Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,897,778	C→G	14.9%	intergenic (‑178/+56)	PP_5172 ← / ← PP_5173	hypothetical protein/RND family transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,897,778	0	C	G	14.9%	93.9 / 9.3	40	intergenic (‑178/+56)	PP_5172/PP_5173	hypothetical protein/RND family transporter
Reads supporting (aligned to +/- strand):  ref base C (13/21);  new base G (4/2);  total (17/23)
Fisher's exact test for biased strand distribution p-value = 3.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.53e-01

AGAGGTTTGAAGGGGTTTTGCGCCTGGCTTGAGGGCAATAGTAGTGAGGCGGGATTATTCTTAAAAATACTGTTTAAGAATTTTTATATAACCATTTAAGAGAACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATACGGTGGTCGATGCACCATCGCATTCGCGGGTAAACCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGA  >  NC_002947/5897632‑5897922                                                                                                                                                   |                                                                                                                                                 agagGTTTGAAGGGGTTTTGCGCCTGGCTTGAGGGCAATAGTAGTGAGGCGGGATTATTCTTAAAAATACTGTTTAAGAATTTTTATATAACCATTTAAGAGAACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATACGGTGGTCGa                                                                                                                                                >  1:98536/1‑149 (MQ=255)  gagGTTTGAAGGGGTTTTGCGCCTGGCTTGAGGGCAATAGTAGTGAGGCGGGATTATTCTTAAAAATACTGTTTAAGAATTTTTATATAACCATTTAAGAGAACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATACGGTGGTCGAt                                                                                                                                               <  5:206952/149‑1 (MQ=255)  gagGTTTGAAGGGGTTTTGCGCCTGGCTTGAGGGCAATAGTAGTGAGGCGGGATTATTCTTAAAAATACTGTTTAAGAATTTTTATATAACCATTTAAGAGAACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATACGGTGGTCGAt                                                                                                                                               <  6:414452/149‑1 (MQ=255)   agGTTTGAAGGGGTTTTGCGCCTGGCTTGAGGGCAATAGTAGTGAGGCGGGATTATTCTTAAAAATACTGTTTAAGAATTTTTATATAACCATTTAAGAGAACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATACGGTGGTCGATg                                                                                                                                              <  2:146303/149‑1 (MQ=255)     gtgtGAAGGGGTTTTGCGCCTGGCTTGAGGGCAATAGTAGTGAGGCGGGATTATTCTTAAAAATACTGTTTAAGAATTTTTATATAACCATTTAAGAGAACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATACGGTGGTCGATGCa                                                                                                                                            <  5:86151/146‑1 (MQ=255)     gTTTGAAGGGGTTTTGCGCCTGGCTTGAGGGCAATAGTAGTGAGGCGGGATTATTCTTAAAAATACTGTTTAAGAATTTTTATATAACCATTTAAGAGAACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATACGGTGGTCGATGCa                                                                                                                                            >  1:224766/1‑149 (MQ=255)          aaGGGGTTTTGCGCCTGGCTTGAGGGCAATAGTAGTGAGGCGGGATTATTCTTAAAAATACTGTTTAAGAATTTTTATATAACCATTTAAGAGAACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATACGGTGGTCGATGCACCAt                                                                                                                                        <  6:144243/148‑1 (MQ=255)                   tGCGCCTGGCTTGAGGGCAATAGTAGTGAGGCGGGATTATTCTTAAAAATACTGTTTAAGAATTTTTATATAACCATTTAAGAGACCACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATACGGTGGTCGATGCACCATCGCATTcgc                                                                                                                               <  6:397261/148‑1 (MQ=255)                     cgcCTGGCTTGAGGGCAATAGTAGTGAGGCGGGATTATTCTTAAAAATACTGTTTAAGAATTTTTATATAACCATTTAAGAGAACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATACGGTGGTCGATGCACCATCGCATTCGCgg                                                                                                                             <  2:171683/148‑1 (MQ=255)                     cgcCTGGCTTGAGGGCAATAGTAGTGAGGCGGGATTATTCTTAAAAATACTGTTTAAGAATTTTTATATAACCATTTAAGAGAACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATACGGTGGTCGATGCACCATCGCATTCGCgg                                                                                                                             <  5:175455/148‑1 (MQ=255)                     cgcCTGGCTTGAGGGCAATAGTAGTGAGGCGGGATTATTCTTAAAAATACTGTTTAAGAATTTTTATATAACCATTTAAGAGAACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATACGGTGGTCGATGCACCATCGCATTCGCgg                                                                                                                             <  1:37461/148‑1 (MQ=255)                        cTGGCTTGAGGGCAATAGTAGTGAGGCGGGATTATTCTTAAAAATACTGTTTAAGAATTTTTATATAACCATTTAAGAGAACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATGCGATGGTGCATCGACCACCGTATTCGCgg                                                                                                                             <  3:139398/145‑1 (MQ=25)                        cTGGCTTGAGGGCAATAGTAGTGAGGCGGGATTATTCTTAAAAATACTGTTTAAGAATTTTTATATAACCATTTAAGAGAACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATGCGATGGTGCATCGACCACCGTATTCGCgg                                                                                                                             >  4:139398/1‑145 (MQ=25)                        cTGGCTTGAGGGCAATAGTAGTGAGGCGGGATTATTCTTAAAAATACTGTTTAAGAATTTTTATATAACCATTTAAGAGAACACGAGTCTGGTAGGAGCGGGTTTACACGCGAATACGGTGGTCGATGCAGAATCGCATACGCGGGtc                                                                                                                          >  5:8205/1‑147 (MQ=255)                           tcTTGAGGGCAATTGTAGTTTGGCGGGATTATTCTTAAAAATACTGTTTAATAATTTTTATATACCCATTTAAGAGAACTCGCCTCTGGTAGGAGCGGGTTTACCGGCGAATACGGTGGTCGATGAACCATCGCATTCGCGGGTAAAcc                                                                                                                      <  6:8205/148‑1 (MQ=38)                           gCTTGAGGGCAATAGTAGTGAGGCGGGATTATTCTTAAAAATACTGTTTAAGAATTTTTATATAACCATTTAAGAGAACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATACGGTGGTCGATGCACCATCGCATTCGCGGGTAAAcc                                                                                                                      <  2:98536/149‑1 (MQ=255)                           gCTTGAGGGCAATAGTAGTGAGGCGGGATTATTCTTAAAAATACTGTTTAAGAATTTTTATATAACCATTTAAGAGAACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATACGGTGGTCGATGCACCATCGCATTCGCGGGTAAAc                                                                                                                       <  8:70244/148‑1 (MQ=255)                           gCTTGAGGGCAATAGTAGTGAGGCGGGATTATTCTTAAAAATACTGTTTAAGAATTTTTATATAACCATTTAAGAGAACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATACGGTGGTCGATGCACCATCGCATTCGCGGGTAAAc                                                                                                                       <  2:143889/148‑1 (MQ=255)                            cTTGAGGGCAATAGTAGTGAGGCGGGATTATTCTTAAAAATACTGTTTAAGAATTTTTATATAACCATTTAAGAGAACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATACGGTGGTCGATGCACCATCGCATTCGCGGGTAAAccc                                                                                                                     <  8:166878/149‑1 (MQ=255)                                     aaTAGTAGTGAGGCGGGATTATTCTTAAAAATACTGTTTAAGAATTTTTATATAACCATTTAAGAGAACACGCCCCTGGTAGGAGCGGGTTTACCCGCGAATGCGATGGTGCATCGACCACCGTATTCGCGGGTAAAcc                                                                                                                      >  6:194467/1‑139 (MQ=21)                                     aaTAGTAGTGAGGCGGGATTATTCTTAAAAATACTGTTTAAGAATTTTTATATAACCATTTAAGAGAACACGCCCCTGGTAGGAGCGGGTTTACCCGCGAATGCGATGGTGCATCGACCACCGTATTCGCGGGTAAAcc                                                                                                                      <  5:194467/139‑1 (MQ=21)                                                          ttCTTAAAAATACTTTTTAAGAATTTTAATATAACCATTAAAGAGAACACGCCTCTGGTAGGAGCGGGTTAACCCGCTAATACGGTGGTCGATGCACCATCGCATTCGCGGGTAAACCCGCTCCTACCAGGGGCCATATGGGCCTTCAg                                                                                       <  2:224766/149‑1 (MQ=38)                                                             ttAAAAATACTGTTTAAGAATTTTTATATAACCATTTAAGAGAACACGCCTCTGGTAGGAGCGGGTTTAACCGCGAATACGGTGGTCGATGCACCATAGCATTAGCGGGTAAAACCGCTCCTACCAGGGGCCATATTGGCCTTCAGGCg                                                                                    >  3:238294/1‑149 (MQ=255)                                                                              gAATTTTTATATAACCATTTAAGAGAACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATACGGTGGTGGATTCACCATCGCATTAGCGGGTAAACCCGCTCCTACCAGGGGCCATATGGGCCTTGAGGCGTGTTTCTGCGGCTcggg                                                                   >  1:216865/1‑147 (MQ=255)                                                                                           aCCATTTAAGAGAACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATACGGTGGTCGATGCACCATCGCAATCGCGGGTAAACCCGCTCCTAACAGGGGCCATATTGGCCTTCAAGCGGTTTTCTTCGGCTCGCCGATCATGTAACaa                                                      >  5:6671/1‑148 (MQ=37)                                                                                                   atataaCAGGCGTTTGGTTGGAGGGTGTTTACCCGCTAATAGGGTGGTCGATGCACCATCGCATTCGCGGTTAAACCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTa                                              <  6:6671/145‑1 (MQ=255)                                                                                                   agagAACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATACGGTGGTCGATGCACCATCGCATTCGCGGGTAAACCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTa                                              >  3:46386/1‑149 (MQ=255)                                                                                                    gagaACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATACGGTGGTCGATGCACCATCGCATTCGCGGGTAAACCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTa                                              >  1:80452/1‑148 (MQ=255)                                                                                                                ctGGTAGGAGCGGGTTTACCCGCGAATACGGTGGTCGATGCACCATCGCATTCGCGGGTAAACCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAgg                                 >  1:236380/1‑149 (MQ=255)                                                                                                                   gTAGGAGCGGGTTTACCCGCGAATACGGTGGTCGATGCACCATCGCATTCGCGGGTAAACCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGaa                               <  6:378118/148‑1 (MQ=255)                                                                                                                      ggAGCGGGTTTACCCGCGAATGCGATGGTGCATCGACCACCGTATTCGCGGGTAAACCCGCACCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCAGGCAGGAAcag                            >  5:300331/1‑148 (MQ=21)                                                                                                                         gCGGGTTTACCCGCGAATACGGTGGTCGATGCACCATCGCATTCGCGGGTAAACCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACagcagc                        >  3:126484/1‑149 (MQ=255)                                                                                                                             gTTTACCCGCGAATACGGTGGTCGATGCACCATCGCATTCGCGGGTAAACCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAAcag                            <  4:395340/141‑1 (MQ=255)                                                                                                                             gTTTACCCGCGAATACGGTGGTCGATGCACCATCGCATTCGCGGGTAAACCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAAcag                            >  3:395340/1‑141 (MQ=255)                                                                                                                             gTTTACCCGCGAATACGGTGGTCGATGCACCATCGCATTCGCGGGTAAACCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGt                      >  4:61366/1‑147 (MQ=255)                                                                                                                                tACCCGCGAATACGGTGGTCGATGCACCATCGCATTCGCGGGTAAACCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGc                  <  4:238294/148‑1 (MQ=255)                                                                                                                                   ccGCGAATACGGTGGTCGATGCACCATCGCATTTGCGGGTAAACCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAgg              >  4:329386/1‑149 (MQ=255)                                                                                                                                    cgcgAATACGGTGGTCGATGCACCATCGCATTCGCGGGTAAACCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGACACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGt             >  2:317082/1‑149 (MQ=255)                                                                                                                                            cGGTGGTCGATGCACCATCGCATTCGCGGGTAAACCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACga     <  3:61366/149‑1 (MQ=255)                                                                                                                                               tggtCGATGCACCATCGCATTCGCGGGTAAACCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACgatga  <  2:236380/149‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                 AGAGGTTTGAAGGGGTTTTGCGCCTGGCTTGAGGGCAATAGTAGTGAGGCGGGATTATTCTTAAAAATACTGTTTAAGAATTTTTATATAACCATTTAAGAGAACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATACGGTGGTCGATGCACCATCGCATTCGCGGGTAAACCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGA  >  NC_002947/5897632‑5897922

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑