Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	474,163	G→C	5.7%	A274A (GCC→GCG)	dnaG ←	DNA primase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	474,163	0	G	C	5.7%	275.0 / 3.5	85	A274A (GCC→GCG)	dnaG	DNA primase
Reads supporting (aligned to +/- strand):  ref base G (36/44);  new base C (2/3);  total (38/47)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTGCGCGCCAGGCGGCCTTGCGCCCGGCCTGGTCGCCGTCAAAACAGAACAGCACGCTGGGCACCACGCGGAACAGGCGCTTGAGGTGCTCTTCGCTGGTGGCGGTGCCAAGGGTGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGCCTCGTACAGCCCGTACAGCTCCTGCCCCTTGTGGAAAACCGGGGTTTCCGGGGAGTTCAGGTACTTGGGCTTGTCGTCGCCGA  >  NC_002947/474015‑474311                                                                                                                                                     |                                                                                                                                                      gTGCGCGCCAGGCGGCCTTGCGCCCGGCCTGGTCGCCGTCAAAACAGAACAGCACGCTGGGCACCACGCGGAACAGGCGCTTGAGGTGCTCTTCGCTGGTGGCGGTGCCAAGGGTGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAgg                                                                                                                                                       <  4:152629/149‑1 (MQ=255)  tGCGCGCCAGGCGGCCTTGCGCCCGGCCTGGTCGCCGTCAAAACAGAACAGCACGCTGGGCACCACGCGGAACAGGCGCTTGAGGTGCTCTTCGCTGGTGGCGGTGCCAAGGGTGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAggg                                                                                                                                                      <  5:175024/149‑1 (MQ=255)  tGCGCGCCAGGCGGCCTTGCGCCCGGCCTGGTCGCCGTCAAAACAGAACAGCACGCTGGGCACCACGCGGAACAGGCGCTTGAGGTGCTCTTCGCTGGTGGCGGTGCCAAGGGTGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAggg                                                                                                                                                      <  5:348496/149‑1 (MQ=255)      ctccAGGCGTCCTTGCGCCCGGCCTGGTCGCCGTCAAAACAGAAGAGCACGCTGGGCAACACGCGGAACAGGCGCTTGAGGTGCTCTTCGCTGGTGGCGGTGCCAAGGGTGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAAt                                                                                                                                                  <  8:125120/147‑1 (MQ=255)       gcCAGGCGGCCTTGCGCCCGGCCTGGTCGCCGTCAAAACAGAACAGCACGCTGGGCACCACGCGGAACAGGCGCTTGAGGTGCTCTTCGCTGGTGGCGGTGCCAAGGGTGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAAt                                                                                                                                                  >  3:119184/1‑148 (MQ=255)       gcCAGGCGGCCTTGCGCCCGGCCTGGTCGCCGTCAAAACAGAACAGCACGCTGGGCACCACGCGGAACAGGCGCTTGAGGTGCTCTTCGCTGGTGGCGGTGCCAAGGGTGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATg                                                                                                                                                 >  3:309644/1‑149 (MQ=255)       gcCAGGCGGCCTTGCGCCCGGCCTGGTCGCCGTCAAAACAGAACAGCACGCTGGGCACCACGCGGAACAGGCGCTTGAGGTGCTCTTCGCTGGTGGCGGTGCCAAGGGTGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATg                                                                                                                                                 <  3:286827/149‑1 (MQ=255)       gcCAGGCGGCCTTGCGCCCGGCCTGGTCGCCGTCAAAACAGAACAGCACGCTGGGCACCACGCGGAACAGGCGCTTGAGGTGCTCTTCGCTGGTGGCGGTGCCAAGGGTGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATg                                                                                                                                                 <  7:279714/149‑1 (MQ=255)       gcCAGGCGGCCTTGCGCCCGGCCTGGTCGCCGTCAAAACAGAACAGCACGCTGGGCACCACGCGGAACAGGCGCTTGAGGTGCTCTTCGCTGGTGGCGGTGCCAAGGGTGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATg                                                                                                                                                 >  5:29807/1‑149 (MQ=255)         cAGGCGGCCTTGCGCCCGGCCTGGTCGCCGTCAAAACAGAACAGCACGCTGGGCACCACGCGGAACAGGCGCTTGAGGTGCTCTTCGCTGGTGGCGGTGCCAAGGGTGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGAc                                                                                                                                               <  4:119184/149‑1 (MQ=255)          aGGCGGCCTTGCGCCCGGCCTGGTCGCCGTCAAAACAGAACAGCACGCTGGGCACCACGCGGAACAGGCGCTTGAGGTGCTCTTCGCTGGTGGCGGTGCCAAGGGTGGCCACGTCATTGCCCTGGCCCAGCAAGGCCTGCGCAATGCCg                                                                                                                                              <  4:324392/149‑1 (MQ=38)           ggcggcCTTGCGCCCGGCCTGGTCGCCGTCAAAACAGAACAGCACGCTGGGCACCACGCGGAACAGGCGCTTGAGGTGCTCTTCGCTGGTGGCGGTGCCAAGGGTGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGt                                                                                                                                             <  5:17782/149‑1 (MQ=255)           ggcggcCTTGCGCCCGGCCTGGTCGCCGTCAAAACAGAACAGCACGCTGGGCACCACGCGGAACAGGCGCTTGAGGTGCTCTTCGCTGGTGGCGGTGCCAAGGGTGGCCACGGCATTGCCCTGGCCCAGCAAGGCCTGCGCAATGCCGt                                                                                                                                             <  7:242333/149‑1 (MQ=38)               gcCTTGCGCCCGGCCTGGTCGCCGTCAAAACAGAACAGCACGCTGGGCACCACGCGGAACAGGCGCTTGAGGTGCTCTTCGCTGGTGGCGGTGCCAAGGGTGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCAt                                                                                                                                         <  7:126812/149‑1 (MQ=255)                ccTTGCGCCCGGCCTGGTCGCCGTCAAAACAGAACAGCACGCTGGGCACCACGCGGAACAGGCGCTTGAGGTGCTCTTCGCTGGTGGCGGTGCCAAGGGTGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATg                                                                                                                                        <  5:365647/149‑1 (MQ=255)                 cTTGCGCCCGGCCTGGGCGCCGTCAAAACAGAACAGCCCGCTGGGCACCACGCGGAACAGGCGCTTGAGGTGCTCTTCGCTGGTGGCGGTGCCAAGGGTGGCCACGGCATTGCGCAGGCCTAGCTGGGCCAGGGCAATGACGTCCATGt                                                                                                                                       <  1:225174/149‑1 (MQ=255)                  ttGCGCCCGGCCTGGTCGCCGTCAAAACAGAACAGCACGCTGGGCACCACGCGGAACAGGCGCTTGAGGTGCTCTTCGCTGGTGGCGGTGCCAAGGGTGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATg                                                                                                                                        >  6:268682/1‑147 (MQ=255)                      gcCCGGCCTGGTCGCCGTCAAAACAGAACAGCACGCTGGGCACCACGCGGAACAGGCGCTTGAGGTGCTCTTCGCTGGTGGCGGTGCCAAGGGTGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGccc                                                                                                                                  >  6:387763/1‑149 (MQ=255)                       cccGGCCTGGTCGCCGTCAAAACAGAACAGCACGCTGGGCACCACGCGGAACAGGCGCTTGAGGTGCTCTTCGCTGGTGGCGGTGCCGAGGGTGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCt                                                                                                                                 >  7:188834/1‑149 (MQ=255)                        ccGGCCTGGTCGCCGTCAAAACAGAACAGCACGCTGGGCACCACGCGGAACAGGCGCTTGAGGTGCTCTTCGCTGGTGGCGGTGCCAAGGGTGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTc                                                                                                                                <  4:56457/149‑1 (MQ=255)                                   gCCGTCAAAACAGAACAGCACGCTGGGCACCACGCGGAACAGGCGCTTGAGGTGCTCTTCGCTGGTGGCGGTGCCAAGGGTGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACgatga                                                                                                                     >  7:355079/1‑149 (MQ=255)                                   gCCGTCAAAACAGAACAGCACGCTGGGCACCACGCGGAACAGGCGCTTGAGGTGCTCTTCGCTGGTGGCGGTGCCAAGGGTGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACgatga                                                                                                                     >  4:33970/1‑149 (MQ=255)                                         aaaaCAGAACAGCACGCTGGGCACCACGCGGAACAGGCGCTTGAGGTGCTCTTCGCTGGTGGCGGTGCCAAGGGTGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGcc                                                                                                                                   >  4:257330/1‑129 (MQ=255)                                         aaaaCAGAACAGCACGCTGGGCACCACGCGGAACAGGCGCTTGAGGTGCTCTTCGCTGGTGGCGGTGCCAAGGGTGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGcc                                                                                                                                   <  3:257330/129‑1 (MQ=255)                                          aaaCAGAACAGCACGCTGGGCACCACGCGGAACAGGCGCTTGAGGTGCTCTTCGCTGGTGGCGGTGCCAAGGGTGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑tcgtc                                                                                                             <  6:418929/149‑1 (MQ=255)                                            acagaacagCACGCTGGGCACCACGCGGAACAGGCGCTTGAGGTGCTCTTCGCTGGTGGCGGTGCCAAGGGTGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑tcgtc                                                                                                             >  8:254573/1‑147 (MQ=255)                                            acagaacagCACGATGGGCACCACGCGGAACAGGCGCTTGAGGAGCTCTTCGCTGGTGGCGGTGCCAAGGGTGGACACGGCATTCCGCAGGCATTGCTGGGACAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑tcgtc                                                                                                             >  4:40761/1‑147 (MQ=255)                                              agaacagCTCGCTGGGCACCACGCGGAACAGGCGGTTGAGGTGCTCTTCGCTGGTGGCGGTGCCAAGGGTGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAgg                                                                                                         <  1:403219/149‑1 (MQ=255)                                                aacagCACGCTGGGCACCACGCGGAACAGGCGCTTGAGGTGCTCTTCGCTGGTGGCGGTGCCAAGGGTGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACgat                                                                                                                       <  4:365021/134‑1 (MQ=255)                                                aacagCACGCTGGGCACCACGCGGAACAGGCGCTTGAGGTGCTCTTCGCTGGTGGCGGTGCCAAGGGTGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACgat                                                                                                                       >  3:365021/1‑134 (MQ=255)                                                aacagCACGCTGGGCACCACGCGGAACAGGCGCTTGAGGTGCTCTTCGCTGGTGGCGGTGCCAAGGGTGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGtt                                                                                                       >  5:388809/1‑149 (MQ=255)                                                 acagCACGCTGGGCACCACGCGGAACAGGCGCTTGAGGTGCTCTTCGCTGGTGGCGGTGCCAAGGGTGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGA‑GATCAtcgtcgtcgagg                                                                                                      <  1:27392/149‑7 (MQ=255)                                                  cagCACGCTGGGCACCACGCGGAACAGGCGCTTGAGGTGCTCTTCGCTGGTGGCGGTGCCGAGGGTGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGc                                                                                                     <  8:188834/149‑1 (MQ=255)                                                  cagCACGCTGGGCACCACGCGGAACAGGCGCTTGAGGTGCTCTTCGCTGGTGGCGGTGCCAAGGGTGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGc                                                                                                     >  8:192279/1‑149 (MQ=255)                                                    gCACGCTGGGCACCACGCGGAACAGGCGCTTGAGGTGCTCTTCGCTGGTGGCGGTGCCAAGGGTGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCgg                                                                                                   <  7:192279/149‑1 (MQ=255)                                                     cACGCTGGGCACCACGCGGAACAGGCGCTTGAGGTGCTCTTCGCTGGTGGCGGTGCCAAGGGTGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGt                                                                                                  >  3:30928/1‑149 (MQ=255)                                                     cACGCTGGGCACCACGCGGAACAGGCGCTTGAGGTGCTCTTCGCTGGTGGCGGTGCCAAGGGTGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGt                                                                                                  >  3:25539/1‑149 (MQ=255)                                                     cACCCTGGCCACCACGCGGAACAGCCCATTGAGCTGCTCTTCCATCGTGTCGGTGCCAAGGGTCGCCACGGCATTGAGCACCCCTTGCTGGGCCAGCGCAAACACGTCCATCTAGCCCTCGACGACGATGATC‑TCGTCGAGCTTCCgct                                                                                                  >  4:381236/1‑147 (MQ=255)                                                      aCGCTGGGCACCACGCGGAACAGGCGCTTGAGGTGCTCTTCGCTGGTGGCGGTGCCAAGGGTGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGtt                                                                                                 <  5:268682/149‑1 (MQ=255)                                                        gCTGGGCACCACGCGGAACAGGCGCTTGAGGTGCTCTTCGCTGGTGGCGGTGCCAAGGGTGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTtgt                                                                                               <  3:33970/149‑1 (MQ=255)                                                        gCTGGGCACCACGCGGAACAGGCGCTTGAGGTGCTCTTCGCTGGTGGCGGTGCCAAGGGTGGCCACGGCATTGCGCAGGCCTTACTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTtgt                                                                                               >  1:213896/1‑149 (MQ=255)                                                            ggCACCACGCGGAACAGGCGCTTGAGGTGCTCTTCGCTGGTGGCGGTGCCAAGGGTGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCtt                                                                                           <  4:309644/149‑1 (MQ=255)                                                            ggCACCACGCGGAACAGGCGCTTGAGGTGCTCTTCGCTGGTGGCGGTGCCAAGGGTGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCtt                                                                                           <  8:184163/149‑1 (MQ=255)                                                              caccacGCGGAACAGGCGCTTGAGGTGCTCTTCGCTGGTGGCGGTGCCAAGGGTGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCTTgc                                                                                         <  2:52550/149‑1 (MQ=255)                                                                  acGCGGAACAGGCGCTTGAGGTGCTCTTCGCTGGTGGCGGTGCCAAGGGTGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCTTgcgcgc                                                                                     <  2:310501/149‑1 (MQ=255)                                                                      ggAACAGGCGCTTGAGGTGCTCTTCGCTGGGGGCGGTGCCAAGGGTGGCCACGGCATTGCGCAGGCCTTGCTGAGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGCCTCg                                                                                 <  8:165609/149‑1 (MQ=255)                                                                          cAGGCGCTTGAGGTGCTCTTCGCTGGTGGCGGTGCCAAGGGTGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGCCTCGTa                                                                               >  2:239662/1‑147 (MQ=255)                                                                               gcTTGAGGTGCTCTTCGCTGGTGGCGGTGCCAAGGGTGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGCCTCGTACAGCCCg                                                                        >  3:81372/1‑149 (MQ=255)                                                                                    aGGTGCTCTTCGCTGGTGGCGGTGCCAAGGGTGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGCCTCGTACAGCCCGTACAg                                                                   >  3:259945/1‑149 (MQ=255)                                                                                      gTGCTCTTCGCTGGTGGCGGTGCCAAGGGTGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGCCTCGTACAGCCCGTACAGCt                                                                 >  6:7974/1‑149 (MQ=255)                                                                                      gTGCTCTTCGCTGGTGGCGGTGCCAAGGGTGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGAAGACAACGACGACAAAGATC‑TCGTCGAGGTTGAGGTTGTGATTGCGCGCCTCGTAAAGCCCGTACAGCt                                                                 >  1:334250/1‑149 (MQ=255)                                                                                              cGCTGGTGGCGTTGCCAAGTGTGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGCCTCGTACAGCCCGTACAGCTCCTGcc                                                           <  5:404625/147‑1 (MQ=255)                                                                                              cGCTGGTGGCGGTGCCAAGGGTGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGCCTCGTACAGCCCGTACAGCTCCTGc                                                            <  4:87279/146‑1 (MQ=255)                                                                                               gCTGGTGGCGGTGCCAAGGGTGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGcc                                                                                    >  8:92461/1‑121 (MQ=255)                                                                                               gCTGGTGGCGGTGCCAAGGGTGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGcc                                                                                    <  7:92461/121‑1 (MQ=255)                                                                                               gCTGGTGGCGGTGCCAAGGGTGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGCCTCGTACAGCCCGTACAGCTCCTGCCCCt                                                        >  7:260990/1‑149 (MQ=255)                                                                                                   gtggCGGTGCCAAGGGTGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGCCTCGTACAGCCCGTACAGCTCCTGCCCCTtgt                                                     <  1:298139/148‑1 (MQ=255)                                                                                                    tggCGGTGCCAAGGGTGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGCCTCGTACAGCCCGTACAGCTCCTGCCCCTtgtg                                                    >  2:82532/1‑148 (MQ=255)                                                                                                      gCGGTGCCAAGGGTGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGCCTCGTACAGCCCGTACAGCTCCTGCCCCTTGTGGa                                                  <  5:387763/148‑1 (MQ=255)                                                                                                      gCGGTGCCAAGGGTGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGCCTCGTACAGCCCGTACAGCTCCTGCCCCTTGTGGa                                                  >  4:138626/1‑148 (MQ=255)                                                                                                        ggTGCCAAGGGTGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGCCTCGTACAGCCCGTACAGCTCCTGCCCCTTGTGGaaaa                                               >  5:276374/1‑149 (MQ=255)                                                                                                            ccAAGGGTGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGCCTCGTACAGCCCGTACAGCTCCTGCCCCTTGTGGAAAACCg                                            <  6:29807/148‑1 (MQ=255)                                                                                                             ccaGGGTGGCCACGGCATAGCGCAGGCCGTGCGGGGCCAGCGCAATGACGTCCATGTAGCCCTCGACGACGAGGATC‑TCGTCGAGGTTGCGGTTGTGCTGGCGCGCCTCGTACAGCCCGGACAGCTCCTGCCCCTTGTGGAAACCCggg                                          <  8:355079/147‑1 (MQ=255)                                                                                                             cAAGGGTGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGCCTCGTACAGCCCGTACAGCTCCTGCCCCTTGTGGAAAACCggg                                          <  4:30928/149‑1 (MQ=255)                                                                                                             cAAGGGTGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGCCTCGTACAGCCCGTACAGCTCCTGCCCCTTGTGGAAAACCggg                                          <  1:239662/149‑1 (MQ=255)                                                                                                             cAAGGGTGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGCCTCGTACAGCCCGTACAGCTCCTGCCCCTTGTGGAAAACCggg                                          <  8:248086/149‑1 (MQ=255)                                                                                                             cAAGGGTGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGCCTCGTACAGCCCGTACAGCTCCTGCCCCTTGTGGAAAACCggg                                          <  4:25539/149‑1 (MQ=255)                                                                                                                     gacACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGCCGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGCCTCGTACAGCCCGTACAGCTCCTGCCCCTTGTGGAAAACCGGGGTTTCCgg                                  <  3:138626/147‑1 (MQ=255)                                                                                                                     gCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGCCTCGTACAGCCCGTACAGGTCCTGCCGCTTGTGGAAAACCGGTGTTTCCg                                   <  3:186275/148‑1 (MQ=255)                                                                                                                      ccACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGCCTCGTACAGCCCGTACAGCTCCTGCCCCTTGTGGAAAACCGGGGTTTCCggg                                 <  6:388803/149‑1 (MQ=255)                                                                                                                      ccACGGCATTGCGCAGGCCTTACTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGCCTCGTACAGCCCGTACAGCTCCTGCCCCTTGTGGAAAACCGGGGTTTCCggg                                 <  2:213896/149‑1 (MQ=255)                                                                                                                       cACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGCCTCGTACAGCCCGTACAGCTCCTGCCCCTTGTGGAAAACCggg                                          >  6:311807/1‑139 (MQ=255)                                                                                                                       cACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGCCTCGTACAGCCCGTACAGCTCCTGCCCCTTGTGGAAAACCggg                                          <  5:311807/139‑1 (MQ=255)                                                                                                                       cACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGCCTCGTACAGCCCGTACAGCTCCTGCCCCTTGTGGAAAACCGGGGTTTCCggg                                 >  4:382704/1‑148 (MQ=255)                                                                                                                       cACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGCCTCGTACAGCCCGTACAGCTCCTGCCCCTTGTGGAAAACCGGGGTTTCCggg                                 >  1:379420/1‑148 (MQ=255)                                                                                                                       cACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGCCTCGTACAGCCCGGACAGCTCCTGCCCCTTGTGGAAAACCGGGGTTTCCggg                                 >  1:376148/1‑148 (MQ=255)                                                                                                                       cACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGAGCTTGCGCGCCTCGTACAGCCCGTACAGCTCCTGCCCCTTGTGGAAAACCGGGGTTTCCggg                                 >  3:241524/1‑148 (MQ=255)                                                                                                                        aCGTCATTGCCCTGGCCCAGCAAGGCCTGCGCAATGCCGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGCCTCGTACAGCCCGTACAGCTCCTGCCCCTTGTGGAAAACCGGGGTTTCCggg                                 >  3:111628/1‑147 (MQ=37)                                                                                                                        aCGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGCCTCGTACAGCCCGTACAGCTCCTGCCCCTTGTGGAAAACCGGGGTTTCCGGGGa                               <  4:57183/149‑1 (MQ=255)                                                                                                                            cATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGCCTCGTACAGCCCg                                                                        <  2:39565/104‑1 (MQ=255)                                                                                                                             aTTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGCCTCGTACAGCCCGTTCAGCTCCTGCCCCTTGTGGAAAACCGGGGTTTCCGGGGAGTTCa                          <  1:82532/149‑1 (MQ=255)                                                                                                                                     ggCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGCCTCGTACAGCCCGTACAGCTCCTGCCCCTTGTGGAAAACCGGGGTTTCCGGGGAGTTCAGGTACTTg                  <  2:379420/149‑1 (MQ=255)                                                                                                                                       ccTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGCCTCGTACAGCCCGTACAGCTCCTGCCCCTTGTGGAAAACCGGGGTTTCCGGGGAGTTCAGGTACTTggg                <  2:334250/149‑1 (MQ=255)                                                                                                                                            cTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGCGCTTGCGCGCCTCGTACAGCCCGTACAGCTCCTGCCCCTTGTGGAAAACCGGGGTTTCCGGGGAGTTCAGGTACTTGGGCTTgt           <  4:241524/149‑1 (MQ=255)                                                                                                                                              gggCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGCCTCGTACAGCCCGTACAGCTCCTGCCCCTTGTGGAAAACCGGGGTTTCCGGGGAGTTCAGGTACTTGGGCTTgtcg         >  2:243328/1‑149 (MQ=255)                                                                                                                                              gggCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGCCTCGTACAGCCCGTACAGCTCCTGCCCCTTGTGGAAAACCGGGGTTTCCGGGGAGTTCAGGTACTTGGGCTTgtcg         >  6:170040/1‑149 (MQ=255)                                                                                                                                               ggCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGCCTCGTACAGCCCGTACAGCTCCTGCCCCTTGTGGAAAACCGGGGTTTCCGGGGAGTTCAGGTACTTGGGCTTgtcgt        >  5:224019/1‑149 (MQ=255)                                                                                                                                                 ccAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGCCTCGTACAGCCCGTACAGCTCCTGCCCCTTGTGGAAAACCGGGGTTTCCGGGGAGTTCAGGTACTTGGGCTTGtcgtcg      >  7:386739/1‑149 (MQ=255)                                                                                                                                                     ggCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGCCTCGTACAGCCCGTACAGCTCCTGCCCCTTGTGGAAAACCGGGGTTTCCGGGGAGTTCAGGTACTTGGGCTTGTCGTCGcc    >  6:32749/1‑147 (MQ=255)                                                                                                                                                     ggCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGCCTCGTACAGCCCGTACAGCTCCTGCCCCTTGTGGAAAACCGGGGTTTCCGGGGAGTTCAGGTACTTGGGCTTGTCGTCGCCGa  <  4:259945/149‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                      GTGCGCGCCAGGCGGCCTTGCGCCCGGCCTGGTCGCCGTCAAAACAGAACAGCACGCTGGGCACCACGCGGAACAGGCGCTTGAGGTGCTCTTCGCTGGTGGCGGTGCCAAGGGTGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGCCTCGTACAGCCCGTACAGCTCCTGCCCCTTGTGGAAAACCGGGGTTTCCGGGGAGTTCAGGTACTTGGGCTTGTCGTCGCCGA  >  NC_002947/474015‑474311

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑