Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 6,027,222 A→T 5.2% F212Y (TTC→TAC)  PP_5277 ← MFS transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,027,2220AT 5.2% 230.8 / 2.6 77F212Y (TTC→TAC) PP_5277MFS transporter
Reads supporting (aligned to +/- strand):  ref base A (39/34);  new base T (2/2);  total (41/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.82e-01

ACATATGGTCGGCCAGCGCGCCGATGGGCAATTGCAGCACCGCATCACCCACCACCACGGTACTCACCATGAACAAGGCGATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGCG‑AACAGGTCGCCAAAGGTGACACTGCAGGCTTCCGTGCTCGGCGCTCCACCACGCCCCAGCAGAACCAGCGGCGCGGCCAGCAGCAGGCCGGT  >  NC_002947/6027079‑6027370
                                                                                                                                               |                                                                                                                                                     
aCATATGGTCGGCCAGCGCGCCGATGGGCAATTGCAGCACCGCATCACCCACCACCACGGTACTCACCATGAACAAGGCGATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGTTCGAg                                                                                                                                                  <  6:183010/149‑1 (MQ=255)
 cATATGGTCGGCCAGCGCGCCGATGGGCAATTGCAGCACCGCATCACCCACCACCACGGTACTCACCATGAACAAGGCGATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAACGAgg                                                                                                                                                 <  4:207789/149‑1 (MQ=255)
 cATATGGTCGGCCAGCGCGCCGATGGGCAATTGCAGCACCGCATCACCCACCACCACGGTACTCACCATGAACAAGGCGATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAACGAgg                                                                                                                                                 <  6:417826/149‑1 (MQ=255)
  atatGGTCGGCCAGCGCGCCGATGGGCAATTGCAGCACCGCATCACCCACCACCACGGTACTCACCATGAACAAGGCGATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGc                                                                                                                                                >  4:260706/1‑149 (MQ=255)
    atGGTCGGCCAGCGCGCCGATGGGCAATTGCAGCACCGCATCACCCACCACCACGGTACTCACCATGAACAAGGCGATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCg                                                                                                                                               >  8:383915/1‑148 (MQ=255)
      ggTCGGCCAGCGCGCCGATGGGCAATTGCAGCACCGCATCACCCACCACCACGGTACTCACCATGAACAAGGCGATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCGaa                                                                                                                                             >  3:140846/1‑148 (MQ=255)
      ggTCGGCCAGCGCGCCGATGGGCAATTGCAGCACCGCATCACCAACCACCACGGTACTCACCATGAACAAGGCGATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATAGCCTCGTTCGAGGCGat                                                                                                                                             >  5:300608/1‑147 (MQ=255)
       gTCGGCCTGCGCTCCGATGGGCAATTGCAGCACCGCATCACCCACCACCACGGTACTCACCATGAACAAGGCGATTTCGGTGCTGAAGCCCTGCTGCATGCAATACACCGGCAGCAAGGTGAGTATCATCGCCTCGTTCGAGGCGatga                                                                                                                                           <  6:300608/149‑4 (MQ=255)
       gTCGGCCAGCGCGCCGATGGGCAATTGCAGCACCGCATCACCCACCACCACGGTACTCACCATGAACAAGGCGATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCGAACa                                                                                                                                           <  3:190684/149‑1 (MQ=255)
            ccAGCGCGCCGATGGGCAATTGCAGCACCGCATCACCCACCACCACGGTACTCACCATGAACAAGGCGATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAACGAgg                                                                                                                                                 >  5:20282/1‑138 (MQ=255)
            ccAGCGCGCCGATGGGCAATTGCAGCACCGCATCACCCACCACCACGGTACTCACCATGAACAAGGCGATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAACGAgg                                                                                                                                                 <  6:20282/138‑1 (MQ=255)
            ccAGCGCGCCGATGGGCAATTGCAGCACCGCATCACCCACCACCACGGTACTCACCATGAACAAGGCGATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCGAACAgg                                                                                                                                         >  4:76617/1‑146 (MQ=255)
            ccAGCGCGCCGATGGGCAATTGCAGCACCGCATCACCCACCACCACGGTACTCACCATGAACAAGGCGATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCGAACAgg                                                                                                                                         <  3:76617/146‑1 (MQ=255)
                 gcgcCGATGGGCAATTGCAGCACCGCATCACCCACCACCACGGTACTCACCATGAACAAGGCGATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGTTCGAGGCGa                                                                                                                                              >  4:162715/1‑136 (MQ=255)
                  cgcCGATGGGCAATTGCAGCACCGCATCACCCACCACCACGGTACTCACCATGAACAAGGCGATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCGAACAGGGCAATGGcc                                                                                                                                <  2:248938/149‑1 (MQ=255)
                  cgcCGATGGGCAATTGCAGCACCGCATCACCCACCACCACGGTACTCACCATGAACAAGGCGATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCGAACAGGGCAATGGcc                                                                                                                                <  5:346794/149‑1 (MQ=255)
                     cGATGGGCAATTGCAGCACCGCATCACCCACCCCCACGGTACTCACCATGAACAAGGCGATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCGAACAGGGCAATGGCCCAg                                                                                                                             >  1:203354/1‑149 (MQ=255)
                      gATGGGCAATTGCAGCACCGCATCACCCACCACCACGGTACTCACCATGAACAAGGCGATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCGAACAGGGCAATGGCCCAgg                                                                                                                            <  2:230794/149‑1 (MQ=255)
                          ggCAATTGCAGCACCGCATCACCCACCACCACGGTACTCACCATGAACAAGGCGATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCGAACAGGGCAATGGCCCAGGCAAt                                                                                                                        <  3:260706/149‑1 (MQ=255)
                                tGCAGCACCGCATCACCCACCACCACGGTACTCACCATGAACAAGGCGATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCGAACAGGGCAATGGCCCAGGCAATTACCgg                                                                                                                  >  1:270957/1‑149 (MQ=255)
                                      aCCGCATCACCCACCACCACGGTACTCACCATGAACAAGGCGATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCg                                                                                                            >  4:85689/1‑149 (MQ=255)
                                       ccGCATCACCCACCACCACGGTACTCACCATGAACAAGGCGATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGt                                                                                                           >  8:96107/1‑149 (MQ=255)
                                         gCATCACCCACCACCACGGTACTCACCATGAACAAGGCGATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCg                                                                                                         <  1:215972/149‑1 (MQ=255)
                                         gCATCACCCACCACCACGGTACTCACCATGAACAAGGCGATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCg                                                                                                         >  2:103372/1‑149 (MQ=255)
                                          cATCACCCACCACCACGGTACTCACCATGAACAAGGCGATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCgg                                                                                                        <  2:134836/149‑1 (MQ=255)
                                           aTCACCCACCACCACGGTACTCACCATGAACAAGGCGATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCgg                                                                                                        >  7:303174/1‑148 (MQ=255)
                                            tCACCCACCACCACGGTACTCACCATGAACAAGGCGATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCa                                                                                                      >  3:370463/1‑149 (MQ=255)
                                               cccaccACCACGGTACTCACCATGAACAAGGCGATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAg                                                                                                     >  5:5289/1‑147 (MQ=255)
                                                ccaccaccaCGGTACTCACCATGAACAAGGCGATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCGAACAGGGCCATTGccctgttcg                                                                                                                         >  6:187778/1‑120 (MQ=255)
                                                ccaccaccaCGGTACTCACCATGAACAAGGCGATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAg                                                                                                  >  8:263425/1‑149 (MQ=255)
                                                    caccacGGTACTCACCATGAACAAGGCGATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCGAACAGGGAAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGCg                                                                                               >  1:268792/1‑148 (MQ=255)
                                                      ccacGGTACTCACCATGAACAAGGCGATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGCG‑AAc                                                                                           <  6:5289/149‑1 (MQ=255)
                                                              cTCACCATGAACAAGGCGATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGCG‑AACAGGTCGcc                                                                                   <  2:268792/149‑1 (MQ=255)
                                                                 aCCATGAACAAGGCGATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGCG‑AAc                                                                                           <  5:118621/138‑1 (MQ=255)
                                                                 aCCATGAACAAGGCGATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGCG‑AAc                                                                                           >  6:118621/1‑138 (MQ=255)
                                                                  ccATGAACAAGGCGATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGCG‑AACAGGTCGCCAAAg                                                                               <  4:140846/149‑1 (MQ=255)
                                                                   cATGAACAAGGCGATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGCG‑AACAGGTCGCCAAAgg                                                                              >  1:81134/1‑149 (MQ=255)
                                                                   cATGAACAAGGCGATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGCG‑AACAGGTCGCCAAAgg                                                                              >  1:95615/1‑149 (MQ=255)
                                                                       aaCAAGGCGATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGCG‑AACAGGTCGCCAAAGGTGa                                                                           <  7:201560/148‑1 (MQ=255)
                                                                       aaCAAGGCGATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGCG‑AACAGGTCGCCAAAGGTGa                                                                           <  1:143702/148‑1 (MQ=255)
                                                                        aCAAGGCGATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGCG‑AACAGGTCGCCAAAGGTGaca                                                                         <  7:383915/149‑1 (MQ=255)
                                                                             gCGATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGCG‑AACAGGTCGCCAAAGGTGACACTGCa                                                                    <  2:81134/149‑1 (MQ=255)
                                                                               gATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGCG‑AACAGGTCGCCAAAGGTGACACTGCAgg                                                                  >  4:144097/1‑149 (MQ=255)
                                                                                    cGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGCG‑AACAGGTCGCCAAAGGTGACACTGCAGGCTTcc                                                             >  1:153247/1‑149 (MQ=255)
                                                                                     ggTCCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGCG‑AACAGGTCGCCAAAGGTGACACTGCAGGCTTCCg                                                            <  7:263425/149‑1 (MQ=255)
                                                                                          tGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGCG‑AACAGGTCGCCAAAGGTGACACTGCAGGCTTCCGTGCTc                                                       >  2:394835/1‑149 (MQ=255)
                                                                                           gAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGCG‑AACAGGTCGCCAAAGGTGACACTGCAGGCTTCCGTGCTCg                                                      >  4:332405/1‑149 (MQ=255)
                                                                                             aGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGCG‑AACAGGTCGCCAAAGGTGACACTGCAGGCTTCCGTGCTCGgc                                                    >  3:115177/1‑149 (MQ=255)
                                                                                               cccTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGCG‑AACAGGTCGCCAAAGGTGACACTGCAGGCTTCCGTGCTCGgcgc                                                  <  2:139407/149‑1 (MQ=255)
                                                                                                 ctgctgCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGCG‑AACAGGTCGCCAAAGGTGACACTGCAGGCTTCCGTGCTCGGCGCTc                                                <  2:203354/149‑1 (MQ=255)
                                                                                                   gctgcAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGCG‑AACAGGTCGCCAAAGGTGACACTGCAGGCTTCCGTGCTCGGCGCTcca                                              >  7:228697/1‑149 (MQ=255)
                                                                                                    ctgcAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGCG‑AACAGGTCGCCAAAGGTGACACTGCAGGCTTCCGTGCTCGGCGCTccac                                             <  7:96107/149‑1 (MQ=255)
                                                                                                      gcAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGCG‑AACAGGTCGCCAAAGGTGACACTGCAGGCTTCCGTGCTCGGCGCTccacca                                           >  8:350585/1‑149 (MQ=255)
                                                                                                       cAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGCG‑AACAGGTCGCCAAAGGTGACACTGCAg                                                                   >  7:32675/1‑124 (MQ=255)
                                                                                                       cAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGCG‑AACAGGTCGCCAAAGGTGACACTGCAg                                                                   <  8:32675/124‑1 (MQ=255)
                                                                                                       cAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGCG‑AACAGGTCGCCAAAGGTGACACTGCAGGCTTCCGTGCTCGGCGCTCcaccac                                          >  2:340427/1‑149 (MQ=255)
                                                                                                        agtcAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGCGCAACAGGTCGCCAAAGGTGACACTGCAGGCTTCCGTGCTCGGCGCTCcaccac                                          <  1:100631/146‑1 (MQ=255)
                                                                                                            aaTACACCGGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGCG‑AACAGGTCGCCAAAGGTGACACTGCAGGCTTCCGTGCTCGGCGCTCCACCACGccc                                      >  3:390431/1‑148 (MQ=255)
                                                                                                             aTACACCGGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGCG‑AACAGGTCGCCAAAGGTGACACTGCAGGCTTCCGTGCTCGGCGCTCCACCACGCCCca                                    <  2:95615/149‑1 (MQ=255)
                                                                                                                 acCGGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGCG‑AACAGGTCGCCAAAGGTGa                                                                           <  8:290204/106‑1 (MQ=255)
                                                                                                                 acCGGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGCG‑AACAGGTCGCCAAAGGTGa                                                                           >  7:290204/1‑106 (MQ=255)
                                                                                                                 acCGGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGCG‑AACAGGTCGCCAAAGGTGACACTGCAGGCTTCCGTGCTCGGCGCTCCCCCACGCCCcag                                   >  7:18917/1‑146 (MQ=255)
                                                                                                                 acCGGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGCG‑AACAGGTCGCCAAAGGTGACACTGCAGGCTTCCGTGCTCGGCGCTCCACCACGCCCcag                                   >  4:323962/1‑146 (MQ=255)
                                                                                                                 acCGGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGCG‑AACAGGTCGCCAAAGGTGACACTGCAGGCTTCCGTGCTCGGCGCTCCACCACGCCCcag                                   >  4:132706/1‑146 (MQ=255)
                                                                                                                    ggCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGCG‑AACAGGTCGCCAAAGGTGACACTGCAGGCTTCCGTGCTCGGCGCTCCACCACGCCCCAGCAGAAc                             >  1:199108/1‑149 (MQ=255)
                                                                                                                      cagcaAGGTGAGGATCATCGCCTCGAACGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGCG‑AACAGGTCGCCAAAGGTGACACTGCATGCTTCCGTGCTCGGCGCTCCACCACGCCCCAGCAGAACCa                           <  4:115177/149‑1 (MQ=255)
                                                                                                                      cagcaAGGTGAGGATCATCGCCTCGAACGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGCG‑AACAGGTCGCCAAAGGTGACACTGCAGGCTTCCGTGCTCGGCGCTCCACCACGc                                        >  7:161470/1‑136 (MQ=255)
                                                                                                                      cagcaAGGTGAGGATCATCGCCTCGAACGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGCG‑AACAGGTCGCCAAAGGTGACACTGCAGGCTTCCGTGCTCGGCGCTCCACCACGc                                        <  8:161470/136‑1 (MQ=255)
                                                                                                                         caAGGTGAGGATCATCGCCTCGAACGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGCG‑AACAGGTCGCCAAAGGTGACACTGCAGGCTTCCGTGCTCGGCGCTCCACCACGCCCCAGCAGAACCAgcg                        <  2:270957/149‑1 (MQ=255)
                                                                                                                           aGGTGAGGATCATCGCCTCGAACGAGGCGAACAGGCCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGAG‑AACAGGTCGCCAAATGTGACACTGCAGGCTACCGTGCTCGGCGCTCCACCACGCCCCAGCAGAACCAgaggc                      >  2:78468/1‑149 (MQ=255)
                                                                                                                              tGAGGATCCTCGCCTCGAACGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGCG‑AACAGGTCGCCAAAGGTGACACTGCAGGCTTCCGTGCTCGGCGCTCCACCACGCCCCAGCAGAACCAgcggcgcg                   <  8:160105/149‑1 (MQ=255)
                                                                                                                                aGGATCATCGCCTCGAACGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGCG‑AACAGGTCGCCAAAGGTGACACTGCAGGCTTCCGTGCTCGGCGCTCCACCACGCCCCAGCAGAACCAgcggcgcggc                 <  4:390431/149‑1 (MQ=255)
                                                                                                                                    tgttcGCCTCGAACGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGCG‑AACAGGTCGCCAAAGGTGACACTGCAGGCTTCCGTGCTCGGCGCTCCACCACGCCCCAGCAGAACCAGCGGCGCGGCcag              >  2:327769/4‑148 (MQ=255)
                                                                                                                                      atcGCCTCGAACGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGCG‑AACAGGTCGCCAAAGGTGACACTGCAGGCTTCCGTGCTCGGCGCTCCACCACGCCCCAGCAGAACCAGCGGCGCGGCcagca            <  3:85689/148‑1 (MQ=255)
                                                                                                                                      atcGCCTCGAACGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGCG‑AACAGGTCGCCAAAGGTGACACTGCAGGCTTCCGTGCTCGGCGCTCCACCACGCCCCAGCAGAACCAGCGGCGCGGCcagca            <  7:350585/148‑1 (MQ=255)
                                                                                                                                       tcGCCTCGAACGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGCG‑AACAGGTCGCCAAAGGTGACACTGCAGGCTTCCGTGCTCGGCGCTCCACAACGCCCCAGCAGAACCAGCGGCGCGGCcagcagc          >  7:320281/1‑149 (MQ=255)
                                                                                                                                              gAACGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGCG‑AACAGGTCGCCAAAGGTGACACTGCAGGCTTCCGTGCTCGGCGCTCCACCACGCCCCAGCAGAACCAGCGGCGCGGCCAGCAGCAGGCCgg   >  8:223005/1‑149 (MQ=255)
                                                                                                                                               aaCGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGCG‑AACAGGTCGCCAAAGGTGACACTGCAGGCTTCCGTGCTCGGCGCTCCACCACGCCCCAGCAGAACCAGCGGCGCGGCCAGCAGCAGGCCGGt  >  7:113335/1‑149 (MQ=255)
                                                                                                                                               aaCGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGCG‑AACAGGTCGCCAAAGGTGACACTGCAGGCTTCCGTGCTCGGCGCTCCACCACGCCCCAGCAGAACCAGCGGCGCGGCCAGCAGCAGGCCGGt  <  7:223005/149‑1 (MQ=255)
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ACATATGGTCGGCCAGCGCGCCGATGGGCAATTGCAGCACCGCATCACCCACCACCACGGTACTCACCATGAACAAGGCGATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGCG‑AACAGGTCGCCAAAGGTGACACTGCAGGCTTCCGTGCTCGGCGCTCCACCACGCCCCAGCAGAACCAGCGGCGCGGCCAGCAGCAGGCCGGT  >  NC_002947/6027079‑6027370

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑