Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	6,080,989	T→A	17.6%	intergenic (+36/+26)	PP_5332 → / ← PP_5333	hypothetical protein/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	6,080,989	0	T	A	17.6%	113.9 / 15.2	54	intergenic (+36/+26)	PP_5332/PP_5333	hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (26/17);  new base A (3/6);  total (29/25)
Fisher's exact test for biased strand distribution p-value = 1.61e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GATGGCAGCGAATGCTGTGCATCGAGACAGCGAATGTGTGGGATGACCTGGTGGAGTTGAAGCCTGGGGCGAGTCATTCGCTGGGCGTCCAGATCGGTTGTGAAAATATCTGAGCCCTGCTCTTTTTTTACCGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGGG‑‑CAATTATCAAAGGTCTGCGTCTTCCACCACCCTCACCTTGTCTGCATCCAGCGCATAGGCCGCATCGGCCAGGTCATTGCTGACCTTCTCCACCTTCAGCTTGCCGCTGACCCACAGCGGGGTGTAGAT  >  NC_002947/6080841‑6081137                                                                                                                                                     |                                                                                                                                                       gatggCAGCGAATGCTGTGCATCGAGACAGCGAATGTGTGGGATGACCTGGTGGAGTTGAAGCCTGGGGCGAGTCATTCGCTGGGCGTCCAGATCGGTTGTGAAAATATCTGAGCCCTGCTCTTTTTTTACCGGCCTCTTCGCGGGCtt                                                                                                                                                        <  3:360165/149‑1 (MQ=255)   tggCAGCGAATGCTGTGCATCGAGACAGCGAATGTGTGGGATGACCTGGTGGAGTTGAAGCCTGGGGCGAGTCATTCGCTGGGCGTCCAGATCGGTTGTGAAAATATCTGAGCCCTGCTCTTTTTTTACCGGCCTCTTCGCGGGCTTGc                                                                                                                                                      >  7:336442/1‑149 (MQ=255)      cAGCGAATGCTGTGCATCGAGACAGCGAATGTGTGGGATGACCTGGTGGAGTTGAAGCCTGGGGCGAGTCATTCGCTGGGCGTCCAGATCGGTTGTGAAAATATCTGAGCCCTGCTCTTTTTTTACCGGCCTCTTCGCGGGCTTGCCcg                                                                                                                                                   >  7:233369/1‑149 (MQ=255)      cAGCGAATGCTGTGCATCGAGACAGCGAATGTGTGGGATGACCTGGTGGAGTTGAAGCCTGGGGCGAGTCATTCGCTGGGCGTCCAGATCGGTTGTGAAAATATCTGAGCCCTGCTCTTTTTTTACCGGCCTCTTCGCGGGCTTGCCcg                                                                                                                                                   >  2:365767/1‑149 (MQ=255)      cAGCGAATGCTGTGCATCGAGACAGCGAATGTGTGGGATGACCTGGTGGAGTTGAAGCCTGGGGCGAGTCATTCGCTGGGCGTCCAGATCGGTTGTGAAAATATCTGAGCCCTGCTCTTTTTTTACCGGCCTCTTCGCGGGCTTGCCcg                                                                                                                                                   >  6:16208/1‑149 (MQ=255)          gAATGCTGTGCATCGAGACAGCGAATGTGTGGGATGACCTGGTGGAGTTGAAGCCTGGGGCGAGTCATTCGCTGGGCGTCCAGATCGGTTGTGAAAATATCTGAGCCCTGCTCTTTTTTTACCGGCCTCTTCGCGGGCTTGCCCGCGaa                                                                                                                                               <  1:164576/149‑1 (MQ=255)          gAATGCTGTGCATCGAGACAGCGAATGTGTGGGATGACCTGGTGGAGTTGAAGCCTGGGGCGAGTCATTCGCTGGGCGTCCAGATCGGTTGTGAAAATATCTGAGCCCTGCTCTTTTTTTACCGGCCTCTTCGCGGGCAAGCCCGCGaa                                                                                                                                               <  5:141116/149‑1 (MQ=255)          gAATGCTGTGCATCGAGACAGCGAATGTGTGGGATGACCTGGTGGAGTTGAAGCCTGGGGCGAGTCATTCGCTGGGCGTCCAGATCGGTTGTGAAAATATCTGAGCCCTGCTCTTTTTTTACCGGCCTCTTCGCGGGCAAGCCCGCGaa                                                                                                                                               <  3:53430/149‑1 (MQ=255)          gAATGCTGTGCATCGAGACAGCGAATGTGTGGGATGACCTGGTGGAGTTGAAGCCTGGGGCGAGTCATTCGCTGGGCGTCCAGATCGGTTGTGAAAATATCTGAGCCCTGCTCTTTTTTTACCGGCCTCTTCGCGGGCAAGCCCGCGaa                                                                                                                                               <  5:408818/149‑1 (MQ=255)           aaTGCTGTGCATCGAGACAGCGAATGTGTGGGATGACCTGGTGGAGTTGAAGCCTGGGGCGAGTCATTCGCTGGGCGTCCAGATCGGTTGTGAAAATATCTGAGCCCTGCTCTTTTTTTACCGGCCTCTTCGCGGGCAAGCCCGCGAag                                                                                                                                              <  5:126334/149‑1 (MQ=255)            ctGCTCTGCATCGAGACCCCGAATGTGGGGGACGACCTGCTGGAGTTGAAGCCTGGGGCGAGTCATTCGCTGGGCGTCCAGATCGGTTGTGAAAATATCTGAGCCCTGCTCTTTATTTACCGGCCTCATCGCGGGCTCGCCCGCGAaga                                                                                                                                             <  4:228610/148‑1 (MQ=255)             tGCTGTGCATCGAGACAGCGAATGTGTGGGATGACCTGGTGGAGTTGAAGCCTGGGGCGAGTCATTCGCTGGGCGTCCAGATCGGTTGTGAAAATATCTGAGCCCTGCTCTTTTTTTACCGGCCTCTTCGCGGGCTTGCCCGCGAagag                                                                                                                                            <  8:197699/149‑1 (MQ=255)                  tgCATCGAGACAGCGAATGTGTGGGATGACCTGGTGGAGTTGAAGCCTGGGGCGAGTCATTCGCTGGGCGTCCAGATCGGTTGTGAAAATATCTGAGCCCTGCTCTTTTTTTACCGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCgg                                                                                                                                       >  4:123771/1‑149 (MQ=255)                   gCATCGAGACAGCGAATGTGTGGGATGACCTGGTGGAGTTGAAGCCTGGGGCGAGTCATTCGCTGGGCGTCCAGATCGGTTGTGAAAAAATCTGAGCCCTGCTCTTTTTTTACCGGCCTCTTAGCGGGCTTGCCCGCGAAGCGGCCggg                                                                                                                                      >  2:408128/1‑149 (MQ=255)                           aCAGCGAATGTGTGGGATGACCTGGTGGAGTTGAAGCCTGGGGCGAGTCATTCGCTGGGCGTCCAGATCGGTTGTGAAAATATCTGAGCCCTGCTCTTTTTTTACCGGCCTCTTCGGGGGCTTTCCCGGGAAGAGGGCGGGG‑‑GAATTa                                                                                                                             >  7:136788/1‑148 (MQ=255)                              gggAATGTGTGGGATGACCTGGTGGAGTTGAAGCCTGGGGCGAGTCATTCGCTGGGCGGCCAGATCGGTTGTGAAAATATCTGAGCCCTGCTCTTTTTTTACCGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGGG‑‑CAATTATCaa                                                                                                                         <  3:377121/147‑1 (MQ=255)                                gAATGTGTGGGAGGACCTGGTGGAGTTGAAGCCTGGGGCGAGTCATTCGCTGGGCGTCCAGATCGGTTGTGAAGATATCTGAGCCCTGCTCTTTTTTTACCGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGGG‑‑CAATTATCAAAg                                                                                                                       >  1:364106/1‑149 (MQ=255)                                  ttgggtgGGATGACCTGGTGGAGTTGAAGCCGGGGGCGAGTCATTCGCTGGGCGTCCAGTTCGGTTGTGAAAATATCTGAGCCCTGCTCTTTTTTTACCGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGGG‑‑CAATTATCAAAgg                                                                                                                      <  4:234241/144‑1 (MQ=255)                                   tgtgtgGGATGACCTGGTGGAGTTGAAGCCTGGGGCGAGTCATTCGCTGGGCGTCCAGATCGGTTGTGAAAATATCTGAGCCCTGCTCTTTTTTTACCGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGGG‑‑CAATTATCAAAGGt                                                                                                                     <  7:161164/148‑1 (MQ=255)                                       tgGTATGACCTGGTGGAGTTGAAGCCTGGGGCGAGTCATTCGCTGGGCGTCCAGATCGGTTGTGAAAATATCTGAGCCCTGCTCTTTTTTTACCGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGGG‑‑CAATTATCAAAGGTCTGc                                                                                                                 <  8:233369/148‑1 (MQ=255)                                         ggATGACCTGGTGGAGTTGATGCCTGGGGCGAGTCATTCGCTGGGCGTCCAGATCGGTTGTGAAAATATCTGAGCCCTGCTCTTTTTTTACCGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGGG‑‑CAATTATCAAAGGTCTGCGt                                                                                                               <  5:401915/148‑1 (MQ=255)                                         ggATGACCTGGTGGAGTTGAAGCCTGGGGCGAGTCATTCGCTGGGCGTCCAGATCGGTTGTGAAAATATCTGAGCCCTGCTCTTTTTTTACCGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGGG‑‑CAATTATCAAAGGTCTGCGTc                                                                                                              >  4:170394/1‑149 (MQ=255)                                            tGACCTGGTGGAGTTGAAGCCTGGGGCGAGTCATTCGCTGGGCGTCCAGATCGGTTGTGAAAATATCTGAGCCCTGCTCTTTTTTTACCGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGGG‑‑CAATTATCAAAGGTCTGCGTCTTc                                                                                                           <  3:123771/149‑1 (MQ=255)                                              tccTGGTGGAGTTGAAGCCTGGGGGTAGTCATTCGCTGGGCGTCCAGATCGTTTGTGAAAATATCTGAGCCCTGCTCTTTTTTTACCGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGGG‑‑CAATTATCAAAGGTCTGCGTCTTcca                                                                                                         <  1:140995/148‑1 (MQ=255)                                               ccTGGTGGAGTTGAAGCCTGGGGCGAGTCATTCGCTGGGCGTCCAGATCGGTTGTGAAAATATCTGAGCCCTGCTCTTTTTTTACCGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGGG‑‑CAATTATCAAAGGTCTGCGTCTTccac                                                                                                        <  6:169494/149‑1 (MQ=255)                                                    tggAGTTGAAGCCTGGGGCGAGTCATTCGCTGGGCGTCCAGATCGGTTGTGAAAATATCTGAGCCCTGCTCTTTTTTTACCGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGGG‑‑CAATTATCAAAg                                                                                                                       >  2:258047/1‑129 (MQ=255)                                                    tggAGTTGAAGCCTGGGGCGAGTCATTCGCTGGGCGTCCAGATCGGTTGTGAAAATATCTGAGCCCTGCTCTTTTTTTACCGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGGG‑‑CAATTATCAAAg                                                                                                                       <  1:258047/129‑1 (MQ=255)                                                         ttGAAGCCTGGGGCGAGTCATTCGCTGGGCGTCCAGATCGGTTGTGAAAATATCTGAGCCCTGCTCTTTTTTTACCGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGGG‑‑CAATTATGAAAGGTCTGCGTCTTCCACCACCCTCAcc                                                                                              >  1:133163/1‑149 (MQ=255)                                                                      cGAGTCATTCGCTGGGCGTCCAGATCGGTTGTGAAAATATCTGAGCCCTGCTCTTTTTTTACCGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGGG‑‑CAATTATCAAAGGTCTGCGTCTTCCACCACCCTCACCTTGTCTGCATc                                                                                   >  5:386514/1‑147 (MQ=255)                                                                       gAGTCATTCGCTGGGCGTCCAGATCGGTTGTGAAAATATCTGAGCCCTGCTCTTTTTTTACCGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGGG‑‑CAATTATCAAAGGTCTGCGTCTTCCACCACCCTCACCTTGTCTGCATCCAg                                                                                >  4:228771/1‑149 (MQ=255)                                                                                gCTGGGCGTCCAGATCGGTTGTGAAAATATCTGAGCCCTGCTCTTTTTTTACCGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGGG‑‑CAATTATCAAAGGTCTGCGTCTTCCACCACCCTCACCTTGTCTGCATCCAGCGCATAGGc                                                                       >  3:70421/1‑149 (MQ=255)                                                                                  tGGGCGTCCAGATCGGTTGTGAAAATATCTGAGCCCTGCTCTTTTTTTACCGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGGG‑‑GAATTATCAAAGGTCTGCGTCTTCCACCACCCGCACCTTGTCTGCATACAGCGCATAgatag                                                                     >  1:186651/1‑145 (MQ=255)                                                                                        tCCAGATCGGTTGTGAAAATATCTGAGCCCTGCTCTTTTTTTACCGGCCTCTTAGCGGGCTTGCCCGCGAAGAGGCCGGGG‑‑CAATTATCAAAGGTCTGCGTCGTCCACCACCCTCACCTTGTCTGCATCCAGCGCATAGGCCGCATCgg                                                               >  4:344417/1‑149 (MQ=255)                                                                                                       aaaaTATCTGAGCCCTGCTCTTTTTTTACCGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGGGTAGAATTATCAAAGGTCTGCGTCTTCCACCACCCTCACCTTGTCTGCATCCAGCGCATAGGCCGCATCGGCCAGGTCATTGCt                                                  >  1:324606/1‑149 (MQ=255)                                                                                                                 aGCCCTGCTCTTTTTTTACCGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGGG‑‑CAATTATCAAAGGTCTGCGTCTTCCACCACCCTCACCTTGTCTGCATCCAGCGCATAGGCCGCATCGGCCAGGTCATTGCTGACCTTCTCCAc                                      <  6:386514/149‑1 (MQ=255)                                                                                                                  gCCCTGCTCTTTTTTTACCGGCCTCTTCGCGGGCTTGCCCGCGAAGCGGCCGGGG‑‑CAATTATCAAAGGTCTGAGTCTTCCACCACCCTCACCTTgg                                                                                          >  2:117797/1‑95 (MQ=255)                                                                                                                   cccGGCCCTTTTTTTACCGCCCGCTGCCGGGGATGGCCCGGTATGAGGCCGGGG‑‑CAATTATCAAAGGTCTGCGTCTTCCACCACCCTCACCTTGTCTGCATCCAGCGCATAGGCCGCATCGGCCAGGTCATTGCTGACCTTCTCCACCt                                    <  1:408128/149‑1 (MQ=255)                                                                                                                     cTGCTCTTTTTTTACCGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGGG‑‑CAATTATCAAAGGTCTGCGTCTTCCACCACCCTCACCTTGTCTGCATCCAGCGCATAGGCCGCATCGGCCAGGTCATTGCTGACCTTCTCCACCtt                                   >  1:152125/1‑148 (MQ=255)                                                                                                                      tGCTCTTTTTTTACCGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGGG‑‑CAATTATCAAAGGTCTGCGTCTTCCACCACCCTCACCTTGTc                                                                                         <  6:321865/93‑1 (MQ=255)                                                                                                                      tGCTCTTTTTTTACCGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGGG‑‑CAATTATCAAAGGTCTGCGTCTTCCACCACCCTCACCTTGTc                                                                                         >  5:321865/1‑93 (MQ=255)                                                                                                                      tGCTCTTTTTTTACCGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGGG‑‑CAATTATCAAAGGTCTGCGTCTTCCACCACCCTCACCTTGTCTGCATCCAGCGCATAGGCCGCATCGGCCAGGTCATTGCTGACCTTCTCCACCTTCa                                 >  4:242782/1‑149 (MQ=255)                                                                                                                               tttACCGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGGG‑‑CAATTATCAAAGGTCTGCGTCTTCCACCACCCTCACCTTGTCTGCATCCAGCGCATAGGCCGCATCGGCCAGGTCATTGCTGACCTTCTCCACCTTCAGCTTGCCg                         <  5:16208/148‑1 (MQ=255)                                                                                                                                      gCCTCTTCGCGGGCTTGCCCGGGAAGAGGCCGGGG‑‑CAATTATCAAAGGTCTGCGTCTTCCCCCCCCCTCACCTGGTCTGCCTCCCGCGCATAGGCCGCTTCGGCCCGGTCATTGCTGCCGTTCTCCACCTTCAGCTTGCCGCTGACCca                 >  7:286483/1‑149 (MQ=255)                                                                                                                                        ctctTCGCGGGCAAGCCCGCGAAGAGGCCGGGG‑‑CAATTATCAAAGGTCTGCGTCTTCCACCACCCTCACCTTGTCTGCATCCAGCGCATAGGCCGCATCGGCCAGGTCATTGCTGACCTTCTCCACCTTCAg                                <  8:195272/132‑1 (MQ=255)                                                                                                                                        ctctTCGCGGGCAAGCCCGCGAAGAGGCCGGGG‑‑CAATTATCAAAGGTCTGCGTCTTCCACCACCCTCACCTTGTCTGCATCCAGCGCATAGGCCGCATCGGCCAGGTCATTGCTGACCTTCTCCACCTTCAg                                >  7:195272/1‑132 (MQ=255)                                                                                                                                        ctctTCGCGGGCAAGCCCGCGAAGAGGCCGGGG‑‑CAATTATCAAAGGTCTGCGTCTTCCACCACCCTCACCTTGTCTGCATCCAGCGCATAGGCCGCATCGGCCAGGTCATTGCTGACCTTCTCCACCTTCAGCTTGc                           >  7:159101/1‑137 (MQ=255)                                                                                                                                        ctctTCGCGGGCAAGCCCGCGAAGAGGCCGGGG‑‑CAATTATCAAAGGTCTGCGTCTTCCACCACCCTCACCTTGTCTGCATCCAGCGCATAGGCCGCATCGGCCAGGTCATTGCTGACCTTCTCCACCTTCAGCTTGc                           <  8:159101/137‑1 (MQ=255)                                                                                                                                         tctTCGCGGGCTTGCCCGCGAAGAGGCCGGGG‑‑CAATTATCAAAGGTCTGCGTCTTCCACCACCCTCACCTTGTCTGCATCCAGCGCATAGGCCGCATCGGCCAGGTCATTGCTGACCTTCTCCACCTTCAGCTTGCCGCTGACCCACAg              >  4:31769/1‑149 (MQ=255)                                                                                                                                         tctTCGCGGGCTTGCCCGCGAAGAGGCCGGGG‑‑CAATTATCAAAGGTCTGCGTCTTCCACCACCCTCACCTTGTCTGCATCCAGCGCATAGGCCGCATCGGCCAGGTCATTGCTGACCTTCTCCACCTTCAGCTTGCCGCTGACCCACAg              >  2:13766/1‑149 (MQ=255)                                                                                                                                         tctTCGCGGGCTTGCCCGCGAAGAGGCCGGGG‑‑CAATTATCAAAGGTCTGCGTCTTCCACCACCCTCACCTTGTCTGCATCCAGCGCATAGGCCGCATCGGCCAGGTCATTGCTGACCTTCTCCACCTTCAGCTTGCCGCTGACCCACAg              >  3:79736/1‑149 (MQ=255)                                                                                                                                           ttCGCGGGCAAGCCCGCGAAGAGGCCGGGG‑‑CAATTATCAAAGGTCTGCGTCTTCCACCACCCTCACCTTGTCTGCATCCAGCGCATAGGCCGCATCGGCCAGGTCATTGCTGACCTTCTCCACCTTCAGCTTGCCGCTGACCCACAGCg            >  4:370377/1‑149 (MQ=255)                                                                                                                                              gcgGGCTTGCCCGCGAAGAGGCCGGGG‑‑CAATTATCAAAGGTCTGCGTCTTCCACCACCCTCACCTTGTCTGCATCCAGCGCATAGGCCGCATCGGTCAGGTCATTGCTGACCTTCTCCACCTTCAGCTTGCCGCTGACCCACAGCggg          <  1:365767/148‑1 (MQ=255)                                                                                                                                              gcgGGCTTGCCCGCGAAGAGGCCGGGG‑‑CAATTATCAAAGGTCTGCGTCTTCCACCACCCTCACCTTGTCTGCATCCAGCGCATAGGCCGCATCGGCCAGGTCATTGCTGACCTTCTCCACCTTCAGCTTGCCGCTGACCCACAGCggg          <  3:344417/148‑1 (MQ=255)                                                                                                                                              gcgGGCTTGCCCGCGAAGAGGCCGGGG‑‑CAATTATCAAAGGTCTGCGTCTTCCACCACCCTCACCTTGTCTGCATCCAGCGCATAGGCCGCATCGGCCAGGTCATTGCTGACCTTCTCCACCTTCAGCTTGCCGCTGACCCACAGCggg          <  3:228771/148‑1 (MQ=255)                                                                                                                                                  gCTTGCCCGCGAAGAGGCCGGGG‑‑CAATTATCAAAGGTCTGCGTCTTCCACCACCCTCCCCTTGTCTGCATCCAGCGCATAGGCCGCATCGGCCAGGTCATTGCTGACCTTCTCCACCTTCAGCTTGCCGCTGACCCACAGCGGGgtg       >  4:331593/1‑147 (MQ=255)                                                                                                                                                    ttGCCCGCGAAGAGGCCGGGG‑‑CAATTATCAAAGGTCTGCGTCTTCCACCACCCTCACCTTGTCTGCATCCAGCGCATAGGCCGCATCGGCCAGGTCATTGCTGACCTTCTCCACCTTCAGCTTGCCGCTGACCCACAGCGGGgtg       >  5:229342/1‑145 (MQ=255)                                                                                                                                                    ttGCCCGCGAAGAGGCCGGGA‑‑CAATTATCAAAGGTCTGCGTCTTCCACCACCCTCACCTTGTCTGCATCCAGCGCATAGGCCGCATCGGCCAGGTCATTGCTGACCTTCTCCACCTTCAGCTTGCCGCTGACCCACAGCggg          <  4:326569/142‑1 (MQ=255)                                                                                                                                                    ttGCCCGCGAAGAGGCCGGGA‑‑CAATTATCAAAGGTCTGCGTCTTCCACCACCCTCACCTTGTCTGCATCCAGCGCATAGGCCGCATCGGCCAGGTCATTGCTGACCTTCTCCACCTTCAGCTTGCCGCTGACCCACAGCggg          >  3:326569/1‑142 (MQ=255)                                                                                                                                                     tGCCCGCGAAGAGGCCGGGG‑‑CAATTATCAAAGGTCTGCGTCTTCCACCACCCTCACCTTGTCTGCATCCAGCGCATAGGCCGCATCGGTCAGGTCATTGCGGACCTTCTCCACCTTCAGCTTGCCGCTGACCCACAGCGGGGTGTAGat  <  1:318992/149‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                       GATGGCAGCGAATGCTGTGCATCGAGACAGCGAATGTGTGGGATGACCTGGTGGAGTTGAAGCCTGGGGCGAGTCATTCGCTGGGCGTCCAGATCGGTTGTGAAAATATCTGAGCCCTGCTCTTTTTTTACCGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGGG‑‑CAATTATCAAAGGTCTGCGTCTTCCACCACCCTCACCTTGTCTGCATCCAGCGCATAGGCCGCATCGGCCAGGTCATTGCTGACCTTCTCCACCTTCAGCTTGCCGCTGACCCACAGCGGGGTGTAGAT  >  NC_002947/6080841‑6081137

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑