Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,776,248	A→T	8.1%	M189L (ATG→TTG) ‡	betA‑II →	choline dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,776,248	0	A	T	8.1%	237.6 / 7.7	83	M189L (ATG→TTG) ‡	betA‑II	choline dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (40/36);  new base T (2/5);  total (42/41)
Fisher's exact test for biased strand distribution p-value = 2.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.43e-01

CCATGGCGGCGAAGGCCCGGTCAGCGTGACCACGCCGAAAGCCGGCAACAACCCGCTGTTCCATGCCATGGTCGAAGCTGGCGTACAGGCCGGTTACCCACGCACAG‑AAGACCTCAACGGTTACCAGCAGGAAGGCTTCGGTCCGATGGACCGCACCGTGACCAAGAACGGCCGCCGCTCCAGCACTGCCCGTGGTTACCTGGACCAGGCCAAGAAGCGCCCGAACCTGACCATCGTCACCCACGCCCTCACGGATCGCGTTCTGTTCGACGGCAAGCGCGCCATCGGCG  >  NC_002947/5776103‑5776392                                                                                                                                                   |                                                                                                                                                 ccATGGCGGCGAAGGCCCGGTCAGCGTGACCACGCCGAAAGCCGGCAACAACCCGCTGTTCCATGCCATGGTCGAAGCTGGCGTACAGGCCGGTTACCCACGCACAG‑AAGACCTCAACGGTTACCAGCAGGAAGGCTTCGGTCCGATg                                                                                                                                                >  1:236472/1‑148 (MQ=255) ccATGGCGGCGAAGGCCCGGTCAGCGTGACCACGCCGAAAGCCGGCAACAACCCGCTGTTCCATGCCATGGTCGAAGCTGGCGTACAGGCCGGTTACCCACGCACAG‑AAGACCTCAACGGTTACCAGCAGGAAGGCTTCGGTCCGATg                                                                                                                                                <  1:27768/148‑1 (MQ=255) ccATGGCGGCGAAGGCCCGGTCAGCGTGACCACGCCGAAAGCCGGCAACAACCCGCTGTTCCATGCCATGGTCGAAGCTGGCGTACAGGCAGGTTACCCACGCACAG‑AAGACCTCAACGGTTACCAGCAGGAAGGCTTCGGTCCGATg                                                                                                                                                >  5:60394/1‑148 (MQ=255)  cATGGCGGCGAAGGCCCGGTCAGCGTGACCACGCCGAAAGCCGGCAACAACCCGCTGTTCCATGCCATGGTCGAAGCTGGCGTACAGGCCGGTTACCCACGCACAG‑AAGACCTCAACGGTTACCAGCAGGAAGGCTTCGGTCCGATGGa                                                                                                                                              >  1:375858/1‑149 (MQ=255)   aTGGCGGCGAAGGCCCGGTCAGCGTGACCACGCCGAAAGCCGGCAACAACCCGCTGTTCCATGCCATGGTCGAAGCTGGCGTACAGGCCGGTTACCCACGCACAG‑AAGACCTCAACGGTTACCAGCAGGAAGGCTTCGGTCCGATGGAc                                                                                                                                             <  5:278624/149‑1 (MQ=255)   aTGGCGGCGAAGGCCCGGTCAGCGTGACCACGCCGAAAGCCGGCAACAACCCGCTGTTCCATGCCATGGTCGAAGCTGGCGTACAGGCCGGTTACCCACGCACAG‑AAGACCTCAACGGTTACCAGCAGGAAGGCTTCGGTCCGATGGAc                                                                                                                                             <  7:29497/149‑1 (MQ=255)    tGGCGGCGAAGGCCCGGTCAGCGTGACCACGCCGAAAGCCGGCAACAACCCGCTGTTCCATGCCATGGTCGAAGCTGGCGTACAGGCCGGTTACCCACGCACAG‑AAGACCTCAACGGTTACCAGCAGGATGGCTTCGGTCCGATGGAcc                                                                                                                                            >  6:336907/1‑149 (MQ=255)       cggcgAAGGCCCGGTCAGCGTGACCACGCCGAAAGCCGGCAACAACCCGCTGTTCCATGCCATGGTCGAAGCTGGCGTACAGGCCGGTTACCCACGCACAGCAAGACCTCAACGGTTACCAGCAGGAAGGCTTCGGTCCGATGGACCGc                                                                                                                                          <  1:294175/149‑1 (MQ=255)       cggcgAAGGCCCGGTCAGCGTGACCACGCCGAAAGCCGGCAACAACCCGCTGTTCCATGCCATGGTCGAAGCTGGCGTACAGGCCGGTTACCCACGCACAG‑AAGACCTCAACGGTTACCAGCAGGAAGGCTTCGGTCCATCGGACCgaa                                                                                                                                         <  2:131657/149‑3 (MQ=255)        ggcgAAGGCCCGGTCAGCGTGACCACGCCGAAAGCCGGCAACAACCCGCTGTTCCATGCCATGGTCGAAGCTGGCGTACAGGCCGGTTACCCACGCACAG‑AAGACCTCAACGGTTACCAGCAGGAAGGCTTCGGTCCATCGGACCgaag                                                                                                                                        <  8:319815/149‑4 (MQ=255)          cgAAGGCCCGGTCAGCGTGACCACGCCGAAAGCCGGCAACAACCCGCTGTTCCATGCCATGGTCGAAGCTGGCGTACAGGCCGGTTACCCACGCACAG‑AAGACCTCAACGGTTACCAGCAGGAAGGCTTCGGTCCGATGGACCGCACCg                                                                                                                                      >  1:300342/1‑149 (MQ=255)           gAAGGCCCGGTCAGCGTGACCACGCCGAAAGCCGGCAACAACCCGCTGTTCCATGCCATGGTCGAAGCTGGCGTACAGGCCGGTTACCCACGCACAG‑AAGACCTCAACGGTTACCAGCAGGAAGGCTTCGGTCCGATGGACCGCACCGt                                                                                                                                     >  1:183650/1‑149 (MQ=255)            aaGGCCCGGTCAGCGTGACCACGCCGAAAGCCGGCAACAACCCGCTGTTCCATGCCATGGTCGAAGCTGGCGTACAGGCCGGTTACCCACGCACAG‑AAGACCTCAACGGTTACCAGCAGGAAGGCTTCGGTCCGATGGACCGCACCGTg                                                                                                                                    <  2:183650/149‑1 (MQ=255)              ggCCCGGTCAGCGTGACCACGCCGAAAGCCGGCAACAACCCGCTGTTCCATGCCATGGTCGAAGCTGGCGTACAGGCCGGTTACCCACGCACAG‑AAGACCTCAACGGTTACCAGCAGGAAGGCTTCGGTCCGATGGACCGCACCGTGAc                                                                                                                                  >  6:361636/1‑149 (MQ=255)                   ggTCAGCGTGACCACGCCGAAAGCCGGCAACAACCCGCTGTTCCATGCCATGGTCGAAGCTGGCGTACAGGCCGGTTACCCACGCACAG‑AAGACCTCAACGGTTACCAGCAGGAAGGCTTCGGTCCGATGGACCGCACCGTGACCAAg                                                                                                                              >  2:298975/1‑148 (MQ=255)                       aGCGTGACCACGCCGAAAGCCGGCAACAACCCGCTGTTCCATGCCATGGTCGAAGCTGGCGTACAGGCCGGTTACCCACGCACAG‑AAGACCTCAACGGTTACCAGCAGGAAGGCTTCGGTCCGATGGACCGCACCGTGACCAAGAACg                                                                                                                          <  5:361636/148‑1 (MQ=255)                         cGTGACCACGCCGAAAGCCGGCAACAACCCGCTGTTCCATGCCATGGTCGAAGCTGGCGTACAGGCCGGTTACCCACGCACAG‑AAGACCTCAACGGTTACCAGCAGGAAGTCTTCGTTCCGATGGACCGCACCGTGACCAAGAACGgcc                                                                                                                       <  3:342914/149‑1 (MQ=255)                              ccACGCCGAAAGCCGGCAACAACCCGCTGTTCCATGCCATGGTCGAAGCTGGCGTACAGGCCGGTTACCCACGCACAG‑AAGACCTCAACGGTTACCAGCAGGAAGGCTTCGGTCCGATGGACCGCACCGTGACCAAGAACGGCcgccgc                                                                                                                  >  3:101871/1‑149 (MQ=255)                               cACGCCGAAAGCCGGCAACAACCCGCTGTTCCATGCCATGGTCGAAGCTGGCGTACAGGCCGGTTACCCACGCACAG‑AAGACCTCAACGGTTACCAGCAGGAAGGCTTCGGTCCGATGGACCGCACCGTGACCAAGAACGGCCGCCGCt                                                                                                                 >  1:242880/1‑149 (MQ=255)                               cACGCCGAAAGCCGGCAACAACCCGCTGTTCCATGCCATGGTCGAAGCTGGCGTACAGGCCGGTTACCCACGCACAG‑AAGACCTCAACGGTTACCAGCAGGAAGGCTTCGGTCCATCGGACCgaa                                                                                                                                         <  1:397236/125‑3 (MQ=255)                               cACGCCGAAAGCCGGCAACAACCCGCTGTTCCATGCCATGGTCGAAGCTGGCGTACAGGCCGGTTACCCACGCACAG‑AAGACCTCAACGGTTACCAGCAGGAAGGCTTCGGTCCATCGGACCgaa                                                                                                                                         >  2:397236/1‑123 (MQ=255)                                aCGCCGAAAGCCGGCAACAACCCGCTGTTCCATGCCATGGTCGAAGCTGGCGTACAGGCCGGTTACCCACGCACAG‑AAGACCTCAACGGTTACCAGCAGGAAGGCTTCGGTCCGATGGACCGCACCGTGACCAAGAACGGCCGCCGCTc                                                                                                                <  4:185254/149‑1 (MQ=255)                                  gCCGAAAGCCGGCAACAACCCGCTGTTCCATGCCATGGTCGAAGCTGGCGTACAGGCCGGTTACCCACGCACAG‑AAGACCTCAACGGTTACCAGCAGGAAGGCTTCGGTCCGATGGACCGCACCGTGACCAAGAACGGCCGCCGCTCCa                                                                                                              <  6:40465/149‑1 (MQ=255)                                  gCCGAAAGCCGGCAACAACCCGCTGTTCCATGCCATGGTCGAAGCTGGCGTACAGGCCGGTTACCCACGCACAG‑AAGACCTCAACGGTTACCAGCAGGAAGGCTTCGGTCCGATGGACCGCACCGTGACCAAGAACGGCCGCCGCTCCa                                                                                                              <  2:300342/149‑1 (MQ=255)                                  gCCGAAAGCCGGCAACAACCCGCTGTTCCATGCCATGGTCGAAGCTGGCGTACAGGCCGGTTACCCACGCACAG‑AAGACCTCAACGGTTACCAGCAGGAAGGCTTCGGTCCGATGGACCGCACCGTGACCAAGAACGGCCGCCGCTCCa                                                                                                              <  2:242880/149‑1 (MQ=255)                                    cGAAAGCCGGCAACAACCCGCTGTTCCATGCCATGGTCGAAGCTGGCGTACAGGCCGGTTACCCACGCACAG‑AAGACCTCAACGGTTACCAGCAGGAAGGCTTCGGTCCGATGGACCGCACCGTGACCAAGAACGGCCGCCGCTCCAg                                                                                                             >  2:63913/1‑148 (MQ=255)                                     gAAAGCCGGCAACAACCCGCTGTTCCATGCCATGGTCGAAGCTGGCGTACAGGCCGGTTACCCACGCACAG‑AAGACCTCAACGGTTACCAGCAGGAAGGCTTCGGTCCGATGGACCGCACCGTGACCAAGAACGGCCGCCGCTCCAGCa                                                                                                           <  2:375858/149‑1 (MQ=255)                                       aaGCCGGCAACAACCCGCTGGTCCATGCCATGGTCGAAGCTGGCGTACAGGCCGGTTACCCACGCACAG‑AAGACCTCAACGGTTACCAGCAGGAAGGCTTCGGTCCGATGGACCGCACCGTGACCAAGAACGGCCGCCGCTCCAGCACt                                                                                                         >  1:269300/1‑149 (MQ=255)                                           cGGCAACAACCCGCTGTTCCATGCCATGGTCGAAGCTGGCGTACAGGCCGGTTACCCACGCACAG‑AAGACCTCAACGGTTACCAGCAGGAAGGCTTCGGTCCGATGGACCGCACCGTGACCAAGAACGGCCGCCGCTCCAGCACTGccc                                                                                                     >  2:91833/1‑149 (MQ=255)                                              caacaaCCCGCTGTTCCATGCCATGGTCGAAGCTGGCGTACAGGCCGGTTACCCACGCACAG‑AAGACCTCAACGGTTACCAGCAGGAAGGCTTCGGTCCGATGGACCGCACCGTGACCAAGAACGGCCGCCGCTCCAGCACTGCCCGTg                                                                                                  <  2:116491/149‑1 (MQ=255)                                                   acCCGCTGTTCCATGCCATGGTCGAAGCTGGCGTACAGGCCGGTTACCCACGCACAG‑AAGACCTCAACGGTTACCAGCAGGAAGGCTTCGGTCCATCGGACCgaa                                                                                                                                         <  2:317072/105‑3 (MQ=255)                                                   acCCGCTGTTCCATGCCATGGTCGAAGCTGGCGTACAGGCCGGTTACCCACGCACAG‑AAGACCTCAACGGTTACCAGCAGGAAGGCTTCGGTCCATCGGACCgaa                                                                                                                                         >  1:317072/1‑103 (MQ=255)                                                        cTGTTCCATGCCATGGTCGAAGCTGGCGTACAGGCCGGTTACCCACGCACAG‑AAGACCTCAACGGTTACCAGCAGGAAGGCTTCGGTCCGATGGACCGCACCGTGACCAAGAACGGCCGCCGCTCCAGCACTGCCCGTGGTTACCTGGa                                                                                        <  2:269300/149‑1 (MQ=255)                                                           ttCCATGCCATGGTCGAAGCTGGCGTACAGGCCGGTTACCCACGCACAG‑AAGACCTCAACGGTTACCAGCAGGAAGGCTTCGGTCCGATGGACCGCACCGTGACCAAGAACGGCCGCCGCTCCAGCACTGCCCGTGGTTACCTGGAc                                                                                       <  2:236472/147‑1 (MQ=255)                                                              catgccatgGTCGAAGCTGGCGTACCGGCCGGTTACCCACGCACAG‑AAGACCTCAACGGTTACCAGCAGGAAGGCTTCGGTCCGATGGACCGCACCGTGACCAAGAACGGCCGCCGCTCCAGCACTGCCCGTGGTTACCTGGACCAgg                                                                                   <  4:306296/148‑1 (MQ=255)                                                              catgccatgGTCGAAGCTGGCGTACAGGCCGGTTACCCACGCACAG‑AAGACCTCAACGGTTACCAGCAGGAAGGCTTCGGTCCGATGGACCGCACCGTGACCAAGAACGGCCGCCGCTCCAGCACTGCCCGTGGTTACCTGGACCAGGc                                                                                  >  4:38213/1‑149 (MQ=255)                                                                    atgGTCGAAGCTGGCGTACAGGCCGGTTACCCACGCACAG‑AAGACCTCAACGGTTACCAGCAGGAAGGCTTCGGTCCGATGGACCGCACCGTGACCAAGAACGGCCGCCGCTCCAGCACTGCCCGTGGTTACCTGGACCAGGCCaag                                                                              >  6:85612/1‑147 (MQ=255)                                                                     tgGTCGAAGCTGGCGTACAGGCCGGTTACCCACGCACAG‑AAGACCTCAACGGTTACCAGCAGGAAGGCTTCGGTCCGATGGACCGCACCGTGACCAAGAACGGCCGCCGCTCCAGCACTGCCCGTGGTTACCTGGACCAGGCCaagaag                                                                           >  6:144000/1‑149 (MQ=255)                                                                       gTCGAAGCTGGCGTACAGGCCGGTTACCCACGCACAG‑AAGACCTCAACGGTTACCAGCAGGAAGGCTTCGGTCCGATGGACCGCACCGTGACCAAGAACGGCCGCCGCTCCAGCACTGCCCGTGGTTACCTGGACCAGGCCAAGAAgcg                                                                         >  3:317540/1‑149 (MQ=255)                                                                       gTCGAAGCTGGCGTACAGGCCGGTTACCCACGCACAG‑AAGACCTCAACGGTTACCAGCAGGAAGGCTTCGGTCCGATGGACCGCACCGTGACCAAGAACGGCCGCCGCTCCAGCACTGCCCGTGGTTACCTGGACCAGGCCAAGAAgc                                                                          <  7:303827/148‑1 (MQ=255)                                                                                  cGTACAGGCCGGTTACCCACGCACAG‑AAGACCTCAACGGTTACCAGCAGGAAGGCTTCGGTCCGATGGACCGCACCGTGACCAAGAACGGCCGCCGCTCCAGCACTGCCCGTGGTTACCTGGACCAGGCCAAGAAGCGCCCGAACCTGa                                                              <  1:298975/149‑1 (MQ=255)                                                                                         gaacattACCCACGCACAC‑AAGACCTCAACGTATACCAGCAGAAAGCATACCATCAGATTGACATCACATATACCAACAACCTCCGACGCTCCATCACAGAACGTCATTACCTCTAACAGAACAATAAGCGCCCGAACCTGACCATCg                                                        >  6:302447/6‑148 (MQ=255)                                                                                         accGGTTACACACGCACAG‑AAGACCACAACCGTTACCAGCAGGAAGGCTTCGGTCCGATCGACCGCACCGTGACCAAGAACTGCCGCCGCTCCAGCACTGCCCATGGTTACCTGGACCAGGCGAAGAAGAGCCCGAAACAGACCATCga                                                       >  2:316914/2‑148 (MQ=255)                                                                                            ggTTACCCACGCACAG‑AAGACCTCAACGGTTACCAGCAGGAAGGCTTCGGTCCGATGGACCGCACCGTGACCAAGAACGGCCGCCGCTCCAGCACTGCCCGTGGTTACCTGGACCAGGCCAAGAAGCGCCCGAACCTGACCATCGTCAc                                                    >  7:153031/1‑149 (MQ=255)                                                                                                  ccACGCACAG‑AAGACCTCAACGGTTACCAGCAGGAAGGCTTCGGTCCGATGGACCGCACCGTGACCAAGAACGGCCGCCGCTCCAGCACTGCCCGTGGTTACCTGGACCAGGCCAAGAAGCGCCCGAACCTGACCa                                                           >  5:203220/1‑136 (MQ=255)                                                                                                  ccACGCACAG‑AAGACCTCAACGGTTACCAGCAGGAAGGCTTCGGTCCGATGGACCGCACCGTGACCAAGAACGGCCGCCGCTCCAGCACTGCCCGTGGTTACCTGGACCAGGCCAAGAAGCGCCCGAACCTGACCa                                                           <  6:203220/136‑1 (MQ=255)                                                                                                  ccACGCACAG‑AAGACCTCAACGGTTACCAGCAGGAAGGCTTCGGTCCGATGGACCGCACCGTGACCAAGAACGGCCGCCGCTCCAGCACTGCCCGTGGTTACCTGGACCAGGCCAAGAAGCGCCCGAACCTGACCATCGTCACCCACGc                                              >  5:27426/1‑149 (MQ=255)                                                                                                   cACGCACAG‑AAGACCTCAACGGTTACCAGCAGGAAGGCTTCGGTCCGATGGACCGCACCGTGACCAAGAACGGCCGCCGCTCCAGCACTGCCCGTGGTTACCTGGACCAGGCCAAGAAGCGCCCGAACCTGACCATCGTCACCCACGcc                                             <  3:38213/149‑1 (MQ=255)                                                                                                        acaG‑AAGACCTCAACGGTTACCAGCAGGAAGGCTTCGGTCCGATGGACCGCACCGTGACCAAGAACGGCCGCCGCTCCAGCACTGCCCGTGGTTACCTGGACCAGGCCAAGAAGCGCCCGAACCTGACCATCGTCACCCACGCCCTCAc                                        >  1:10997/1‑149 (MQ=255)                                                                                                           g‑aagaCCTCAACGGTTACCAGCAGGAAGGCTTCGGTCCGATGGACCGCACCGTGACCAAGAACGGCCGCCGCTCCAGCACTGCCCGTGGTTACCTGGACCAGGCCAAGAAgc                                                                          <  7:172278/112‑1 (MQ=255)                                                                                                           g‑aagaCCTCAACGGTTACCAGCAGGAAGGCTTCGGTCCGATGGACCGCACCGTGACCAAGAACGGCCGCCGCTCCAGCACTGCCCGTGGTTACCTGGACCAGGCCAAGAAgc                                                                          >  8:172278/1‑112 (MQ=255)                                                                                                                 ccTCAACGGTTCCCAGCAGGAAGGCTTCGGTCCGATGGACCGCACCGTGACCAAGAACGGCCGCCGCTCCAGCACTGCCCGTGGTTACCTGGACCATGCCAAGAATCGCCCGAACCTGACCATCGTCACCCACGCCCTCACGGATcgcg                                <  4:110201/149‑1 (MQ=255)                                                                                                                 ccTCAACGGTTACCAGCAGGAAGGCTTCGGTCCGATGGACCGCACCGTGACCAAGAACGGCCGCCGCTCCAGCACTGCCCGTGGTTACCTGGACCAGGCCAAGAAGCGCCCGAACCTGACCATCGTCACCCACGCCCTCACGGATcgc                                 <  8:153031/148‑1 (MQ=255)                                                                                                                 ccTCAACGGTTACCAGCAGGAAGGCTTCGGTCCGATGGACCGCACCGTGACCAAGAACGGCCGCCGCTCCAGCACTGCCCGTGGTTACCTGGACCAGGCCAAGAAGCGCCCGAACCTGACCATCGTCACCCACGCCCTCACGGATc                                   <  1:273587/146‑1 (MQ=255)                                                                                                                 ccTCAACGGTTACCAGCAGGAAGGCTTCGGTCCGATGGACCGCACCGTGACCAAGAACGGCCGCCGCTCCAGCACTGCCCGTGGTTACCTGGACCAGGCCAAGAAGCGCCCGAACCTGACCATCGTCACCCACGCCCTCACGGATc                                   >  2:273587/1‑146 (MQ=255)                                                                                                                  cTCAACGGTTACCAGCAGGAAGGCTTCGGTCCGATGGACCGCACCGTGACCCCGAACGGCCGCCGCTCCAGCACTGCCCGTGGTTACCTGGACCAGGCCAAGAAGCGCCCGAACCTGACCATCGTCACCCACGCCCTCACGGATCGCGt                               <  1:335676/149‑1 (MQ=255)                                                                                                                  cTCAACGGTTACCAGCAGGAAGGCTTCGGTCCGATGGACCGCACCGTGACCAAGAACGGCCGCCGCTCCAGCACTGCCCGTGGTTACCTGGACCAGGCCAAGAAGCGCCCGAACCTGACCATCGTCACCCACGCCCTCACGGATCGCGt                               <  3:105742/149‑1 (MQ=255)                                                                                                                  cTCAACGGTTACCAGCAGGAAGGCTTCGGTCCGATGGACCGCACCGTGACCAAGAACGGCCGCCGCTCCAGCACTGCCCGTGGTTACCTGGACCAGGCCAAGAAGCGCCCGAACCTGACCATCGTCACCCACGCCCTCACGGATCGCGt                               <  1:63913/149‑1 (MQ=255)                                                                                                                   tCAACGGTTACCAGCAGGAAGGCTTCGGTCCGATGGACCGCACCGTGACCAAGAACGGCCGCCGCTCCAGCACTGCCCGTGGTTACCTGGACCAGGCCAAGAAGCGcc                                                                       <  5:332051/108‑1 (MQ=255)                                                                                                                   tCAACGGTTACCAGCAGGAAGGCTTCGGTCCGATGGACCGCACCGTGACCAAGAACGGCCGCCGCTCCAGCACTGCCCGTGGTTACCTGGACCAGGCCAAGAAGCGcc                                                                       >  6:332051/1‑108 (MQ=255)                                                                                                                   tCAACGGTTACCAGCAGGAAGGCTTCGGTCCGATGGACCGCACCGTGACCAAGAACGGCCGCCGCTCCAGCACTGCCCGTGGTTACCTGGACCAGGCCAAGAAGCGCCCGAACCTGACCATCGTCACCCACGCCCTCACGGATCGCGtt                              >  2:331723/1‑149 (MQ=255)                                                                                                                   tCAACGGTTACCAGCAGGAAGGCTTCGGTCCGATGGACCGCACCGTGACCAAGAACGGCCGCCGCTCCAGCACTGCCCGTGGTTACCTGGACCAGGCCAAGAAGCGCCCGAACCTGACCATCGTCACCCACGCCCTCACGGATCGCGtt                              >  4:34359/1‑149 (MQ=255)                                                                                                                     aaCGGTTACCAGCAGGAAGGCTTCGGTCCGATGGACCGCACCGTGACCAAGAACGGCCGCCGCTCCAGCACTGCCCGTGGTTACCTGGACCAGGCCAAGAAGCGCCCGAACCTGACCATCGTCACCCACGCCCTCACGGATCGCGtt                              <  4:101871/147‑1 (MQ=255)                                                                                                                      aCGGTTACCAGCTGGAAGGCTTCGGTCCGTTTGACCGCACCGTGACCAAGAACGGCGGCCGCTCCAGCACTGCCCGTGGTTACCTGGACCAGGCCAAGAAGCGCCCGAACCTGACCATCGTCACCCACGCCCTCACGGATCGCGTTCTg                           <  5:182313/149‑1 (MQ=255)                                                                                                                       cGGTTACCAGCAGGAAGGCTTCGGTCCGATGGACCGCACCGTGACCAAGAACGGCCGCCGCTCCAGCACTGCCCGGGGTTACGTGGACCAGGGCAAGAAGCGCCCGCACCTGTCCCTAGTCACCCCCGCCCTCACGGATCGCGTTCTgg                          >  4:368123/1‑148 (MQ=255)                                                                                                                        ggTTACCAGCAGGAAGGCTTCGGTCCGATGGACCGCACCGTGACCAAGAACGGCCGCCGCTCCAGCACTGCCCGTGGTTACCTGGAc                                                                                       >  6:410200/1‑87 (MQ=255)                                                                                                                        ggTTACCAGCAGGAAGGCTTCGGTCCGATGGACCGCACCGTGACCAAGAACGGCCGCCGCTCCAGCACTGCCCGTGGTTACCTGGAc                                                                                       <  5:410200/87‑1 (MQ=255)                                                                                                                            aCCAGCAGGAAGGCTTCGGTCCGATGGACCGCACCGTGACCAAGAACGGCCGCCGCTCCAGCACTGCCCGTGGTTACCTGGACCAGGCCAAGAAGCGCCCGAACCTGACCATCGTCACCCACGCCCTCACGGATCGCGTTCTGTTCGAc                     >  3:347607/1‑149 (MQ=255)                                                                                                                             ccAGCAGGAAGGCTTCGGTCCGATGGACCGCACCGTGACCAAGAACGGCCGCCGCTCCAGCACTGCCCGTGGTTACCTGGACCAGGCCAAGAAGCGCCCGAACCTGACCATCGTCACCCACGCCCTCACGGATCGCGTTCTGTTCGAc                     <  2:10997/148‑1 (MQ=255)                                                                                                                               agcagGAAGGCTTCGGTCCGATGGACCGCACCGTGACCAAGAACGGCCGCCGCTCCAGCACTGCCCGTGGTTACCTGGACCAGGCCAAGAAGCGCCCGAACCTGACCATCGTCACCCACGCCCTCACGGATCGCGTTCTGTTCGACgg                   >  7:105214/1‑148 (MQ=255)                                                                                                                                gcagGAAGGCTTCGGTCCGATGGACCGCACCGTGACCAAGAACGGCCGCCGCTCCAGCACTGCCCGTGGTTACCTGGACCAGGCCAAGAAGCGCCCGAACCTGACCATCGTCCCCCACGCCCTCACGGATCGCGTTCTGTTCGACGGc                  >  2:4736/1‑148 (MQ=255)                                                                                                                                 catgAAGCCTTGGGTCCGATGGACCGCACCGTGACCAAGAACGGCAGCCGCTCCAGCACTGCCCGTGGTTACGTGGACCAGGCCTAGAAGCGCCCGAACCTGACCATCTTCACCCACGCCCTCACGGATCGCGTTCTGTTCGACGGCaa                <  1:316914/146‑1 (MQ=255)                                                                                                                                 cagGAATGCTTCGGTCCGATGGACCGCACCGTGACCAAGAACGGCCGCCGCTCCAGCACTTCCCGTGGTTACCTGGACCAGGCCAAGAAGCGCCCGAACCTGACCATCGTCACCCACGCCCTCACGGATCGCGTTCTGTTCGACGGCaa                <  5:302447/149‑1 (MQ=255)                                                                                                                                    gAAGGCTTCGGTCCGATGGACCGCACCGTGACCAAGAACGGCCGCCGCTCCAGCACTGCCCGTGGTTACCTGGACCAGGCCAAGAAGCGCCCGAACCTGACCATCGTCACCCACGCCCTCACGGATCGCGTTCTGTTCGACGGCAAgcg             >  4:10620/1‑149 (MQ=255)                                                                                                                                       ggCTTCGGTCCGATGGACCGCACCGTGACCAAGAACGGCCGCCGCTCCAGCACTGCCCGTGGTTACCTGGACCAGGCCAAGAAGCGCCCGAACCTGACCATCGTCACCCACGCCCTCACGGATCGCGTTCTGTTCGACGGCAAgcgcgc          <  1:91833/149‑1 (MQ=255)                                                                                                                                          ttCGGTCCGATGGACCGCACCGTGACCAAGAACGGCCGCCGCTCCAGCACTGCCCGTGGTTACCTGGACCAGGCCAAGAAGCGCCCGAACCTGACCATCGTCACCCACGCCCTCCCGGATCGCGTTCTGTTCGACGGCAAGCGCGCCAt       >  7:384726/1‑149 (MQ=255)                                                                                                                                          ttCGGTCCGATGGACCGCACCGTGACCAAGAACGGCCGCCGCTCCAGCACTGCCCGTGGTTACCTGGACCAGGCCAAGAAGCGCCCGAACCTGACCATCGTCACCCACGCCCTCACGGATCGCGTTCTGTTCGACGGCAAGCGCGCCAt       >  5:109399/1‑149 (MQ=255)                                                                                                                                          ttCGGTCCGATGGACCGCACCGTGACCAAGAACGGCCGCCGCTCCAGCACTGCCCGTGGTTACCTGGACCAGGCCAAGAAGCGCCCGAACCTGACCATCGTCACCCACGCCCTCACGGATCGCGTTCTGTTCGACGGCAAGCGCGCCAt       >  2:22832/1‑149 (MQ=255)                                                                                                                                          ttCGGTCCGATGGACCGCACCGTGACCAAGAACGGCCGCCGCTCCAGCACTGCCCGTGGTTACCTGGACCAGGCCAAGAAGCGCCCGAACCTGACCATCGTCACCCACGCCCTCACGGATCGCGTTCTGTTCGACGGCAAGCGCGCCAt       >  8:84990/1‑149 (MQ=255)                                                                                                                                             ggTCCGATGGACCGCACCGTGACCAAGAACGGCCGCCGCTCCAGCACTGCCCGTGGTTACCTGGACCAGGCCAAGAAGCGCCCGAACCTGACCATCGTCACCCACGCCCTCACGGATCGCGTTCTGTTCGACGGCAAGCGCGCCATCg     <  5:144000/148‑1 (MQ=255)                                                                                                                                               tCCGATGGACCGCACCGTGACCAAGAACGGCCGCCGCTCCAGCACTGCCCGTGGTTACCTGGACCAGGCCAAGAAGCGCCCGAACCTGACCATCGTCACCCACGCCCTCACGGATCGCGTTCTGTTCGACGGCAAGCGCGcc         <  2:25392/142‑1 (MQ=255)                                                                                                                                               tCCGATGGACCGCACCGTGACCAAGAACGGCCGCCGCTCCAGCACTGCCCGTGGTTACCTGGACCAGGCCAAGAAGCGCCCGAACCTGACCATCGTCACCCACGCCCTCACGGATCGCGTTCTGTTCGACGGCAAGCGCGCCATCGGCg  >  4:14420/1‑149 (MQ=255)                                                                                                                                               tCCGATGGACCGCACCGTGACCAAGAACAGCCGCCGCTCCAGCACTGCCCGTGGTTACCTGGACCAGGCCAAGAAGCGCCCGAACCTGACCATCGTCACCCACGCCCTCACGGATCGCGTTCTGTTCGACGGCAAGCGCGcc         >  1:25392/1‑142 (MQ=255)                                                                                                                                                   |                                                                                                                                                 CCATGGCGGCGAAGGCCCGGTCAGCGTGACCACGCCGAAAGCCGGCAACAACCCGCTGTTCCATGCCATGGTCGAAGCTGGCGTACAGGCCGGTTACCCACGCACAG‑AAGACCTCAACGGTTACCAGCAGGAAGGCTTCGGTCCGATGGACCGCACCGTGACCAAGAACGGCCGCCGCTCCAGCACTGCCCGTGGTTACCTGGACCAGGCCAAGAAGCGCCCGAACCTGACCATCGTCACCCACGCCCTCACGGATCGCGTTCTGTTCGACGGCAAGCGCGCCATCGGCG  >  NC_002947/5776103‑5776392

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑