Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,860,125 G→C 5.6% A535G (GCC→GGC)  PP_5136 ← ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,860,1250GC 5.6% 234.8 / 3.8 71A535G (GCC→GGC) PP_5136ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base G (34/33);  new base C (2/2);  total (36/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.21e-01

TGCATGGGTAGGTTCCTTACAGGTCAGACGGTCGCGGCGCGTTGGCGCCAGGCTTGGGAGCGGCGCAGCAGGCCGCGCGAGGCTGCAGCCAGCAACAGTGAGGCGCCGGCACTGGTCAGCAGGATCAAGGTGGACATGGCGGCGGCACCGCCAACGTTGCCGGCATCGTCCATGTTCAGCACGGCAATGGCAGCCAGGAGGGTGTCGGGGCTGTACAGAAAGATCGCTGCCGACACAGTGGTCATCGCCGATACGAACAGGTAGCGGATGATGTCCAGCAGCGCCGGCAGGC  >  NC_002947/5859981‑5860272
                                                                                                                                                |                                                                                                                                                   
tgCATGGGTAGGTTCCTTACAGGTCAGACGGGCGCGGCGCGTTGGCGCCAGGCTTGGGAGCGGCGCAGCAGGCCGCGCGAGGCTGCAGCCAGCAACAGTGAGGCGCCGGCACTGGTCAGCAGGATCAAGGTGGACATGGCGGCGGCAcc                                                                                                                                                 <  5:31261/149‑1 (MQ=255)
  cATGGGTAGGTTCCTTACAGGTCAGACGGTCGCGGCGCGTTGGCGCCAGGCTTGGGAGCGGCGCAGCAGGTCGCGCGAGGCTGCAGCCAGCAACAGTGAGGCGCCGGCACTGGTCAGCAGGATCAAGGTGGACATGGCGGCGGCACCGc                                                                                                                                               >  3:383377/1‑149 (MQ=255)
     gggTAGGTTCCTTACAGGTCAGACGGTCGCGGCGCGTTGGCGCCAGGCTTGGGAGCGGCGCAGCAGGCCGCGCGAGGCTGCAGCCAGCAACAGTGAGGCGCCGGCACTGGTCAGCAGGATCAAGGTGGACATGGCGGCGGCACCGCCaa                                                                                                                                            >  2:127170/1‑149 (MQ=255)
          ggTTCCTTACAGGTCAGACGGTCGCGGCGCGTTGGCGCCAGGCTTGGGAGCGGCGCAGCAGGCCGCGCGAGGCTGCAGCCAGCAACAGTGAGGCGCCGGCACTGGTCAGCAGGATCAAGGTGGACATGGCGGCGGCACCGCCAACGtt                                                                                                                                        <  3:149718/148‑1 (MQ=255)
          ggTTCCTTACAGGTCAGACGGTCGCGGCGCGTTGGCGCCAGGCTTGGGAGCGGCGCAGCAGGCCGCGCGAGGCTGCAGCCAGCAACAGTGAGGCGCCGGCACTGGTCAGCAGGATCAAGGTGGACATGGCGGCGGCACCGCCAACGtt                                                                                                                                        <  2:15477/148‑1 (MQ=255)
          ggTTCCTTACAGGTCAGACGGTCGCGGCGCGTTGGCGCCAGGCTGGGGAGCGGCGCAGCAGGCCGCGCGAGGCTGCAGCCAGCAACAGTGAGGCGCCGGCACTGGTCAGCAGGATCAAGGTGGACATGGCGGCGGCACCGCCAACGtt                                                                                                                                        <  1:341243/148‑1 (MQ=255)
               cTTACAGGTCAGACGGTCGCGGCGCGTTGGCGCCAGGCTTGGGAGCGGCGCAGCAGGCCGCGCGAGGCTGCAGCCAGCAACAGTGAGGCGCCGGCACTGGTCAGCAGGATCAAGGTGGACATGGCGGCGGCACCGCCAACGTTGCCgg                                                                                                                                   >  8:71010/1‑148 (MQ=255)
                ttACAGGTCAGACGGTCGCGGCGCGTTGGCGCCAGGCTTGGGAGCGGCGCAGCAGGCCGCGCGAGGCTGCAGCCAGCAACAGTGAGGCGCCGGCACTGGTCAGCAGGATCAAGGTGGACATGGCGGCGGCACCGCCAACGTTGCCgg                                                                                                                                   >  2:324206/1‑147 (MQ=255)
                ttACAGGTCAGACGGTCGCGGCGCGTTGGCGCCAGGCTTGGGAGCGGCGCAGCAGGCCGCGCGAGGCTGCAGCCAGCAACAGTGAGGCGCCGGCACTGGTCAGCAGGATCAAGGTGGACATGGCGGCGGCACCGCCAACGTTGCCGGCa                                                                                                                                 >  6:12626/1‑149 (MQ=255)
                 tACAGGTCAGACGGTCGCGGCGCGTTGGCGCCAGGCTTGGGAGCGGCGCAGCAGGCCGCGCGAGGCTGCAGCCAGCAACAGTGAGGCGCCGGCACTGGTCAGCAGGATCAAGGTGGACATGGCGGCGGCACCGCCAACGTTGCCGGCAt                                                                                                                                >  2:122682/1‑149 (MQ=255)
                  aCAGGTCAGACGGTCGCGGCGCGTTGGCGCCAGGCTTGGGAGCGGCGCAGCAGGCCGCGCGAGGCTGCAGCCAGCAACAGTGAGGCGCCGGCACTGGTCAGCAGGATCAAGGTGGACATGGCGGCGGCACCGCCAACGTTGCCGGCATc                                                                                                                               >  2:376629/1‑149 (MQ=255)
                    aGGTCAGACGGTCGCGGCGCGTTGGCGCCAGGCTTGGGAGCGGCGCAGCAGGCCGCGCGAGGCTGCAGCCAGCAACAGTGAGGCGCCGGCACTGGTCAGCAGGATCAAGGTGGACATGGCGGCGGCACCGCCAACGTTGCCGGCATCGt                                                                                                                             <  3:244917/149‑1 (MQ=255)
                    aGGTCAGACGGTCGCGGCGCGTTGGCGCCAGGCTTGGGAGCGGCGCAGCAGGCCGCGCGAGGCTGCAGCCAGCAACAGTGAGGCGCCGGCACTGGTCAGCAGGATCAAGGTGGACATGGCGGCGGCACCGCCAACGTTGCCGGCATCGt                                                                                                                             <  1:376629/149‑1 (MQ=255)
                      gTCAGACGGTCGCGGCGCGTTGGCGCCAGGCTTGGGAGCGGCGCAGCAGGCCGCGCGAGGCTGCAGCCAGCAACAGTGAGGCGCCGGCACTGGTCAGCAGGATCAAGGTGGACATGGCGGTGCCGCCGCCatgtcca                                                                                                                                       >  6:43271/1‑131 (MQ=255)
                      gTCAGACGGTCGCGGCGCGTTGGCGCCAGGCTTGGGAGCGGCGCAGCAGGCCGCGCGAGGCTGCAGCCAGCAACAGTGAGGCGCCGGCACTGGTCAGCAGGATCAAGGTGGACATGGCGGTGCCGCCGCCatgtcca                                                                                                                                       <  5:43271/137‑7 (MQ=255)
                        cAGACGGTCGCGGCGCGTTGGCGCCAGGCTTGGGAGCGGCGCAGCAGGCCGCGCGAGGCTGCAGCCAGCAACAGTGAGGCGCCGGCACTGGTCAGCAGGATCAAGGTGGACATGGCGGCGGCACCGCCAACGTTGCCGGCATCGTCCAt                                                                                                                         >  2:303721/1‑149 (MQ=255)
                        cAGACGGTCGCGGCGCGTTGGCGCCAGGCTTGGGAGCGGCGCAGCAGGCCGCGCGAGGCTGCAGCCAGCAACAGTGAGGCGCCGGCACTGGTCAGCAGGATCAAGGTGGACATGGCGGCGGCACCGCCAACGTTGCCGGCATCGTCCAt                                                                                                                         >  1:406205/1‑149 (MQ=255)
                           aCGGTCGCGGCGCGTTGGCGCCAGGCTTGGGAGCGGCGCAGCAGGCCGCGCGAGGCTGCAGCCAGCAACAGTGAGGCGCCGGCACTGGTCAGCAGGATCAAGGTGGACATGGCGGCGGCACCGCCAACGTTGCCGGCATCGTCCATGtt                                                                                                                      >  7:292321/1‑149 (MQ=255)
                             ggTCGCGGCGCGTTGGCGCCAGGCTTGGGAGCGGCGCAGCAGGCCGCGCGAGGCTGCAGCCAGCAACAGTGAGGCGCCGGCACTGGTCAGCAGGATCAAGGTGGACATGGCGGTGCCGCCGCCat                                                                                                                                            <  4:269374/125‑2 (MQ=255)
                                      gcgTTGGCGCCAGGCTTGGGAGCGGCGCAGCAGGTCGCGCGAGGCTGCAGCCAGCAACAGTGAGGCGCCGGCACTGGTCAGCAGGATCAAGGTGGACATGGCGGCGGCACCGCCAACGTTGCCGGCATCGTCCATGTTCAGCACGGCaa                                                                                                           <  4:383377/149‑1 (MQ=255)
                                       cgTTGGCGCCAGGCTTGGGAGCGGCGCAGCAGGCCGCGCGAGGCTGCAGCCAGCAACAGTGAGGCGCCGGCACTGGTCAGCAGGATCAAGGTGGACATGGCGGCGGCACCGCCAACGTTGCCGGCATCGTCCATGTTCAGCACGGCAAt                                                                                                          <  1:122682/149‑1 (MQ=255)
                                         ttGGCGCCAGGCTTGGGAGCGGCGCAGCAGGCCGCGCGAGGCTGCAGCCAGCAACAGTGAGGCGCCGGCACTGGTCAGCAGGATCAAGGTGGACATGGCGGCGGCACCGCCAACGTTGCCGGCATCGTCCATGTTCAGCACGGCAATgg                                                                                                        >  5:212162/1‑149 (MQ=255)
                                            gcgcCAGGCTTGGGAGCGGCGCAGCAGGCCGCGCGAGGCTGCAGCCAGCAACAGTGAGGCGCCGGCACTGGTCAGCAGGATCAAGGTGGACATGGCGGCGGCACCGCCAACGTTGCCGGCATCGTCCATGTTCAGCACGGCAATGGCa                                                                                                      <  3:251851/148‑1 (MQ=255)
                                            gcgcCAGGCTTGGGAGCGGCGCAGCAGGCCGCGCGAGGCTGCAGCCAGCAACAGTGAGGCGCCGGCACTGGTCAGCAGGATCAAGGTGGACATGGCGGCGGCACCGCCAACGTTGCCGGCATCGTCCATGTTCAGCACGGCAATGGCAg                                                                                                     >  4:78078/1‑149 (MQ=255)
                                             cgcCAGGCTTGGGAGCGGCGCAGCAGGCCGCGCGAGGCTGCAGCCAGCAACAGTGAGGCGCCGGCACTGGTCAGCAGGATCAAGGTGGACATGGCGGCGGCACCGCCAACGTTGCCGGCATCGTCCATGTTCAGCACGGCAATGGCAGc                                                                                                    >  3:341194/1‑149 (MQ=255)
                                              gcCAGGCTTGGGAGCGGCGCAGCAGGCCGCGCGAGGCTGCAGCCAGCAACAGTGAGGCGCCGGCACTGGTCAGCAGGATCAAGGTGGACATGGCGGCGGCACCGCCAACGTTGCCGGCATCGTCCATGTTCAGCACGGCAATGGCAGc                                                                                                    >  2:354313/1‑148 (MQ=255)
                                                   gCTTGGGAGCGGCGCAGCAGGCCGCGCGAGGCTGCAGCCAGCAACAGTGAGGCGCCGGCACTGGTCAGCAGGATCAAGGTGGACATGGCGGCGGCACCGCCAACGTTGCCGGCATCGTCCATGTTCAGCACGGCAATGGCAGCCaggag                                                                                              >  5:270928/1‑149 (MQ=255)
                                                   gCTTGGGAGCGGCGCAGCAGGCCGCGCGAGGCTGCAGCCAGCAACAGTGAGGCGCCGGCACTGGTCAGCAGGATCAAGGTGGACATGGCGGCGGCACCGCCAACGTTGCCGGCATCGTCCATGTTCAGCACGGCAATGGCAGCCaggag                                                                                              <  8:387416/149‑1 (MQ=255)
                                                   gCTTGGGAGCGGCGCAGCAGGCCGCGCGAGGCTGCAGCCAGCAACAGTGAGGCGCCGGCACTGGTCAGCAGGATCAAGGTGGACATGGCGGCGGCACCGCCAACGTTGCCGGCATCGTCCATGTTCAGCACGGCAATGGCAGCCaggag                                                                                              <  1:354313/149‑1 (MQ=255)
                                                    cTTGGGAGCGGCGCAGCAGGCCGCGCGAGGCTGCAGCCAGCAACAGTGAGGCGCCGGCACTGGTCAGCAGGATCAAGGTGGACATGGCGGCGGCACCGTCAACGTTGCCGGCATCGTCCATGTTCAGCACGGCAATGGCAGCCaggagg                                                                                             <  1:303721/149‑1 (MQ=255)
                                                    cTTGGGAGCGGCGCAGCAGGCCGCGCGAGGCTGCAGCCAGCAACAGTGAGGCGCCGGCACTGGTCAGCAGGATCAAGGTGGACATGGCGGCGGCACCGCCAACGTTGCCGGCATCGTCCATGTTCAGCACGGCAATGGCAGCCaggagg                                                                                             <  1:379182/149‑1 (MQ=255)
                                                    cTTGGGAGCGGCGCAGCAGGCCGCGCGAGGCTGCAGCCAGCAACAGTGAGGCGCCGGCACTGGTCAGCAGGATCAAGGTGGACATGGCGGCGGCACCGCCAACGTTGCCGGCATCGTCCATGTTCAGCACGGCAATGGCAGCCaggagg                                                                                             <  1:324206/149‑1 (MQ=255)
                                                        ggAGCGGCGCAGCAGGCCGCGCGAGGCTGCAGCCAGCAACAGTGAGGCGCCGGCACTGGTCAGCAGGATCAAGGTGGACATGGCGGCGGCACCGCCAACGTTGCCGGCATCGTCCATGTTCAGCACGGCAATGGCAGCCAGGAGGgtgt                                                                                         >  7:363518/1‑149 (MQ=255)
                                                             ggcgCAGCTGGCCGCGCGTGGCTGCAGGCAGGATCAGTGAGGCGCCGTGACTGTTCAGCAGGATCAAGGTGGACATGGCGGCGGCACCGCCAACGTTGCCGGCATCGTCCATGTTGAGCACGTCAATGGCTGCCAGGAGGGTGTCgggt                                                                                    <  4:158729/149‑2 (MQ=255)
                                                                 cagcagGCCGCGCGAGGATGCAGCCAGCAACAGTGAGGCGCCGGCACTGGTCAGCAGGATCAAGGTGGACATGGCGGCGGCACCGCCAACGTTGCCGGCATCGTCCATGTTCAGCACGGCAATGGCAGCCAGGAGGGTGTCGGGGCTg                                                                                 >  6:99029/1‑148 (MQ=255)
                                                                        ccGCGCGAGGCTGCAGCCAGCAACAGTGAGGCGCCGGCACTGGTCAGCAGGATCAAGGTGGACATGGCGGCGGCACCGCCAACGTTGCCGGCATCGTCCATGTTCAGCACGGCAATGGCAGCCAGGAGGGTGTCGGGGCTGTACAGaaa                                                                         <  6:270928/149‑1 (MQ=255)
                                                                              gAGGCTGCAGCCAGCAACAGTGAGGCGCCGGCACTGGTCAGCAGGATCAAGGTGGACATGGCGGCGGCACCGCCAACGTTGCCGGCATCGTCCATGTTCAGCACGGCAATGGCAGCCAGGAGGGTGACGGGGCTGTACAGAAAGATCGc                                                                   >  7:42258/1‑149 (MQ=255)
                                                                              gAGGCTGCAGCCAGCAACAGCGAGGCGCCGGCACTGGTCAGCAGGATCAAGGTGGACATGGCGGCGGCACCGCCAACGTTGCCGGCATCGTCCATGTTCAGCACGGCAATGGCAGCCAGGAGGGTGTCgg                                                                                      >  7:98406/1‑130 (MQ=255)
                                                                              gAGGCTGCAGCCAGCAACAGCGAGGCGCCGGCACTGGTCAGCAGGATCAAGGTGGACATGGCGGCGGCACCGCCAACGTTGCCGGCATCGTCCATGTTCAGCACGGCAATGGCAGCCAGGAGGGTGTCgg                                                                                      <  8:98406/130‑1 (MQ=255)
                                                                                 gCTGCAGCCAGCAACAGTGAGGCGCCGGCACTGGTCAGCAGGATCAAGGTGGACATGGCGGCGGCACCGCCAACGTTGCCGGCATCGTCCATGTTCAGCACGTCAATGGCAGCCAGGAGGGTGTCGGGGCTGTACAGAAAGATCGCTGc                                                                <  1:279786/149‑1 (MQ=255)
                                                                                     cagccagcAACAGTGAGGCGCCGGCACTGGTCAGCAGGATCAAGGTGGACATGGCGGCGGCACCGCCAACGTTGCCGGCATCGTCCATGTTCAGCACGGCAATGGCAGCCAGGAGGGTGTCGGGGCTGTACAGAAAGATCg                                                                    >  3:218538/1‑141 (MQ=255)
                                                                                     cagccagcAACAGTGAGGCGCCGGCACTGGTCAGCAGGATCAAGGTGGACATGGCGGCGGCACCGCCAACGTTGCCGGCATCGTCCATGTTCAGCACGGCAATGGCAGCCAGGAGGGTGTCGGGGCTGTACAGAAAGATCg                                                                    <  4:218538/141‑1 (MQ=255)
                                                                                     cagccagcAACAGTGAGGCGCCGGCACTGGTCAGCAGGATCAAGGTGGACATGGCGGCGGCACCGCCAACGTTGCCGGCATCGTCCATGTTCAGCACGGCAATGGCAGCCAGGAGGGTGTCGGGGCTGTACAGAAAGATCGCTGCCg                                                              <  6:212162/147‑1 (MQ=255)
                                                                                        ccagcAACAGTGAGGCGCCGGCACTGGTCAGCAGGATCAAGGTGGACATGGCGGCGGCACCGCCAACGTTGCCGGCATCGTCCATGTTCAGCACGGCAATGGCAGCCAGGAGGGTGTCGGGGCTGTACAGAAAGATCGCTGCCGaca                                                           >  6:40912/1‑147 (MQ=255)
                                                                                          agcAACAGTGAGGCGCCGGCACTGGTCAGCAGGATCAAGGTGGACATGGCGGCGGCACCGCCAACGTTGCCGGCATCGTcc                                                                                                                           <  7:31672/81‑1 (MQ=255)
                                                                                          agcAACAGTGAGGCGCCGGCACTGGTCAGCAGGATCAAGGTGGACATGGCGGCGGCACCGCCAACGTTGCCGGCATCGTcc                                                                                                                           >  8:31672/1‑81 (MQ=255)
                                                                                           gcAACAGTGAGGCGCCGGCACTGGTCAGCAGGATCAAGGTGGACATGGCGGCGGCACCGCCAACGTTGCCGGCATCGTCCATGTTCAGCACGGCAATGGCAGCCAGGAGGGTGTCGGGGCTGTACAGAAAGCTCGCTGCCGACACAGTg                                                      <  8:292321/149‑1 (MQ=255)
                                                                                            cAACAGTGAGGCGCCGGCACTGGTCAGCAGGATCAAGGTGGACATGGCGGCGGCACCGCCAACGTTGCCGGCATCGTCCATGTTCAGCACGGCAATGGCAGCCAGGAGGGTGTCGGGGCTGTACAGAAAGATCGCTGCCGACACAGTgg                                                     <  4:341194/149‑1 (MQ=255)
                                                                                             aaCAGTGAGGCGCCGGCACTGGTCAGCAGGATCAAGGTGGACATGGCGGCGGCACCACCAACGTTGCCGGCATCGTCCATGTTCAGCACGGCAATGGCAGCCAGGAGGGTGTCGGGGCTGTACAGAAAGATCGCTGCCGACACAGTg                                                      >  7:221346/1‑147 (MQ=255)
                                                                                             aaCAGTGAGGCGCCGGCACTGGTCAGCAGGATCAAGGTGGACATGGCGGCGGCACCACCAACGTTGCCGGCATCGTCCATGTTCAGCACGGCAATGGCAGCCAGGAGGGTGTCGGGGCTGTACAGAAAGATCGCTGCCGACACAGTg                                                      <  8:221346/147‑1 (MQ=255)
                                                                                                aGTGAGGCGCCGGCACTGGTCAGCAGGATCAAGGTGGACATGGCGGCGGCACCGCCAACGTTGCCGGCATCGTCCATGTTCAGCACGGCAATGGCAGCCAGGAGGGTGTCGGGGCTGTACAGAAAGATCGCTGCCGACACAGTGGTCAt                                                 >  3:12671/1‑149 (MQ=255)
                                                                                                aGTGAGGCGCCGGCACTGGTCAGCAGGATCAAGGTGGACATGGCGGCGGCACCGCCAACGTTGCCGGCATCGTCCATGTTCAGCACGGCAATGGCAGCCAGGAGGGTGTCGGGGCTGTACAGAAAGATCGCTGCCGACACAGTGGTCAt                                                 <  7:71010/149‑1 (MQ=255)
                                                                                                 gTGAGGCGCCGGCACTGGTCAGCAGGATCAAGGTGGACATGGCGGCGGCACCGCCAACGTTGCCGGCATCGTCCATGTTCAGCACGGCAATGGCAGCCAGGAGGGTGTCGGGGCTGTACAGAAAGATCGCTGCCGACACAGTGGTCATc                                                <  5:12626/149‑1 (MQ=255)
                                                                                                  tGAGGCGCCGGCACTGGTCAGCAGGATCAAGGTGGACATGGCGGCGGCACCGCCAACGTTGCCGGCATCGTCCATGTTCAGCACGGCAATGGCAGCCAGGAGGGTGTCGGGGCTGTACAGAAAGATCGCTGc                                                                <  8:357461/132‑1 (MQ=255)
                                                                                                  tGAGGCGCCGGCACTGGTCAGCAGGATCAAGGTGGACATGGCGGCGGCACCGCCAACGTTGCCGGCATCGTCCATGTTCAGCACGGCAATGGCAGCCAGGAGGGTGTCGGGGCTGTACAGAAAGATCGCTGc                                                                >  7:357461/1‑132 (MQ=255)
                                                                                                  tGAGGCGCCGGCACTGGTCAGCAGGATCAAGGTGGACATGGCGGCGGCACCGCCAACGTTGCCGGCATCGTCCATGTTCAGCACGGCAATGGCAGCCAGGAGGGTGTCGGGGCTGTACAGAAAGATCGCTGCCGACACAGTGGTCATCg                                               >  3:17866/1‑149 (MQ=255)
                                                                                                    aGGCGCCGGCACTGGTCAGCAGGATCAAGGTGGACATGGCGGCGGCACCGCCAACGTTGCCGGCATCGTCCATGTTCAGCACGGCAATGGCAGCCAGGAGGGTGTCGGGGCTGTACAGAAAGATCGCTGCCGACACAGTGGTCATCGcc                                             <  2:406205/149‑1 (MQ=255)
                                                                                                     ggCGCCGGCACTGGTCAGCAGGATCAAGGTGGACATGGCGGCGGCACCGCCAACGTTGCCGGCATCGTCCATGTTCAGCACGGCAATGGCAGCCAGGAGGGTGTCGGGGCTGTACAGAAAGATCGCTGCCGACACAGTGGTCATCGcc                                             >  7:170719/1‑148 (MQ=255)
                                                                                                            gCACTGGTCAGCAGGATCAAGGTGGACATGGCGGCGGCACCGCCAACGTTGCCGGCATCGTCCATGTTCAGCACGGCAATGGCAGCCAGGAGGGTGTCGGGGCTGTACAGAAAGATCGCTGCCGa                                                             <  7:299642/125‑1 (MQ=255)
                                                                                                              aCTGGTCAGCAGGATCAAGGTGGACATGGCGGCGGCACCGCCAACGTTGCCGGCATCGTCCATGTTCAGCACGGCAATGGCAGCCAGGAGGGTGTCGGGGCTGTACAGAAAGATCGCTGCCGACACAGTGGTCATCGCCGATACGAACa                                   <  3:78078/149‑1 (MQ=255)
                                                                                                                  gTCAGCAGGATCAAGGTGGACATGGCGGCGGCACCGCCAACGTTGCCGGCATCGTCCATGTTCAGCACGGCAATGGCAGACAGGAGGGTGTCGGGGCTGTACAGAAAGATCGCTGCCGACACAGTGGTCATCGCCGATACGAACAgg                                 >  4:322146/1‑147 (MQ=255)
                                                                                                                        agGATCAAGGTGGACATGGCGGCGGCACCGCCAACGTTGCCGGCATCGTCCATGTTCAGCACGGCAATGGCAGCCAGGAGGGTGTCGGGGCTGTACAGAAAGATCGCTGCCGACACAGTGGTCATCGCCGATACGAACAGGTAGCGgat                         <  5:40912/149‑1 (MQ=255)
                                                                                                                          gATCAAGGTGGACATGGCGGCGGCACCGCCAACGTTGCCGGCATCGTCCATGTTCAGCACGGCAATGGCAGCCAGGAGGGTGTCGGGGCTGTACAGAAAGATCGCTGCCGACACAGTGGTCATCGCCGATACGAACAGGTCGCGgatga                       <  5:99029/149‑1 (MQ=255)
                                                                                                                          gATCAAGGTGGACATGGCGGCGGCACCGCCAACGTTGCCGGCATCGTCCATGTTCAGCACGGCAATGGCAGCCAGGAGGGTGTCGGGGCTGTACAGAAAGATCGCTGCCGACACAGTGGTCATCGCCGATACGAACAGGTAGCGgatga                       >  5:32980/1‑149 (MQ=255)
                                                                                                                              aaGGTGGACATGGCGGCGGCACCGCCAACGTTGCCGGCATCGTCCATGTTCAGCACGGCAATGGCAGCCAGGAGGGTGTCGGGGCTGTACAGAAAGATCGCTGCCGACACAGTGGTCATCGCCGATACGAACAGGTAGCGGATGATGTc                   >  8:365510/1‑149 (MQ=255)
                                                                                                                              aaGGTGGACATGGCGGCGGCACCGCCAACGTTGCCGGCATCGTCCATGTTCAGCACGGCAATGGCAGCCAGGAGGGTGTCGGGGCTGTACAGAAAGATCGCTGCCGACACAGTGGTCATCGCCGATACGAACAGGTAGCGGATGATGTc                   >  5:412583/1‑149 (MQ=255)
                                                                                                                               aGGTGGACATGGCGGCGGCCCCGCCAACGTTGCCGGCAGCGTCCATGTTCAGCACGGCAATGGCAGCCAGGAGGGTGTCGGGGCTGTACAGAAAGATCGCTGCCGACACAGTGGTCATCGCCGATACGAACAGGTAGCGGATGATGTcc                  >  8:316207/1‑149 (MQ=255)
                                                                                                                               aGGTGGACATGGCGGCGGCACCGCCAACGTTGCCGGCATCGTCCATGTTCAGCACGGCAATGGCAGCCAGGAGGGTGTCGGGGCTg                                                                                 >  6:91184/1‑86 (MQ=255)
                                                                                                                               aGGTGGACATGGCGGCGGCACCGCCAACGTTGCCGGCATCGTCCATGTTCAGCACGGCAATGGCAGCCAGGAGGGTGTCGGGGCTg                                                                                 <  5:91184/86‑1 (MQ=255)
                                                                                                                                 gcaacgttGGCGGTGCCGCCGCCAACGTTGCCGGCATCGTCCATGTTCAGCACGGCAATGGCAGCCAGGAGGGTGTCGGGGCTGTACAGAAAGATCGCTGCCGACACAGTGGTCATCGCCGATACGAACAGGTAGCGGATGATGTCcag                >  7:191059/8‑149 (MQ=255)
                                                                                                                                       aTGGCGGCGGCACCGCCAACGTTGCCGGCATCGTCCATGTTCAGCACGGCAATGGCAGCCAGGAGGGTGTCGGGGCTGTACAGAAAGATCGCTGCCGACACAGTGGTCATCGCCGATACGAACAGGTAGCGGATGATGTCCAGCAgcgc          >  3:88183/1‑149 (MQ=255)
                                                                                                                                                gcACCGCCAACGTTGCCGGCATCGTCCATGTTCAGCACGGCAATGGCAGCCAGGAGGGTGTCGGGGCTGTACAGAAAGATCGCTGCCGACACAGTGGTCATCGCCGATACGAACAGGTAGCGGATGATGTCCAGCAGCGCCggcaggc  >  3:187007/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                                   
TGCATGGGTAGGTTCCTTACAGGTCAGACGGTCGCGGCGCGTTGGCGCCAGGCTTGGGAGCGGCGCAGCAGGCCGCGCGAGGCTGCAGCCAGCAACAGTGAGGCGCCGGCACTGGTCAGCAGGATCAAGGTGGACATGGCGGCGGCACCGCCAACGTTGCCGGCATCGTCCATGTTCAGCACGGCAATGGCAGCCAGGAGGGTGTCGGGGCTGTACAGAAAGATCGCTGCCGACACAGTGGTCATCGCCGATACGAACAGGTAGCGGATGATGTCCAGCAGCGCCGGCAGGC  >  NC_002947/5859981‑5860272

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑