| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,689,871 | T→C | 5.6% | D8G (GAC→GGC) | pilH ← | twitching motility protein PilH |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,689,871 | 0 | T | C | 5.6% | 270.0 / 4.1 | 92 | D8G (GAC→GGC) | pilH | twitching motility protein PilH |
| Reads supporting (aligned to +/- strand): ref base T (47/38); new base C (2/3); total (50/42) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.56e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.38e-01 | |||||||||||
ATGACGATGTCCATCAGCACCGCGTCGGGCTTGTCCTGCCGGGCCAGGGCCACGCCGTCGGCACCGTTGCTGGCCTTGAGCACCTGGTAGCCGTG‑CTTTTCCAGCCATTCGGTCAATCTGTACATCTCTGTCGGCGAGTCGTCGACAATCAGAACTCGGGCCATGCTGTTTCCCCAT‑CAGGAAAGTAGGACCGCGCCATGGCGGGGCTCGGTCAGGGTGCGTGTTGTTGAGGTGCGGCAAACCCGGGCACATGGGCGCGGATCGCGTCGAGCAGTTCTTCCTTGCTGAAC > NC_002947/5689730‑5690019 | aTGACGATGTCCATCAGCACCGCGTCGGGCTTGTCCTGCCGGGCCAGGGCCACGCCGTCGGCACCGTTGCTGGCCTTGAGCACCTGGTAGCCGTG‑CTTTTCCAGCCATTCGGTCAATCTGTACATCTCTGTCGGCGAGTCGTCGACaa > 1:301329/1‑148 (MQ=255) tGACGATGTCCATCAGCACCGCGTCGGGCTTGTCCTGCCGGGCCAGGGCCACGCCGTCGGCACCGTTGCTGGCCTTGAGCACCTGGTAGCCGTG‑CTTTTCCAGCCATTCGGTCAATCTGTACATCTCTGTCGGCGAGTCGTCGACAATc < 3:225163/149‑1 (MQ=255) tGACGATGTCCATCAGCACCGCGTCGGGCTTGTCCTGCCGGGCCAGGGCCACGCCGTCGGCACCGTTGCTGGCCTTGAGCACCTGGTAGCCGTG‑CTTTTCCAGCCATTCGGTCAATCTGTACATCTCTGTCGGCGAGTCGTCGACAATc < 4:6594/149‑1 (MQ=255) gACGATGTCCATCAGCACCGCGTCGGGCTTGTCCTGCCGGGCCAGGGCCACGCCGTCGGCACCGTTGCTGGCCTTGAGCACCTGGTAGCCGTG‑CTTTTCCAGCCATTCGGTCAATCTGTACATCTCTGTCGGCGAGTCGTCGACAAt > 4:272499/1‑147 (MQ=255) aCGATGTCCATCTGCACCGCGTCGGGCTTGTCCTGCCGGGCGAGGGCCACGCCGTCGGCACCGTTGCTGGCCTTGAGCACCTGGTAGCCGTG‑CTTTTCCAGCCATTCGGTCAATGTGTACATCTCTGTCGACGACTCGCCGagagagat < 4:274297/149‑8 (MQ=255) gTCCATCAGCACCGCGTCGGGCTTGTCCTGCCGGGCCAGGGCCACGCCGTCGGCACCGTTGCTGGCCTTGAGCACCTGGTAGCCGTG‑CTTTTCCAGCCATTCGGTCAATCTGTACATCTCTGTCGGCGAGTCGTCGACAATCAGAACTc > 5:395118/1‑149 (MQ=255) tCCATCAGCACCGCGTCGGGCTTGTCCTGCCGGGCCAGGGCCACGCCGTCGGCACCGTTGCTGGCCTTGAGCACCTGGTAGCCGTG‑CTTTTCCAGCCATTCGGTCAATCTGTACATCTCTGTCGGCGAGTCGTCGACAATCAGAACTc < 3:96236/148‑1 (MQ=255) ccATCAGCACCGCGTCGGGCTTGTCCTGCCGGGCCAGGGCCACGCCGTCGGCACCGTTGCTGGCCTTGAGCACCTGGTAGCCGTG‑CTTTTCCAGCCATTCGGTCAATCTGTACATCTCTGTCGACGACTCGCCGACagag < 8:62978/140‑4 (MQ=255) ccATCAGCACCGCGTCGGGCTTGTCCTGCCGGGCCAGGGCCACGCCGTCGGCACCGTTGCTGGCCTTGAGCACCTGGTAGCCGTG‑CTTTTCCAGCCATTCGGTCAATCTGTACATCTCTGTCGACGACTCGCCGACagag > 7:62978/1‑137 (MQ=255) cATCAGCACCGCGTCGGGCTTGTCCTGCCGGGCCAGGGCCACGCCGTCGGCACCGTTGCTGGCCTTGAGCACCTGGTAGCCGTG‑CTTTTCCAGCCATTCGGTCAATCTGTACATCTCTGTTGGCGAGTCGTCGACAATCAGAACTCggg > 6:245998/1‑149 (MQ=255) cATCAGCACCGCGTCGGGCTTGTCCTGCCGGGCCAGGGCCACGCCGTCGGCACCGTTGCTGGCCTTGAGCACCTGGTAGCCGTG‑CTTTTCCAGCCATTCGGTCAATCTGTACATCTCTGTCGGCGAGTCGTCGACAATCAGAACTCggg > 5:18623/1‑149 (MQ=255) aTCAGCACCGCGTCGGGCTTGTCCTGCCGGGCCAGGGCCACGCCGTCGGCACCGTTGCTGGCCTTGAGCACCTGGTAGCCGTG‑CTTTTCCAGCCATTCGGTCAATCTGTACATCTCTGTCGGCGAGTCGTCGACAATCAGAACTCGGGc > 3:75199/1‑149 (MQ=255) cACCGCGTCGGGCTTGTCCTGCCGGGCCAGGGCCACGCCGTCGGCACCGTTGCTGGCCTTGAGCACCTGGTAGCCGTG‑CTTTTCCAGCCATTCGGTCAATCTGTACATCTCTGTCGGCGAGTCGTCGACAATCAGAACTCGGGCCATGc > 4:298043/1‑149 (MQ=255) aCCGCGTCGGGCTTGTCCTGCCGGGCAAGGGCCACGCCGTCGGCACCGTTGCTGGCCTTGAGCACCTGGTAGCCGTG‑CTTTTCCAGCCATTCGGTCAATCTGTACATCTCTGTCGGCGAGTCGTCGACAATCAGAACTCGGGCCATGCt > 6:342364/1‑149 (MQ=255) ccGCGTCGGGCTTGTCCTGCCGGGCCAGGGCCACGCCGTCGGCACCGTTGCTGGCCTTGAGCACCTGGTAGCCGTG‑CTTTTCCAGCCATTCGGTCAATCTGTACATCTCTGTCGGCGAGTCGTCGACAATCAGAACTCGGGCCATGCTg > 6:133205/1‑149 (MQ=255) gCTTGTCCTGCCGGGCCAGGGCCACGCCGTCGGCACCGTTGCTGGCCTTGAGCACCTGGTAGCCGTG‑CTTTTCCAGCCATTCGGTCAATCTGTACATCTCTGTCGGCGAGTCGTCGACAATCAGAACTCGGGCCATGCTGTTTCCCCAt > 6:100595/1‑149 (MQ=255) ttGTCCTGCCGGGCCAGGGCCACGCCGTGGGCACCGTTGCTGGCCTTGAGCACCTGGTAGCCGTG‑CTTTTCCAGCCATTCGGTCAATCTGTACATCTCTGTCGGCGAGTCGTCGACAATCAGAACTCGGGCCATGCTGTTTCCCCAT‑Ca < 7:232638/149‑1 (MQ=255) ttGTCCTGCCGGGCCAGGGCCACGCCGTCGGCACCGTTGCTGGCCTTGAGCACCTGGTAGCCGTG‑CTTTTCCAGCCATTCGGTCAATCTGTACATCTCTGTCGGCGAGTCGTCGACAATCAGAACTCGGGCCATGCTGTTTCCCCAT‑Ca < 1:236739/149‑1 (MQ=255) gTCCTGCCGGGCCAGGGCCACGCCGTCGGCACCGTTGCTGGCCTTGAGCACCTGGTAGCCGTG‑CTTTTCCAGCCATTCGGTCAATCTGTACATCTCTGTCGGCGAGTCGTCGACAATCAGAACTCGGGCCATGCTGTTTCCCCAT‑CAg > 1:73748/1‑148 (MQ=255) tCCTGCCGGGCCAGGGCCACGCCGTCGGCACCGTTGCTGGCCTTGAGCACCTGGTAGCCGTG‑CTTTTCCAGCCATTCGGTCAATCTGTACATCTCTGTCGGCGAGTCGTCGACAATCAGAACTCGGGCCATGCTGTTTCCCCAT‑CAgg > 2:183926/1‑148 (MQ=255) ccTGCCGGGCCAGGGCCACGCCGTCGGCACCGTTGCTGGCCTTGAGCACCTGGTAGCCGTG‑CTTTTCCAGCCATTCGGTCAATCTGTACATCTCTGTCGGCGAGTCGTCGACAATCAGAACTCGGGCCATGCTGTTTCCCCAT‑CAGGa > 7:245224/1‑148 (MQ=255) cTGCCGGGCCAGGGCCACGCCGTCGGCACCGTTGCTGGCCTTGAGCACCTGGTAGCCGTG‑CTTTTCCAGCCATTCGGTCAATCTGTACATCTCTGTCGGCGAGTCGTCGACAATCAGAACTCGGGCCATGCTGTTTCCCCAT‑CAGGaaa < 4:342580/149‑1 (MQ=255) cTGCCGGGCCAGGGCCACGCCGTCGGCACCGTTGCTGGCCTTGAGCACCTGGTAGCCGTG‑CTTTTCCAGCCATTCGGTCAATCTGTACATCTCTGTCGGCGAGTCGTCGACAATCAGAACTCGGGCCATGCTGTTTCCCCAT‑CAGGa > 4:70751/1‑147 (MQ=255) cTGCCGGGCCAGGGCCACGCCGTCGGCACCGTTGCTGGCCTTGAGCACCTGGTAGCCGTG‑CTTTTCCAGCCATTCGGTCAATCTGTACATCTCTGTCGGCGAGTCGTCGACAATCAGAACACGGGCCAGGCTGTTTCCCCAT‑CAGGa > 6:348197/1‑147 (MQ=255) gCCGGGCCAGGGCCACGCCGTCGGCACCGTTGCTGGCCTTGAGCACCTGGTAGCCGTG‑CTTTTCCAGCCATTCGGTCAATCTGTACATCTCTGTCGGCGAGTCGTCGACAATCAGAACTCGGGCCATGCTGTTTCCCCAT‑CAGGAAAg > 5:11386/1‑148 (MQ=255) ccGGGCGAGGGCCACGCCGTCGGCACCGTTGCTGGCCTTGAGCACCTGGTAGCCGTG‑CTTTTCCAGCCATTCGGTCAATCTGTACATCTCTGTCGGCGAGTCGTCGACAATCAGAACTCGGGCCATGCTGTTTCCCCAT‑CAGGa > 5:233935/1‑144 (MQ=255) ccGGGCCAGGGCCACGCCGTCGGCACCGTTGCTGGCCTTGAGCACCTGGTAGCCGTG‑CTTTTCCAGCCATTCGGTCAATCTGTACATCTCTGTCGGCGAGTCGTCGACAATCAGAACTCGGGCCATGCTGTTTCCCCAT‑CAGGa < 6:233935/144‑1 (MQ=255) ccGGGCCAGGGCCACGCCGTCGGCACCGTTGCTGGCCTTGAGCACCTGGTAGCCGTG‑CTTTTCCAGCCATTCGGTCAATCTGTACATCTCTGTCGGCGAGTCGTCGACAATCAGAACTCGGGCCATGCTGTTTCCCCAT‑CAGGAAAGTa < 6:419390/149‑1 (MQ=255) gggccagggccaCGCCGTCGGCACCGTTGCTGGCCTTGAGCACCTGGTAGCCGTG‑CTTTTCCAGCCATTCGGTCAATCTGTACATCTCTGTCGGCGAGTCGTCGACAATCAGAACTCGGGCCATGCTGTTTCCCCAT‑CAGGAAAGTAgg < 5:100595/149‑1 (MQ=255) ggccagggccaCGCCGTCGGCACCGTTGCTGGCCTTGAGCACCTGGTAGCCGTG‑CTTTTCCAGCCATTCGGTCAATCTGTACATCTCTGTCGGCGAGTCGTCGACAATCAGAACTCGGGCCATGCTGTTTCCCCAT‑CAGGAAAGTAGGa < 5:133205/149‑1 (MQ=255) ggccagggccaCGCCGTCGGCACCGTTGCTGGCCTTGAGCACCTGGTAGCCGTG‑CTTTTCCAGCCATTCGGTCAATCTGTACATCTCTGTCGGCGAGTCGTCGACAATCAGAACTCGGGCCATGCTGTTTCCCCAT‑CAGGAAAGTAGGa > 6:289113/1‑149 (MQ=255) gccagggccaCGCCGTCGGCACCGTTGCTGGCCTTGAGCACCTGGTAGCCGTG‑CTTTTCCAGCCATTCGGTCAATCTGTACATCTCTGTCGGCGAGTCGTCGACAATCAGCACTCGGGCCCTGCTGTTTGCCCAG‑CAGGAAAGTAGGAc > 6:132683/1‑149 (MQ=255) ccagggccaCGCCGTCGGCACCGTTGCTGGCCTTGAGCCCCTGGTAGCCGTG‑CTTTTCCAGCCATTCGGTCAATCTGTACATCTCTGTCGGCGAGTCGTCGACAATCAGAACTCGGGCCATGCTGTTTCCCCAT‑CAGGAAAGTAGGAcc > 8:174887/1‑149 (MQ=255) gccaCGCCGTCGGCACCGTTGCTGGCCTTGAGCACCTGGTAGCCGTG‑CTTTTCCAGCCATTCGGTCAATCTGTACATCTCTGTCGGCGAGTCGTCGACAATCAGAACTCGGGCCATGCTGTTTCCCCAT‑CAGGAAAGTAGGACCGCGcc < 5:149040/149‑1 (MQ=255) aCGCCGTCGGCACCGTTGCTGGCCTTGAGCACCTGGTAGCCGTG‑CTTTTCCAGCCATTCGGTCAATCTGTACATCTCTGTCGGCGAGTCGTCGACAATCAGAACTCGGGCCATGCTGTTTCCCCAT‑CAGGAAAGTAGGACCGCGCCATg > 1:216361/1‑149 (MQ=255) aCGCCGTCGGCACCGTTGCTGGCCTTGAGCACCTGGTAGCCGTG‑CTTTTCCAGCCATTCGGTCAATCTGTACATCTCTGTCGGCGAGTCGGCGACAATCAGAACTCGGGCCATGCTGTTTACCCAT‑CAGGAAAGTAGGACCGCGCCATg > 7:307660/1‑149 (MQ=255) gCCGTCGGCACCGTTGCTGGCCTTGAGCACCTGGTAGCCGTGTCTTTTCCAGCCATTCGGTCAATCTGTACATCTCTGTCGGCGAGTCGTCGACAATCAGAACTCGGGCCATGCTGTTTCCCCAT‑CAGGAAAGTAGGACCGCGCCATgg > 1:282038/1‑149 (MQ=255) ggCACCGTTGCTGGCCTTGAGCACCTGGTAGCCGTG‑CTTTTCCAGCCATTCGGTCAATCTGTACATCTCTGTCGGCGAGTCGTCGACAATCAGAACTCGGGCCATGCTGTTTCCCCAT‑CAGGAAAGTAGGACCGCGCCATGGCGGGGCt > 2:227846/1‑149 (MQ=255) cACCGTTGCTGGCCTTGAGCACCTGGTAGCCGTG‑CTTTTCCAGCCATTCGGTCAATCTGTACATCTCTGTCGGCGAGTCGTCGACAATCAGAACTCGGGCCATGCTGTTTCCCCAT‑CAGGAAAGTAGGACCGCGCCATGGCGGGGCTc > 8:111807/1‑148 (MQ=255) cACCGTTGCTGGCCTTGAGCACCTGGTAGCCGTG‑CTTTTCCAGCCATTCGGTCAATCTGTACATCTCTGTCGGCGAGTCGTCGACAATCAGAACTCGGGCCATGCTGTTTCCCCAT‑CAGGAAAGTAGGACCGCGCCATGGCGGGGCTc < 7:111807/148‑1 (MQ=255) cACCGTTGCTGGCCTTGAGCACCTGGTAGCCGTG‑CTTTTCCAGCCATTCGGTCAATCTGTACATCTCTGTCGGCGAGTCGTCGACAATCAGAACTCGGGCCATGCTGTTTCCCCAT‑CAGGAAAGTAGGACCGCGCCATGGCGGGGCTCg < 4:2482/149‑1 (MQ=255) cTGGCCTTGAGCACCTGGTAGCCGTG‑CTTTTCCAGCCATTCGGTCAATCTGTACATCTCTGTCGGCGAGTCGTCGACAATCAGAACTCGGGCCATGCTGTTTCCCCAT‑CAGGAAAGTAGGACCGCGCCATGGCGGGGCTCGGTCAGGGt < 7:164772/149‑1 (MQ=255) cTGGCCTTGAGCACCTGGTAGCCGTG‑CTTTTCCAGCCATTCGGTCAATCTGTACATCTCTGTCGGCGAGTCGTCGACAATCAGAACTCGGGCCATGCTGTTTCCCCAT‑CAGGAAAGTAGGACCGCGCCATGGCGGGGCTCGGTCAGGGt < 5:289113/149‑1 (MQ=255) cTGGCCTTGAGCACCTGGTAGCCGTG‑CTTTTCCAGCCATTCGGTCAATCTGTACATCTCTGTCGGCGAGTCGTCGACAATCAGAACTCGGGCCATGCTGTTTCCCCAT‑CAGGAAAGTAGGACCGCGCCATGGCGGGGCTCGGTCAGGGt > 2:309577/1‑149 (MQ=255) tGGCCTTGAGCACCTGGTAGCCGTG‑CTTTTCCAGCCATTCGGTCAATCTGTACATCTCTGTCGGCGAGTCGTCGACAATCAGAACTCGGGCCATGCTGTTTCCCCAT‑CAGGAAAGTAGGACCGCGCCATGGCGGGGCTCGGTCAGGGTg > 7:374329/1‑149 (MQ=255) gCCTTGAGCACCTGGTAGCCGTG‑CTTTTCCAGCCATTCGGTCAATCTGTACATCTCTGTCGGCGAGTCGTCGACAATCAGAACTCGGGCCATGCTGTTTCCCCAT‑CAGGAAAGTAGGACCGCGCCATGGCGGGGCTCGGTCAGGGTGCg < 3:70751/149‑1 (MQ=255) ccTTGAGCACCTGGTAGCCGTG‑CTTTTCCAGCCATTCGGTCAATCTGTACATCTCTGTCGGCGAGTCGTCGACAATCAGAACTCGGGCCATGCTGTTTCCCCAT‑CAGGAAAGTAGGACCGCGCCATGGCGGGGCTCGGTCAGGGTGCgt > 6:50206/1‑149 (MQ=255) ttGAGCACCTGGTAGCCGTG‑CTTTTCCAGCCATTCGGTCAATCTGTACATCTCTGTCGGCGAGTCGTCGACAATCAGAACTCGGGCCATGCTGTTTCCCCAT‑CAGGAAAGTAGGACCGCGCCATGGCGGGGCTCGGTCAGGGTGCgtgt > 2:183139/1‑149 (MQ=255) ttGAGCACCTGGTAGCCGTG‑CTTTTCCAGCCATTCGGTCAATCTGTACATCTCTGTCGGCGAGTCGTCGACAATCAGAACTCGGGCCATGCTGTTTCCCCAT‑CAGGAAAGTAGGACCGCGCCATGGCGGGGCTCGGTCAGGGTGCgtgt > 1:298416/1‑149 (MQ=255) gAGCACCTGGTAGCCGTG‑CTTTTCCAGCCATTCGGTCAATCTGTACATCTCTGTCGGCGAGTCGTCGACAATCAGAACTCGGGCCATGCTGTTTCCCCAT‑CAGGAAAGTAGGACCGCGCCATGGCGGGGCTCGGTCAGGGTGCGtgtt < 1:227846/148‑1 (MQ=255) aGCACCTGGTAGCCGTG‑CTTTTCCAGCCATTCGGTCAATCTGTACATCTCTGTCGGCGAGTCGTCGACAATCAGAACTCGGGCCATGCTGTTTCCCCAT‑CAGGAAAGTAGGACCGCGCCATGGCGGGGCTCGGTCAGGGTGCGtgttg < 8:370420/148‑1 (MQ=255) gCACCTGGTAGCCGTG‑CTTTTCCAGCCATTCGGTCAATCTGTACATCTCTGTCGGCGAGTCGTCGACAATCAGAACTCGGGCCATGCTGTTTCCCCAT‑CAGGAAAGTAGGACCGCGCCATGGCGGGGCTCGGTCAGGGTGCGTgttgtt < 6:18623/149‑1 (MQ=255) aCCTGGTAGCCGTG‑CTTTTCCAGCCATTCGGTCAATCTGTACATCTCTGTCGGCGAGTCGTCGACAATCAGAACTCGGGCCATGCTGTTTCCCCAT‑CAGGAAAGTAGGACCGCGCCATGGCGGGGCTCGGTCAGGGTGCGTGTTGTTGa < 4:75199/149‑1 (MQ=255) aCCTGGTAGCCGTG‑CTTTTCCAGCCATTCGGTCAATCTGTACATCTCTGTCGGCGAGTCGTCGACAATCAGAACTCGGGCCATGCTGTTTCCCCAT‑CAGGAAAGTAGGACCGCGCCATGGCGGGGCTCGGTCAGGGTGCGTGTTGTTGa < 2:301329/149‑1 (MQ=255) aCCTGGTAGCCGTG‑CTTTTCCAGCCATTCGGTCAATCTGTACATCTCTGTCGGCGAGTCGTCGACAATCAGAACTCGGGCCATGCTGTTTCCCCAT‑CAGGAAAGTAGGACCGCGCCATGGCGGGGCTCGGTCAGGGTGCGTGTTGTTGa < 5:342364/149‑1 (MQ=255) ccTGGTAGCCGTG‑CTTTTCCAGCCATTCGGTCAATCTGTACATCTCTGTCGGCGAGTCGTCGACAATCAGAACTCGGGCCATGCTGTTTCCCCATCCAGGAAAGTAGGACCGCGCCATGGCGGGGCTCGGTCAGGGTGCgtgacgccgc < 1:309577/149‑8 (MQ=255) ccTGGTAGCCGTG‑CTTTTCCAGCCATTCGGTCAATCTGTACATCTCTGTCGGCGAGTCGTCGACAATCAGAACTCGGGCCATGCTGTTTCCCCAT‑CAGGAAAGTAGGACCGCGCCATGGCGGGGCTCGGTCAGGGTGCGTGTTGTTGAg < 1:183926/149‑1 (MQ=255) ccTGGTAGCCGTG‑CTTTTCCAGCCATTCGGTCAATCTGTACATCTCTGTCGGCGAGTCGTCGACAATCAGAACTCGGGCCATGCTGTTTCCCCAT‑CAGGAAAGTAGGACCGCGCCATGGCGGGGCTCGGTCAGGGTGCGTGTTGTTGAg < 3:272499/149‑1 (MQ=255) ccTGGTAGCCGTG‑CTTTTCCAGCCATTCGGTCAATCTGTACATCTCTGTCGGCGAGTCGTCGACAATCAGAACTCGGGCCATGCTGTTTCCCCAT‑CAGGAAAGTAGGACCGCGCCATGGCGGGGCTCGGTCAGGGTGCGTGTTGTTGAg > 3:60534/1‑149 (MQ=255) gTAGCCGTG‑CTTTTCCAGCCATTCGGTCAATCTGTACATCTCTGTTGGCGAGTCGTCGACAATCAGAACTCGGGCCATGCTGTTTCCCCAT‑CAGGAAAGTAGGACCGCGCCATGGCGGGGCTCGGTCAGGGTGCGTGTTGTTGAGGt < 5:245998/147‑1 (MQ=255) gTAGCCGTG‑CTTTTCCAGCCATTCGGTCAATCTGTACATCTCTGTCGGCGCGTCGTCGACAATCAGTACTCGGGCCATGCTGTTTCCCCAT‑CAGGAAAGTAGGACCGCGCCATGGCGGGGCTCGGTCAGGGTGCGTGTTGTTGAGGTGc < 5:348197/149‑1 (MQ=255) tG‑CTTTTCCAGCCATTCGGTCAATCTGTACATCTCTGTCGGCGAGTCGTCGACAATCAGAACTCGGGCCATGCTGTTTCCCCAT‑CAGGAAAGTAGGACCGCGCCATGGCGGGGCTCGGTCAGGGTGCGTGTTGTTGAGGTGCGGCAAAc < 1:183139/149‑1 (MQ=255) tttCCAGCCATTCGGTCAATCTGTACATCTCTGTCGGCGAGTCGTCGACAATCAGAACTCGGGCCATGCTGTTTCCCCAT‑CAGGAAAGTAGGACCGCGCCATGGCGGGGCTCGGTCAGGGTGCGTGTTGTTGAGGTGCGGCAAACCCgg > 8:168198/1‑149 (MQ=255) tttCCAGCCATTCGGTCAATCTGTACATCTCTGTCGGCGAGTCGTCGACAATCAGAACTCGGGCCATGCTGTTTCCCCAT‑CAGGAAAGTAGGACCGCGCCATGGCGGGGCTCGGTCAGGGTGCGTGTTGTTGAGGTGCGGCAAACCCgg > 2:170992/1‑149 (MQ=255) ttCCAGCCATTCGGTCAATCTGTACATCTCTGTCGGCGAGTCGTCGACAATCAGAACTCGGGCCATGCTGTTTCCCCAt < 4:119711/79‑1 (MQ=255) ttCCAGCCATTCGGTCAATCTGTACATCTCTGTCGGCGAGTCGTCGACAATCAGAACTCGGGCCATGCTGTTTCCCCAt > 3:119711/1‑79 (MQ=255) ttCCAGCCATTCGGTCAATCTGTACATCTCTGTCGGCGAGTCGTCGACAATCAGAACTCGGGCCATGCTGTTTCCCCAT‑CAGGAAAGTAGGACCGCGCCATGGCGGGGCTCGGTCAGGGTGCGTGTTGTTGAGGTGCGGCAAACCCg < 6:11386/147‑1 (MQ=255) cAGCCATTCGGTCAATCTGTACATCTCTGTCGGCGAGTCGTCGACAATCAGAACTCGGGCCATGCTGTTTCCCCAT‑CAGGAAAGTAGGACCGCGCCATGGCGGGGCTCGGTCAGGGTGCGTGTTGTTGAGGTGCGGCAAACCCGGGca > 6:167692/1‑148 (MQ=255) aGCCATTCGGTCAATCTGTACATCTCTGTCGGCGAGTCGTCGACAATCAGAACTCGGGCCATGCTGTTTCCCCAT‑CAGGAAAGTAGGACCGCGCCATGGCGGGGCTCGGTCAGGGTGCGTGTTGTTGAGGTGCGGCAAACCCGGGcaca < 6:395118/149‑1 (MQ=255) aGCCATTCGGTCAATCTGTACATCTCTGTCGGCGAGTCGTCGACAATCAGAACTCGGGCCATGCTGTTTCCCCAT‑CAGGAAAGTAGGACCGCGCCATGGCGGGGCTCGGTCAGGGTGCGTGTTGTTGAGGTGCGGCAAACCCGGGcaca > 8:94724/1‑149 (MQ=255) gCCATTCGGTCAATCTGTACATCTCTGTCGGCGAGTCGTCGACAATCAGAACTCGGGCCATGCTGTTTCCCCAT‑CAGGAAAGTAGGACCGCGCCATGGCGGGGCTCGGTCAGGGTGCGTGTTGTTGAGGTGCGGCAAACCCGGGCACAt > 7:324419/1‑149 (MQ=255) ttCGGTCAATCTGTACATCTCTGTCGGCGAGTCGTCGACAATCAGAACTCGGGCCATGCTGTTTCCCCAT‑CAGGAAAGTAGGACCGCGCCATGGCGGGGCTCGGTCAGGGTGCGTGTTGTTGAGGTGCGGCAAACCCGGGCACATgg < 4:297941/147‑1 (MQ=255) ttCGGTCAATCTGTACATCTCTGTCGGCGAGTCGTCGACAATCAGAACTCGGGCCATGCTGTTTCCCCAT‑CAGGAAAGTAGGACCGCGCCATGGCGGGGCTCGGTCAGGGTGCGTGTTGTTGAGGTGCGGCAAACCCGGGCACATgg > 3:297941/1‑147 (MQ=255) ttCGGTCAATCTGTACATCTCTGTCGGCGAGTCGTCGACAATCAGAACTCGGGCCATGCTGTTTCCCCAT‑CAGGAAAGTAGGACCGCGCCATGGCGGGGCGCGGGCCGGGTGCGTGGTGTTGAGGTGCGGCAAACCCGGGcacagtggc > 8:261924/1‑144 (MQ=255) cGGTCAATCTGTACATCTCTGTCGGCGAGTCGTCGACAATCAGAACTCGGGCCATGCTGTTTCCCCAT‑CAGGAAAGTAGGACCGCGCCATGGCGGGGCTCGGTCAGGGTGCGTGTTGTTGAGGTGCGGCAAACCCGGGCACATGGgcgc > 2:166785/1‑149 (MQ=255) gTCAATCTGTACATCTCTGTCGGCGAGTCGTCGACAATCAGAACTCGGGCCATGCTGTTTCCCCAT‑CAGGAAAGTAGGACCGCGCCATGGCGGGGCTCGGTCAGGGTGCGTGTTGTTGAGGTGTGGCAAACCCGGGCACATGGGcgcg > 8:298935/1‑148 (MQ=255) gTCAATCTGTACATCTCTGTCGGCGAGTCGTCGACAATCAGAACTCGGGCCATGCTGTTTCCCCAT‑CAGGAAAGTAGGACCGCGCCATGGCGGGGCTCGGTCAGGGTGCGTGTTGTTGAGGTGCGGCAAACCCGGGCACATGGGCGCgg < 2:282038/149‑1 (MQ=255) gTCAATCTGTACATCTCTGTCGGCGAGTCGTCGACAATCAGAACTCGGGCAATGCTGGTTACACAT‑CAGGAAAGTAGGACCGCGCCATGGCGGGGCTCGGTCAGGCTGCGTGGTGTTGAGGTGCGGCAACCCGGGGCACATGGGcgcg > 8:177367/1‑148 (MQ=255) aaTCTGTACATCTCTGTCGGCGAGTCGTCGACAATCAGAACTCGGGCCATGCTGTTTCCCCAT‑CAGGAAAGTAGGACCGCGCCATGGCGGGGCTCGGTCAGGGTGCGTGTTGTTGAGGTGCGGCAAACCCGGGCACATGGGCGCGGATc < 8:324419/149‑1 (MQ=255) aaTCTGTACATCTCTGTCGGCGAGTCGTCGACAATCAGAACTCGGGCCATGCTGTTTCCCCAT‑CAGGAAAGTAGGACCGCGCCATGGCGGGGCTCGGTCAGGGTGCGTGTTGTTGAGGTGCGGCAAACCCGGGCACATGGGCGCGGATc > 4:4765/1‑149 (MQ=255) aTCTGTACATCTCTGTCGGCGAGTCGTCGACAATCAGAACTCGGGCCATGCTGTTTCCCCAT‑CAGGAAAGTAGGACCGCGCCATGGCGGGGCTCGGTCAGGGTGCGTGTTGTTGAGGTGCGGCAAACCCGGGCACATGGGCGCGGATcg < 1:170992/149‑1 (MQ=255) ctgattGTCGACGACTCGCCGACAATCAGAACTCGGGCCATGCTGTTTCCCCAT‑CAGGAAAGTAGGACCGCGCCATGGCGGGGCTCGGTCAGGGTGCGTGTTGTTGAGGTGCGGCAAACCCGGGCACATgg < 4:219377/126‑1 (MQ=255) ctgattGTCGACGACTCGCCGACAATCAGAACTCGGGCCATGCTGTTTCCCCAT‑CAGGAAAGTAGGACCGCGCCATGGCGGGGCTCGGTCAGGGTGCGTGTTGTTGAGGTGCGGCAAACCCGGGCACATgg > 3:219377/6‑131 (MQ=255) aTCTCTGTCGGCGAGTCGTCGACAATCAGAACTCGGGCCATGCTGTTTCCCCAT‑CAGGAAAGTAGGACCGCGCCATGGCGGGGCTCGGTCAGGGTGCGTGTTGTTGAGGTGCGGCAAACCCGGGCACATGGGCGCGGATCGCGTCGAg > 3:64576/1‑148 (MQ=255) tctcTGTCGGCGAGTCGTCGACAATCAGAACTCGGGCCATGCTGTTTCCCCAT‑CAGTAAAGTAGGACCGCGCCATGGCGGGGCTCGGTCAGGGTGCGTGTTGTTGAGGTGCGGCAAACCCGGGCACATGGGCGCGGATCGCGTCGAg > 1:315639/1‑147 (MQ=255) ctctGTCGGCGAGTCGTCGACAATCAGAACTCGGGCCATGCTGTTTCCCCAT‑CAGGAAAGTAGGACCGCGCCATGGCGGGGCTCGGTCAGGGTGCGTGTTGTTGAGGTGCGGCAAACCCGGGCACATGGGCGCGGATCGCGTCGAGCAg > 1:158456/1‑149 (MQ=255) tGTCGGCGAGTCGTCGACAATCAGAACTCGGGCCATGCTGTTTCCCCAT‑CAGGAAAGTAGGACCGCGCCATGGCGGGGCTCGGTCAGGGTGCGTGTTGTTGAGGTGCGGCAAACCCGGGCACATGGGCGCGGATCGCGTCGAGCAg < 5:150024/146‑1 (MQ=255) tCGGCGAGGCGGCGACAATCAGAACTCGGGCCCTGCTGGTTCCCCAT‑CAGGAAAGTAGGCCCGCGCCATGGCGGGGCTCGGTCAGGGTGCGTGTTGTTGAGGTGCGGCAAACCCGGGCACATGGGCGCGGATCGCGTCGAGCAGttctt < 7:261924/149‑1 (MQ=255) ggCGAGTCGTCGACAATCAGAACTCGGGCCATGCTGTTTCCCCAT‑CAGGAAAGTAGGACCGCGCCATGGCGGGGCTCGGTCAGGGTGCGTGTTGTTGAGGTGCGGCAAACCCGGGCACATGGGCGCGGATCGCGTCGAGCAGttcttc < 3:29762/148‑1 (MQ=255) gCGAGTCGTCGACAATCAGAACTCGGGCCATGCTGTTTCCCCAT‑CAGGAAAGTAGGACCGCGCCATGGCGGGGCTCGGTCAGGGTGCGTGTTGTTGAGGTGCGGCAAACCCGGGCACATGGGCGCGGATCGCGTCGAGCCGTTCTTCCt > 1:138817/1‑149 (MQ=255) gCGAGTCGTCGACAATCAGAACTCGGGCCATGCTGTTTCCCCAT‑CAGGAAAGTAGGACCGCGCCATGGCGGGGCTCGGTCAGGGTGCGTGTTGTTGAGGTGCGGCAAACCCGGGCACATGGGCGCGGATCGCGTCGAGCAGTTCTTCCt < 7:168198/149‑1 (MQ=255) gAGTCGTCGACAATCAGAACTCGGGCCATGCTGTTTCCCCAT‑CAGGAAAGTAGGACCGCGCCATGGCGGGGCTCGGTCAGGGTGCGTGTTGTTGAGGTGCGGCAAACCCGGGGACATGGGCGCGGATGGGGTCGAGCAGTTCTTCCTTg > 8:347418/1‑149 (MQ=255) tcgtcgACAATCAGAACTCGGGCCATGCTGTTTCCCCAT‑CAGGAAAGTAGGACCGCGCCATGGCGGGGCTCGGTCa > 5:224151/1‑76 (MQ=255) tcgtcgACAATCAGAACTCGGGCCATGCTGTTTCCCCAT‑CAGGAAAGTAGGACCGCGCCATGGCGGGGCTCGGTCa < 6:224151/76‑1 (MQ=255) tcgtcgACAATCAGAACTCGGGCCATGCTGTTTCCCCAT‑CAGGAAAGTAGGACCGCGCCATGGCGGGGCTCGGTCAGGGTGCGTGTTGTTGAGGTGGGGCAAACCCGGGCACATGGGCGCGGATCGCGTCGAGCAGTTCTTCCTTGCt < 7:298935/148‑1 (MQ=255) gtcgACAATCAGAACTCGGGCCATGCTGTTTCCCCAT‑CAGGAAAGTAGGACCGCGCCATGGCGGGGCTCGGTCAGGGTGCGTGTTGTTGAGGTGCGGCAAACCCGGGCACATGGGCGCGGATCGCGTCGAGCAGTTCTTCCTTGCTGaa < 8:374329/149‑1 (MQ=255) tcgACAATCAGAACTCGGGCCATGCTGTTTCCCCAT‑CAGGAAAGTAGGACCGCGCCATGGCGGGGCTCGGTCAGGGTGCGTGTTGTTGAGGTGCGGCAAACCCGGGCACATGGGCGCGGATCGCGTCGAGCAGTTCTTCCTTGCTGAAc < 7:174887/149‑1 (MQ=255) | ATGACGATGTCCATCAGCACCGCGTCGGGCTTGTCCTGCCGGGCCAGGGCCACGCCGTCGGCACCGTTGCTGGCCTTGAGCACCTGGTAGCCGTG‑CTTTTCCAGCCATTCGGTCAATCTGTACATCTCTGTCGGCGAGTCGTCGACAATCAGAACTCGGGCCATGCTGTTTCCCCAT‑CAGGAAAGTAGGACCGCGCCATGGCGGGGCTCGGTCAGGGTGCGTGTTGTTGAGGTGCGGCAAACCCGGGCACATGGGCGCGGATCGCGTCGAGCAGTTCTTCCTTGCTGAAC > NC_002947/5689730‑5690019 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |