Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	483,095	C→T	6.6%	Q448Q (CAG→CAA)	yeaG ←	protein kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	483,095	0	C	T	6.6%	221.2 / 2.2	75	Q448Q (CAG→CAA)	yeaG	protein kinase
Reads supporting (aligned to +/- strand):  ref base C (35/35);  new base T (2/3);  total (37/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTCTGGCCGTATTCGCTGTAGGACTCCAGGTAGGCAGTCTGGATTTCCTTGCCGATAAACTCGATGTAGCGAGGTGCCAGGTATTCCTTCAGGTAGCGCAGATAGCGTTCGCGCACTTCAGCCGGGAATTGCTCCTGTTCGATCTGCTGTTCGAGGACATACAGCAGGTGCACCGGGTTGGCGGCAATTTCGTGTGGGTCGAAGTTGAACACCTTGGACAGGATCTTGAAGGCGAAGCGAGTCGACAGGCCGTTCATGCCTTCGTCGACACCCGCAGAGTCCCGGTATTCCTGGA  >  NC_002947/482949‑483243                                                                                                                                                   |                                                                                                                                                     ttCTGGCCGTATTCGCTGTAGGACTCCAGGTAGGCAGTCTGGATTTCCTTGCCGATAAACTCGATGTAGCGAGGTGCCAGGTATTCCTTCAGGTAGCGCAGATAGCGTTCGCGCACTTCAGCCGGGAATTGCTCCTGTTCGATctgct                                                                                                                                                     <  3:166833/148‑1 (MQ=255) ttCTGGCCGTATTCGCTGTAGGACTCCAGGTAGGCAGTCTGGATTTCCTTGCCGATAAACTCGATGTAGCGAGGTGCCAGGTATTCCTTCAGGTAGCGCAGATAGCGTTCGCGCACTTCAGCCGGGAATTGCTCCTGTTCGATctgc                                                                                                                                                      <  8:191986/147‑1 (MQ=255) ttCTGGCCGTATTCGCTGTAGGACTCCAGGTAGGCAGTCTGGATTTCCTTGCCGATAAACTCGATGTAGCGAGGTGCCAGGTATTCCTTCAGGTAGCGCAGATAGCGTTCGCGCACTTCAGCCGGGAATTGCTCCTGTTCGATctgc                                                                                                                                                      >  7:191986/1‑147 (MQ=255)   cTGGCCGTATTCGCTGTAGGACTCCAGGTAGGCAGTCTGGATTTCCTTGCCGATAAACTCGATGTAGCGAGGTGCCAGGTATTCCTTCAGGTAGCGCAGATAGCGTTCGCGCACTTCAGCCGGGAATTGCTCCTGTTCGATCTGCTGtt                                                                                                                                                  >  2:327236/1‑149 (MQ=255)      gCCGTATTCGCTGTAGGACTCCAGGTAGGCAGTCTGGATTTCCTTGCCGATAAACTCGATGTAGCGAGGTGCCAGGTATTCCTTCAGGTAGCGCAGATAGCGTTCGCGCACTTCAGCCGGGAATTGCTCCTGTTCGATCTGCTGTTCGa                                                                                                                                               <  2:182545/149‑1 (MQ=255)       ccGTATTCGCTGTAGGACTCCAGGTAGGCAGTCTGGATTTCCTTGCCGATAAACTCGATGTAGCGAGGTGCCAGGTATTCCTTCAGGTAGCGCAGATAGCGTTCGCGCACTTCAGCCGGGAATTGCTCCTGTTCGATCTGCTGTTCGAg                                                                                                                                              <  4:343542/149‑1 (MQ=255)         gTATTCGCTGTAGGACTCCAGGTAGGCAGTCTGGATTTCCTTGCCGATAAACTCGATGTAGCGAGGTGCCAGGTATTCCTTCAGGTAGCGCAGATAGCGTTCGCGCACTTCAGCCGGGAATTGCTCCTGTTCGATCTGCTGTTCGAGGa                                                                                                                                            >  3:265980/1‑149 (MQ=255)         gTATTCGCTGTAGGACTCCAGGTAGGCAGTCTGGATTTCCTTGCCGATAAACTCGATGTAGCGAGGTGCCAGGTATTCCTTCAGGTAGCGCAGATAGCGTTCGCGCACTTCAGACGGGAATTGCTCCTGTTCGATCTGTTGTTctacga                                                                                                                                            >  4:115092/1‑144 (MQ=255)         gTATTCGCTGTAGGACTCCAGGTAGGCAGTCTGGATTTCCTTGCCGATAAACTCGATGTAGCGAGGTGCCAGGTATTACTTCAGGTAGCGCAGATAGCGTTCGCGCACTTCAGCCGGGAATTGCTCCTGTTCGATCTGTTTTTCGAGGa                                                                                                                                            >  4:109536/1‑149 (MQ=255)            ttCGCTGTAGGACTGCAGGTAGGCAGTCTGGATTTCCTTGCCGATAAACTCGATGTAGCGAGGTGCCAGGTATTCCTTCAGGTAGCGCAGATAGCGTTCGCGCACTTCAGCCGGGAATTGCTCCTGTTCGATCTGCTGTTCGAGGACa                                                                                                                                          <  1:321772/148‑1 (MQ=255)                cTGTAGGACTCCAGGTAGGCAGTCTGGATTTCCTTGCCGATAAACTCGATGTAGCGAGGTGCCAGGTATTCCTTCAGGTAGCGCAGATAGCGTTCGCGCACTTCAGCCGGGAATTGCTCCTGTTCGATCTGCTGTTCGAGGACATAcag                                                                                                                                     <  4:265980/149‑1 (MQ=255)                cTGTAGGACTCCAGGTAGGCAGTCTGGATTTCCTTGCCGATAAACTCGATGTAGCGAGGTGCCAGGTATTCCTTCAGGTAGCGCAGATAGCGTTCGCGCACTTCAGCCGGGAATTGCTCCTGTTCGATCTGCTGTTCGAGGACATAcag                                                                                                                                     >  3:357158/1‑149 (MQ=255)                cTGTAGGACTCCAGGTAGGCAGTCTGGAGTTCCTTGCCGATATAGTCGTTGTAGCGAGGTGCCAGGTATTCCTTCAGGTAGCGCAGATAGCGTTCGCGCACTTCAGCCGGGAATTGATCGTGTTCGATGTGCTGTTCGAGGACATAcag                                                                                                                                     <  6:346515/149‑1 (MQ=255)                 tGTAGGACTCCAGGTAGGCAGTCTGGATTTCCTTGCCGATAAACTCGATGTAGCGAGGTGCCAGGTATTCCTTCAGGTAGCGCAGATAGCGTTCGCGCACTTCCGCCGGGAATTGCTCCTGTTCGATCTGCTGTTCGAGGACATAcagc                                                                                                                                    >  4:140478/1‑149 (MQ=255)                 tGTAGGACTCCAGGTAGGCAGTCTGGATTTCCTTGCCGATAAACTCGATGTAGCGAGGTGCCAGGTATTCCTTCAGGTAGCGCAGATAGCGTTCGCGCACTTCAGCCGGGAATTGCTCCTGTTCGATCTGCTGTTCGAGGACATAcag                                                                                                                                     >  4:246462/1‑148 (MQ=255)                    aGGACTCCAGGTAGGCAGTCTGGATTTCCTTGCCGATAAACTCGATGTAGCGAGGTGCCAGGTATTCCTTCAGGTAGCGCAGATAGCGTTCGCGCACTTCAGCCGGGAATTGCTCCTGTTCGATCTGCTGTTCGAGGACATACAGCAgg                                                                                                                                 >  6:252844/1‑149 (MQ=255)                         tCCAGGTAGGCAGTCTGGATTTCCTTGCCGATAAACTCGATGTAGCGAGGTGCCAGGTATTCCTTCAGGTAGCGCAGATAGCGTTCGCGCACTTCAGCCGGGAATTGCTCCTGTTCGATCTGCTGTTCGAGGACATACAGCAGGTGCAc                                                                                                                            >  2:399640/1‑149 (MQ=255)                           cAGGTAGGCAGTCTTGATTTCCTTGCCGATAAACTCGATGTAGAGAGTTGGCTGGTATTGCTTCAGGTAGCGCAGATAGAGTTCACGCACTTCAGCCGGGAATTGCTCCTTTTCGATCTGTTTTTCGAGGAAATAGAGCAGGTGCAcag                                                                                                                          <  2:220499/149‑3 (MQ=255)                           cAGGTAGGCAGTCTGGATTTCCTTGCCGATAAACTCGATGTAGCGAGGTGCCAGGTATTCCTTCAGGTAGCGCAGATAGCGTTCGCGCACTTCAGCCGGGAATTGCTCCTGTTCGATctgct                                                                                                                                                     >  5:300982/1‑122 (MQ=255)                           cAGGTAGGCAGTCTGGATTTCCTTGCCGATAAACTCGATGTAGCGAGGTGCCAGGTATTCCTTCAGGTAGCGCAGATAGCGTTCGCGCACTTCAGCCGGGAATTGCTCCTGTTCGATctgct                                                                                                                                                     <  6:300982/122‑1 (MQ=255)                            aGGTAGGCAGTCTGGACTTCCTTGCCGATAAACTCGATGTAGCGAGGTGCCAGGTATTCCTTCAGGTAGCGCAGATAGCGTTCGCGCACTTCAGCCGGGAATTGCTCCTGTTCGATCTGCTGTTCGAGGACATACAGCAGGTGCACCgg                                                                                                                         <  5:101461/149‑1 (MQ=255)                                   cAGTCTGGATTTCCTTGCCGATAAACTCGATGTAGCGAGGTGCCAGGTATTCCTTCAGGTAGCGCAGATAGCGTTCGCGCACTTCAGCCGGGAATTGCTCCTGTTCGATCTGCTGTTCGAGGACATACAGCAGGTGCACCGGGTTggcg                                                                                                                  >  8:8705/1‑149 (MQ=255)                                    aGTCTGGATTTCCTTGCCGATAAACTCGATGTAGCGAGGTGCCAGGTATTCCTTCAGGTAGCGCAGATAGCGTTCGCGCACTTCAGCCGGGAATTGCTCCTGTTCGATCTGCTGTTCGAGGACATACAGCAGGt                                                                                                                                <  4:361669/134‑1 (MQ=255)                                    aGTCTGGATTTCCTTGCCGATAAACTCGATGTAGCGAGGTGCCAGGTATTCCTTCAGGTAGCGCAGATAGCGTTCGCGCACTTCAGCCGGGAATTGCTCCTGTTCGATCTGCTGTTCGAGGACATACAGCAGGt                                                                                                                                >  3:361669/1‑134 (MQ=255)                                           aTTTCCTTGCCGATAAACTCGATGTAGCGAGGTGCCAGGTATTCCTTCAGGTAGCGCAGATAGCGTTCGCGCACTTCAGCCGGGAATTGCTCCTGTTCGATCTGCTGTTCGAGGACATACAGCAGGTGCACCGGGTTggcg                                                                                                                  <  1:129903/141‑1 (MQ=255)                                           aTTTCCTTGCCGATAAACTCGATGTAGCGAGGTGCCAGGTATTCCTTCAGGTAGCGCAGATAGCGTTCGCGCACTTCAGCCGGGAATTGCTCCTGTTCGATCTGCTGTTCGAGGACATACAGCAGGTGCACCGGGTTggcg                                                                                                                  >  2:129903/1‑141 (MQ=255)                                             ttCCTTGCCGATAAACTCGATGTAGCGAGGTGCCAGGTATTCCTTCAGGTAGCGCAGATAGCGTTCGCGCACTTCAGCCGGGAATTGCTCCTGTTCGATCTGCTGTTCGAGGACATACAGCAGGTGCACCGGGTTGGCGGCAATTTCgt                                                                                                        >  3:9239/1‑149 (MQ=255)                                              tCCTTGCCGATAAACTCGATGTAGCGAGGTGCCAGGTATTCCTTCAGGTAGCGCAGATAGCGTTCGCGCACTTCAGCCGGGAATTGCTCCTGTTCGATCTGCTGTTCGAGGACATACAGCAGGTGCACCGGGTTGGCGGCAATTTCgt                                                                                                        <  5:154119/148‑1 (MQ=255)                                                    ccGATAAACTCGATGTAGCGAGGTGCCAGGTATTCCTTCAGGTAGCGCAGATAGCGTTCGCGCACTTCAGCCGGGAATTGCTCCTGTTCGATCTGCTGTTCGAGGACATACAGCAGGTGCACCGGGTTGGCGGCAATTTCGTGTGGGt                                                                                                  <  2:16210/148‑1 (MQ=255)                                                      gATAAACTCGATGTAGCGAGGTGCCAGGTATTCCTTCAGGTAGCGCAGATAGCGTTCGCGCACTTCAGCCGGGAATTGCTCCTGTTCGATCTGCTGTTCGAGGACATACAGCAGGTGCACCGGGTTGGCGGCAATTTCGTGTGGGTCg                                                                                                >  7:360384/1‑148 (MQ=255)                                                      gATAAACTCGATGTAGCGAGGTGCCAGGTATTCCTTCAGGTAGCGCAGATAGCGTTCGCGCACTTCAGCCGGGAATTGCTCCTGTTCGATCTGCTGTTCGAGGACATACAGCAGGTGCACCGGGTTGGCGGCAATTTCGTGTGGGTCGa                                                                                               >  7:97456/1‑149 (MQ=255)                                                      gATAAACTCGATGTAGCGAGGTGACAGGTATTCCTTCAGGCAGCGCAGATAGCGTTCGCGCACTTAAGCCGGGAATTGCTCCTGTTCGATCTGCTGTTCGAGGACATACAGCAGGTGCACCGGGTTGGCGGCAATTTCGTGTGGGTCg                                                                                                >  2:277861/1‑148 (MQ=255)                                                          aaCTCGATGTAGCGATGTGCCAGGTTTTCCTTCAGGTAGCTCAGATAGCGTTCGCGCACTTCAGCCGGGAATTGCTCCTGTTCTATATGCTGTTCGATGACATACAGCAGGTGCACCGGGTTGGCGGCAATTTCGTGTGGGTCGAAGt                                                                                            <  8:360384/148‑1 (MQ=255)                                                          aaCTCGATGTAGCGAGGTGCCAGGTATTCCTTCAGGTAGCGCAGATAGCGTTCGCGCACTTCAGCCGGGAATTGCTCCTGTTCGATCTGCTGTTCGAGGACATACAGCAGGTGCACCGGGTTGGCGGCAATTTCGTGTGGGTCGAAGt                                                                                            <  4:9239/148‑1 (MQ=255)                                                           aCTCGATGTAGCGAGGTGCCAGGTATTCCTTCAGGTAGCGCAGATAGCGTTCGCGCACTTCAGCCGGGAATTGCTCCTGTTCGATCTGCTGTTCGAGGACATACAGCAGGTGCACCGGGTTg                                                                                                                     >  4:163235/1‑122 (MQ=255)                                                           aCTCGATGTAGCGAGGTGCCAGGTATTCCTTCAGGTAGCGCAGATAGCGTTCGCGCACTTCAGCCGGGAATTGCTCCTGTTCGATCTGCTGTTCGAGGACATACAGCAGGTGCACCGGGTTg                                                                                                                     >  4:157267/1‑122 (MQ=255)                                                           aCTCGATGTAGCGAGGTGCCAGGTATTCCTTCAGGTAGCGCAGATAGCGTTCGCGCACTTCAGCCGGGAATTGCTCCTGTTCGATCTGCTGTTCGAGGACATACAGCAGGTGCACCGGGTTg                                                                                                                     <  3:157267/122‑1 (MQ=255)                                                           aCTCGATGTAGCGAGGTGCCAGGTATTCCTTCAGGTAGCGCAGATAGCGTTCGCGCACTTCAGCCGGGAATTGCTCCTGTTCGATCTGCTGTTCGAGGACATACAGCAGGTGCACCGGGTTg                                                                                                                     <  3:163235/122‑1 (MQ=255)                                                             tCGATGTAGCGAGGTGCCAGGTATTCCTTCAGGTAGCGCAGATAGCGTTCGCGCACTTCAGCCGGGAATTGCTCCTGTTCGATCTGCTGTTCGAGGACATACAGCAGGTGCACCGGGTTGGCGGCAATTTCGTGTGGGTCGAAGTTGaa                                                                                        >  3:123693/1‑149 (MQ=255)                                                              cGATGTAGCGAGGTGCCAGGTATTCCTTCAGGTAGCGCAGATAGCGTTCGCGCACTTCAGCCGGGAATTGCTCCTGTTCGATCTGCTGTTCGAGGACATACAGCAGGTGCACCGGGTTGGCGGCAATTTCGTGTGGGTCGAAGTTGaa                                                                                        >  8:147564/1‑148 (MQ=255)                                                               gATGTAGCGAGGTGCCAGGTATTCCTTCAGGTAGCGCAGATAGCGTTCGCGCACTTCAGCCGGGAATTGCTCCTGTTCGATCTGCTGTTCGAGGACATACAGCAGGTGCACCGGGTTGGCGGCAATTTCGTGTGGGTCGAAGTTGaa                                                                                        <  1:399640/147‑1 (MQ=255)                                                               gATGTAGCGAGGTGCCAGGTATTCCTTCAGGTAGCGCAGATAGCGTTCGCGCACTTCAGCCGGGAATTGCTCCTGTTCGATCTGCTGTTCGAGGACATACAGCAGGTGCACCGGGTTGGCGGCAATTTCGTGTGGGTCGAAGTTGAaca                                                                                      <  1:108250/149‑1 (MQ=255)                                                                     gCGAGGTGCCAGGTATTCCTTCAGGTAGCGCAGATAGCGTTCGCGCACTTCAGCCGGGAATTGCTCCTGTTCGATCTGCTGTTCGAGGACATACAGCAGGTGCACCGGGTTGGCGGCAATTTCGTGTGGGTCGAAGTTGAACACCTTgg                                                                                <  1:327236/149‑1 (MQ=255)                                                                        aGGTGCCAGGTATTCCTTCAGGTAGCGCAGATAGCGTTCGCGCACTTCAGCCGGGAATTGCTCCTGTTCGATCTGCTGTTCGAGGACATACAGCAGGTGCACCGGGTTGGCGGCAATTTCGTGTGGGTCGAAGTTGAACACCTTGGa                                                                               >  3:47414/1‑147 (MQ=255)                                                                         ggTGCCAGGTATTCCTTCAGGTAGCGCAGATAGCGTTCGCGCACTTCAGCCGGGAATTGCTCCTGTTCGATCTGCTGTTCGAGGACATACAGCAGGTGCACCGGGTTGGCGGCAATTTCGTGTGGGTCGAAGTTGAACACCTTGGACAg                                                                            >  1:160347/1‑149 (MQ=255)                                                                             ccAGGTATTCCTTCAGGTAGCGCAGATAGCGTTCGCGCACTTCAGCCGGGAATTGCTCCTGTTCGATCTGCTGTTCGAGGACATACAGCAGGTGCACCGGGTTGGCGGCAATTTCGTGTGGGTCGAAGTTGAACACCTTGGACAGGATc                                                                        >  4:300441/1‑149 (MQ=255)                                                                             ccAGGTATTCCTTCAGGCAGCGCAGATAGCGTTCGCGCACTTCAGCCGGTAATTGCTCCTGTTCGATCTGCTGTTCGATGACATACAGCAGGTTCACCGGGTTGGCGGCATTTTCGTGTGTGTCGAAGTTGAACACCTTGGACAGGATc                                                                        <  1:277861/149‑1 (MQ=255)                                                                              cAGGTATTCCTTCAGGTAGCGCAGATAGCGTTCGCGCACTTCAGCCGGGAATTGCTCCTGTTCGATCTGCTGTTCGAGGACATACAGCAGGTGCACCGGGTTGGAGGCAATTTCGTGTGGGTCGAAGTTGAACAACTTGGACAGGATCt                                                                       >  1:233147/1‑149 (MQ=255)                                                                               aGGTATTCCTTCAGGTAGCGCAGATAGCGTTCGCGCACTTCAGCCGGGAATTGCTCCTGTTCGATCTGCTGTTCGAGGACATACAGCAGGTGCACCGGGTTGGCGGCAATTTCGTGTGGGTCGAAGTTGAACACCTTGGACAGGATCtt                                                                      >  5:116948/1‑149 (MQ=255)                                                                                 gTATTCCTTCAGGTAGCGCAGATAGCGTTCGCGCACTTCAGCCGGGAATTGCTCCTGTTCGATCTGCTGTTCGAGGACATACAGCAGGTGCACCGGGTTGGCGGCAATTTCGTGTGGGTCGAAGTTGAACACCTTGGACAGGATCTTg                                                                     >  4:353078/1‑148 (MQ=255)                                                                                  tATTCCTTCAGGTAGCGCAGATAGCGTTCGGGCACTTCAGCCTGGAAATGCTCCTGTTCGATCTGCTGTTCGAGGACATACAGCAGGTGCACCGGGTTGGCGGCAATTTCGTGTTGGTCGAAGTTGAACACCTTTGACAGGATCTTGaa                                                                   <  2:10565/149‑1 (MQ=255)                                                                                  tATTCCTTCAGGTAGCGCAGATAGCGTTCGCTCACTTCAGCCGGTAATTGCTCCTGTTCGATCTGCTGTTCGAGGACATACAGCAGGTGCACCGGGTTGGCGGCAATTTCGTGTGGGTCGAAGTTGAACACCTTGGACAGGATCTTGaa                                                                   <  3:246462/149‑1 (MQ=255)                                                                                    ttCCTTCAGGTAGCGCAGATAGCGTTCGCGCACTTCAGCCGGGAATTGCTCCTGTTCGATCTGCTGTTCGAGGACATACAGCAGGTGCACCGGGTTGGCGGCAATTTCGTGTGGGTCGAAGTTGAACACCTTGGACAGGATCTTGAAgg                                                                 <  4:47414/149‑1 (MQ=255)                                                                                    ttCCTTCAGGTAGCGCAGATAGCGTTCGCGCACTTCAGCCGGGAATTGCTCCTGTTCGATCTGCTGTTCGAGGACATACAGCAGGTGCACCGGGTTGGCGGCAATTTCGTGTGGGTCGAAGTTGAACACCTTGGACAGGATCTTGAAgg                                                                 <  7:147564/149‑1 (MQ=255)                                                                                    ttCCTTCAGGTAGCGCAGATAGCGTTCGCGCACTTCAGCCGGGAATTGCTCCTGTTCGATCTGCTGTTCGAGGACATACAGCAGGTGCACCGGGTTGGCGGCAATTTCGTGTGGGTCGAAGTTGAACACCTTGGACAGGATCTTGAAg                                                                  <  5:252844/148‑1 (MQ=255)                                                                                        ttCAGGTAGCGCAGATAGCGTTCGCGCACTTCAGCCGGGAATTGCTCCTGTTCGATCTGCTGTTCGAGGACATACAGCAGGTGCACCGGGTTGGCGGCAATTTCGTGTGGGTCGAAGTTGAACACCTTGGACAGGATCTTGAAGGCg                                                               >  3:67039/1‑147 (MQ=255)                                                                                                gcgcAGATAGCGTTCGCGCACTTCAGCCGGGAATTGCTCCTGTTCGATCTGCTGTTCGAGGACATACAGCAGGTGCACCGGGTTGGCGGCAATTTCGTGTGGGTCGAAGtt                                                                                           >  7:126305/1‑111 (MQ=255)                                                                                                gcgcAGATAGCGTTCGCGCACTTCAGCCGGGAATTGCTCCTGTTCGATCTGCTGTTCGAGGACATACAGCAGGTGCACCGGGTTGGCGGCAATTTCGTGTGGGTCGAAGtt                                                                                           <  8:126305/111‑1 (MQ=255)                                                                                                gcgcAGATAGCGTTCGCGCACTTCAGCCGGGAATTGCTCCTGTTCGATCTGCTGTTCGAGGACATACAGCAGGTGCACCGGGTTGGCGGCAATTTCGTGTGGGTCGAAGTTGAACACCtt                                                                                  <  3:181478/120‑1 (MQ=255)                                                                                                gcgcAGATAGCGTTCGCGCACTTCAGCCGGGAATTGCTCCTGTTCGATCTGCTGTTCGAGGACATACAGCAGGTGCACCGGGTTGGCGGCAATTTCGTGTGGGTCGAAGTTGAACACCtt                                                                                  >  4:181478/1‑120 (MQ=255)                                                                                                 cgcAGATAGCGTTCGCGCACTTCAGCCGGGAATTGCTCCTGTTCGATCTGCTGTTCGAGGACATACAGCAGGTGCACCGGGTTGGCGGCAATTTCGTGTGGGTCGAAGTTGAACACCTTGGACAGGATCTTGAAGGCGAAGCGAGTCGa                                                    <  1:387298/149‑1 (MQ=255)                                                                                                 cgcAGATAGCGTTCGCGCACTTCAGCCGGGAATTGCTCCTGTTCGATCTGCTGTTCGAGGACATACAGCAGGTGCACCGGGTTGGCGGCAATTTCGTGTGGGTCGAAGTTGAACACCTTGGACAGGATCTTGAAGGCGAAGCGAGTCGa                                                    <  4:357158/149‑1 (MQ=255)                                                                                                 cgcAGATAGCGTTCGCGCACTTCAGCCGGGAATTGCTCCTGTTCGATCTGCTGTTCGAGGACATACAGCAGGTGCACCGGGTTGGCGGCAATTTCGTGTGGGTCGAAGTTGAACACCTTGGACAGGATCTTGAAGGCGAAGCGAGTCGa                                                    >  2:387298/1‑149 (MQ=255)                                                                                                   cAGATAGCGTTCGCGCACTTCAGCCGGGAATTGCTCCTGTTCGATCTGCTGTTCGAGGACATACAGCAGGTGCACCGGGTTGGCGGCAATTTCgtg                                                                                                       <  4:37279/96‑1 (MQ=255)                                                                                                   cAGATAGCGTTCGCGCACTTCAGCCGGGAATTGCTCCTGTTCGATCTGCTGTTCGAGGACATACAGCAGGTGCACCGGGTTGGCGGCAATTTCgtg                                                                                                       >  3:37279/1‑96 (MQ=255)                                                                                                   cAGATAGCGTTCGCGCACTTCAGCCGGGAATTGCTCCTGTTCGATCTGCTGTTCGAGGACATACAGCAGGTGCACCGGGTTGGCGGCAATTTCGTGTGGGTCGAAGTTGAACACCTTGGACAGGATCTTGAAGGCGAAGCGAGTCGACa                                                  <  3:353078/149‑1 (MQ=255)                                                                                                   cAGATAGCGTTCGCGCACTTCAGCCGGGAATTGCTCCTGTTCGATCTGCTGTTCGAGGACATACAGCAGGTGCACCGGGTTGGCGGCAATTTCGTGTGGGTCGAAGTTGAACACCTTGGACAGGATCTTGAAGGCGAAGCGAGTCGACa                                                  <  7:8705/149‑1 (MQ=255)                                                                                                           gTTCGCGCACTTCAGCCTGGAATTGCTCCTGTTCGATCTGTTGTTCGAGGACATACAGCAGGTGCACCGGGTTGGCGGCAATTTCGTGTGGGTCGAAGTTGAACACCTTGGACAGGATCTTGAAGGCGAAGCGAGTCGACAGGCCGTTc                                          <  3:115092/149‑1 (MQ=255)                                                                                                           gTTCGAGCAATTCAGCCGGGAATTGCTCCTGTTCGATCTGTTGTTCGAGGACATACATCAGGTGCACCGGGTTGGCGGCAATTTCGTGTTGGTCGAAGTTGAACACCTTGGACAGGATCTTGAAGGCGAAGCGAGTCGACAGGCCGTTc                                          <  3:109536/149‑1 (MQ=255)                                                                                                             tCGCGCACTTCAGCCGGTAATTGCTCCTGTTCGATCTGCTGTTCGAGGACATACAGCAGGTGCACCGGGTTGGCGGCAATTTCGTGTGGGTCGAATTTGAACACCTTGGACAGTATCTTGAAGGCGAAGCGAGTCGACAGGCCGTTCAt                                        <  7:257054/149‑1 (MQ=255)                                                                                                                cgcACTTCAGCCGGGAATTGCTCCTGTTCGATCTGCTGTTCGAGGACATACAGCAGGTGCACCGGGTTGGCGGCAATTTCGTGTGGGTCGAAGTTGAACACCTTGGACAGGATCTTGAAGGCGAAGCGAGTCGACAGGCCGTTCATGcc                                     >  2:4825/1‑149 (MQ=255)                                                                                                                     ttCAGCCGGGAATTGCTCCTGTTCGATCTGCTGTTCGAGGACATACAGCAGGTGCACCGGGTTGGCGGCAATTTCGTGTGGGTCGAAGTTGAACACCTTg                                                                                 >  8:71824/1‑100 (MQ=255)                                                                                                                     ttCAGCCGGGAATTGCTCCTGTTCGATCTGCTGTTCGAGGACATACAGCAGGTGCACCGGGTTGGCGGCAATTTCGTGTGGGTCGAAGTTGAACACCTTg                                                                                 <  7:71824/100‑1 (MQ=255)                                                                                                                          ccGGGAATTGCTCCTGTTCGATCTGCTGTTCGAGGACATACAGCAGGTGCACCGGGTTGGCGGCAATTTCGTGTGGGTCGAAGTTGAACACCTTGGACAGGATCTTGAAGGCGAAGCGAGTCGACAGGCCGTTCAt                                        <  7:354300/136‑1 (MQ=255)                                                                                                                          ccGGGAATTGCTCCTGTTCGATCTGCTGTTCGAGGACATACAGCAGGTGCACCGGGTTGGCGGCAATTTCGTGTGGGTCGAAGTTGAACACCTTGGACAGGATCTTGAAGGCGAAGCGAGTCGACAGGCCGTTCAt                                        >  8:354300/1‑136 (MQ=255)                                                                                                                                   gCTCCTGTTCGATCTGCTGTTCGAGGACATACAGCAGGTGCACCGGGTTGGCGGCAATTTCGTGTGGGTCGAAGTTGAACACCTTGGACAGGATCTTGAAGGCGAAGCGAGTCGACAGGCCGTTCATGCCTTCGTCGACACCCGCaga                   <  3:353266/148‑1 (MQ=255)                                                                                                                                    cTCCTGTTCGATCTGCTGTTCGAGGACATAAAGCAGGTGCACCGGGTTGGCGGCAATTTCGTGTGGGTCGAAGTTGAACACCTTGGACAGGATCTTGAAGGCGAAGCGAGTCGACAGGCCGTTCATGCCTTCGTCGACACCCGCAGAGt                 >  6:239805/1‑149 (MQ=255)                                                                                                                                      ccTGTTCGATCTGCTGTTCGAGGACATACAGCAGGTGCACCGGGTTGGCGGCAATTTCGTGTGGGTCGAAGTTGAACACCTTGGACAGGATCTTGAAGGCGAAGCGAGTCGACAg                                                 >  3:83389/1‑115 (MQ=255)                                                                                                                                      ccTGTTCGATCTGCTGTTCGAGGACATACAGCAGGTGCACCGGGTTGGCGGCAATTTCGTGTGGGTCGAAGTTGAACACCTTGGACAGGATCTTGAAGGCGAAGCGAGTCGACAg                                                 <  4:83389/115‑1 (MQ=255)                                                                                                                                      ccTGTTCGATCTGCTGTTCGAGGACATACAGCAGGTGCACCGGGTTGGCGGCAATTTCGTGTGGGTCGAAGTTGAACACCTTGGACAGGATCTTGAAGGCGAAGCGAGTCGACAGGCCGTTCATGCCTTCGTCGACACCCGCAGAGTcc               >  4:381342/1‑149 (MQ=255)                                                                                                                                                 tgctgTTCGAGGACATACAGCAGGTGCACCGGGTTGGCGGCAATTTCGTGTGGGTCGAAGTTGAACACCTTGGACAGGATCTTGAAGGCGAAGCGAGTCGACAGGCCGTTCATGCCTTCGTCGACACCCGCAGAGTCCCGGTATTCCTg    >  6:85430/1‑149 (MQ=255)                                                                                                                                                   ctgTTCGAGGACATACAGCAGTTGAACCGGGTTTGCGGCAATTTCGTGTGGGTCGATGTAAAACACCATGGACAGGATCTAGAAGGCGAAGCGAGTCGACAGGCAAATCATGCCTTCGTCTACACCCGCACAGTCCCGGTATTCCTGGa  >  6:88793/1‑149 (MQ=255)                                                                                                                                                   ctgTTCGAGGACATACAGCAGGTGCACCGGGTTGGCGGCAATTTCGTGTGGGTCGAAGTTGAACACCTTGGACAGGATCTTGAAGGCGAAGCGAGTCGACAGGCCGTTCATGCCTTCGTCGACACCCGCAGAGTCCCGGTATTCCt     <  1:4825/146‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                     TTCTGGCCGTATTCGCTGTAGGACTCCAGGTAGGCAGTCTGGATTTCCTTGCCGATAAACTCGATGTAGCGAGGTGCCAGGTATTCCTTCAGGTAGCGCAGATAGCGTTCGCGCACTTCAGCCGGGAATTGCTCCTGTTCGATCTGCTGTTCGAGGACATACAGCAGGTGCACCGGGTTGGCGGCAATTTCGTGTGGGTCGAAGTTGAACACCTTGGACAGGATCTTGAAGGCGAAGCGAGTCGACAGGCCGTTCATGCCTTCGTCGACACCCGCAGAGTCCCGGTATTCCTGGA  >  NC_002947/482949‑483243

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑