Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,925,591	C→A	5.8%	R185L (CGC→CTC)	gcvT‑II ←	aminomethyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,925,591	0	C	A	5.8%	278.6 / 4.5	85	R185L (CGC→CTC)	gcvT‑II	aminomethyltransferase
Reads supporting (aligned to +/- strand):  ref base C (39/41);  new base A (2/3);  total (41/44)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.50e-01

AGGTTCATGCCGGCTTCCAGGCGCAGGGTATCGCGGGCGCCCAGGCCGCTTGGGGCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGATGAGTGCAGCGCGCGCGGGGCTGAGCAGGGCCGCCACCTTTTCGCGGGCACGCGGCCCCTGGATGGCAAGGATGGCCAGATCAGGGCGGACCTG  >  NC_002947/5925444‑5925739                                                                                                                                                      |                                                                                                                                                     aGGTTCATGCCGGCTTCCAGGCGCAGGGTATCGCGGGCGCCCAGGCCGCTTGGGGCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCg                                                                                                                                                     <  4:125846/149‑1 (MQ=255)  ggTTCATGCCGGCTTCCAGGCGCAGGGTATCGCGGGCGCCCAGGCCGCTTGGGGCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCgg                                                                                                                                                    <  2:270230/149‑1 (MQ=255)   gTTCATGCCGGCTTCCAGGCGCAGGGTATCGCGGGCGCCCAGGCCGCTTGGGGCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCggg                                                                                                                                                   >  5:298909/1‑149 (MQ=255)   gTTCATGCCGGCTTCCAGGCGCAGGGTATCGCGGGCGCCCAGGCCGCTTGGGGCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCggg                                                                                                                                                   <  1:25578/149‑1 (MQ=255)   gTTCATGCCGGCTTCCAGGCGCAGGGTATCGCGGGCGCCCAGGCCGCTTGGGGCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCggg                                                                                                                                                   <  5:319323/149‑1 (MQ=255)   gTTCATGCCGGCTTCCAGGCGCAGGGTATCGCGGGCGCCCAGGCCGCTTGGGGCGATACCGGCGCCGACAAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCggg                                                                                                                                                   >  6:3216/1‑149 (MQ=255)     tCATGCCGGCTTCCAGGCGCAGGGTATCGCGGGCGCCCAGGCCGCTTGGGGCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGGCTATA‑CCGGTGAggacg                                                                                                                                                 >  8:20449/1‑146 (MQ=255)     tCATGCCGGCTTCCAGGCGCAGGGTATCGCGGGCGCCCAGGCCGCTTGGGGCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGGCTATA‑CCGGTGAggacg                                                                                                                                                 >  8:14562/1‑146 (MQ=255)      cATGCCGGCTTCCAGGCGCAGGGTATCGCGGGCGCCCAGGCCGCTTGGGGCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGGCTATA‑CCGGTGAggacg                                                                                                                                                 <  7:20449/148‑4 (MQ=255)      cATGCCGGCTTCCAGGCGCAGGGTATCGCGGGCGCCCAGGCCGCTTGGGGCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGGCTATA‑CCGGTGAggacg                                                                                                                                                 <  7:14562/148‑4 (MQ=255)        tGCCGGCTTCCAGGCGCAGGGTATCGCGGGCGCCCAGGCCGCTTGGGGCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAAt                                                                                                                                               >  7:92677/1‑148 (MQ=255)          ccGGCTTCCAGGCGCAGGGTATCGCTGGCGCCCAGGCCGCTTGGGGCGATACCGGCGCCGACCAGGGCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGa                                                                                                                                             <  3:326084/148‑1 (MQ=255)          ccGGCTTCCAGGCGCAGGGTATCGCGGGCGCCCAGGCCGCTTGGGGCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGaa                                                                                                                                            <  6:271400/149‑1 (MQ=255)          ccGGCTTCCAGGCGCAGGGTATCGCGGGCGCCCAGGCCGCTTGGGGCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGaa                                                                                                                                            >  6:265008/1‑149 (MQ=255)          ccGGCTTCCAGGCGCAGGGTATCGCGGGCGCCCAGGCCGCTTGGGGCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCGCACCGGCTATA‑CCGGTGAggacggcct                                                                                                                                             <  6:303472/148‑8 (MQ=255)          ccGGCTTCCAGGCACAGGGTATCGCGGGCGCCCAGGCCGCTTGGGGCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGaa                                                                                                                                            <  7:64748/149‑1 (MQ=255)             gCTTCCAGGCGCAGGGTATCGCGGGCGCCCAGGCCGCTTGGGGCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAAc                                                                                                                                           >  1:402054/1‑147 (MQ=255)               ttCCAGGCGCAGGGAATCGCGGGCGCCCAGGCCGCTTGGGGCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATATC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCACTGAACAAGt                                                                                                                                       >  7:346743/1‑149 (MQ=255)                     gcgcAGGGTATCGCGGGCGCCCAGGCCGCTTGGGGCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGt                                                                                                                                 <  6:35780/149‑1 (MQ=255)                       gcAGGGTATCGCGGGCGCCCAGGCCGCTTGGGGCGATACCGGCTCCGACCAGGTCGTTGAAGAAGGCCACCTCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGTCAATGAACCAGTCGCCGTCg                                                                                                                               <  4:132315/149‑1 (MQ=255)                         aGGGTATCGCGGGCGCCCAGGCCGCTTGGGGCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGc                                                                                                                             <  5:183449/149‑1 (MQ=255)                                 gcgGGCGCCCAGGCCGCTTGGGGCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCTGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGccc                                                                                                                      <  4:160827/148‑1 (MQ=255)                                 gcgGGCGCCCAGGCCGCTTGGGGCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCt                                                                                                                     >  6:188507/1‑149 (MQ=255)                                      cgcCCAGGCCGCTTGGGGCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAAc                                                                                                                <  5:265008/149‑1 (MQ=255)                                       gcCCAGGCCGCTTGGGGCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCATCCTCACCGG‑TATAGCCGGTGCGGGCAATGaa                                                                                                                                            >  4:300052/1‑120 (MQ=255)                                       gcCCAGGCCGCTTGGGGCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCATCCTCACCGG‑TATAGCCGGTGCGGGCAATGaa                                                                                                                                            <  3:300052/120‑1 (MQ=255)                                        cccAGGCCGCTTGGGGCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACgg                                                                                                              >  3:116625/1‑149 (MQ=255)                                             gCCGCTTGGGGCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCa                                                                                                         <  5:188507/149‑1 (MQ=255)                                             gCCGCTTGGGGCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCa                                                                                                         <  6:24260/149‑1 (MQ=255)                                             gCCGCTTGGGGCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCAGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGgcg                                                                                                           >  2:312432/1‑147 (MQ=255)                                                     gggCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTcgcg                                                                                                  <  4:185441/148‑1 (MQ=255)                                                      ggCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCg                                                                                                                                     <  7:167731/112‑1 (MQ=255)                                                      ggCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCg                                                                                                                                     >  8:167731/1‑112 (MQ=255)                                                      ggCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCggc                                                                                                <  8:363292/149‑2 (MQ=255)                                                      ggCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGa                                                                                                <  6:298909/149‑1 (MQ=255)                                                       gCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTCGGAGGCCGTCATAACCGG‑TTAACCGGGTGCGGGCAATGAACAAGTCGCCGACGGAAACCCCCACCAACGGGCGCCGTTCCCGGa                                                                                                >  1:325526/1‑149 (MQ=255)                                                       gCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCACCGCCCTCGAACGGGCGCAGTTCGCGGAt                                                                                               >  8:230392/1‑149 (MQ=255)                                                       gCGATACCGGCGCCGACAAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGAt                                                                                               <  5:3216/149‑1 (MQ=255)                                                        cGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGGCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGAt                                                                                               >  1:25979/1‑148 (MQ=255)                                                         gATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGATGa                                                                                             <  2:283029/149‑1 (MQ=255)                                                              cGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAg                                                                                                                                        >  7:41882/1‑101 (MQ=255)                                                              cGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAg                                                                                                                                        <  8:41882/101‑1 (MQ=255)                                                                     gACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGATGAGTGCAgcgcgcg                                                                                 >  3:333769/1‑149 (MQ=255)                                                                               ttGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGATGAGTGCAGCGCGCGCGGGGCTGAg                                                                       >  5:276877/1‑149 (MQ=255)                                                                                 gaagaaGGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGATGAGTGCAGCGCGCGCGGGGCTGAg                                                                       >  3:153671/1‑147 (MQ=255)                                                                                 gaagaaGGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGATGAGTGCAGCGCGCGCGGGGCTGAg                                                                       >  3:202206/1‑147 (MQ=255)                                                                                   agaagGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGATGAGTGCAGCGCGCGCGGGGCTGAGCAgg                                                                   <  2:25979/149‑1 (MQ=255)                                                                                     aagGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGGCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGATGAGTGCAGCGCGCGCGGGGCTGAGCAGGgc                                                                 <  7:230392/149‑1 (MQ=255)                                                                                         ccACCGCCTGATCGCCCTGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGATGAGTGCAGCGCGCGCGGGGCTGAGCAGGgccgcc                                                             <  1:288979/149‑1 (MQ=255)                                                                                          cACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGATGAGTGCAGCGCGCGCGGGGCTGAGCAGGGCCGCCa                                                            >  2:312430/1‑149 (MQ=255)                                                                                           aCCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGATGAGTGCAGCGCGCGCGGGGCTGAGCAGGGCCGCCAc                                                           >  3:200609/1‑149 (MQ=255)                                                                                           aCCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCAAACGGGCGCAGTTCGCGGATGAGTGCAGCGCGCGCGGGGCTGAGCAGGGCCGCCAc                                                           >  6:117817/1‑149 (MQ=255)                                                                                                  gATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGATGAGTGCAGCGCGCGCGGGGCTGAGCAGGGCCGCCACCTTTTcg                                                    >  7:300192/1‑149 (MQ=255)                                                                                                   aTCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGATGAGTGCAGCGCGCGCGGGGCTGAGCAGGGCCGCCACCTTTTcgc                                                   <  2:14150/149‑1 (MQ=255)                                                                                                   aTCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGATGAGTGCAGCGCGCGCGGGGCTGAGCAGGGCCGCCACCTTTTcgc                                                   <  4:153671/149‑1 (MQ=255)                                                                                                    tCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGATGAGTGCAGCGCGCGCGGGGCTGAGCAGGGCCGCCACCTTTTcgcg                                                  <  1:239114/149‑1 (MQ=255)                                                                                                      gCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGATGAGTGCAGCGCGCGCGGGGCTGAGCAGGGCCGCCACCTTTTCGCgg                                                 <  1:312432/148‑1 (MQ=255)                                                                                                       cccGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGATGAGTGCAGCGCGCGCGGGGCTGAGCAGGGCCGCCACCTTTTCGCggg                                                >  1:114836/1‑148 (MQ=255)                                                                                                       cccGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGATGAGTGCAGCGCGCGCGGGGCGGAGCAGGGCCGCCACCTTTTCGCGGGc                                               >  7:294284/1‑149 (MQ=255)                                                                                                           ggAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGACCCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGATGAGTGCAGCGCGCGCGGGGCTGAGCAGGGCCGCCACCTTTTCGCGGGCAcgc                                           <  7:138065/149‑1 (MQ=255)                                                                                                              aGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGATGAGTGCAGCGCGCGCGGGGCTGAGCAGGgccgcc                                                             <  7:156423/128‑1 (MQ=255)                                                                                                              aGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGATGAGTGCAGCGCGCGCGGGGCTGAGCAGGgccgcc                                                             >  8:156423/1‑128 (MQ=255)                                                                                                               gatgatCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGATGAGTGCAGCGCGCGCGGGGCTGAGCAGGGCCGCCACCTTTTCGCGGGCCCGCGGcc                                       >  8:330884/1‑149 (MQ=255)                                                                                                                  gatCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGATGAGTGCAGCGCGCGCGGGGCTGAGCAGGGCCGCCACCTTTTCGCGGGCACGCGGCCCCt                                    <  8:294284/149‑1 (MQ=255)                                                                                                                      tc‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGATGAGTGCAGCGCGCGCGGGGCTGAGCAGGGCCGCCACCttt                                                       >  7:21133/1‑126 (MQ=255)                                                                                                                      tc‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGATGAGTGCAGCGCGCGCGGGGCTGAGCAGGGCCGCCACCttt                                                       <  8:21133/126‑1 (MQ=255)                                                                                                                       c‑cAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGATGAGTGCAGCGCGCGCGGGGCTGAGCAGGGCCGCCACCTTTTCGCGGGCACGCGGCCCCTGGATg                               >  4:54910/1‑149 (MQ=255)                                                                                                                       c‑cAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGATGAGTGCAGCGCGCGCGGGGCTGAGCAGGGCCGCCACCTTTTCGCGGGCACGCGGCCCCTGGATg                               >  1:363210/1‑149 (MQ=255)                                                                                                                         cAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGATGAGTGCAGCGCGCGCGGGGCTGAGCAGGGCCGCCACCTTTTCGCGGGCACGCGGCCCCTGGAt                                <  4:202206/147‑1 (MQ=255)                                                                                                                         cAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGATGAGTGCAGCGCGCGCGGGGCTGAGCAGGGCCGCCACCTTTTCGCGGGCACGCGGCCCCTGGATg                               <  4:333769/148‑1 (MQ=255)                                                                                                                         cAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGATGAGTGCAGCGCGCGCGGGGCTGAGCAGGGCCGCCACCTTTTCGCGGGCACGCGGCCCCTGGATg                               <  2:402054/148‑1 (MQ=255)                                                                                                                           ggCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGATGAGTGCAGCGCGCGCGGGGCTGAGCAGGGCCGCCACCTTTTCGCGGGCACGCGGCCCCTGGATGGc                             >  2:77776/1‑148 (MQ=255)                                                                                                                              cGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGATGAGTGCAGCGCGCGCGGGGCTGAGCAGGGCCGCCACCTTTTCGCGGGCACGCGGCCCCTGGATGGCAAg                          >  1:313552/1‑148 (MQ=255)                                                                                                                                   tCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACACCATCGAAAGGACGAAGTTCGGGGATGAGTGCAGCGCGCGCGGGGATGAGCAGGGCCGCAACCTTTTCGCGGGCACGCGGCCCCAGGATCGCAAGGATGGc                    >  8:255966/1‑149 (MQ=255)                                                                                                                                    cACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGATGAGTGCAGCGCGCGCGGGGCTGAGCAGGGCCGCCACCTTTTCGCGGGCACGCGGCCCCTGGATGGCAAGGATGGc                    >  5:59465/1‑148 (MQ=255)                                                                                                                                    cACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGATGAGTGCAGCGCGCGCGGGGCTGAGCAGGGCCGCCACCTTTTCGCGGGCACGCGGCCCCTGGATGGCAAGGATGGc                    >  3:340438/1‑148 (MQ=255)                                                                                                                                     aCCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGATGAGTGCAGCGCGCGCGGGGCTGAGCAGGGCCGCCACCTTTTCGCGGGCACGCGGCCCCTGGATGGCAAGGATGGc                    >  8:305138/1‑147 (MQ=255)                                                                                                                                      ccGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGATGAGTGCAGCGCGCGCGGGGCTGAGCAGGGCCGCCACCTTTTCGCGGGCACGCGGCCCCTGGATGGCAAGGATGGCCAg                 <  7:305138/149‑1 (MQ=255)                                                                                                                                         g‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGATGAGTGCAGCGCGCGCGGGGCTGAGCAGGGCCGCCACCTTTTCGCGGGCACGCGGCCCCTGGATGGCAAGGATGGCCAGAt               >  3:251042/1‑148 (MQ=255)                                                                                                                                           tataGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGATGAGTGCAGCGCGCGCGGGGCTGAGCAGGGCCGCCACCTTTTCGCGGGCACGCGGCCCCTGGATGGCAAGGATGGCCAGATCa             <  5:117817/149‑1 (MQ=255)                                                                                                                                             taGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGATGAGTGCAGCGCGCGCGGGGCTGAGCAGGGCCGCCACCTTTTCGCGGGCACGCGGCCCCTGGATGGCAAGGATGGCCAGATCAgg           >  2:356827/1‑149 (MQ=255)                                                                                                                                               ggcGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGATGAGTGCAGCGCGCGCGGGGCTGAGCAGGGCCGCCACCTTTTCGCGGGCACGCGGCCCCTGGATGGCAAGGATGGCCAGATCAggg          <  8:346743/146‑1 (MQ=255)                                                                                                                                                ccGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGATGAGTGCAGCGCGCGCGGGGCTGAGCAGGGCCGCCACCTTTTCGCGGGCACGCGGCCCCTGGATGGCAAGGATGGCCAGATCAGGGc         <  8:300192/148‑1 (MQ=255)                                                                                                                                                   gTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGATGAGTGCAGCGCGCGCGGGGCTGAGCAGGGCCGCCACCTTTTCGCGGGCACGCGGCCCCTGGATGGCAAGGATGGCCAGATCAGGGCGGAc     >  4:249488/1‑149 (MQ=255)                                                                                                                                                   gTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGATGAGTGCAGCGCGCGCGGGGCTGAGCAGGGCCGCCACCTTTTCGCGGGCACGCGGCCCCTGGATGGCAAGGATGGCCAGATCAGGGCGGAc     <  4:116625/149‑1 (MQ=255)                                                                                                                                                      cGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGATGAGTGCAGCGCGCGCGGGGCTGAGCAGGGCCGCCACCTTTTCGCGGGCACGCGGCCCCTGGATGGCAAGGATGGCCAGATCAGGGCGGACCTg  <  6:276877/149‑1 (MQ=255)                                                                                                                                                      |                                                                                                                                                     AGGTTCATGCCGGCTTCCAGGCGCAGGGTATCGCGGGCGCCCAGGCCGCTTGGGGCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGATGAGTGCAGCGCGCGCGGGGCTGAGCAGGGCCGCCACCTTTTCGCGGGCACGCGGCCCCTGGATGGCAAGGATGGCCAGATCAGGGCGGACCTG  >  NC_002947/5925444‑5925739

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑