGACCTGGTCGAGTTCATCCGCGAGGAGACCGCTGATCACTTCCACCt                                                                                                                                                    <  5:321254/149‑1 (MQ=255)
 ctgGCCGAGTACAAGGCAGCCGGCATCAAACGCATTGTCGCGCTGCGTGGTGACCTGTCGTCGGGCATGTGCATGGCGAGTGGTGAACTGCGCTATGCCAGTGACCTGGTCGAGTTCATCCGCGAGGAGACCGCTGATCACTTCCACCt                                                                                                                                                    <  6:367284/149‑1 (MQ=255)
    gCCGAGTACAAGGCAGCCGGCATCAAACGCATTGTCGCGCTGCGTGGTGACCTGCCGTCGGGCATGGGCATGGCCAGTGGTGAACTGCGCTATGCCAGTGACCTGGTCGAGTTCATCCGCGAGGAGACCGCTGATCACTTCCACCTgg                                                                                                                                                  >  8:280240/1‑148 (MQ=255)
      cGAGTACAAGGCAGCCGGCATCAAACGCATTGTCGCGCTGCGTGGTGACCTGCCGTCGGGCATGGGCATGGCCAGTGGTGAACTGCGCTATGCCAGTGACCTGGTCGAGTTCATCCGCGAGGAGACCGCTGATCACTTCCAGGTGGAAg                                                                                                                                               <  8:65196/149‑1 (MQ=255)
      cGAGTACAAGGCAGCCGGCATCAAACGCATTGTCGCGCTGCGTGGTGACCTGCCGTCGGGCATGGGCATGGCCAGTGGTGAACTGCGCTATGCCAGTGACCTGGTCGAGTTCATCCGCGAGGAGACCGCTGATCACTTCCACCTGGAAg                                                                                                                                               >  5:217194/1‑149 (MQ=255)
       gAGTACAAGGCAGCCGGCATCAAACGCATTGTCGCGCTGCGTGGTGACCTGCCGTCGGGCATGGGCATGGCCAGTGGTGAACTGCGCTATGCCAGTGACCTGGTCGAGTTCATCCGCGAGGAGACCGCTGATCACTTCCACCTGGAAGt                                                                                                                                              >  7:117212/1‑149 (MQ=255)
       gAGTACAAGGCAGCCGGCATCAAACGCATTGTCGCGCTGCGTGGTGACCTGCCGTCGGGCATGGGCATGGCCAGTGGTGAACTGCGCTATGCCAGTGACCTGGTCGAGTTCATCCGCGAGGAGACCGCTGATCACTTCCACCTGGAAGt                                                                                                                                              <  1:313101/149‑1 (MQ=255)
         gTACAAGGCAGCCTGCATCAAACGCATTGTCGCGCTGCGTGGTGACCTGCCGTCGGGCATGGGCATGGCCAGTGGTGAACTGCGCTATGCCAGTGACCTGGTCGAGTTCATCCGCGAGGAGACCGCTGATCACTTCCACCTGGAAGTgg                                                                                                                                            <  3:302417/149‑1 (MQ=255)
          tACAAGGCAGCCGGCATCAAACGCATTGTCGCGCTGCGTGGTGACCTGCCGTCGGGCATGGGCATGGCCAGTGGTGAACTGCGCTATGCCAGTGACCTGGTCGAGTTCATCCGCGAGGAGACCGCTGATCACTTCCACCTGGAAGTGGc                                                                                                                                           >  5:222595/1‑149 (MQ=255)
          tACAAGGCAGCCGGCATCAAACGCATTGTCGCGCTGCGTGGTGACCTGCCGTCGGGCATGGGCATGGCCAGTGGTGAACTGCGCTATGCCAGTGACCTGGTCGAGTTCATCCGCGAGGAGACCGCTGATCACTTCCACCTGGAAGTGGc                                                                                                                                           >  6:305288/1‑149 (MQ=255)
            cAAGGCAGCCGGCATCAAACGCATTGTCGCGCTGCGTGGTGACCTGCCGTCGGGCATGGGCATGGCCAGTGGTGAACTGCGCTATGCCAGTGACCTGGTCGAGTTCATCCGCGAGGAGACCGCTGATCACTTCCACCTGGAAGTGGCt                                                                                                                                          <  1:40285/148‑1 (MQ=255)
             aaGGCAGCCGGCATCAAACGCATTGTCGCGCTGCGTGGTGACCTGCCGTCGGGCATGGGCATGGCCAGTGGTGAACTGCGCTATGCCAGTGACCTGGTCGAGTTCATCCGCGAGGAGACCGCTGATCACTTCCAGGTGGAAGTgatcag                                                                                                                                        <  4:318783/149‑6 (MQ=255)
             aaGGCAGCCGGCATCAAACGCATTGTCGCGCTGCGTGGTGACCTGCCGTCGGGCATGGGCATGGCCAGTGGTGAACTGCGCTATGCCAGTGACCTGGTCGAGTTCATCCGCGAGGAGACCGCTGATCACTTCCACCTGGAAGTGGCTGc                                                                                                                                        >  6:244216/1‑149 (MQ=255)
               ggCAGCCGGCATCAAACGCATTGTCGCGCTGCGTGGTGACCTGCCGTCGGGCATGGGCATGGCCAGTGGTGAACTGCGCTATGCCAGTGACCTGGTCGAGTTCATCCGCGAGGAGACCGCTGATCACTTCCAGGTGGAAGTgatcagcg                                                                                                                                      <  6:306072/149‑8 (MQ=255)
                gCAGCCGGCATCAAACGCATTGTCGCGCTGCGTGGTGACCTGCCGTCGGGCATGGGCATGGCCAGTGGTGAACTGCGCTATGCCAGTGACCTGGTCGAGTTCATCCGCGAGGAGACCGCTGATCACTTCCACCTGGAAGTGGCTGCCTa                                                                                                                                     >  5:176300/1‑149 (MQ=255)
                  aGCCGGCATCAAACGCATTGTCGCGCTGCGTGGTGACCTGCCGTCGGGCATGGGCATGGCCAGTGGTGAACTGCGCTATGCCAGTGACCTGGTCGAGTTCATCCGCGAGGAGACCGCTGATCACTTCCACCTGGAAGTGGCTGCCTAcc                                                                                                                                   <  8:49370/149‑1 (MQ=255)
                  aGCCGGCATCAAACGCATTGTCGCGCTGCGTGGTGACCTGCCGTCGGGCATGGGCATGGCCAGTGGTGAACTGCGCTATGCCAGTGACCTGGTCGAGTTCATCCGCGAGGAGACCGCTGATCACTTCCACCTGGAAGTGGCTGCCTAcc                                                                                                                                   >  8:63681/1‑149 (MQ=255)
                         aTCAAACGCATTGTCGCGCTGCGTGGTGACCTGCCGTCGGGCATGGGCATGGCCAGTGGTGAACTGCGCTATGCCAGTGACCTGGTCGAGTTCATCCGCGAGGAGACCGCTGATCACTTCCACCTGGAAGTGGCTGCCTACCCGGAAAt                                                                                                                            <  4:220688/149‑1 (MQ=255)
                         aTCAAACGCATTGTCGCGCTGCGTGGTGACCTGCCGTCGGGCATGGGCATGGCCAGTGGTGAACTGCGCTATGCCAGTGACCTGGTCGAGTTCATCCGCGAGGAGACCGCTGATCACTTCCACCTGGAAGTGGCTGCCTACCCGGAAAt                                                                                                                            >  5:244843/1‑149 (MQ=255)
                             aaCGCATTGTCGCGCTGCGTGGTGACCTGCCGTCGGGCATGGGCATGGCCAGTGGTGAACTGCGCTATGCCAGTGACCTGGTCGAGTTCATCCGCGAGGAGACCGCTGATCACTTCCACCTGGAAGTGGCTGCCTACCCGGAAATGCAc                                                                                                                        <  8:328345/149‑1 (MQ=255)
                              aCGCATTGTCGCGCTGCGTGGTGACCTGCCGTCGGGCATGGGCATGGCCAGTGGTGAACTGCGCTATGCCAGTGACCTGGTCGAGTTCATCCGCGAGGAGACCGCTGATCACTTCCACCTGCAAGTGGCTGCCTACCCGGAAATGCAcc                                                                                                                       >  3:252141/1‑149 (MQ=255)
                               cGCATTGTCGCGCTGCGTGGTGACCTGCCGTCGGGCATGGGCATGGCCAGTGGTGAACTGCGCTATGCCAGTGACCTGGTCGAGTTCATCCGCGAGGAGACCGCTGATCACTTCCACCTGGAAGTGGCTGCCTACCCGGAAATGCAccc                                                                                                                      <  4:83445/149‑1 (MQ=255)
                               cGCATTGTCGCGCTGCGTGGTGACCTGCCGTCGGGCATGGGCATGGCCAGTGGTGAACTGCGCTATGCCAGTGACCTGGTCGAGTTCATCCGCGAGGAGACCGCTGATCACTTCCACCTGGAAGTGGCTGCCTACCCGGAAATGCAccc                                                                                                                      <  1:78580/149‑1 (MQ=255)
                                      tCGCGCTGCGTGGTGACCTGCCGTCGGGCATGGGCATGGCCAGTGGTGAACTGCGCTATGCCAGTGACCTGGTCGAGTTCATCCGCGAGGAGACCGCTGATCACTTCCACCTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGgcg                                                                                                               >  7:152260/1‑149 (MQ=255)
                                         cgcTGCGTGGTGACCTGCCGTCGGGCATGGGCATGGCCAGTGGTGAACTGCGCTATGCCAGTGACCTGGTCGAGTTCATCCGCGAGGAGACCGCTGATCACTTCCACCTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGgcgcgc                                                                                                            <  8:152260/149‑1 (MQ=255)
                                         cgcTGCGTGGTGACCTGCCGTCGGGCATGGGCATGGCCAGTGGTGAACTGCGCTATGCCAGTGACCTGGTCGAGTTCATCCGCGAGGAGACCGCTGATCACTTCCACCTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGgcgcgc                                                                                                            <  6:244843/149‑1 (MQ=255)
                                          gcTGCGTGGTGACCTGCCGTCGGGCATGGGAATGGCCAGTGGTGAACTGCGCTATGCCAGTGACCTGGTCGAGTTAATCCGCGAGGAGACCGCTGATCACTTACACCTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGgcgcgc                                                                                                            >  7:255068/1‑148 (MQ=255)
                                              cGTGGTGACCTGCCGTCGGGCATGGGCATGGCCAGTGGTGAACTGCGCTATGCCAGTGACCTGGTCGAGTTCATCCGCGAGGAGACCGCTGATCACTTCCACCTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGGCGCGCAACtt                                                                                                       <  4:252141/149‑1 (MQ=255)
                                                 ggtgACCTGCCGTCGGGCATGGGCATGGCCAGTGGTGAACTGCGCTATGCCAGTGACCTGGTCGAGTTCATCCGCGAGGAGACCGCTGATCACTTCCACCTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGGCGCGCAACTTc                                                                                                      >  1:112494/1‑147 (MQ=255)
                                                  gtgACCTGCCGTCGGGCATGGGCATGGCCAGTGGTGAACTGCGCTATGCCAGTGACCTGGTCGAGTTCATCCGCGAGGAGACCGCTGATCACTTCCACCTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGGCGCGCAACTTC‑GAg                                                                                                  <  7:63681/149‑1 (MQ=255)
                                                   tgACCTGCCGTCGGGCTTGGGCATGGCCAGTGGTGAACTGCGCTATGCCAGTGACCTGGTCGAGTTCATCCGCGAGGAGACCGCTGATCACTTCCACCTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGGCGCGCAACTTC‑GAgg                                                                                                 >  7:159074/1‑149 (MQ=255)
                                                     aCCTGCCGTCGGGCATGGGCATGGCCAGTGGTGAACTGCGCTATGCCAGTGACCTGGTCGAGTTCATCCGCGAGGAGACCGCTGATCACTTCCACCTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGGCGCGCAACTTC‑GAGGCg                                                                                               <  3:136726/149‑1 (MQ=255)
                                                     aCCTGCCGTCGGGCATGGGCATGGCCAGTGGTGAACTGCGCTATGCCAGTGACCTGGTCGAGTTCATCCGCGAGGAGACCGCTGATCACTTCCACCTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGGCGCGCAACTTC‑GAGGCg                                                                                               <  6:222595/149‑1 (MQ=255)
                                                     aCCTGCCGTCGGGCATGGGCATGGCCAGTGGTGAACTGCGCTATGCCAGTGACCTGGTCGAGTTCATCCGCGAGGAGACCGCTGATCACTTCCACCTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGGCGCGCAACTTC‑GAGGCg                                                                                               >  1:259533/1‑149 (MQ=255)
                                                      ccTGCCGTCGGGCATGGGCATGGCCAGTGGTGAACTGCGCTATGCCAGTGACCTGGTCGAGTTCATCCGCGAGGAGACCGCTGATCACTTCCACCTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGGCGCGCAACTTC‑GAGGCgg                                                                                              <  3:9552/149‑1 (MQ=255)
                                                         gCCGTCGGGCATGGGCATGGCCAGTGGTGAACTGCGCTATGCCAGTGACCTGGTCGAGTTCATCCGCGAGGAGACCGCTGATCACTTCCACCTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGGCGCGCAACTTC‑GAGGCGGATc                                                                                           >  6:256728/1‑149 (MQ=255)
                                                          ccGTCGGGCATGGGCATGGCCAGTGGTGAACTGCGCTATGCCAGTGACCTGGTCGAGTTCATCCGCGAGGAGACCGCTGATCACTTCCACCTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGGCGCGCAACTTC‑GAGGCGGAt                                                                                            <  1:101426/147‑1 (MQ=255)
                                                          ccGTCGGGCATGGGCATGGCCAGTGGTGAACTGCGCTATGCCAGTGACCTGGTCGAGTTCATCCGCGAGGAGACCGCTGATCACTTCCACCTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGGCGCGCAACTTC‑GAGGCGGATCt                                                                                          <  8:117212/149‑1 (MQ=255)
                                                          ccGTCGGGCATGGGCATGGCCAGTGGTGAACTGCGCTATGCCAGTGACCTGGTCGAGTTCATCCGCGAGGAGACCGCTGATCACTTCCACCTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGGCGCGCAACTTC‑GAGGCGGATCt                                                                                          >  6:348896/1‑149 (MQ=255)
                                                            gTCGGGCATGGGCATGGCCAGTGGTGAACTGCGCTATGCCAGTGACCTGGTCGAGTTCATCCGCGAGGAGACCGCTGATCACTTCCACCTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGGCGCGCAACTTC‑GAGGCGGATCTgg                                                                                        >  3:343661/1‑149 (MQ=255)
                                                             tCGGGCATGGGCATGGCCAGTGGTGAACTGCGCTATGCCAGTGACCTGGTCGAGTTCATCCGCGAGGAGACCGCTGATCACTTCCACCTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGGCGCGCAACTTC‑GAGGCGGATCTGGc                                                                                       >  4:229998/1‑149 (MQ=255)
                                                                 gcatgggcatggCCAGTGGTGAACTGCGCTATGCCAGTGACCTGGTCGAGTTCATCCGCGAGGAGACCGCTGATCACTTCCACCTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGGCGCGCAACTTC‑GAGGCGGATCTGGCCAAc                                                                                   <  5:244216/149‑1 (MQ=255)
                                                                  catgggcatgtCCAGTGGTGAACTGCGCTATGCCAGTGACCTGGTCGAGTTCATCCGCGAGGAGACCGCTGATCACTTCCACCTGGAAGTGGCTGCCTAGCCTGAAATTCACCCGCATGCGAGCAACTTC‑GAGGCGGATATgttcaagt                                                                                  <  6:285367/149‑8 (MQ=255)
                                                                      ggcatggCCAGTGGTGAACTGCGCTATGCCAGTGACCTGGTCGAGTTCATCCGCGAGGAGACCGCTGATCACTTCCACCTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGGCGCGCAACTTC‑GAGGCGGATCTGGCCAACTTtgt                                                                              <  3:229998/149‑1 (MQ=255)
                                                                      ggcatggCCAGTGGTGAACTGCGCTATGCCAGTGACCTGGTCGAGTTCATCCGCGAGGAGACCGCTGATCACTTCCACCTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGGCGCGCAACTTC‑GAGGCGGATCTGGCCAACTTtg                                                                               <  3:283503/148‑1 (MQ=255)
                                                                       gcatggCCAGTGGTGAACTGCGCTATGCCAGTGACCTGGTCGAGTTCATCCGCGAGGAGACCGCTGATCACTTCCACCTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGGCGCGCAACTTC‑GAGGCg                                                                                               >  7:81061/1‑131 (MQ=255)
                                                                       gcatggCCAGTGGTGAACTGCGCTATGCCAGTGACCTGGTCGAGTTCATCCGCGAGGAGACCGCTGATCACTTCCACCTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGGCGCGCAACTTC‑GAGGCg                                                                                               <  8:81061/131‑1 (MQ=255)
                                                                          tggCCAGTGGTGAACTGCGCTATGCCAGTGACCTGGTCGAGTTCATCCGCGAGGAGACCGCTGATCACTTCCACCTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGGCGCGCAACTTC‑GAGGCGGATCTGGCCAACTTTGTGcac                                                                          <  6:206640/149‑1 (MQ=255)
                                                                                 tggtgAACTGCGCTATGCCAGTGACCTGGTCGAGTTCATCCGCGAGGAGACCGCTGATCACTTCCACCTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGGCGCGCAACTTC‑GAGGCGGATCTGGCCAACTTTGTGCACAAGGTCa                                                                   <  8:159074/149‑1 (MQ=255)
                                                                                      aaCTGCGCTATGCCAGTGACCTGGTCGAGTTCATCCGCGAGGAGACCGCTGATCACTTCCACCTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGGCGCGCAACTTC‑GAGGCGGATCTGGCCAACTTTGTGCACAAGGTCAAGGcc                                                              <  2:259533/149‑1 (MQ=255)
                                                                                         tGCGCTATGCCAGTGACCTGGTCGAGTTCATCCGCGAGGAGACCGCTGATCACTTCCACCTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGGCGCGCAACTTC‑GAGGCGGATCTGGCCAACTTTGTGCACAAGGTCAAGGCCGGt                                                           >  4:214486/1‑149 (MQ=255)
                                                                                           cgcTATGCCAGTGACCTGTTCGAGGTCATCCGCGAGGAGACCGCTGATCACTTCCACCTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGGCGCGCAACTTC‑GAGGCGGATCTGGCCAACTTTGTGCACAAGGTCAAGGCCGGt                                                           <  4:82310/147‑1 (MQ=255)
                                                                                            gcTATGCCAGTGACCTGGTCGATTTCATCCGCGATGAGACCGCTGATCACTTCCACCTGGAAGTGGCTTCCTACCCGGAAATGCACCCGCAGGCGCGCAACTTC‑GAGGCGGATCTGGCCAACTTTGTGCACAAGGTCAAGGCCGGTGcc                                                        <  8:255068/149‑1 (MQ=255)
                                                                                            gcTATGCCAGTGACCTGGTCGAGTTCATCCGCGAGGAGACCGCTGATCACTTCCAGCTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGGCGCGCAACTTC‑GAGGCGGATCTGGCCAACTTTGTGCACAAGGTCAAGGCCGGTGcc                                                        <  2:390366/149‑1 (MQ=255)
                                                                                                  ccAGTGACCTGGTCGAGTTCATCCGCGAGGAGACCGCTGATCACTTCCACCTGGAAGTGGCTGCCTACCCGGTAATGCACCCGCAGGCGCGCAACTTC‑GAGGCGGATCTGGCCAACTTTGTGCACAAGGTCAAGGCCGGTGCCGACAg                                                   <  5:305288/148‑1 (MQ=255)
                                                                                                     gTGACCTGGTCGAGTTCATCCGCGAGGAGACCGCTGATCACTTCCACCTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGGCGCGCAACTTC‑GAGGCGGATCTGGCCAACTTTGTGCACAAGGTCAAGGCCGGTGCCGACAGCGcc                                               <  7:280240/149‑1 (MQ=255)
                                                                                                        aCCTGGTCGAGTTCATCCGCGAGGAGACCGCTGATCACTTCCACCTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGGCGCGCAACTTC‑GAGGCGGATCTGGCCAACTTTGTGCACAAGGTCAAGGCCGGTGCCGACAGCGCCAt                                             >  5:306525/1‑148 (MQ=255)
                                                                                                           tGGTCGAGTTCATCCGCGAGGAGACCGCTGATCACTTCCACCTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGGCGCGCAACTTC‑GAGGCGGATCTGGCCAACTTTGTGCACAAGGTCAAGGCCGGTGCCGACAGCGCCATCAcc                                         >  3:214832/1‑149 (MQ=255)
                                                                                                             gTCGAGTTCATCCGCGAGGAGACCGCTGATCACTTCCACCTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGGCGCGCAACTTC‑GAGGCGGATCTGGCCAACTTTGTGCACAAGGTCAAGGCCGGTGCCGACAGCGCCATCAcc                                         >  4:191617/1‑147 (MQ=255)
                                                                                                                gAGTTCATCCGCGAGGAGACCGCTGATCACTTCCACCTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGGCGCGCAACTTC‑GAGGCGGATCTGGCCAACTTTGTGCACAAGGTCAAGGCCGGTGCCGACAGCGCCATCACCCAGTa                                    >  2:179688/1‑149 (MQ=255)
                                                                                                                  gTTCATCCGCGAGGAGACCGCTGATCACTTCCACCTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGGCGCGCAACTTC‑GAGGCGGATCTGGCCAACTTTGTGCACAAGGTCAAGGCCGGTGCCGACAGCGCCATCACCCAGTAct                                  >  2:207569/1‑149 (MQ=255)
                                                                                                                  gTTCATCCGCGAGGAGACCGCTGATCACTTCCACCTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGGCGCGCAACTTC‑GAGGCGGATCTGGCCAACTTTGTGCACAAGGTCAAGGCCGGTGCCGACAGCGCCATCACCCAGTAct                                  >  4:163554/1‑149 (MQ=255)
                                                                                                                   ttCATCCGCGAGGAGACCGCTGATCACTTCCACCTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGGCGCGCAACTTC‑GAGGCGGATCTGGCCAACTTTGTGCACAAGGTCAAGGCCGGTGCCGACAGCGCCATCACCCAGTActt                                 >  1:22725/1‑149 (MQ=255)
                                                                                                                      aTCCGCGAGGAGACCGCTGATCACTTCCACCTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGGCGCGCAACTTC‑GAGGCGGATCTGGCCAACTTTGTGCACAAGGTCAAGGCCGGTGCCGACAGCGCCATc                                            <  6:375824/135‑1 (MQ=255)
                                                                                                                      aTCCGCGAGGAGACCGCTGATCACTTCCACCTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGGCGCGCAACTTC‑GAGGCGGATCTGGCCAACTTTGTGCACAAGGTCAAGGCCGGTGCCGACAGCGCCATc                                            >  5:375824/1‑135 (MQ=255)
                                                                                                                       tCCGCGAGGAGACCGCTGATCACTTCCACCTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGGCGCGCAACTTC‑GAGGCGGATCTGGCCAACTTTGTGCACAAGGTCAAGGCCGGTGCCGACAGCGCCATCACCCAGTACttcttc                             <  6:217194/149‑1 (MQ=255)
                                                                                                                         cgcgAGGAGACCGCTGATCACTTCCACCTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGGCGCGCAACTTC‑GAGGCGGATCTGGCCAACTTTGTGCACAAGGTCAAGGCCGGTGCCGACAGCGCCATCACCCAGTACTTCTTCaa                           <  4:343661/149‑1 (MQ=255)
                                                                                                                         cgcgAGGAGACCGCTGATCACTTCCACCTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGGCGCGCAACTTC‑GAGGCGGATCTGGCCAACTTTGTGCACAAGGTCAAGGCCGGTGCCGACAGCGCCATCACCCAGTACTTCTTCa                            <  5:348896/148‑1 (MQ=255)
                                                                                                                          gcgAGGAGACCGCTGATCACTTCCACCTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGGCGCGCAACTTC‑GAGGCGGATCTGGCCAACTTTGTGCACAAGGTCAAGGCCGGTGCCGACAGCGCCATCACCCAGTACTTCTTCAAc                          >  8:159519/1‑149 (MQ=255)
                                                                                                                            gaggagACCGCTGATCACTTCCACCTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGGCGCGCAACTTC‑GAGGCGGATCTGGCCAACTTTGTGCACAAGGTCAAGGCCGGTGCCGACAGCGCCATCACCCAGTACTTCTTCAACGc                        <  3:191617/149‑1 (MQ=255)
                                                                                                                                    cGCTGATCACTTCCACCTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGGCGCGCAACTTC‑GAGGCGGATCTGGCCAACTTTGTGCACAAGGTCAAGGCCGGTGCCGACAGCGCCATCACCCAGTACTTCTTCAACGCCGACAGc                 >  6:356623/1‑148 (MQ=255)
                                                                                                                                      cTGATCACTTCCACCTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGGCGCGCAACTTC‑GAGGCGGATCTGGCCAACTTTGTGCACAAGGTCAAGGCCGGTGCCGACAGCGCCATCACCCAGTACTTCTTCAACGCCGACAGctac              >  2:392782/1‑149 (MQ=255)
                                                                                                                                        agccACTTCCAGGTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGGCGCGCAACTTC‑GAGGCGGATCTGGCCAACTTTGTGCACAAGGTCAAGGCCGGTGCCGACAGCGCCATCACCCAGTACTTCTTCAACGCCGACAGctactt            >  7:317462/4‑149 (MQ=255)
                                                                                                                                          tCACTTCCACCTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGGCGCGCAACTTC‑GAGGCGGATCTGGCCAACTTTGTGCACAAGGTCAAGGCCGGTGCCGACAGCGCCATCACCCAGTACTTCTTCAACGCCGACAGctacttc           >  3:65266/1‑148 (MQ=255)
                                                                                                                                          ccACTTCCAGGTGGAAGTGGCTGCCTACCCGGAAATGGACCCTCAGGCGCGCAACTTC‑GAGGCGGATCTGGCCAACTTTGTGCACAAGGTCTAGGCCGGTGCCGACAGCGCCATCACCCAGTActtct                               <  3:335107/127‑1 (MQ=255)
                                                                                                                                          ccACTTCCAGGTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGGCGCGCAACTTCGGGGGCGGATCTGGCCAACTTTGTGCACAAGGTCAAGGCCGGTGCCGACAGCGCCATCACCCAGTACTTCTTCAACGCCGACAGctacttc           >  1:115409/2‑149 (MQ=255)
                                                                                                                                          ccACTTCCAGGTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGGCGCGCAACTTC‑GAGGCGGATCTGGCCAACTTTGTGCACAAGGTCAAGGCCGGTGCCGACAGCGCCATCACCCAGTACTTCTTCAACGCCGACAGctacttc           >  8:104406/2‑148 (MQ=255)
                                                                                                                                          ccACTTCCACGTGGAAGTGGCTGCCTACCCGGAAATGCACCCCCAGGCGCGCAACTTC‑GAGGCGGATCTGGCCAACTTAGTGCACAAGGTCAAGGCCGGTGCCGACAGCGCCATCACCCACTActtct                               >  4:335107/2‑128 (MQ=255)
                                                                                                                                            aCTTCCACCTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGGCGCGCAACTTC‑GAGGCGGATCTGGCCAACTTTGTGCACAAGGTCAAGGCCGGTGCCGACAGCGCCATCACCCCGTACTTCTTCAACGCCGACAGctacttctac        <  3:341154/149‑1 (MQ=255)
                                                                                                                                            aCTTCCACCTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGGCGCGCAACTTC‑GAGGCGGATCTGGCCAACTTTGTGCACAAGGTCAAGGCCGGTGCCGACAGCGCCATCACCCAGTACTTCTTCAACGCCGACAGctacttctac        <  1:392782/149‑1 (MQ=255)
                                                                                                                                            aCTTCCACCTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGGCGCGCAACTTC‑GAGGCGGATCTGGCCAACTTTGTGCACAAGGTCAAGGCCGGTGCCGACAGCGCCATCACCCAGTACTTCTTCAACGCCGACAGctacttcta         <  3:214486/148‑1 (MQ=255)
                                                                                                                                                ccACCTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGGCGCGCAACTTC‑GAGGCGGATCTGGCCAACTTTGTGCACAAGGTCAAGGCCGGTGCCGACAGCGCCATCACCCAGTACTTCTTCAACGCCGACAGCtacttctacttc     <  6:23939/148‑1 (MQ=255)
                                                                                                                                                ccACCTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGGCGCGCAACTTC‑GAGGCGGATCTGGCCAACTTTGTGCACAAGGTCAAGGCCGGTGCCGACAGCGCCATCACCCAGTACTTCTTCAACGCCGACAGCtacttctacttc     >  5:23939/1‑148 (MQ=255)
                                                                                                                                                ccACCTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGGCGCGCAACTTC‑GAGGCGGATCTGGCCAACTTTGTGCACAAGGTCAAGGCCGGTGCCGACAGCGCCATCACCCAGTACTTCTTCAACGCCGACAGCTACTTCTACTtcg    >  2:121465/1‑149 (MQ=255)
                                                                                                                                                  aCCTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGGCGCGCAACTTC‑GAGGCGGATCTGGCCAACTTTGTGCACAAGGTCAAGGCCGGTGCCGACAGCGCCATCACCCAGTACTTCTTCAACGCCGACAGCTACTTCTACTtcgtc  >  8:129336/1‑149 (MQ=255)
                                                                                                                                                  aCCTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGGCGCGCAACTTC‑GAGGCGGATCTGGCCAACTTTGTGCACAAGGTCAAGGCCGGTGCCGACAGCGCCATCACCCAGTACTTCTTCAACGCCGACAGCTACTTCTACTtcgtc  <  4:214832/149‑1 (MQ=255)
                                                                                                                                                  aCCTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGGCGCGCAACTTC‑GAGGCGGATCTGGCCAACTTTGTGCACAAGGTCAAGGCCGGTGCCGACAGCGCCATCACCCAGTACTTCTTCAACGCCGACAGCTACTTCTACTtcgtc  <  6:306525/149‑1 (MQ=255)
                                                                                                                                                    cTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGGCGCGCAACTTC‑GAGGCGGATCTGGCCAACTTTGTGCACAAGGTCAAGGCCGGTGCCGACAGCGCCATCAcc                                         <  5:374488/108‑1 (MQ=255)
                                                                                                                                                    cTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGGCGCGCAACTTC‑GAGGCGGATCTGGCCAACTTTGTGCACAAGGTCAAGGCCGGTGCCGACAGCGCCATCAcc                                         >  6:374488/1‑108 (MQ=255)
                                                                                                                                                    cTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGGCGCGCAACTTC‑GAGGCGGATCTGGCCAACTTTGTGCACAAGGTCAAGGCCGGTGCCGACAGCGCCATCACCCAGTACTTCTTCAACGCCGACAGCTACTTCTACTtcgtc  >  2:237621/1‑147 (MQ=255)
                                                                                                                                                    cTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGGCGCGCAACTTC‑GAGGCGGATCTGGCCAACTTTGTGCACAAGGTCAAGGCCGGTGCCGACAGCGCCATCACCCAGTACTTCTTCAACGCCGACAGCTACTTCTACTtcgtc  <  1:237621/147‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GCTGGCCGAGTACAAGGCAGCCGGCATCAAACGCATTGTCGCGCTGCGTGGTGACCTGCCGTCGGGCATGGGCATGGCCAGTGGTGAACTGCGCTATGCCAGTGACCTGGTCGAGTTCATCCGCGAGGAGACCGCTGATCACTTCCACCTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGGCGCGCAACTTC‑GAGGCGGATCTGGCCAACTTTGTGCACAAGGTCAAGGCCGGTGCCGACAGCGCCATCACCCAGTACTTCTTCAACGCCGACAGCTACTTCTACTTCGTC  >  NC_002947/5669817‑5670111

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑