Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,897,770	A→G	11.9%	intergenic (‑170/+64)	PP_5172 ← / ← PP_5173	hypothetical protein/RND family transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,897,770	0	A	G	11.9%	102.8 / 6.2	42	intergenic (‑170/+64)	PP_5172/PP_5173	hypothetical protein/RND family transporter
Reads supporting (aligned to +/- strand):  ref base A (14/23);  new base G (3/2);  total (17/25)
Fisher's exact test for biased strand distribution p-value = 3.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.94e-01

GGCGCCGGGCAGAGGTTTGAAGGGGTTTTGCGCCTGGCTTGAGGGCAATAGTAGTGAGGCGGGATTATTCTTAAAAATACTGTTTAAGAATTTTTATATAACCATTTAAGAGAACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATACGGTGGTCGATGCACCATCGCATTCGCGGGTAAACCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGT  >  NC_002947/5897622‑5897911                                                                                                                                                     |                                                                                                                                              ggCGCCGGGCAGAGGTTTGAAGGGGTTTTGCGCCTGGCTTGAGGGCAATAGTAGTGAGGCGGGATTATTCTTAAAAATACTGTTTAAGAATTTTTATATAACCATTTAAGAGAACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATa                                                                                                                                               >  5:414452/1‑149 (MQ=255)    gcCGGTCAGAGGTTTGAAGGGGTGTTGCGCCTGGCTTGAGGGCAATAGTAGTGAGGCGGGATTATTCTTAAAAATACTGTTTAAGAATTTTAATATAACCATTTAAGAGAACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATACgg                                                                                                                                            <  5:181196/149‑1 (MQ=255)      gcgcaagagGTTTGAAGGGGTTTTGCGCCTGGCTTGAGGGCAATAGTAGTGAGGCGGGATTATTCTTAAAAATACTGTTTAAGAATTTTTATATAACCATTTAAGAGAACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATACggtg                                                                                                                                          <  3:101727/144‑1 (MQ=255)      cGGGCAGAGGTTTGAAGGGGTTTTGCGCCTGGCTTGAGGGCAATAGTAGTGAGGCGGGATTATTCTTAAAAATACTGTTTAAGAATTTTTATATAACCATTTAAGAGAACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATACggtg                                                                                                                                          <  5:55351/149‑1 (MQ=255)       gggCAGAGGTTTGAAGGGGTTTTGCGCCTGGCTTGAGGGCAATAGTAGTGAGGCGGGATTATTCTTAAAAATACTGTTTAAGAATTTTTATATAACCATTTAAGAGAACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATACggtgg                                                                                                                                         <  3:331138/149‑1 (MQ=255)           agagGTTTGAAGGGGTTTTGCGCCTGGCTTGAGGGCAATAGTAGTGAGGCGGGATTATTCTTAAAAATACTGTTTAAGAATTTTTATATAACCATTTAAGAGAACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATACGGTGGTCGa                                                                                                                                     >  1:98536/1‑149 (MQ=255)            gagGTTTGAAGGGGTTTTGCGCCTGGCTTGAGGGCAATAGTAGTGAGGCGGGATTATTCTTAAAAATACTGTTTAAGAATTTTTATATAACCATTTAAGAGAACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATACGGTGGTCGAt                                                                                                                                    <  5:206952/149‑1 (MQ=255)            gagGTTTGAAGGGGTTTTGCGCCTGGCTTGAGGGCAATAGTAGTGAGGCGGGATTATTCTTAAAAATACTGTTTAAGAATTTTTATATAACCATTTAAGAGAACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATACGGTGGTCGAt                                                                                                                                    <  6:414452/149‑1 (MQ=255)             agGTTTGAAGGGGTTTTGCGCCTGGCTTGAGGGCAATAGTAGTGAGGCGGGATTATTCTTAAAAATACTGTTTAAGAATTTTTATATAACCATTTAAGAGAACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATACGGTGGTCGATg                                                                                                                                   <  2:146303/149‑1 (MQ=255)               gtgtGAAGGGGTTTTGCGCCTGGCTTGAGGGCAATAGTAGTGAGGCGGGATTATTCTTAAAAATACTGTTTAAGAATTTTTATATAACCATTTAAGAGAACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATACGGTGGTCGATGCa                                                                                                                                 <  5:86151/146‑1 (MQ=255)               gTTTGAAGGGGTTTTGCGCCTGGCTTGAGGGCAATAGTAGTGAGGCGGGATTATTCTTAAAAATACTGTTTAAGAATTTTTATATAACCATTTAAGAGAACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATACGGTGGTCGATGCa                                                                                                                                 >  1:224766/1‑149 (MQ=255)                    aaGGGGTTTTGCGCCTGGCTTGAGGGCAATAGTAGTGAGGCGGGATTATTCTTAAAAATACTGTTTAAGAATTTTTATATAACCATTTAAGAGAACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATACGGTGGTCGATGCACCAt                                                                                                                             <  6:144243/148‑1 (MQ=255)                             tGCGCCTGGCTTGAGGGCAATAGTAGTGAGGCGGGATTATTCTTAAAAATACTGTTTAAGAATTTTTATATAACCATTTAAGAGACCACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATACGGTGGTCGATGCACCATCGCATTcgc                                                                                                                    <  6:397261/148‑1 (MQ=255)                               cgcCTGGCTTGAGGGCAATAGTAGTGAGGCGGGATTATTCTTAAAAATACTGTTTAAGAATTTTTATATAACCATTTAAGAGAACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATACGGTGGTCGATGCACCATCGCATTCGCgg                                                                                                                  <  5:175455/148‑1 (MQ=255)                               cgcCTGGCTTGAGGGCAATAGTAGTGAGGCGGGATTATTCTTAAAAATACTGTTTAAGAATTTTTATATAACCATTTAAGAGAACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATACGGTGGTCGATGCACCATCGCATTCGCgg                                                                                                                  <  2:171683/148‑1 (MQ=255)                               cgcCTGGCTTGAGGGCAATAGTAGTGAGGCGGGATTATTCTTAAAAATACTGTTTAAGAATTTTTATATAACCATTTAAGAGAACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATACGGTGGTCGATGCACCATCGCATTCGCgg                                                                                                                  <  1:37461/148‑1 (MQ=255)                                  cTGGCTTGAGGGCAATAGTAGTGAGGCGGGATTATTCTTAAAAATACTGTTTAAGAATTTTTATATAACCATTTAAGAGAACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATGCGATGGTGCATCGACCACCGTATTCGCgg                                                                                                                  <  3:139398/145‑1 (MQ=25)                                  cTGGCTTGAGGGCAATAGTAGTGAGGCGGGATTATTCTTAAAAATACTGTTTAAGAATTTTTATATAACCATTTAAGAGAACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATGCGATGGTGCATCGACCACCGTATTCGCgg                                                                                                                  >  4:139398/1‑145 (MQ=25)                                  cTGGCTTGAGGGCAATAGTAGTGAGGCGGGATTATTCTTAAAAATACTGTTTAAGAATTTTTATATAACCATTTAAGAGAACACGAGTCTGGTAGGAGCGGGTTTACACGCGAATACGGTGGTCGATGCAGAATCGCATACGCGGGtc                                                                                                               >  5:8205/1‑147 (MQ=255)                                     tcTTGAGGGCAATTGTAGTTTGGCGGGATTATTCTTAAAAATACTGTTTAATAATTTTTATATACCCATTTAAGAGAACTCGCCTCTGGTAGGAGCGGGTTTACCGGCGAATACGGTGGTCGATGAACCATCGCATTCGCGGGTAAAcc                                                                                                           <  6:8205/148‑1 (MQ=38)                                     gCTTGAGGGCAATAGTAGTGAGGCGGGATTATTCTTAAAAATACTGTTTAAGAATTTTTATATAACCATTTAAGAGAACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATACGGTGGTCGATGCACCATCGCATTCGCGGGTAAAcc                                                                                                           <  2:98536/149‑1 (MQ=255)                                     gCTTGAGGGCAATAGTAGTGAGGCGGGATTATTCTTAAAAATACTGTTTAAGAATTTTTATATAACCATTTAAGAGAACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATACGGTGGTCGATGCACCATCGCATTCGCGGGTAAAc                                                                                                            <  8:70244/148‑1 (MQ=255)                                     gCTTGAGGGCAATAGTAGTGAGGCGGGATTATTCTTAAAAATACTGTTTAAGAATTTTTATATAACCATTTAAGAGAACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATACGGTGGTCGATGCACCATCGCATTCGCGGGTAAAc                                                                                                            <  2:143889/148‑1 (MQ=255)                                      cTTGAGGGCAATAGTAGTGAGGCGGGATTATTCTTAAAAATACTGTTTAAGAATTTTTATATAACCATTTAAGAGAACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATACGGTGGTCGATGCACCATCGCATTCGCGGGTAAAccc                                                                                                          <  8:166878/149‑1 (MQ=255)                                               aaTAGTAGTGAGGCGGGATTATTCTTAAAAATACTGTTTAAGAATTTTTATATAACCATTTAAGAGAACACGCCCCTGGTAGGAGCGGGTTTACCCGCGAATGCGATGGTGCATCGACCACCGTATTCGCGGGTAAAcc                                                                                                           <  5:194467/139‑1 (MQ=21)                                               aaTAGTAGTGAGGCGGGATTATTCTTAAAAATACTGTTTAAGAATTTTTATATAACCATTTAAGAGAACACGCCCCTGGTAGGAGCGGGTTTACCCGCGAATGCGATGGTGCATCGACCACCGTATTCGCGGGTAAAcc                                                                                                           >  6:194467/1‑139 (MQ=21)                                                                    ttCTTAAAAATACTTTTTAAGAATTTTAATATAACCATTAAAGAGAACACGCCTCTGGTAGGAGCGGGTTAACCCGCTAATACGGTGGTCGATGCACCATCGCATTCGCGGGTAAACCCGCTCCTACCAGGGGCCATATGGGCCTTCAg                                                                            <  2:224766/149‑1 (MQ=38)                                                                       ttAAAAATACTGTTTAAGAATTTTTATATAACCATTTAAGAGAACACGCCTCTGGTAGGAGCGGGTTTAACCGCGAATACGGTGGTCGATGCACCATAGCATTAGCGGGTAAAACCGCTCCTACCAGGGGCCATATTGGCCTTCAGGCg                                                                         >  3:238294/1‑149 (MQ=255)                                                                                        gAATTTTTATATAACCATTTAAGAGAACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATACGGTGGTGGATTCACCATCGCATTAGCGGGTAAACCCGCTCCTACCAGGGGCCATATGGGCCTTGAGGCGTGTTTCTGCGGCTcggg                                                        >  1:216865/1‑147 (MQ=255)                                                                                                     aCCATTTAAGAGAACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATACGGTGGTCGATGCACCATCGCAATCGCGGGTAAACCCGCTCCTAACAGGGGCCATATTGGCCTTCAAGCGGTTTTCTTCGGCTCGCCGATCATGTAACaa                                           >  5:6671/1‑148 (MQ=37)                                                                                                             atataaCAGGCGTTTGGTTGGAGGGTGTTTACCCGCTAATAGGGTGGTCGATGCACCATCGCATTCGCGGTTAAACCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTa                                   <  6:6671/145‑1 (MQ=255)                                                                                                             agagAACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATACGGTGGTCGATGCACCATCGCATTCGCGGGTAAACCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTa                                   >  3:46386/1‑149 (MQ=255)                                                                                                              gagaACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATACGGTGGTCGATGCACCATCGCATTCGCGGGTAAACCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTa                                   >  1:80452/1‑148 (MQ=255)                                                                                                                          ctGGTAGGAGCGGGTTTACCCGCGAATACGGTGGTCGATGCACCATCGCATTCGCGGGTAAACCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAgg                      >  1:236380/1‑149 (MQ=255)                                                                                                                             gTAGGAGCGGGTTTACCCGCGAATACGGTGGTCGATGCACCATCGCATTCGCGGGTAAACCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGaa                    <  6:378118/148‑1 (MQ=255)                                                                                                                                ggAGCGGGTTTACCCGCGAATGCGATGGTGCATCGACCACCGTATTCGCGGGTAAACCCGCACCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCAGGCAGGAAcag                 >  5:300331/1‑148 (MQ=21)                                                                                                                                   gCGGGTTTACCCGCGAATACGGTGGTCGATGCACCATCGCATTCGCGGGTAAACCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACagcagc             >  3:126484/1‑149 (MQ=255)                                                                                                                                       gTTTACCCGCGAATACGGTGGTCGATGCACCATCGCATTCGCGGGTAAACCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAAcag                 <  4:395340/141‑1 (MQ=255)                                                                                                                                       gTTTACCCGCGAATACGGTGGTCGATGCACCATCGCATTCGCGGGTAAACCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAAcag                 >  3:395340/1‑141 (MQ=255)                                                                                                                                       gTTTACCCGCGAATACGGTGGTCGATGCACCATCGCATTCGCGGGTAAACCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGt           >  4:61366/1‑147 (MQ=255)                                                                                                                                          tACCCGCGAATACGGTGGTCGATGCACCATCGCATTCGCGGGTAAACCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGc       <  4:238294/148‑1 (MQ=255)                                                                                                                                             ccGCGAATACGGTGGTCGATGCACCATCGCATTTGCGGGTAAACCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAgg   >  4:329386/1‑149 (MQ=255)                                                                                                                                              cgcgAATACGGTGGTCGATGCACCATCGCATTCGCGGGTAAACCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGACACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGt  >  2:317082/1‑149 (MQ=255)                                                                                                                                                     |                                                                                                                                              GGCGCCGGGCAGAGGTTTGAAGGGGTTTTGCGCCTGGCTTGAGGGCAATAGTAGTGAGGCGGGATTATTCTTAAAAATACTGTTTAAGAATTTTTATATAACCATTTAAGAGAACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATACGGTGGTCGATGCACCATCGCATTCGCGGGTAAACCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGT  >  NC_002947/5897622‑5897911

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑