Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 6,061,252 T→C 8.0% D39G (GAC→GGC)  hupA ← DNA‑binding protein HU‑alpha

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,061,2520TC 8.0% 247.2 / 9.7 87D39G (GAC→GGC) hupADNA‑binding protein HU‑alpha
Reads supporting (aligned to +/- strand):  ref base T (42/38);  new base C (3/4);  total (45/42)
Fisher's exact test for biased strand distribution p-value = 7.07e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.51e-01

ACGCTGTCACGCAGGTTCTTGCCAGGCTTGAAGGCCACGGTATTGCTGGCCTTGATCTTGACCGGTTCCCCAGTTTGCGGGTTCTTGCCGGTACGCGCACCGCGATGACGTTTTTCG‑AAGGTGCCGAAACCGACCAGGGTCACGGTGTCCTTGTCCAGGGCACCGGTGATGCTGTCGAGAATCGCGTTCAAAACCTGATTGGCCTTTTCCTTGGTCAGATCAGCCTTATCGGCGATGACGGCGGCGAGTTCTGGTTTGCGCATAGTGAAGCCTCTTTGACGGAATTCTT  >  NC_002947/6061105‑6061393
                                                                                                                                                    |                                                                                                                                             
actcTGTCACGCATGTTCTTGCCAGGCTTGAAGGCCACGGTATTGCTGGCCTTGATCTTGACCGGTTCCCCATTTTGCGGGTTCTTGCCGGTACTCGCACCGCGATGACGTTTTTCG‑AAGGTGCCGAAACCGACCAGGGTCACGgtgt                                                                                                                                               <  5:327515/145‑1 (MQ=255)
 cGCTGTCACGCAGGTTCTTGCCAGGCTTGAAGGCCACGGTATTGCTGGCCTTGATCTTGACCGGTTCCCCAGTTTGCGGGTTCTTGCCGGTACGCGCACCGCGATGACGTTTTTCG‑AAGGTGCCGAAACCGACCAGGGTCACGGTGTcc                                                                                                                                             <  5:64841/149‑1 (MQ=255)
  gCTGTCACGCAGGTTCTTGCCAGGCTTGAAGGCCACGGTATTGCTGGCCTTGATCTTGACCGGTTCCCCAGTTTGCGGGTTCTTGCCGGTACGCGCACCGCGATGACGTTTTTCG‑AAGGTGCCGAAACCGACCAGGGTCACGGTGTCCt                                                                                                                                            >  2:132784/1‑149 (MQ=255)
   cTGTCACGCAGGTTCTTGCCAGGCTTGAAGGCCACTGTATTGCTGGCCTTGATCTTGACCGGTTCCCCAGTTTGCGGGTTCTTGCCGGTACGCGCACCGCGATGACGTTTTTCG‑AAGGTGCCGAAACCGACCAGGGTCACGGTGTCCtt                                                                                                                                           <  2:100220/149‑1 (MQ=255)
   cTGTCACGCAGGTTCTTGCCAGGCTTGAAGGCCACGGTATTGCTGGCCTTGATCTTGACCGGTTCCCCAGTTTGCGGGTTCTTGCCGGTACGCGCACCGCGATGACGTTTTTCG‑AAGGTGCCGAAACCGACCAGGGTCACGGTGTCCtt                                                                                                                                           <  2:238938/149‑1 (MQ=255)
     gTCACGCAGGTTCTTGCCAGGCTTGAAGGCCACGGTATTGCTGGCCTTGATCTTGACCGGTTCCCCAGTTTGCGGGTTCTTGCCGGTACGCGCACCGCGATGACGTTTTTCG‑AAGGTGCCGAAACCGACCAGGGTCACGGTGTc                                                                                                                                              <  5:417478/144‑1 (MQ=255)
     gTCACGCAGGTTCTTGCCAGGCTTGAAGGCCACGGTATTGCTGGCCTTGATCTTGACCGGTTCCCCAGTTTGCGGGTTCTTGCCGGTACGCGCACCGCGATGACGTTTTTCG‑AAGGTGCCGAAACCGACCAGGGTCACGGTGTc                                                                                                                                              >  6:417478/1‑144 (MQ=255)
      tCACGCAGGTTCTTGCCAGGCTTGAAGGCCACGGTATTGCTGGCCTTGATCTTGACCGGTTCCCCAGTTTGCGGGTTCTTGCCGGTACGCGCACCGCGATGACGTTTTTCG‑AAGGTGCCGAAACCGACCAGGGTCACGGTGTCCTTGTc                                                                                                                                        >  1:387746/1‑149 (MQ=255)
              gTTCTTGCCAGGCTTGAAGGCCACGGTATTGCTGGCCTTGATCTTGACCGGTTCCCCAGTTTGCGGGTTCTTGCCGGTACGCGCACCGCGATGACGTTTTTCG‑AAGGTGCCGAAACCGACCAGGGTCACGGTGCCCTGGACAAGGacac                                                                                                                                <  3:147162/149‑1 (MQ=255)
               ttCTTGCCAGGCTTGAAGGCCACGGTATTGCTGGCCTTGATCTTGACCGGTTCCCCAGTTTGCGGGTTCTTGCCGGTACGCGCACCGCGATGACGTTTTTCG‑AAGGTGCCGAAACCGACCAGGGTCACGGTGCCCTGGACAAGGACAcc                                                                                                                               <  1:357503/149‑1 (MQ=255)
               ctCTTGCCAGGCTTGAAGGCCACGGTATTGCTGGCCTTGATCTTGACCGGTTCCCCAGTTTGCGGGTTCTTGCCGGTACGCGCACCGCGATGACGTTTTTCG‑AAGGTGCCGAAACCGACCAGGGTCACGGTGCCCTGGACAAGGACAcc                                                                                                                               <  8:141302/148‑1 (MQ=255)
                    gCCAGGCTTGAAGGCCACGGTATTGCTGGCCTTGATCTTGACCGGTTCCCCAGTTTGCGGGTTCTTGCCGGTACGCGCACCGCGATGACGTTTTTCG‑AAGGTGCCGAAACCGACCAGGGTCACGGTGTCCTTGTCCAGGGCACCGGTGa                                                                                                                          >  1:119218/1‑149 (MQ=255)
                     ccAGGCTTGAAGGCCACGGTATTGCTGGCCTTGATCTTGACCGGTTCCCCAGTTTGCGGGTTCTTGCCGGTACGCGCACCGCGATGACGTTTTTCG‑AAGGTGCCGAAACCGACCAGGGTCACGGTGTCCTTGTCCa                                                                                                                                      <  7:218489/136‑1 (MQ=255)
                     ccAGGCTTGAAGGCCACGGTATTGCTGGCCTTGATCTTGACCGGTTCCCCAGTTTGCGGGTTCTTGCCGGTACGCGCACCGCGATGACGTTTTTCG‑AAGGTGCCGAAACCGACCAGGGTCACGGTGTCCTTGTCCa                                                                                                                                      >  8:218489/1‑136 (MQ=255)
                     ccAGGCTTGAAGGCCACGGTATTGCTGGCCTTGATCTTGACCGGTTCCCCAGTTTGCGGGTTCTTGCCGGTACGCGCACCGCGATGACGTTTTTCG‑AAGGTGCCGAAACCGACCAGGGTCACGGTGTCCTTGTCCAGGGCACCGGTGAt                                                                                                                         >  8:109742/1‑149 (MQ=255)
                      cAGGCTTGAAGGCCACGGTATTGCTGGCCTTGATCTTGACCGGTTCCCCAGTTTGCGGGTTCTTGCCGGTACGCGCACCGCGATGACGTTTTTCG‑AAGGTGCCGAAACCGACCAGGGTCACGGTGTCCTTGTCCAGGGCACCGGTGATg                                                                                                                        <  2:387746/149‑1 (MQ=255)
                      cAGGCTTGAAGGCCACGGTATTGCTGGCCTTGATCTTGACCGGTTCCCCAGTTTGCGGGTTCTTGCCGGTACGCGCACCGCGATGACGTTTTTCG‑AAGGTGCCGAAACCGACCAGGGTCACGGTGTCCTTGTCCAGGGCACCGGTGATg                                                                                                                        >  7:74787/1‑149 (MQ=255)
                           ttGAAGGCCACGGTATTGCTGGCCTTGATCTTGACCGGTTCCCCAGTTTGCGGGTTCTTGCCGGTACGCGCACCGCGATGACGTTTTTCG‑AAGGTGCCGAAACCGACCAGGGTCACGGTGTCCTTGTCCAGGGCACCGGTGATGCTGTc                                                                                                                   <  2:294878/149‑1 (MQ=255)
                           ttGAAGGCCACGGTATTGCTGGCCTTGATCTTGACCGGTTCCCCAGTTTGCGGGTTCTTGCCGGTACGCGCACCGCGATGACGTTTTTCG‑AAGGTGCCGAAACCGACCAGGGTCACGGTGTCCTTGTCCAGGGCACCGGTGATGCTGTc                                                                                                                   >  1:38625/1‑149 (MQ=255)
                             gAAGGCCACGGTATTGCTGGCCTTGATCTTGACCGGTTCCCCAGTTTGCGGGTTCTTGCCGGTACGCGCACCGCGATGACGTTTTTCG‑AAGGTGCCGAAACCGACCAGGGTCACGGTGTCCTTGTCCAGGGCACCGGTGATGCTGTc                                                                                                                   <  4:232042/147‑1 (MQ=255)
                              aaGGCCACGGGATTGCTGGCCTTGATCTTGACCGGTTCCCCAGTTTGCGGGTTCTTGCCGGTACGCGCACCGCGATGACGTTTTTCG‑AAGGTGCCGAAACCGACCAGGGTCACGGTGTCCTTGTCCAGGGCACCGGTGATGCTGTCga                                                                                                                 <  8:293717/148‑1 (MQ=255)
                               aGGCCACGGTATTGCTGGCCTTGATCTTGACCGGTTCCCCAGTTTGCGGGTTCTTGCCGGTACGCGCACCGCGATGACGTTTTTCG‑AAGGTGCCGAAACCGACCAGGGTCACGGTGTCCTTGTCCAGGGCACCGGTGATGCTGTCgaga                                                                                                               <  2:119218/149‑1 (MQ=255)
                                ggCCACGGTATTGCTGGCCTTGATCTTGACCGGTTCCCCAGTTTGCGGGTTCTTGCCGGTACGCGCACCGCGATGACGTTTTTCG‑AAGGTGCCGAAACCGACCAGGGTCACGGTGTCCTTGTCCAGGGCACCGGTGATGCTGTCGAGaa                                                                                                              <  7:139793/149‑1 (MQ=255)
                                 gCCACGGTATTGCTGGCCTTGATCTTGACCGGTTCCCCAGTTTGCGGGTTCTTGCCGGTACGCGCACCGCGATGACGTTTTTCGTGAGGTGCCGAAACCGACCAGGGTCACGGTGTCCTTGTCCAGGGCACCGGTGATGCTGTCGAGaa                                                                                                              <  1:325129/149‑1 (MQ=255)
                                   cACGGTATTGCTGGCCTTGATCTTGACCGGTTCCCCAGTTTGCGGGTTCTTGCCGGTACGCGCACCGCGATGACGTTTTTCG‑AAGGTGCCGAAACCGACCAGGGTCACGGTGTCCTTGTCCAGGGCACCGGTGATGCTGTCGAGAATcg                                                                                                           >  1:127547/1‑149 (MQ=255)
                                        tATTGCTGGCCTTGATCTTGACCGGTTCCCCAGTTTGCGGGTTCTTGCCGGTACGCGCACCGCGATGACGTTTTTCG‑AAGGTGCCGAAACCGACCAGGGTCACGGTGTCCTTGTCCAGGGCACCGGTGATGCTGTCGAGAATCGCGTTc                                                                                                      <  3:65194/149‑1 (MQ=255)
                                            gCTGGCCTTGATCTTGACCGGTTCCCCAGTTTGCGGGTTCTTGCCGGTACGCGCACCGCGATGACGTTTTTCG‑AAGGTGCCGAAACCGACCAGGGTCACGGTGTCCTTGTCCAGGGCACCGGTGATGCTGTCGAGAATCGCGTTCaaaa                                                                                                  >  2:204869/1‑149 (MQ=255)
                                            gCTGGCCTTGATCTTGACCGGTTCCCCAGTTTGCGGGTTCTTGCCGGTACGCGCACCGCGATGACGTTTTTCG‑AAGGTGCCGAAACCGACCAGGGTCACGGTGTCCTTGTCCAGGGCACCGGTGATGCTGTCGAGAATCGCGTTCaaaa                                                                                                  >  8:120421/1‑149 (MQ=255)
                                            gCTGGCCTTGATCTTGACCGGTTCCCCAGTTTGCGGGTTCTTGCCGGTACGCGCACCGCGATGACGTTTTTCG‑AAGGTGCCGAAACCGACCAGGGTCACGGTGTCCTTGTCCAGGGCACCGGTGATGCTGTCGAGAATCGCGTTCaaaa                                                                                                  >  6:3638/1‑149 (MQ=255)
                                             cTGGCCTTGATCTTGACCGGTTCCCCAGTTTGCGGGTTCTTGCCGGTACGCGCACCGCGATGACGTTTTTCG‑AAGGTGCCGAAACCGACCAGGGTCACGGTGTCCTTGTCCAGGGCACCGGTGATGCTGTCGAGAATCGCGTTCaaaa                                                                                                  <  7:109742/148‑1 (MQ=255)
                                                gCCTTGATCTTGACCGGTTCCCCAGTTTGCGGGTTCTTGCCGGTACGCGCACCGCGATGACGTTTTTCG‑AAGGTGCCGAAACCGACCAGGGTCACGGTGTCCTTGTCCAGGGCACCGGTGATGCTGTCGAGAATCGCGTTCAAAAcc                                                                                                <  4:154742/147‑1 (MQ=255)
                                                gCCTTGATCTTGACCGGTTCCCCAGTTTGCGGGTTCTTGCCGGTACGCGCACCGCGATGACGTTTTTCG‑AAGGTGCCGAAACCGACCAGGGTCACGGTGTCCTTGTCCAGGGCACCGGTGATGCTGTCGAGAATCGCGTTCAAAACCt                                                                                               >  4:33456/1‑148 (MQ=255)
                                                gCCTTGATCTTGACCGGTTCCCCAGTTTGCGGGTTCTTGCCGGTACGCGCACCGCGATGACGTTTTTCG‑AAGGTGCCGAAACCGACCAGGGTCACGGTGTCCTTGTCCAGGGCACCGGTGATGCTGTCGAGAATCGCGTTCAAAACCt                                                                                               >  6:116333/1‑148 (MQ=255)
                                                   ttGATCTTGACCGGTTCCCCAGTTTGCGGGTTCTTGCCGGTACGCGCACCGCGATGACGTTTTTCG‑AAGGTGCCGAAACCGACCAGGGTCACGGTGTCCTTGTCCAGGGCACCGGTGATGCTGTCGAGAATCGCGTTCAAAAcc                                                                                                <  7:344732/144‑1 (MQ=255)
                                                   ttGATCTTGACCGGTTCCCCAGTTTGCGGGTTCTTGCCGGTACGCGCACCGCGATGACGTTTTTCG‑AAGGTGCCGAAACCGACCAGGGTCACGGTGTCCTTGTCCAGGGCACCGGTGATGCTGTCGAGAATCGCGTTCAAAAcc                                                                                                >  8:344732/1‑144 (MQ=255)
                                                    tGATCTTGACCGGTTCCCCAGTTTGCGGGTTCTTGCCGGTACGCGCACCGCGATGACGTTTTTCG‑AAGGTGCCGAAACCGACCAGGGTCACGGTGTCCTTGTCCAGGGCACCGGTGATGCTGTCGAGAATCGCGTTCAAAACCt                                                                                               <  7:165575/144‑1 (MQ=255)
                                                    tGATCTTGACCGGTTCCCCAGTTTGCGGGTTCTTGCCGGTACGCGCACCGCGATGACGTTTTTCG‑AAGGTGCCGAAACCGACCAGGGTCACGGTGTCCTTGTCCAGGGCACCGGTGATGCTGTCGAGAATCGCGTTCAAAACCt                                                                                               >  8:165575/1‑144 (MQ=255)
                                                     gATCTTGACCGGTTCCCCAGTTTGCGGGTTCTTGCCGGTACGCGCACCGCGATGACGTTTTTCG‑AAGGTGCCGAAACCGACCAGGGTCACGGTGTCCTTGTCCAGGGCACCGGTGATGCTGTCGAGAATCGCGTTCAAAACCTGAtt                                                                                           >  6:271911/1‑147 (MQ=255)
                                                     gATCTTGACCGGTTCCCCAGTTTGCGGGTTCTTGCCGGTACGCGCACCGCGATGACGTTTTTCG‑AAGGTGCCGAAACCGACCAGGGTCACGGTGTCCTTGTCCAGGGCACCGGTGATGCTGTCGAGAATCGCGTTCAAAACCTGAtt                                                                                           <  5:271911/147‑1 (MQ=255)
                                                     gATCTTGACCGGTTCCCCAGTTTGCGGGTTCTTGCCGGTACGCGCACCGCGATGACGTTTTTCG‑AAGGTGCCGAAACCGACCAGGGTCACGGTGTCCTTGTCCAGGGCACCGGTGATGCTGTCGAGAATCGCGTTCAAAACCTGATTgg                                                                                         >  5:339838/1‑149 (MQ=255)
                                                      aTCTTGACCGGTTCCCCAGTTTGCGGGTTCTTGCCGGTACGCGCACCGCGATGACGTTTTTCG‑AAGGTGCCGAAACCGACCAGGGTCACGGTGTCCTTGTCCAGGGCACCGGTGATGCTGTCGAGAATCGCGTTCAAAACCTGATTGGc                                                                                        >  3:53045/1‑149 (MQ=255)
                                                         ttGACCGGTTCCCCAGTTTGCGGGTTCTTGCCGGTACGCGCACCGCGATGACGTTTTTCG‑AAGGTGCCGAAACCGACCAGGGTCACGGTGTCCTTGTCCAGGGCACCGGTGATGCTGTCGAGAATCGCGTTCAAAACCTGATTGGCCtt                                                                                     >  2:374056/1‑149 (MQ=255)
                                                           gACCGGTTCCCCAGTTTGCGGGTTCTTGCCGGTACGCGCACCGCGATGACGTTTTTCG‑AAGGTGCCGAAACCGACCAGGGTCACGGTGTCCTTGTCCAGGGCACCGGTGATGCTGTCGAGAATCGCGTTCAAAACCTGATTGGCCtttt                                                                                   >  1:54046/1‑149 (MQ=255)
                                                             ccGGTTCCCCAGTTTGCGGGTTCTTGCCGGTACGCGCACCGCGATGACGTTTTTCG‑AAGGTGCCGAAACCGAGCAGGGTCACGGTGTCCTTGTCCAGGGCACCGGTGATGCTGTCGAGAATCGCGTTCAAAACCTGATTGGCCTTTTcc                                                                                 >  1:33949/1‑149 (MQ=255)
                                                              cGGTTCCCCAGTTTGCGGGTTCTTGCCGGTACGCGCACCGCGATGACGTTTTTCG‑AAGGTGCCGAAACCGACCAGGGTCACGGTGTCCTTGTCCAGGGCACCGGTGATGCTGTCGAGAATCGCGTTCAAAACCTGATTGGCCTTTTCCt                                                                                <  5:3638/149‑1 (MQ=255)
                                                                gTTCCCCAGTTTGCGGGTTCTTGCCGGTACGCGCACCGCGATGACGTTTTTCG‑AAGGTGCCGAAACCGACCAGGGTCACGGTGTCCTTGTCCAGGGCACCGGTGATGCTGTCGAGAATCGCGTTCAAAACCTGATTGGCCTTTTCCTTg                                                                              <  1:204869/149‑1 (MQ=255)
                                                                gTTCCCCAGTTTGCGGGTTCTTGCCGGTACGCGCACCGCGATGACGTTTTTCG‑AAGGTGCCGAAACCGACCAGGGTCACGGTGTCCTTGTCCAGGGCACCGGTGATGCTGTCGAGAATCGCGTTCAAAACCTGATTGGCCTTTTCCTTg                                                                              >  3:287550/1‑149 (MQ=255)
                                                                   ccccAGTTTGCGGGTTCTTGCCGGTACGCGCACCGCGATGACGTTTTTCG‑AAGGTGCCGAAACCGACCAGGGTCACGGTGTCCTTGTCCAGGGCACCGGTGATGCTGTCGAGAATCGCGTTCAAAACCTGATTGGCCTTTTCCTTGGt                                                                            <  8:76598/148‑1 (MQ=255)
                                                                   ccccAGTTTGCGGGTTCTTGCCGGTACGCGCACCGCGATGACGTTTTTCG‑AAGGTGCCGAAACCGACCAGGGTCACGGTGTCCTTGTCCAGGGCACCGGTGATGCTGTCGAGAATCGCGTTCAAAACCTGATTGGCCTTTTCCTTGGt                                                                            >  3:58795/1‑148 (MQ=255)
                                                                    cccAGTTTGCGGGTTCTTGCCGGTACGCGCACCGCGATGACGTTTTTCG‑AAGGTGCCGAAACCGACCAGGGTCACGGTGTCCTTGTCCAGGGCACCGGTGATGCTGTCGAGAATCGCGTTCAAAACCTGATTGGCCTTTTCCTTGGTc                                                                           <  4:287550/148‑1 (MQ=255)
                                                                     ccAGTTTGCGGGTTCTTGCCGGTACGCGCACCGCGATGACGTTTTTCG‑AAGGTGCCGAAACCGACCAGGGTCACGGTGTCCTTGTCCAGGGCACCGGTGATGCTGTCGAGAATCGCGTTCAAAACCTGATTGGCCTTTTcc                                                                                 >  6:368538/1‑141 (MQ=255)
                                                                     ccAGTTTGCGGGTTCTTGCCGGTACGCGCACCGCGATGACGTTTTTCG‑AAGGTGCCGAAACCGACCAGGGTCACGGTGTCCTTGTCCAGGGCACCGGTGATGCTGTCGAGAATCGCGTTCAAAACCTGATTGGCCTTTTcc                                                                                 <  5:368538/141‑1 (MQ=255)
                                                                      cAGTTTGCGGGTTCTTGCCGGTACGCGCACCGCGATGACGTTTTTCG‑AAGGTGCCGAAACCGACCAGGGTCACGGTGTCCTTGTCCAGGGCACCGGTGATGCTGTCGAGAATCGCGTTCAAAACCTGATTGGCCTTTTCCTTGGt                                                                            >  3:42248/1‑145 (MQ=255)
                                                                            gCGGGTTCTTGCCGGTACGCGCACCGCGATGACGTTTTTCG‑AAGGTGCCGAAACCGACCAGGGTCACGGTGTCCTTGTCCAGGGCACCGGTGATGCTGTCGAGAATCGCGTTCAAAACCTGATTGGTCTTTTCCTTGGTCAGATCAGc                                                                   <  7:120421/148‑1 (MQ=255)
                                                                             cGGGTTCTTGCCGGTACGCGCACCGCGATGACGTTTTTCG‑AAGGTGCCGAAACCGACCAGGGTCACGGTGTCCTTGTCCAGGGCACCGGTGATGCTGTCGAGAATCGCGTTCAAAACCTGATTGGCCTTTTCCTTg                                                                              >  4:261036/1‑136 (MQ=255)
                                                                             cGGGTTCTTGCCGGTACGCGCACCGCGATGACGTTTTTCG‑AAGGTGCCGAAACCGACCAGGGTCACGGTGTCCTTGTCCAGGGCACCGGTGATGCTGTCGAGAATCGCGTTCAAAACCTGATTGGCCTTTTCCTTGGTCAGATCAGcc                                                                  <  1:132784/148‑1 (MQ=255)
                                                                                   cTTGCCGGTACGCGCACCGCGATGACGTTTTTCG‑AAGGTGCCGAAACCGACCAGGGTCACGGTGTCCTTGTCCAGGGCACCGGTGATGCTGTCGAGAATCGCGTTCAAAACCTGATTGGCCTTTTCCTTGGTCAGATCAGCCTTATCg                                                            <  4:53045/148‑1 (MQ=255)
                                                                                    ttGCCGGTACGCGCACCGCGATGACGTTTTTCG‑AAGGTGCCGAAACCGACCAGGGTCACGGTGTCCTTGTCCAGGGCACCGGTGATGCTGTCGAGAATCGCGTTCAAAACCTGATTGGCCTTTTCCTTGGTCAGATCAGCCTTATCGGc                                                          >  3:204111/1‑149 (MQ=255)
                                                                                      gCCGGTACGCGCACCGCGATGACGTTTTTCG‑AAGGTGCCGAAACCGAGCAGGGTCACGGTGTCCTTGTCCAGGGCACCGGTGATGCTGTCGAGAATCGCGTTCAAAACCTGATTGGCCTTTTCCTTGGTCAGATCAGCCTTATCGGCg                                                         <  2:33949/148‑1 (MQ=255)
                                                                                      gCCGGTACGCGCACCGCGATGACGTTTTTCG‑AAGGTGCCGAAACCGACCAGGGTCACGGTGTCCTTGTCCAGGGCACCGGTGATGCTGTCGAGAATCGCGTTCAAAACCTGATTGGCCTTTTCCTTGGTCAGATCAGCCTTATCGGc                                                          <  8:74787/147‑1 (MQ=255)
                                                                                      gCCGGTACGCGCACCGCGATGACGTTTTTCG‑AAGGTGCCGAAACCGACCAGGGTCACGGTGTCCTTGTCCAGGGCACCGGTGATGCTGTCGAGAATCGCGTTCAAAACCTGATTGGCCTTTTCCTTGGTCAGATCAGCCTTATCGGCg                                                         <  2:127547/148‑1 (MQ=255)
                                                                                       ccGGTACGCGCACCGCGATGACGTTTTTCG‑AAGGTGCCGAAACCGACCAGGGTCACGGTGTCCTTGTCCAGGGCACCGGTGATGCTGTCGAGAATCGCGTTCAAAACCTGATTGGCCTTTTCCTTGGTCAGATCAGCCTTATCGGCGAt                                                       <  6:339838/149‑1 (MQ=255)
                                                                                          gTACGCGCACCGCGATGACGTTTTTCG‑AAGGTGCCGAAACCGACCAGGGTCACGGTGTCCTTGTCCAGGGCACCGGTGATGCTGTCGAGAATCGCGTTCAAAACCTGATTGGCCTTTTCCTTGGTCAGATCAGCCTTATCGGCGATGAc                                                    >  1:147195/1‑149 (MQ=255)
                                                                                               cgcACCGCGATGACGTTTTTCG‑AATGTGCCGAAACCGACCAGGGTCACGGTGTCCTTGTCCAGGGCACCGGTGATGCTGTCGAGAATCGCGTTCAAAACCTGATTGGCCTTTTCCTTGGTCAGATCAGCCTTATCGGCGATGAcggcgg                                               <  8:60268/149‑1 (MQ=255)
                                                                                                  aCCGCGATGACGTTTTTCG‑AAGGTGCCGAAACCGACCAGGGTCACGGTGTCCTTGTCCAGGGCACCGGTGATGCTGTCGAGAATCGCGTTCAAAACCTGATTGGCCTTTTCCTTGGTCAGATCAGCCTTATCGGCGATGACGGCGGCGa                                            <  3:33456/149‑1 (MQ=255)
                                                                                                             gTTTTTCG‑AAGGTGCCGAAACCGACCAGGGTCACGGTGTCCTTGTCCAGGGCACCGGTGATGCTGTCGAGAATCGCGTTCAAAACCTGATTGGCCTTTTCCTTGGTCAGATCAGCCTTATCGGCGATGACGGCGGCGa                                            >  5:30366/1‑138 (MQ=255)
                                                                                                             gTTTTTCG‑AAGGTGCCGAAACCGACCAGGGTCACGGTGTCCTTGTCCAGGGCACCGGTGATGCTGTCGAGAATCGCGTTCAAAACCTGATTGGCCTTTTCCTTGGTCAGATCAGCCTTATCGGCGATGACGGCGGCGa                                            <  6:30366/138‑1 (MQ=255)
                                                                                                             gTTTTTCG‑AAGGTGCCGAAACCGACCAGGGTCACGGTGTCCTTGTCCAGGGCACCGGTGATGCTGTCGAGAATCGCGTTCAAAACCTGATTGGCCTTTTCCTTGGTCAGATCAGCCTTATCGGCGATGACGGCGGCGAGTTCTGGTTTg                                 >  5:144770/1‑149 (MQ=255)
                                                                                                               ttttCG‑AAGGTGCCGAAACCGACCAGGGTCACGGTGTCCTTGTCCAGGGCACCGGTGATGCTGTCGAGAATCGCGTTCAAAACCTGATTGGCCTTTTCCTTGGTCAGATCAGCCTTATCGGCGATGACGGCGGCGAGTTCTGGTTTgc                                <  4:204111/148‑1 (MQ=255)
                                                                                                                    g‑AAGGTGCCGAAACCGACCAGGGTCACGGTGTCCTTGTCCAGGGCACCGGTGATGCTGTCGAGAATCGCGTTCAAAACCTGATTGGCCTTTTCCTTGGTCAGATCAGCCTTATCGGCGATGACGGCGGCGAGTTCTGGTTTGCGCa                             >  6:292141/1‑146 (MQ=255)
                                                                                                                    g‑AAGGTGCCGAAACCGACCAGGGTCACGGTGTCCTTGTCCAGGGCACCGGTGATGCTGTCGAGAATCGCGTTCAAAACCTGATTGGCCTTTTCCTTGGTCAGATCAGCCTTATCGGCGATGACGGCGGCGAGTTCTGGTTTGCGCATa                           <  5:292141/148‑1 (MQ=255)
                                                                                                                    g‑AAGGTGCCGAAACCGACCAGGGTCACGGTGTCCTTGTCCAGGGCACCGGTGATGCTGTCGAGAATCGCGTTCAAAACCTGATTGGCCTTTTCCTTGGTCAGATCAGCCTTATCGGCGATGACGGCGGCGAGTTCTGGTTTGCGCATa                           <  4:42248/148‑1 (MQ=255)
                                                                                                                      aaGGTGCCGAAACCGACCAGGGTCACGGTGTCCTTGTCCAGGGCACCGGTGATGCTGTCGAGAATCGCGTTCAAAACCTGATTGGCCTTTTCCTTGGTCAGATCAGCCTTATCGGCGATGACGGCGGCGAGTTCTGGTTTGCGCATAGt                         >  6:115853/1‑149 (MQ=255)
                                                                                                                          tGCCGAAACCGACCAGGGTCACGGTGTCCTTGTCCAGGGCACCGGTGATGCTGTCGAGAATCGCGTTCAAAACCTGATTGGCCTTTTCCTTGGTCAGATCAGCCTTATCGGCGATGACGGCGGCGAGTTCTGGTTTGCGCATAGTGAAg                     <  1:374056/149‑1 (MQ=255)
                                                                                                                           gCCGAAACCGACCAGGGTCACGGTGTCCTTGTCCAGGGCACCGGTGATGCTGTCGAGAATCGCGTTCAAAACCTGATTGGCCTTTTCCTTGGTCAGATCAGCCTTATCGGCGATGACGGCGGCGAGTTCTGGTTTGCGCATAGTGAAGc                    >  4:193617/1‑149 (MQ=255)
                                                                                                                            ccGAAACCGACCAGGGTCACGGTGTCCTTGTCCAGGGCACCGGTGATGCTGTCGAGAATCGCGTTCAAAACCTGATTGGCCTTTTCCTTGGTCAGATCAGCCTTATCGGCGATGACGGCGGCGAGTTCTGGTTTGCGCATAGTGAAGcc                   >  7:382610/1‑149 (MQ=255)
                                                                                                                              gAAACCGACCAGGGTCACGGTGTCCTTGTCCAGGGCACCGGTGATGCTGTCGAGAATCGCGTTCAAAACCTGATTGGCCTTTTCCTTGGTCAGATCAGCCTTATCGGCGATGACGGCGGCGAGTTCTGGTTTGCGCATAGTGAAGCctc                 >  8:383527/1‑149 (MQ=255)
                                                                                                                              gAAACCGACCAGGGTCACGGTGTCCTTGTCCAGGGCACCGGTGATGCTGTCGAGAATCGCGTTCAAAACCTGATTGGCCTTTTCCTTGGTCAGATCAGCCTTATCGGCGATGACGGCGGCGAGTTCTGGTTTGCGCATAGTGAAGCctc                 >  5:318806/1‑149 (MQ=255)
                                                                                                                               aaaCCGACCAGGGTCACGGTGTCCTTGTCCAGGGCACCGGTGATGCTGTCGAGAATCGCGTTCAAAACCTGATTGGCCTTTTCCTTGGTCAGATCAGCCTTATCGGCGATGACGGCGGCGAGTTCTGGTTTGCGCATAGTGAAGCctct                >  2:164900/1‑149 (MQ=255)
                                                                                                                                    gACCAGGGTCACGGTGTCCTTGTCCAGGGCACCGGTGATGCTGTCGAGAATCGCGTTCAAAACCTGATTGGCCTTTTCCTTGGTCAGATCAGCCTTATCGGCGATGACGGCGGCGAGTTCTGGTTTGCGCATAGTGAAGCCTCTTTGAc           >  4:296275/1‑149 (MQ=255)
                                                                                                                                         gggTCACGGTGTCCTTGTCCAGGGCACCGGTGATGCTGTCGAGAATCGCGTTCAAAACCTGATTGGCCTTTTCCTTGGTCAGATCAGCCTTATCGGCGATGACGGCGGCGAGTTCTGGTTTGCGCATAGTGAAGCCTCTTTGACGGAAt      >  2:270745/1‑149 (MQ=255)
                                                                                                                                           tcaccGGTGCCCTGGACAAGGACACCGGTGATGCTGTCGAGAATCGCGTTCAAAACCTGATTGGCCTTTTCCTTGGTCAGATCAGCCTTATCGGCGATGACGGCGGCGAGTTCTGGTTTGCGCATAGTGAAGCCTCTTTGAc           >  7:305720/5‑142 (MQ=255)
                                                                                                                                           tcaccGGTGCCCTGGACAAGGACACCGGTGATGCTGTCGAGAATCGCGTTCAAAACCTGATTGGCCTTTTCCTTGGTCAGATCAGCCTTATCGGCGATGACGGCGGCGAGTTCTGGTTTGCGCATAGTGAAGCCTCTTTGAc           <  8:305720/138‑1 (MQ=255)
                                                                                                                                            tCACGGTGTCCTTGTCCAGGGCACCGGTGATGCTGTCGAGAATCGCGTTCAAAACCTGATTGGCCCTTTCCTTGGTCAGATCAGCCTTATCGGCGATGACGGCGGCGAGTTCTGGTTTGCGCATAGTGAAGCCTCTTTGACGGAATTCt   >  8:78073/1‑149 (MQ=255)
                                                                                                                                            tCACGGTGTCCTTGTCCAGGGCAACGGTGATGCTGTCGAGAATCGCGTTCAAAACCTGATTGGCCTTTTCCTTGGTCAGATCAGCCTTATCGGCGATGACGGCGGCGAGTTCTGGTTTGCGCATAGTGAAGCCTCTTTGACGGAATTCt   >  6:366920/1‑149 (MQ=255)
                                                                                                                                            caccGGTGCCCTGGACAAGGACACCGGTGATGCTGTCGAGAATCGCGTTCAAAACCTGATTGGCCTTTTCCTTGGTCAGATCAGCCTTATCGGCGATGACGGCGGCGAGTTCTGGTTTGCGCATAGTGAAGCCTCTTTGACGGAATTCt   >  3:181644/4‑149 (MQ=255)
                                                                                                                                            caccGGTGCCCTGGACAAGGACACCGGTGATGCTGTCGAGAATCGCGTTCAAAACCTGATTGGCCTTTTCCTTGGTCAGATCAGCCTTATCGGCGATGACGGCGGCGAGTTCTGGTTTGCGCATAGTGAAGCCTCTTTGACGGAATTCt   >  5:397946/4‑149 (MQ=255)
                                                                                                                                              aCGGTGTCCTTGTCCAGGGCACCGGTGATGCTGTCGAGAATCACGTTCAAAACCTGATTGGCCTTTTCCTTGGTCAGATCAGCCTTATCGGCGATGACGGCGGCGAGTTCTGGTTTGCGCATAGTGAAGCCTCTTTGACGGAATTCtt  <  7:314637/148‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                             
ACGCTGTCACGCAGGTTCTTGCCAGGCTTGAAGGCCACGGTATTGCTGGCCTTGATCTTGACCGGTTCCCCAGTTTGCGGGTTCTTGCCGGTACGCGCACCGCGATGACGTTTTTCG‑AAGGTGCCGAAACCGACCAGGGTCACGGTGTCCTTGTCCAGGGCACCGGTGATGCTGTCGAGAATCGCGTTCAAAACCTGATTGGCCTTTTCCTTGGTCAGATCAGCCTTATCGGCGATGACGGCGGCGAGTTCTGGTTTGCGCATAGTGAAGCCTCTTTGACGGAATTCTT  >  NC_002947/6061105‑6061393

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑