Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,824,712 T→G 6.2% intergenic (+136/+38) PP_5102 → / ← trmB hypothetical protein/tRNA (guanosine(46)‑N7)‑methyltransferase TrmB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,824,7120TG 6.2% 232.7 / 2.7 76intergenic (+136/+38)PP_5102/trmBhypothetical protein/tRNA (guanosine(46)‑N7)‑methyltransferase TrmB
Reads supporting (aligned to +/- strand):  ref base T (36/35);  new base G (3/2);  total (39/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCGCGGCTGAGCGAATGACCCCTTGTAGGAGCGGATTCAACCGCGAAAGGGCCGGCCCTGCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTGGTGATCGGGCGCTCTTCGGGGCGCGGCACATAGGTGCCG  >  NC_002947/5824567‑5824858
                                                                                                                                                 |                                                                                                                                                  
ccgcGGCTGAGCGAATGACCCCTTGTAGGAGCGGATTCAACCGCGAAAGGGCCGGCCCTGCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGGAAACCCGCTCCTACAAAGAAAAAACCGCCCtttt                                                                                                                                                  >  8:62349/1‑148 (MQ=255)
ccgcGGCTGAGCGAATGACCCCTTGTAGGAGCGGATTCAACCGCGAAAGGGCCGGCCCTGCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCCACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCtttt                                                                                                                                                  >  8:363588/1‑148 (MQ=255)
     gCTGAGCGAATGACCCCTTGTAGGAGCGGATTCAACCGCGAAAGGGCCGGCCCTGCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGc                                                                                                                                            <  4:136439/149‑1 (MQ=255)
           cGAATGACCCCTTGTAGGAGCGGATTCAACCGCGAAAGGGCCGGCCCTGCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTGAAAAGGGCGGtttt                                                                                                                                      <  8:228895/149‑1 (MQ=255)
            gAATGACCCCTTGTAGGAGCGGATTCAACCGCGAAAGGGCCGGCCCTGCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTa                                                                                                                                     <  8:1266/149‑1 (MQ=255)
              aTGACCCCTTGTAGGAGCGGATTCAACCGCGAAAGGGCCGGCCCTGCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTAcc                                                                                                                                   <  3:359591/149‑1 (MQ=255)
               tGACCCCTTGTAGGAGCGGATTCAACCGCGAAAGGGCCGGCCCTGCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTAcc                                                                                                                                   >  6:213695/1‑148 (MQ=255)
                 aCCCCTTGTAGGAGCGGATTCAACCGCGAAAGGGCCGGCCCTGCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGa                                                                                                                                 >  7:217545/1‑148 (MQ=255)
                   cccTTGTAGGAGCGGATTCAACCGCGAAAGGGCCGGCCCTGCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGaaaa                                                                                                                              <  3:215418/149‑1 (MQ=255)
                     cTTGTAGGAGCGGATTCAACCGCGAAAGGGCCGGCCCTGCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTAcc                                                                                                                                   <  4:19220/142‑1 (MQ=255)
                     cTTGTAGGAGCGGATTCAACCGCGAAAGGGCCGGCCCTGCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTAcc                                                                                                                                   >  3:19220/1‑142 (MQ=255)
                     cTTGTAGGAGCGGATTCAACCGCGAAAGGGCCGGCCCTGCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAgg                                                                                                                            <  6:201202/149‑1 (MQ=255)
                           ggAGCGGATTCAACCGCGAAAGGGCCGGCCCTGCTGGCCAATCTCCA‑CCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTAt                                                                                                                      <  3:16070/148‑1 (MQ=255)
                              gCGGATTCAACCGCGAAAGGGCCGGCCCTGCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAg                                                                                                                   >  3:117797/1‑149 (MQ=255)
                                 gATTCAACCGCGAAAGGGCCGGCCCTGCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCg                                                                                                                >  5:44322/1‑149 (MQ=255)
                                 gATTCAACCGCGAAAGGGCCGGCCCTGCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCg                                                                                                                >  5:225217/1‑149 (MQ=255)
                                  aTTCAACCGCGAAAGGGCCGGCCCTGCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTAc                                                                                                                                    >  5:343415/1‑128 (MQ=255)
                                  aTTCAACCGCGAAAGGGCCGGCCCTGCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTAc                                                                                                                                    <  6:343415/128‑1 (MQ=255)
                                   ttCAACCGCGAAAGGGCCGGCCCTGCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTAGAAGCCGc                                                                                                               >  3:203483/1‑148 (MQ=255)
                                      aaCCGCGAAAGGGCCGGCCCTGCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCaa                                                                                                           >  3:289213/1‑149 (MQ=255)
                                      aaCCGCGAAAGGGCCGGCCCTGCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCaa                                                                                                           >  5:321463/1‑149 (MQ=255)
                                      aaCCGCGAAAGGGCCGGCCCTGCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCaa                                                                                                           >  1:260840/1‑149 (MQ=255)
                                      aaCCGCGAAAGGGCCGGCCCTGCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTGAAAAGGGCGGttttttcttt                                                                                                                                >  7:281774/1‑122 (MQ=18)
                                      aaCCGCGAAAGGGCCGGCCCTGCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTGAAAAGGGCGGttttttcttt                                                                                                                                <  8:281774/128‑7 (MQ=18)
                                       aCCGCGAAAGGGCCGGCCCTGCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAAt                                                                                                          >  3:176345/1‑149 (MQ=255)
                                       aCCGCGAAAGGGCCGGCCCTGCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAAt                                                                                                          >  7:9519/1‑149 (MQ=255)
                                       aCCGCGAAAGGGCCGGCCCTGCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAAt                                                                                                          <  5:235292/149‑1 (MQ=255)
                                         cgcgAAAGGGCCGGCCCTGCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGGTCCTACAAAGAAAAAACCGCCCTTTTCAGTGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATcc                                                                                                        <  1:189477/149‑1 (MQ=255)
                                            gAAAGGGCCGGCCCTGCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCAcc                                                                                                     <  5:390339/149‑1 (MQ=255)
                                                  gccggccCTGCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTtctc                                                                                                >  5:221003/1‑148 (MQ=255)
                                                     ggccCTGCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAAc                                                                                            <  4:117797/149‑1 (MQ=255)
                                                     ggccCTGCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAAc                                                                                            >  1:152122/1‑149 (MQ=255)
                                                      gccCTGCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACt                                                                                           >  4:381341/1‑149 (MQ=255)
                                                            ctgGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCaa                                                                                       <  4:289213/147‑1 (MQ=255)
                                                               gCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTccc                                                                                  >  8:72136/1‑149 (MQ=255)
                                                               gCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTcc                                                                                   >  7:217449/1‑148 (MQ=255)
                                                               gCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTc                                                                                    <  3:105883/147‑1 (MQ=255)
                                                               gCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTGTTCAGGGCGGTTTTACCGAAAAGGCCGTATCACCCGCTCAATCACCCTTCTCGAACGTCAAGTcc                                                                                   >  5:182415/1‑148 (MQ=37)
                                                                ccAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCca                                                                                 <  6:207382/149‑1 (MQ=255)
                                                                   aTCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCacac                                                                              <  8:217545/149‑1 (MQ=255)
                                                                       ccACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCg                                                                          >  2:69326/1‑149 (MQ=255)
                                                                        cACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGt                                                                         >  2:309345/1‑149 (MQ=255)
                                                                           ccGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGcc                                                                      >  2:68596/1‑149 (MQ=255)
                                                                             gCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTAACGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGcccc                                                                    >  8:142147/1‑149 (MQ=255)
                                                                              cccTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCa                                                                   <  8:9519/149‑1 (MQ=255)
                                                                                cTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGc                                                                 <  6:221003/149‑1 (MQ=255)
                                                                                cTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGc                                                                 <  6:385430/149‑1 (MQ=255)
                                                                                   cACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCg                                                               <  6:321463/148‑1 (MQ=255)
                                                                                     ccGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTc                                                            <  8:217449/149‑1 (MQ=255)
                                                                                      cGTGGGCGCCGCTACCGTATTCGCGGCTAAACCCGCTCCTACAAATAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCACCCCCTCAATCCAACTTCACGAACTTCAACTCCCACACCCCGTGCCCCAGCCGCTCa                                                           >  1:165923/1‑149 (MQ=255)
                                                                                       gagggCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCca                                                                                 >  5:289053/3‑126 (MQ=255)
                                                                                       gTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCca                                                                                 <  6:289053/126‑1 (MQ=255)
                                                                                               cgcTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCTAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGt                                                  <  7:40497/149‑1 (MQ=255)
                                                                                               cgcTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGt                                                  >  7:189174/1‑149 (MQ=255)
                                                                                               cgcTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGt                                                  >  6:215805/1‑149 (MQ=255)
                                                                                                      gTATTCGCGGGTAAACCAGCTCCTACAAAGAAAAAACCGCCCTGTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACt                                           >  3:296386/1‑149 (MQ=255)
                                                                                                         ttCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTgg                                        <  6:225217/149‑1 (MQ=255)
                                                                                                          tCGCGGTTGAATCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCTTTCGAACTTgg                                        <  2:143181/148‑1 (MQ=255)
                                                                                                            gcgGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTGGTGa                                     >  3:295798/1‑149 (MQ=255)
                                                                                                              gggTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTGGTGATc                                   <  7:363588/149‑1 (MQ=255)
                                                                                                                   aaCCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTGGTGATCGGgc                               <  5:215805/148‑1 (MQ=255)
                                                                                                                               cAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTGGTGATCGGGCGCTCTTCGGGgcg                  >  6:84105/1‑149 (MQ=255)
                                                                                                                                aaaGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTGGTGATCGGGCGCTCTTCGGGgcgc                 >  3:284988/1‑149 (MQ=255)
                                                                                                                                aaaGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTGGTGATCGGGCGCTCTTCGGGgcgc                 >  4:343506/1‑149 (MQ=255)
                                                                                                                                aaaCAAAAAACCGGCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTGGTGATCGGGCGCTCTTCGGGgcgc                 <  4:203483/149‑1 (MQ=255)
                                                                                                                                 aaGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTGGTGATCGGGCGCTCTTCGGGGcgcg                >  7:50984/1‑149 (MQ=255)
                                                                                                                                  aGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTGGTGATCGGGCGCTCTTCGGGGCGCgg               <  7:62349/149‑1 (MQ=255)
                                                                                                                                  aGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCACCCGCTCACCGCGGCGTTCGAACTTGGTGATCGGGCGCTCTTCGGGGCGCgg               >  8:141888/1‑149 (MQ=255)
                                                                                                                                   gAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTGGTGATCGGGCGCTCTTCGGGGCGCGGc              <  3:267201/149‑1 (MQ=255)
                                                                                                                                     aaaaaCCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTGGTGATCGGGCGCTCTTCGGGGCGCGGcac            <  4:284988/149‑1 (MQ=255)
                                                                                                                                      aaaaCCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGc                                                                       <  3:339142/89‑1 (MQ=255)
                                                                                                                                      aaaaCCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGc                                                                       >  4:339142/1‑89 (MQ=255)
                                                                                                                                      aaaaCCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTGGTGATCGGGCGCTCTTCGGGGCGCGGcaca           >  6:381686/1‑149 (MQ=255)
                                                                                                                                       aaaCCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTGGTGATCGGGCGCTCTTCGGGGCGCGGCACAt          <  8:50984/149‑1 (MQ=255)
                                                                                                                                         aCCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTGGTGATCGGGCGCTCTTCGGGGCGCGGCACATAg        <  1:69326/149‑1 (MQ=255)
                                                                                                                                           cGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTGGTGATCGGGCGCTCTTCGGGGCGCGGCACATAGGt      >  8:338486/1‑149 (MQ=255)
                                                                                                                                              ccTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTGGTGATCGGGCGCTCTTCGGGGCGCGGCACATAGGTGcc   <  2:106960/149‑1 (MQ=255)
                                                                                                                                               cTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTGGTGATCGGGCGCTCTTCGGGGCGCGGCACATAGGTGCCg  >  1:54083/1‑149 (MQ=255)
                                                                                                                                                ttttCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTGGTGATCGGGCGCTCTTCGGGGCGCGGCACATAGGTGCCg  >  3:401687/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
CCGCGGCTGAGCGAATGACCCCTTGTAGGAGCGGATTCAACCGCGAAAGGGCCGGCCCTGCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTGGTGATCGGGCGCTCTTCGGGGCGCGGCACATAGGTGCCG  >  NC_002947/5824567‑5824858

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑