BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,959,995	A→T	7.2%	noncoding (74/103 nt)	PP_mr63 ←	rnk_pseudo ncRNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,959,995	0	A	T	7.2%	362.8 / 12.7	124	noncoding (74/103 nt)	PP_mr63	rnk_pseudo ncRNA
Reads supporting (aligned to +/- strand): ref base A (62/53); new base T (5/4); total (67/57)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 125 total aligned reads displayed.

CTCTCGTCGAGGCTGTCGATCAGACGCTCCAGACGCTGTACGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAA‑‑‑‑‑CCCGA‑‑‑CACTAC‑CACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCGCGCAAGACCTGGGCCTCGCGGCAGATCTGCCGGCGCCGTTCGT  >  NC_002947/5959853‑5960143

ctctCGTCGAGGCTGTCGATCAGACGCTCCAGACGCTGTACGTCCAATCGGGTGAGGATTAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGc                                                                                                                                                          <  3:240669/148‑1 (MQ=255)

 tctcGTCGAGGCTGTCGATCAGACGCTCCAGACGCTGTACGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCTTGGGCgg                                                                                                                                                        >  3:141354/1‑149 (MQ=255)

     gtcgAGGCTGTCGATCAGACGCTCCAGACGCTGTACGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTTCAAATAAAGCAAAACCCCGCCCTTGGGCgggg                                                                                                                                                      <  4:141354/147‑1 (MQ=255)

     gtcgAGGCTGTCGATCAGACGCTCCAGACGCTGTACGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCggg                                                                                                                                                       <  5:33539/146‑1 (MQ=255)

     gtcgAGGCTGTCGATCAGACGCTCCAGACGCTGTACGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCggg                                                                                                                                                       >  6:33539/1‑146 (MQ=255)

     gtcgAGGCTGTCGATCAGACGCTCCAGACGCTGTACGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCggg                                                                                                                                                       <  7:28250/146‑1 (MQ=255)

     gtcgAGGCTGTCGATCAGACGCTCCAGACGCTGTACGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGt                                                                                                                                                     <  2:204369/148‑1 (MQ=255)

        gcggCTGCCGATCCGACGCTCCAGACGCTGTACGTCCAATCGGGTGCGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTga                                                                                                                                                 <  8:358230/147‑1 (MQ=255)

          ggCTGTCGATCAGACGCTCCAGACGCTGTACGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTgag                                                                                                                                                >  2:173636/1‑148 (MQ=255)

               tCGATCAGACGCTCCAGACGCTGTACGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAgg                                                                                                                                            <  4:174819/147‑1 (MQ=255)

                 gATCAGACGCTCCAGACGCTGTACGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCttt                                                                                                                                        <  2:269590/149‑1 (MQ=255)

                 gATCAGACGCTCCAGACGCTGTACGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCttt                                                                                                                                        >  6:287472/1‑149 (MQ=255)

                  aTCAGACGCTCCAGACGCTGTACGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTg                                                                                                                                       >  3:39669/1‑149 (MQ=255)

                      gACGCTCCAGACGCTGTACGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTgag                                                                                                                                                <  5:261637/136‑1 (MQ=255)

                      gACGCTCCAGACGCTGTACGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTgag                                                                                                                                                >  2:302868/1‑136 (MQ=255)

                      gACGCTCCAGACGCTGTACGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTgag                                                                                                                                                >  6:261637/1‑136 (MQ=255)

                      gACGCTCCAGACGCTGTACGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTgag                                                                                                                                                <  1:302868/136‑1 (MQ=255)

                       aCGCTCCAGACGCTGTACGTCCAATCGGGTGAGGATGAGGGAAGTCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACACAGCACAAGGGCGGGGTTTTAGAAGGCTTTGGCGt                                                                                                                                   >  6:58695/1‑148 (MQ=255)

                         gCTCCAGACGCTGTACGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAgg                                                                                                                                            <  6:7807/137‑1 (MQ=255)

                         gCTCCAGACGCTGTACGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAgg                                                                                                                                            >  5:7807/1‑137 (MQ=255)

                         gCTCCAGACGCTGTACGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCAc                                                                                                                                <  3:143726/149‑1 (MQ=255)

                           tCCAGACGCTGTACGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTCGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCAcc                                                                                                                               >  7:15049/1‑148 (MQ=255)

                             cAGACGCTGTACGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGa                                                                                                                             <  2:237704/148‑1 (MQ=255)

                             cAGACGCTGTACGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGa                                                                                                                             <  6:215613/148‑1 (MQ=255)

                             cAGACGCTGTACGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGAc                                                                                                                            >  5:267884/1‑149 (MQ=255)

                                   cTGTACGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCAc                                                                                                                                <  1:106557/139‑1 (MQ=255)

                                    tGTACGTCGAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAA‑‑‑‑‑c                                                                                                                >  2:295420/1‑149 (MQ=255)

                                        cGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAA‑‑‑‑‑CCCGa                                                                                                            <  2:63102/149‑1 (MQ=255)

                                        cGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGAAAAACCCCGCCCAAGGCCGGGGTTGCAGAAGGCTTTGGCTTCACCGACGCAGAA‑‑‑‑‑CCCg                                                                                                             >  1:351628/1‑148 (MQ=255)

                                         gTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAA‑‑‑‑‑CCCGa‑‑‑c                                                                                                        <  1:157255/149‑1 (MQ=255)

                                          tCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGttt                                                                                                                                                   >  6:126189/1‑113 (MQ=255)

                                          tCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGttt                                                                                                                                                   <  5:126189/113‑1 (MQ=255)

                                          tCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAA‑‑‑‑‑CCCGa‑‑‑ca                                                                                                       <  6:92797/149‑1 (MQ=255)

                                          tCCAATAGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCAGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTAACCGACGCAGAA‑‑‑‑‑CCCGa‑‑‑ca                                                                                                       >  6:181112/1‑149 (MQ=255)

                                             aaTCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCTTGGGCGGGGttttgct                                                                                                                                               >  8:241488/1‑110 (MQ=255)

                                             aaTCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAA‑‑‑‑‑CCCGA‑‑‑CACTa                                                                                                    <  5:287472/149‑1 (MQ=255)

                                              aTCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAA‑‑‑‑‑CCCGA‑‑‑CACTAc                                                                                                   <  7:80665/149‑1 (MQ=255)

                                              aTCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAA‑‑‑‑‑CCCGA‑‑‑CACTAc                                                                                                   >  5:156732/1‑149 (MQ=255)

                                                 gggTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAA‑‑‑‑‑CCCGA‑‑‑CACTAC‑ca                                                                                                >  7:241255/1‑148 (MQ=255)

                                                   gTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGt                                                                                                                                                     >  3:13857/1‑102 (MQ=255)

                                                   gTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGt                                                                                                                                                     <  4:13857/102‑1 (MQ=255)

                                                   gTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAA‑‑‑‑‑CCCGA‑‑‑CACTAC‑CACAg                                                                                             >  8:64535/1‑149 (MQ=255)

                                                        gATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGc                                                                                                                          <  5:138399/124‑1 (MQ=255)

                                                            aGGGAAGGCTTGTTGCTCATTATCCATTCAGACTCATTTGAACAGGCAGTTATCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGTTGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAA‑‑‑‑‑CCCGA‑‑‑CACTAC‑CACAGCCGGGCaa                                                                                     <  5:58695/148‑1 (MQ=255)

                                                               gAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAA‑‑‑‑‑CCCGA‑‑‑CACTAC‑CACAGCCGGGCAAATAc                                                                                 <  6:267884/149‑1 (MQ=255)

                                                                aaGGCTTGGTGCTCATGATCCATGCATACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGTCTTTGGCGTCACCGACGCAGAA‑‑‑‑‑CCCGA‑‑‑CACTAC‑CACAGCCGGGCAAATACt                                                                                <  1:99385/149‑1 (MQ=255)

                                                                     ttGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAA‑‑‑‑‑CCCGA‑‑‑CACTAC‑CCCAGCCGGGCAAATACTCAAGc                                                                           >  7:261360/1‑149 (MQ=255)

                                                                      tGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAA‑‑‑‑‑CCCGA‑‑‑CACTAC‑CACAGCCGGGCAAATACTCAAGcc                                                                          >  2:60054/1‑149 (MQ=255)

                                                                       ggTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAA‑‑‑‑‑CACGA‑‑‑CACTAC‑CACAGCCGGGCAAATACTCAAGcc                                                                          >  5:301481/1‑148 (MQ=255)

                                                                          gCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAA‑‑‑‑‑CCCGA‑‑‑CACTAC‑CACAGCCGGGCAAATACTc                                                                               >  5:143724/1‑140 (MQ=255)

                                                                          gCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAA‑‑‑‑‑CCCGA‑‑‑CACTAC‑CACAGCCGGGCAAATACTc                                                                               <  6:143724/140‑1 (MQ=255)

                                                                             cATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAA‑‑‑‑‑CCCGA‑‑‑CACTAC‑CACAGCCGGGCAAATACTCAAGCCCCTgcgc                                                                   >  3:249974/1‑149 (MQ=255)

                                                                             cATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAA‑‑‑‑‑CCCGA‑‑‑CACTAC‑CACAGCCGGGCAAATACTCAAGCCCCTgcgc                                                                   >  4:53983/1‑149 (MQ=255)

                                                                             cATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCAAAGGGCGGGGTTTGAGAAGTCTTTGGCGTCACAGACGCAGAA‑‑‑‑‑CCAGA‑‑‑CACTAC‑CACAGCCTGGCAAATACTCAAGCCCCTgcgc                                                                   >  5:337675/1‑149 (MQ=255)

                                                                              aTGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAA‑‑‑‑‑CCCGA‑‑‑CACTAC‑CCCAGCCGGGCAAATACTCAAGCCCCTGCGCt                                                                  >  2:72849/1‑149 (MQ=255)

                                                                                  tCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAgg                                                                                                                                            >  5:242163/1‑80 (MQ=255)

                                                                                  tCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAA‑‑‑‑‑CCCGA‑‑‑CACTAC‑CACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAgt                                                              <  8:57694/149‑1 (MQ=255)

                                                                                   ccAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAA‑‑‑‑‑CCCGA‑‑‑CACTAC‑CACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAgtg                                                             >  3:292966/1‑149 (MQ=255)

                                                                                    cAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAA‑‑‑‑‑CCCGA‑‑‑CACTAC‑CACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAgtgt                                                            <  6:156732/149‑1 (MQ=255)

                                                                                      gtcttaCTCTTTTGAAAAGGATTTTTTAAATTTGAAAAT‑AAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGGCACCGACGCATAA‑‑‑‑‑CCCGA‑‑‑CACTAC‑CACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGcc                                                         <  2:351628/144‑1 (MQ=255)

                                                                                           aCTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAA‑‑‑‑‑CCCGA‑‑‑CACTAC‑CACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAg                                                     >  1:313362/1‑149 (MQ=255)

                                                                                            ctacTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAA‑‑‑‑‑CCCGA‑‑‑CACTAC‑CACAGCAGGGCAAATACTCAAGACACTGCGCTCAGTGTGCCTTAg                                                     >  2:340908/4‑148 (MQ=255)

                                                                                            cTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAA‑‑‑‑‑CCCGA‑‑‑CACTAC‑CACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGc                                                    <  4:343445/149‑1 (MQ=255)

                                                                                            cTCCTTTGAACAGGCAGGTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAA‑‑‑‑‑CCCGA‑‑‑CACTAC‑CACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGc                                                    <  7:354931/149‑1 (MQ=255)

                                                                                               cTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAA‑‑‑‑‑CCCGA‑‑‑CACTAC‑CACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTg                                                 <  8:261360/149‑1 (MQ=255)

                                                                                               cTTCGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAA‑‑‑‑‑CCCGA‑‑‑CACTAC‑CACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTg                                                 <  8:15049/149‑1 (MQ=255)

                                                                                                tttGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGAGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAA‑‑‑‑‑CCCGA‑‑‑CACTAC‑CCCAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCtcc                                                >  8:239499/1‑147 (MQ=255)

                                                                                                  tGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAA‑‑‑‑‑CCCGA‑‑‑CACTAC‑CACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGc                                                <  1:173636/147‑1 (MQ=255)

                                                                                                       aGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAA‑‑‑‑‑CCCGA‑‑‑CACTAC‑CACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCgcgc                                          >  3:46879/1‑148 (MQ=255)

                                                                                                         gCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGC‑GGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAA‑‑‑‑‑CCCGA‑‑‑CACTAC‑CACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCGCGCAAGa                                      >  3:148707/1‑149 (MQ=255)

                                                                                                          cAGTTTTCAATGTGCAAATAAATCAAAACCCCGCCAAATGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAA‑‑‑‑‑CCCGA‑‑‑CACTAC‑CACACCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCGCGCAAGa                                      <  4:44080/149‑1 (MQ=255)

                                                                                                             ttttCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAA‑‑‑‑‑CCCGA‑‑‑CACTAC‑CACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCGCGCAAGACCt                                   >  3:122418/1‑149 (MQ=255)

                                                                                                              tttCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAA‑‑‑‑‑CCCGA‑‑‑CACTAC‑CACAGCCGGGCAAATACTCAAGCCCCTGCGCTCACTGTGCCTTAGCCTGCTCGCGCAAGACCTg                                  >  7:105496/1‑149 (MQ=255)

                                                                                                               ttCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAA‑‑‑‑‑CCCGA‑‑‑CACTAC‑CACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCGCGCAAGACCTgg                                 >  6:345025/1‑149 (MQ=255)

                                                                                                                 cAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAA‑‑‑‑‑CCCGA‑‑‑CACTAC‑CACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCGCGCAAGACCTGGGc                               >  3:278899/1‑149 (MQ=255)

                                                                                                                  aaTGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAA‑‑‑‑‑CCCGA‑‑‑CACTAC‑CACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCGCGCAAGACCTGGGcc                              <  5:42163/149‑1 (MQ=255)

                                                                                                                  aaTGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAA‑‑‑‑‑CCCGA‑‑‑CACTAC‑CACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCGCGCAAGACCTGGGcc                              <  7:97689/149‑1 (MQ=255)

                                                                                                                      tgCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAA‑‑‑‑‑CCCGA‑‑‑CACTAC‑CACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCGCGCAAGACCTGGGCCTcgc                          >  8:321904/1‑149 (MQ=255)

                                                                                                                      tgCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAA‑‑‑‑‑CCCGA‑‑‑CACTAC‑CACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCGCGCAAGACCTGGGCCTcg                           <  7:299395/148‑1 (MQ=255)

                                                                                                                        cAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAA‑‑‑‑‑CCCGA‑‑‑CACTAC‑CACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCGCGCAAGACCTGGGCCTcgcg                         <  4:387467/148‑1 (MQ=255)

                                                                                                                         aaaTAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAA‑‑‑‑‑CCCGA‑‑‑CACTAC‑CACAGCCGGGCAAATACTCAAGCCCCTCCGCTCAGTGTGCCTTAGCCTGCTCGCGCAAGACCTGGGCCTCGCGGc                       <  1:295420/149‑1 (MQ=255)

                                                                                                                          aaTAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAA‑‑‑‑‑CCCGA‑‑‑CACTAC‑CACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCGCGCAAGACCTGGGCCTCGCgg                        >  2:342004/1‑147 (MQ=255)

                                                                                                                          aaTAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAA‑‑‑‑‑CCCGA‑‑‑CACTAC‑CACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCGCGCAAGACCTGGGCCTCGCGGCa                      <  7:64535/149‑1 (MQ=255)

                                                                                                                               aGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAA‑‑‑‑‑CCCGA‑‑‑CACTAC‑CACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCGCGCAAGACCTGGGCCTCGCGGCAGATCt                 <  5:302214/149‑1 (MQ=255)

                                                                                                                               aGCAAAACCCAGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAA‑‑‑‑‑CCCGA‑‑‑CACTAC‑CACAGCCGGGCAAATACTCAAGCACCTGCGCTCAGTGTGCCTTAGCCTGCTCGCGCAAGACCTGGGACTCGAGGCAGATCt                 >  7:236754/1‑149 (MQ=255)

                                                                                                                                gCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAA‑‑‑‑‑CCCGA‑‑‑CACTAC‑CACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCGCGCAAGACCTGGGCCTCGCGGCAGATCTg                >  6:411784/1‑149 (MQ=255)

                                                                                                                                gCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAA‑‑‑‑‑CCCGA‑‑‑CACTAC‑CACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCGCGCAAGACCTGGGCCTCGCGGCAGATCTg                >  5:134361/1‑149 (MQ=255)

                                                                                                                                  aaaaCCCCGCCCATGGGCGGTTTTTGAGAAGTCTTTGGCGTCACCGTCGCATTACCTCTCCCTATCCCCCCACTCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCGCGCAAGACCTGGGCCTCGCgg                        <  1:334562/148‑1 (MQ=255)

                                                                                                                                    aaCCCCGCCCTTGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAA‑‑‑‑‑CCCGA‑‑‑CACTAC‑CACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCGCGCaa                                        >  7:211661/1‑121 (MQ=255)

                                                                                                                                      ccccGCCCTTGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAA‑‑‑‑‑CCCGAG‑‑CACTAC‑CACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCGCGCAAGACCTGGGCCTCGCGGCAGATCTGCCGgc           >  1:314843/1‑149 (MQ=255)

                                                                                                                                      ccccGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAA‑‑‑‑‑CCCGA‑‑‑CACTAC‑CACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCGCGCAAGACCTGGGCCTcgc                          >  1:374986/1‑133 (MQ=255)

                                                                                                                                      ccccGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAA‑‑‑‑‑CCCGA‑‑‑CACTAC‑CACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCGCGCAAGACCTGGGCCTcgc                          <  2:374986/133‑1 (MQ=255)

                                                                                                                                      ccccGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAA‑‑‑‑‑CCCGA‑‑‑CACTAC‑CACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCGCGCAAGACCTGGGCCTCGCGGCAGATCTGCCGgcg          >  2:214197/1‑149 (MQ=255)

                                                                                                                                       cccGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAA‑‑‑‑‑CCCGA‑‑‑CACTAC‑CACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCGCGCAAGACCTGGGCCTCGCGGCAGATCTGCCGgcgc         >  4:396365/1‑149 (MQ=255)

                                                                                                                                         cGCCCAAGGGGGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAA‑‑‑‑‑CCCGA‑‑‑CACTAC‑CACAGCCGTGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCGCGCAAGACCTGGGCCTCGCGGCAGATCTGCCGGCGCCg       <  6:337675/149‑1 (MQ=255)

                                                                                                                                         cGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAA‑‑‑‑‑CCCGA‑‑‑CACTAC‑CACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCGCGCAAGACCTGGGCCTCGCGGCAGATCTGCCGGCGCCg       <  1:22013/149‑1 (MQ=255)

                                                                                                                                         cGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAA‑‑‑‑‑CCCGA‑‑‑CACTAC‑CACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCGCGCAAGACCTGGGCCTCGCGGCAGATCTGCCGGCGCCg       <  6:301481/149‑1 (MQ=255)

                                                                                                                                          gCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAA‑‑‑‑‑CCCGA‑‑‑CACTAC‑CACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCGCGCAAGACCTGGGCCTCGCGGCAGATCTGCCGGCGCCGt      <  4:46879/149‑1 (MQ=255)

                                                                                                                                            ccAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAA‑‑‑‑‑CCCGA‑‑‑CACTAC‑CACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCGCGCAAGACCTGGGCCTCGCGGCAGATCTGCCGGCGCCGTTc    >  2:132039/1‑149 (MQ=255)

                                                                                                                                              aaGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAA‑‑‑‑‑CCCGA‑‑‑CACTAC‑CACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCGCGCAAGACCTGGGCCTCGCGACAGATCTGCCGGCGCCGTTCGt  <  8:241255/149‑1 (MQ=255)

CTCTCGTCGAGGCTGTCGATCAGACGCTCCAGACGCTGTACGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAA‑‑‑‑‑CCCGA‑‑‑CACTAC‑CACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCGCGCAAGACCTGGGCCTCGCGGCAGATCTGCCGGCGCCGTTCGT  >  NC_002947/5959853‑5960143

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`