Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,748,696 G→T 5.9% N428K (AAC→AAA)  typA ← ribosome associated GTPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,748,6960GT 5.9% 212.1 / 3.2 68N428K (AAC→AAA) typAribosome associated GTPase
Reads supporting (aligned to +/- strand):  ref base G (35/29);  new base T (2/2);  total (37/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.88e-01

CGACTTCATGGTGTCGTAGCGATCGAAGATCGAGGTCAGGATGCCTGCACCGTTGGTCAGGGTCAGGAACTGGTTACGGAAACCGATCAGACCACGGGCTGGAATGTTGTACTCCAGACGAACACGGCCCTTGCCATCCGGAACCATGTTGGTCAGGTCGCCTTTACGCAGACCCATCTCTTCCATGACCTTGCCCTGGGATTCTTCAGGGATGTCGATGGTGACGTTCTCGAACGGCTCCTGCTTCACGCCGTCGACTTCGCGGATGATCACTTCAGGACGGCC  >  NC_002947/5748549‑5748833
                                                                                                                                                   |                                                                                                                                         
cGACTTCATGGTGTCGTAGCGATCGAAGATCGAGGTCAGGATGCCTGCACCGTTGGTCAGGGTCAGGAACTGGTTACGGAAACCGATCAGACCACGGGCTGGAATGTTGTACTCCAGACGAACACGGCCCTTGCCATCCGGAACCATGt                                                                                                                                          <  4:295709/149‑1 (MQ=255)
cGACTTCATGGTGTCGTAGCGATCGAAGATCGAGGTCAGGATGCCTGCACCGTTGGTCAGGGTCAGGAACTGGTTACGGAAACCGATCAGACCACGGGCTGGAATGTTGTACTCCAGACGAACACGGCCCTTGCCATCCGGAACCATGt                                                                                                                                          <  2:319141/149‑1 (MQ=255)
cGACTTCATGGTGTCGTAGCGATCGAAGATCGAGGTCAGGATGCCTGCACCGTTGGTCAGGGTCAGGAACTGGTTACGGAAACCGATCAGACCACGGGCTGGAATGTTGTACTCCAGACGAACACGGCCCTTGCCATCCGGAACCATGt                                                                                                                                          >  3:368328/1‑149 (MQ=255)
cGACTTCATGGTGTCGTAGCGATCGAAGATCGAGGTCAGGATGCCTGCACCGTTGGTCAGGGTCAGGAACTGGTTACGGAAACCGATCAGACCACGGGCTGGAATGTTGTACTCCAGACGAACACGGCCCTTGCCATCCGGAACCATGt                                                                                                                                          <  3:244152/149‑1 (MQ=255)
 gACTTCATGGTGTCGTAGCGATCGAAGATCGAGGTCAGGATGCCTGCACCGTTGGTCAGGGTCAGGAACTGGTTACGGAAACCGATCAGACCACGGGCTGCAATGTTGTACTCCAGACGAACACGGCCCTTGCCATCCGGAACCATGtt                                                                                                                                         >  5:244114/1‑149 (MQ=255)
  aCTTCATGGTGTCGTAGCGATCGAAGATCGAGGTCAGGATGCCTGCACCGTTGGTCAGGGTCAGGAACTGGTTACGGAAACCGATCAGACCACGGGCTGGAATGTTGTACTCCAGACGAACACGGCCCTTGCCATCCGGAACCATGTTg                                                                                                                                        <  5:312459/149‑1 (MQ=255)
    ttCATGGTGTCGTAGCGATCGAAGATCGAGGTCAGGATGCCTGCACCGTTGGTCAGGGTCAGGAACTGGTTACGGAAACCGATCAGACCACGGGCTGGAATGTTGTACTCCAGACGAACACGGCCCTTGCCATCCGGAACCATGTTGGt                                                                                                                                      >  6:79326/1‑149 (MQ=255)
          gtgtCGTAGCGATCGAAGATCGAGGTCAGGATGCCTGCACCGTTGGTCAGGGTCAGGAACTGGTTACGGAAACCGATCAGACCACGGGCTGGAATGTTGTACTCCAGACGAACACGGCCCTTGCCATCCGGAACCATGTTGGTCAGGTc                                                                                                                                <  5:407530/149‑1 (MQ=255)
            gtCGTAGCGATCGAAGATCGAGGTCAGGATGCCTGCACCGTTGGTCAGGGTCAGGAACTGGTTACGGAAACCGATCAGACCACGGGCTGGAATGTTGTACTCCAGACGAACACGGCCCTTGCCATCCGGAACCATGTTGGTCAGGTCGc                                                                                                                              <  2:370056/149‑1 (MQ=255)
                    gATCGAAGATCGAGGTCAGGATGCCTGCACCGTTGGTCAGGGTCAGGAACTGGTTACGGAAACCGATCAGACCACGGGGTGGAATGTTGTACTCCAGACGAACACGGCCCTTGCGATCCGGAACCATGTTGGTCAGGTCGCCTTTACg                                                                                                                       >  7:152614/1‑148 (MQ=255)
                     aTCGAAGATCGAGGTCAGGATGCCTGCACCGTTGGTCAGGGTCAGGAACTGGTTACGGAAACCGATCAGACCACGGGCTGGAATGTTGTACTCCAGACGAACACGGACCTTGCCATCCGGAACCATGTTGGTCAGGTCGCCTTTACg                                                                                                                       >  8:36090/1‑147 (MQ=255)
                      tCGAAGATCGAGGTCAGGATGCCTGCACCGTTGGTCAGGGTCAGGAACTGGTTACGGAAACCGATCAGACCACGGGCTGGAATGTTGTACTCCAGACGAACACGGCCCTTGCCATCCGGAACCATGTTGGTCAGGTCGCCTTTACGCAg                                                                                                                    >  7:201576/1‑149 (MQ=255)
                                  gTCAGGATGCCTGCACCGTTGGTCAGGGTCAGGAACTGGTTACGGAAACCGATCAGACCACGGGCTGGAATGTTGTACTCCAGACGAACACGGCCCTTGCCATCCGGAACCATGTTGGTCAGGTCGCCTTTACGCAGACCCATCTCTTc                                                                                                        <  4:399498/149‑1 (MQ=255)
                                       gATGCCTGCACCGTTGGTCAGGGTCAGGAACTGGTTACGGAAACCGATCAGACCACGGGCTGGAATGTTGTACTCCAGACGAACACGGCCCTTGCCATCCGGAACCATTTTGGTc                                                                                                                                     >  3:139990/1‑115 (MQ=255)
                                       gATGCCTGCACCGTTGGTCAGGGTCAGGAACTGGTTACGGAAACCGATCAGACCACGGGCTGGAATGTTGTACTCCAGACGAACACGGCCCTTGCCATCCGGAACCATTTTGGTc                                                                                                                                     >  3:145868/1‑115 (MQ=255)
                                       gATGCCTGCACCGTTGGTCAGGGTCAGGAACTGGTTACGGAAACCGATCAGACCACGGGCTGGAATGTTGTACTCCAGACGAACACGGCCCTTGCCATCCGGAACCATTTTGGTc                                                                                                                                     <  4:145868/115‑1 (MQ=255)
                                       gATGCCTGCACCGTTGGTCAGGGTCAGGAACTGGTTACGGAAACCGATCAGACCACGGGCTGGAATGTTGTACTCCAGACGAACACGGCCCTTGCCATCCGGAACCATTTTGGTc                                                                                                                                     <  4:139990/115‑1 (MQ=255)
                                           ccTGCACCGTTGGTCAGGCTCAGGAACTGGTTACGGAAACCGATCAGACCACGGGCTGGAATGTTGTACTCCAGACGAACACGGCACTTGCCATCCGGAACCATGTTTGt                                                                                                                                      >  3:26984/1‑110 (MQ=255)
                                             tGCACCGTTGGTCAGGGTCAGGAACTGGTTACGGAAACCGATCAGACCACGGGCTGGAATGTTGTACTCCAGACGAACACGGCCCTTGCCATCCGGAACCATGTTGGTCAGGTCGCCTTTACGCAGACCCATCTCTTCCATGACCTTGc                                                                                             >  1:64325/1‑149 (MQ=255)
                                               cACCGTTGGTCAGGGTCAGGAACTTGTTACGGAAACCGATCAGACCACGGGCTGGAATGTTGTACTCCAGACGAACACGGCCCTTGCCATCCGGAACCATGTTGGTCAGGTCGCCTTTACGCAGACCCATCTCTTCCATGACCTTGc                                                                                             <  1:31661/147‑1 (MQ=255)
                                               cACCGTTGGTCAGGGTCAGGAACTGGTTACGGAAACCGATCAGACCACGGGCTGGAATGTTGTACTCCAGACGAACACGGCCCTTGCCATCCGGAACCATGTTGGTAAGGTCGCCTTTACGCATACGCATCTCTTCCATGACCTTGcca                                                                                           >  5:102974/1‑148 (MQ=255)
                                                  cGTTGGTCAGGGTCAGGAACTGGTTACGGAAACCGATCAGACCACGGGCTGGAATGTTGTACTCCAGACGAACACGGCCCTTGCCATCCGGAACCATGTTGGTCAGGTCGCCTTTACGCAGACCCATCTCTTCCATGACCTTGCCCTgg                                                                                        <  2:64325/149‑1 (MQ=255)
                                                    ttGGTCAGGGTCAGGAACTGGTTACGGAAACCGATCAGACCACGGGCTGGAATGTTGTACTCCAGACGAACACGGCCCTTGCCATCCGGAACCATGTTGGTCAGGTCGCCTTTACGCAGACCCATCTCTTCCATGACCTTGCCCTGGGa                                                                                      >  2:408133/1‑149 (MQ=255)
                                                       gtcagggtcaggAACTGGTTACGGAAACCGATCAGACCACGGGCTGGAATGTTGTACTCCAGACGAACACGGCCCTTGCCATCCGGAACCATGTTGGTCAGGTCGCCTTTACGCAGACCCATCTCTTCCATGACCTTGCCCTGGGAttc                                                                                   >  3:21860/1‑149 (MQ=255)
                                                        tcagggtcaggAACTGGTTACGGAAACCGATCAGACCACGGGCTGGAATGTTGTACTCCAGACGAACACGGCCCTTGCCATCCGGAACCATGTTGGTCAGGTCGCCTTTACGCAGACCCATCTCTTCCATGACCTTGCCCTGGGAttct                                                                                  >  6:229560/1‑149 (MQ=255)
                                                          agggtcaggAACTGGTTACGGAAACCGATCAGACCACGGGCTGGAATGTTGTACTCCAGACGAACACGGCCCTTGCCATCCGGAACCATGTTGGTCAGGTCGCCTTTACGCAGACCCATCTCTTCCATGACCTTGCCCTggg                                                                                       <  3:81675/142‑1 (MQ=255)
                                                          agggtcaggAACTGGTTACGGAAACCGATCAGACCACGGGCTGGAATGTTGTACTCCAGACGAACACGGCCCTTGCCATCCGGAACCATGTTGGTCAGGTCGCCTTTACGCAGACCCATCTCTTCCATGACCTTGCCCTggg                                                                                       >  4:81675/1‑142 (MQ=255)
                                                           gggtcaggAACTGGTTACGGAAACCGATCAGACCACGGGCTGGAATGTTGTACTCCAGACGAACACGGCCCTTGCCATCCGGAACCATGTTGGTCAGGTCGCCTTTACGCAGACCCATCTCTTCCATGACCTTGCCCTGGGAttctt                                                                                 >  6:159028/1‑147 (MQ=255)
                                                             gtcagcAACTCGATACGGAAACCGATCACACCACTGGCTGGAATGTGGTACTCCAGACGATCCCCGCCCTTGCCATCCGGAACCATGGTGCTCAGGTCGCCTTTACGCAGACCCATCTCTTCCATGACCTTGCCCTGGGATTCATCAgg                                                                             <  8:152614/149‑1 (MQ=255)
                                                              tcaggAACTGGTTACGGAAACCGATCAGACCACGGGCTGGAATGTTGTACTCCAGACGAACACGGCCCTTGCCATCCGGAACCATGTTGGTCAGGTCGCCTTTACGCAGACCCATCTCTTCCATGACCTTGCCCTGGGATTCTTCAggg                                                                            <  5:229560/149‑1 (MQ=255)
                                                              tcaggAACTGGTTACGGAAACCGATCAGACCACGGGCTGGAATGTTGTACTCCAGACGAACACGGCCCTTGCCATCCGGAACCATGTTGGTCAGGTCGCCTTTACGCAGACCCATCTCTTCCATGACCTTGCCCTGGGATTCTTCAgg                                                                             >  3:44661/1‑148 (MQ=255)
                                                              tcaggAACTGGTTACGGAAACCGATCAGACAACGCTATGGAATGTTGTACTCCAGACGAACACGGCCCTTGCCATACGGAACCATGATGGTCAGGTCGCAGTTACGCAGACCCATCTCATCAATGACATTGCACTGGGATTCTTCAgg                                                                             >  8:29353/1‑148 (MQ=255)
                                                                  gAACTGGTTACGGAAACCGATCAGACCACGGGCTGGAATGTTGTACTCCAGACGAACACGGCCCTTGCCATCCGGAACCATGTTGGTCAGGTCGCCTTTACGCAGACCCATCTCTTCCATGACCTTGCCCTGGGATTCTTCAGGGATg                                                                         >  4:127164/1‑148 (MQ=255)
                                                                  gAACTGGTTACGGAAACCGATCAGACCACGGGCTGGAATGTTGTACTCCAGACGAACACGGCCCTTGCCATCCGGAACCATGTTGGTCAGGTCGCCTTTACGCAGACCCATCTCTTCCATGACCTTGCCCTGGGATTCTTCAGGGATGt                                                                        >  3:173148/1‑149 (MQ=255)
                                                                  gAACTGGTTACGGAAACCGATCAGACCACGGGCTGGAATGTTGTACTCCAGACGAACACGGCCCTTGCCATCCGGAACCATGTTGGTCAGGTCGCCTTTACGCAGACCCATCTCTTCCATGACCTTGCCCTGGGATTCTTCAGGGATGt                                                                        <  3:127164/149‑1 (MQ=255)
                                                                      tGGTTACGGAAACCGATCAGACCACGGGCTGGAATGTTGTACTCCAGACGAACACGGCCCTTGCCATCCGGAACCATGTTGGTCAGGTCGCCTTTACGCAGACCCATCTCTTCCATGACCTTGCCCTGGGATTCTTCAGGGATGTCGAt                                                                    >  8:111046/1‑149 (MQ=255)
                                                                        gTTACGGAAACCGATCAGACCACGGGCTGTAATGTTGTACTCCAGACGAACACGGCCCTTGCCATCCGGAACCATGTTGGTCAGGTCGCCTTTACGCAGACCCATCTCTTCCATGACCTTGCCCTGGGATTCTTCAGGGATGTCGATg                                                                   <  6:244114/148‑1 (MQ=255)
                                                                                 aCCGATCAGACCACGGGCTGGAATGTTGTACTCCAGACGAACACGGCCCTTGCCATCCGGAACCATGTTGGTCAGGTCGCCTTTACGCAGACCCATCTCTTCCATGACCTTGCCCTGGGATTCTTCAGGGATGTCGATGGTGACGTtct                                                         >  6:18025/1‑149 (MQ=255)
                                                                                 aCCGATCAGACCACGGGCTGGAATGTTGTACTCCAGACGAACACGGCCCTTGCCATCCGGAACCATGTTGGTCAGGTCGCCTTTACGCAGACCCATCTCTTCCATGACCTTGCCCTGGGATTCTTCAGGGATGTCGATGGTGACGTtct                                                         <  8:587/149‑1 (MQ=255)
                                                                                       cAGACCACGGGCTGGAATGTTGTACTCCAGACGAACACGGCCCTTGCCATCCGGAACCATGTTGGTCAGGTCGCCTTTACGCAGACCCATCTCTTCCATGACCTTGCCCTGGGATTCTTCAGGGATGTCGATGGTGACGTTCTCGAAc                                                    <  5:159028/148‑1 (MQ=255)
                                                                                         gACCACGGGCTGGAATGTTGTACTCCAGACGAACACGGCCCTTGCCATCCGGAACCATGTTGGTCAGGTCGCCTTTACGCAGACCCATCTCTTCCATGACCTTGCCCTGGGATTCTTCAGGGATGTCGAt                                                                    <  7:274434/130‑1 (MQ=255)
                                                                                         gACCACGGGCTGGAATGTTGTACTCCAGACGAACACGGCCCTTGCCATCCGGAACCATGTTGGTCAGGTCGCCTTTACGCAGACCCATCTCTTCCATGACCTTGCCCTGGGATTCTTCAGGGATGTCGAt                                                                    >  8:274434/1‑130 (MQ=255)
                                                                                            cACGGGCTGGAATGTTGTACTCCAGACGAACACGGCCCTTGCCATCCGGAACCATGTTGGTCAGGTCGCCTTTACGCAGACCCAtctc                                                                                                           <  3:339956/88‑1 (MQ=255)
                                                                                            cACGGGCTGGAATGTTGTACTCCAGACGAACACGGCCCTTGCCATCCGGAACCATGTTGGTCAGGTCGCCTTTACGCAGACCCAtctc                                                                                                           >  4:339956/1‑88 (MQ=255)
                                                                                            cACGGGCTGGAATGTTGTACTCCAGACGAACACGGCCCTTGCCATCCGGAACCATGTTGGTCAGGTCGCCTTTACGCAGACCCATCTCTTCCATGACCTTGCCCTGGGATTCTTCAGGGATGTCGATGGTGACGTTCTCGAACGGCTcc                                              >  8:111167/1‑149 (MQ=255)
                                                                                             aCGGGCTGGAATGTTGTACTCCAGACGAACACGGCCCTTGCCATCCGGAACCATGTTGGTCAGGTCGCCTTTACGCAGACCCATCTCTTCCATGACCTTGCCCTGGGATTCTTCAGGGATGTCGATGGTGACGTTCTCGAACGGCTCCt                                             >  7:46496/1‑149 (MQ=255)
                                                                                               gggCTGGAATGTTGTACTCCAGACGAACACGGCCCTTGCCATCCGGAACCATGTTGGTCAGGTCGCGTTTACGCAGACCCATCTCTTCCATGACCTTGCCCTGGGATTCTTCAGGGATGTCGATGGTGACGTTCTCGAACGGCTCCTGc                                           >  5:295226/1‑149 (MQ=255)
                                                                                                   tGGAATGTTGTACTCCAGACGAACACGGCCCTTGCCATCCGGAACCATGTTGGTCAGGTCGCCTTTACGCAGACCCATCTCTTCCATGACCTTGCCCTGGGATTCTTCAGGGATGTCGATGGTGACGTTCTCGAACGGCTCCTGCTTCa                                       >  7:96633/1‑149 (MQ=255)
                                                                                                     gAATGTTGTACTCCAGACGAACACGGCCCTTGCCATCCGGAACCATGTTGGTCAGGTCGCCTTTACGCAGACCCATCTCTTCCATGACCTTGCCCTGGGATTCTTGAGGGATGTCGATGGTGACGTTCTCGAACGGCTCCTGCTTCACg                                     >  3:287471/1‑149 (MQ=255)
                                                                                                     gAATGTTGTACTCCAGACGAACACGGCCCTTGCCATCCGGAACCATGTTGGTCAGGTCGCCTTTACGCAGACCCATCTCTTCCATGACCTTGCCCTGGGATTCTTCAGGGATGTCGATGGTGACGTTCTCGAACGGCTCCTGCTTCAc                                      <  1:408937/148‑1 (MQ=255)
                                                                                                     gAATGTTGTACTCCAGACGAACACGGCCCTTGCCATCCGGAACCATGTTGGTCAGGTCGCCTTTACGCAGACCCATCTCTTCCATGACCTTGCCCTGGGATTCTTCAGGGATGTCGATGGTGACGTTCTCGAACGGCTCCTGCTTCACg                                     <  4:368328/149‑1 (MQ=255)
                                                                                                     gAATGTTGTACTCCAGACGAACACGGCCCTTGCCATCCGGAACCATGTTGGTCAGGTCGCCTTTACGAAGACCCATCTCTTCCATGACCTTGCCCTGGGATTCTTCAGGGATGTCGATGGTGACGTTCTCGAACGGCTCCTGCTTCACg                                     >  5:202597/1‑149 (MQ=255)
                                                                                                             tACTCCAGACGAACACGGCCCTTGCCATCCGGAACCATGTTGGTCAGGTCGCCTTTACGCAGACCCATCTCTTCCATGACCTTGCCCTGGGATTCTTCAGGGATGTCGATGGTGACGTTCTCGAACGGCTCCTGCTTCACGCCGTCGAc                             >  1:260182/1‑149 (MQ=255)
                                                                                                                   aGACGAACACGGCCCTTGCCATCCGGAACCATGTTGGTCAGGTCGCCTTTACGCAGACCCATCTCTTCCATGACCTTGCCCTGGGATTCTTCAGGGATGTCGATGGTGACGTTCTCGAACGGCTCCTGCTTCACGCCGTCGACTTcgcg                       <  1:408133/149‑1 (MQ=255)
                                                                                                                   aGACGAACACGGCCCTTGCCATCCGGAACCATGTTGGTCAGGTCGCCTTTACGCAGACCCATCTCTTCCATGACCTTGCCCTGGGATTCTTCAGGGATGTCGATGGTGACGTTCTCGAACGGCTCCTGCTTCACGCCGTCGACTTcgcg                       >  5:25250/1‑149 (MQ=255)
                                                                                                                    gACGAACACGGCCCTTGCCATCAGGAACCATGTTGGTCAGGTCGCCTTTACGCAGACCCATCTCTTCCATGACCTTGCCCTGGGATTCTTCAGGGATGTCGATGGTGACGTTCTCGAACGGCTCCTGCTTCACGCCGTCGACTTCGCgg                      <  4:21860/149‑1 (MQ=255)
                                                                                                                     aCGAACACGGCCCTTGCCATCCGGAACCATGTTGGTCAGGTCGCCTTTACGCAGACCCATCTCTTCCATGACCTTGCCCTGGGATTCTTCAGGGATGTCGATGGTGACGTTCTCGAACGGCTCCTGCTTCACGCCGTCGACTTCGCGga                     <  6:25250/149‑1 (MQ=255)
                                                                                                                      cGAACACGGCCCTTGCCATCCGGAACCATGTTGGTCAGGTCGCCTTTACGCAGACCCATCTCTTCCATGACCTTGCCCTGGGATTCTTCAGGGATGTCGATGGTGACGTTCTCGAACGGCTCCTGCTTCACGCCGTCGACTTCGCGgat                    <  6:202597/149‑1 (MQ=255)
                                                                                                                      cGAACACGGCCCTTGCCATCCGGAACCATGTTGGTCAGGTCGCCTTTACGCAGACCCATCTCTTCCATGACCTTGCCCTGGGATTCTTCAGGGATGTCGATGGTGACGTTCTCGAACGGCTCCTGCTTCACGCCGTCGACTTCGCGgat                    <  4:44661/149‑1 (MQ=255)
                                                                                                                       gAACACGGCCCTTGCCATCCGGAACCATGTTGGTCAGGTCGCCTTTACGCAGACCCATCTCTTCCATGACCTTGCCCTGGGATTCTTCAGGGATGTCGATGGTGATGTTCTCGAACGGCTCCTGCTTCACGCCGTCGACTTCGCGgat                    >  1:173510/1‑148 (MQ=255)
                                                                                                                         acacGGCCCTTGCCATCCGGAACCATGTTGGTCAGGTCGCCTTTACGCAGACCCATCTCTTCCATGACCTTGCCCTGGGATTCTTCAGGGATGTCGATGGTGACGTTCTCGAACGGCTCCTGCTTCACGCCGTCGACTTCGCGgatga                  >  2:42840/1‑148 (MQ=255)
                                                                                                                           acGGCCCTTGCCATCCGGAACCATGTTGGTCAGGTCGCCTTTACGCAGACCCATCTCTTCCATGACCTTGCCCTGGGATTCTTCAGGGATGTCGATGGTGACGTTCTCGAACGGCTCCTGCTTCACGCCGTCGACTTCGCGGATGATc                >  3:393056/1‑148 (MQ=255)
                                                                                                                               cccTTGCCATCCGGAACCATGTTGGTCAGGTCGCCTTTACGCAGACCCATCTCTTCCATGACCTTGCCCTGGGATTCTTCAGGGATGTCGATGGTGACGTTCTCGAACGGCTCCTGCTTCACGCCGTCGACTTCGCGGATGATCACtt            >  7:273579/1‑148 (MQ=255)
                                                                                                                                  ttGCCATCCGGAACCATGTTGGTCAGGTCGCCTTTACGCAGACCCATCTCTTCCATGACCTTGCCCGGGGATTCTTCAGGGATGTCGATGGTGACGTTCTCGAACGGCTCCTGCTTCACGCCGTCGACTTCGCGGATGATCACTTCAg         >  4:110211/1‑148 (MQ=255)
                                                                                                                                    gCCATCCGGAACCATGTTGGTCAGGTCGCCTTTACGCAGACCCATCTCTTCCATTACCTTGCCCTGGTATTCTTCAGGGATGTCGATGGTGACGTTCTCGAACGGCTCCTGCTTCACGCCGTCGACTTCGCGGATGATCACTTCAGGa       <  4:256570/148‑1 (MQ=255)
                                                                                                                                    gCCATCCGGAACCATGTTGGTCAGGTCGCCTTTACGCAGACCCATCTCTTCCATGACCTTGCCCTGGGATTCTTCAGGGATGTCGATGGTGACGTTCTCGAACGGCTCCTGCTTCACGCCGTCGACTTCGCGGATGATCACTTCAgg        <  7:111167/147‑1 (MQ=255)
                                                                                                                                      cATCCGGAACCATGTTGGTCAGGTCGCCTTTACGCAGACCCATCTCTTCCATGACCTTGCCCTGGGATTCTTCAGGGATGTCGATGGTGGCGTTCTCGAACGGCTCCTGCTTCACGCCGTCGACTTCGCGGATGATCACTTCAGGACgg    <  7:29353/149‑1 (MQ=255)
                                                                                                                                        tCCGGAACCATGTTGGTCAGGTCGCCTTTACGCAGACCCATCTCTTCCATGACCTTGCCCTGGGATTCTTCAGGGATGTCGATGGTGACGTTCTCGAACGGCTCCTGCTTCACGCCGTCGACTTCGCGGATGATCACTTCAGGACGGcc  <  4:173148/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                         
CGACTTCATGGTGTCGTAGCGATCGAAGATCGAGGTCAGGATGCCTGCACCGTTGGTCAGGGTCAGGAACTGGTTACGGAAACCGATCAGACCACGGGCTGGAATGTTGTACTCCAGACGAACACGGCCCTTGCCATCCGGAACCATGTTGGTCAGGTCGCCTTTACGCAGACCCATCTCTTCCATGACCTTGCCCTGGGATTCTTCAGGGATGTCGATGGTGACGTTCTCGAACGGCTCCTGCTTCACGCCGTCGACTTCGCGGATGATCACTTCAGGACGGCC  >  NC_002947/5748549‑5748833

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑