| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,740,474 | C→A | 23.3% | intergenic (+39/+32) | hutF → / ← PP_5037 | formiminoglutamate deiminase/lipocalin family lipoprotein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,740,474 | 0 | C | A | 23.3% | 106.7 / 24.5 | 51 | intergenic (+39/+32) | hutF/PP_5037 | formiminoglutamate deiminase/lipocalin family lipoprotein |
| Reads supporting (aligned to +/- strand): ref base C (16/23); new base A (8/4); total (24/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.87e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGCGATGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAG‑CCCGCGAAGGGTTGCAACGCAACCCCAAAAA‑ACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGT > NC_002947/5740328‑5740619 | cgcgATGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAG‑ccc < 7:347572/148‑1 (MQ=255) aTGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAG‑CCCGCGaa < 5:119309/149‑1 (MQ=255) tgtgATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAG‑CCCGCGAAg < 3:26436/149‑1 (MQ=255) gtgATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGGGCTTG‑CACGCGAAg < 8:139545/148‑1 (MQ=255) tgaggctGAATGGGCATTGGGTGGCGCGCCATGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAG‑CCCGCGAAgg < 8:264309/142‑1 (MQ=255) tgATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAG‑CCCGCGAAgg < 8:285516/148‑1 (MQ=255) tgATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAG‑CCCGCGAAgg < 8:255240/148‑1 (MQ=255) tgATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAG‑CCCGCGAAgg < 3:20407/148‑1 (MQ=255) aTGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAG‑CCCGCGAAGGGtt < 3:278666/149‑1 (MQ=255) tGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAACCCTTCGCGGGCTTG‑CACGCGAAGGGCTg > 4:19698/1‑149 (MQ=38) ggTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAACCCTTCGCGGGCTTG‑CACGCGAAGGGCTg < 3:19698/148‑1 (MQ=38) gTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAACCCTTCGCGGGCTTG‑CACGCGAAGGGCTgc < 5:179484/148‑1 (MQ=38) tGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAG‑CCCGCGAAGGGTTg < 3:333909/142‑1 (MQ=255) tGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAG‑CCCGCGAAGGGTTg > 4:333909/1‑142 (MQ=255) gtggtgCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAG‑CCCGCGAAGGGTTGCAACGCAACCCCaaaa > 5:418315/1‑148 (MQ=255) cgcCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAG‑CCCGCGAAGGGTTGCAACGCAACCCCAAAAA‑ACTCAg < 3:49634/149‑1 (MQ=255) gcCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGCGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAG‑CCCGCTAAGGGTTGAAACGCAACCCCAAAAA‑ACTCAGt < 4:327970/149‑1 (MQ=255) aGGAAGATGAAAGCGGGCGGGCGTTGGCCAAGGTGTTGCGCCAGCTTTTGGGCGGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAG‑CCCGCGAAGGGTTGCAACGCAACCCCAAAAA‑ACTCAGTGCATTTTTACTATTTGCt < 4:313844/149‑1 (MQ=255) aaGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAG‑CCCGCGAAGGGTTGCAACGCAACCCCAAAAA‑ACTCAGTGCATTTTTACTATTTGCTGATCGGacgc > 6:418315‑M2/1‑145 (MQ=255) gcgggcggGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTTGAACCCATACCGGGGAAGCCCCCCCAAGGGTTG‑AACCCAAACCCCAAAA‑AAAAAATGCATTTTTTATATTTTGTTATCGGAcgccc > 2:15111/1‑149 (MQ=255) gggggggcGTTTGCCATGGTGTTGCGCCAGCTTTTGGGCGGAGCGTACCGGGGTGGCGTTGCAGCCCTTCGCGTGCAAG‑CCCGCGATGGGTTGAAACGCAACCCCAAAAA‑ACTCAGTGCATTTTTACTATTTGCTGATCGGacgcccgc > 4:298328‑M2/1‑141 (MQ=255) aGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAG‑CCCGCGAAGGGTTGCAACGCAACCCCAAAAA‑ACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTgg < 3:289787/149‑1 (MQ=255) gcgcCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAG‑CCCGCGAAGGGTTgc < 6:223800/71‑1 (MQ=255) gcgcCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAG‑CCCGCGAAGGGTTgc > 5:223800/1‑71 (MQ=255) gcCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTTCAGCCCTTAGCGTGCAAG‑CCCGCCAAGGGTTGCAACGCCACCCCAAAAA‑AAGAAGTGGATTTTTTATATTTGGTGATAGGACGCCCGCCACATCAAGCGGCGGGTGTAATaac > 6:401799/1‑147 (MQ=255) gcCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTAGCGTGCAAG‑CCCGCGAACGGTTGCAACCCAACCCCAAAAA‑ACTCACTGCAATTTTACTATTTGCGGATCGGACGCCCCCCAGATCAAGCGGAAGGTGTCATAcc > 7:386471/1‑149 (MQ=255) ttttGGGCGGAGCGTACCGGGGTGGCGTTGCAGCCCTTCGCGTGCAAG‑CCCGCTATGGGTTGAAACGCAACCCCAAAAA‑ACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCctgct < 2:361478/149‑1 (MQ=255) ggCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAG‑CCCGCGAAGGGTTGCAACGCAACCCCAAAAA‑ACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCcg < 1:113586/148‑1 (MQ=255) aGCTTACCGGGGTTGCGTTGCAGCCGTGGGCGTGCATC‑CCCGGGAAGGTTTGAAACGCAACCCCAAAAA‑ACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGcc < 5:26406/147‑1 (MQ=255) cGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAG‑CCCGCGAAGGGTTGCAACGCAACCCCAAAAA‑ACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCt < 1:82444/147‑1 (MQ=255) ctttgcAGCCCTTCGCGTGCAAG‑CCCGCTAAGGGTTGCAACGCAACCCCAAAAA‑ACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTtctc < 2:127287/147‑1 (MQ=255) cgttgcAGCCCTTCGCGTGCAAG‑CCCGCGAAGGGTTGCAACGCAACCCCAAAAA‑ACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTtctc < 1:15111/149‑1 (MQ=255) cgttgcAGCCCTTCGCGTGCAAG‑CCCGCGAAGGGTTGCAACGCAACCCCAAAAA‑ACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTtctc < 5:401799/149‑1 (MQ=255) gttgcAGCCCTTGGCGTGCAAG‑CCCGCGAAGGGTTGCAACGCAACCCCAAAAA‑ACTCAGTGCATTTTTTCTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCTCCCTGGACAGCAGGTTCTCa < 8:386471/149‑1 (MQ=255) gCCCTTCGCGTGCAAG‑CCCGCGAAGGGTTGCAACGCAACCCCAAAAA‑ACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTcc > 8:63228/1‑149 (MQ=255) acccTTCGCGGGCTTG‑CACGCGAAGGGCTGCAACGCAACCCCAAAAA‑ACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTcc > 3:264582/2‑149 (MQ=38) acccTTCGCGGGCTTG‑CACGCGAAGGGCTGCAACGCAACCCCAAAAA‑ACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTcc > 5:134336/2‑149 (MQ=38) ccTTCGCGTGCAAG‑CCCGCGAAGGGTTGCAACGCAACCCCAAAAA‑ACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTcc > 3:202247/1‑147 (MQ=255) ccTTCGCGTGCAAG‑CCCGCGAAGGGTTGCAACGCAACCCCAAAAA‑ACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACACCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTcc > 3:359135/1‑147 (MQ=255) ccTTCGCGGGCTTG‑CACGCGAAGGGTTGCAACGCAACCCCAAAAA‑ACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTcc > 8:234595/1‑147 (MQ=255) ccTTCGCGGGCTTG‑CACGCGAAGGGTTGCAACGCAACCCCAAAAA‑ACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCAGGACAGCAGGTTCTCACGCTcc > 2:53415/1‑147 (MQ=39) cTTCGCGGGCTTG‑CACGCGAAGGGTTGCAACGCAACCCCAAAAA‑ACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAg > 5:118184/1‑149 (MQ=255) cTTCGCGGGCTTG‑CACGCGAAGGGCTGCAACGCAACCCCAAAAA‑ACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAg > 2:304780/1‑149 (MQ=39) ttCGCGTGCAAG‑CCCGCGAAGGGTTGCAACGCAACCACAAAAA‑ACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATCCCCCTGCTGCCGCGccc > 2:67209/1‑122 (MQ=255) ttCGCGGGCTTG‑CACGCGAAGGGTTGCAACGCAACCCCAAAAA‑ACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGAcagca < 7:207271/131‑1 (MQ=255) ttCGCGGGCTTG‑CACGCGAAGGGTTGCAACGCAACCCCAAAAA‑ACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGAcagca > 8:207271/1‑131 (MQ=255) gTGCAAG‑CCCGCGAAGGGTTGCAACGCAACCCCAAAAA‑ACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCgg > 6:199676/1‑148 (MQ=255) gTGCAAG‑CCCGCGAAGGGTTGCAACGCAACCCCAAAAA‑ACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCgg > 6:126152/1‑148 (MQ=255) gCAAG‑CCCGCGAAGGGTTGCAACGCAACCCCAAAAA‑ACTCAGTGCATTTTTACTATTTGCTGATCGGacgcccgcccgctttcatcttcctgggcatgccgcccctggcgcaccacccattgcccattcaccatcacatcgcgcacctg < 6:7229‑M2/149‑83 (MQ=255) gCAAG‑CCCGCGAAGGGTTGCAACGCAACCCCAAAAA‑ACTCAGTGCATTTTTACTATTTGCTGATCGGacgcccgcccgctttcatcttcctgggcatgccgcccctgg > 6:43600‑M2/1‑67 (MQ=255) gCAAG‑CCCGCGAAGGGTTGCAACGCAACCCCAAAAA‑ACTCAGTGCATTTTTACTATTTGCTGATCGGacgcccgcccgctttcatcttcctgggcatgccgcccctgg < 5:43600‑M2/108‑42 (MQ=255) g‑CCCGCGAAGGGTTGCAACGCAACCCCAAAAATACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGATCCCAGGCCGGCAgtgt > 1:49069/1‑149 (MQ=255) | CGCGATGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAG‑CCCGCGAAGGGTTGCAACGCAACCCCAAAAA‑ACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGT > NC_002947/5740328‑5740619 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |