Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,707,113	G→C	5.5%	L377L (CTG→CTC)	PP_5009 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,707,113	0	G	C	5.5%	236.8 / 3.9	73	L377L (CTG→CTC)	PP_5009	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (30/39);  new base C (2/2);  total (32/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.42e-01

ACCTGCCTTCGCAGGAACGAACTGCGCCCGCTGTCAGCCCACCGCGATGGCTGCTCAATGCCAGCGCTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCCTGCAGACCAGCTATCCAGACAAGGCAGTACACGACCCGAACGATGTGCACATCCGCATTTCCCAAGCCATCATCGGCGCCGGCACACAAGGGCA  >  NC_002947/5706968‑5707259                                                                                                                                                  |                                                                                                                                                   aCCTGCCTTCGCAGGAACGAACTGCGCCCGCTGTCAGCCCACCGCGATGGCTGCTCAATGCCAGCGCTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGc                                                                                                                                                 >  1:74007/1‑149 (MQ=255)     gCCTTCGCAGGAACGAACTGCGCCCGCTGTCAGCCCACCGCGATGGCTGCTCAATGCCAGCGCTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTgg                                                                                                                                                   >  8:13664/1‑143 (MQ=255)     gCCTTCGCAGGAACGAACTGCGCCCGCTGTCAGCCCACCGCGATGGCTGCTCAATGCCAGCGCTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTgg                                                                                                                                                   <  7:13664/143‑1 (MQ=255)      ccTTCGCAGGAACGAACTGCGCCCGCTGTCAGCCCACCGCGATGGCTGCTCAATGCCAGCGCTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGAt                                                                                                                                              <  6:411224/147‑1 (MQ=255)          cGCAGGAACGAACTGCGCCCGCTGTCAGCCCACCGCGATGGCTGCTCAATGCCAGCGCTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTCGAAGGGCTGCACATCGCCCAGCGATGtgc                                                                                                                                          <  2:185662/147‑3 (MQ=255)           gCAGGAACGAACTGCGCCCGCTGTCAGCCCACCGCGATGGCTGCTCAATGCCAGCGCTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCg                                                                                                                                          >  4:16846/1‑146 (MQ=255)           gCAGGAACGAACTGCGCCCGCTGTCAGCCCACCGCGATGGCTGCTCAATGCCAGCGCTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGaa                                                                                                                                        <  2:74007/148‑1 (MQ=255)           gCAGGAACGAACTGCGCCCGCTGTCAGCCCACCGCGATGGCTGCTCAATGCCAGCGCTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGaa                                                                                                                                        <  4:67436/148‑1 (MQ=255)           gCAGGAACGAACTGCGCCCGCTGTCAGCCCACCGCGATGGCTGCTCAATGCCAGCGCTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGaa                                                                                                                                        <  7:10031/148‑1 (MQ=255)            cAGGAACGAACTGCGCCCGCTGTCAGCCCACCGCGATGGCTGCTCAATGCCAGCGCTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGa                                                                                                                                      <  3:16846/149‑1 (MQ=255)                     acTGCGCCCGCTGTCAGCCCACCGCGATGGCTGCTCAATGCCAGCGCTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGa                                                                                                                             >  1:9303/1‑149 (MQ=255)                         cgcCCGCTGTCAGCCCACCGCGATGGCTGCTCAATGCCAGCGCTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGACGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTAc                                                                                                                         >  5:63780/1‑149 (MQ=255)                                tGTCAGCCCACCGCGATGGCTGCTCAATGCCAGCGCTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTAcgccgctc                                                                                                                  >  6:164460/1‑149 (MQ=255)                                     gCCCACCGCGATGGCTGCTCAATGCCAGCGCTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTAcgccgctcgccgc                                                                                                             <  6:75233/149‑1 (MQ=255)                                       ccACCGCGATGGCTGCTCAATGCCAGCGCTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCtt                                                                                                           <  8:221754/149‑1 (MQ=255)                                        cACCGCGATGGCTGCTCAATGCCAGCGCTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTAcgc                                                                                                                       <  4:318696/136‑1 (MQ=255)                                        cACCGCGATGGCTGCTCAATGCCAGCGCTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTAcgc                                                                                                                       >  3:318696/1‑136 (MQ=255)                                         aCCGCGATGGCTGCTCAATGCCAGCGCTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGc                                                                                                         >  4:90085/1‑149 (MQ=255)                                            gcgATGGCTGCTCAATGCCAGCGCTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTg                                                                                                      <  2:408518/149‑1 (MQ=255)                                               aTGGCTGCTCAATGCCAGCGCTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTgaga                                                                                                   <  2:9303/149‑1 (MQ=255)                                                  gctgctCAATGCCAGCGCTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGc                                                                                                         <  2:89542/140‑1 (MQ=255)                                                  gctgctCAATGCCAGCGCTGAGGACCGCTACGCCTATCACACGGCAATGCTCGATCTGGCGGCGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGc                                                                                                         >  1:89542/1‑140 (MQ=255)                                                    tgctCAATGCCAGCGCTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCTGTCGAAGGGCTGCACATCGCTGCGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCATg                                                                                              >  2:339262/1‑149 (MQ=255)                                                      ctCAATGCCAGCGCTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCACGGTCGGTCGAAGGGCGGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCCTg                                                                                              <  1:83296/147‑1 (MQ=255)                                                      ctCAATGCCAGCGCTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCCTg                                                                                              <  3:203013/147‑1 (MQ=255)                                                      ctCAATGCCAGCGCTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCCTg                                                                                              <  2:313816/147‑1 (MQ=255)                                                           tGCCAGCGCTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCCTGCAGACCa                                                                                       <  2:12684/149‑1 (MQ=255)                                                              cAGCGCTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCCTg                                                                                              <  3:211542/139‑1 (MQ=255)                                                              cAGCGCTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCCTg                                                                                              >  4:211542/1‑139 (MQ=255)                                                                 cgcTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCCTGCAGACCAGCTATc                                                                                 <  1:152263/149‑1 (MQ=255)                                                                 cgcTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCCTGCAGACCAGCTATc                                                                                 >  4:40814/1‑149 (MQ=255)                                                                   cTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTAcgccgctcgc                                                                                                                >  6:75297/1‑116 (MQ=255)                                                                   cTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTAcgccgctcgc                                                                                                                <  5:75297/116‑1 (MQ=255)                                                                     gAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCCTGCAGACCa                                                                                       <  8:193265/139‑1 (MQ=255)                                                                     gAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCCTGCAGACCa                                                                                       >  7:193265/1‑139 (MQ=255)                                                                      aGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCCTGCAGACCAGCTATCCAGa                                                                             >  5:239050/1‑148 (MQ=255)                                                                       ggACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCCTGCAGACCAGCTATCCAGAc                                                                            >  4:342073/1‑148 (MQ=255)                                                                        gACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCCTGCa                                                                                            >  8:185529/1‑131 (MQ=255)                                                                        gACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCCTGCa                                                                                            <  7:185529/131‑1 (MQ=255)                                                                         aCCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCCTGCAGACCAGCTATCCAGACAAg                                                                         <  7:300392/149‑1 (MQ=255)                                                                                cGCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCCTGCAGACCAGCTATCCAGACAAGGCAGt                                                                    <  2:258285/147‑1 (MQ=255)                                                                                cGCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCCTGCAGACCAGCTATCCAGACAAGGCAGt                                                                    <  5:164460/147‑1 (MQ=255)                                                                                    tATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCCTGCAGACCAGCTATCCAGACAAGGCAGTACACGa                                                              >  8:211451/1‑149 (MQ=255)                                                                                     aTCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCCTGCAGACCAGCTATCCAGACAAGGCAGTACACGAc                                                             >  8:49239/1‑149 (MQ=255)                                                                                     aTCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCCTGCAGACCAGCTATCCAGACAAGGCAGTACACGAc                                                             >  7:272842/1‑149 (MQ=255)                                                                                        acacGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCCTGCAGACCAGCTATCCAGACAAGGCAGTACACGa                                                              <  6:265278/145‑1 (MQ=255)                                                                                        acacGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCCTGCAGACCAGCTATCCAGACAAGGCAGTACACGa                                                              >  5:265278/1‑145 (MQ=255)                                                                                          acGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGAACCTGCAGACCAGCTATCCAGACAAGGCAGTACACGACCCGaa                                                        >  5:86366/1‑149 (MQ=255)                                                                                              cACTGCTCGATCTGGCGGCGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCCTGCAGACCAGCTATCCAGACAAGGCAGTACACGACCCGAACGAt                                                    <  7:211451/149‑1 (MQ=255)                                                                                                cTGCTCGATCTGGCGGCGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCCTGCAGACCAGCTATCCAGACAAGGCAGTACACGACCCGAACGAtgt                                                  <  3:342073/149‑1 (MQ=255)                                                                                                    tCGATCTGGCGGCGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCCTGCAGACCAGCTATCCAGACAAGGCAGTaca                                                                 <  8:341995/130‑1 (MQ=255)                                                                                                    tCGATCTGGCGGCGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCCTGCAGACCAGCTATCCAGACAAGGCAGTaca                                                                 >  7:341995/1‑130 (MQ=255)                                                                                                      gATCTGGCGGCGAACCATGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCCTGCAGACCAGCTATCCAGACAAGGc                                                                       >  6:371786/1‑122 (MQ=255)                                                                                                      gATCTGGCGGCGAACCATGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCCTGCAGACCAGCTATCCAGACAAGGc                                                                       <  5:371786/122‑1 (MQ=255)                                                                                                            gcggcgAACCAAGGTCGGACGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCCTGCAGACCAGCTATCCAGACAAGGCAGTACACGACCCGAACGATGTGCACATCCGCAt                                      <  6:63780/149‑1 (MQ=255)                                                                                                              ggcgAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCCTGCAGACCAGCTATCCAGACAAGGCAGTACACGACCCGAACGAt                                                    >  1:322155/1‑133 (MQ=255)                                                                                                              ggcgAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCCTGCAGACCAGCTATCCAGACAAGGCAGTACACGACCCGAACGAt                                                    <  2:322155/133‑1 (MQ=255)                                                                                                                 gAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCCTGCAGACCAGCTATCCAGACAAGGCAGTACACGACCCGAACGATGTGCACATCCGCATTTccc                                 <  3:90085/149‑1 (MQ=255)                                                                                                                  aaCCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCCTGCAGACCAGCTATCCAGACAAGGCAGTACACGACCCGAACGATGTGCACATCCGCATTTccc                                 >  1:336431/1‑148 (MQ=255)                                                                                                                   aCCAAGGTCGGTCGAAGGGTTGCACATCGGTGGGCTATGTCAGCGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCCTGCAGACCAGCTATCCAGAGAAGGCAGTACACGACCCGAACGATGTGCACATCCGCATTTCCCaa                               <  1:339262/149‑1 (MQ=255)                                                                                                                   aCCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCCTGCAGACCAGCTATCCAGACAAGGCAGTACACGACCCGAACGATGTGCACATCCGCATTTccc                                 >  3:275616/1‑147 (MQ=255)                                                                                                                      aaGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCCTGCAGACCAGCTATCCAGACAAGGCAGTACACGACCCGAACGATGTGCACATCCGCATTTc                                   >  6:6821/1‑142 (MQ=255)                                                                                                                      aaGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCCTGCAGACCAGCTATCCAGACAAGGCAGTACACGACCCGAACGATGTGCACATCCGCATTTc                                   <  5:6821/142‑1 (MQ=255)                                                                                                                       aGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCCTGCAGACCAg                                                                                      <  7:306551/90‑1 (MQ=255)                                                                                                                       aGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCCTGCAGACCAg                                                                                      >  8:306551/1‑90 (MQ=255)                                                                                                                         gtcggtcgAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCCTGCAGACCAGCTATCCAGACAAGGCAGTACACGACCCGAACGATGTGCACATCCGCATTTCCCAAGCcatc                         <  6:239050/149‑1 (MQ=255)                                                                                                                             gtcgAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCCTGCAGACCAGCTATCCAGACAAGGCAGTACACGACCCGAACGATGTGCACATCCGCATTTCCCAAGCCATCATCg                     <  3:40814/149‑1 (MQ=255)                                                                                                                              tcgAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCCTGCAGACCAGCTATCCAGACAAGGCAGTACACGACCCGAACGATGTGCACATCCGCATTTCCCAAGCCATCATCgg                    >  1:732/1‑149 (MQ=255)                                                                                                                                   gggCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCCTGCAGACCAGCTATCCAGACAAGGCAGTACACGACCCGAACGATGTGCACATCCGCATTTCCCAAGCCATCATCGGCGcc                >  1:245920/1‑148 (MQ=255)                                                                                                                                       tcgacaTCGCCCAGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCCTGCAGACCAGCTATCCAGACAAGGCAGTACACGACCCGAACGATGTGCACATCCGCa                                       <  6:176258/118‑1 (MQ=255)                                                                                                                                       tcgacaTCGCCCAGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCCTGCAGACCAGCTATCCAGACAAGGCAGTACACGACCCGAACGATGTGCACATCCGCa                                       >  5:176258/4‑121 (MQ=255)                                                                                                                                        cgacaTCGCCCAGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCCTGCAGACCAGCTATCCAGACAAGGCAGTACACGACCCGAACGATGTGCACATCCGCATTTCCCAAGCCATCATCGGCGCCGGcaca          >  8:218460/3‑149 (MQ=255)                                                                                                                                         cacaTCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCCTGCAGACCAGCTATCCAGACAAGGCAGTACACGACCCGAACGATGTGCACATCCGCATTTCCCAAGCCATCATCGGCGCCGGcac           <  4:204686/147‑1 (MQ=255)                                                                                                                                             tCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCCTGCAGACCAGCTATCCAGACAAGGCAGTACACGACCCGAACGATGTGCACATCCGCATTTCCCAAGCCATCATCGGCGCCGGCACACAAgg     <  6:86366/149‑1 (MQ=255)                                                                                                                                                cTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCCTGCAGACCAGCTATCCAGACAAGGCAGTACACGACCCGAACGATGTGCACATCCGCATTTCCCAAGCCATCATCGGCGCCGGCACACAAGGGCa  <  8:272842/149‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                                   ACCTGCCTTCGCAGGAACGAACTGCGCCCGCTGTCAGCCCACCGCGATGGCTGCTCAATGCCAGCGCTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCCTGCAGACCAGCTATCCAGACAAGGCAGTACACGACCCGAACGATGTGCACATCCGCATTTCCCAAGCCATCATCGGCGCCGGCACACAAGGGCA  >  NC_002947/5706968‑5707259

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑